scholarly journals Biologically active and reserve substances of Siberian peonies

2021 ◽  
Vol 38 ◽  
pp. 00061
Author(s):  
Vera A. Kostikova ◽  
Olga V. Kalendar ◽  
Nikolai A. Tashev ◽  
Andrey S. Erst ◽  
Olga Yu. Vasilyeva
Keyword(s):  
Ascorbic Acid ◽  
Biologically Active ◽  
Growth Season ◽  
Siberian Species

Levels of biologically active and reserve substances were investigated in the leaves and rhizomes of Paeonia anomala L., P. hybrida Pall., and P. lactiflora Pall. The peonies’ leaves are rich in ascorbic acid (1205.7 mg/100g in P. anomala) and tannins (15.9% in P. lactiflora and 15.7% in P. anomala) at the beginning of the growth season. Concentrations of flavonols, pectins, and carotenoids in the Siberian peonies are low at the beginning of the growth season. P. lactiflora is a promising species for further research on the set and levels of biologically active and reserve substances in rhizomes because this species stands out among the three Siberian species in terms of the levels of catechins (1297.8 mg/100g), protopectins (10.1%), saponins (19.2%), and sugars (22.1%) in rhizomes. In these parts of the plant, starch (18.6%) accumulates the most in P. hybrida among the studied peonies species. Concentrations of the analyzed substances in the rhizomes of the peonies change significantly by the end of the growth season and vary widely among these species. Levels of ascorbic acid (except for P. hybrida), protopectins, and starch increase significantly while concentrations of catechins and sugars decrease in the rhizomes by the end of the growth season.

scholarly journals Gas Separation Membranes upon Production of Functional Berry Juice

2019 ◽  
Vol 9 (1) ◽  
pp. 6616-6619
Keyword(s):  
Ascorbic Acid ◽  
Gas Separation ◽  
Biologically Active ◽  
Juice Quality ◽  
Active Components ◽  
Gas Separation Membrane ◽  
Separation Membranes ◽  
Gas Separation Membranes ◽  
Separation Membrane ◽  
New Generation

Nowadays manufacturing of new-generation food, including functional products, is especially important. Drinks based on fruits and berries are considered at present as optimum form of food required for organism replenishment with biologically active components and vitamins aiming at organism recovery. The retention of valuable components of fruits and berries is of great concern during such production. In order to produce functional juices, it is required to decrease oxygen amount upon their production in order to prevent destructive changes in drink, to improve its quality, to perform subsequent procedures and preservation. It is proposed to modify deaeration and to apply advanced equipment: deaerator with gas separation membrane which would allow to remove higher oxygen amount in comparison with the existing equipment while producing juices of predefined quality with high content of vitamins. The experiments were carried out with freshly squeezed black current juices using a vacuum unit, an Expert-009 analyzer of dissolved oxygen with optical sensor, a gas separation membrane. The juice quality was determined by its content of ascorbic acid (vitamin C) since it is destroyed under impact of oxygen. The content of ascorbic acid during storage was measured on juices produced by different deaeration procedures. The obtained experimental results proved efficiency of membrane application during juice production showing high content of vitamins. The content of ascorbic acid during storage was higher in the juice after deaeration using gas separation membrane in comparison with the juice without treatment and with the juice after vacuum deaeration. Therefore, the most efficient method to achieve the best removal of oxygen from juice is preheating of juice to 65°C with subsequent application of deaerator with gas separation membrane, subsequent packing and cooling of final product.

scholarly journals Flowers of Campanula species as a source of biologically active substances

2021 ◽  
Vol 38 ◽  
pp. 00033
Author(s):  
Tatiana Fomina ◽  
Tatiana Kukushkina
Keyword(s):  
Ascorbic Acid ◽  
Western Siberia ◽  
Food Additives ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Forest Steppe ◽  
Wet Weight ◽  
First Time ◽  
Active Substances

