Combination of Vitamin E and Folic Acid Ameliorate Oxidative Stress and Apoptosis in Diabetic Rat Uterus

2014 ◽  
Vol 84 (1-2) ◽  
pp. 55-64 ◽  
Author(s):  
Fatemeh Zal ◽  
Zeinab Mahdian ◽  
Razieh Zare ◽  
Soghra Bahmanpour ◽  
Zohreh Mostafavi-Pour
Keyword(s):  
Folic Acid ◽  
Vitamin E ◽  
Oxidative Damage ◽  
Olive Oil ◽  
Caspase 3 ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Uterine Tissue ◽  
Mda Content

This study was designed to assess oxidative damage and cell apoptosis in the uterus of rats with streptozotocin (STZ)-induced diabetes. The role of vitamin E (VE) and/or folic acid (FA) in the protection from such damage was also evaluated. The treatments were performed for 4 weeks on six groups of rats: 1) normal control 2) diabetic control 3) diabetic rats receiving olive oil as a vehicle (without VE) 4) diabetic rats treated with VE (200 mg/kg) in olive oil 5) diabetic rats treated with FA (25 mg/kg) and 6) diabetic rats treated with VE + FA (200 and 25 mg/kg, respectively). We measured the malondialdehyde level (MDA), glutathione content (GSH) and the activity of GSH peroxidase (GPx), GSH reductase (GR) and catalase. Changes in caspase-3 activity were quantified in uterine tissue to assess the rate of apoptosis. In the rat uterine tissues, MDA content and caspase-3 activity were significantly elevated, while GPx, GR and CAT activities and the GSH level were significantly decreased in the diabetic control compared with those in normal rats (p < 0.05). The combination of the vitamins (VE + FA) restored uterine GSH content and enzymatic activities of GPx, GR and CAT and reduced the MDA level (p < 0.05). A prominent reduction in apoptosis of uterine cells was detected in diabetic rats treated with two vitamins (p < 0.05). Overall, VE alone, not FA, produced results similar to those of the VE + FA combination. Thus, in the uterine tissue of diabetic rats, diabetes complications (that are caused by oxidative damage and apoptosis induction) can be prevented by the systemic administration of VE and FA.

scholarly journals Quercetin and Vitamin E ameliorate cardio-apoptotic risks in diabetic rats

2021 ◽  
Author(s):  
Oluwatoyin Ojo ◽  
Ifedayo M. Obaidu ◽  
Oluwatosin C. Obigade ◽  
Olufunso O. Olorunsogo
Keyword(s):  
Vitamin E ◽  
Cytochrome C ◽  
Caspase 3 ◽  
Permeability Transition ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Cytochrome C Release ◽  
Rat Hearts ◽  
Apoptotic Effect ◽  
Low Ionic Strength

Abstract Background: Apoptosis is upregulated in all forms of diabetes, with the mitochondria acting as a target in diabetes pathophysiology. Quercetin and vitamin E have shown usefulness in the delay of progression of diabetes-induced complications. However, their effect on the apoptotic process in diabetes mellitus is unknown. We hypothesize that quercetin treatment in diabetes may decrease the propensity for cardio-myocytic death via regulation of the mitochondria permeability transition (mPT) pore opening. Methods: Hearts from normal and streptozotocin-induced diabetic rats were used for the study. Low-ionic strength heart mitochondria were used for swelling assay, and mitochondrial lipid peroxidation (mLPO) activity was spectrophotometrically assessed. Levels of cytochrome c, caspase 3 and 9 were determined by immunohistochemistry, while lesions assessed by histology.Discussion: Diabetic heart mPT pore showed larger amplitude swelling than control, while mLPO levels was increased in diabetic rats relative to control, this resulted in cytochrome c release. This initiated increased caspase 3 and 9 activity in diabetic rats (p<0.05). Histology showed haemorrphagic lesions in diabetic rat hearts. Quercetin and vitamin E treatment reversed these effects, suggestive of their anti-apoptotic effect. Conclusions: Quercetin and vitamin E protection in diabetes is mediated by mPT pore inhibition and modulation of mitochondrial-mediated apoptosis.

