scholarly journals Optimization of OPEFB lignocellulose transformation process through ionic liquid [TEA][HSO4] based pretreatment

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Muhammad Nurdin ◽  
Haznan Abimanyu ◽  
Hadijah Putriani ◽  
L. O. M. Idal Setiawan ◽  
Maulidiyah Maulidiyah ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Lignin Content ◽  
Recovery Process ◽  
Decomposition Temperature ◽  
Transformation Process ◽  
Ir Absorption ◽  
Cellulose Content ◽  
Empty Fruit Bunches ◽  
H Nmr ◽  
Temperature Optimization

AbstractResearch on the transformation of Oil Palm Empty Fruit Bunches (OPEFB) through pretreatment process using ionic liquid triethylammonium hydrogen sulphate (IL [TEA][HSO4]) was completed. The stages of the transformation process carried out were the synthesis of IL with the one-spot method, optimization of IL composition and pretreatment temperature, and IL recovery. The success of the IL synthesis stage was analyzed by FTIR, H-NMR and TGA. Based on the results obtained, it showed that IL [TEA][HSO4] was successfully synthesized. This was indicated by the presence of IR absorption at 1/λ = 2814.97 cm−1, 1401.07 cm−1, 1233.30 cm−1 and 847.92 cm−1 which were functional groups for NH, CH3, CN and SO2, respectively. These results were supported by H-NMR data at δ (ppm) = 1.217–1.236 (N–CH2–CH3), 3.005–3.023 (–H), 3.427–3.445 (N–H+) and 3.867 (N+H3). The TGA results showed that the melting point and decomposition temperature of the IL were 49 °C and 274.3 °C, respectively. Based on pretreatment optimization, it showed that the best IL composition for cellulose production was 85 wt%. Meanwhile, temperature optimization showed that the best temperature was 120 °C. In these two optimum conditions, the cellulose content was obtained at 45.84 wt%. Testing of IL [TEA][HSO4] recovery performance for reuse has shown promising results. During the pretreatment process, IL [TEA][HSO4] recovery effectively increased the cellulose content of OPEFB to 29.13 wt% and decreased the lignin content to 32.57%. The success of the recovery process is indicated by the increasing density properties of IL [TEA][HSO4]. This increase occurs when using a temperature of 80–100 °C. The overall conditions obtained from this work suggest that IL [TEA][HSO4] was effective during the transformation process of OPEFB into cellulose. This shows the potential of IL [TEA][HSO4] in the future in the renewable energy sector.

scholarly journals Synthesis, pretreatment, and recovery of [TEA][HSO4] ionic liquid for OPEFB lignocellulosic transformation

2020 ◽  
Author(s):  
Muhammad Nurdin ◽  
Haznan Abimanyu ◽  
Hadijah Putriani ◽  
L.O.M. Setiawan ◽  
Maulidiyah Maulidiyah ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Lignin Content ◽  
Decomposition Temperature ◽  
Biomass Pretreatment ◽  
Chemical Components ◽  
One Pot ◽  
Empty Fruit Bunches ◽  
H Nmr ◽  
The One ◽  
Chemical Groups

Abstract In recent years, various studies have been utilized lignocellulosic biomass from oil palm empty fruit bunches (OPEFB) to be converted into bioethanol fuels. In this study, we present the techno-economical preparation for biomass pretreatment based on triethylamine hydrogen sulfate ([TEA][HSO4]) ionic liquid. Synthesis, pretreatment, and recovery of [TEA][HSO4] ionic liquid have been carried out via the one-pot method. Based on these results, the synthesized [TEA][HSO4] has been characterized using IR spectroscopy showing the appearance chemical groups such as H, CH3, CN, and SO2. In addition, H-NMR spectroscopy was presenced the CH3CH2- structure towards low ppm. Thermal stability has also demonstrated unique physical properties of ionic liquid (IL) with a melting point of 49°C and a decomposition temperature of 274.3°C. The effectiveness to produce the chemical components shows that the useful use of [TEA][HSO4] was successfully synthesized with an optimum composition of 83% resulting in chemical levels of 45.84% (cellulose), 5.00% (hemicellulose), and 34.40% (lignin). The recovered [TEA][HSO4] with a composition of 90.90% was effective in reducing the lignin content about 80%. These results also depend heavily on the temperature and separation techniques applied during the pretreatment process.