The content of the major groups of biologically active substances in flowers of 7 Campanula species, cultivated in the forest-steppe of Western Siberia, were investigated for the first time. Freshly collected flowers were found to contain 0.06-0.15 % of catechins, 0.31-3.17 % of flavonols, 3.31-6.47 % of tannins, 3.10-15.57 % of pectic substances, and 0.66-16.4 mg% of carotenoids calculated per absolute dre weight of raw material. The amount of ascorbic acid was 36.9-114.0 mg% per wet weight. The data indicate that Campanula flowers are promising for the production of phenolic compounds, the development of food additives and fresh consumptions a seasoning.

scholarly journals Association between Hydrogen Peroxide-Dependent Byproducts of Ascorbic Acid and Increased Hepatic Acetyl-CoA Carboxylase Activity

2005 ◽  
Vol 51 (8) ◽  
pp. 1462-1471 ◽  
Author(s):  
Laurent Knafo ◽  
Philippe Chessex ◽  
Thérèse Rouleau ◽  
Jean-Claude Lavoie
Keyword(s):  
Ascorbic Acid ◽  
Lipid Metabolism ◽  
Guinea Pig ◽  
Dehydroascorbic Acid ◽  
Biologically Active ◽  
Urinary Concentration ◽  
Acetyl Coa Carboxylase ◽  
High Concentration ◽  
Acetyl Coa ◽  
Carboxylase Activity

Abstract Background: Parenteral multivitamin preparation (MVP) induces fatty liver in neonatal guinea pig pups; this is prevented by photoprotection. Photo-excited riboflavin present in MVP generates H2O2 and molecules with masses of 136 and 208. We hypothesized that H2O2 initiates the peroxidation of ascorbic acid (AA), producing biologically active byproducts affecting hepatic lipid metabolism. Methods: Mass spectrometry (MS) documented the participation of H2O2 and photo-excited riboflavin (Ribo) in the formation of AA byproducts. Sixteen 3-day-old guinea pig pups received an intravenous solution (50 g/L dextrose + 4.5 g/L NaCl + 1 kIU/L heparin) at 240 mL · kg−1 · day−1, enriched with control or test mixtures, for 4 days. The control mixture was photo-protected AA + Ribo (without byproducts or H2O2), and the test mixture was AA + Ribo treated to generate AA byproducts without H2O2. Hepatic acetyl-CoA carboxylase (ACC) activity was determined after 4 days. Fourth-day urine samples were analyzed by MS. Data were treated by ANOVA (α = 0.05). Results: H2O2 did not influence the classic degradation of AA, as the generation of 2,3-diketogulonic acid was not affected. In contrast, the formation of molecules with masses of 136 and 208 was H2O2 and time dependent. ACC activity was higher (P <0.01) in animals receiving high concentration of these molecules; its hepatic activation correlated (P <0.01) with the urinary concentration of molecule-208. Conclusions: H2O2 at concentrations found in the clinical setting of total parenteral nutrition induce the transformation of dehydroascorbic acid into compounds that have the potential to affect lipid metabolism. These molecules have peroxide and aldehyde functions.

scholarly journals Evaluation of strawberry varieties by biochemical composition and commercial qualities under the conditions of Orenburg Urals

2019 ◽  
pp. 26-34 ◽  
Author(s):  
A. A. Mushinsky ◽  
E. V. Aminova ◽  
Z. A. Avdeeva ◽  
T. A. Tumaevа ◽  
A. A. Borisova ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Comparative Evaluation ◽  
Weather Conditions ◽  
Commercial Production ◽  
Biologically Active ◽  
Soluble Solids ◽  
Average Content ◽  
The Urals ◽  
Experimental Station

