Defining the waterlogging tolerance of Ornithopus spp. for the temperate pasture zone of southern Australia

2020 ◽  
Vol 71 (5) ◽  
pp. 506
Author(s):  
D. R. Kidd ◽  
C. E. Di Bella ◽  
L. Kotula ◽  
T. D. Colmer ◽  
M. H. Ryan ◽  
...  

Increasing the area sown to Ornithopus spp. (serradella) can reduce overall fertiliser requirements in Australian permanent pastures owing to their greater nutrient-acquisition efficiency than that of more widely used pasture legumes such as Trifolium spp. However, uncertainty regarding waterlogging tolerance of Ornithopus spp. may restrict their adoption in the high-rainfall zone of southern Australia. The waterlogging tolerance of cultivars and accessions of three species of Ornithopus (O. compressus, O. sativus and O. pinnatus) was determined by comparing root and shoot growth of plants in deoxygenated, stagnant agar nutrient solution (simulated waterlogging) with growth in aerated nutrient solution. The responses were benchmarked against the known waterlogging-tolerant pasture legume Trifolium michelianum. All Ornithopus cultivars were highly impacted by the deoxygenated stagnant treatment, including those of the anecdotally waterlogging-tolerant O. pinnatus. The 14-day stagnant treatment reduced root dry mass by 32–62% and relative growth rate (RGR) of roots by 36–73%. At the same time, root porosity increased from 1.4% to 8.8%. Following a 14-day recovery period, during which plants were returned to aerated nutrient solution, Ornithopus spp. failed to increase their shoot RGR (particularly for O. sativus cultivars); however, root RGR returned to that of the aerated controls. The stagnant conditions inhibited transport of potassium (K+) to the shoots in all species, as evidenced by lower shoot tissue K+ concentrations, with O. compressus and O. sativus most adversely affected (45% and 48% of the tissue concentration of aerated control plants). We conclude that the suggested area for Ornithopus spp. adaptation should not preclude areas of high rainfall because they have root adaptations that would assist them in coping with transient water excess; however, soil types and surface profiles conducive to long-term waterlogging should be avoided to negate significant productivity losses.


2021 ◽  
Vol 22 (15) ◽  
pp. 8197
Author(s):  
Kinga Kęska ◽  
Michał Wojciech Szcześniak ◽  
Adela Adamus ◽  
Małgorzata Czernicka

Low oxygen level is a phenomenon often occurring during the cucumber cultivation period. Genes involved in adaptations to stress can be regulated by non-coding RNA. The aim was the identification of long non-coding RNAs (lncRNAs) involved in the response to long-term waterlogging stress in two cucumber haploid lines, i.e., DH2 (waterlogging tolerant—WL-T) and DH4 (waterlogging sensitive—WL-S). Plants, at the juvenile stage, were waterlogged for 7 days (non-primed, 1xH), and after a 14-day recovery period, plants were stressed again for another 7 days (primed, 2xH). Roots were collected for high-throughput RNA sequencing. Implementation of the bioinformatic pipeline made it possible to determine specific lncRNAs for non-primed and primed plants of both accessions, highlighting differential responses to hypoxia stress. In total, 3738 lncRNA molecules were identified. The highest number (1476) of unique lncRNAs was determined for non-primed WL-S plants. Seventy-one lncRNAs were depicted as potentially being involved in acquiring tolerance to hypoxia in cucumber. Understanding the mechanism of gene regulation under long-term waterlogging by lncRNAs and their interactions with miRNAs provides sufficient information in terms of adaptation to the oxygen deprivation in cucumber. To the best of our knowledge, this is the first report concerning the role of lncRNAs in the regulation of long-term waterlogging tolerance by priming application in cucumber.



HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1161f-1161
Author(s):  
John D. Lea-Cox ◽  
Irwin E. Smith

Pine bark is utilized as a substrate in citrus nurseries in South Africa. The Nitrogen (N) content of pine bark is inherently low, and due to the volubility of N, must be supplied on a continual basis to ensure optimum growth rates of young citrus nursery stock. Three citrus rootstock (rough lemon, carrizo citrange and cleopatra mandarin) showed no difference in stem diameter or total dry mass (TDM) when supplied N at concentrations between 25 and 200 mg ·l-1 N in the nutrient solution over a 12 month growing period. Free leaf arginine increased when N was supplied at 400 mg·l-1 N. The form of N affected the growth of rough lemon. High NH4-N:NO3-N (75:25) ratios decreased TDM when Sulfur (S) was absent from the nutrient solution, but not if S was present. Free arginine increased in leaves at high NH4-N (No S) ratios, but not at high NH4-N (S supplied) ratios. Free leaf arginine was correlated with free leaf ammonia. These results have important implications for reducing the concentration of N in nutrient solutions used in citrus nurseries and may indicate that higher NH4-N ratios can be used when adequate S is also supplied.



