Differential protein profiling of soil diazotroph Rhodococcus qingshengii S10107 towards low-temperature and nitrogen deficiency

AbstractProtein-based biomarkers can be a promising approach for identification and real-time monitoring of the bio-inoculants employed under sustainable agricultural plans. In this perspective, differential proteomics of psychrophilic diazotroph Rhodococcus qingshengii S10107 (JX173283) was performed to unravel its adaptive responses towards low-temperature nitrogen deficiency and identification of a biomarker for respective physiological conditions. LC-MS/MS-based proteome analysis mapped more than 4830 proteins including 77 up-regulated and 47 down-regulated proteins (p ≤ 0.05). Differential expression of the structural genes of nif regulon viz. nifH, nifD, and nifK along with their response regulators i.e. nifA, nifL, and nifB indicated that the nitrogenase complex was activated successfully. Besides up-regulating the biosynthesis of certain amino acids viz. Leucine, Lysine, and Alanine; the expression of the peptidoglycan synthesis proteins were also increased; while, the enzymes involved in Lipid biosynthesis were found to decrease. Furthermore, two important enzymes of the pentose phosphate pathway viz. Transketolase and Transaldolase along with Ribose import ATP-binding protein RbsA were also found to induce significantly under low temperature a nitrogen deficient condition, which suggests the cellular need for ample ribose sugar instantly. Additionally, comparative protein profiling of S10107 strain with our previous studies revealed that CowN protein was significantly up-regulated in all the cases under low-temperature nitrogen deficient conditions and therefore, can be developed as a biomarker. Conclusively, present study for the first time provides an in-depth proteome profiling of R. qingshengii S10107 and proclaims CowN as a potential protein biomarker for monitoring BNF under cold niches.

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Transformation behavior of yttria stabilized tetragonal zirconia polycrystal–TiB2 composites

Journal of Materials Research ◽

10.1557/jmr.2001.0294 ◽

2001 ◽

Vol 16 (7) ◽

pp. 2158-2169 ◽

Cited By ~ 21

Author(s):

B. Basu ◽

J. Vleugels ◽

O. Van Der Biest

Keyword(s):

Thermal Expansion ◽

Phase Transformation ◽

Low Temperature ◽

Tetragonal Zirconia ◽

Mechanical Analysis ◽

Transformation Behavior ◽

Thermal Expansion Data ◽

Thermally Induced ◽

First Time ◽

Tetragonal Zirconia Polycrystal

The objective of the present article is to study the influence of TiB2 addition on the transformation behavior of yttria stabilized tetragonal zirconia polycrystals (Y-TZP). A range of TZP(Y)–TiB2 composites with different zirconia starting powder grades and TiB2 phase contents (up to 50 vol%) were processed by the hot-pressing route. Thermal expansion data, as obtained by thermo-mechanical analysis were used to assess the ZrO2 phase transformation in the composites. The thermal expansion hysteresis of the transformable ceramics provides information concerning the transformation behavior in the temperature range of the martensitic transformation and the low-temperature degradation. Furthermore, the transformation behavior and susceptibility to low-temperature degradation during thermal cycling were characterized in terms of the overall amount and distribution of the yttria stabilizer, zirconia grain size, possible dissolution of TiB2 phase, and the amount of residual stress generated in the Y-TZP matrix due to the addition of titanium diboride particles. For the first time, it is demonstrated in the present work that the thermally induced phase transformation of tetragonal zirconia in the Y-TZP composites can be controlled by the intentional addition of the monoclinic zirconia particles into the 3Y-TZP matrix.

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CO2 hydrogenation into CH4 over Ni–Fe catalysts

Functional Materials Letters ◽

10.1142/s1793604718500571 ◽

2018 ◽

Vol 11 (03) ◽

pp. 1850057 ◽

Cited By ~ 8

Author(s):

Reza Meshkini Far ◽

Olena V. Ischenko ◽

Alla G. Dyachenko ◽

Oleksandr Bieda ◽

Snezhana V. Gaidai ◽

...

