scholarly journals The Effectiveness of Tamarindus indica Extract in Total Osteoblast Cell of Male Wistar Rat’s Femur Bone Induced by Aluminium

2020 ◽  
Vol 6 (2) ◽  
pp. 110
Author(s):  
Indah Pratiwi ◽  
Rena Normasari ◽  
Rony Prasetyo
Keyword(s):  
Data Analysis ◽  
Wistar Rats ◽  
Control Group ◽  
Tamarindus Indica ◽  
Control Group Design ◽  
Chain Reactions ◽  
Femur Bone ◽  
Post Test ◽  
Male Wistar Rats

Aluminum is widely used in life but includes non-essential metals toxic to the human body that most accumulates in the femur. Aluminum is toxic because it increases the number of free radicals resulting in oxidative stress that causes osteoblast apoptosis. Tamarindus indica has the most potent antioxidant effect on the seed. The polyphenol content in Tamarindus indica seed are myricetin, procyanidin B2, and caffeic acid. Antioxidants in Tamarindus indica seed have mechanism of donating electrons from the -OH group in the phenolic ring to stop oxidative chain reactions and prevent the formation of hydroxyl radicals and lipid peroxidation that play a role in cell apoptosis. The purpose of this study  to determine the effect of giving Tamarindus indica extract on the total osteoblasts of femur bone in male wistar rats induced by aluminum. This research is a true experimental in vivo with randomized post-test only control group design using 25 male Wistar rats divided into five groups. Aluminum is given orally dose 300mg/kgBB and Tamarindus indica extract dose 25, 50, 100mg/kgBB for 10 weeks. The results of this study indicate the average total of osteoblasts in groups K, P1, P2, P3, P4 are 15.21 ± 1.71; 18.48 ± 3.65; 17.8 ± 7.05; 17.13 ± 1.16; 16.74 ± 5.71. Data analysis used the Kruskal Wallis test. The results of data analysis showed that there was no significant differences in all groups (p = 0.581). Based on the results of this study, Tamarindus indica extract that was given to male wistar rats induced by aluminum for 10 weeks had no effect on the total osteoblasts.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

scholarly journals ROLE OF COMBINATION CASEIN AND LACTOFERRIN BOVINE’S COLLOSTRUM AS A PULP CAPPING ON MACROPHAGE EXPRESSION IN MALE WISTAR RATS

2021 ◽  
Vol 8 (2) ◽  
pp. 156
Author(s):  
Endah Ariyati Eko Ningtyas ◽  
Oedijani Santoso ◽  
Udadi Sadhana ◽  
Siti Sunarintyas
Keyword(s):  
Therapeutic Effect ◽  
Wistar Rats ◽  
Therapeutic Potential ◽  
Healing Process ◽  
Bovine Colostrum ◽  
Control Group ◽  
Control Group Design ◽  
Reparative Dentin ◽  
Post Test ◽  
Male Wistar Rats

ABSTRACTBackground: Inflammatory and/or non-inflammatory processes play a role in stimulating pulp repair and the formation of hard tissue, namely reparative dentin. Macrophages play a role in the pathogenesis and chronic inflammatory disorders. The combination casein lactoferrin of bovine colostrum as an immunomodulator has therapeutic potential. This study aims to determine the therapeutic effect and duration of application of the combination of casein lactoferrin of bovine colostrum, on the expression of macrophages as pulp capping.Method: This study was a true experimental laboratories post test only control group design, consisting of three groups of 60 male wistar rats with 4 observation times, namely day to day 7, 14, 21 and 28 each of 5 mice. The maxillary 1st molars were prepared until the roof of the pulp was exposed. Three groups, namely the combination of casein and lactoferrin bovine colostrum (CKL) and calcium hydroxide (K1) and the untreated group (K0). Each group was filled with glassionomer as a permanent restoration. The tissue was made histological preparations with hematoxylin-eosin staining and the number of macrophages were counted, then analyzed by two way ANOVA and post hoc LSD tests.Result: The results showed that the therapeutic effect and duration of application of the combination of casein and lactoferrin bovine colostrum on the expression of macrophages as pulp cappingConclusion: The combination of casein and lactoferrin of bovine colostrum as capping material can increase the number of macrophages in the healing process of dental pulp.

