scholarly journals THE PREPARATION AND PROPERTIES OF INFLUENZA VIRUS VACCINES CONCENTRATED AND PURIFIED BY DIFFERENTIAL CENTRIFUGATION

1945 ◽  
Vol 81 (2) ◽  
pp. 193-218 ◽  
Author(s):  
W. M. Stanley
Keyword(s):  
Influenza Virus ◽  
Ultraviolet Light ◽  
Large Scale ◽  
Allantoic Fluid ◽  
Red Cell ◽  
Differential Centrifugation ◽  
Frozen State ◽  
Elution Method ◽  
Better Than

Influenza virus vaccines containing from 1 to 10 mg. of virus materials per cc. concentrated and purified from infectious allantoic fluids by means of one or two cycles of differential centrifugation and inactivated by different treatments have been prepared and subjected to laboratory tests. Suitable inactivation of the virus preparations with retention of full red cell agglutinating activity and immunizing potency in mice was achieved by treatment with minimal amounts of formaldehyde or ultraviolet light. Treatment with phenol or chloroform failed to cause adequate loss of virus activity. Excessive amounts of formaldehyde or of ultraviolet light were found to cause a loss in red cell agglutinating activity and in immunizing potency. Freezing resulted in the immediate loss of red cell agglutinating activity of the formalinized vaccine. Storage of the vaccines in the frozen state was accompanied by a gradual decrease in red cell agglutinating activity. Drying of the vaccines from the frozen state resulted in a loss of red cell agglutinating activity and, in the case of the formalinized vaccine, in a loss in immunizing potency. There appeared to be at least a rough correlation between red cell agglutinating activity and immunizing potency. The immunizing potency and red cell agglutinating activity of a purified formalinized vaccine containing 2 mg. of virus material per cc. were unchanged following 2 months' storage at 4° but were measurably decreased following storage for 2 months at 18 to 25° and at 37°. At equivalent dosages of virus material the immunizing potency of formalinized centrifugally purified virus, of formalinized virus purified by the red cell elution method, and of infectious allantoic fluid was not measurably different. The immunizing potency of a formalinized polyvalent vaccine containing centrifugally purified Lee, PR8, and Weiss influenza virus materials at concentrations of 5, 2.5, and 2.5 mg. per cc., respectively, was found to be essentially the same as that of a similar vaccine prepared commercially. In both cases the protection afforded against the Weiss strain appeared to be better than that against the Lee and PR8 strains. The commercially prepared vaccine is being subjected to clinical tests in man at dosage levels ranging from 0.01 mg. to 10 mg. The latter corresponds to a level approximately 100 times that of infectious allantoic fluid. It was found that the bacterial contamination that frequently accompanies operation on a large scale can be controlled by the addition of one part per 10,000 of formalin plus one part per 100,000 of phenyl mercuric nitrate to the allantoic fluid immediately following harvesting, without affecting the quality of the vaccine. This procedure and the use of virus materials purified and concentrated by a single cycle of differential centrifugation by means of the Sharples centrifuge were found to be suitable for the production of influenza virus vaccines on a large scale. By means of this method influenza vaccines possessing 20 or more times the immunizing potency of infectious allantoic fluid and 10 or more times the immunizing potency of the usual commercial vaccine prepared by the red cell elution method can be manufactured rapidly on a very large scale with considerable ease and efficiency.

scholarly journals AN EVALUATION OF METHODS FOR THE CONCENTRATION AND PURIFICATION OF INFLUENZA VIRUS

1944 ◽  
Vol 79 (3) ◽  
pp. 255-266 ◽  
Author(s):  
W. M. Stanley
Keyword(s):  
Influenza Virus ◽  
Large Scale ◽  
Allantoic Fluid ◽  
Red Cells ◽  
Virus Protein ◽  
Differential Centrifugation ◽  
Freezing And Thawing ◽  
Protein Nitrogen ◽  
Adult Chicken ◽  
Sedimentation Constant

The concentration and purification of influenza virus by means of differential centrifugation in a vacuum type centrifuge, by adsorption on and elution from adult chicken red cells, by elution of the precipitate formed on freezing and thawing of allantoic fluid, by adsorption on and elution from embryonic chick red cells, and by combinations of the first method with each of the three succeeding methods, have been studied. Over-all yields of virus of about 50 to 70 per cent were obtained by these methods and combinations of methods except for somewhat lower yields when adsorption on and elution from adult chicken red cells was employed. However, the purified products obtained by methods involving only the use of red cells or the freezing and thawing technique were found to contain about 80 per cent of non-virus protein. The purified products obtained when differential centrifugation was used either alone or in combination with any one of the other methods were found to be indistinguishable and to consist of a fairly homogeneous component having a sedimentation constant of about 600 S. Such preparations possessed about 22,000 chicken red cell agglutinating units per mg. of protein nitrogen and solutions containing only about 10–14 gm. of the materials gave 50 per cent infectivity end points in chick embryos. The Sharples centrifuge was found to be almost as efficient as the vacuum type centrifuge for the concentration and purification of influenza virus and, because of its larger capacity, is recommended for the preparation of purified virus on a large scale.

