scholarly journals Response of broilers to graded levels of microbial phytase added to maize–soyabean-meal-based diets containing three levels of non-phytate phosphorus

1996 ◽  
Vol 75 (6) ◽  
pp. 839-852 ◽  
Author(s):  
E. T. Kornegay ◽  
D. M. Denbow ◽  
Z. Yi ◽  
V. Ravindran
Keyword(s):  
Feed Intake ◽  
Control Diet ◽  
Linear Equations ◽  
Basal Diet ◽  
Positive Control ◽  
Phytate Phosphorus ◽  
Microbial Phytase ◽  
Soyabean Meal ◽  
Non Linear Equations ◽  
Total P

Male 1-d-old broilers (n 920) were given 0, 200, 400, 600, 800, 1000 and 1200 U microbial pbytase/kg diet in combination with 2·0, 2·7 or 3·4 g non-phytate P (nP)/kg or 4·0, 5·1 or 5·8 g total P (tP)/kg in a 21 d trial to assess the effectiveness of phytase in a maize–soyabean-meal diet. In addition to the above twenty-one diets, a positive control P diet supplied 4·5 g nP/kg, 6·9 g tP/kg and 10 g Ca/kg. The basal diet contained 230g crude protein/kg, 8·8 g Ca/kg, 4·4 g tP/Fg and 2/0 g nP/kg. Defluorinated phosphate and limestone were used to supply P and Ca. A Ca:tP ratio of 2:l was maintained except in the positive control diet which had a ratio of 1·45: 1. Phytase additions linearly increased (P < 0·01) body-weight (SW) gain, feed intake, toe ash percentage, and apparent retention (% of intake) or total amount (g/bird) of retained Ca and P, and linearly decreased (P < 0·01) P excretion (g/kg of DM intake) at each level of nP with the magnitude of the response inversely related to the level of nP. Above-normal mortality was only observed in the group receiving 2·0 g nP/kg diet without phytase. Adding nP linearly increased (P < 0·01) BW gain, feed intake, toe ash percentage, Ca retention, total amount (g/bird) of P retained, and P excretion, and iinearly decreased (P < 0·01) apparent retention (%) of P. Derived linear and non-linear equations for BW gain and toe ash percentage at the two lower nP levels, 2·0 and 2·7 g/kg, were used to calculate P equivalency value of microbial phytase. The results show that 939 U microbial phytase is equivalent to 1 g P from defluorinated phosphate in broilers fed on maize–soyabean-meal diets. The amount of P released per 100 U phytase decreased as the total amount of phytase increased.

Dietary phosphate equivalence of four forms of Pi contrasted with a novel microbial phytase from Citrobacter braakii in broiler chickens

2015 ◽  
Vol 55 (9) ◽  
pp. 1145 ◽  
Author(s):  
A. J. Cowieson ◽  
F. Fru-Nji ◽  
O. Adeola
Keyword(s):  
Broiler Chickens ◽  
Potassium Phosphate ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Microbial Phytase ◽  
Dietary Phosphate ◽  
Bone Mineralisation ◽  
Tibia Ash ◽  
Total P

Two 21-day studies were conducted with broilers to evaluate the efficacy of a bacterial 6-phytase from Citrobacter braakii and compare four Pi sources. The four sources were phosphates of monocalcium (MCP), dicalcium (DCP), tricalcium (TCP) with potassium phosphate (KH2PO4) acting as a ‘positive control’ reference. In each study, 336 4-day-old male birds (Ross 708) were blocked based on initial bodyweight (BW) and randomly allotted to one of seven diets with six replicate cages of eight birds each. Access to experimental diets and water from Days 4 to 25 post-hatching was ad libitum. In the first study, the seven diets were: (1) a low-P negative control (NC) corn-soybean meal basal diet formulated to contain crude protein (CP), Ca, total P, and non-phytate P (nPP) at 218, 9.0, 4.5, and 2.0 g/kg, respectively; (2) NC plus 0.75 g Pi from KH2PO4/kg; (3) NC plus 0.75 g Pi from MCP/kg; (4) NC plus 0.75 g Pi from DCP/kg; (5) NC plus 0.75 g Pi from TCP/kg; (6) NC plus phytase at 500 FYT/kg; (7) NC plus phytase at 1000 FYT/kg. Feeding the low-P NC diet reduced (P < 0.01) BW gain (BWG), feed intake (FI), and tibia ash. Supplementing the NC with Pi or phytase linearly improved (P < 0.01) BWG, FI, and tibia ash. Supplementing the NC with 0.75 g/kg Pi from MCP, DCP, or TCP were equipotent in improving BWG and FI; however, percent tibia ash was higher (P < 0.05) in birds fed MCP than either DCP or TCP. The second study was similar to the first study except that Ca, total P, and nPP in the NC were reduced to 7.0, 4.2, and 1.8 g/kg, respectively; and Pi from MCP, DCP, and TCP were reduced to 0.6 g/kg. Similar to observations in the first study, the low-P NC diet reduced (P < 0.01) BWG, FI, and tibia ash; and supplementing the NC with Pi or phytase linearly improved (P < 0.01) BWG, FI, P digestibility and tibia ash. Furthermore, supplementing the NC with 0.75 g Pi from MCP, DCP, or TCP per kg diet were equipotent in improving BWG, FI, and tibia ash though MCP resulted in superior (P < 0.05) retention of P compared with TCP. Results from both studies showed that the phytase was efficacious in releasing phytate-P for growth and bone mineralisation in chickens compensating, at least, the spared Pi, and that Pi sources commonly used in formulating diets of chickens may be different in their potential to supply digestible P.