scholarly journals PENGARUH PEMBERIAN SAGU DIBANDING NASI TERHADAP BERAT BADAN TIKUS WISTAR

e-CliniC ◽  
2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Eny A. Watumlawar ◽  
Sarah M. Warouw ◽  
Stefanus Gunawan
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Wilcoxon Test ◽  
Control Group ◽  
Increase Body Weight ◽  
Control Group Design ◽  
Tissue Mass ◽  
Group Design ◽  
Post Test ◽  
Male Wistar Rats

Abstract: Weight is one measure that gives description of the tissue mass including body fluids. Weight change is nfluenced by nutriment consumption. Sago contains high carbohydrates that can increase body weight. This study aimed to obtain the effect of sago compared to rice to body weight of wistar rats. This was a random laboratory experimental pre-post test with control group design. Subjects were male wistar rats, aged 5-6 months. The rats were divided in two groups: rice group as control and sago group. Sago was cooked in papeda form as much as 75 g of dried sago and 300 mL water. The rats were fed for 2 weeks. Data were analyzed by using the Wilcoxon test. The results showed that the sago group showed an increase of body weight significantly (p=0,001) meanwhile the rice group lose body weight significantly (p=0,001). Conclusion: Sago can increase body weight of wistar rats significantly.Keywords: body weight, sago, riceAbstrak: Berat badan merupakan salah satu ukuran yang memberikan gambaran massa jaringan termasuk cairan tubuh. Salah satu yang memengaruhi berat badan yaitu dengan mengonsumsi makanan bergizi. Sagu memiliki kandungan karbohidrat (pati) yang besar dan dapat meningkatkan berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sagu terhadap berat badan dibanding nasi pada tikus wistar. Desain penelitian ini ialah eksperimental laboratorium acak pre-post test with control group design. Subjek penelitian ialah tikus wistar jantan berusia 5-6 bulan dibagi atas 2 kelompok: kelompok nasi (kontrol) dan kelompok sagu. Sagu dimasak dalam bentuk papeda sebanyak 75 g sagu kering dan 300 mL air. Tikus diperlihara selama 2 minggu. Berat badan diukur dengan menggunakan timbangan. Data dianalisis dengan uji Wilcoxon. Hasil penelitian menunjukkan bahwa pemberian sagu sebanyak 2 cc setiap hari selama seminggu mampu meningkatkan berat badan secara bermakna (p = 0,001) sedangkan pemberian nasi menurunkan berat badan (p=0,001). Simpulan: Pemberian sagu dapat meningkatkan berat badan tikus wistar secara bermakna.Kata kunci: berat badan, sagu, nasi

scholarly journals THE EFFECT OF SEMBUNG LEAF EXTRACT (BLUMEA BALSAMIFERA) ON THE NUMBER AND DIAMETER OF RATS LEYDIG CELLS INDUCED BY HIGH-FAT DIET

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Gede Widhiantara ◽  
Anak Agung Ayu Putri Permatasari ◽  
Putu Angga Wiradana
Keyword(s):  
Treatment Group ◽  
Leydig Cells ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Control Group Design ◽  
Core Diameter ◽  
Post Test ◽  
Male Wistar Rats ◽  
The Mean

This study aims to determine the morphology of Leydig cells in hyperlipidemic wistar rats after having administered with sembung (Blumea balsamifera) extract orally. This study utilised a randomized post-test only control group design. The sample in this study were 16 adult male wistar rats (Rattus novergicus) aged 3-4 months with a body weight of 150-200 grams, which were equally and randomly divided into two groups, namely control group (hyperlipidaemia and sterile aquadest) and treatment group (hyperlipidaemia and sembung leaf extract). Hyperlipidaemia was induced with lard administration for 50 days. Data were analysed statistically using the Independent t-test. The results showed that the number of Leydig cells in the treatment group was higher than the control group with 68.13 ± 1.89 and 55.63 ± 1.92 cells respectively (P <0.05). In addition, the mean Leydig cell core diameter of the control group, 5.00 ± 0.34 µm, was smaller compared to the treatment group which was 5.80 ± 0.20 µm (P <0.05). It can be inferred that sembung leaf extract provides a protective effect against damage to Leydig cells due to hyperlipidaemia.

scholarly journals The effect of black cumin oil (Nigella sativa) on the reproduction of male Wistar rats