The article presents the results of studies of the chemical composition, commercial qualities and taste of berries of promising strawberry varieties breeding of the All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery (Moscow) in the conditions of the Orenburg Urals. The studies were carried out in 2016-2018 on the basis of the Orenburg Experimental Station of Horticulture and Viticulture of the All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery in specific conditions, where almost annually dry and moderately dry climatic periods were observed during the formation of the yield (May-June). A comparative evaluation of varieties on the content of sugars, acids, vitamin C in berries, taste and attractiveness of appearance is given. The dependence of the studied parameters on the genotype of the variety and vegetation conditions was established. Depending on the variety and year, the content of soluble solids varied from 7.2 to 10.2 %, with an average content of 8.9 %; total acidity varied from 0.8 to 1.2 %, with an average content of 1.0 %; the content of ascorbic acid (vitamin C) was from 45.1 to 78.5 mg/100 g, with an average content of 56.4 mg/100 g. Varieties with high parameters of tasting evaluation (Darenka, Kokinskaya Zarya, Studencheskaya), soluble solids (Rosinka, Studencheskaya) and ascorbic acid (Kokinskaya Zarya, Bereginya) were revealed. They are recommended to be grown to obtain fresh consumption products and as processing products of various directions. Bereginya, Darenka and Rosinka varieties surpass control varieties in certain biochemical parameters and can be used in breeding in the Urals region to obtain new genotypes with an increased content of a specific biologically active substance. The variety Tsaritsa is selected for high commodity qualities of berries (weight, dessert taste, attractiveness of appearance) and is recommended for commercial production of berries and involvement in breeding process. It has been established that the taste of berries is determined by a complex of acids, sugars and aromatic substances. This parameter was greatly influenced by weather conditions, from which air temperature and precipitations were determining.

scholarly journals Determination of the comprehensive indicator of pastille with the use of multi-component fruit-berry paste

2021 ◽  
Vol 3 (3(59)) ◽  
pp. 46-49
Author(s):  
Kateryna Kasabova ◽  
Nataliа Shmatchenko ◽  
Aleksey Zagorulko ◽  
Andreii Zahorulko
Keyword(s):  
Ascorbic Acid ◽  
Chemical Composition ◽  
Raw Materials ◽  
Control Sample ◽  
Comprehensive Assessment ◽  
Biologically Active ◽  
Human Need ◽  
Pectin Substances

The object of research in this work is the technology of pastille with the addition of multicomponent fruit and berry paste. Pastille are becoming more and more popular in the world for all segments of the population. Usually, the main ingredients for their manufacture are fruit raw materials, a structurant and egg white, therefore, the products contain practically no fat and have a low calorie content. It is proposed to replace 75 % of traditional applesauce in the recipe with a multicomponent fruit and berry paste made from apples, cranberries, hawthorn. It was found that such a composition makes it possible to obtain products with an increased content of pectin substances, ascorbic acid, polyphenols, including anthocyanins, catechins and flavonols. The work is aimed at studying the application of the principles of qualimetry to determine a complex indicator of the quality of pastille For this, the organoleptic and physicochemical indicators of the quality of the developed pastille were taken from previous studies and the chemical composition was determined and the content of biologically active substances was calculated relative to the average daily human need. The content of pectin substances in new products increases 1.7 times and satisfies the average daily human need by 18.2 %. The content of ascorbic acid is 4.2 times higher, which is 20.5 % of the average daily human need, and polyphenols 3.2 times higher – 579.0 %. The data obtained make it possible to consider the developed product functional. The next step was to determine the complex indicator of products by the qualimetric method. For a more complete disclosure of product properties, a structure of pastille quality indicators has been developed – a «tree of properties». Thus, the comprehensive assessment of the quality of the control sample corresponds to the indicator «good» (0.66), while the integrated assessment of pastille with multicomponent paste corresponds to the indicator «very good» (0.98). On the basis of organoleptic, physicochemical indicators and chemical composition of products, it was established that the indicator of a comprehensive assessment of the quality of a pastille with a multicomponent paste, taking into account all group indicators, is 48 % higher compared to the control.

scholarly journals Role of antioxidants in electro catalysis of cytochrome P450 3A4