scholarly journals Effect of two microbial phytase preparations on phosphorus utilisation in broilers fed maize-soybean meal based diets

2001 ◽  
Vol 10 (1) ◽  
pp. 19-26 ◽  
Author(s):  
J. VALAJA ◽  
S. PERTTILÄ ◽  
K. PARTANEN
Keyword(s):  
Aspergillus Niger ◽  
Trichoderma Reesei ◽  
Soybean Meal ◽  
Broiler Chickens ◽  
Control Diet ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Inorganic P ◽  
Microbial Phytase

The present study was carried out to determine the effect of two microbial phytases, Aspergillus niger (FINASEâ FP-500, 291 PU (phytase units)/g) and Trichoderma reesei phytase (FINASEâ P, 5880 PU/g) on phosphorus (P) and calcium (Ca) utilisation and ileal P and Ca digestibility in broiler chickens fed diets based on maize and soybean meal. A total of 96 Ross broiler chickens housed four birds to a cage were used. Four dietary treatments consisted of a positive control supplemented with dicalcium phosphate (17 g/kg), a negative control without inorganic P, basal diet without inorganic P supplemented with Aspergillus niger phytase (2.6 g/kg) and basal diet without inorganic P supplemented with Trichoderma reesei phytase (0.13 g/kg). Both phytases provided 750 PU/kg feed. P retention per unit intake was lowest and P excretion highest in birds fed the positive control diet with inorganic P (P

scholarly journals 369 An investigation into the influence of the seaweed extract laminarin on post-weaning pig performance and the intestinal microbiome

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 132-132
Author(s):  
Stafford Vigors ◽  
John O’ Doherty ◽  
Mary McDonnell ◽  
Ruth Rattigan ◽  
Torres Sweeney
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Feed Intake ◽  
Control Diet ◽  
Basal Diet ◽  
Intestinal Microbiome ◽  
Rrna Gene ◽  
Weaned Pigs ◽  
Weaning Pig ◽  
Pig Performance

Abstract Marine macroalgal derivatives such as laminarin have been considered as potential bioactive compounds in swine nutrition to improve post-weaning pig performance. However, a comprehensive understanding of the impact of these extracts on the intestinal microbiome and its subsequent influence on performance is not well characterized. Therefore, the objective of this study was to evaluate the effects of supplementing the diet of newly weaned pigs with laminarin, on animal performance and the intestinal microbiota using 16S rRNA gene sequencing. At 28 days of age, newly weaned pigs (n = 54) were assigned to one of two dietary groups: 1) basal diet and 2) basal diet + 300 ppm laminarin for a 14-day period. On day 15, nine pigs per treatment (one from each pen) were humanely euthanized and high-throughput sequencing of the V3-V4 hypervariable region of the bacterial 16S rRNA gene was performed on the Illumina MiSeq. Pigs fed the laminarin-supplemented diet had increased average daily feed intake, growth rate and body weight compared to pigs fed the control diet (P &lt; 0.05). Pigs fed the laminarin supplemented diet had reduced abundances of Enterobacteriaceae (P &lt; 0.05) compared to pigs fed the control diet. Enterobacteriaceae and Helicobacteraceae had negative relationships with average daily feed intake (ADFI), average daily gain (ADG) and butyric acid concentrations. In contrast, the beneficial Prevotellaceae were positively correlated with ADFI, ADG, total VFA, acetic, propionic, butyric and negatively correlated with isovaleric acid. Hence, supplementation with laminarin may accelerate the adoption of pigs to a post-weaning diet by promoting the proliferation of bacterial species that favourably enhance nutrient digestion and reduce the load of pathogenic bacteria impacting pig performance.