2021 ◽  
Vol 1 (2) ◽  
pp. 45-49
Author(s):  
Syahran Wael ◽  
Didik Wahyudi ◽  
Tayeb Wael ◽  
Zaid Mohamed Jaber
Keyword(s):  
Wistar Rats ◽  
Nigella Sativa ◽  
Cellular Damage ◽  
Control Group ◽  
Control Group Design ◽  
Black Cumin ◽  
Group Design ◽  
Nigella Sativa Oil ◽  
Post Test ◽  
Male Wistar Rats

Nigella sativa oil is antioxidant compound has the effect that serves to prevent cellular damage. The effect of Nigella sativa oil in the motility and total count of spermatozoa wistar rats. Experimental research with the design of post test only control group design. Wistar rats consists of 24 head and divided into 4 groups consist of control and treatment group. The control group was distilled. The first treatment was of Nigella sativa oil everydays as much with dose 150 mg/kgbw, 250 mg/kgbw, and 350 mg/kgbw, for 16 days, . Statistic test for motility and count of sperm wistar rats use the Kruskal-Wallis followed by Mann Whitney test. Avarage value of motility in the control (21.67±9.832) its lower than treatment. In dose 350 mg/kgbw its highest (52.33±13.292) compare in the treatment 250 mg/kgbw (40.67±17.512) and 150 mg/kgbw (30.67±8.165). avarage value of count sperm in the control (130.83±41.877) its lower than treatments. In dose 350 mg/kgbw its highest (199.67±23.480) compare in the treatment 250 mg/kgbw (187.50±74.538) and 150 mg/kgbw (140.83±32.568). Administration of Nigella sativa oil occur to enhancement motility and number of spermatozoa wistar rats.

scholarly journals Pengaruh ekstrak melon terhadap kadar HbCO pada tikus Wistar jantan yang dipapar asap rokok

2017 ◽  
Vol 13 (3) ◽  
pp. 105
Author(s):  
Yuyun Erlina Susanti ◽  
Bambang Wirjatmadi ◽  
Merryana Adriani
Keyword(s):  
Cigarette Smoke ◽  
Wistar Rats ◽  
Smoking Behavior ◽  
Male Rats ◽  
Control Group ◽  
P Value ◽  
Adult Males ◽  
Control Group Design ◽  
Post Test ◽  
Male Wistar Rats

Background: Smoking is something that is usual for most Indonesian society, especially adult males. According to Riskesdas, smoking behavior of the population trends to increase. A previous study suggested that exposure to cigarette smoke produce CO bond to hemoglobin. Melon (Cucumis melo) contains antioxidants that prevent tissue damage.Objective: Analyze differences in levels HbCO Wistar male rats in treated and untreated extracts of melon.Method: The research was a laboratory experiment with the post test control group design and complete random design. The subjects were 25 male Wistar rats aged 3 months. Subjects were divided into 5 groups and each group received treatment for 28 days. Data of level HbCO was collected and analyzed by Anova One Way test at 95% confidence level.Results: The results showed a difference between the average levels of HbCO male Wistar rats (p value=0,000) treated and untreated extracts of melon.Conclusion: Melon extracts effect on decreasing the levels of HbCO due to exposure to cigarette smoke. There were differences in levels of male Wistar rats HbCO on treated and untreated extracts of melon and be given at a dose of 250 IU/day.

scholarly journals Supplementation of Red Bean (Phaseolus vulgaris L) Extract Decreased Malondialdehyde (MDA) Levels of Stressly Induced Activity of Male Wistar Rats (Rattus norvegicus)

2019 ◽  
Vol 6 (2) ◽  
pp. 262
Author(s):  
Gina Septivani ◽  
Wimpie Pangkahila ◽  
Indraguna Pinatih
Keyword(s):  
Phaseolus Vulgaris ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Positive Control ◽  
Control Group ◽  
Phaseolus Vulgaris L ◽  
Control Group Design ◽  
Post Test ◽  
Male Wistar Rats ◽  
Red Bean

