scholarly journals Effect of surface energy and roughness on cell adhesion and growth – facile surface modification for enhanced cell culture

RSC Advances ◽  
2021 ◽  
Vol 11 (25) ◽  
pp. 15467-15476
Author(s):  
B. Majhy ◽  
P. Priyadarshini ◽  
A. K. Sen
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Surface Modification ◽  
Cell Adhesion ◽  
Surface Energy ◽  
Polymeric Surfaces ◽  
Cellular Processing ◽  
Effect Of Surface

In vitro, cellular processing on polymeric surfaces is fundamental to the development of biosensors, scaffolds for tissue engineering and transplantation.

scholarly journals Surface modification of small intestine submucosa in tissue engineering

2020 ◽  
Vol 7 (4) ◽  
pp. 339-348 ◽  
Author(s):  
Pan Zhao ◽  
Xiang Li ◽  
Qin Fang ◽  
Fanglin Wang ◽  
Qiang Ao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Small Intestine ◽  
Cell Adhesion ◽  
Great Influence ◽  
Small Intestine Submucosa ◽  
Proliferation And Differentiation ◽  
Tissue Reconstruction

Abstract With the development of tissue engineering, the required biomaterials need to have the ability to promote cell adhesion and proliferation in vitro and in vivo. Especially, surface modification of the scaffold material has a great influence on biocompatibility and functionality of materials. The small intestine submucosa (SIS) is an extracellular matrix isolated from the submucosal layer of porcine jejunum, which has good tissue mechanical properties and regenerative activity, and is suitable for cell adhesion, proliferation and differentiation. In recent years, SIS is widely used in different areas of tissue reconstruction, such as blood vessels, bone, cartilage, bladder and ureter, etc. This paper discusses the main methods for surface modification of SIS to improve and optimize the performance of SIS bioscaffolds, including functional group bonding, protein adsorption, mineral coating, topography and formatting modification and drug combination. In addition, the reasonable combination of these methods also offers great improvement on SIS surface modification. This article makes a shallow review of the surface modification of SIS and its application in tissue engineering.

Poly(glycerol sebacate) – a revolutionary biopolymer

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Israd H. Jaafar ◽  
Sabrina S. Jedlicka ◽  
John P. Coulter
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Culture ◽  
Surface Modification ◽  
Mammalian Cell Culture ◽  
Chemical Properties ◽  
Tunable Mechanical Properties ◽  
And Chemical Properties

Abstract Novel materials possessing physical, mechanical, and chemical properties similar to those found in vivo provide a potential platform for building artificial microenvironments for tissue engineering applications. Poly(glycerol sebacate) is one such material. It has tunable mechanical properties within the range of common tissue, and favorable cell response without surface modification with adhesive ligands, and biodegradability. In this chapter, an overview of the material is presented, focusing on synthesis, characterization, microfabrication, use as a substrate in in vitro mammalian cell culture, and degradation characteristics.

Fabrication and characterization of customized tubular scaffolds for tracheal tissue engineering by using solvent based 3D printing on predefined template

2021 ◽  
Vol 27 (2) ◽  
pp. 421-428
Author(s):  
Rudranarayan Kandi ◽  
Pulak Mohan Pandey ◽  
Misba Majood ◽  
Sujata Mohanty
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
3D Printing ◽  
Chemical Properties ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
In Vitro Cytotoxicity ◽  
Content Type ◽  
Tracheal Tissue

Purpose This paper aims to discuss the successful fabrication of customized tubular scaffolds for tracheal tissue engineering with a novel route using solvent-based extrusion 3D printing. Design/methodology/approach The manufacturing approach involved extrusion of polymeric ink over a rotating predefined pattern to construct customized tubular structure of polycaprolactone (PCL) and polyurethane (PU). Dimensional deviation in thickness of scaffolds were calculated for various layer thicknesses of 3D printing. Physical and chemical properties of scaffolds were investigated by scanning electron microscope (SEM), contact angle measurement, Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD). Mechanical characterizations were performed, and the results were compared to the reported properties of human native trachea from previous reports. Additionally, in vitro cytotoxicity of the fabricated scaffolds was studied in terms of cell proliferation, cell adhesion and hemagglutination assay. Findings The developed fabrication route was flexible and accurate by printing customized tubular scaffolds of various scales. Physiochemical results showed good miscibility of PCL/PU blend, and decrease in crystalline nature of blend with the addition of PU. Preliminary mechanical assessments illustrated comparable mechanical properties with the native human trachea. Longitudinal compression test reported outstanding strength and flexibility to maintain an unobstructed lumen, necessary for the patency. Furthermore, the scaffolds were found to be biocompatible to promote cell adhesion and proliferation from the in vitro cytotoxicity results. Practical implications The attempt can potentially meet the demand for flexible tubular scaffolds that ease the concerns such as availability of suitable organ donors. Originality/value 3D printing over accurate predefined templates to fabricate customized grafts gives novelty to the present method. Various customized scaffolds were compared with conventional cylindrical scaffold in terms of flexibility.

