scholarly journals A class III WRKY transcription factor in sugarcane was involved in biotic and abiotic stress responses

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Dongjiao Wang ◽  
Ling Wang ◽  
Weihua Su ◽  
Yongjuan Ren ◽  
Chuihuai You ◽  
...  
Keyword(s):  
Stress Responses ◽  
Transcriptional Activation ◽  
Abiotic Factors ◽  
Plant Stress ◽  
Bacterial Pathogen ◽  
Wrky Transcription Factor ◽  
Expression Level ◽  
Class Iii ◽  
Biotic And Abiotic Stress ◽  
Sporisorium Scitamineum

AbstractWRKY transcription factors play significant roles in plant stress responses. In this study, a class III WRKY gene ScWRKY5, was successfully isolated from sugarcane variety ROC22. The ScWRKY5 was a nucleus protein with transcriptional activation activity. The ScWRKY5 gene was constitutively expressed in all the sugarcane tissues, with the highest expression level in the stem epidermis and the lowest in the root. After inoculation with Sporisorium scitamineum for 1 d, the expression level of ScWRKY5 was significantly increased in two smut-resistant varieties (YZ01-1413 and LC05-136), while it was decreased in three smut-susceptible varieties (ROC22, YZ03-103, and FN40). Besides, the expression level of ScWRKY5 was increased by the plant hormones salicylic acid (SA) and abscisic acid (ABA), as well as the abiotic factors polyethylene glycol (PEG) and sodium chloride (NaCl). Transient overexpression of the ScWRKY5 gene enhanced the resistance of Nicotiana benthamiana to the tobacco bacterial pathogen Ralstonia solanacearum, however the transiently overexpressed N. benthamiana was more sensitive to the tobacco fungal pathogen Fusarium solani var. coeruleum. These results provide a reference for further research on the resistance function of sugarcane WRKY genes.

scholarly journals NAC TRANSCRIPTION FACTORS ROLE IN VARIOUS BIOTIC AND ABIOTIC STRESSES

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Hafsa Mansoor ◽  
Nadia Iqbal ◽  
Maryam Zain ◽  
Farah Deeba
Keyword(s):  
Transcription Factors ◽  
Abiotic Factors ◽  
Abiotic Stress Tolerance ◽  
Plant Stress ◽  
Stress Factors ◽  
Biotic And Abiotic Stress ◽  
Nac Transcription Factors ◽  
Dna Binding Domains ◽  
Binding Domains ◽  
Transcriptional Reprogramming

NAC transcription factors are considered as main family of transcriptional regulators in plants. NAC gene family members play significant contribution in regulating transcriptional reprogramming in plants related to plant stress response. These proteins possess highly conserved DNA binding domains and play a diverse functions in several plants. NAC gene is related to several stress factors including biotic and abiotic factors. NAC transcription factors controls several interrelated processes and their protein products can function as negative or positive regulators in many cellular processes. These regulatory functions are also controlled by NAC proteins such as auto and cross regulation. These regulatory proteins are regarded as a central regulator for the interaction of phyto hormones in various stress signaling pathways. This review highlights the role of NAC transcription factors in modulating gene expression and their role in various biotic and abiotic stress tolerance in plants.

scholarly journals A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shuangtao Li ◽  
Zhirong Wang ◽  
Fei Wang ◽  
Hongmei Lv ◽  
Meng Cao ◽  
...  
Keyword(s):  
Seed Germination ◽  
Osmotic Stress ◽  
Stress Responses ◽  
Transcriptional Activation ◽  
Stomatal Closure ◽  
Negative Regulator ◽  
Biotic And Abiotic Stress ◽  
Growth Activity ◽  
A Subunit ◽  
Antioxidant Enzymes Activities

Abstract Background TLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals. TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown. In this paper we characterized the biological function of cucumber (Cucumis sativus L.) Tubby-like protein 8 (CsTLP8) in Arabidopsis. Results In cucumber, the expression of the tubby-like protein CsTLP8 was induced by NaCl treatment, but reduced by PEG (Polyethylene Glycol) and ABA (Abscisic Acid) treatment. Subcellular localization and transcriptional activation activity analysis revealed that CsTLP8 possessed two characteristics of classical transcription factors: nuclear localization and trans-activation activity. Yeast two-hybrid assay revealed interactions of CsTLP8 with CsSKP1a and CsSKP1c, suggesting that CsTLP8 might function as a subunit of E3 ubiquitin ligase. The growth activity of yeast with ectopically expressed CsTLP8 was lower than the control under NaCl and mannitol treatments. Under osmotic and salt stresses, overexpression of CsTLP8 inhibited seed germination and the growth of Arabidopsis seedlings, increased the content of MDA (Malondialdehyde), and decreased the activities of SOD (Superoxide Dismutase), POD (Peroxidase) and CAT (Catalase) in Arabidopsis seedlings. Overexpression of CsTLP8 also increased the sensitivity to ABA during seed germination and ABA-mediated stomatal closure. Conclusion Under osmotic stress, CsTLP8 might inhibit seed germination and seedling growth by affecting antioxidant enzymes activities. CsTLP8 acts as a negative regulator in osmotic stress and its effects may be related to ABA.

