In Situ Gellable Oxidized Citrus Pectin for Localized Delivery of Anticancer Drugs and Prevention of Homotypic Cancer Cell Aggregation

In situ self-assembling of prodrug molecules into nanomedicine can elevate the therapeutic efficacy of anticancer medications by enhancing the targeting and enrichment of anticancer drugs at tumor sites. However, the...

Download Full-text

Saudi anti-human cancer plants database (SACPD): A collection of plants with anti-human cancer activities

Oncology Reviews ◽

10.4081/oncol.2018.349 ◽

2018 ◽

Cited By ~ 1

Author(s):

Ateeq Ahmed Al-Zahrani

Keyword(s):

Saudi Arabia ◽

Cell Lines ◽

Anticancer Drugs ◽

Plant Species ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Lines ◽

Anticancer Activities ◽

Excellent Starting Point ◽

Starting Point

Several anticancer drugs have been developed from natural products such as plants. Successful experiments in inhibiting the growth of human cancer cell lines using Saudi plants were published over the last three decades. Up to date, there is no Saudi anticancer plants database as a comprehensive source for the interesting data generated from these experiments. Therefore, there was a need for creating a database to collect, organize, search and retrieve such data. As a result, the current paper describes the generation of the Saudi anti-human cancer plants database (SACPD). The database contains most of the reported information about the naturally growing Saudi anticancer plants. SACPD comprises the scientific and local names of 91 plant species that grow naturally in Saudi Arabia. These species belong to 38 different taxonomic families. In Addition, 18 species that represent16 family of medicinal plants and are intensively sold in the local markets in Saudi Arabia were added to the database. The website provides interesting details, including plant part containing the anticancer bioactive compounds, plants locations and cancer/cell type against which they exhibit their anticancer activity. Our survey revealed that breast, liver and leukemia were the most studied cancer cell lines in Saudi Arabia with percentages of 27%, 19% and 15%, respectively. The current SACPD represents a nucleus around which more development efforts can expand to accommodate all future submissions about new Saudi plant species with anticancer activities. SACPD will provide an excellent starting point for researchers and pharmaceutical companies who are interested in developing new anticancer drugs. SACPD is available online at https://teeqrani1.wixsite.com/sapd

Download Full-text

In Situ Cross-Linkable High Molecular Weight Hyaluronan−Bisphosphonate Conjugate for Localized Delivery and Cell-Specific Targeting: A Hydrogel Linked Prodrug Approach

Journal of the American Chemical Society ◽

10.1021/ja902857b ◽

2009 ◽

Vol 131 (25) ◽

pp. 8781-8783 ◽

Cited By ~ 91

Author(s):

Oommen P. Varghese ◽

Weilun Sun ◽

Jöns Hilborn ◽

Dmitri A. Ossipov

Keyword(s):

Molecular Weight ◽

High Molecular Weight ◽

Specific Targeting ◽

Localized Delivery

Download Full-text

Capturing quantitative features of protein expression from in situ fluorescence microscopic images of cancer cell populations

Fluorescence Imaging and Biological Quantification ◽

10.1201/9781315121017-15 ◽

2017 ◽

pp. 279-297

Author(s):

Joana Figueiredo ◽

Ana Sofia Ribeiro ◽

Tânia Mestre ◽

Sofia Esménio ◽

Martina Fonseca ◽

...

Keyword(s):

Protein Expression ◽

Cancer Cell ◽

Cell Populations ◽

Microscopic Images

Download Full-text

Establishment and Characterization of a Novel Multidrug Resistant Human Ovarian Cancer Cell Line With Heterogenous MRP7 Overexpression

Frontiers in Oncology ◽

10.3389/fonc.2021.731260 ◽

2021 ◽

Vol 11 ◽

Author(s):

Jing-Quan Wang ◽

Zhuo-Xun Wu ◽

Yuqi Yang ◽

Jin-Sui Li ◽

Dong-Hua Yang ◽

...

Keyword(s):

