scholarly journals Effect of Yellow Propolis on Biocompatibility of Cements: Morphological and Immunohistochemistry Analysis

2021 ◽  
Author(s):  
Izaura Helena Chaves de Meneses ◽  
Gêisa Aiane de Morais Sampaio ◽  
Rayssa Amaral Vieira ◽  
Márcio José da Silva Campos ◽  
Polliana Muniz Alves ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Inflammatory Infiltrate ◽  
Analysis Data ◽  
Giant Cells ◽  
Collagen Fibers ◽  
Control Groups ◽  
Time Intervals ◽  
Immunohistochemistry Analysis ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract Objective The focus of this study was to evaluate the biocompatibility of ionomer cements modified with ethanolic extracts of propolis (EEP) in different concentrations and time intervals. Materials and Methods In total, one hundred and thirty-five male Wistar rats were randomized into nine groups: Control, Groups Meron, and Groups Ketac (conventional, and added with 10, 25, 50% EEP, respectively). Histological analyses of inflammatory infiltrate and collagen fibers, and immunohistochemistry of CD68+ for macrophages (MOs) and multinucleated giant cells (MGCs) were performed. Statistical Analysis Data were analyzed using the Kruskal—Wallis and Dunn (p < 0.05) tests. Results Intense inflammatory infiltrate was demonstrated in the cements with 10% EEP at 7 days and 15 days (p < 0.05), only Group Ketac 10% EEP (p = 0.01) at 30 days. A smaller quantity of collagen fibers was observed in the cements with 10% EEP (p = 0.01) at 7 days, and Group Meron 10% EEP (p = 0.04) at 15 days. MOs and MGCs showed significant difference for the cements with 10% EEP (p = 0.01) at 7 and 15 days. At 30 days, MOs persisted in the Groups with 10% EEP. Conclusions The concentration of 10% EEP had the greatest influence on the inflammatory and tissue repair processes. The concentrations of 25 and 50% EEP demonstrated biocompatibility similar to that of cements that did not receive EEP.

scholarly journals Experimental Pulpitis Treated By Double-Pinhole Drainage

2019 ◽  
Vol 3 (2) ◽  
Keyword(s):  
Dental Pulp ◽  
Control Group ◽  
Pulp Chamber ◽  
Control Groups ◽  
Time Intervals ◽  
Irrigation Methods ◽  
Significant Difference ◽  
And Control ◽  
Experimental Group ◽  
Labial Surface

Objective: To search for a dental pulp preservative method in experimental pulpitis. Methods: 20 rabbits with experimental pulpitis were selected for this study; Teeth were randomly assigned to 2 groups (experimental and control groups) based on the final irrigation methods. In experimental group (double-pinhole drainage), the two pinholes were drilled in middle 1/3 dental cervix of buccal labial surface of tooth; the pinholes were inserted into infant transfusion needles then the two syringes were contacted with them, one for suck, another for irrigation. In control group, pinhole was sealed. Rabbits were executed in 1, 3, 5, 7 day. The inflammation in pulp chamber was assessed after 1, 3, 5, and 7 days. Results: At the 1, 5 day time intervals, control group reported more inflammation than experimental group (P < .05). There was no significant difference between two groups at the other time intervals (P > .05), and in all groups the inflammation decreased over time. Conclusion: Dental pulp can be preserved by double-pinhole drainage efficiently.

scholarly journals Effect of degree of conversion on in vivo biocompatibility of flowable resin used for bioprotection of mini-implants

2015 ◽  
Vol 86 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Rogério Lacerda-Santos ◽  
Izaura Helena Chaves de Meneses ◽  
Gêisa Aiane de Morais Sampaio ◽  
Matheus Melo Pithon ◽  
Polliana Muniz Alves
Keyword(s):  
Granulation Tissue ◽  
Inflammatory Infiltrate ◽  
Monomer Conversion ◽  
Giant Cells ◽  
Collagen Fibers ◽  
Degree Of Conversion ◽  
Repair Capacity ◽  
Collagen Formation ◽  
Mini Implants ◽  
Group Control