2021 ◽  
pp. 026540752110095
Author(s):  
Sandra Gloor ◽  
Stefanie Gonin-Spahni ◽  
Hansjörg Znoj ◽  
Pasqualina Perrig-Chiello

Separation and divorce are life events that might alter life satisfaction on the long term. Previous literature suggests a recovery of life satisfaction after marital dissolution for most individuals, simultaneously emphasizing considerable heterogeneity in the extent and timing of such adaptation. A new partnership seems to be a promising factor for a positive post-separation adjustment process. At the same time, the question arises whether people with beneficial characteristics, such as higher trait resilience, may have a double advantage by recovering faster and being more likely to find a new partner. However, little research has concurrently investigated the consequences of repartnering and favorable personal attributes on life satisfaction of middle- and older-aged people, especially not beyond the initial recovery period. Our data stem from a three-wave longitudinal questionnaire study including 199 participants after a separation, all single at the beginning of the study. Latent growth modeling was applied to investigate trajectories of life satisfaction, examining the role of a new intimate partnership, while controlling for trait resilience. Trait resilience represented a strong general predictor for inter-individual differences in the initial level of life satisfaction. However, an increase in life satisfaction over time was only observed in the group of participants who engaged in a new partnership. This increase occurred independently of preexisting differences in beneficial characteristics, such as high levels of trait resilience or life satisfaction. Thus, repartnering is associated with improvement of life satisfaction beyond the first adaptation phase after separation and is therefore important to prevent long-term negative consequences.



Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 189
Author(s):  
Kinga Kęska ◽  
Michał Wojciech Szcześniak ◽  
Izabela Makałowska ◽  
Małgorzata Czernicka

Waterlogging (WL), excess water in the soil, is a phenomenon often occurring during plant cultivation causing low oxygen levels (hypoxia) in the soil. The aim of this study was to identify candidate genes involved in long-term waterlogging tolerance in cucumber using RNA sequencing. Here, we also determined how waterlogging pre-treatment (priming) influenced long-term memory in WL tolerant (WL-T) and WL sensitive (WL-S) i.e., DH2 and DH4 accessions, respectively. This work uncovered various differentially expressed genes (DEGs) activated in the long-term recovery in both accessions. De novo assembly generated 36,712 transcripts with an average length of 2236 bp. The results revealed that long-term waterlogging had divergent impacts on gene expression in WL-T DH2 and WL-S DH4 cucumber accessions: after 7 days of waterlogging, more DEGs in comparison to control conditions were identified in WL-S DH4 (8927) than in WL-T DH2 (5957). Additionally, 11,619 and 5007 DEGs were identified after a second waterlogging treatment in the WL-S and WL-T accessions, respectively. We identified genes associated with WL in cucumber that were especially related to enhanced glycolysis, adventitious roots development, and amino acid metabolism. qRT-PCR assay for hypoxia marker genes i.e., alcohol dehydrogenase (adh), 1-aminocyclopropane-1-carboxylate oxidase (aco) and long chain acyl-CoA synthetase 6 (lacs6) confirmed differences in response to waterlogging stress between sensitive and tolerant cucumbers and effectiveness of priming to enhance stress tolerance.



2011 ◽  
Vol 1 (2) ◽  
pp. 59-63
Author(s):  
Tabish Hussain ◽  
Li Yu Shu ◽  
A Seid Adji ◽  
Tumenjavkhlan Sosorburam

Objective: Cardiac failure is a global burden among cardiovascular diseases, and major cause of morbidity and mortality especially among elderly age group. Angiotensin Converting Enzyme(ACE) inhibitors, remained the choice of treatment as they inhibit the renin angiotensin aldosterone system along with reduction in levels of pro-inflammatory cytokines, both of them are key factors in progression and complications of heart failure. The aim of this study was to rule out the effect of aging and efficacy of ACE inhibitor, Captopril, among Chinese cardiovascular patients with acute myocardial infarction (MI) during the hospitalization phase of therapy. Material & Methods: Randomized control trial at hospital of Tongji Medical College Wuhan, China over a period of more than 1 year from April 2009 till July 2010 recruiting patients in two stages. A total of 260 patients with mean age 65±8 years were recruited. All suffered from first time myocardial infarction and arrived in Cardiac emergency within 72 hours of the event. The participants were then randomly divided in study and control groups which were then further classified in sub-groups depending upon their age. Study group received ACE inhibitor Captopril in addition to standardized therapy while control group just received the conventional therapy for the event. Statistical analyses were done to formulate the corre-lation between multi-variables. Results: Participants were divided in Study group (N=150) (A and C, Young and Old) and the control group (N=110) (B and D, Young and Old). Survival rate was better among elderly on captopril in comparison to younger ones during the hospitalization. The Systolic blood pressure among study group was significantly lower than control group (132.9±16.3mmHg/84.7 ±9.1mmHg vs. 147.1±17.4mmHg/85.1 ±10.9mmHg, P<0.05).Patient’s survival was statistically significant with respect to age (P<0.001). Conclusion: Treatment with Captopril is definitely associated with improved short as well as long term cardiac prognosis and markedly. Captopril therapy is associated with improved long term prognosis and reduced cardiac mortality during the hospitalization phase of the therapy and recovery period. But the most significant finding is that the increased survival after taking Captopril was higher in elderly patients than in younger patients. ACE inhibitors like Captopril in proper dosage play a real vital beneficial role among elderly patients as compared to the younger ones, but still there is need to recruit a large cohort in different ethnic groups with different genetic makeup. Key Words: Myocardial infarction (MI); Ace-inhibitor; Aging DOI: 10.3126/ajms.v1i2.2947Asian Journal of Medical Sciences 1 (2010) 59-63





