scholarly journals Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Muhammad Arsalan Iqbal ◽  
Asghar Ali ◽  
Frieder Hadlich ◽  
Michael Oster ◽  
Henry Reyer ◽  
...  
Keyword(s):  
Egg Production ◽  
Target Genes ◽  
Laying Hens ◽  
Inositol Phosphate ◽  
Glutathione Metabolism ◽  
Mrna Targets ◽  
Inositol Phosphate Metabolism ◽  
Dietary Phosphorus ◽  
Different Levels ◽  
P Bioavailability

AbstractPhosphorus (P) and calcium (Ca) are critical for egg production in laying hens. Most of P in plant-based poultry diet is bound as phytic acid and needs to be hydrolysed before absorption. To increase P bioavailability, exogenous phytases or bioavailable rock phosphate is added in feed. There is growing evidence of the importance of miRNAs as the epicentre of intestinal homeostasis and functional properties. Therefore, we demonstrated the expression of miRNA profiles and the corresponding target genes due to the different levels of P (recommended vs. 20% reduction) and/or Ca (recommended vs. 15% reduction) in feed. Jejunal miRNA profiles of Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) laying hens strains were used (n = 80). A total of 34 and 76 miRNAs were differentially expressed (DE) in the different diet groups within LSL and LB strains respectively. In LSL, the DE miRNAs and their targets were involved in calcium signaling pathway, inositol phosphate metabolism, and mitochondrial dysfunction. Similarly, in LB miRNAs targets were enriched in metabolic pathways such as glutathione metabolism, phosphonate metabolism and vitamin B6 metabolism. Our results suggest that both strains employ different intrinsic strategies to cope with modulated P and Ca supply and maintain mineral homeostasis.

scholarly journals The effect of raw soybeans in mixtures for laying hens on production performance and the relative weight of the pancreas

2014 ◽  
Vol 30 (1) ◽  
pp. 115-123
Author(s):  
V. Petricevic ◽  
M. Lukic ◽  
Z. Pavlovski ◽  
Z. Skrbic ◽  
Z. Jokic ◽  
...  
Keyword(s):  
Food Consumption ◽  
Egg Production ◽  
Laying Hens ◽  
Relative Weight ◽  
Production Performance ◽  
Heat Treated ◽  
Body Weights ◽  
Standard Level ◽  
Production Body ◽  
Different Levels

The study was conducted on Isa Brown hybrid hens at the age of 49-57 weeks. The effect of using different levels of share of raw soybean of two varieties in mixtures for feeding hens on egg production, body weight, food consumption, the occurrence of defective eggs, mortality and the relative weight of the pancreas was studied. The possibility of replacing the heat-treated soybean grains, varieties Lana, with reduced trypsin inhibitor (TI) and Lydia with a standard level of TI, with raw soybean grains was examined. The research was conducted on the principle of two factorial experiment 2 x 4 (2 varieties x 4 levels of share of raw grain in the mixture) with a total of 8 diet treatments and 4 replicates per each treatment. In the first 5 weeks of the study, the differences in the number of eggs produced under the influence of tested factors were not significant. Under the influence of soybean varieties, the level of share of raw soybean and interaction of the studied factors showed significant differences (p<0.01) after 53 week of age. The use of soy with lower TI in the diet for laying hens resulted in a significantly greater capacity compared to standard variety. The share of raw soybean grains of 8 % in the mixtures significantly reduced the number of eggs laid. The differences in body weights, food consumption, occurrence of defective eggs and the relative weight of the pancreas were not significantly influenced by the studied factors or by their interaction effect.

scholarly journals The Effects of Different Levels Chlorell Microalgae on Performance and Immune Response of Laying Hens Under Heat Stress Condition

2015 ◽  
Vol 9 (2) ◽  
pp. 71-74 ◽  
Author(s):  
Nasroallah Moradi Kor ◽  
Neda Mohamadi
Keyword(s):  
Immune Response ◽  
Heat Stress ◽  
Egg Production ◽  
Stress Condition ◽  
Linear Models ◽  
Laying Hens ◽  
Egg Mass ◽  
Randomized Design ◽  
Heat Stress Condition ◽  
Different Levels