scholarly journals HIDROLISIS HASIL DELIGNIFIKASI TANDAN KOSONG KELAPA SAWIT DALAM SISTEM CAIRAN IONIK CHOLINE CHLORIDE

2016 ◽  
Vol 5 (1) ◽  
pp. 27-33
Author(s):  
Gendish Yoricya ◽  
Shinta Aisyah Putri Dalimunthe ◽  
Renita Manurung ◽  
Nimpan Bangun
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Reaction Time ◽  
Choline Chloride ◽  
Liquid System ◽  
Cooking Time ◽  
Raw Material ◽  
Cellulose Content ◽  
Empty Fruit Bunches ◽  
Hydrolysis Conditions

Palm Empty Fruit Bunches (TKKS) was the waste which has a fairly high content of lignocelluloses. Meanwhile, TKKS has not been utilize optimally. With a cellulose content of 45%-50%, TKKS then potentially be used as raw material for bioethanol. In the process of production bioethanol, delignification of lignocellulose the first phase was conducted to dissolve ligament between cellulose, hemicellulose and lignin. In this research, delignification process was carried out using NaOH in the ionic liquid system and without ionic liquids. The purpose of this research was to find out the highest content of cellulose which contained in the TKKS and to determine the hydrolysis of delignification results on palm empty fruit bunches and the best hydrolysis conditions was obtained at the hydrolysis process in the choline chloride ionic liquid system. Delignification process were performed using ionic liquids choline chloride (ChCl) in variety of cooking time with amount different ChCl. This research used TKKS powder cooked at a temperature 130 °C with a variety of cooking time 30, 60, and 90 minutes and the variation of ChCl 10%, 15% and 20% weight of TKKS. Delignification research results used ChCl obtained highest content of cellulose was 40,33%, hemicellulose 20,28%, and lignin 3,62% in cooking treatment 90 minutes and 15% ChCl. While delignification without ChCl obtained highest content of cellulose is 24,98%, hemicellulose 8,25%, and lignin 18,99% in cooking treatment 90 minutes. Delignification process using ChCl be able increase the degree of delignification as big as 61,45%. In the hydrolisis process, the main raw material used cellulose of delignification TKKS result, choline chloride, sulfatl acid, and distilled water. The hydrolysis stage in this research was carried out at temperature 105 0C, catalyst (H2SO4) 10% (w / w) cellulose, ChCl 10%, 15%, and 20% (w / w) cellulose and it was stirred at constant speed 120 rpm with reaction time of 30, 60 and 90 minutes. The result in the hydrolysis stage using ionic liquid obtained glucose. LUFF method analysis showed the maximum result of glucose 37.96% with the best conditions in reaction time 90 minutes and the amount of choline chloride 20%.

scholarly journals Simultaneous Pretreatment Process on The Isolation of Cellulose Mycrocrystalline from Oil Palm empty Fruit Bunches

REAKTOR ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 174-182
Author(s):  
Yulianti Sampora ◽  
Yenni Apriliany Devy ◽  
Dewi Sondari ◽  
Athanasia Amanda Septevani
Keyword(s):  
Oil Palm ◽  
Lignin Content ◽  
Morphological Changes ◽  
Sem Analysis ◽  
Cellulose Content ◽  
Simultaneous Reduction ◽  
Bleaching Process ◽  
Good Thermal Stability ◽  
Empty Fruit Bunches ◽  
Simultaneous Process