scholarly journals Design and Demonstration of Large Scale Cu2O Photocathodes with Metal Grid Structure for Photoelectrochemical Water Splitting

Energies ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 7422
Author(s):  
Min-Kyu Son
Keyword(s):  
Water Splitting ◽  
Large Scale ◽  
Grid Structure ◽  
Large Area ◽  
Intrinsic Conductivity ◽  
Metal Grid ◽  
Splitting System ◽  
Insight Into ◽  
Better Than

Upscaling of photoelectrode for a practical photoelectrochemical (PEC) water splitting system is still challenging because the PEC performance of large-scale photoelectrode is significantly low, compared to the lab scale photoelectrode. In an effort to overcome this challenge, sputtered gold (Au) and copper (Cu) grid lines were introduced to improve the PEC performance of large-scale cuprous oxide (Cu2O) photocathode in this work. It was demonstrated that Cu grid lines are more effective than Au grid lines to improve the PEC performance of large-scale Cu2O photocathode because its intrinsic conductivity and quality of grid lines are better than ones containing Au grid lines. As a result, the PEC performance of a 25-cm2 scaled Cu2O photocathode with Cu grid lines was almost double than one without grid lines, resulting in an improved charge transport in the large area substrate by Cu grid lines. Finally, a 50-cm2 scaled Cu2O photocathode with Cu grid lines was tested in an outdoor condition under natural sun. This is the first outdoor PEC demonstration of large-scale Cu2O photocathode with Cu grid lines, which gives insight into the development of efficient upscaled PEC photoelectrode.

Time Orientation: Past, Present, and Future Perceptions

1989 ◽  
Vol 64 (3_suppl) ◽  
pp. 1199-1205 ◽  
Author(s):  
Leonard A. Jason ◽  
Jennifer Schade ◽  
Louise Furo ◽  
Arne Reichler ◽  
Clifford Brickman
Keyword(s):  
Quality Of Life ◽  
Religious Orientation ◽  
Large Scale ◽  
Time Orientation ◽  
Nuclear War ◽  
Future Expectations ◽  
The Past ◽  
Future Perceptions ◽  
Better Than

A survey was conducted to assess people's time orientation or where they spend most of their thinking time: past, present or future. 100 women were also asked about their expectations for the quality of life in 20 co 30 yr. and about the odds of a large-scale nuclear war within 30 yr. Respondents thought almost twice as much about the present and future as the past. They rated the quality of life in 20 to 30 yr. as being the same as or slightly better than now. A nuclear war within 30 yr. was considered possible; religious orientation had a strong effect. No significant relationship was found between time orientation and future expectations.

scholarly journals Biochemical Analysis Of Some Dried Sis Fishes Of The River Padma In Rajshahi

1970 ◽  
Vol 6 ◽  
pp. 39-43 ◽  
Author(s):  
Sabina Sultana ◽  
Selina Parween ◽  
M Altaf Hossain
Keyword(s):  
Large Scale ◽  
Biochemical Analysis ◽  
Good Condition ◽  
Calcium Content ◽  
Mystus Vittatus ◽  
Time Period ◽  
Dried Fish ◽  
Glossogobius Giuris ◽  
Better Than

Seven different species viz. Chanda baculis, Chanda ranga, Amblypharyngodon mola, Oxygaster bacaila, Clupisoma atherinoides, Corica soborna, Mystus vittatus and a group of mixed SIS fishes viz. Mastacembelus pancalus, Xenntodon cancila, Chanda baculis and Glossogobius giuris were used for preparation of dust which can be preserved for a time period. The fishes were sun dried or oven dried, which are also method of preservation. Quality of the oven-dried fish was better than that of the sun-dried fish, but sun-drying process is easy and can be used in large scale. The fish powder remained in good condition for 7-9 months at normal room temperature, but at -18°C the powder was in good condition throughout the year. Highest quantity of powder from 1 kg of fish was obtained in case of the mixed species as 24.61% and the lowest in O. bacaila which was 20.52%. Biochemical analysis showed that the maximum calcium content was found as 1.34% in M. vittatus and minimum was 0.80% in mixed SIS fishes. Maximum phosphorus content was 2.90% in C. ranga and minimum was 1.72% in C. soborna. Maximum iron content was found as 45.20 mg/100g in mixed SIS fishes and minimum was found as 16.85 mg/100g in O. bacaila. The maximum moisture content was found in C. ranga (13.50%) and the minimum in mixed SIS fishes (11.65%). The maximum protein content was recorded in the mixed SIS fishes (72.45%) and the minimum in C. ranga (52.65%). The experiment was replicated three times and conducted from July 2005 to July 2008. DOI: http://dx.doi.org/10.3329/jles.v6i0.9719 JLES 2011 6: 39-43