ABSTRACT Objective:  To test the hypothesis that there is no difference between the biocompatibility and degree of monomer conversion of flowable resins used as bioprotective materials of orthodontic mini-implants. Materials and Methods:  Forty-eight male Wistar rats were divided into four groups (n  =  12). Group Control (polyethylene), Group Wave, Group Top Comfort, and Group Filtek. The animals were sacrificed after time intervals of 7, 15, and 30 days and tissues were analyzed under optical microscopy for inflammatory infiltrate, edema, necrosis, granulation tissue, multinucleated giant cells, and collagen formation. The degree of conversion was evaluated by the Fourier method. Biocompatibility and degree of conversion were evaluated by the Kruskal-Wallis and Dunn tests, and analysis of variance and the Tukey test, respectively (P &lt; .05). Results:  An intense inflammatory infiltrate was observed on the seventh day, with Groups Top Comfort and Filtek differing statistically from Group Control (P  =  .016). Edema, necrosis, granulation tissue, and giant cells showed greater expressiveness at 7 days, without statistical difference between them (P &gt; .05). For the presence of collagen fibers, Group Top Comfort was shown to differ statistically from Group Control (P  =  .037) at 15 days and from Groups Filtek and Control (P  =  .008) at 30 days. Monomer conversion ranged from 62.3% in Group Top Comfort at 7 days to 79.1% in Group Filtek at 30 days. Conclusions:  The hypothesis was rejected. The resin Top Comfort demonstrated lower tissue repair capacity with a lower number of collagen fibers compared with Filtek and Wave resins. The resin Top Comfort showed the lowest conversion values during the experiment.

scholarly journals Impact of Buprenorphine on Learning and Memory Ability Related to the Acetylcholinesterase Change in the Hippocampus of Rats

2021 ◽  
Vol 11 (5) ◽  
pp. 13111-13114
Keyword(s):  
Memory Impairment ◽  
Ache Activity ◽  
Memory Function ◽  
Male Rats ◽  
Control Groups ◽  
Treatment Groups ◽  
Activity Inhibition ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Impact

Buprenorphine (BUP), a “synthetic opioid”, may cause memory impairment. This investigation aimed to study the impact of BUP on memory function related to acetylcholinesterase (AChE) activity inhibition in male rats. 24 male Wistar rats were randomly divided into three groups; control (C) and two treatment groups BUP (0.3 and 1) (n=8, for each group). BUP (0.3 and 1 mg/kg) was administrated subcutaneously once a day for 30 days. Normal saline 0.9% was injected in to control groups. In the end, animals were anesthetized and decapitated, and their hippocampus was dissected to assess AChE activity. There were no significant differences between the activities of AChE in the hippocampus in BUP-treated animals compared with controls. Besides, the activities of AChE in the BUP 0.3 group and BUP 1 group did not indicate a significant difference. These findings did not confirm the effect of BUP at doses of 0.3 and 1 mg/kg on memory function associated with the AChE activity inhibition.

scholarly journals The photobiomodulation (658, 830 and 904nm) on wound healing in histomorphometric analysis

2020 ◽  
Vol 33 ◽  
Author(s):  
Letícia Sandis Barbosa ◽  
Julia Risso Parisi ◽  
Lucas do Carmo Viana ◽  
Marcella Bernucci Carneiro ◽  
Josie Resende Torres da Silva ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Repair Process ◽  
Skin Lesions ◽  
Volume Density ◽  
Collagen Fibers ◽  
Control Group ◽  
Type Iii Collagen ◽  
Type Iii ◽  
Significant Difference ◽  
Injured Region

Abstract Introduction: Photobiomodulation (PBM) assists in the processes of angiogenesis and cellular mitosis after skin lesion, contributing to tissue repair. Objective: to investigate the effects of photobiomodulation (during the proliferative phase) of 658 nm, 830 nm and 904 nm in the repair of skin lesions in an animal model. Method: 658 nm (G658), 830 nm (G830), 904 nm (G904) PBM, and control group (CG) integrated the research. We submitted the animals to an excisional wound and treatment at different wavelengths for 14 days. On the seventh and 14-1485004059th postoperative days, we calculated the area and percentage of lesion contraction. The animals were sacrificed on the 14-1485004056th postoperative day and cutaneous section of the injured region was collected for histomorphometric evaluation of the cellularity, neovascularization, thickness of the epidermis and volume density of collagen fibers colored with H&E and Picross Sirius respectively. For the statistical analysis, we applied the ANOVA test. Results: the G658 presented higher cellularity than GC (p = 0.03). The animals in the G658 group showed a significant increase in the neovascularization in relation to the CG (p = 0.01). Type III collagen significantly increased in G904 compared to G830 (p < 0.0001) and CG (p < 0.0001). The G658 had a significant increase in type III collagen fibers compared to G830 (p < 0.0001) and GC (p < 0.0001). We found no significant difference in the thickness of the epidermis, wound area, and in the percentage wound of contraction between the analyzed groups. Conclusion: PBM was effective to stimulate the tissue repair process, with better results for the 658 nm wavelength.

