scholarly journals DNA barcoding identifies cryptic animal tool materials

2021 ◽  
Vol 118 (29) ◽  
pp. e2020699118
Author(s):  
Matthew P. Steele ◽  
Linda E. Neaves ◽  
Barbara C. Klump ◽  
James J. H. St Clair ◽  
Joana R. S. M. Fernandes ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Raw Materials ◽  
Camera Traps ◽  
Behavioral Experiments ◽  
Plant Materials ◽  
Long Term Study ◽  
Fitness Consequences ◽  
Wide Range ◽  
Significant Research ◽  
New Caledonian Crows

Some animals fashion tools or constructions out of plant materials to aid foraging, reproduction, self-maintenance, or protection. Their choice of raw materials can affect the structure and properties of the resulting artifacts, with considerable fitness consequences. Documenting animals’ material preferences is challenging, however, as manufacture behavior is often difficult to observe directly, and materials may be processed so heavily that they lack identifying features. Here, we use DNA barcoding to identify, from just a few recovered tool specimens, the plant species New Caledonian crows (Corvus moneduloides) use for crafting elaborate hooked stick tools in one of our long-term study populations. The method succeeded where extensive fieldwork using an array of conventional approaches—including targeted observations, camera traps, radio-tracking, bird-mounted video cameras, and behavioral experiments with wild and temporarily captive subjects—had failed. We believe that DNA barcoding will prove useful for investigating many other tool and construction behaviors, helping to unlock significant research potential across a wide range of study systems.

scholarly journals Single-Laboratory Validation of a Two-Tiered DNA Barcoding Method for Raw Botanical Identification

2019 ◽  
Vol 102 (5) ◽  
pp. 1435-1447 ◽  
Author(s):  
Zhengfei Lu ◽  
Christopher M Thompson ◽  
Tiffany Chua ◽  
Silva Babajanian ◽  
Yanjun Zhang ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Raw Materials ◽  
Dna Barcode ◽  
Sampling Technique ◽  
Regulatory Compliance ◽  
Raw Material ◽  
Animal Products ◽  
Policy Makers ◽  
Plant Parts ◽  
Wide Range

Abstract Background: The applications of deoxyribonucleic acid (DNA) barcoding methods have been extended from authenticating taxonomic provenance of animal products to identifying botanicals used as herbal medicine and in botanical dietary supplements. DNA barcoding methods for botanical identification must be adequately validated to meet regulatory compliance. Objective: The goal of this study is to provide a validation protocol for a two-tiered DNA barcoding method that aims to identify raw botanicals. Methods: A barcode database was computationally validated to define the barcode combinations that can unambiguously identify botanicals in the database. A maximum variation sampling technique was used to capture a wide range of perspectives relating to DNA barcode-based botanical identification, including plant parts and species distance, for the experimental validation. Twenty-two authenticated botanicals were purposively sampled from different plant parts—covering both closely related and distantly related species—to validate the two-tiered DNA barcoding method. The performance of the method was assessed on accuracy, precision, ruggedness, and uncertainty. Results: High accuracy (100%) and precision (1.0) were obtained from the validation samples. The method was also found to be rugged and have acceptable uncertainty. Conclusions: The method was validated and suitable for DNA-based identification of botanical raw materials listed in the current database. Highlights: This work will provide support guidance for manufacturers and regulatory policy makers to implement equivalent validated and compliant DNA-based testing in quality control processes to improve botanical raw material identification and authentication.

scholarly journals Development of a DNA Barcoding-Like Approach to Detect Mustard Allergens in Wheat Flours

Genes ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 234 ◽  
Author(s):  
Jessica Frigerio ◽  
Roberta Pellesi ◽  
Valerio Mezzasalma ◽  
Fabrizio De Mattia ◽  
Andrea Galimberti ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Dna Barcoding ◽  
Raw Materials ◽  
Cross Reactivity ◽  
Food Allergens ◽  
Specific Primers ◽  
Wide Range ◽  
International Regulations ◽  
Set Up ◽  
Wheat Flours

The spread of food allergens is a topic of global importance due to its impact on public health. National and International regulations ask food producers and manufacturers to declare product compositions on the label, especially in case of processed raw materials. Wheat flour (Triticum aestivum) can be contaminated by a wide range of species belonging to the Brassicaceae in the field or during grain harvests, storage, and processing. Among them, mustards (Brassica nigra, Brassica juncea and Sinapis alba) are well known allergenic species. Often, food quality laboratories adopt an ELISA approach to detect the presence of mustard species. However, this approach shows cross-reactivity with other non-allergenic species such as Brassica napus (rapeseed). In the last few years, DNA barcoding was proposed as a valid identification method, and it is now commonly used in the authentication of food products. This study aims to set up an easy and rapid DNA-based tool to detect mustard allergenic species. DNA barcoding (matK and ITS2) and chromosome markers (A6, B, C1 genome regions) were selected, and specific primers were validated on incurred reference food matrices. The developed test was proven to be able to distinguish mustard from rapeseed and wheat, overcoming cross-reactivity with Brassica napus.