scholarly journals Association of transcription factor WRKY56 gene from Populus simonii × P. nigra with salt tolerance in Arabidopsis thaliana

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7291 ◽  
Author(s):  
Lei Wang ◽  
Wenjing Yao ◽  
Yao Sun ◽  
Jiying Wang ◽  
Tingbo Jiang
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Germination Rate ◽  
Wrky Transcription Factor ◽  
Biotic And Abiotic Stress ◽  
Populus Simonii ◽  
Reading Frame

The WRKY transcription factor family is one of the largest groups of transcription factor in plants, playing important roles in growth, development, and biotic and abiotic stress responses. Many WRKY genes have been cloned from a variety of plant species and their functions have been analyzed. However, the studies on WRKY transcription factors in tree species under abiotic stress are still not well characterized. To understand the effects of the WRKY gene in response to abiotic stress, mRNA abundances of 102 WRKY genes in Populus simonii × P. nigra were identified by RNA sequencing under normal and salt stress conditions. The expression of 23 WRKY genes varied remarkably, in a tissue-specific manner, under salt stress. Since the WRKY56 was one of the genes significantly induced by NaCl treatment, its cDNA fragment containing an open reading frame from P. simonii × P. nigra was then cloned and transferred into Arabidopsis using the floral dip method. Under salt stress, the transgenic Arabidopsis over-expressed the WRKY56 gene, showing an increase in fresh weight, germination rate, proline content, and peroxidase and superoxide dismutase activity, when compared with the wild type. In contrast, transgenic Arabidopsis displayed a decrease in malondialdehyde content under salt stress. Overall, these results indicated that the WRKY56 gene played an important role in regulating salt tolerance in transgenic Arabidopsis.

scholarly journals Expression Characteristics and Functional Analysis of the ScWRKY3 Gene from Sugarcane

2018 ◽  
Vol 19 (12) ◽  
pp. 4059 ◽  
Author(s):  
Ling Wang ◽  
Feng Liu ◽  
Xu Zhang ◽  
Wenju Wang ◽  
Tingting Sun ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Functional Characterization ◽  
Bacterial Pathogen ◽  
Future Research ◽  
Sporisorium Scitamineum ◽  
Transcriptional Regulatory ◽  
Transient Overexpression ◽  
Hybrid Cultivar ◽  
Sugarcane Stem

The plant-specific WRKY transcriptional regulatory factors have been proven to play vital roles in plant growth, development, and responses to biotic and abiotic stresses. However, there are few studies on the WRKY gene family in sugarcane (Saccharum spp.). In the present study, the characterization of a new subgroup, IIc WRKY protein ScWRKY3, from a Saccharum hybrid cultivar is reported. The ScWRKY3 protein was localized in the nucleus of Nicotiana benthamiana leaves and showed no transcriptional activation activity and no toxic effects on the yeast strain Y2HGold. An interaction between ScWRKY3 and a reported sugarcane protein ScWRKY4, was confirmed in the nucleus. The ScWRKY3 gene had the highest expression level in sugarcane stem pith. The transcript of ScWRKY3 was stable in the smut-resistant Saccharum hybrid cultivar Yacheng05-179, while it was down-regulated in the smut-susceptible Saccharum hybrid cultivar ROC22 during inoculation with the smut pathogen (Sporisorium scitamineum) at 0–72 h. ScWRKY3 was remarkably up-regulated by sodium chloride (NaCl), polyethylene glycol (PEG), and plant hormone abscisic acid (ABA), but it was down-regulated by salicylic acid (SA) and methyl jasmonate (MeJA). Moreover, transient overexpression of the ScWRKY3 gene in N. benthamiana indicated a negative regulation during challenges with the fungal pathogen Fusarium solani var. coeruleum or the bacterial pathogen Ralstonia solanacearum in N. benthamiana. The findings of the present study should accelerate future research on the identification and functional characterization of the WRKY family in sugarcane.