This research was aimed to evaluate the isolation of cellulose derived from empty fruit bunches (EFB), especially to increase the purity of cellulose content by subsequent pretreatment process involving delignification using NaOH followed by bleaching process using hydrogen peroxide (H2O2). The result showed that the cellulose content of the raw EFB content at 37.6% ± 0.3 w/w increased to 89.2% ± 0.4 w/w after the simultaneous pretreatment process. Moreover, after the simultaneous of bleaching process using H2O2, the lignin content can be reduced up to 3.1% ± 0.1 w/w which similar to cellulose standard (2.7% ± w/w). The utilization of simultaneous pretreatment process can also reduce the hemicellulose content from 23.9% ± 0.3 w/w (raw EFB) to 7.7% ± 0.2 w/w even lower than the cellulose standard (13.1% w/w). FTIR spectrum showed that the peak absorption of cellulose increased significantly upon the simultaneous process of delignification-bleaching. Morphological changes of the raw EFB compared to pre-treated EFB as a note by SEM analysis showed the significant transformation from the larger size and tightly bundles of micro-fibril cellulose into smaller size and individually separated micro-fibrils. Further through the simultaneous process exhibited yield at 80-85%, high crystallinity (70-80%), and good thermal stability in terms of Tonset at about 230-290 °C. From these results, it can be concluded that the subsequent pretreatment process involving NaOH delignification and H2O2 bleaching processes more likely offer a high selectivity of cellulose microcrystalline from lignocellulosic biomass material by simultaneous reduction of the hemicellulose and lignin content without disrupting the structure of cellulose.Keywords: bleaching; cellulose; delignified; microcrystalline cellulose; oil palm empty fruit bunches

scholarly journals Characteristic of oil palm empty fruit bunch pretreated with Pleurotus floridanus (Pretreatment biologi tandan kosong kelapa sawit menggunakan Pleurotus floridanus)

2017 ◽  
Vol 85 (2) ◽  
Author(s):  
. ISROI
Keyword(s):  
Oil Palm ◽  
Lignin Degradation ◽  
Lignin Content ◽  
White Rot Fungi ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Cellulose Content ◽  
Biological Pretreatment ◽  
Empty Fruit Bunches ◽  
Pleurotus Floridanus

Pleurotus floridanus have ability on lignin degradation by producing ligninolytic enzyme and prefer to degrade lignin than carbohydrate (hemicellulose and cellulose). Oil palm empty fruit bunches has been pretreated using P. floridanus.  Addition of cation (Cu2+) on biological pretreatment reduced lignin content and increased digestibility of the empty fruit bunches. P. floridanus reduce lignin and hemicellulose content from 23.9% to 10.1% and from 20.8% to 16.9%, respectively. P. floridanus did not degrade cellulose. Cellulose content of empty fruit bunches increase from 40.4% to 51.7%. Crystallinity of empty fruit bunches reduced after biological pretreatment. Crystallinity presented as LOI (lateral order index) of un-treated and biological pretreated oil palm empty fruit bunches are 2.08 and 1.44. Digestibility of the empty fruit bunches increased from 17.2% to 60.3% by biological pretreatment.[Key words:  biological pretreatment, oil palm empty fruit bunches, Pleurotus floridanus, biofuel, white-rot fungi, lignocellulose]AbstrakPleurotus floridanus memiliki kemampuan untuk mendegradasi lignin dengan memproduksi enzim ligninolitik dan lebih memilih untuk mendegradasi lignin daripada karbohidrat (hemiselulosa dan selulosa). Kemampuan unik P. floridanus ini dimanfaatkan dalam pretreatment biologi tandan kosong kelapa sawit. Penambahan kation (Cu2+) pada pretreatment biologi menurunkan kandungan lignin dan meningkatkan digestibiliti tandan kosong kelapa sawit. Perlakuan P. floridanus mengurangi kandungan lignin dan hemiselulosa dari 23,9% menjadi 10,1% dan dari 20,8% menjadi 16,9%. Perlakuan P. floridanus tidak menurunkan kandungan selulosa. Kandungan selulosa tandan kosong kelapa sawit meningkat dari 40,4% menjadi 51,7%. Kristalinitas tandan kosong menurun setelah pretreatment biologi. Kristalinitas yang dinyatakan dalam LOI (LOI, Lateral Order Index) adalah 2,08 untuk tandan kosong tanpa pretreatment biologi dan 1,44 untuk tandan kosong dengan pretreatment biologi. Digestibiliti itandan kosong meningkat dari 17,2% menjadi 60,3%.[Kata kunci: Pretreatment biologi, tandan kosong kelapa sawit, jamur pelapuk putih, lignoselulosa, Pleurotus floridanus]

scholarly journals Analisis Karakteristik Pulp Campuran Tandan Kosong Kelapa Sawit dan Pelepah Pisang dengan Pelarut NaOH