scholarly journals The use of bio-efficient plant materials to optimize the fatty acid composition of poultry terrins

2021 ◽  
Vol 30 ◽  
pp. 01014
Author(s):  
Nadezhda Popova ◽  
Yuliya Pisklyukova ◽  
Viktoriya Orlova
Keyword(s):  
Fatty Acid ◽  
Raw Materials ◽  
Raw Material ◽  
Poultry Meat ◽  
Biological Efficiency ◽  
Omega 3 ◽  
Plant Materials ◽  
Poultry Products ◽  
Wide Range ◽  
Omega 6

The development of functional products characterized by high biological efficiency is an urgent task. The article presents the results of the development of balanced-fatty acid terrins from poultry meat. To increase the biological effectiveness of chopped products, raw materials containing omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) were selected. Based on the analysis of the chemical composition of the raw material kit, the intervals for the Introduction of ingredients containing biologically effective components are calculated. Their optimum amount has been experimentally established. The resulting innovative products are characterized by a balanced composition in terms of content and ratio of omega-3 and omega-6 PUFAs. In addition, the value of the indicator of biological value has increased. Developed poultry terrins can be recommended both for a wide range of consumers, and for use in diet (including low-carb diets), baby food. The developed terrines will expand the range of chopped poultry products of functional orientation.

scholarly journals The spectrophotometric determination of A-vitamin activity of carotenoid-containing raw material

2021 ◽  
pp. 17-26
Author(s):  
V.V. Velichko ◽  
◽  
D.S. Kruglov ◽  
Keyword(s):  
Spectrophotometric Determination ◽  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Health Improvement ◽  
Plant Materials ◽  
Plant Raw Materials ◽  
Wide Range ◽  
Vitamin Activity

Carotenoids are of interest as precursors of vitamin A, which has a wide range of pharmacological effects — antioxidant, immunomodulatory, participating in photoreception, etc. The aim of the research was to develop a technique for the spectrophotometric determination of the A-vitamin activity of carotenoids-rich medicinal plant raw materials (MPRM). The objects of research were the traditional sources of carotenoids — sea buckthorn, carrot, pumpkin and wild rose. A method is proposed for determining the content of individual carotenoids in MPRM by optical density at wavelengths corresponding to the characteristic peaks of α-carotene, β-carotene and lycopene. It has been shown that it is preferable to use isopropyl alcohol as the most effective solvent. Taking into account the content of specific carotenoids makes it possible to determine the A-vitamin activity of the sum of carotenoids extracted from plant materials, and, accordingly, to increase the efficiency of the use of herbal preparations and biologically active additives for health improvement.

USE OF ELECTROPHYSICAL METHODS WHEN PROCESSING OIL RAW MATERIALS

2019 ◽  
Author(s):  
И.А. Шорсткий
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
New Technologies ◽  
Raw Materials ◽  
Pulsed Electric Field ◽  
Processing Plant ◽  
Plant Materials ◽  
Literary Sources ◽  
Wide Range ◽  
Processed Products

Представлен обзор передовых технологий в области переработки масличных материалов, способных совмещать традиционные подходы с новыми электрофизическими методами, формируя более совершенный и безопасный процесс. Показано, что электрофизические методы, к которым относят обработку импульсным электрическим полем, СВЧобработку, обработку холодной плазмой, начинают активно использовать для подготовки масличных материалов или для проведения процесса переработки с наложением электрофизических методов. Отмечено, что некоторые из электрофизических методов, в частности СВЧнагрев и обработка импульсным электрическим полем, промышленно апробированы. Приведены особенности их применения. На основе критического анализа литературных источников отмечено, что для пищевой промышленности необходимо развивать современные комбинированные технологии переработки растительных материалов совместно с электрофизическими методами, способствующие сохранению и улучшению питательной ценности получаемых продуктов и повышению эффективности процесса переработки. The review shows that a wide range of new technologies appears in the field of oilseed materials processing, which are able to combine traditional approaches with new methods based on electric fields, forming a more perfect and safer process. It is indicated that methods based on electric fields, which include the processing of a pulsed electric field, microwave processing, cold plasma processing are beginning to be actively used for the preparation of oilbearing materials or for carrying out the process of processing with the imposition of methods based on electric fields. It is noted that some of the methods based on electric fields in particular, microwave heating and pulsed electric field processing have already been industrially tested. The paper also shows the features of their application. On the basis of a critical analysis of literary sources, it is noted that for the food industry it is extremely important to develop modern, combined technologies for processing plant materials together with methods based on electric fields that can preserve and in some cases improve the nutritional value of the processed products and at the same time increase the efficiency of the processing process.