Identification of a novel mitogen-activated protein kinase kinase gene (MKK2) in the oilseed rape Brassica campestris

Biologia ◽  
2014 ◽  
Vol 69 (11) ◽  
Author(s):  
Teng Zhang ◽  
Yuan Wang ◽  
Juan Wang ◽  
Xiao Xia ◽  
Ning Yang ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Stress Responses ◽  
Brassica Campestris ◽  
Plant Stress ◽  
Mitogen Activated Protein Kinase ◽  
Biotic And Abiotic Stress ◽  
Kinase Gene ◽  
Isolation And Characterization ◽  
Mapk Cascades ◽  
Mitogen Activated Protein

AbstractMitogen-activated protein kinase (MAPK) cascades participate in various processes, including plant growth and development as well as biotic and abiotic stress responses. MAPK kinases (MKKs), which link MPKs and MPKK kinases, are involved in MAPK cascades by mediating various plant stress responses. However, only a few MKKs from Brassica campestris (rape) have been functionally characterized. This study delivers the results from isolation and characterization of a novel gene, MKK2, from rape. Bioinformatics analysis revealed that the cDNA length of MKK2 is 1,344 bp with an open reading frame of 1,068 bp, which encodes a polypeptide containing 355 amino acids. The obtained MKK2 exhibited a predicted molecular mass of 39.3 kDa and an isoelectric point of 6.8. Quantitative real-time polymerase chain reaction analysis revealed that MKK2 expression can be induced by cold and salt. Western blot analysis revealed that MKK2 protein expression can be induced by cold, salt, and UV-B radiation. The MKK2 protein was localized in the nucleus. These results suggest that MKK2 is important for the regulation of cold- and salt-stress responses in plants.

scholarly journals Strawberry WRKY Transcription Factor WRKY50 Is Required for Resistance to Necrotrophic Fungal Pathogen Botrytis cinerea

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2377
Author(s):  
Chuangju Ma ◽  
Jinsong Xiong ◽  
Morong Liang ◽  
Xiaoyu Liu ◽  
Xiaodong Lai ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Botrytis Cinerea ◽  
Stress Responses ◽  
Protein Biosynthesis ◽  
Plant Stress ◽  
Wrky Transcription Factor ◽  
Antimicrobial Protein ◽  
Strawberry Fruit ◽  
Wrky Protein ◽  
Plant Stress Responses

WRKY protein is one of the largest plant-specific transcription factors that plays critical roles in plant stress responses, but few WRKY transcription factors have been functionally analyzed in strawberry. In this study, a Botrytis cinerea response WRKY gene, FvWRKY50, was isolated from the woodland strawberry. Expression analysis indicated that the transcript of FvWRKY50 was gradually decreased with fruit ripening, but was significantly induced by B. cinerea infection in mature strawberry fruit. Subcellular localization assay revealed that FvWRKY50 was localized in the nucleus. Several cis-elements related to pathogen responses were observed in the promoter region of FvWRKY50. Pathogen infection assay indicated that overexpression of FvWRKY50 in strawberry fruit significantly enhanced their resistance against B. cinerea, while the silencing of FvWRKY50 dramatically compromised their disease-resistant ability. The expression levels of several genes involved in jasmonic acid (JA) biosynthesis, signaling transduction, and antimicrobial protein biosynthesis were regulated to diverse extents in FvWRKY50 overexpressed and silenced fruit. Collectively, our study inferred that FvWRKY50 is a positive regulator that mediates resistance against B. cinerea through regulating some JA pathway and defense-related genes.

scholarly journals Relationship of Melatonin and Salicylic Acid in Biotic/Abiotic Plant Stress Responses

Agronomy ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 33 ◽  
Author(s):  
Josefa Hernández-Ruiz ◽  
Marino Arnao
Keyword(s):  
Salicylic Acid ◽  
Stress Responses ◽  
Plant Stress ◽  
Point Of View ◽  
Biotic And Abiotic Stress ◽  
Common Precursor ◽  
Relevant Role ◽  
Plant Stress Responses ◽  
Action Model ◽  
Relationship Of

Melatonin (N-acetyl-5-methoxytryptamine) was discovered in plants in 1995, while salicylic acid was the name given to the active ingredient of willow in 1838. From a physiological point of view, these two molecules present in plants have never been compared, even though they have a great number of similarities, as we shall see in this work. Both molecules have biosynthesis pathways that share a common precursor and both play a relevant role in the physiology of plants, especially in aspects related to biotic and abiotic stress. They have also been described as biostimulants of photosynthetic processes and productivity enhancers in agricultural crops. We review the coincident aspects of both molecules, and propose an action model, by which the relationship between these molecules and other agents and plant hormones can be studied.