2021 ◽  
Vol 1 (10) ◽  
pp. 389-393
Author(s):  
Sari Rizky Amelia ◽  
Muhammad Yerizam ◽  
Erwana Dewi
Keyword(s):  
Raw Materials ◽  
Lignin Content ◽  
Tensile Modulus ◽  
Physicochemical Characteristics ◽  
Cooking Time ◽  
Cellulose Content ◽  
Optimum Conditions ◽  
Empty Fruit Bunches ◽  
Naoh Solution ◽  
High Cellulose

Tandan kosong kelapa sawit (TKKS) merupakan limbah padatan yang dihasilkan dari proses pembuatan minyak kelapa sawit yang selulosa (45,95%), kadar abu (1,23%), hemiselulosa (22,84%), kadar air (3,74%) dan lignin (16,49%). Pelepah pisang mempunyai kandungan selulosa yang tinggi akan tetapi belum dimanfaatkan secara optimal yang memiliki kandungan densitas ( 1,35 gr/cm3), selulosa (63 -64 %), hemiselulosa (20 %), lignin (5%), kekuatan Tarik rata-rata (600 Mpa), modulus tarik rata-rata (17,85 Gpa), pertambahan panjang (3,36 %), diameter serat (5,8 µm), serta panjang serat (30,9240 cm). Namun bahan-bahan ini mempunyai karakteristik dan sifat fisika kimia yang berbeda-beda. Karakteristik menjadikan perlunya analisa agar menghasilkan pulp yang diinginkan berada pada kondisi optimal. Beberapa parameter yang diukur di antaranya, konsentrasi larutan NaOH dengan variasi 7% dan 9%, dengan variasi bahan baku 40-60% serta waktu pemasakan dengan variasi waktu 75, 90, 105, 120 dan 135 menit. Sehingga pada penelitian ini didapatkan kondisi optimumnya yakni pada rasio 40:60, dengan konsentrasi 9% dan waktu pemasakan selama 120 menit didapatkan nilai kadar lignin 11,21% dan kadar selulosa 68,94%. Oil palm empty fruit bunches or TKKS are solid wastes produced from the process of making palm oil which are cellulose (45.95%), ash content (1.23%), hemicellulose (22.84%), water content (3.74 %) and lignin (16.49%). Banana midrib has a high cellulose content but has not been used optimally which contains density (1.35 g/cm3), cellulose (63 -64 %), hemicellulose (20 %), lignin (5%), average tensile strength. average (600 Mpa), average tensile modulus (17.85 Gpa), increase in length (3.36%), fiber diameter (5.8 m), and fiber length (30,9240 cm). However, these materials have different physicochemical characteristics and properties. The characteristics make the need for analysis in order to produce the desired pulp in optimal conditions. Several parameters were measured including the concentration of NaOH solution with variations of 7% and 9%, with variations of raw materials 40-60% and cooking time with variations of 75, 90, 105, 120 and 135 minutes. So that in this study the optimum conditions were obtained, namely at a ratio of 40:60, with a concentration of 9% and cooking time for 120 minutes, the lignin content was 11.21% and the cellulose content was 68.94%.

Analysis of Sound Reduction and Strong Drag Composite Concrete Fibers Gedebok Banana Results Delignification with Sodium Hydroxide Solvent (Naoh)

2018 ◽  
Vol 6 (02) ◽  
pp. 105-120
Author(s):  
Muhammad Rouf Suprayogi ◽  
Annisa Mufida ◽  
Edwin Azwar
Keyword(s):  
Tensile Strength ◽  
Compressive Strength ◽  
Lignin Content ◽  
Sound Intensity ◽  
Cellulose Fiber ◽  
Cellulose Content ◽  
Cellulose Powder ◽  
Drying Method ◽  
Normal Concrete ◽  
Sound Reduction

In composite science, desirable materials that are lighter but have the power and quality that can match or even exceed the material that has been there before. The purpose of this study was to investigate the effect of cellulose fiber addition from banana gedebok to tensile strength, compressive strength and damping of concrete composite sound. To achieve this objective, mixing of cellulose fibers with K-275 quality concrete mix with variation of 0% and 5% substitution in which the cellulose is varied in powder and wicker form. Delignification of lignin content from banana gedebok was done by soaking and drying method without any variation and yielding powder having cellulose content of 13,0388%, hemicellulose 18,2796% and lignin 0,6684%. This study produces concrete composites that have a tensile strength and a compressive strength lower than that of normal concrete. Normally reinforced concrete tensile strength value 94.5 kg / cm2, 71.4 kg / cm2 cellulose powder concrete and 90.3 kg / cm2 cellulose woven concrete. Normal concrete compressive strength value 334,22 kg / cm2, cellulose powder concrete 215,7 kg / cm2, and cellulose webbing concrete 157,98 kg / cm2. As for the power damping sound of cellulose webbing concrete has the highest damping power compared to other concrete with the absorbed sound intensity that is 52-68 dB