2014 ◽  
Vol 60 (2) ◽  
pp. 224-234 ◽  
Author(s):  
V.V. Shumyantseva ◽  
A.A. Makhova ◽  
T.V. Bulko ◽  
E.V. Shich ◽  
V.G. Kukes ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Catalytic Activity ◽  
Vitamin E ◽  
Vitamin A ◽  
Antioxidant Properties ◽  
Biologically Active ◽  
Catalytic Current ◽  
Electrochemical Approach ◽  
Electro Catalysis ◽  
Clinical Experiments

The electrochemical analysis of cytochrome Р450 3А4 catalytic activity has shown that vitamins C, A and Е influence on electron transfer and Fe3+/Fe2+ reduction process of cytochrome Р450 3А4. These data allow to assume possibility of cross effects and interference of vitamins-antioxidants with drugs metabolised by cytochrome Р450 3А4, at carrying out of complex therapy. This class of vitamins shows antioxidant properties that lead to increase of the cathodic current corresponding to heme reduction of this functionally significant haemoprotein. Ascorbic acid of 0.028-0.56 mM concentration stimulates cathodic peak (an electrochemical signal) of cytochrome Р450 3А4. At the presence of diclofenac (Voltaren) - a typical substrate of cytochrome Р450 3А4 - the increase growth of a catalytic current testifying to an electrocatalysis and stimulating action of ascorbic acid is observed. In the presence of vitamins A and Е also is registered dose-dependent (in a range of 10-100 M) increase in a catalytic current of cytochrome Р450 3А4: the maximum increase corresponds to 229 ± 20% for 100 M of vitamin A, and 162±10% for 100 M of vitamin E. Vitamin E in the presence of P450’s inhibitor itraconazole doesn't give essential increase in a reductive current, unlike retinol (vitamin A). This effect can manifest substrate properties of tocopherol (vitamin E). The electrochemical approach for the analysis of catalytic activity of cytochrome Р450 3А4 and studies of influence of biologically active compounds on an electrocatalysis is the sensitive and effective sensor approach, allowing to use low concentration of protein on an electrode (till 10-15 mol/electrode), to carry out the analysis without participation of protein redox partners, and to reveal drug-drug or drug-vitamins interaction in pre-clinical experiments.

scholarly journals Antimicrobial Activity Exerted by Total Extracts of Germander

2019 ◽  
Vol 70 (9) ◽  
pp. 3242-3244
Author(s):  
Raul Chioibas ◽  
Razvan Susan ◽  
Monica Susan ◽  
Ovidiu Mederle ◽  
Delia Berceanu Vaduva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Fungal Species ◽  
Polyphenolic Compounds ◽  
Biologically Active ◽  
E Coli ◽  
Methanolic Extracts ◽  
Teucrium Polium ◽  
Active Substances

In the present research, ethanolic and methanolic extracts of Teucrium polium (germander) were studied regarding the content of biologically active substances - in particular polyphenols - antioxidant activity and antibacterial activity. The extracts have been found to be rich in polyphenolic compounds, kaempferol and epichatechin have been found to be present in the highest concentrations. The antioxidant activity evaluated by the method of capturing the free radicals with DPPH revealed values close to those produced by the ascorbic acid. Antimicrobial activity was evaluated by the diffusimetric method and S. aureus and E. coli microorganisms were found to be the most sensitive while P. aeruginosa and fungal species were not sensitive.

scholarly journals Application of the Agrimonia eupatoria L. Extract for the Kvases Production

Food Industry ◽  
2020 ◽  
Vol 5 (3) ◽  
pp. 35-43
Author(s):  
Yuliya Miller ◽  
Olga Golub ◽  
Karina Zakharova
Keyword(s):  
Ascorbic Acid ◽  
Test Methods ◽  
Quality Characteristics ◽  
Maximum Amount ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Chemical Parameters ◽  
Organoleptic Characteristics ◽  
Physical And Chemical ◽  
Active Substances

