scholarly journals Correcting physiological and biochemical status of service dogs with dihydroquercetin

2019 ◽  
Vol 135 ◽  
pp. 01074
Author(s):  
Galina Molyanova ◽  
Vladimir Ermakov ◽  
Irina Bistrova
Keyword(s):  
Antioxidant Defense ◽  
Antioxidant Properties ◽  
The Body ◽  
Similar Data ◽  
Service Dogs ◽  
Diene Conjugates ◽  
Adaptive Capabilities ◽  
Biochemical Status ◽  
Harmful Effects ◽  
Physiological And Biochemical

The search for new ways to optimize the adaptive capabilities of service dogs is a prerequisite for increasing the viability of animals and increasing their longevity. Dihydroquercetin neutralizes the increased level of free radicals toxic to the body, protecting cell membranes from destruction, and reduces the harmful effects of oxidative stress. The use of dihydroquercetin contributed to increased providing cells with oxygen, stimulated anabolic processes and increased the antioxidant defense of the body. The amount of red blood cells in the blood of service dogs increased by 11.5-19.1% (p<0.01) when using dihydroquercetin, hemoglobin increased by 5.7-9.4% (p<0.05), total protein by 21-32% (p<0.01), albumin by 30-31% (p<0.001), compared with similar data at the beginning of the experiment. The antioxidant properties of dihydroquercetin contributed to the reduction of malondialdehyde by 23.9% (p <0.05); reduction of diene conjugates by 26%; and an increase in catalase activity by 36% compared to the data on the first day of the experiment. The use of dihydroquercetin optimizes the adaptive capabilities of service dogs and can be used to increase the vitality and longevity of animals.

scholarly journals The study of the antioxidant activity of polyphenol extracts from cranberry leaves on the model of insulin resistance

2021 ◽  
pp. 23-27
Author(s):  
O. Krasilnikova ◽  
G. Kravchenko ◽  
O. Shovkova
Keyword(s):  
Insulin Resistance ◽  
Antioxidant Defense ◽  
Antioxidant Properties ◽  
Sharp Decrease ◽  
The State ◽  
The Body ◽  
Male Rats ◽  
Diene Conjugates ◽  
Polyphenolic Extract ◽  
Number Of Patients

Topicality. In Ukraine, the number of patients with diabetes is growing rapidly, it ranks the 4th in the structure of diseases of the endocrine system, and it is one of the main causes of incapacitation and disability among the population and takes the second place by the number of deaths. Polyphenolic extracts from leaves of the heather (Ericaceae) family, in particular blueberries, cranberries and bearberry, in the previous studies demonstrated the presence of antioxidant, hypoglycemic and lipotropic properties. However, the pharmacological activity of extracts from cranberry leaves has not been sufficiently studied. Aim. To study the effect of polyphenolic extracts from cranberry leaves on the processes of LPO and the state of the antioxidant defense in the liver and serum in rats with the experimental insulin resistance. Materials and methods. The study was performed on white outbred male rats weighing 180 ± 15 g, kept in the vivarium of the NUPh. Insulin resistance was induced by keeping animals on a high fructose diet for 6 weeks according to the recommendations. The extracts were administered intragastrically in the dose of 200 μg/100 g of the body weight from the fourth week of the experiment for 14 days. The POL activity was assessed by the level of TBA reactants, diene conjugates and conjugated trienes in the liver and serum. The state of the antioxidant defense was assessed by the level of reduced glutathione, as well as the activity of catalase and superoxide dismutase. Indicators were determined by the spectrophotometric method. Results and discussion. The development of the experimental resistance was accompanied by an increase in LPS activity in the liver and serum of rats. In the serum of animals with IR the content of primary products of LPO increased in 1.75 times, the content of triene conjugates in 1.7 times, respectively, while the content of TBA reactants in 1.61 times. The introduction of the polyphenolic concentrate from cranberry leaves was accompanied by a sharp decrease in the products of lipid peroxidation both in the eserum and liver to almost ascending levels. There was normalization of the activity of enzymes of the first line of the antioxidant defense compared to animals with insulin resistance due to the presence of plant flavonoids in the extract. Conclusions. Thus, the data obtained indicate that the polyphenolic extract from cranberry leaves exhibits antioxidant properties in the liver and serum of rats with the experimental insulin resistance. The polyphenolic extract also restored the antioxidant defense of cells and the body as a whole. The data obtained indicate the need for further research to create a new herbal medicine for use in the treatment of insulin resistance and diabetes.

scholarly journals Activity of the antioxidant defense system of the carp organism under the influence of stress factors and the use of milk thistle in feeding