scholarly journals A SEDIMENTABLE COMPONENT OF ALLANTOIC FLUID AND ITS RELATIONSHIP TO INFLUENZA VIRUSES

1944 ◽  
Vol 80 (2) ◽  
pp. 83-100 ◽  
Author(s):  
C. A. Knight
Keyword(s):  
Influenza Virus ◽  
Rabbit Antiserum ◽  
Allantoic Fluid ◽  
Influenza Viruses ◽  
Red Cell ◽  
Serological Tests ◽  
Cell Agglutination ◽  
Small Component ◽  
Serum Dilution ◽  
Neutralizing Capacity

Macromolecular material was isolated from normal allantoic fluid by a centrifugation procedure comparable to that currently employed for the concentration and purification of influenza viruses. The yield of material was found to vary with the age of the embryo, reaching a maximum average value after 14 days of incubation at 39°C. of about 0.02 mg. per ml. of allantoic fluid. The purified material was found to contain protein, carbohydrate, and lipid and to have a general composition similar to purified preparations of PR8 influenza virus. A typical preparation of normal material had an isoelectric point at pH 2.3. Sedimentation studies indicated that the normal material can give a variety of sedimentation constants depending upon the concentration and viscosity of the preparations. The sedimentation constant, corrected for viscosity, of the major component of a fresh preparation was 170 S. The diameters of the predominant particles shown in electron micrographs of the normal material and of preparations of PR8 influenza virus were about 40 and 100 mµ, respectively. Serological tests indicated that the normal material is a good antigen and that preparations of both A and B types of influenza virus obtained from allantoic fluids by centrifugation show a strong serological relationship to the normal material. Freezing and thawing of allantoic fluid, and repeated adsorption of virus on red cells, failed to provide a practical basis for the separation of normal protein from the virus entity in the case of PR8 virus. In the cases of similar preparations of F12 and of Lee viruses, a partial separation of a small component was accomplished by fractional centrifugation and this component and the normal protein were shown to be identical or very closely related. Antiserum to the purified normal material inhibited red cell agglutination by A and B types of influenza virus at serum dilutions of 600 to 700, but failed to show significant neutralizing capacity in chick embryo and in mouse tests at a serum dilution of 100. Rabbit antiserum to purified preparations of PR8 virus gave a 50 per cent red cell agglutination inhibition endpoint at a serum dilution of 112,000. Some of the implications of the findings are discussed.

Simulating SST Teleconnections to Africa: What is the State of the Art?

2013 ◽  
Vol 26 (15) ◽  
pp. 5397-5418 ◽  
Author(s):  
David P. Rowell
Keyword(s):  
Large Scale ◽  
State Of The Art ◽  
Coupled Model ◽  
The State ◽  
Sub Saharan Africa ◽  
Intercomparison Project ◽  
Rainfall Anomalies ◽  
Sub Saharan ◽  
Better Than

Abstract This study provides an overview of the state of the art of modeling SST teleconnections to Africa and begins to investigate the sources of error. Data are obtained from the Coupled Model Intercomparison Project (CMIP) archives, phases 3 and 5 (CMIP3 and CMIP5), using the “20C3M” and “historical” coupled model experiments. A systematic approach is adopted, with the scope narrowed to six large-scale regions of sub-Saharan Africa within which seasonal rainfall anomalies are reasonably coherent, along with six SST modes known to affect these regions. No significant nonstationarity of the strength of these 6 × 6 teleconnections is found in observations. The capability of models to represent each teleconnection is then assessed (whereby half the teleconnections have observed SST–rainfall correlations that differ significantly from zero). A few of these teleconnections are found to be relatively easy to model, while a few more pose substantial challenges to models and many others exhibit a wide variety of model skill. Furthermore, some models perform consistently better than others, with the best able to at least adequately simulate 80%–85% of the 36 teleconnections. No improvement is found between CMIP3 and CMIP5. Analysis of atmosphere-only simulations suggests that the coupled model teleconnection errors may arise primarily from errors in their SST climatology and variability, although errors in the atmospheric component of teleconnections also play a role. Last, no straightforward relationship is found between the quality of a model's teleconnection to Africa and its SST or rainfall biases or its resolution. Perhaps not surprisingly, the causes of these errors are complex, and will require considerable further investigation.