Author(s):  
Ziedonis Miklašēvičs

The methodology in Latvia forest industry provide to determine the quality of energy chips only in long- term storage places before selling. Due to the lack of hard empirical data about the quality parameters of energy chips in different phases of manufacturing process, this research paper consists of: - the identification and analyses of the factors that influenced the values of energy chips quality features such as: bulk density, moisture content, ash content, higher and lower heating value according to actual moisture content and per dry mass of the chips; - the methodology for determination the quality parameters of energy chips by analysis the wood moisture content and by choice the method of the manufacturing of energy chips.



HortScience ◽  
2021 ◽  
pp. 1-9
Author(s):  
Patience Seyram Akakpo ◽  
Moosa Mahmood Sedibe ◽  
Bello Zaid ◽  
Zenzile P. Khetsha ◽  
Mokgaputsiwa P. Theka-Kutumela ◽  
...  

Potassium (K) is an essential nutrient in plant metabolism, ionic balance, and stress resistance. In this study, the effects of K on agronomic attributes and on mineral and primary metabolite content in African potato were determined. K was administered hydroponically at four concentrations (4.00, 6.00, 8.00, and 10.00 meq·L−1) using Steiner’s universal nutrient solution. Chlorophyll content (CHL), leaf area (LA), fresh corm mass (FCM), number of roots (NR), root fresh weight (RFM), and root dry mass (RDM) were measured 18, 32, and 40 weeks after transplanting. Mineral analysis data were collected at 18 weeks, and primary metabolite data were collected at 32 weeks. Significant effects of K were observed after 18 weeks, and all test concentrations had a positive effect on yield. Calcium and boron significantly accumulated in the corm at 4.00 meq·L−1 K. Alanine and malic acid were the only metabolites affected by K concentrations. More minerals accumulated in the corm at 4.00 meq·L−1 K, whereas at 10.00 meq·L−1 K, more minerals clustered in the leaf. K applied at 4.00 meq·L−1 is recommended when growing African potato using a nutrient solution to improve corm mineral and metabolite accumulation.



2001 ◽  
Vol 52 (3) ◽  
pp. 329 ◽  
Author(s):  
G. D. Li ◽  
K. R. Helyar ◽  
M. K. Conyers ◽  
B. R. Cullis ◽  
P. D. Cregan ◽  
...  

A long-term trial, known as ‘managing acid soils through efficient rotations’ (MASTER), commenced in 1992 to develop and demonstrate a cropping system that is economically viable on the highly acid soils of the traditional permanent pasture region in south-eastern Australia, so that their fertility is sustained or improved. There were 2 permanent pasture systems and 2 pasture–crop rotations, each with and without lime. This paper reports the effect of lime on crop production over the first cycle (6 years). On annual pasture–crop rotations, lime significantly increased the dry matter production at anthesis and grain yields of wheat (cv. Dollarbird) compared with the unlimed treatments. Averaged across years from 1992 to 1997 (excluding the severe drought year 1994), wheat crops produced 1.6 t/ha more grain on the limed treatments than on the unlimed treatments (3.6 v. 2.0 t/ha). On perennial pasture–crop rotations, the lime effects varied with crops grown at each phase and year. For example, despite being tolerant of acidity, oats (cv. Yarran) responded to lime in 1996. Likewise, triticale (cv. Abacus) responded to lime in 1997. Wheat (cv. Dollarbird) that is moderately tolerant to acidity responded to lime in phase 6 from 1992 to 1997 excluding 1994 (3.5 v. 1.7 t/ha). Acid-tolerant wheat varieties, triticale, and narrow-leaf lupins are considered the most viable crops for the soil and climatic conditions encountered in this high rainfall (5000—800 mm per annum) area of south-eastern Australia.



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