Keyword(s):

Low Temperature ◽

Synergistic Effect ◽

Atmospheric Pressure ◽

High Efficiency ◽

Co2 Hydrogenation ◽

Hydrogenation Catalysts ◽

Fe Catalysts ◽

First Time ◽

Supported Ni ◽

Hydrogenation Of Co

Here, we report, for the first time, on the catalytic hydrogenation of CO2 to methane at atmospheric pressure. For the preparation of hydrogenation catalysts based on Ni and Fe metals, a convenient method is developed. According to this method, low-temperature reduction of the co-precipitated Ni and Fe oxides with hydrogen gives the effective and selective bimetallic Ni[Formula: see text]Fe[Formula: see text], Ni[Formula: see text]Fe[Formula: see text] and Ni[Formula: see text]Fe[Formula: see text] catalysts. At the temperature range of 300–400[Formula: see text]C, they exhibit a high efficiency of CH4 production with respect to monometallic Ni and Fe catalysts. The results imply a synergistic effect between Ni and Fe which caused the superior activity of the Ni[Formula: see text]Fe[Formula: see text] catalyst conversing [Formula: see text]% of CO2 into CH4 at 350[Formula: see text]C. To adapt the Ni–Fe catalysts in the industry, the effect of two different carriers on the efficiency of the alumina-supported Ni[Formula: see text]Fe[Formula: see text] catalyst was investigated. It is found that the Ni[Formula: see text]Fe[Formula: see text]/[Formula: see text]-Al2O3 catalyst effectively conversed CO2 giving 100% methane yield already at 275[Formula: see text]C.

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Gold in Irish Coal: Palaeo-Concentration from Metalliferous Groundwaters

Minerals ◽

10.3390/min10070635 ◽

2020 ◽

Vol 10 (7) ◽

pp. 635

Author(s):

Liam A. Bullock ◽

John Parnell ◽

Joseph G.T. Armstrong ◽

Magali Perez ◽

Sam Spinks

Keyword(s):

Heat Flow ◽

Low Temperature ◽

Precious Metals ◽

High Heat ◽

Selenium Content ◽

Foreland Basins ◽

High Heat Flow ◽

South Wales ◽

First Time ◽

Very High

Gold grains, up to 40 μm in size and containing variable percentages of admixed platinum, have been identified in coals from the Leinster Coalfield, Castlecomer, SE Ireland, for the first time. Gold mineralisation occurs in sideritic nodules in coals and in association with pyrite and anomalous selenium content. Mineralisation here may have reflected very high heat flow in foreland basins north of the emerging Variscan orogenic front, responsible for gold occurrence in the South Wales Coalfield. At Castlecomer, gold (–platinum) is attributed to precipitation with replacive pyrite and selenium from groundwaters at redox interfaces, such as siderite nodules. Pyrite in the cores of the nodules indicates fluid ingress. The underlying Caledonian basement bedrock is mineralised by gold, and thus likely provided a source for gold. The combination of the gold occurrences in coal in Castlecomer and in South Wales, proximal to the Variscan orogenic front, suggests that these coals along the front could comprise an exploration target for low-temperature concentrations of precious metals.

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First comprehensive proteome analysis of lysine crotonylation in Streptococcus agalactiae, a pathogen causing meningoencephalitis in teleosts

Proteome Science ◽

10.1186/s12953-021-00182-y ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Xinjin Chen ◽

Bolin Fan ◽

Chenlong Fan ◽

Zhongliang Wang ◽

Eakapol Wangkahart ◽

...