This study aims to prove that supplementation of  red bean extract (Phaseolus vulgaris L) decreased malondialdehyde (MDA) levels of stressly induced activity of male wistar rats (Rattus norvegicus). This research was an experimental with pre-test post-test control group design. The rats were randomly divides into 3 groups: negatif control group (P0), positive control group (treated with overtraining and aquades 2ml/P1), and treatment group (treated with supllementation of red bean extract 650 mg/200 g body weight and overtraining/P2). The rats were swam for 45 minutes everyday. Data were analysed by one way anova test. The study showed that MDA level pretest of the each group was 69,70±0,52 mmol/L; 70,12±0,54 mmol/L and 69,59±0,51 mmol/L respectively. MDA level posttest of the each group was 69,66±0,27 mmol/L; 90,08±0,20 mmol/L and 62,85±0,50 mmol/L respectively. From the result of this study, it can be concluded that suplementation of red bean extract (Phaseolus vulgaris L) decreased malondialdehyde (MDA) levels of stressly induced activity of male wistar rats (Rattus norvegicus). Keyword: red bean (Phaseolus vulgaris L) extract, rats, overtraining, MDA, stress oxidative.

scholarly journals THE EFFECT OF PANORAMIC RADIOGRAPHY ON THE NUMBER OF MICRONUCLUES IN PERIODONTITIS

2021 ◽  
Vol 6 (2) ◽  
pp. 141
Author(s):  
Khairunnisa Puspita Sari ◽  
Didit Aspriyanto ◽  
Beta Widya Oktiani
Keyword(s):  
Dna Damage ◽  
Wistar Rats ◽  
Panoramic Radiography ◽  
Panoramic Radiograph ◽  
Normal Group ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Male Wistar Rats ◽  
Bonferroni Test

Background: Periodontitis is a periodontal disease that can cause an increase in Reactive Oxygen Species (ROS). The increase in ROS concentration will cause oxidative stress which has a role in DNA damage. In the case of periodontitis, panoramic radiography plays an important role in showing generalized horizontal bone loss. Radiation on panoramic radiography may cause DNA damage. DNA damage that occurs due to periodontitis or panoramic radiographic radiation exposure is characterized by the formation of micronuclei in gingival epithelial cells. Objective: To determine the effect of panoramic radiography on the number of micronuclei in Wistar rats with periodontitis. Method: This study was true experimental with post-test only and control group design. This study used 15 male Wistar rats which divided into 5 groups. Result: The average number of micronuclei in the normal group and 1 time exposures was 4 compared to the periodontitis group without exposure was 1.67, periodontitis and 1 time exposures was 8, periodontitis and 2 times the exposure was 15.67, and in the periodontitis and 3 times the exposure was 42.67. Result of One-Way Annova test and Post Hoc Bonferroni test indicated that signigicant changes in the number of micronuclei was seen between the normal group with 1 time exposure to the periodontitis group with 2 times and 3 times exposure and int the periodontitis group without exposure to the periodontitis group with 2 times and 3 times exposure. Conclusion: Panoramic radiograph X ray radiation and periodontitis can cause changes in the number of micronuclei in wistar rats. Keywords: , Micronucleus, Panoramic Radiography, Periodontitis.

scholarly journals The potential of red dragon fruit peel yogurt to improve platelet levels in heparin-induced thrombocytopenia in Wistar rats

10.5219/1497 ◽  
2021 ◽  
Vol 15 ◽  
pp. 218-225
Author(s):  
Widya Hary Cahyati ◽  
Nur Siyam ◽  
Natalia Desy Putriningtyas
Keyword(s):  
Wistar Rats ◽  
Control Group ◽  
Fruit Peel ◽  
Control Group Design ◽  
Treatment Groups ◽  
Dragon Fruit ◽  
Antioxidant Function ◽  
Before And After ◽  
Post Test ◽  
Male Wistar Rats

Patients infected with the dengue virus will develop thrombocytopenia which can cause bleeding and complications. One of the materials that contain antioxidants and have potential as a functional food is red dragon fruit peel. This peel can be processed into yogurt as a way to increase antioxidant function which ultimately supports the immune system of its users. This study analyzed the effect of red dragon fruit peel yogurt on the platelet levels of thrombocytopenic Wistar rats. It used a pre-post-test control group design. Male Wistar rats were randomly assigned into seven groups: K-; K+; and five treatment groups that received dragon fruit peel yogurt at doses of 5% (K1); 10% (K2); 15% (K3); 20% (K4); and 25% (K5). Thrombocytopenia was induced by 0.1 mL.100g-1 BW of heparin for 3 days. The intervention was carried out for 28 days. The result showed that all groups had significant differences before and after the intervention (p <0.05). Tukey analysis showed that there were significant differences in all groups (p <0.05). Yogurt containing 25% red dragon fruit peel provides an effective dose for improving platelet levels in thrombocytopenic rats.