The article concerns the research of the herb Common Agrimony (Agrimonia eupatoria L.) use in the kvass manufacture, because the plant has a high content of flavoring and biologically active substances (flavonoids, tannins, arbutin, etc.). Researchers used standard, adopted in the brewing and soft drinks industry test methods and methods of processing obtained results. To produce kvass with the best quality characteristics, a man has to use Agrimonia eupatoria L. extract. In order to obtain this extract with the maximum amount of flavoring and biologically active substances (polyphenolic substances, ascorbic acid) a man must macerate 3-5 mm cut grass using purified water as a solvent in a ratio of 1:10 at a temperature of 40 ± 3 °C for 5-6 hours. Agrimonia eupatoria L. extract is an opaque brown liquid with a weak greenish tint, weakly expressed sweet-apricot aroma and taste; the mass fraction of dry substances is 3.0 ± 0.1 %; acidity – 2.5 ± 0.2 units; the content of polyphenolic substances is not less than 13.5 mg / 100 cm3; the content of ascorbic acid – not less than 4.5 mg / 100 cm3 . The authors developed kvass technology, including the preparation of kvass wort and Agrimonia eupatoria L. extract separately, its blending, fermentation, cooling, clarification and bottling. Based on the organoleptic and physical and chemical parameters obtained during the study, the researchers recommended the optimal ratio of kvass wort and Agrimonia eupatoria L.: 90:10 – 80:20. They developed regulated quality indicators of the new kvass: appearance – a transparent foaming liquid with opalescence, without foreign inclusions; color – brown with greenish tones; aroma and taste – refreshing, sweet and sour, bread, with light tones of Agrimonia eupatoria L. extract. The use of an aqueous extract from the herb Agrimonia eupatoria L. in the kvass production enables to get products with original organoleptic characteristics

scholarly journals EFFECT OF BIOLOGICALLY ACTIVE PREPARATIONS ON PRODUCTIVITY AND QUALITY OF SWEET PEPPER FRUITS IN CONTROLLED CONDITIONS

2018 ◽  
pp. 81-85
Author(s):  
O. R. Udalova ◽  
V. N. Pishchik ◽  
G. V. Mirskaya ◽  
V. Eu. Vertebny ◽  
N. I. Vorobyov ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Bacillus Subtilis ◽  
Growth Promotion ◽  
Sugar Content ◽  
Sweet Pepper ◽  
Biologically Active ◽  
Average Weight ◽  
Controlled Conditions ◽  
Joint Application

Effects of humic preparation (HP) Stimulife and PGPR (plant growth promotion rhizobacteria) Bacillus subtilis №2 on the productivity and quality of the sweet pepper (Capsicum annuum L.) fruits were studied in the control conditions. It was experimentally established that the use of Stimulife and bacteria Bacillus subtilis №2 resulted in an increase in plant height, productivity and biochemical composition of sweet peppers. Differences in the effect of the studied preparations on the morphometric, phenological and biochemical characteristics of plants and fruits of pepper varieties Ermak and Cinderella were revealed. The bacteria Bacillus subtilis №2 significantly increased the yield, the number of fruits per one plant and the contents of ascorbic acid and carbohydrates in pepper fruits. In this case, the average weight of one fruit of pepper v. Ermak decreased. HP Stimulife increased the pepper yield due to increasing the mean mass of one fruit in both pepper varieties. Joint used of HP Stimulife and bacteria Bacillus subtilis №2 led to an intensification of the production process of plants compared to the experiment variants, in which HP Stimulife was used alone. The yield of sweet pepper Ermak and Cinderella increased by 10-45%, the maturation period of fruits was reduced by 10-14 days, the content of ascorbic acid increased by 25%, sugar content increased to 26%. The expediency of the joint application of HP Stimulife and bacteria Bacillus subtilis №2 in the cultivation of sweet pepper under controlled conditions is substantiated.

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