2021 ◽  
Author(s):  
I. I. Hrytsyniak ◽  
◽  
O. V. Deren ◽  
M. Z. Korilyak ◽  
R. A. Palamarchuk ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Antioxidant Defense ◽  
Antioxidant Properties ◽  
Fish Farming ◽  
Antioxidant Defense System ◽  
Stress Factors ◽  
Milk Thistle ◽  
Defense System ◽  
Diene Conjugates ◽  
Growing Conditions

Intensification measures in carp farming are accompanied by the emergence of stressors, which negatively affects the productive characteristics and functional state of organs and systems of fish. One of the ways to counteract their negative effects on the body is to stimulate the antioxidant defense system. The aim of the study is to analyze the effectiveness of plant additives with antioxidant properties of milk thistle in carp feeding, under optimal housing conditions (Experiment I) and under the influence of stress factors common in fish farming, namely: pollution of the aquatic environment with nutrients (Experiment 2) and this pollution. complexes with a decrease in the content of dissolved oxygen in water (Experiment 3). The studies used one-year-old carp, which were divided into experimental and control groups within each of the three variants of the experiment. Control groups of fish received feed with a protein content of 23%. Carp of the experimental groups in addition to the feed by granulation was introduced crushed seeds of milk thistle in the amount of 5%. Experimental feeding lasted 20 days. In accordance with the generally accepted methods and purpose of the experiment in fish farming, the physicochemical parameters of the aquatic environment are provided. Indicators of lipid peroxidation (LPO) and activity of the body's antioxidant defense system (ANS) were analyzed. It was determined that when feeding thistle carp under annual conditions under optimal growing conditions in the muscles of the experimental group there is some tendency to increase the content of catalase (by 14.6%), and the content of SOD, TBC products and diene conjugates is at the same level as and in control. In the hepatopancreas, on the other hand, the level of catalase decreases somewhat relative to control and there is a tendency to reduce TBA products and the content of diene conjugates is reduced by 75% (p <0.05). When feeding milk thistle for contamination of the aquatic environment with nutrients in the muscles almost three times the content of SOD (p˂0.01). The content of catalase tends to decrease, and the content of TBA products and diene conjugates increases by 51%. In the hepatopancreas there is a tendency to decrease all of the above indicators relative to the control group. It was found that the introduction of milk thistle in the feed under conditions of complex exposure to stressors in the muscles increases the content of SOD (p˂0,001). The content of catalase and TBA products decreases slightly in the hepatopancreas, the content of SOD (p˂0.001) and diene conjugates (p˂0.001) increases. Therefore, the antioxidant properties of milk thistle are more pronounced as a result of use in the feeding of carp in the presence of environmental stressors, compared with optimal growing conditions.

Antioxidant properties of ferulic acid and the main results of their implementation in the industrial incubation of turkeys

2021 ◽  
Vol 1 (1) ◽  
pp. 76-84
Author(s):  
T. O. Azarnova ◽  
◽  
A. M. Rezvykh ◽  
V. I. Maksimov ◽  
I. I. Kochish ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Ferulic Acid ◽  
Carbohydrate Metabolism ◽  
Old Age ◽  
Antioxidant Properties ◽  
Live Weight ◽  
The Body ◽  
Diene Conjugates ◽  
Turkey Poults ◽  
Experimental Group

The presented article presented data confirming the antioxidant properties of ferulic acid, which is associated with inhibition of the intensity of lipid peroxidation in the body of day-old turkeys, expressed in a decrease in the concentration of its cytotoxic products formed at different stages of the peroxide cascade: isolated double bonds (IDB) by 1,2 times, diene conjugates (DC) 1,6 times (p<0,001,.1,1), triene conjugates (TC) and oxodienic conjugates (ODC) 1,4 times, Schiff bases (SB) 12,02 times compared to the control. This helped to optimize the central metabolic processes, in particular carbohydrate metabolism. The paper analyzes these changes, presents and explains their relationship with the increase in quantitative and qualitative indicators of young turkeys at the early stages of ontogenesis. Thus, the superiority of the experimental group over the control of hatching turkey poults was 0,01% (p<6,66), the hatchability of eggs was 8,23% with a tendency to an increase in live weight of individuals of day-old age by 0,5%, the score according to the «Pasgar» scales and «Optistart» by 0,05 (p<1,0) and 0,001 points (p<XNUMX), respectively.

scholarly journals Details of the release of antistress properties of a biologically active iodine in a chicken embryos

2019 ◽  
pp. 58-63
Author(s):  
E. N. Indyuhova ◽  
T. O. Azarnova ◽  
V. I. Maksimov
Keyword(s):  
Mechanism Of Action ◽  
Health Workers ◽  
Medical Science ◽  
Antioxidant Properties ◽  
The Body ◽  
Biologically Active ◽  
Model Systems ◽  
Control Group ◽  
Diene Conjugates ◽  
Drug Mechanism

