scholarly journals Cannabis sativa L. may reduce aggressive behaviour towards humans in shelter dogs

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sara Corsetti ◽  
Simona Borruso ◽  
Livia Malandrucco ◽  
Valentina Spallucci ◽  
Laura Maragliano ◽  
...  
Keyword(s):  
Olive Oil ◽  
Aggressive Behaviour ◽  
Cannabis Sativa ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Treated Group ◽  
Control Group ◽  
Focal Animal ◽  
Shelter Dogs ◽  
The Difference

AbstractAmong the phytocomplex components of Cannabis sativa L., cannabidiol (CBD) has a recognised therapeutic effect on chronic pain. Little is known about the veterinary use of CBD in dogs. Even less is known on the effects of CBD on dog behaviour, especially in shelters. The purpose of this study was to determine if CBD affects stress related behaviour in shelter dogs. The sample consisted of 24 dogs divided into two groups that were created by assigning the dogs alternately: 12 dogs were assigned to the treatment group and 12 to the control group. Extra virgin olive oil, titrated to 5% in CBD was given to treated group; the placebo consisted of olive oil only, dispensed daily for 45 days. Behavioural data were collected using the ‘focal animal’ sampling method with ‘all occurrences’ and ‘1/0’ methods for 3 h: before (T0), after 15 days (T1), after 45 days of treatment (T2) and after 15 days from the end of the treatment (T3). Treated dogs showed reduced aggressive behaviour toward humans following the treatment (Friedman Test: χ2 = 13.300; df = 3; N = 12; p = .004; adj. sig. p = 0.027), but the difference in the decrease of aggressive behaviour between the two groups was not significant (Mann–Whitney U test, T2–T0: Z = − 1.81; N = 24; p = 0.078). Other behaviours indicative of stress, such as displacing activities and stereotypes, did not decrease. Despite some non-significant results, our findings suggest that it is worth doing more research to further investigate the effect of CBD on dog behaviour; this would be certainly valuable because the potential for improving the welfare of dogs in shelters is priceless.

scholarly journals Extra Virgin Olive Oil Modulates Vasodilator Enzyme Level by Repairing Angiogenesis Function in Rat Model of Preeclampsia

2020 ◽  
Author(s):  
Yulia Silvani ◽  
Agnestia Naning Dian Lovita ◽  
Afniari Maharani ◽  
I Wayan Arsana Wiyasa ◽  
Hidayat Sujuti ◽  
...  
Keyword(s):  
Olive Oil ◽  
Rat Model ◽  
Enzyme Level ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Pregnant Rats ◽  
Vegf Level

Objective: This study aimed to determine the effect of Extra Virgin Olive Oil (EVOO) on vasodilator enzyme by repairing angiogenic function in rat model of preeclampsia. Materials and methods: This research consisted of five groups; negative control (normal pregnant rats) group, positive control (preeclampsia rat model) group, preeclampsia rat model groups given EVOO in 3 different doses (0.5 ml/day, 1 ml/day, and 2 ml/day, respectively). Blood pressure measurements were carried out on day 12, 15, and 19 of pregnancy. After the rats were sacrificed, the placentas were collected to determine endothelial Nitric Oxide Synthase (eNOS) level of maternal plasma to determine soluble Fms-like Tyrosine Kinase 1 (sFlt-1) and Vascular Endothelial Growth Factor (VEGF) level. Results: There were significant higher sFlt-1 level (p < 0.001), lower VEGF level (p = 0.009), and lower eNOS level (p = 0.034) between negative and positive control groups. After EVOO administration, sFlt-1 level was lower in dose 1 and 2 groups but higher in dose 3 group in accordance with VEGF and eNOS levels that were increasing both in dose 1 and dose 2 groups but decreasing in dose 3. There were significant differences between positive control and dose 1 (p = 0.015) and dose 2 (p = 0.001) in sFlt-1 level. None of all dose groups were statistically different with positive control group in VEGF level (dose 1 p = 0.601; dose 2 p = 0.297; dose 3 p = 0.805). eNOS levels of all dose groups were statistically different from that of the positive control group (dose 1 p = 0.014; dose 2 p = 0.001; dose 3 p = 0.024). Conclusion: Administration of EVOO modulates eNOS as vasodilator enzyme by repairing the angiogenic function indicated by decreased sFlt-1 level and increased VEGF in rat model of preeclampsia.