KELULUT HONEY SUPPLEMENTATION PREVENTS SPERM AND TESTICULAR OXIDATIVE DAMAGE IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

2017 ◽  
Vol 79 (3) ◽  
Author(s):  
Siti Balkis Budin ◽  
Fatin Farhana Jubaidi ◽  
Siti Nur Farahana Mohd Noor Azam ◽  
Nur Liyana Mohamed Yusof ◽  
Izatus Shima Taib ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Sperm Quality ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Control Groups ◽  
Sod Activity ◽  
Male Adult ◽  
Pathological Conditions ◽  
Sprague Dawley Rats

Previous studies found that Kelulut Honey produced by Trigona spp. bees is able to prevent oxidative damage in various pathological conditions.  Thus, the present study aimed to determine whether Kelulut Honey could prevent the sperm and testicular damage in streptozotocin-induced diabetic rats. Male Adult male Sprague-Dawley rats were divided into four groups: Non-Diabetic (NDM), Non-Diabetic with Kelulut Honey supplementation (NDMKH), Diabetic without supplementation (DM) and Diabetic with Kelulut Honey supplementation (DMKH).  Kelulut honey was given at the dose of 2.0 g/kg weight daily via gavage for 28 consecutive days. Results showed that sperm quality produced by diabetic rats supplemented with Kelulut honey significantly improved compared to the diabetic control groups (p<0.05). SOD activity and GSH level increased significantly (p<0.05) whereas PC and MDA levels significantly decreased in sperm and testis of DMKH rats when compared to DM rats (p<0.05). Histological observation showed obvious increase in spermatozoa in the lumen of epididymis and increased spermatogenic cells density in the testis of DMKH group.  In conclusion, Kelulut Honey has a potential in preventing the damage of sperm and testis in diabetic rats.

scholarly journals Antidiabetic potential of the leaf extract of Centella asiatica in alloxaninduced diabetic rats

2016 ◽  
Vol 4 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Talha Bin Emran ◽  
Mycal Dutta ◽  
Mir Muhammad Nasir Uddin ◽  
Aninda Kumar Nath ◽  
Md Zia Uddin
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Total Cholesterol Level ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Liver Glycogen ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat

The study was designed to evaluate the glucose and cholesterol lowering effect of the aqueous extract of Centella asiatica leaf using the alloxan-induced diabetic rats and compared the activity with diabetic control and antidiabetic drug (Glibenclamide). Leaf extract (50 mg/kg) of C. asiatica and Glibenclamide were administered to normal and experimental diabetic rats for the duration of 10 days. In the alloxan-induced diabetic rat model, C. asiatica extract (50 mg/kg) significantly (p < 0.05) lowered the fasting blood glucose level as well as the total cholesterol level. Serum insulin levels were not stimulated in the animals treated with the extract. In addition, changes in body weight, serum lipid profiles and liver glycogen levels assessed in the extract treated diabetic rats were compared with diabetic control and normal animals. Significant results (p < 0.05) were observed in the estimated parameters. Surprisingly, body weight was increased significantly (p < 0.05) in the C. asiatica treated diabetic group. Phytochemical screening showed the presence of alkaloids, flavonoids, glycosides, steroids and tannins in significant amountsJahangirnagar University J. Biol. Sci. 4(1): 51-59, 2015 (June)

scholarly journals PO-298 Effect of exercise and Siyeshen upon hippocampal cytochrome c and caspase-3 of diabetic rats

2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Dongmei Wang ◽  
Jinming Zhang ◽  
Haibin Liu ◽  
Rongmei Wang
Keyword(s):  
Cytochrome C ◽  
Protein Expression ◽  
Caspase 3 ◽  
Diabetic Control ◽  
Exercise Group ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Mrna And Protein Expression ◽  
Cyt C

Objective To observe the effects of aerobic exercise and Siyeshen water extract on cytochrome c (Cyt c) and caspase-3 in hippocampus of diabetic rats and to explore the possible mechanism of improving diabetes. Methods Healthy male Wister rats fed with high fat and high sugar and combined with streptozotocin to establish type II diabetes model. They were randomly divided into 4 groups: diabetic control group, exercise group, Siyeshen group and exercise+Siyeshen group, and another normal control group, with 6 rats in each group. After aerobic exercise (15m/min, 5°slope, 60min, every other day) or/and Siyeshen (200mg/kg) gastrointestinal administration for 8w, the expression of Cyt c and caspase-3 in hippocampus of each group were detected by immunoblotting, and mRNA expressions were detected by RT-PCR. Results Compared with the normal control group, the mRNA and protein expressions of Cyt c and caspase-3 in the hippocampus of the diabetic control group were significantly increased (P<0.05). Compared with the diabetic control group, the blood glucose level of exercise group and exercise+ Siyeshen group decreased (P<0.05), the mRNA and protein expression of Cyt c and caspase-3 decreased significantly (P<0.05), but there were no significant changes in the mRNA and protein expression of Cyt c and caspase-3 between Siyeshen group and diabetic control group (P﹥0.05). Conclusions Exercise and exercise combined with Siyeshen can inhibit cytochrome c release and reduce caspase-3 protein expression, which may be related to the improvement of blood glucose levels in diabetic rats.

scholarly journals Effects of Brown Seaweed (Sargassum polycystum) Extracts on Kidney, Liver, and Pancreas of Type 2 Diabetic Rat Model