scholarly journals ANÁLISE MORFOLÓGICA DAS VIAS AÉREAS DE RATOS EXPOSTOS À FUMAÇA DO CIGARRO, E AO ÁLCOOL E SUA RELAÇÃO COM O EXERCÍCIO FÍSICO

2020 ◽  
Vol 12 (2) ◽  
pp. 35-41
Author(s):  
Fabiola de Azevedo Mello ◽  
Carolina Capelasso de Oliveira ◽  
Gabriela Vidotto Cavalieri ◽  
Armando Ribeiro Florido Neto ◽  
Lucas Agostini ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Alcohol Intake ◽  
Inflammatory Infiltrate ◽  
Inflammatory Cells ◽  
Respective Group ◽  
Physical Exercises ◽  
Significant Difference ◽  
Male Wistar Rats

The aim of the study was to quantify and compare, morphologically, the mucus and inflammatory infiltrate in rats exposed to cigarette smoke, alcohol and both agents, including physical exercise. Forty male Wistar rats were used, randomly divided into four groups. Control animals performed physical exercises. The other animals were exposed to cigarette smoke, alcohol intake and physical training, according to the respective group. The lungs of the animals were collected to assess the findings. There was no significant difference between the groups regarding the assessment of the airway perimeter and the amount of inflammatory cells in the animals' airways. It was concluded that there was no influence of physical exercise on the production of mucus and inflammatory infiltrate in rats exposed to alcohol and cigarette smoke. Thus, it is suggested to carry out further studies in order to elucidate the results.

scholarly journals Biocompatibility, Biomineralization, and Maturation of Collagen by RTR®, Bioglass and DM Bone® Materials

2020 ◽  
Vol 31 (5) ◽  
pp. 477-484
Author(s):  
Francine Benetti ◽  
Carlos Roberto Emerenciano Bueno ◽  
Alexandre Henrique dos Reis-Prado ◽  
Marina Trevelin Souza ◽  
Juliana Goto ◽  
...  
Keyword(s):  
Collagen Fiber ◽  
Subcutaneous Tissue ◽  
Polarized Light ◽  
Collagen Fibers ◽  
Control Group ◽  
Tissue Replacement ◽  
Parametric Data ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract This study evaluated the biocompatibility, biomineralization, and collagen fiber maturation induced by Resorbable Tissue Replacement (RTR®; β-tricalcium phosphate [TCP]), Bioglass (BIOG; bioactive glass), and DM Bone® (DMB; hydroxyapatite and β-TCP) in vivo. Sixty-four polyethylene tubes with or without (control group; CG) materials (n=8/group/period) were randomly implanted in the subcutaneous tissue of 16 male Wistar rats (four per rat), weighting 250 to 280 g. The rats were killed after 7 and 30 days (n=8), and the specimens were removed for analysis of inflammation using hematoxylin-eosin; biomineralization assay using von Kossa (VK) staining and polarized light (PL); and collagen fiber maturation using picrosirius red (PSR). Nonparametric data were statistically analyzed by Kruskal-Wallis and Dunn tests, and parametric data by one-way ANOVA test (p<0.05). At 7 days, all groups induced moderate inflammation (p>0.05). At 30 days, there was mild inflammation in the BIOG and CG, and moderate inflammation in the RTR and DMB groups, with a significant difference between the CG and RTR (p<0.05). The fibrous capsule was thick at 7 days and predominantly thin at 30 days in all groups. All materials exhibited structures that stained positively for VK and PL. Immature collagen fibers were predominant at 7 and 30 days in all groups (p>0.05), although DMB exhibited more mature fibers than BIOG at 30 days (p<0.05). RTR, BIOG, and DMB were biocompatible, inducing inflammation that reduced over time and biomineralization in the subcutaneous tissue of rats. DMB exhibited more mature collagen fibers than BIOG over a longer period.