This study was conducted to determine the effects of different levels microalgae Chlorell sp. on performance of laying hens under heat stress condition. A completely randomized design experiment, with 7 replicates and 6 treatments (9 birds per replicate) was carried out by allotting 378 white Lohman strain laying hens at 65 weeks of age. Six experimental treatments were continual addition of 0, 100, 200, 300,400 ppm microalgae Chlorell and 400 ppm toein to drinking warer were fed to laying hens for 9 weeks. Egg production, egg weight, egg mass output and feed intake were determined. Antibody production against sheep red blood cells (SRBC) also was measured. Data  were  analyzed  by  ANOVA  using  General  Linear Models procedure of  SAS software and comparison of means  were  compared  using  Duncan’s  multiple  range test. The results of this experiment showed that microalgae Chlorell had no effect on productive performance. Adding microalgae Chlorell to the drinking water improved immune response of laying hens and a more positive effect was observed when 400 ppm added to the water. It is concluded that the addition of microalgae Chlorell to the water drinking of laying hens improved immune response of laying hens.DOI: http://dx.doi.org/10.3126/ijls.v9i2.12058 International Journal of Life Sciences 9 (2) : 2015; 71-74

scholarly journals Flour of mushroom Agaricus blazei as additive functional in food of laying hens

2019 ◽  
Vol 20 ◽  
Author(s):  
Iolanda Silveira Freitas ◽  
Michele de Oliveira Mendonça ◽  
Izabela Silveira Freitas ◽  
Onofre Barroca de Almeida Neto ◽  
David Garcia Ferreira ◽  
...  
Keyword(s):  
Immune System ◽  
Feed Intake ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Experimental Unit ◽  
Egg Mass ◽  
Agaricus Blazei ◽  
Feed Conversion ◽  
Different Levels

SUMMARY The current study aims to evaluate the zootechnical performance, quality of eggs, and the immune system of the laying hen's upon supplementing their feed with the flour of the mushroom Agaricus blazei. A total of 210 Hisex Brown® hens, 67 weeks old, were distributed in a completely randomized design with five treatments (control [0%], 0.04%, 0.08%, 0.12% and 0.16% of mushroom meal) with seven replicates and six birds per experimental unit. Were evaluated the zootechnical performance (feed intake, egg production [per day and housed], marketable egg production, egg weight and egg mass, feed conversion per dozen and egg mass, and viability);egg quality (specific weight, Haugh units, weight and percentage of components, shell thickness and yellow intensity of the egg yolks); and the hemogram (erythrogram and leukogram). Mainly, different levels of mushroom meal, were includedin laying hens feeds significantly (p<0.05)egg mass and feed intake. However, the leukogram and the erythrogram remained within the reference values, indicating that the birds were healthy. The addition of different levels of Agaricus blazei flour in the laying hens feed did not promote changes in the egg quality, in the immune system, and in the zootechnical performance, except for the feed intake which reduced with the inclusion of mushroom flour and eggs mass in which the 0.07% level promoted the maximization of this parameter.

scholarly journals Immunomodulatory Effects of Dietary Phosphorus and Calcium in Two Strains of Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 129
Author(s):  
Tanja Hofmann ◽  
Sonja Schmucker ◽  
Vera Sommerfeld ◽  
Korinna Huber ◽  
Markus Rodehutscord ◽  
...  
Keyword(s):  
T Cells ◽  
Immune System ◽  
Egg Production ◽  
Laying Hens ◽  
Animal Health ◽  
Negative Effects ◽  
Mineral Phosphorus ◽  
Immune Parameters ◽  
Dietary Phosphorus ◽  
The Impact