scholarly journals Extra Virgin Olive Oil meningkatkan ekpresi reseptor estrogen α dan ketebalan Endometrium Rattus Norvegicus yang dipapar Rhodamin B

2018 ◽  
Vol 5 (3) ◽  
pp. 201-206
Author(s):  
Ita Noviasari ◽  
I Wayan Arsana Wiyasa ◽  
Hidayat Sujuti ◽  
Eviana Norahmawati
Keyword(s):  
Oxidative Stress ◽  
Estrogen Receptors ◽  
Olive Oil ◽  
Rattus Norvegicus ◽  
Endometrial Thickness ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
The Body ◽  
Control Group ◽  
Rhodamin B

Abstract: Rhodamin B is harmful to the body because it is proven can increase the oxidative stress on various reproductive chain. Rhodamin B contains compounds of chlorine (Cl), is dangerous and halogen compounds are reactive. Damage to the hypothalamus will interfere with the secretion of FSH and LH cause the follicle ripening so distracted that result in a decrease in the hormone estrogen. The hormone estrogen plays an important role in the menstrual cycle and proliferation of the endometrium. This research aimed to prove the effect of Extra Virgin Olive Oil (EVOO) with the expression of estrogen receptors α and endometrial thickness of Rattus norvegicus exposed by rhodamin B. The design used true experimental with an approach of post test only control group design. The research used white rat (Rattus norvegicus wistar strain) as the sample. The results showed that EVOO could become an alternative to prevent oxidative stress due to exposure of rhodamin B.Keywords: Rhodamin B, Extra Virgin Olive Oil (EVOO), Estrogen receptors α, the thickness of the Endometrium

scholarly journals Extra Virgin Olive Oil Protects the Testis and Blood from the Toxicity of Paracetamol (Overdose) in Adult Male Rats

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1042
Author(s):  
Latifa Ishaq Khayyat
Keyword(s):  
Olive Oil ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Paracetamol Overdose ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Ultrastructural Studies ◽  
Group 4 ◽  
Group 3

Extra virgin olive oil (EVOO) is important in people’s daily diets. Paracetamol is a widely used analgesic and antipyretic drug. The aim of this study is to investigate the protective effect of EVOO against hematotoxicity and testicular toxicity induced by paracetamol overdose in rats. Forty rats were divided into four groups. Group 1 rats were given water (control), Group 2 rats were given oral EVOO daily (2 mL/kg b.wt.), Group 3 rats were given oral paracetamol daily (650 mg/kg b.wt.), and Group 4 rats were given paracetamol and EVOO daily. After 15 days, blood and testis samples were collected for biochemical, histological, and ultrastructural studies. The results show that paracetamol decreased the PCV, Hb, and RBC counts relative to the control, and significantly increased the WBC counts and stab cells in Group 3. A significant decrease in blood testosterone was found in Group 3 compared to the control, while a significant increase in testosterone levels was observed in Group 4 compared to Group 3. Light and electron microscopy showed disorganized seminiferous tubules in Group 3. The testis in Group 4 appeared in normal shape. In conclusion, the results indicate that EVOO protects the testis and blood from paracetamol toxicity and may also increase fertility in male rats.

scholarly journals Efektifitas Pemberian Ekstra Virgin Minyak Zaitun Dan Ekstrak Kulit Manggis (Mastin) Terhadap Kadar Kolesterol Tikus Putih Starin Wistar Jantan Yang Diinduksi Hiperlipedemia