Effective optimal dismantling strategy for interdependent networks based on residual theory

2019 ◽  
Vol 30 (11) ◽  
pp. 1950082
Author(s):  
Wenjing Song ◽  
Sanyang Liu ◽  
Yiguang Bai
Keyword(s):  
Large Scale ◽  
Single Layer ◽  
Selection Mechanism ◽  
Selection Algorithm ◽  
Node Selection ◽  
Research Attention ◽  
Interdependent Networks ◽  
Network Layers ◽  
Better Than

Because of interdependence between different network layers, interdependent networks are more fragile than single-layer networks, and large-scale iterative paralysis occurs easily. How to seek nodes whose removal can effectively dismantle networks has attracted great research attention. In this paper, a novel optimal dismantling strategy Maximum Entropy Centrality (EC) and overlapping betweenness (OB) based on residual theory (ECOB) is proposed. In the ECOB, the residual theory is used to detect the highest influence nodes according to the quality of the residual networks. In addition, to make sorting more accurate, EC and OB parameters are both considered in the node selection mechanism. Simulation shows that the ECOB strategy performs much better than existing methods both in artificial interdependent networks and real-world interdependent networks. This is thanks to the introduced ECOB node selection algorithm with proper parameter criterions.

Kepuasan Masyarakat Terhadap Pelayanan Kelurahan Setelah Pemekaran Di Tangerang Selatan

Liquidity ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 151-159
Author(s):  
Pitri Yandri
Keyword(s):  
Public Perception ◽  
Service Personnel ◽  
Urban Services ◽  
Hospitality Workers ◽  
Care Workers ◽  
Before And After ◽  
Cartesian Diagram ◽  
Better Than

The purpose of this study is (1) to analyze public perception on urban services before and after the expansion of the region, (2) analyze the level of people's satisfaction with urban services, and (3) analyze the determinants of the variables that determine what level of people's satisfaction urban services. This study concluded that first, after the expansion, the quality of urban services in South Tangerang City is better than before. Secondly, however, public satisfaction with the services only reached 48.53% (poor scale). Third, by using a Cartesian Diagram, the second priority that must be addressed are: (1) clarity of service personnel, (2) the discipline of service personnel, (3) responsibility for care workers; (4) the speed of service, (5) the ability of officers services, (6) obtain justice services, and (7) the courtesy and hospitality workers.

Assessment of quality of life in patients with the effects of transient ischemic stroke

2016 ◽  
pp. 37-39
Author(s):  
A. Babirad
Keyword(s):  
Quality Of Life ◽  
Ischemic Stroke ◽  
Large Scale ◽  
Cerebrovascular Diseases ◽  
Control Measures ◽  
Sociological Research ◽  
Cerebrovascular Events ◽  
Sf 36 ◽  
Angioplasty And Stenting

Cerebrovascular diseases are a problem of the world today, and according to the forecast, the problem of the near future arises. The main risk factors for the development of ischemic disorders of the cerebral circulation include oblique and aging, arterial hypertension, smoking, diabetes mellitus and heart disease. An effective strategy for the prevention of cerebrovascular events is based on the implementation of large-scale risk control measures, including the use of antiagregant and anticoagulant therapy, invasive interventions such as atheromectomy, angioplasty and stenting. In this connection, the efforts of neurologists, cardiologists, angiosurgery, endocrinologists and other specialists are the basis for achieving an acceptable clinical outcome. A review of the SF-36 method for assessing the quality of life in patients with the effects of transient ischemic stroke is presented. The assessment of quality of life is recognized in world medical practice and research, an indicator that is also used to assess the quality of the health system and in general sociological research.

Implementasi Voip Server Berbasis IPV6 Dengan Raspberry PI

2019 ◽  
Vol 9 (01) ◽  
pp. 47-54
Author(s):  
Rabbai San Arif ◽  
Yuli Fitrisia ◽  
Agus Urip Ari Wibowo
Keyword(s):  
Internet Protocol ◽  
Wireless Transmission ◽  
Raspberry Pi ◽  
Test Results ◽  
Average Delay ◽  
Ipv6 Network ◽  
Ip Network ◽  
Selection Of ◽  
Better Than

Voice over Internet Protocol (VoIP) is a telecommunications technology that is able to pass the communication service in Internet Protocol networks so as to allow communicating between users in an IP network. However VoIP technology still has weakness in the Quality of Service (QoS). VOPI weaknesses is affected by the selection of the physical servers used. In this research, VoIP is configured on Linux operating system with Asterisk as VoIP application server and integrated on a Raspberry Pi by using wired and wireless network as the transmission medium. Because of depletion of IPv4 capacity that can be used on the network, it needs to be applied to VoIP system using the IPv6 network protocol with supports devices. The test results by using a wired transmission medium that has obtained are the average delay is 117.851 ms, jitter is 5.796 ms, packet loss is 0.38%, throughput is 962.861 kbps, 8.33% of CPU usage and 59.33% of memory usage. The analysis shows that the wired transmission media is better than the wireless transmission media and wireless-wired.

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