Insufficient nutrient supply can impair the immune system, which is important for animal health and welfare. Since chicken can partly hydrolyze phytate, which is the primary phosphorus storage in plant seeds, a reduction of mineral phosphorus in the diets could be an option for more sustainable egg production. Laying hens require high concentrations of calcium that might inhibit the function of endogenous enzymes for phytate hydrolyzation. The objective of this study was to characterize the impact of standard and reduced dietary phosphorus and calcium concentrations on the number and functionality of immune cells in the peripheral and gut-associated immune system in a white and brown laying hen strain. Reduced mineral phosphorus enhanced several immune parameters such as B cells in blood and IgA concentrations in bile in both strains, and peripheral monocytes and T cells in cecal tonsils in brown hens. Reduced calcium levels resulted in lower numbers of T cells in blood and cecal tonsils in both strains, suggesting negative effects on adaptive immunity. Differences between the two strains were found in almost all immune parameters. Results suggest a potentially beneficial effect of reduced dietary mineral phosphorus on the immune system that is dependent on the genetic background.

scholarly journals The Excretion of Ca, Mg, Zn and Cu Through Excreta of Laying Hens Fed Two Different Levels of Protein with and without Phytase

2019 ◽  
Vol 7 (1) ◽  
pp. 68
Author(s):  
Skender Muji ◽  
Alltane Kryeziu ◽  
Muhamet Kamberi ◽  
Ragip Kastrati ◽  
Nuridin Mestani
Keyword(s):  
Crude Protein ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Mass ◽  
Egg Weight ◽  
Forced Draft Oven ◽  
Forced Draft ◽  
Dietary Treatments ◽  
Mg Content ◽  
Different Levels

An 8-week experiment was conducted to study the effect of added Natuphos® 5000 phytase in corn–soybean meal-based diets on laying hens fed different levels of crude protein (CP) (14 and 17%). Two levels of phytase enzyme were used: 0 and 600 Phytase Units (FTU)/kg feed. The experiment used 144 Hisex Brown laying hens in a 2 × 2 factorial design. Four treatments and three replicates per treatment with 12 hens per replicate were used. Egg production was recorded daily while egg weight was assessed on 13th and 14th day of each two-week period to calculate total egg mass. Total excreta were collected and approximately 10% of the amount was used for analysis after drying in a forced draft oven. The results showed no significant effect of added phytase on excreta Calcium (Ca) content, but significantly lower Magnesium (Mg) content was observed with phytase (3.54, 2.48, 3.13 and 2.75 % for hens fed 14% CP no phytase, 14% CP + phytase, 17% CP no phytase, and 17% CP + phytase, respectively). Added phytase also significantly decreased Mg excretion measured as grams/kg of egg mass (21.43, 12.47, 16.76 and 14.75 g/kg egg mass for hens of respective dietary treatments. Phytase had a strong effect on Zink (Zn) levels with 438.96, 369.17, 434.38 and 374.58 mg Zn/kg dry excreta of hens. Similar results were observed with Cu. Added phytase significantly reduced the excreta content and the excretion of Cu. The results of this experiment indicate that adding 600 FTU to laying hen diets containing 14% CP decreases the excretion of Mg, Zn and Cu without any adverse effects on the egg mass produced of laying hens.

scholarly journals Effects of Different Levels of Dietary Crude Protein on Egg Production and Quality in Laying Hens during Early Stage of Egg Production

2013 ◽  
Vol 40 (4) ◽  
pp. 361-368
Author(s):  
Yun Kyung Choo ◽  
Hyung Joo Kwon ◽  
Sung Taek Oh ◽  
Yong Ran Kim ◽  
Eun Jib Kim ◽  
...  
Keyword(s):  
Crude Protein ◽  
Egg Production ◽  
Laying Hens ◽  
Early Stage ◽  
Dietary Crude Protein ◽  
Different Levels

scholarly journals Effect of suboptimal levels of non-phytate phosphorus and exogenous phytase on precaecal digestibility of phosphorus and calcium in laying hens