scholarly journals DNA Barcoding for Industrial Quality Assurance

Planta Medica ◽  
2017 ◽  
Vol 83 (14/15) ◽  
pp. 1117-1129 ◽  
Author(s):  
Tiziana Sgamma ◽  
Claire Lockie-Williams ◽  
Marco Kreuzer ◽  
Sarah Williams ◽  
Ulrike Scheyhing ◽  
...  
Keyword(s):  
Quality Control ◽  
Quality Assurance ◽  
Dna Barcoding ◽  
High Throughput Screening ◽  
Raw Materials ◽  
Dna Barcode ◽  
Positive Control ◽  
Herbal Drug ◽  
High Resolution Melt ◽  
Plant Materials

AbstractDNA barcoding methods originally developed for the identification of plant specimens have been applied to the authentication of herbal drug materials for industrial quality assurance. These methods are intended to be complementary to current morphological and chemical methods of identification. The adoption of these methods by industry will be accelerated by the introduction of DNA-based identification techniques into regulatory standards and monographs. The introduction of DNA methods into the British Pharmacopoeia is described, along with a reference standard for use as a positive control for DNA extraction and polymerase chain reaction (PCR). A general troubleshooting chart is provided to guide the user through the problems that may be encountered during this process. Nevertheless, the nature of the plant materials and the demands of industrial quality control procedures mean that conventional DNA barcoding is not the method of choice for industrial quality control. The design of DNA barcode-targeted quantitative PCR and high resolution melt curve tests is one strategy for developing rapid, robust, and reliable protocols for high-throughput screening of raw materials. The development of authentication tests for wild-harvested Rhodiola rosea L. is used as a case study to exemplify these relatively simple tests. By way of contrast, the application of next-generation sequencing to create a complete profile of all the biological entities in a mixed herbal drug is described and its potential for industrial quality assurance discussed.

scholarly journals Studying the possibility of using non-traditional vegetable raw materials of the Republic of Adygea in the production of functional antioxidant products

2021 ◽  
Vol 17 (5) ◽  
pp. 43-52
Author(s):  
A. A. Skhalyakhov ◽  
Kh. R. Siyukhov ◽  
Z. T. Tazova ◽  
L. V. Lunina
Keyword(s):  
Antioxidant Activity ◽  
Raw Materials ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Food Products ◽  
Biologically Active ◽  
Plant Materials ◽  
Amperometric Method ◽  
Wide Range ◽  
The Republic

In the last decade the attitude of socially active people to their own health has changed significantly. The desire to lead a healthy lifestyle forms consumers’ demand for proper balanced nutrition, products enriched with natural biologically active substances of unconventional plant materials, systematic consumption of which can not only reduce the risk of alimentary diseases, but also protect a person from oxidative stress, which is the predecessor of many serious illnesses. Among the wide range of chemical compounds that make up plant materials, a special place is occupied by compounds of antioxidant action, such as flavonoids, phenol carboxylic acids, vitamins C and E. The aim of the research was to study the qualitative composition and quantitative content of natural antioxidants in some types of medicinal and technical plant materials of the Republic of Adygea to determine the possibility of their use in the production of functional food products. The content of biologically active compounds that determine the antioxidant properties of plant materials was determined using «Kapel 105 «M» capillary electrophoresis system and JASCO 875-UV liquid chromatograph. Antioxidant activity was measured by the amperometric method with Tsvet Yauza-01-AA analyzer. According to the results of the research, it has been found that Echinacea purpurea herb (1,09 g / dm3) and walnut leaves (0,96 g / dm3) exhibit a high antioxidant activity (in terms of gallic acid), which is due to the high concentration of natural antioxidants of the phenolic type. Due to these properties this plant raw materials can be used as enriching ingredients for the production of various groups of food products for functional purposes.