scholarly journals Pemberian oral ekstrak kulit buah lemon (Citrus limon) menghambat peningkatan ekspresi MMP-1 (matrix metaloproteinase-1) dan penurunan jumlah kolagen pada tikus putih galur wistar jantan (Rattus norvegicus) yang dipajan sinar UV-B

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Ahmad M. Anshori ◽  
Anak A.G.P. Wiraguna ◽  
Wimpie Pangkahila
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Ultraviolet B ◽  
Control Group ◽  
Control Group Design ◽  
Lemon Peel ◽  
Post Test ◽  
Male Wistar Rats ◽  
Per Oral ◽  
Peel Extract

Abstract: Ultraviolet B (UVB) is a source of free radicals that accelerate aging process of the skin such as activating enzymes that degrade collagen and inhibit collagen production by inducing the expression of MMP-1. Lemon peel contains vitamin C, vitamin A, tannins and phenols which possess antioxidant activity and prevent oxidative stress. This study was aimed to prove that oral administration of lemon peel extract could decrease MMP-1 levels and increase the number of collagen in the UVB-induced male Wistar rats (Rattus norvegicus). This was a true experimental study with the post test only control group design. Subjects were 30 male Wistar rats (Rattus norvegicus), aged 2-3 months, body weight 120 g, divided into 3 groups, as follows: the control group (P0) which were not exposed to UVB; the treatment group 1 (P1) given aquabidest and UVB ray exposure; and the treatment group 2 (P2) given lemon peel extract and UVB ray exposure. After 15 days of treatment, all rats were anesthetized and their skin tissues were prepared for histological examination of MMP1 and collagen. The results showed that the average expression of MMP1 in P0 group was 22.02±3.20%; in the P1 group was 29.04±6.36%; and in P2 group was 7.98±2.76% (P <0.01). In addition, the average amount of collagen in P0 group was 70.01±2.99%; in the P1 group was 57.68±4.84%; and in P2 group was 77.45±4.29% (P <0.01). Conclusion: Oral administration of lemon peel extract could decrease the expression of MMP-1 and increase the amount of collagen in the UVB-induced male Wistar rats (Rattus norvegicus).Keywords: lemon peels, collagen, MMP-1, UVB Abstrak: Ultraviolet B (UVB) merupakan sumber radikal bebas yang mempercepat proses penuaan kulit dengan mengaktivasi enzim yang mendegradasi kolagen dan menghambat produksi kkolagen melalui induksi ekspresi MMP-1. Kulit buah lemon mengandung vitamin C, vitamin A, tanin, dan fenol yang memiliki aktivitas antioksidan dan mencegah stres oksidatif. Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak kulit buah lemon per oral dapat menurunkan kadar MMP-1 dan meningkatkan jumlah kolagen pada tikus putih jantan galur Wistar (Rattus norvegicus) yang dipajan sinar UVB. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subjek penelitian ialah 30 ekor tikus putih jantan, galur Wistar (Rattus norvegicus), umur 2-3 bulan, dengan berat badan 120 gr yang dibagi menjadi 3 kelompok, masing-masing berjumlah 10 ekor tikus. Kelompok kontrol (P0) tidak dipapar sinar UVB; kelompok perlakuan 1 (P1) diberikan aquabides oral dan paparan sinar UVB; dan kelompok perlakuan 2 (P2) diberikan ekstrak kulit buah lemon oral dan paparan sinar UVB. Setelah 15 hari perlakuan, seluruh tikus dianestesi kemudian diambil jaringan kulitnya untuk dibuat preparat histologik dan dihitung jumlah kolagen dermisnya sebagai data post test. Hasil analisis menunjukkan rerata jumlah ekspresi MMP1 pada kelompok P0 ialah 22,02±3,20%; kelompok P1 ialah 29,04±6,36%; dan kelompok P2 ialah 7,98±2,76% (P <0,01). Rerata jumlah kolagen pada kelompok P0 ialah 70,01±2,99%; kelompok P1 ialah 57,68±4,84%; dan kelompok P2 ialah 77,45±4,29% (P <0,01). Simpulan: Pemberian ekstrak kulit buah lemon per oral dapat menurunkan ekspresi MMP-1 dan meningkatkan jumlah kolagen pada tikus putih jantan galur Wistar (Rattus norvegicus) yang dipajan sinar UVB. Kata kunci: kulit buah lemon, kolagen, MMP-1, UVB

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