Ultrasound-assisted aqua-mediated synthesis of multi-substituted tetrahydropyridine-3-carboxylates using N-carboxymethyl-3-pyridinium hydrogensulfate ([N-CH2CO2H-3-pic]+HSO4−) as a new efficient ionic liquid catalyst

2021 ◽  
Vol 3 (6) ◽  
Author(s):  
Kobra Nikoofar ◽  
Fatemeh Shahriyari
Keyword(s):  
Ionic Liquid ◽  
Reaction Times ◽  
Aromatic Aldehydes ◽  
Environmentally Benign ◽  
H Nmr ◽  
Sonic Waves ◽  
Ft Ir ◽  
High Yields ◽  
Work Up ◽  
C Nmr

AbstractA simple, straightforward, and ultrasound-promoted method for the preparation of some highly functionalized tetrahydropyridines reported via pseudo five-component reaction of (hetero)aromatic aldehydes, different anilines, and alkyl acetoacetates in the presence of [N-CH2CO2H-3-pic]+HSO4−, as a novel ionic liquid, in green aqueous medium. The IL was synthesized utilizing simple and easily-handled substrates and characterized by FT-IR, 1H NMR, 13C NMR, GC-MASS, FESEM, EDX, and TGA/DTG techniques. The procedure contains some highlighted aspects which are: (a) performing the MCR in the presence of aqua and sonic waves, as two main important and environmentally benign indexes in green and economic chemistry, (b) high yields of products within short reaction times, (c) convenient work-up procedure, (d) preparing the new IL via simple substrates and procedure.

scholarly journals Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Preliminary hydrolysis and fermentation studies

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
WHINY HARDIYATI ERLIANA ◽  
Tri Widjaja ◽  
ALI ALTWAY ◽  
LILY PUDJIASTUTI
Keyword(s):  
Lactic Acid ◽  
Lignin Content ◽  
Agricultural Waste ◽  
Lactic Acid Production ◽  
Lactobacillus Rhamnosus ◽  
Lactobacillus Brevis ◽  
Reducing Sugar ◽  
Cellulose Content ◽  
Acid Pretreatment ◽  
Acid Fermentation

Abstract. Erliana WH, Widjaja T, Altway A, Pudjiastuti L. 2020. Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Hydrolysis and fermentation studies. Biodiversitas 21: 2281-2288. The increasing problems of global energy and the environment are the main reasons for developing products with new techniques through green methods. Sugar palm trunk waste (SPTW) has potential as agricultural waste because of its abundant availability, but it is not used optimally. This study was aimed to determine the effect of various microorganisms on increasing lactic acid production by controlling pH and temperature conditions in the fermentation process. SPTW contains 43.88% cellulose, 7.24% hemicellulose, and 33.24% lignin. The lignin content in SPTW can inhibit reducing sugar formation; the pretreatment process should remove this content. In the study, the pretreatment process was conducted using acid-organosolv. In the acid pretreatment, 0.2 M H2SO4 was added at 120oC for 40 minutes; organosolv pretreatment using 30% ethanol (v/v) at 107oC for 33 minutes was able to increase cellulose content by 56.33% and decrease lignin content by 27.09%. The pretreatment was followed by an enzymatic hydrolysis process with a combination of commercial cellulase enzymes from Aspergillus niger (AN) and Trichoderma reesei (TR), with variations of 0:1, 1:0, 1:1, 1:2 and 2:1. The best reducing sugar concentration was obtained with an AN: TR ratio of 1:2 to form reducing sugar from cellulose. Subsequently, lactic acid fermentation was carried out using lactic acid bacteria at 37oC and pH 6 incubated for 48 hours. The highest lactic acid concentration (33.292 g/L) was obtained using a mixed culture of Lactobacillus rhamnosus and Lactobacillus brevis to convert reducing sugar become lactic acid.