scholarly journals Control of the lipid oxidation in Nile tilapia feed

2016 ◽  
Vol 46 (9) ◽  
pp. 1675-1677 ◽  
Author(s):  
Thiago Luís Magnani Grassi ◽  
Marcelo Tacconi de Siqueira Marcos ◽  
Elisa Helena Giglio Ponsano
Keyword(s):  
Lipid Oxidation ◽  
Nile Tilapia ◽  
Control Diet ◽  
Bacterial Biomass ◽  
Thiobarbituric Acid ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Rubrivivax Gelatinosus ◽  
Experimental Treatments

ABSTRACT: The purpose of this research was to investigate the progress of the rancidity in Nile tilapia diets containing bacterial biomass of Rubrivivax gelatinosus . Six experimental treatments comprised basal diet (negative control), diet with asthaxanthin (positive control) and 4 diets with different concentrations of the bacterial biomass. The thiobarbituric acid assay for rancidity analyses were accomplished after 6 and 12 months of diets storage. It was concluded that Rubrivivax gelatinosus biomass minimized the racidity in Nile tilapia diets in 32.52 to 44.72% at 6 months and in 37.85 to 52.37% at 12 months of storage.

Effect of xylanase supplementation of wheat-based liquid diets, for weaner pigs, steeped for 1 or 24 h before feeding

2013 ◽  
Vol 53 (6) ◽  
pp. 540 ◽  
Author(s):  
Katie A. l'Anson ◽  
Mingan Choct ◽  
Peter H. Brooks
Keyword(s):  
Feed Intake ◽  
Control Diet ◽  
Basal Diet ◽  
Feed Conversion ◽  
Large White ◽  
Available Lysine ◽  
Daily Gain ◽  
Positive Effect ◽  
Weaner Pigs ◽  
Entire Male

Seventy-two individually housed, entire male (Large White × Landrace) weaner pigs (28 days; 7.4 ± 0.4 kg) were used in a 26-day feeding trial. The pigs were allocated to one of six treatments in a 3 by 2 factorial design. The factors were (1) steeping duration of liquid feed [0 (dry control diet), 1 or 24 h], and (2) enzyme supplementation (0 or 300 ppm xylanase). The basal diet was wheat-based (65%) and was formulated to be marginally adequate for pigs of this age, containing 14.5 MJ digestible energy (DE)/kg and 0.85 g available lysine/MJ DE. Feeding pigs liquid diets steeped for 1 h increased feed intake (61 g/day; s.e.d. 17; P < 0.05) and daily gain (70 g/day; s.e.d. 17; P < 0.05) and improved feed conversion ratio (1.16 versus 1.22; s.e.d. 0.03; P < 0.05) compared with pigs fed dry diets. Performance was not further improved by steeping for 24 h. The addition of xylanase to the diets had no positive effect on any of the performance measures and was associated with a significant (40 g/day; s.e.d. 14; P < 0.05) reduction in feed intake. These results provide no support for supplementing liquid diets for weaner pig diets with xylanase.

Influence of selenium source on the performance, feathering and meat quality of broilers

2017 ◽  
Vol 5 ◽  
Author(s):  
V. Ravindran ◽  
S. Elliott
Keyword(s):  
Meat Quality ◽  
Feed Intake ◽  
Sodium Selenite ◽  
Control Diet ◽  
Positive Control ◽  
Negative Control ◽  
Breast Meat ◽  
Growing Period ◽  
Breast Meat Yield