Keyword(s):

Streptococcus Agalactiae ◽

Proteome Analysis ◽

Post Translational Modifications ◽

Sensing System ◽

Affinity Enrichment ◽

Single Organism ◽

Starting Point ◽

Zoonotic Risk ◽

Quorum Sensing System ◽

First Time

Abstract Backgroud Streptococcus agalactiae is a common colonizer of the rectovaginal tract and lead to infectious diseases of neonatal and non-pregnant adults, which also causes infectious disease in fish and a zoonotic risk as well. Lysine crotonylation (Kcr) is a kind of histone post-translational modifications discovered in 2011. In yeast and mammals, Kcr function as potential enhancers and promote gene expression. However, lysine crotonylation in S. agalactiae has not been studied yet. Methods In this study, the crotonylation profiling of fish pathogen, S. agalactiae was investigated by combining affinity enrichment with LC MS/MS. The Kcr modification of several selected proteins were further validated by Western blotting. Results In the present study, we conducted the proteome-wide profiling of Kcr in S. agalactiae and identified 241 Kcr sites from 675 screened proteins for the first time. Bioinformatics analysis showed that 164 sequences were matched to a total of six definitively conserved motifs, and many of them were significantly enriched in metabolic processes, cellular process, and single-organism processes. Moreover, four crotonylation modified proteins were predicted as virulence factors or to being part of the quorum sensing system PTMs on bacteria. The data are available via ProteomeXchange with identifier PXD026445. Conclusions These data provide a promising starting point for further functional research of crotonylation in bacterial virulence in S. agalactiae.

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Low-temperature and purification-free stereocontrolled ring-opening polymerisation of lactide in supercritical carbon dioxide

Green Chemistry ◽

10.1039/d0gc00352b ◽

2020 ◽

Vol 22 (7) ◽

pp. 2197-2202 ◽

Cited By ~ 3

Author(s):

Simon P. Bassett ◽

Andrew D. Russell ◽

Paul McKeown ◽

Isabel Robinson ◽

Thomas R. Forder ◽

...

Keyword(s):

Carbon Dioxide ◽

Low Temperature ◽

Supercritical Carbon Dioxide ◽

Ring Opening ◽

Solvent Free ◽

Free Ring ◽

First Time ◽

Supercritical Carbon

A stereoselective, solvent-free ring-opening polymerisation (ROP) of lactide (LA) in supercritical carbon dioxide (scCO2) is reported for the first time.

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Isolation and Characterization of a Biosurfactant Producing Strain Planococcus sp. XW-1 from the Cold Marine Environment

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph19020782 ◽

2022 ◽

Vol 19 (2) ◽

pp. 782

Author(s):

Ping Guo ◽

Weiwei Xu ◽

Shi Tang ◽

Binxia Cao ◽

Danna Wei ◽

...

Keyword(s):

Low Temperature ◽

Carbon Source ◽

Crude Oil ◽

Surface Activity ◽

Yellow Sea ◽

High Surface ◽

Sole Source ◽

The Yellow Sea ◽

Isolation And Characterization ◽

First Time

One cold-adapted strain, named Planococcus sp. XW-1, was isolated from the Yellow Sea. The strain can produce biosurfactant with petroleum as sole source of carbon at low temperature (4 °C). The biosurfactant was identified as glycolipid-type biosurfactant species by thin-layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). It reduced the surface tension of water to 26.8 mN/m with a critical micelle concentration measurement of 60 mg/L. The produced biosurfactant possesses high surface activity at wide ranges of temperature (−18–105 °C), pH values (2–12), and salt concentrations (1–18%). The biosurfactant exhibited higher surface activity and higher growth rate of cells with hexadecane and diesel as carbon source. The strain Planococcus sp. XW-1 was also effective in degrading crude oil, after 21 days of growth at 4 °C in medium with 1% crude oil and 1% (v/v) bacteria broth, 54% of crude oil was degraded. The results suggest that Planococcus sp. XW-1 is a promising candidate for use in the bioremediation of petroleum-contaminated seawater in the Yellow Sea during winter. This study reported for the first time that Planococcus isolated from the Yellow Sea can produce biosurfactant using petroleum as the sole carbon source at low temperature (4 °C), showing its ecological role in the remediation of marine petroleum pollution.

