Exogenous application of RNAs as a silencing tool for discovering gene function.
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Abstract This chapter focuses on the importance of the RNA silencing technique in unraveling the function of genes by inhibiting gene expression at the post-transcriptional level, and is particularly appropriate for studying developmental processes such as fruit setting and growth that require a tight organ/tissue and time-specific regulation of the expression of target genes. Some methods used for establishing the function of a specific gene altering gene expression at either the genomic or post-transcriptional level are also presented.
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2013 ◽
Vol 368
(1632)
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pp. 20130022
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2008 ◽
Vol 105
(46)
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pp. 18012-18017
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2021 ◽
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