Negative gravitropism of Ginkgo biloba: growth stress and reaction wood formation

Holzforschung ◽  
2016 ◽  
Vol 70 (3) ◽  
pp. 267-274 ◽  
Author(s):  
Tatsuya Shirai ◽  
Hiroyuki Yamamoto ◽  
Miyuki Matsuo ◽  
Mikuri Inatsugu ◽  
Masato Yoshida ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Lignin Content ◽  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Wood Formation ◽  
Growth Stress ◽  
Reaction Wood ◽  
Cellulose Content ◽  
S2 Layer ◽  
Negative Gravitropism

Abstract Ginkgo (Ginkgo biloba L.) forms thick, lignified secondary xylem in the cylindrical stem as in Pinales (commonly called conifers), although it has more phylogenetic affinity to Cycadales than to conifers. Ginkgo forms compression wood-like (CW-like) reaction wood (RW) in its inclined stem as it is the case in conifers. However, the distribution of growth stress is not yet investigated in the RW of ginkgo, and thus this tissue resulting from negative gravitropism is still waiting for closer consideration. The present study intended to fill this gap. It has been demonstrated that, indeed, ginkgo forms RW tissue on the lower side of the inclined stem, where the compressive growth stress (CGS) was generated. In the RW, the micorofibril angle in the S2 layer, the air-dried density, and the lignin content increased, whereas the cellulose content decreased. These data are quite similar to those of conifer CWs. The multiple linear regression analysis revealed that the CGS is significantly correlated by the changes in the aforementioned parameters. It can be safely concluded that the negative gravitropism of ginkgo is very similar to that of conifers.

scholarly journals Comparative transcriptome analysis of Pteroceltis tatarinowii Maxim., an endemic fiber tree

2019 ◽  
Vol 48 (3) ◽  
pp. 547-557
Author(s):  
Hui-Jin Liu ◽  
Li Zhang ◽  
Yan-Nian Xu ◽  
Xiao-Ping Zhang ◽  
Xiao-Hong Li
Keyword(s):  
Protein Gene ◽  
Raw Materials ◽  
Lignin Content ◽  
Hierarchical Cluster ◽  
Raw Material ◽  
Limiting Factor ◽  
Cellulose Content ◽  
Three Samples ◽  
Phenylpropanoid Biosynthesis ◽  
Significant Expression

The bark of Pteroceltis tatarinowii Maxim., an endemic tree in Ulmaceae, is the main raw material for manufacturing Xuan Paper which is widely used in calligraphy and painting field. The characteristics of P. tatarinowii bark is the main limiting factor for the quality of Xuan Paper specially the content of cellulose and lignin. The molecular basis related to cellulose and lignin synthesis in P. tatarinowii would be helpful to understand and seek higher quality raw materials for Xuan Paper. RNA-seq was utilized to reveal transcriptome differences in P. tatarinowii from three far isolated localities (AL, JX and XA) under different climate environments. A total of 290 million reads were generated for further analysis in three libraries. In total, 2,850, 2,038 and 1,986 DEGs were identified in XA, JX and AL, respectively. Compared with the sample from XA, there were 822 up-regulated and 1706 down-regulated in AL sample. AL sample has 611 up-regulated genes and 647 down-regulated genes in comparison with JX sample. Comparing XA and JX samples, 443 were up-regulated and 1,783 were down-regulated in XA. Three samples had similar GO enrichment patterns. There were 19 and 9 genes identified as CESA and CSL (E-value less than 1.0E-20), respectively. Although no significant expression differences were found in three samples, KOB1, GPI-anchored protein gene and CTL1 were differently expressed, and KOB1 and GPI-anchored protein gene were up-regulated in JX. A number of the unigenes (474) that were involved in ‘phenylpropanoid biosynthesis’, were mostly not differently expressed. Only a few genes annotated as PAL, 4CL, C4H and CAD were significantly different in expression. In AL, 3 CAD and 1 PAL were up-regulated, whereas 6 CAD, 3 4CL and 1 HCT were up-regulated in XA, and 1 PAL, 2 4CL, 2 C4H in JX. JX sample had the highest cellulose content and XA sample had the highest lignin content, which being consistent with the hierarchical cluster analysis of differently expressed genes. Differences in the expression of these genes might influence the cellulose and lignin content.

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