2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Yeni Tutu Rohimah ◽  
Sri Lestari Dwi Astuti
Keyword(s):  
Olive Oil ◽  
Total Cholesterol ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Positive Control ◽  
Control Group ◽  
High Fat ◽  
Mangosteen Peel ◽  
Male Wistar Rats ◽  
Peel Extract

Abstract : Extra Virgin Olive Oil, Mangosteen Peel Extract, Serum Lipid Levels. Cardiovascular disease was the number one cause of death, risk factors of cardiovascular disease were divided into the risk factors that can’t be modified and the factors that can be modified. Some of the plants that have therapeutic benefit are olive oil and mangosteen peel extract. Olive oil is a useful food because of its content is rich in monounsaturated fatty acids (MUFA). Mangosteen peel extract has properties as antilipemic that can increase the activity of Peroxisome proliferator-Activated Receptors (PPAR). This research had a purpose to examine the effectiveness between Extra Virgin Olive Oil and the mangosteen peel extract against cholesterol levels. The methode of this research was laboratory experimental with post test only control group design. Thirty eight male wistar rats were divided into four groups. The negative control (K1) was fed with standard food for 38 days. The positive control (K2) was fed with high fat food for 38 days. The first treatment group (P1) was fed with high fat food for 38 days then it was fed with standard food and Extra Virgin Olive Oil 0,5 gr/day for 28 days. The second treatment group (P2) was fed with high fat food for 38 days then it was fed with standard food and mangosteen peel extract 0,0125 gr/days for 28 days. Afterwards, all of the groups total cholesterol and trigliserida were examined. The data analysis used One Way Anova then it tested by post-hok (p > 0,005). The result for K2 were 336,9 ± 43,76 trigliserida and 263,3 ± 12,9 total cholesterol, K1 were 138,2 ±12,7 trigliserida and 76,3 ± 8,2 total cholesterol, P1 were 325,02 ± 38,2 trigliserida and 73,6 ± 17,8 total cholesterol, P2 were 328 ± 48,14 trigliserida and 66, 5 ± 13,7 total cholesterol. The analysis result between positive control group with Extra Virgin Olive Oil and the mangosteen peel extract for total cholesterol was p < 0,05 while for trigliserida was p > 0,05. The ratio Extra Virgin Olive Oil and the mangosteen peel extract was p > 0,05. Extra Virgin Olive Oil and the mangosteen peel extract has the same effectiveness to reduce cholesterol on a male wistar rats that were induced by hyperlipidemia.

scholarly journals Pengaruh pemberian Extra Virgin Olive Oil terhadap jumlah Sel Granulosa dan kadar 17 β Estradiol tikus yang di papar Rhodamin B

2018 ◽  
Vol 5 (3) ◽  
pp. 241-248
Author(s):  
Fera Yuli Setiyaningsih ◽  
Pande Made Dwi Jayasa ◽  
Hidayat Sujuti ◽  
Diah Prabawati Retnani
Keyword(s):  
Treatment Group ◽  
Olive Oil ◽  
Granulosa Cells ◽  
Rhodamine B ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Positive Control ◽  
Control Group ◽  
17Β Estradiol ◽  
Rhodamin B

Abstract: The use of dyes in food today is very apprehensive. Many foods contain dyes that are prohibited to be used in food. The addition of the dye will have an impact on health. The aim of this research was to know the effectiveness of the administration of extra virgin olive oil to the number of granulosa cell and 17β estradiol level of Rats exposed by rhodamin B. The design of this study was true experimental with post test approach of group design control. The subject of the study was 30 white mice Rattus novergicus in the age of 12 weeks weight average 220 grams and in healthy condition. Rats were divided into 5 groups. Control group without treatment, positive control treatment with rhodamine B 18 mg / 200gr. Treatment 1 administration of rhodamine B 18 mg / 200gr and EVOO (Extra Virgin Olive Oil) 1.5 ml / KgBB, treatment 2 rhodamine B 18 mg / 200gr and EVOO 3 ml / KgBB, treatment 3 rhodamine B 18 mg / 200gr and EVOO 4,5 ml / KgBB. The number of granulosa cells was determined by HE staining, the level of 17β estradiol with ELISA. The data was analyzed by Kruskal Wallis test, Spearman Rank and Simple Linear Regression with degree of significance α <0.05. The average number of granulosa cells and the lowest levels of 17β estradiol were found in the positive control group and the highest average was in the treatment group 3. There was one dose that could significantly increase granulosa cells and the beta estradiol levels compared with the treatment group the other with niali α <0.05. The administration of EVOO affected the number of granulosa cells and the levels of 17β estradiol in rats exposed by rhodamine B.Keyword: granulosa cells, estradiol, rhodamin B, oxidative stress