SummaryThe following trial was conducted to investigate the effects of Se source (inorganic sodium selenite (SS) versus organic use Sel-Plex® (SP; Alltech Inc, Nicholasville, KY, USA)) on the performance, feathering and meat quality of 900, one-day-old male broilers over a 42 day growing period. Pens (n = 20) were randomly assigned to either: Diet A (no Se; negative control), Diet B as for diet A plus 0.3 mg/kg SS (positive control), Diet C as for diet A plus 0.2 mg/kg SP, Diet D as for diet A plus 0.3 mg/kg SP and Diet E as for diet A plus 0.4 mg/kg SP. From day 1 to 28, significant (P < 0.05) treatment effects were seen for feed intake and FCR, with values for the negative control being higher than for the diets containing Se. The best FCR was recorded for the diet containing 0.4 mg/kg SP. Breast meat yield for birds fed the negative control were lower than those fed selenite or SP supplemented diets (P < 0.05). Feather measurements on d 14, 28 and 42 of age showed that, although scores became poorer with age (4.9 at 14 d, 4.6 at 28 d and 3.6 at 42 d), they were unaffected (P > 0.05) by dietary Se inclusion. Numeric trends in favour of SP were seen in cooking losses in meat chilled for 24 hours and seven days. Cooking losses in meat frozen for seven days were significantly influenced by Se inclusion, whereby values for birds fed 0.3 and 0.4 mg/kg SP were significantly lower than values for birds fed inorganic selenite (P < 0.05). Tissue Se concentrations were highest for the SP diets. Meat oxidation from the birds fed SP was lower than for the birds fed no Se or SS. The results demonstrated that SP may be more advantageous in improving certain variables of performance and meat quality compared to inorganic sources of Se.

scholarly journals Comparison of broiler performance using Plantain (Plantago lanceolata L.), Bio-Sel-E and commercial diet

2013 ◽  
Vol 42 (2) ◽  
pp. 123-130 ◽  
Author(s):  
R Chacrabati ◽  
R Chowdhury ◽  
S Yesmin ◽  
H Sano ◽  
M Al-Mamun
Keyword(s):  
Feed Intake ◽  
Performance Index ◽  
Control Diet ◽  
Basal Diet ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Conversion ◽  
Commercial Broiler ◽  
Broiler Diet ◽  
The Impact

An experiment was conducted to determine the impact of Plantain (PL), Bio-Sel-E and commercial broiler diet on the growth performance, feed conversion ratio, performance index and carcass yield of broiler. A total of 160 day old broiler chicks (Cobb-500) were randomly distributed into four groups with four replication in each group and having 10 birds in each replication. Basal diet was used for group 1 (control), Basal diet was supplemented with PL (0.5% of DM basis) and Bio-Sel-E (5g/50 birds in drinking water) for group 2 and 3 respectively and for group 4 used commercial broiler diet (Quality Feed Ltd.). Total feed intake was similar among the groups. Feed conversion ratios of PL diet, Bio-Sel-E diet, and commercial broiler diet were comparable, and were lower (P<0.05) than the control diet. Significantly higher body weight gain and performance index were found in PL, Bio-Sel-E and commercial broiler diet groups than control diet. The dressing yield of PL diet and commercial broiler diet were comparable, and they were higher (P<0.05) than Control diet and Bio-Sel-E diet. It could be concluded that the Supplementation of 0.5% PL in the diet had positive effect on growth, feed intake, feed conversion ratio, performance index and carcass characteristics of broilers almost similar of Bio-Sel-E and commercial broiler diet. DOI: http://dx.doi.org/10.3329/bjas.v42i2.18492 Bang. J. Anim. Sci. 2013. 42 (2): 123-130

scholarly journals Application of a kinetic model to describe phosphorus metabolism in pigs fed a diet with a microbial phytase

2010 ◽  
Vol 148 (3) ◽  
pp. 277-286 ◽  
Author(s):  
R. S. DIAS ◽  
S. LOPEZ ◽  
J. A. MOREIRA ◽  
M. SCHULIN-ZEUTHEN ◽  
D. M. S. S. VITTI ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Specific Radioactivity ◽  
Negative Relationship ◽  
Basal Diet ◽  
Growing Pigs ◽  
Strongly Correlated ◽  
Microbial Phytase ◽  
Liver And Kidney ◽  
P Balance ◽  
Total P

SUMMARYThe objective of the current study was to apply the Vitti–Dias model to investigate phosphorus (P) metabolism in growing pigs fed a diet supplemented with microbial phytase. The basal diet contained maize, defatted rice bran, vegetable oil, soybean meal, limestone, salt and a vitamin and mineral mix. There was no inorganic P in the diet and phytase was added at levels of 253, 759, 1265 and 1748 phytase units (PU)/kg of feed. The compartmental model included four pools of P: (1) gut lumen, (2) plasma, (3) bone and (4) soft tissue. A single dose of 32P was administered, and specific radioactivity was measured in plasma, faeces, bone and soft tissue (muscle, heart, liver and kidney) at different times post-dosing for calculation of P flows between pools. Total P absorbed showed a negative relationship with total P excreted in faeces and was strongly correlated with bone P retention, suggesting that absorbed P was channelled to bone to address its physiological growth. Average efficiency of metabolic utilization of absorbed P was estimated to be 0·94, with 0·52 g/g of total net P balance being accreted in bone and the rest in soft tissue (including muscle and some vital organs). The Vitti–Dias model provided suitable representation of P interchange between compartments (in particular, flows between gut and plasma and partitioning of available P between bone and soft tissue), resulting in estimates of P flows comparable with values calculated from balance data.