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Mahsa Motshakeri ◽  
Mahdi Ebrahimi ◽  
Yong Meng Goh ◽  
Hemn Hassan Othman ◽  
Mohd Hair-Bejo ◽  
...  
Keyword(s):  
Body Weight ◽  
Brown Seaweed ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Edible Seaweed ◽  
Sargassum Polycystum ◽  
Liver And Kidney ◽  
Type 2 Diabetic

The edible seaweedSargassum polycystum(SP) is traditionally used against several human diseases. This investigation evaluated the effects of two dietary doses of SP ethanolic and aqueous extracts on the pancreatic, hepatic, and renal morphology of type 2 diabetic rats (T2DM). T2DM was induced by feeding rats on high calorie diet followed by a low dose streptozotocin. Changes in the diabetic rat organs in SP treated groups with different doses of extracts were compared with normal rats, diabetic control rats, and metformin treated rats. After 22 days of treatment, the pathological lesions of the livers and kidneys in the diabetic rats were quantitatively and qualitatively alleviated (P<0.05) by both the SP extracts at 150 mg/kg body weight and by metformin. All the treated diabetic groups revealed marked improvement in the histopathology of the pancreas compared with the control diabetic group. Oral administration of 300 mg/kg body weight of aqueous and ethanolic extracts of SP and metformin revealed pancreas protective or restorative effects. The seaweed extracts at 150 mg/kg body weight reduced the liver and kidney damages in the diabetic rats and may exert tissue repair or restoration of the pancreatic islets in experimentally induced diabetes to produce the beneficial homeostatic effects.

scholarly journals ANTI-HYPERGLYCEMIC EFFECT OF TERMINALIA CATAPPA FRUIT EXTRACT IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

2017 ◽  
Vol 9 (4) ◽  
pp. 212 ◽  
Author(s):  
Tapan Behl ◽  
Anita Kotwani
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Urine Volume ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Dependent Manner ◽  
Fruit Extract ◽  
Terminalia Catappa ◽  
Hyperglycemic Effect

Objective: To explore the anti-hyperglycemic effect of fruit extract of Terminalia catappa (Indian almond), a potential medicine from plant origin in a diabetic rat model.Methods: Streptozotocin-induced chronic diabetic rat model was utilized in the study. Three doses of test drug, hydro-alcoholic fruit extract of Terminalia catappa in 20 mg/kg, 30 mg/kg and 40 mg/kg and a standard anti-diabetic drug, glibenclamide (10 mg/kg) was used. The study had a total of nine groups with eight animals in each group. Drugs were given orally every day for 12 w. Blood glucose, body weight and urine volume were measured weekly, glycosylated hemoglobin (HbA1c) was estimated at 12th week in all groups. Data for all parameters were analyzed using one-way ANOVA repeated measures followed by Mann-Whitney test.Results: Hydro-alcoholic fruit extract of T. catappa significantly decreased blood glucose, urine volume and increased body weight in a dose-dependent manner in diabetic rats. At 12th week, blood glucose level in control, diabetic control, glibenclamide, T. catappa (40 mg/kg) group was 96.25±2.05 mg/dl, 599.75±0.25 mg/dl, 248.25±11.45 mg/dl, 115.00±3.78 mg/dl respectively. Effect of T. catappa in 30 mg/kg and 40 mg/kg dose was significantly more than glibenclamide. At 12th week, HbA1c level in control, diabetic control, glibenclamide, T. catappa (40 mg/kg) was 2.94±0.33 mmol/l, 4.94±0.49 mmol/l, 3.61±0.28 mmol/l, 3.21±0.27 mmol/l. Treatment with T. catappa 30 mg/kg, 40 mg/kg and glibenclamide brought back the level of HbA1c to normal levels. The addition of glibenclamide to T. catappa (40 mg/kg) did not produce any additional effect on blood glucose and HbA1c levels compared to the effect of T. catappa (40 mg/kg) in diabetic rats.Conclusion: Terminalia catappa fruit extract exhibited a significant anti-hyperglycemic effect in diabetic rats and has a great potential to be used in diabetes.

scholarly journals Stellaria media attenuates the hyperglycemia and hyperlipidemia in alloxan-induced diabetic rat

2019 ◽  
Vol 14 (2) ◽  
pp. 80-86 ◽  
Author(s):  
Rahmat Khan ◽  
Wasim Ahmad ◽  
Mushtaq Ahmed
Keyword(s):  
Research Work ◽  
Diabetic Control ◽  
Experimental Models ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Serum Enzyme ◽  
Stellaria Media ◽  
Diabetic Treatment