scholarly journals Valuation of angiogenesis in bovine xenografts implanted in intracorporal sites of rabbits as models of in-vivo bioreactors

2021 ◽  
Vol 73 (5) ◽  
pp. 1067-1075
Author(s):  
G.L. Montanhim ◽  
M.T.S. Silva ◽  
M.E.B.A.M. Conceição ◽  
T.S. Rocha ◽  
L.M. Yamashiro ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Bone Substitute ◽  
Inflammatory Infiltrate ◽  
Inflammatory Cells ◽  
Giant Cells ◽  
Vascular Endothelial ◽  
Evaluation Time ◽  
Significant Difference ◽  
Endothelial Growth Factor

ABSTRACT The aim of this study was to evaluate neovascularization of bovine xenografts implanted in intracorporeal sites of rabbits (bioreactors). 30 rabbits were used, divided into 6 groups, according to the evaluation time (7, 15, 30, 45, and 60 days); each animal received xenogenic implants in 3 different intracorporeal sites (A1 - omentum bag; A2 - intermuscular space of quadriceps femoris; A3 - subperiosteal of ilium bone). Histological assessments graded the presence of angiogenesis, the number of inflammatory cells, newly formed bone tissue, and the presence of giant cells. Histological analyses showed intense angiogenesis in all implanted xenografts. Presence of inflammatory infiltrate and giant cells at the A1 implant site and presence of bone neoformation at the A3 implant site were noted. Degeneration of implants and formation of a fibrous capsule were noted. When comparing the interaction of the site with the days of evaluation, statistical analysis showed a significant difference (p≤0.05) in any time of neovascularization analysis. The vascular endothelial growth factor (VEGF) and inflammatory cells of the omentum in its structure, may have contributed to the greater presence of neovessels and inflammatory cells, a fact that may indicate functionality as a possible bone substitute.

scholarly journals THE EFFECT OF RAMANIA LEAF (Bouea macrophylla Griff) EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN INCISIONAL WOUND OF MALE WISTAR RATS

2021 ◽  
Vol 6 (1) ◽  
pp. 96
Author(s):  
Dinda Rizkia ◽  
Irham Taufiqurrahman ◽  
Renie Kumala Dewi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Collagen Fibers ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Post Hoc

ABSTRACTBackground: Ramania leaf (Bouea macrophylla Griff) extract gel has secondary metabolites in the form of flavonoids, steroids, phenols and terpenoids which have a role as antioxidant. They will protect the body from excessive production of reactive oxygen species (ROS) by increasing endogenous antioxidants SOD, CAT and GPX, so that wound healing will not be inhibited and the process of collagen synthesis can run smoothly. Objective: To analyze the effect of ramania leaf extract gel that was applied topically with 5%, 10% and 15% concentration on collagen fibers density in incisional wound of male Wistar rats (Rattus norvegicus) on the 7th and the 14th day. Method: This research is a pure experimental study with a posttest-only control group design, using 24 rats which were divided into 4 groups: the treatment groups given ramania leaf extract gel of 5%, 10%, 15%, and the control group given placebo gel. The application of the extract gel was done once within 24 hours. The collagen level was measured with a spectrophotometer on the 7th and the 14th day. Results: Two-Way ANOVA test results on the 7th and the 14th day of each group showed a significant difference with p=0.000 (p<0.05). The Bonferroni Post-hoc Test showed a significant difference with p<0.05 between the placebo gel group and the groups of ramania leaf extract gel of 5%, 10%, 15% on the 7th and the 14th day. Conclusion: There is an effect of ramania leaf extract gel on collagen fibers density with the most effective concentration of 15%.Keywords: Antioxidant, , Collagen, Ramania Leaf Extract Gel

scholarly journals COULD THE INTESTINAL EPITHELIAL ALTERATIONS PROMOTED BY ROUX-EN-Y GASTRIC BYPASS EXPLAIN HIGHER TENDENCY FOR COLONIC DISEASES IN BARIATRIC PATIENTS?