scholarly journals Surface Characteristics and Cell Adhesion Behaviors of the Anodized Biomedical Stainless Steel

2020 ◽  
Vol 10 (18) ◽  
pp. 6275
Author(s):  
Heng-Jui Hsu ◽  
Chia-Yu Wu ◽  
Bai-Hung Huang ◽  
Chi-Hsun Tsai ◽  
Takashi Saito ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Stainless Steel ◽  
Cell Adhesion ◽  
Oxide Layer ◽  
Photoelectron Spectroscopy ◽  
X Ray ◽  
In Vitro Cell Culture ◽  
Cell Culture Assay

In this study, an electrochemical anodizing method was applied as surface modification of the 316L biomedical stainless steel (BSS). The surface properties, microstructural characteristics, and biocompatibility responses of the anodized 316L BSS specimens were elucidated through scanning electron microscopy, X-ray photoelectron spectroscopy, X-ray diffractometry, transmission electron microscopy, and in vitro cell culture assay. Analytical results revealed that the oxide layer of dichromium trioxide (Cr2O3) was formed on the modified 316L BSS specimens after the different anodization modifications. Moreover, a dual porous (micro/nanoporous) topography can also be discovered on the surface of the modified 316L BSS specimens. The microstructure of the anodized oxide layer was composed of amorphous austenite phase and nano-Cr2O3. Furthermore, in vitro cell culture assay also demonstrated that the osteoblast-like cells (MG-63) on the anodized 316L BSS specimens were completely adhered and covered as compared with the unmodified 316L BSS specimen. As a result, the anodized 316L BSS with a dual porous (micro/nanoporous) oxide layer has great potential to induce cell adhesion and promote bone formation.

scholarly journals Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

2008 ◽  
Vol 19 (7) ◽  
pp. 837-852 ◽  
Author(s):  
R. Tzoneva ◽  
B. Seifert ◽  
W. Albrecht ◽  
K. Richau ◽  
A. Lendlein ◽  
...  
Keyword(s):  
Surface Modification ◽  
Cell Adhesion ◽  
Endothelial Cell ◽  
And Function ◽  
Endothelial Cell Adhesion ◽  
Effect Of Surface

scholarly journals An Assessment of Cell Culture Plate Surface Chemistry for in Vitro Studies of Tissue Engineering Scaffolds

2015 ◽  
Vol 6 (4) ◽  
pp. 1054-1063 ◽  
Author(s):  
Alexander Röder ◽  
Elena García-Gareta ◽  
Christina Theodoropoulos ◽  
Nikola Ristovski ◽  
Keith Blackwood ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Surface Chemistry ◽  
In Vitro Studies ◽  
Plate Surface ◽  
Tissue Engineering Scaffolds ◽  
Culture Plate

Preliminary testing of silicone-urethane elastomers as substrates in human cell culture

e-Polymers ◽  
2007 ◽  
Vol 7 (1) ◽  
Author(s):  
Malgorzata Lewandowska-Szumieł ◽  
Janusz Kozakiewicz ◽  
Piotr Mrówka ◽  
Agnieszka Jurkowska ◽  
Edyta Sienkiewicz-Łatka ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Blood Platelets ◽  
Human Fibroblasts ◽  
Medical Implants ◽  
Tissue Engineering Scaffolds ◽  
Xtt Assay ◽  
In Vitro Biocompatibility ◽  
Different Types