Abstract The objective of this research work was to assess the hyperglycemic and hyperlipidemiceffects of Stellaria media in alloxan induced diabetic rats using different experimental models. Standard documented protocols were used to concede the in vitro and in vivo activities. Biochemical markers studies were also done. The results of the study showed strong pancreatic α-amylase and β-glucosidase inhibition in-vitroat varying concentrations of the extract which further validated the in-vivo anti-diabetic action of the plant because of the inhibition of the above enzymes.The administration of various concentrations of the extract showedmomentous decrease in fasting blood level when compared to diabetic control. Similarly, remarkably improved hemoglobin (+20.10%), and decreased HbA1c (−48.44%) was observed when compared to diabetic control rats. The extract also caused reduced serum enzyme (ALT, ALP, bilirubin) levels and produced a succeeding recovery toward their normal values.It can be concluded from these investigations that the in-vitro and in-vivo hypoglycemic and hypolipidemic activity offers the methodicaljustification for the use of S. media in herb based anti-diabetic treatment.

scholarly journals Role of Hyperketonemia in Inducing Oxidative Stress and Cellular Damage in Cultured Hepatocytes and Type 1 Diabetic Rat Liver

2015 ◽  
Vol 37 (6) ◽  
pp. 2160-2170 ◽  
Author(s):  
Preeti Kanikarla-Marie ◽  
Sushil K. Jain
Keyword(s):  
Oxidative Stress ◽  
Liver Damage ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Cellular Damage ◽  
Diabetic Rat ◽  
Blood Levels ◽  
Per Se ◽  
Rat Livers

Background/Aims: Type 1 diabetic (T1D) patients have a higher incidence of liver disease. T1D patients frequently experience elevated plasma ketone levels along with hyperglycemia. However, no study has examined whether hyperketonemia per se has any role in excess liver damage in T1D. This study investigates the hypothesis that hyperketonemia can induce oxidative stress and cellular dysfunction. Methods: STZ treated diabetic rats, FL83B hepatocytes, and GCLC knocked down (GSH deficient) hepatocytes were used. Results: The blood levels of ALT and AST, biomarkers of liver damage, and ketones were elevated in T1D rats. An increase in NOX4 and ROS along with a reduction in GSH and GCLC levels was observed in T1D rat livers in comparison to those seen in non-diabetic control or type 2 diabetic rats. MCP-1 and ICAM-1 were also elevated in T1D rat livers and ketone treated hepatocytes. Macrophage markers CCR2 and CD11A that interact with MCP-1, and ICAM-1 respectively, were also elevated in the T1D liver, indicating macrophage infiltration. Additionally, activated macrophages increased hepatocyte damage with ketone treatment, which was similar to that seen in GCLC knockdown hepatocytes without ketones. Conclusion: Hyperketonemia per se can induce macrophage mediated damage to hepatocytes and the liver, caused by GSH depletion and oxidative stress up regulation in T1D.

scholarly journals The effect of kefir-spirulina on glycemic status and antioxidant activity in hyperglycemia rats

10.5219/1445 ◽  
2021 ◽  
Vol 15 ◽  
pp. 101-110
Author(s):  
Nur Laela ◽  
Anang Mohamad Legowo ◽  
Faizah Fulyani
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Normal Control ◽  
Plasma Glucose ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Nutritional Content ◽  
Glycemic Status ◽  
Chronic Hyperglycemia

Diabetes mellitus (DM) is a metabolic disorder characterized by chronic hyperglycemia. It is caused by impaired insulin secretion or by insulin receptor insensitivity. DM and its complications are often related to increases in the level of oxidative stress. Spirulina is a nutrient-dense food that contains an abundance of antioxidant compounds. In combination with kefir, it may serve as both a nutrient-rich diet and an antioxidant agent that can prevent complications of diabetes. This study aims to investigate the nutritional content of kefir-spirulina and its effect on glycemic status and antioxidant activity in streptozotocin-nicotinamide (STZ-NA) induced diabetic rats. A total of 30 male Sprague Dawley rats were divided into five groups: normal control (K1), diabetic control (K2), pioglitazone treatment (K3), kefir combined with 1% spirulina treatment (P1), and kefir combined with 2% spirulina treatment (P2). All rats were induced by STZ-NA, except for the normal control. Before and after the 28 days of intervention, blood samples were taken and analyzed for fasting plasma glucose, postprandial glucose, and SOD activity. The nutritional content, ethanol content, and total antioxidant capacity of kefir-spirulina were also analyzed. The diabetic rats that were fed with kefir-spirulina (P1 and P2) had a significant decrease in both fasting and postprandial plasma glucose (p <0.001) compared to the diabetic control rats. The decrease of plasma glucose in K2 is comparable to the control rats treated with the diabetic drug pioglitazone (K3). The activity of SOD in diabetic rats fed in P1 and P2 were higher (p <0.001) than in untreated diabetic rats (K2). The IC50 of kefir-spirulina was 42 – 43 ppm. It was concluded that kefir combined with spirulina has high nutrition and antioxidant capacity, which is proven to be capable of controlling glycemic status and enhancing antioxidant status in a diabetic rat model.

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