The physiological and biochemical effects of transovarial treatment by using biologically active iodine were examined with and without impact of extreme irritant on chicken and hen embryos which are the most popular model systems for investigations of the drug mechanism of action in the veterinary science and the medical science. The increasing functional activity of thyroid of chickens from an experienced team in the first days of life was investigated and analysed. The level of iodothyronines is higher in chickens under the action of heat stress factor: T3 total – 1.3 times, T3 free – of 20.9%, and T4 total”– of 23.2%, T4 free – 1.3 times compared with the control. There was an activation of the antioxidant defense system of the body in day-old chickens from the 2nd experimental group against the background of optimization of thyroid status, which resulted in a significant increase in the antioxidant activity of the blood serum compared to that in the control group 1.3 times, indicated a decrease in the level of: lipids, containing isolated double bonds – of 8.8%, diene conjugates – 1.4 times, triene conjugates - of 16.3%, oxodienic conjugates – 1.5 times and Schiff bases – 1.4 times. The central aspects of the iodothyronine mechanism of action at high intensity of free radical reactions and, consequently lipid peroxidation are presented in framework form. The results of the study will make possible the use of the drug products based on this chemical element for veterinarians and health workers. The more stressors affect the bird's body, the higher efficacy of the release of antioxidant properties is in thyroid hormones.

scholarly journals Effects of hydroalcoholic extract of Rosa damascena Mill on the cardiac angiogenesis in diabetic male rats

2020 ◽  
Vol 22 (2) ◽  
pp. 82-87
Author(s):  
Shiva Nosrati ◽  
Mohammad Zarei ◽  
Iraj Salehi ◽  
Sara Soleimani Asl ◽  
Alireza Komaki ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Defense ◽  
Antioxidant Properties ◽  
Serum Levels ◽  
Diabetic Rats ◽  
The Body ◽  
Male Rats ◽  
Rosa Damascena ◽  
Hydroalcoholic Extract ◽  
Significant Difference

Background and aims: The cardiac angiogenesis is one of the complications of diabetes. The oxidative stress occurs when there is an imbalance between the production of free radicals and antioxidant defense of the body. In diabetes mellitus, hyperglycemia induces increased oxidative stress through biological processes. Diabetes is also associated with reduced antioxidant defense by reducing glutathione, catalase, and superoxide dismutase. Rosa damascena Mill has antioxidant properties. This study aimed to investigate the effects of hydroalcoholic extract of R. damascena Mill on cardiac angiogenesis in diabetic rats. Methods: In this experimental study carried out in 2016, a total of 48 adult male Wistar rats (190-200 g) were randomly divided into 8 groups (n=6) including control, extracts (200, 400, and 1000 mg/kg), diabetic, and diabetic plus extracts (200, 400, and 1000 mg/kg). Diabetes was induced by intraperitoneal (i.p.) injection of streptozotocin (STZ) (50 mg/kg) and the rats were gavaged with different doses of extract for 30 days. At the end of the treatment, serum levels of vascular endothelial growth factor (VEGF) were determined by enzymelinked immunosorbent assay and the rat hearts were removed for immunohistochemistry study. Results: The treatment with hydroalcoholic extract of R. damascene Mill had no significant effect on cardiac capillary density [F (7, 40)=0.5, P>0.05] and the serum levels of VEGF [F (7, 40)=0.406, P>0.05]. However, there was no significant difference in blood glucose level in extract groups compared to control rats as well as diabetic plus extract groups compared to diabetic rats [F (7,40)=0.787, P>0.05]. Conclusion: According to our results, the hydroalcoholic extract of R. damascene Mill had no effect on cardiac angiogenesis in the normal and diabetic subjects. These findings were probably due to the insufficient amounts of effective ingredients, especially flavonoids, in this plant, or the duration of extract administration.

The use of water-soluble form of ubikhinone for leveling oxidative stress in chicken embryos as a method for optimizing the hematological status

2021 ◽  
Vol 1 (8) ◽  
pp. 51-61
Author(s):  
T. O. Azarnova ◽  
◽  
A. Yu. Sidorova ◽  
S. V. Pozyabin ◽  
I. S. Lugovaya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Properties ◽  
The Body ◽  
Water Soluble ◽  
Soluble Form ◽  
Negative Consequences ◽  
Hematological Indices ◽  
Adaptive Capabilities ◽  
Experimental Group ◽  
Artificial Incubation

The incubation eggs were treated with solutions of a water-soluble form of ubiquinone in order to neutralize the negative consequences of the development of oxidative stress in chick embryos under conditions of artificial incubation to optimize the hematological status in their body. The results showed that the implementation, first of all, of the antioxidant properties of the claimed metabolite made it possible to obtain the values of the central hematological indices, indicating a decrease in the body of individuals of the best experimental group of endogenous intoxication, an increase in stress resistance, adaptive capabilities and stabilization of homeostasis.