2020 ◽  
Vol 33 (4) ◽  
Author(s):  
Eduardo WENDLER ◽  
Osvaldo MALAFAIA ◽  
Bruno Luiz ARIEDE ◽  
Jurandir Marcondes RIBAS-FILHO ◽  
Nicolau Gregori CZECZKO ◽  
...  
Keyword(s):  
Experimental Studies ◽  
Nuclear Staining ◽  
Intestinal Epithelial ◽  
Ascending Colon ◽  
Control Groups ◽  
Ki 67 ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Bariatric Patients ◽  
And Control

ABSTRACT Background: Intestinal diversions have revolutionized the treatment of morbid obesity due to its viability and sustained response. However, experimental studies suggest, after these derivations, a higher risk of colon cancer. Aim: To analyze the histological and immunohistological changes that the jejunojejunal shunt can produce in the jejunum, ileum and ascending colon. Method: Twenty-four male Wistar rats were randomly divided into two groups, control (n=12) and experiment (n=12) and subdivided into groups of four. Nine weeks after the jejunojejunal shunt, segmental resection of the excluded jejunum, terminal ileum and ascending colon was performed. Histological analysis focused on the thickness of the mucosa, height of the villi, depth of the crypts and immunohistochemistry in the expression of Ki-67 and p53. Results: Significant differences were found between the experiment and control groups in relation to the thickness of the mucosa in the jejunum (p=0.011), in the ileum (p<0.001) and in the colon (p=0.027). There was also a significant difference in relation to the height of the villus in the ileum (p<0.001) and the depth of the crypts in the jejunum (p0.001). The results indicated that there is a significant difference between the groups regarding the expression of Ki-67 in the colon (p<0.001). No significant differences were found between the groups regarding the expression of Ki-67 in the jejunum and ileum. In the P53 evaluation, negative nuclear staining was found in all cases. Conclusion: The jejunojejunal deviation performed in the Roux-in-Y gastrojejunal bypass, predispose epithelial proliferative effects, causing an increase in the thickness of the mucosa, height of the villi and depth of the crypts of the jejunum, ileum and ascending colon.

scholarly journals The biocompatibility of a new endodontic paste used in dental trauma

2015 ◽  
Vol 44 (4) ◽  
pp. 232-238
Author(s):  
Adriana de Jesus Soares ◽  
Maíra do Prado ◽  
Mariana Amade Brazão ◽  
Brenda Paula Figueiredo de Almeida Gomes ◽  
Alexandre Augusto Zaia
Keyword(s):  
Tissue Repair ◽  
Subcutaneous Tissue ◽  
Dental Trauma ◽  
Tissue Reaction ◽  
Short Term ◽  
Time Intervals ◽  
Male Wistar Rats ◽  
Histological Processing ◽  
Materials Used ◽  
The Individual

<title>Abstract</title><sec><title>Objective</title><p>This study investigated the short-term subcutaneous tissue reaction of a new endodontic paste, based on the association among calcium hydroxide [Ca(OH)<sub>2</sub>], 2% chlorhexidine gel (CHX) and zinc oxide (ZnO) in comparison with a control paste, and the individual substances alone.</p></sec><sec><title>Material and method</title><p>Twenty-four male wistar rats were used. Polyethylene tubes containing the materials were implanted into the dorsal connective tissue of rats. Empty implanted tubes served as controls. The materials used were: Ca(OH)<sub>2</sub>+CHX+ZnO paste in two different proportions (2:1:2) and (2:1:3), a control paste, Ca(OH)<sub>2</sub>+CHX (2:1), and the substances alone. After time intervals of 2, 3, 7 and 15 days, the animals were euthanized and the specimens prepared for histological processing. Data were evaluated qualitatively according to the levels of tissue repair and the degree of inflammation.</p></sec><sec><title>Result</title><p>On the second day all the materials were observed to cause a severe inflammatory response. On the third day, the pastes Ca(OH)<sub>2</sub>+CHX+ZnO (2:1:2) and (2:1:3) showed reduction in degree of inflammation. On seventh day the tissue was already more organized with presence of collagen fibers in all materials. After 15 days it was found that compound Ca(OH)<sub>2</sub>+CHX+ZnO (2:1:2) showed areas with no inflammatory infiltrate.</p></sec><sec><title>Conclusion</title><p>The experimental pastes, Ca(OH)<sub>2</sub>+CHX+ZnO in two different proportions (2:1:2) and (2:1:3), showed favorable results with respect to biocompatibility, when evaluated for a short-term.</p></sec>

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