scholarly journals Efektifitas Pemberian Minyak Zaitun Ekstra Virgin Terhadap Kadar Glukosa Darah Tikus Wistar Jantan Dengan Induksi Hiperglikemia

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Sri Lestari Dwi Astuti ◽  
Yeni Tutu Rohimah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Olive Oil ◽  
Glucose Level ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Standard Diet

Background: The global population of diabetes mellitus tends to increase every year. This is related to the increased of population numbers, lifestyle, increased prevalence of obesity and less physical activity (Smeltzer & Bare, 2002). Olive oil is a functional diet because it is rich of monounsaturated fatty acids (MUFA), especially oleic acid (70% - 80%), contains antioxidants and polyphenol compounds. Polyphenols are α-amylase enzyme inhibitors that have function in the breakdown of carbohydrates, so that the level of blood glucose in hyperglycemia can be lowered (Astrawan made, Wresdiyati Tutik, Nasution Nurlayla Amas, 2015). The goal of this study is to examine the effectiveness of extra virgin olive oil to blood glucose level. Method:This study used experimental laboratory study with post test only control group design. The independent variable was the extra virgin olive oil, while the dependent variable was the blood glucose level. The samples were 32 healthy 8 weeks old white male wistar strain rats with the weigh between 180 mg - 200 mg. It divided into 3 groups, the negative control group was fed with standard diet, the positive control group was fed with standard diet and 1 ml fructose for 4 weeks, the treatment group was fed with standard diet, 1 ml fructose and 0.5 gr extra virgin olive oil for 4 weeks, on the fifth week the fructose was increased into 4 ml/day for the next 4 weeks, on the 52nd day the blood glucose level was checked after it was fasted for 6 hours. The data analysis used t-test and Mann Withney test with α = 0.05. Result: The blood glucose level average of the positive control group was 309.57 ± 120.12, the blood glucose level average of the negative control group was 111.85 ± 10.57, the blood glucose level average of the treatment group was 115.12 ± 12.25. Conclusion: Giving the extra virgin olive oil can effectively lower the blood glucose level on the male wistar rats.

scholarly journals The Effect of Extra Virgin Olive Oil on LOX-1 and COX-2 in High Fat Diet Rats

2016 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Ibrahem Omran Lakhder ◽  
Lisyani Suromo ◽  
Kis Djamiatun
Keyword(s):  
Olive Oil ◽  
High Fat Diet ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Serum Levels ◽  
Control Group ◽  
High Fat ◽  
Oil Extract ◽  
Cox 2 ◽  
Anti Inflammatory