Ovarian Cancer ◽

Multidrug Resistance ◽

Cell Line ◽

Anticancer Drugs ◽

Cancer Cell ◽

Ovarian Cancer Cell ◽

Ovarian Cancer Cell Line ◽

Cancer Cell Line ◽

Chemotherapeutic Drugs ◽

Mesenchymal Transition

Ovarian cancer is one of the leading female malignancies which accounts for the highest mortality rate among gynecologic cancers. Surgical cytoreduction followed by chemotherapy is the mainstay of treatment. However, patients with recurrent ovarian cancer are likely to exhibit resistance to chemotherapy due to reduced sensitivity to chemotherapeutic drugs. Adenosine triphosphate (ATP)-binding cassette (ABC) transporters have been extensively studied as multidrug resistance (MDR) mediators since they are responsible for the efflux of various anticancer drugs. Multidrug resistance protein 7 (MRP7, or ABCC10) was discovered in 2001 and revealed to transport chemotherapeutic drugs. Till now, only limited knowledge was obtained regarding its roles in ovarian cancer. In this study, we established an MRP7-overexpressing ovarian cancer cell line SKOV3/MRP7 via transfecting recombinant MRP7 plasmids. The SKOV3/MRP7 cell line was resistant to multiple anticancer drugs including paclitaxel, docetaxel, vincristine and vinorelbine with a maximum of 8-fold resistance. Biological function of MRP7 protein was further determined by efflux-accumulation assays. Additionally, MTT results showed that the drug resistance of the SKOV3/MRP7 cells was reversed by cepharanthine, a known inhibitor of MRP7. Moreover, we also found that the overexpression of MRP7 enhanced the migration and epithelial-mesenchymal transition (EMT) induction. In conclusion, we established an in vitro model of MDR in ovarian cancer and suggested MRP7 overexpression as the leading mechanism of chemoresistance in this cell line. Our results demonstrated the potential relationship between MRP7 and ovarian cancer MDR.

Download Full-text

Mitotic arrest affects clustering of tumor cells

10.21203/rs.3.rs-47408/v3 ◽

2021 ◽

Author(s):

Julia Bonnet ◽

Lise Rigal ◽

Odile Mondesert ◽

Renaud Morin ◽

Gaelle Corsaut ◽

...

Keyword(s):

Tumor Cells ◽

Circulating Tumor Cells ◽

Cancer Cell ◽

Cell Aggregation ◽

Metastatic Potential ◽

Mitotic Arrest ◽

Chemotherapeutic Agents ◽

The Impact ◽

Mcf 7

Abstract Background Cancer cell aggregation is a key process involved in the formation of tumor cell clusters. It has recently been shown that clusters of circulating tumor cells (CTCs) have an increased metastatic potential compared to isolated circulating tumor cells. Several widely used chemotherapeutic agents that target the cytoskeleton microtubules and cause cell cycle arrest at mitosis have been reported to modulate CTC number or the size of CTC clusters. Results In this study, we investigated in vitro the impact of mitotic arrest on the ability of breast tumor cells to form clusters. By using live imaging and quantitative image analysis, we found that MCF-7 cancer cell aggregation is compromised upon incubation with paclitaxel or vinorelbine, two chemotherapeutic drugs that target microtubules. In line with these results, we observed that MCF-7 breast cancer cells experimentally synchronized and blocked in metaphase aggregated poorly and formed loose clusters. To monitor clustering at the single-cell scale, we next developed and validated an in vitro assay based on live video-microscopy and custom-designed micro-devices. The study of cluster formation from MCF-7 cells that express the fluorescent marker LifeAct-mCherry using this new assay allowed showing that substrate anchorage-independent clustering of MCF-7 cells was associated with the formation of actin-dependent highly dynamic cell protrusions. Metaphase-synchronized and blocked cells did not display such protrusions, and formed very loose clusters that failed to compact. Conclusions Altogether, our results suggest that mitotic arrest induced by microtubule-targeting anticancer drugs prevents cancer cell clustering and therefore, could reduce the metastatic potential of circulating tumor cells.

Download Full-text

Intrinsic growth heterogeneity of mouse leukemia cells underlies differential susceptibility to a growth-inhibiting anticancer drug

10.1101/2020.07.10.196881 ◽

2020 ◽

Author(s):

Akihisa Seita ◽

Hidenori Nakaoka ◽

Reiko Okura ◽

Yuichi Wakamoto

Keyword(s):

Cancer Cells ◽

Anticancer Drugs ◽

Cancer Cell ◽

Anticancer Drug ◽

Differential Susceptibility ◽

Leukemia Cells ◽

Continuous Exposure ◽

Phenotypic Differences ◽

Cell Lineages ◽

Slow Cycling

AbstractCancer cell populations consist of phenotypically heterogeneous cells. Growing evidence suggests that pre-existing phenotypic differences among cancer cells correlate with differential susceptibility to anticancer drugs and eventually lead to a relapse. Such phenotypic differences can arise not only externally driven by the environmental heterogeneity around individual cells but also internally by the intrinsic fluctuation of cells. However, the quantitative characteristics of intrinsic phenotypic heterogeneity emerging even under constant environments and their relevance to drug susceptibility remain elusive. Here we employed a microfluidic device, mammalian mother machine, for studying the intrinsic heterogeneity of growth dynamics of mouse lymphocytic leukemia cells (L1210) across tens of generations. The generation time of this cancer cell line had a distribution with a long tail and a heritability across generations. We determined that a minority of cell lineages exist in a slow-cycling state for multiple generations. These slow-cycling cell lineages had a higher chance of survival than the fast-cycling lineages under continuous exposure to the anticancer drug Mitomycin C. This result suggests that heritable heterogeneity in cancer cells’ growth in a population influences their susceptibility to anticancer drugs.

Download Full-text