AbstractSilicone-urethanes, polymers combining the characteristics of two widely used biomaterials, i.e. polyurethanes and silicones, are highly valued in many applications, including medical implants. To assess properties of these materials in contact with living cells, a set of different silicone-urethane materials, candidates for tissue engineering scaffolds, was synthesized and characterized. Two different oligomeric siloxane diols: Tegomer-2111 (Teg) and KF-6001 (KF), and two different types of diisocyanate, MDI and IPDI, were used in synthesis. Blood platelets adhesion to surfaces of selected materials showed a higher thrombogenicity of material based on Teg. Human fibroblasts were used in in vitro biocompatibility tests. The viability of cells cultured on silicone-urethanes was tested by XTT assay. Teg-based silicone-urethanes showed a significantly higher biocompatibility than those based on KF. Materials based on MDI compared to IPDI were found to be significantly more favoured by cells, not necessarily due to the type of diisocyanate but maybe also because of the necessity of using potentially toxic catalyst which accompanies the use of IPDI. Our studies indicate that silicone-urethanes are potent materials for tissue engineering products development. On the basis of the observations performed in cell culture, Tegomer- 2111 as oligomeric siloxane diol and MDI as diisocyanate are recommended as starting materials for silicone-urethane scaffolds synthesis.

Mechanically Enhanced Precipitation of Phase-Inversion Sprayed Polyurethane Scaffold May Be Used to Match Tissue Specific Anisotropy

2009 ◽  
Author(s):  
James P. Kennedy ◽  
Robert W. Hitchcock
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Culture ◽  
Pore Size ◽  
Phase Inversion ◽  
Anisotropy Ratio ◽  
Material Anisotropy ◽  
Polyurethane Scaffold ◽  
Stiffness Anisotropy

Methods of creating a scaffold for tissue engineering that allow for modification of properties such as pore size, porosity, and anisotropy are essential for tissue engineering applications. For example the pore size and material anisotropy have been shown to affect cardiomyocyte elongation and alignment [1]. Phase-inversion spray polymerization (PISP) is a method for rapidly precipitating polymers onto a surface by depositing the polymer solution simultaneously with a nonsolvent, and may be used to create biocompatible scaffolds of engineered morphological and mechanical properties by varying the solubility of the polymer in the nonsolvent [2]. We report here on the fabrication of scaffolds using different nonsolvents and methods of in-process elongation that allow for control of stiffness, anisotropy ratio, porosity, and in vitro cell culture.

Effect of surface modification on the in vitro calcium phosphate growth on the surface of poly(methyl methacrylate) and bioactivity

2010 ◽  
Vol 76 (1) ◽  
pp. 326-333 ◽  
Author(s):  
Sun-Mi Choi ◽  
Won-Kyu Yang ◽  
Yong-Won Yoo ◽  
Woo-Kul Lee
Keyword(s):  
Surface Modification ◽  
Calcium Phosphate ◽  
Methyl Methacrylate ◽  
Poly Methyl Methacrylate ◽  
Effect Of Surface

Improvement of Bioactivity of Collagen/Alginate Scaffolds by Hydroxyapatite for Tissue Engineering Cartilage

2008 ◽  
Vol 396-398 ◽  
pp. 445-448 ◽  
Author(s):  
J. Sun ◽  
R. Wang ◽  
L. Zheng ◽  
Yan Fei Tan ◽  
Yu Mei Xiao ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Tissue Engineering ◽  
Cell Culture ◽  
Composite Film ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
The Poor ◽  
Pure Collagen ◽  
Good Biocompatibility

With good biocompatibility, collagen is often used in cartilage tissue engineering. Collagen/alginate composite was hoped to improve the poor mechanical property of pure collagen but the biocompatibity was decreased. In this study, hydroxyapatite (HA) particles were used to get collagen/alginate/HA (CAHA) composite film to enhance the bioactivity properties. The bioactivity of the composite was investigated by in vitro co-culture with chondrocytes. During the 6-day cell culture in vitro, the composite showed a significant improvement in promoting proliferation and maintaining morphology/phenotype of the chondrocytes over collagen/alginate composite by MTT, SEM, fluorescent and immunohistochemical assays. Cytocompatibility and cytoviablility of CAHA even come up to that of collagen film alone. The results indicated that the composite film may provide an appropriate environment for the proliferation and maintaining the morphology and phenotype of chondrocytes and have a potential clinical application in the cartilage tissue engineering field.

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