2017 ◽  
Vol 62 (No. 11) ◽  
pp. 473-481
Author(s):  
A. Musilova ◽  
M. Lichovnikova ◽  
L. Kupcikova ◽  
V. Anderle
Keyword(s):  
Egg Production ◽  
Phosphorus Content ◽  
Laying Hens ◽  
Phosphorus Concentration ◽  
Posterior Half ◽  
Daily Calcium Intake ◽  
Ileal Digestibility ◽  
Phytate Phosphorus ◽  
Dietary Phosphorus ◽  
Significant Difference

The precaecal digestibility of phosphorus and calcium in laying hens was evaluated at two dietary levels of non-phytate phosphorus (NPP; 1.58 and 2.03 g/kg) and two levels of exogenous 3-phytase (F; 0 and 300 phytase units (FTU)/kg). A total of 192 ISA Brown hens were used for the study, and were housed in enriched cages (8 per cage). At the end of the experiment, which was the peak of the egg production, the content of the posterior half of the ileum from each hen was collected. The lower level of NPP significantly (P &lt; 0.05) improved the precaecal phosphorus digestibility coefficient. There was no significant difference in the amount of absorbed phosphorus between the lower and the higher level of NPP. The level of NPP with supplementation of exo-genous phytase had a significant effect (P &lt; 0.001) on the concentration of phosphorus in ileum, while the higher level of NPP increased phosphorus concentration in the ileum and phytase decreased phosphorus content in the ileal digesta. The phytase had no significant effect on phosphorus precaecal digestibility. There was no significant effect of the both factors, level of NPP, and exogenous phytase on the daily calcium intake, the amount of absorbed calcium, the content of calcium in ileal digesta, and on the precaecal digestibility of calcium. However a significant effect (P &lt; 0.05) of the interaction between NPP and phytase was observed on the amount of absorbed calcium and on the precaecal digestibility coefficient. It can be concluded that the estimation of phosphorus ileal digestibility depends on the level of dietary phosphorus.

scholarly journals Effects of Platycodins Folium on Depression in Mice Based on a UPLC-Q/TOF-MS Serum Assay and Hippocampus Metabolomics

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1712 ◽  
Author(s):  
Cuizhu Wang ◽  
Hongqiang Lin ◽  
Na Yang ◽  
Han Wang ◽  
Yan Zhao ◽  
...  
Keyword(s):  
Acid Metabolism ◽  
Inositol Phosphate ◽  
Preference Test ◽  
Tail Suspension Test ◽  
Arachidonic Acid Metabolism ◽  
Antidepressant Effect ◽  
Glutathione Metabolism ◽  
Triterpenoid Saponins ◽  
Inositol Phosphate Metabolism ◽  
Tof Ms

Major depressive disorder (MDD), also known as depression, is a state characterized by low mood and aversion to activity. Platycodins Folium (PF) is the dried leaf of Platycodon grandiflorum, with anti-inflammatory and antioxidative activities. Our previous research suggested that PF was rich in flavonoids, phenols, organic acids, triterpenoid saponins, coumarins and terpenoids. This study aimed to investigate the antidepressant effect of PF using lipopolysaccharide (LPS)-induced depressive mice. Several behavior tests (sucrose preference test (SPT), forced swimming test (FST) and tail suspension test (TST)) and biochemical parameters (IL-6, TNF-α and SOD levels) were used to evaluate the antidepressive effect of PF on LPS-induced depression model. Furthermore, a UPLC-Q/TOF-MS-based metabolomics approach was applied to explore the latent mechanism of PF in attenuating depression. As a result, a total of 21 and 11 metabolites that potentially contribute to MDD progress and PF treatment were identified in serum and hippocampus, respectively. The analysis of metabolic pathways revealed that lipid metabolism, amino acid metabolism, energy metabolism, arachidonic acid metabolism, glutathione metabolism and inositol phosphate metabolism were disturbed in a model of mice undergoing MDD and PF treatment. These results help us to understand the pathogenesis of depression in depth, and to discover targets for clinical diagnosis and treatment. They also provide the possibility of developing PF into an anti-depressantive agent.

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