Chilling at 0 deg C in air induces ethylene production in Fuji and Lady Williams apples

1995 ◽  
Vol 35 (5) ◽  
pp. 651 ◽  
Author(s):  
J Jobling ◽  
WB McGlasson

Fuji apples have low rates of ethylene production at commercial harvest maturity, whereas Lady Williams apples, which are harvested commercially in late autumn, produce large concentrations of ethylene. We investigated whether chilling at 0�C, which has previously been shown to stimulate ethylene synthesis in preclimacteric Granny Smith apples, would stimulate Fuji and Lady Williams fruit as well. Preclimacteric Fuji and Lady Williams apples were stored in air at 0�C and were removed at intervals to 20�C. Ethylene production remained low in Fuji apples held continuously in air at 20�C. Fruit held for 32 days at 0�C accumulated 1-aminocyclopropane-1-carboxylic acid (ACC) and developed ACC oxidase activity. On return to 20�C these fruit had a high and sustained level of ethylene production. ACC accumulated in all chilled Lady Williams apples at 0�C but decreased to low levels within 2 days after transfer to 20�C. A sustained increase in ACC was not found until 15 days at 20�C in fruit chilled for 32 days. A distinct temporary increase in internal carbon dioxide (CO2) concentration was observed in Fuji apples but not in Lady Williams apples following chilling. Chilling for 32 days was required to stimulate sustained levels of CO2 in Lady Williams apples.

1999 ◽  
Vol 5 (3) ◽  
pp. 223-228 ◽  
Author(s):  
C. Larrigaudiere ◽  
I. Recasens ◽  
J. Graell ◽  
M. Vendrell

Changes in 1-aminocyclopropane-1-carboxylic acid metabolism in apples ( Malus domestica Borkh cv Granny Smith) were studied in relation to cold storage. Emphasis was given to the differential re sponsiveness of fruits to cold treatment as a function of stage of maturity at harvest. Fruits were held at 1 or 20 °C for 30 days, respectively, or exposed to 1 °C for 10 days and then storaged at 20 °C for up to 30 days. Fruits at 20 °C showed typical climacteric behavior. Differences at 1 °C between maturity stages in ethylene production and ACC oxidase activity were abolished, which showed that cold treatment is an important inducer of climacteric rise in preclimacteric Granny Smith apples. At 1 °C, ethylene production was lower than at 20 °C and the maxima in production were similar for all the stages of maturity, but took place at different times which corresponded exactly to the initial differ ences in harvest dates. After the transfer to 20 °C, fruits exhibited similar behavior as regards ethyl ene production, ACC oxidase activity, and ACC and MACC levels in relation to a harmonization process which is discussed in this study.


HortScience ◽  
2000 ◽  
Vol 35 (7) ◽  
pp. 1294-1299 ◽  
Author(s):  
M. Clayton ◽  
W.V. Biasi ◽  
S.M. Southwick ◽  
E.J. Mitcham

ReTain™, a commercial derivative of aminoethoxyvinylglycine, was applied as a single application at 124 g·ha-1 a.i. to `Bartlett' pear (Pyrus communis L.) trees 28, 21, 14, or 7 days prior to initial commercial harvest and at 62 g·ha-1 a.i. in combination with naphthaleneacetic acid (NAA) at 92 g·ha-1 a.i. 14 days prior to initial commercial harvest. Maturity and quality of treated fruits at harvest and following storage were compared with those of nontreated pears in 1996 and 1997. Ethylene production by mature green pears at harvest was not significantly affected by ReTain™ treatments, although softening, loss of chlorophyll, and starch clearance were usually inhibited by the 14- or 7-day treatment. ReTain™ suppressed ethylene production, softening and loss of chlorophyll in ripening pears and mature green pears cold-stored for 4 months, although loss of chlorophyll did not differ in the cold-stored fruit in 1997. ReTain™ had little effect on softening during a ripening period of 6 days after 4 months of cold storage. Application at 14 or 7 days prior to initial harvest appeared most effective, often with little difference between the two timings, and the 28- or 21-day treatment or combined ReTain™ and NAA treatment were seldom more effective. ReTain™ applied 14 or 7 days before initial harvest delayed fruit maturation by 4-10 days depending on the maturity index. The maturity or ripeness of pears from the combined ReTain™ and NAA, NAA only, and control treatments was often similar or differed only slightly. Premature ripening, prevalent in 1997, was dramatically suppressed in fruit treated with ReTain™. Ripening of both ReTain™- and non-ReTain™-treated fruit with ethylene reduced premature ripening by ≈50%.


HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 1074-1078 ◽  
Author(s):  
Pankaj Kumar Bhowmik ◽  
Toshiyuki Matsui

The molecular and enzymatic changes that follow harvesting of asparagus are important aspects for postharvest deterioration. To define the factors contributing to postharvest deterioration, the early changes in ethylene production and the activities and expression of 1-aminocyclopropane-1-carboxylate (ACC) synthase, ACC oxidase, and phenylalanine ammonia-lyase (PAL) were studied in whole spears and in excised top and bottom portions. As a result of wounding, ethylene production was found higher in both top and bottom portions compared with whole spears but followed the same trend reaching the peak at 16 hours after harvest. ACC synthase was rapidly induced in excised top portions but no significant ACC synthase activity was observed in excised bottom portions. In top portions, ACC synthase reached a peak 8 hours after harvest and thereafter started to decline. In contrast to ACC synthase, ACC oxidase was induced markedly in both top and bottom portions and remained high until 16 hours after excision. On the other hand, PAL greatly increased in activity in bottom portions but not in excised tops. Northern blot analysis showed that increased mRNA levels coincided well with the excision-promoted increases in enzyme activity and ethylene production.


2013 ◽  
Vol 781-784 ◽  
pp. 1712-1716
Author(s):  
Li Ya Liang ◽  
Qiao Wang ◽  
Ling Li ◽  
Shi Jie Yan

The effects of 1-MCP treatment on the ethylene synthesis of Yali pear seeds of different harvest maturity were evaluated during storage. Yali pear fruit (early harvested and late harvested) were treated with 1.0 μL.L-11-MCP for 20 h at room temperature, packaged in PE plastic film and then stored at 0±1°C for 180 days. The results show that the respiration, ethylene production, ACC content, ACO and ACS activities of the seeds of early harvested Yali pear fruit with 1-MCP treatment decrease significantly during storage, the peaks decrease and are delayed for 60 days. But 1-MCP treatment has no significant effects on the indexes related with ethylene synthesis to the seeds of late harvested fruit. 1-MCP can be used to inhibit the synthesis of ethylene in the seeds effectively and keep the quality and extend the shelf life of early harvested Yali pear fruit.


Author(s):  
Jindong Wu ◽  
Jiantao Weng ◽  
Bing Xia ◽  
Yujie Zhao ◽  
Qiuji Song

High indoor air quality is crucial for the health of human beings. The purpose of this work is to analyze the synergistic effect of particulate matter 2.5 (PM2.5) and carbon dioxide (CO2) concentration on occupant satisfaction and work productivity. This study carried out a real-scale experiments in a meeting room with exposures of up to one hour. Indoor environment parameters, including air temperature, relative humidity, illuminance, and noise level, were controlled at a reasonable level. Twenty-nine young participants were participated in the experiments. Four mental tasks were conducted to quantitatively evaluate the work productivity of occupants and a questionnaire was used to access participants’ satisfaction. The Spearman correlation analysis and two-way analysis of variance were applied. It was found that the overall performance declined by 1% for every 10 μg/m3 increase in PM2.5 concentration. Moreover, for every 10% increase in dissatisfaction with air quality, productivity performance decreased by 1.1% or more. It should be noted that a high CO2 concentration (800 ppm) has a stronger negative effect on occupant satisfaction towards air quality than PM2.5 concentration in a non-ventilated room. In order to obtain optimal occupant satisfaction and work productivity, low concentrations of PM2.5 (<50 μg/m3) and CO2 (<700 ppm) are recommended.


Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 71
Author(s):  
María Carmen Antolín ◽  
María Toledo ◽  
Inmaculada Pascual ◽  
Juan José Irigoyen ◽  
Nieves Goicoechea

(1) Background: The associated increase in global mean surface temperature together with raised atmospheric carbon dioxide (CO2) concentration is exerting a profound influence on grapevine development (phenology) and grape quality. The exploitation of the local genetic diversity based on the recovery of ancient varieties has been proposed as an interesting option to cope with climate change and maintaining grape quality. Therefore, this research aimed to characterize the potential fruit quality of genotypes from seven local old grapevine varieties grown under climate change conditions. (2) Methods: The study was carried out on fruit-bearing cuttings (one cluster per plant) that were grown in pots in temperature gradient greenhouses (TGG). Two treatments were applied from fruit set to maturity: (1) ambient CO2 (400 ppm) and temperature (T) (ACAT) and (2) elevated CO2 (700 ppm) and temperature (T + 4 °C) (ECET). (3) Results: Results showed that some of the old genotypes tested remained quite stable during the climate change conditions in terms of fruit quality (mainly, total soluble solids and phenolic content) and of must antioxidant properties. (4) Conclusion: This research underlines the usefulness of exploiting local grapevine diversity to cope with climate change successfully, although further studies under field conditions and with whole plants are needed before extrapolating the results to the vineyard.


Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 995
Author(s):  
Mohammad Darvish ◽  
Habib Shirzad ◽  
Mohammadreza Asghari ◽  
Parviz Noruzi ◽  
Abolfazl Alirezalu ◽  
...  

Ethylene is the most important factor playing roles in senescence and deterioration of harvested crops including cut flowers. Brassinosteroids (BRs), as natural phytohormones, have been reported to differently modulate ethylene production and related senescence processes in different crops. This study was carried out to determine the effects of different levels of 24-epibrassinolide (EBL) on ACC oxidase enzyme activity, the final enzyme in ethylene biosynthesis pathway, vase life, and senescence rate in lisianthus cut flowers. Harvested flowers were treated with EBL (at 0, 3, 6, and 9 µmol/L) and kept at 25 °C for 15 days. The ACC oxidase activity, water absorption, malondialdehyde (MDA) production and vase solution absorption rates, chlorophyll and anthocyanin contents, and the vase life of the flowers were evaluated during and at the end of storage. EBL at 3 µmol/L significantly (p ≤ 0.01) enhanced the flower vase life by decreasing the ACC oxidase activity, MDA production and senescence rates, and enhancing chlorophyll and anthocyanin biosynthesis and accumulation, relative water content, and vase solution absorption rates. By increasing the concentration, EBL negatively affected the flower vase life and postharvest quality probably via enhancing the ACC oxidase enzyme activity and subsequent ethylene production. EBL at 6 and 9 µmol/L and in a concentration dependent manner, enhanced the ACC oxidase activity and MDA production rate and decreased chlorophyll and anthocyanin accumulation and water absorption rate. The results indicate that the effects of brassinosteroids on ethylene production and physiology of lisianthus cut flowers is highly dose dependent.


2008 ◽  
Vol 133 (6) ◽  
pp. 727-734 ◽  
Author(s):  
Hong Zhu ◽  
Eric P. Beers ◽  
Rongcai Yuan

Effects of naphthaleneacetic acid (NAA) and aminoethoxyvinylglycine (AVG) on young fruit abscission, leaf and fruit ethylene production, and expression of genes related to ethylene biosynthesis and cell wall degradation were examined in ‘Delicious’ apples (Malus ×domestica Borkh.). NAA at 15 mg·L−1 increased fruit abscission and ethylene production of leaves and fruit when applied at the 11-mm stage of fruit development, whereas AVG, an inhibitor of ethylene biosynthesis, at 250 mg·L−1 reduced NAA-induced fruit abscission and ethylene production of leaves and fruit. NAA also increased expression of 1-aminocyclopropane-1-carboxylate (ACC) synthase genes (MdACS5A and MdACS5B), ACC oxidase gene (MdACO1), and ethylene receptor genes (MdETR1a, MdETR1b, MdETR2, MdERS1, and MdERS2) in fruit cortex and fruit abscission zones. However, AVG reduced NAA-induced expression of these genes except for MdERS2 in fruit abscission zones. NAA increased expression of the polygalacturonase gene MdPG2 in fruit abscission zones but not in fruit cortex, whereas AVG reduced NAA-enhanced expression of MdPG2 in fruit abscission zones. The expression of β-1,4-glucanase gene MdCel1 in fruit abscission zones was decreased by NAA but was unaffected by AVG. Our results suggest that ethylene biosynthesis, ethylene perception, and the MdPG2 gene are involved in young fruit abscission caused by NAA.


2021 ◽  
Vol 11 (19) ◽  
pp. 9265
Author(s):  
Yingzi Zhang ◽  
Yanze Wang ◽  
Mingqian Yang ◽  
Huatao Wang ◽  
Guofang Chen ◽  
...  

Climate change has been unprecedented in the past decades or even thousands of years, which has had an adverse impact on the mechanical properties of concrete structures. Many researchers have begun to study new concrete materials. Graphene nanoplatelet (GNP) is an attractive nanomaterial that can change the crystal structure of concrete and improve durability. The aim of the present study was to investigate the effect of GNP (0.05%wt) on the carbonation depth of concrete under simulated changing climate conditions (varying temperature, relative humidity, and carbon dioxide (CO2) concentration), and compare it with ordinary concrete. When the concentration of CO2 is variable, the carbonation depth of graphene concrete is 10% to 20% lower than that of ordinary concrete. When the temperature is lower than 33 °C, the carbonation depth of graphene concrete is less than that of the control sample; however, above 33 °C, the thermal conductivity of GNP increases the carbonation reaction rate of concrete. When the humidity is a variable, the carbonation depth of graphene concrete is less than 15% to 30% of ordinary concrete, and when the humidity is higher than 78%, the difference in the carbonation depth between the ordinary concrete and the graphene concrete decreases gradually. The overall results indicated that GNP has a favorable effect on anti-carbonation performance under changing climate conditions.


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