The Toxicity of Castor Beans and its Treatment with Doxycycline in Local Rabbits

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Nael Mohammed Sarheed ◽  
Osamah Faisal Kokas ◽  
Doaa Abd Alabas Muhammed Ridh
Keyword(s):  
Castor Bean ◽  
The Body ◽  
Control Group ◽  
High Concentration ◽  
Biochemical Tests ◽  
Doxycycline Treatment ◽  
High Concentrations ◽  
Physiological Tests ◽  
Animal House ◽  
Physiological And Biochemical

The plant of castor is widely spread in the Iraqi land, and characterized with containing ricin toxin, which has a very serious effects, and because the seeds of this plant scattered in the agricultural soil and rivers water, which increases the exposure of humans and animals to these beans. Objective: This experiment was designed to study the effect of high concentration of castor bean powder in some physiological and biochemical parameters and changes in some tissues of the body, as well as trying to use doxycycline to reduce the effects of ingestion of these seeds. Materials and Methods: In the experiment, 24 local rabbits were raised and fed in the Animal House of the Faculty of Medicine / Al-Muthanna University, then divided into four groups and treated for three weeks (21 days), Control group: treated with normal saline solution (0.9) orally throughout the experiment, G1: was treated orally with a concentration of 25 mg / kg of castor bean powder daily during the experiment, G2 : orally treated 25 mg / kg of castor bean and 25 mg / kg of doxycycline, G3: orally treated 25 mg / kg of castor powder with 50 mg / kg of doxycycline daily throughout the trial period. Results: The results of the experiment showed significant changes (P less than 0.05) in all physiological and biochemical blood tests when compared with control group. There was a significant decrease in PCV, Hb, RBC, T.protein and body weights, while demonstrated a significant increase in WBC, Urea, Creatinine, ALT, AST and ALP, with distortions in liver and kidney of animals that treated with Castor beans. In contrast, the treatment with doxycycline and caster beans showed significant improvement reflected by a normal proportion in physiological tests and biochemical tests with improvement in the tissues when compared to control group. Conclusions: It can be concluded from this study that castor bean has high toxic and pathogenic effects that may be dangerous to the life of the organism. Therefore, it is advisable to be cautious of these pills and avoid exposure to them, also recommended to take high concentrations of doxycycline treatment when infected with castor bean poisoning.

Indicators of metabolism and biochemical status of blood in horses of Yakut breed in winter and spring seasons

2020 ◽  
pp. 52-65
Author(s):  
U. Khompodoeva ◽  
R. Ivanov
Keyword(s):  
Classical Method ◽  
Live Weight ◽  
Weather Conditions ◽  
The Body ◽  
Metabolic Energy ◽  
Temperature Changes ◽  
Experimental Animals ◽  
Spring Period ◽  
Biochemical Status ◽  
Adaptive Mechanisms

The results of researches on the peculiarities of metabolism in the body of horses of Yakut breed in the winter and spring periods under the environments of Central Yakutia have been presented in the article. Physiological experiments on the digestibility of the main nutrients of the feed have been carried out in the winter (February) and spring (April) periods under the environments of the physiological yard in the Yakutsk Scientific and Research Institute of Agriculture named after M. G. Safronov with using the classical method. Five mares and five geldings of Yakut breed have been selected for researches. The average live weight of mares was 378–410 kg, geldings 394–403 kg. The experimental animals had average fatness. It has been found a higher intake of energy and the main nutrients of hay as a mono feed in winter compared to the spring period. At the same time pregnant mares in winter have been consumed 103,8±3,12 MJ of metabolic energy per head/day, which was 27,3 % significantly higher than in the spring period – 75,4±2,18 MJ (P ≥ 0,999). Geldings have been consumed in the experiment 67,70±2,85 MJ, which was by 43,7 % higher than in the spring period – 38,05 ± 4,40 MJ of metabolic energy per head/day (P ≥ 0,999). Experimental animals have been housed in stalls; there was no energy expenditure for movement, so the amount of energy consumed in winter indicates the production of heat in the animal’s body. Pregnant mares per 100 kg of live weight have consumed 31,5±4,11 MJ of metabolic energy or 33,3 % more than in the spring period – 21,0±1,98 MJ; geldings have consumed 27,69±1,12, which was 41,78 % significantly higher than in the spring – 16,12 ± 0,75 MJ (P ≥ 0,999). The high intake of metabolic energy into the body of Yakut horses in winter is explained by the manifestation of adaptive mechanisms that affect the effective metabolism for survival, adapted to frequent changes in weather conditions and sharp temperature changes. The established differences in the biochemical parameters of blood serum of horses of Yakut breed are associated with changes in the consumption and digestibility of basic nutrients and energy, depending on the ambient temperature.

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