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Polarizing effect in natural polymer cellulose

Plasticheskie massy ◽

10.35164/0554-2901-2019-9-10-45-46 ◽

2019 ◽

pp. 45-46

Author(s):

N. N. Matveev ◽

V. V. Saushkin ◽

N. Yu. Evsikova ◽

N. S. Kamalova ◽

V. I. Lisitsyn

Keyword(s):

Temperature Field ◽

Low Temperature ◽

Natural Polymer ◽

Uniform Temperature ◽

Polarization Mechanism ◽

Uniform Temperature Field ◽

First Time

For the first time, a method based on the registration of polarization and depolarization currents arising in wood in a non-uniform temperature field was used to study the properties of cellulose. The purpose of the method used is to record the relaxation of bound charges with a change in the temperature of the sample under study. It is shown that the detected low-temperature transitions have a crystal-crystal polarization mechanism, and the natural polymer cellulose is an active dielectric.

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Low-temperature, inkjet printed p-type copper(i) iodide thin film transistors

Journal of Materials Chemistry C ◽

10.1039/c6tc03234f ◽

2016 ◽

Vol 4 (43) ◽

pp. 10309-10314 ◽

Cited By ~ 21

Author(s):

Chang-Ho Choi ◽

Jenna Y. Gorecki ◽

Zhen Fang ◽

Marshall Allen ◽

Shujie Li ◽

...

Keyword(s):

Thin Film ◽

Low Temperature ◽

Thin Film Transistors ◽

P Type ◽

First Time

Low temperature fabrication of printed p-type CuI TFTs was reported for the first time.

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Low-temperature and facile solution-processed two-dimensional TiS2 as an effective electron transport layer for UV-stable planar perovskite solar cells

Journal of Materials Chemistry A ◽

10.1039/c8ta01143e ◽

2018 ◽

Vol 6 (19) ◽

pp. 9132-9138 ◽

Cited By ~ 35

Author(s):

Guannan Yin ◽

Huan Zhao ◽

Jiangshan Feng ◽

Jie Sun ◽

Junqing Yan ◽

...

Keyword(s):

Solar Cells ◽

Electron Transport ◽

Low Temperature ◽

Perovskite Solar Cells ◽

Transport Layer ◽

Electron Transport Layer ◽

Two Dimensional ◽

Solution Processed ◽

Effective Electron ◽

First Time

In this paper, it is demonstrated that two-dimensional TiS2 nanosheets can be applied as an effective ETL in planar PSCs for the first time.

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Performance Improvement of RF Inductors Using LTCC Technology

Additional Conferences (Device Packaging HiTEC HiTEN & CICMT) ◽

10.4071/cicmt-2011-ta23 ◽

2011 ◽

Vol 2011 (CICMT) ◽

pp. 000054-000058 ◽

Cited By ~ 3

Author(s):

Goran Radosavljević ◽

Andrea Marić ◽

Walter Smetana ◽

Ljiljana Živanov

Keyword(s):

Experimental Data ◽

Low Temperature ◽

Design Optimization ◽

Quality Factor ◽

Performance Improvement ◽

Air Gap ◽

Frequency Range ◽

Operating Frequency Range ◽

Rf Inductors ◽

First Time

This paper presents for the first time a parallel comparison of the performance of RF inductors realized on different substrate configurations. Presented inductors are meander type structures fabricated in Low Temperature Co-fired Ceramic (LTCC) technology. Also, chosen material is never before implemented for inductor fabrication. The performance improvement is achieved by design optimization of different substrate configurations that incorporate placement of an air-gap beneath the inductor and/or introduction of an additional shielding layer on the top. Designed structures are characterized on the basis of simulation and experimental data, achieving good correlation between obtained results. Presented results show over 30 % increase in quality factor and widening of the operating frequency range by over 55 %.

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