Microstructural characterization of laser-melted/roller-quenched dicalcium silicate

1988 ◽  
Vol 46 ◽  
pp. 582-583
Author(s):  
C. J. Chan ◽  
K. R. Venkatachari ◽  
W. M. Kriven ◽  
J. F. Young
Keyword(s):  
Particle Size ◽  
Raw Materials ◽  
Shape Change ◽  
Microstructural Characterization ◽  
Particle Size Effect ◽  
Dicalcium Silicate ◽  
Laser Melting ◽  
Uniform Size ◽  
Cell Shape Change ◽  
Wide Range

Dicalcium silicate (Ca2SiO4) is a major component of Portland cement. It has also been investigated as a potential transformation toughener alternative to zirconia. It has five polymorphs: α, α'H, α'L, β and γ. Of interest is the β-to-γ transformation on cooling at about 490°C. This transformation, accompanied by a 12% volume increase and a 4.6° unit cell shape change, is analogous to the tetragonal-to-monoclinic transformation in zirconia. Due to the processing methods used, previous studies into the particle size effect were limited by a wide range of particle size distribution. In an attempt to obtain a more uniform size, a fast quench rate involving a laser-melting/roller-quenching technique was investigated.The laser-melting/roller-quenching experiment used precompacted bars of stoichiometric γ-Ca2SiO4 powder, which were synthesized from AR grade CaCO3 and SiO2xH2O. The raw materials were mixed by conventional ceramic processing techniques, and sintered at 1450°C. The dusted γ-Ca2SiO4 powder was uniaxially pressed into 0.4 cm x 0.4 cm x 4 cm bars under 34 MPa and cold isostatically pressed under 172 MPa. The γ-Ca2SiO4 bars were melted by a 10 KW-CO2 laser.

Fruit and berry raw materials is a promising source of food ingredients for flour confectionery.

2020 ◽  
Vol 29 (11) ◽  
pp. 45-49
Author(s):  
L.N. Fedyanina ◽  
◽  
E.S. Smertina ◽  
V.A. Lyakh ◽  
A.E. Elizarova ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Scientific Literature ◽  
Consumer Demand ◽  
Raw Materials ◽  
Plant Materials ◽  
Food Ingredients ◽  
Promising Source

The article considers the problem of improving the range of confectionery from the standpoint of use plant materials of satisfaction by consumer demand in dieteticpreventive foods. The analysis of domestic and foreign scientific literature on promising directions of improving the range of dietetic-preventive confectionery is given. It is noted that in the recipes for flour confectionery introduced from non-traditional raw materials containing dietary fiber.

Gluten-Free Dry Mixtures with Rice and Amaranth for Children over Three Years Old with Gluten Intolerance

Food Industry ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 23-31
Author(s):  
Sergey Urubkov ◽  
Svetlana Khovanskaya ◽  
Ekaterina Pyrieva ◽  
Olga Georgieva ◽  
Stanislav Smirnov
Keyword(s):  
Celiac Disease ◽  
Treatment Effectiveness ◽  
Raw Materials ◽  
Calculated Data ◽  
Limited Range ◽  
Gluten Free ◽  
Fruit And Vegetable ◽  
Organoleptic Evaluation ◽  
Free Products ◽  
Wide Range

Diet therapy is one of the main approaches to the treatment of a wide range of diseases of the digestive system. The treatment effectiveness of celiac disease depends on how strictly the patient adheres to a gluten-free diet. It is often disrupted due to the limited range of recommended foods and dishes, especially for children who are particularly sensitive to dietary restrictions. In this case, the development of new types of specialized gluten-free products is relevant, allowing to expand the diet both in terms of nutritional value and taste diversity. This study concerns the recipe developments of dry gluten-free mixtures using rice and amaranth with the inclusion of fruit and vegetable and berry raw materials intended for the nutrition of children over three years old suffering from celiac disease. When developing the recipes, researchers used various combinations of rice and amaranth flour, as well as fruit and vegetable powders. The rice flour composition varied in the range from 15 to 75%; amaranth – from 15 to 45%; fruit and vegetable and berry powders – up to 10%. The finished product was gluten-free cookies, muffins, pancakes made of rice and amaranth. Organoleptic evaluation showed that the studied samples of gluten-free cookies have high quality characteristics, have a pleasant taste and aroma. According to the calculated data, specialized gluten-free dry mixtures intended for children over three years with celiac disease can serve as an important source of: vegetable carbohydrates – from 26.81 to 55.19 g / 100g of finished products; protein – from 4.06 to 11.82 g/100g of finished products; dietary fiber – from 3.82 to 6.36 g/100g of finished products; and energy – from 158.12 to 333.96 kcal/100g of finished products) The developed recipess of gluten-free products can help to provide children with an adequate amount of nutrients and energy.

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