The effect on nitrogen retention in lambs of feeding protein supplements direct to the abomasum. Comparisonof liquid and dry feeding and of various sources of protein

1969 ◽  
Vol 73 (3) ◽  
pp. 469-475 ◽  
Author(s):  
E. R. Ørskov ◽  
C. Fraser
Keyword(s):  
Fish Meal ◽  
Control Diet ◽  
Nitrogen Retention ◽  
Basal Diet ◽  
Regression Coefficients ◽  
Sunflower Meal ◽  
Protein Supplements ◽  
Liquid Suspension ◽  
Soyabean Meal ◽  
Urinary Nitrogen

SummaryThree experiments were conducted to investigate the influence on nitrogen retention of feeding protein supplements to early weaned lambs direct to the abomasum via the oesophageal groove. In Exp. 1 the lambs received a basal diet of barley and a protein mixture consisting of soyabean meal, sunflower meal and fish meal which was given either in a liquid suspension from a bottle or as dry feed incorporated with the barley. The results showed that the regression coefficients of nitrogen retention on intake were 0.48 and 0.33 with liquid and dry feeding respectively. The regressions of urinary nitrogen on intake were with liquid and dry feeding respectively 0.26 and 0.44.In Exp. 2, various sources of protein were compared isonitrogenously as substitute for part of a barley concentrate ration. The nitrogen intakes and retentions were 14·28 and 5·09, 20·16 and 9·39, 20·53 and 8·19, 19·00 and 7·80, and 18·87 and 7·86 g/day for the control diet and fish meal, yeast, soyabean meal and sunflower meal supplemented diets respectively.In Exp. 3 the fish meal supplement was compared with a high lysine, bloodmeal and with casein as liquid supplements to a basal diet of barley concentrate. The intake and retention of nitrogen were for bloodmeal, casein and fish meal respectively 23·41 and 7·48, 22·56 and 9·11, and 21·88 and 8·31 g/day. It appeared that the protein sources high in methionine resulted in the highest nitrogen retention. The implications of these findings in relation to the nutrition of young ruminants is discussed together with an evaluation of the experimental method as a technique to study postruminal digestion.

scholarly journals Effects of supplemental microbial phytase on performance of broiler chickens

1994 ◽  
Vol 3 (5) ◽  
pp. 457-466
Author(s):  
Tuomo Koskinen ◽  
Jari Piironen ◽  
Tiina Hakonen
Keyword(s):  
Broiler Chickens ◽  
Bone Mineralization ◽  
Control Diet ◽  
Live Weight ◽  
Positive Control ◽  
Negative Control ◽  
Phytase Activity ◽  
Broiler Chicks ◽  
Microbial Phytase ◽  
Soya Bean Meal

Three trials were conducted on a total of 5100 broiler chicks (0-5.5 weeks) to study the effects of different microbial phytase (Aspergillus niger) supplementations (250-1000 phytase units = PU/g) on the performance and bone mineralization of birds and on the utilization of phosphorus compared with the effects of mineral P additions as dicalcium phosphate. The basal diets (negative controls) were principally composed of soya bean meal (SBM) and grain (wheat, barley, oats) supplemented with up to 0.10% mineral P; the positive control diets were supplemented with 0.24-0.30% mineral P. Compared with the negative control groups, which were fed diets with 0.05% mineral P either during the whole rearing period or during the starting period only, phytase addition increased live weight by 4-7% and feed intake by 3-9% (Trials 1 and 2). Tibia values indicated that with low mineral P supplementations (0.05 and 0.10%), phytase additions have a non-significant effect on bone mineralization. If mineral P is not added, the tibia values show a marked response to phytase (Trial 3). Utilization of P increased from 50.9% to 60.0% when phytase (1000 PU/g) was added to the diets without mineral P supplementation (Trial 3). Utilization of P was only 34.5% in the positive control diet (0.3% mineral P). The results were obviously affected by the intrinsic phytase activity in grain and the dietary calcium level, and suggest that, in SBM-grain-based diets with very low (0.05%) or no mineral P supplementation, weight gain can be increased by adding phytase up to 1000 PU/g. This supplemented phytase activity is apparently sufficient for adequate bone mineralization.

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