Background: High fat diet is a diet containing large amounts of fat consistently, the increase dietary fat and cholesterol which have a key role in growing health problems. Extra virgin olive oil associated with prevention of LDL oxidation, beneficial changes in lipid ratios and low risk for CHD.Objective: to determine the anti-inflammatory effect  of extra virgin olive oil extract to  levels of COX-2 and LOX-1 in the blood in rats induced by high fat dietMethods: This research is an experimental study that used randomized posttest only control group design. 30 Wistar rats which were divided into five groups:  group of control (-) which received  normal diet and group of control (+) which received  high fat diet without  EVOO treatment and three high fat diet groups treated by EVOO 1 mL/kg/day, 2 mL/kg/day and 3 mL/kg/day orally for 2 months. The blood was collected from eyes rats and serum separation by centrifuge. COX-2, LOX-1 concentration was measured by the enzyme linked immunosorbent assay (ELISA).Results: The result of this study showed higher COX-2 concentrations in groups treated with EVOO than control group. COX-2 serum levels of negative control where significantly lower than those of rats treated with 2 ml/kg/day (p = 0.047) and 3 ml/kg/day EVOO (p = 0.014). The COX-2 serum levels of group received 1 ml/kg/day were significantly lower than those of rats received 3 ml/kg/day EVOO (p = 0.027). And showed  not significantly deferent among all groups (p = 0.570).Conclusions: The conclusion of this study has showed that extra virgin olive oil extract might have minor anti inflammatory and antioxidant effect in rats.

scholarly journals Effect of Physical Activity, Nutritional Education, and Consumption of Extra Virgin Olive Oil on Lipid, Physiological, and Anthropometric Profiles in a Pediatric Population

2015 ◽  
Vol 12 (9) ◽  
pp. 1245-1252 ◽  
Author(s):  
José Joaquín Muros ◽  
Mikel Zabala ◽  
María Jesús Oliveras-López ◽  
Paula Rodríguez Bouzas ◽  
Emily Knox ◽  
...  
Keyword(s):  
Physical Activity ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Intervention Group ◽  
Extra Virgin Olive Oil ◽  
Control Group ◽  
Fat Percentage ◽  
Nutritional Education ◽  
Health Related ◽  
Combined Intervention

Background:The aim of this study was to determine the effects of nutritional education and vigorous physical activity on health-related parameters.Methods:The sample group consisted of 134 children from 5 rurally located schools. Participants were divided between 5 different experimental groups: control group (CG), physical activity group (PA), nutritional education group (NE), combined intervention group (PA+NE), and a combined intervention group with additional substitution of normally used oil for extra virgin olive oil (EVOO; PA+NE+EVOO). The intervention consisted of 60 minute sessions of physical activity held twice a week as well as nutritional education sessions held over 6 months.Results:Students in the groups receiving physical activity reduced their fat percentage and increased their muscle mass post intervention. At posttest the lipid profile improved in all intervention groups. The proportion of macronutrients and dietary cholesterol improved in the groups receiving nutritional education. The posttest comparison showed significantly lower fat percentage, sum of skinfolds and waist circumference in NE relative to CG and PA relative to CG. Diastolic blood pressure and glycaemia were significantly lower in PA+NE+EVOO relative to CG.Conclusion:A school-based program consisting of nutritional education or nutritional education plus a physical activity program showed a positive effect on health-related parameters in children.

scholarly journals The Effect of Extra Virgin Olive Oil on Low-Density Lipoprotein Levels in Productive Age Obesity Women in Kendari

2021 ◽  
pp. 59-65
Author(s):  
Arimaswati ◽  
Alfath Akbar J. Dundu ◽  
Pranita Aritrina
Keyword(s):  
Fatty Acids ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
The Body ◽  
Control Group ◽  
Antioxidant Compounds ◽  
P Value ◽  
Obese Women ◽  
Childbearing Age

Obesity is one of the burdens of nutritional problems in women of childbearing age due to fat accumulation (adiposity) in the body, thereby increasing the risk of health problems. Obese subjects tend to have high LDL levels, so consuming olive oil every day for one week will experience a decrease in LDL cholesterol and increase antioxidant compounds. Olive oil used in the medical world is a type of extra virgin olive oil (EVOO) that contains monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), omega 3, omega 6, oleic acid (omega 9), vitamin E, vitamin K, palmitic acid, pigments, phenolic, squalene. This study was a quasi-experimental study with a pretest-posttest approach without a control group. The independent variable was 30 ml extra virgin olive oil (EVOO) for 21 days in obese women of productive age in Kendari. The results of this study obtained a p-value of 0.112. Extra virgin olive oil has no effect on LDL levels in obese women of productive age in Kendari.

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