scholarly journals Mechanisms and Functions of Long Non-Coding RNAs at Multiple Regulatory Levels

2019 ◽  
Vol 20 (22) ◽  
pp. 5573 ◽  
Author(s):  
Xiaopei Zhang ◽  
Wei Wang ◽  
Weidong Zhu ◽  
Jie Dong ◽  
Yingying Cheng ◽  
...  
Keyword(s):  
Chromatin Remodeling ◽  
Stress Responses ◽  
Transcriptional Activation ◽  
Rna Processing ◽  
Mrna Translation ◽  
Biological Processes ◽  
Biotic And Abiotic Stress ◽  
Plant Growth And Development ◽  
Regulate Gene Expression ◽  
Non Coding Rnas

Long non-coding (lnc) RNAs are non-coding RNAs longer than 200 nt. lncRNAs primarily interact with mRNA, DNA, protein, and miRNA and consequently regulate gene expression at the epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels in a variety of ways. They play important roles in biological processes such as chromatin remodeling, transcriptional activation, transcriptional interference, RNA processing, and mRNA translation. lncRNAs have important functions in plant growth and development; biotic and abiotic stress responses; and in regulation of cell differentiation, the cell cycle, and the occurrence of many diseases in humans and animals. In this review, we summarize the functions and mechanisms of lncRNAs in plants, humans, and animals at different regulatory levels.

scholarly journals Characterization of BRS1 Functions in Plant Stress Responses

2020 ◽  
Author(s):  
Dongzhi Zhang ◽  
Peng Zhao ◽  
Shengbao Xu
Keyword(s):  
Stress Responses ◽  
Redox Regulation ◽  
Plant Stress ◽  
Serine Carboxypeptidase ◽  
Biotic And Abiotic Stress ◽  
Brassinosteroid Signaling ◽  
Plant Stress Responses ◽  
Precise Role

Abstract Background: Brassinosteroid-insensitive 1 suppressor 1 (BRS1), is a serine carboxypeptidase that mediates brassinosteroid signaling and participates in multiple developmental processes in Arabidopsis. However, little is known about the precise role of BRS1 in this context. Results: In this study, we analyzed transcriptional and proteomic profiles of Arabidopsis seedlings overexpressing BRS1 and found that this gene is involved in both biotic and abiotic stress responses and redox regulation. Further proteomic evidence shows that BRS1 regulates cell redox by indirectly interacting with cytosolic NADP+-dependent isocitrate dehydrogenase (cICDH). We identified two novel splice products of BRS1, which might play important roles in development and stress responses in plants. Conclusions: This study highlights the role of BRS1 in plant redox regulation and stress responses, which extends our understanding of extracellular serine carboxypeptidases.

scholarly journals Arabidopsis thaliana GTS1 transcripts are activated by yeast extract

2021 ◽  
Vol 45 (2) ◽  
pp. 195-201
Author(s):  
Burcu Arıkan ◽  
Aslı Semercі ◽  
Ozgur Cakır ◽  
Kara Turgut
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Yeast Extract ◽  
Protein Interactions ◽  
Stress Responses ◽  
Ribosome Biogenesis ◽  
Plant Stress ◽  
Stress Factors ◽  
Biotic And Abiotic Stress ◽  
Wd40 Repeat

WD40 repeat-containing proteins participate in DNA-protein and protein-protein interactions and positively regulate plant stress responses. GTS1, known as a WD40 repeat-containing protein, works as a scaffold protein and is important in ribosome biogenesis and also biomass accumulation. In this study, we evaluated the GIGANTUS1 (GTS1) gene expression in response to biotic and abiotic stress factors in Arabidopsis thaliana plants. In addition, we grew and characterized A. thaliana gts1 mutant (T-DNA SALK_010647) in order to observe the effects of its absence on plants. According to our results, 100-200 mM abscisic acid (ABA) and 100-200 mM sodium chloride (NaCl) treatment did not cause any changes in GTS1 gene expression, while only 6 h of 1 g/l and 2 g/l yeast extract (YE) treatment negatively affected GTS1 expression in 10-day-old plant explants. After 10 and 30 days of YE treatment, GTS1 gene expression was upregulated, and as a consequence plant growth efficiency was reduced. We thus concluded that through the downregulation of GTS1 transcripts, we could obtain better growth and/or higher biomass, which seems to be a good option for agricultural recruitments.

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