PSVIII-20 Heterologous lactobacilli feed additive impact on microflora in Cobb-500 broiler laying hens

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 429-429
Author(s):  
Anastasiia A Kurilova ◽  
Larisa Y Karpenko ◽  
Anna B Balykina ◽  
Alesya A Bakhta ◽  
Ivan I Kochish ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Laying Hens ◽  
Probiotic Bacteria ◽  
Feed Additive ◽  
State University ◽  
Feed Supplement ◽  
Russian Science ◽  
Hatching Eggs ◽  
Experimental Group ◽  
Subsequent Administration

Abstract Experiment was carried out on introducing eggshells from an experimental group of laying hens of lactobacilli strains with anti-C.jejuni activity. Eggs were incubated for 21 days to obtain broiler chickens containing auto-strains of lactobacilli with anti-C.jejuni activity in the blind processes of the intestine. It should be noted that most of the incubation eggs were observed in the 2nd group, in which the eggs were laid for subsequent administration of probiotic bacteria. Check was carried out for the presence of auto-strains of lactobacilli with anti-C.jejuni activity in the intestines of broiler chickens and the timing of their persistence for 1–35 days. The objective of the study was a comparative analysis of the following DNA samples: DNA from a feed supplement containing strains of lactobacilli with anti-C.jejuni activity; DNA samples from litter of laying hens of the parent herd receiving heterologous lactobacilli as part of the feed; isolated DNA from washes from hatching eggs; DNA samples from the contents of the digestive tract of chicken embryos; DNA samples from the intestines of broiler chickens. DNA samples from groups of birds and their embryos obtained without the use of heterologous lactobacilli were used as controls. The microflora of the contents of blind processes was taken from chickens on the 22nd day and on the 35th day of growing. In total, 343 different bacterial OTUs were identified in this study, belonging to 13 types, 26 classes, 51 orders, 106 families and 172 genera. Assessment of the biodiversity of bacterial ensembles in the samples of the contents of blind processes of chickens revealed fundamental differences already at the level of growing time and type. The study was performed at the FGBOU VO “St. Petersburg State University of Veterinary Medicine» with the aid of the Russian Science Foundation Grant (Project No. 18-76-10017).

VETERINARY HYGIENIC EXAMI-NATION OF THE QUALITY OF CHICKENS EGGS WHEN USING THE FOOD ADDITIVE “PRINAROVSKAYA”

2020 ◽  
Vol 3 ◽  
pp. 46-52
Author(s):  
E.M. Belorusskaya ◽  
◽  
A.F. Kuznetsov ◽  
A.J. Nechaev ◽  
I.V. Ivanova ◽  
...  
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Laying ◽  
Feed Additive ◽  
Egg Mass ◽  
Important Indicator ◽  
Shell Mass ◽  
Hatching Eggs ◽  
Experimental Group

The aim of the studies was to conduct a veterinary-hygienic assessment of changes in the eggs productivity of laying hens, after the use of the “Prinarovskaya supplement of fish (DKR)” in their main diet, which is a homogeneous dry mass made from chilled river fish, by-products of fish processings, as well as grain products (wheat bran). The method of production of the additive in-cludes the following steps: grinding, cavita-tion heating and subsequent drying of the resulting mixture. The studied supplement contains a number of amino acids, vitamins and minerals, etc. The object of the study were laying hens of the LomannLsn-Classic breed at the age of 131 days. “Prinarovskaya DKR” were introduced into the diet of ex-perimental laying hens: of the 1st experi-mental group 8 g of “Prinarovskaya DKR” were introduced per 100 g of the main ration (RR); The 2nd experimental group - 6 g. DKR to main ration; 3rd experimental group - 4 g. DKR to main ration; the control group was fed only main ration - feed PK1-1G_1227 (GOST: R 51851-2001). A veteri-nary-hygienic assessment of the quality of eggs, obtained from laying hens, when feed-ing a new feed additive “Prinarovskaya” was carried out. We kept daily records of laid eggs by groups, the period of the beginning of oviposition. The egg production were calculated of the initial and middle laying hens , the egg production intensity for 120 days. To assess egg productivity, the weight of laid eggs, the egg mass for a certain peri-od of egg laying, and the feed costs per 10 laid eggs were determined. Organoleptic, physical, and chemical methods were used to evaluate egg quality. Egg mass is an important indicator of egg nutrition. GOST 31654-2012 "Food eggs. Technical conditions ”, as well as the requirements for chicken hatching eggs, the minimum weight of food and hatching eggs (45 g) is indicated, below which the imple-mentation and incubation of eggs is not rec-ommended [1]. In studies, the following indicators were taken into account: large and small diameter of eggs, egg density, egg shape index of laying hens of different groups. To assess the state of the internal contents of the egg after opening, the follow-ing indicators were taken into account: pro-tein mass, yolk mass, shell mass, egg vol-ume. An external examination of the eggs as-sessed the condition of the shell, the pres-ence of cracks, growths and depressions. The study indicates a positive effect of the feed additive "Prinarovskaya" on the egg produc-tivity of birds in all periods of egg laying

Probiotic fodder additives based on lactobacilles in the diets of meat breed chicken

2020 ◽  
Vol 1 (12) ◽  
pp. 105-111
Author(s):  
E. A. Kapitonova ◽  
◽  
I. N. Nikonov ◽  
M. V. Selina ◽  
◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Egg Production ◽  
Laying Hens ◽  
Poultry Manure ◽  
Egg Laying ◽  
Feed Additive ◽  
Single Application ◽  
Positive Effect ◽  
Experimental Group

This article reflects the results of a study on feeding an experimental group of laying hens for 1–14 days with full-fledged compound feed, additionally containing heterologous lactobacilli with anti-C. jejuni activity as a feed additive. Data were obtained on the sowing rate of target lactobacilli from poultry manure and eggs. The eggs were collected for the production of broiler chickens. As a result of the studies, it was concluded that the use of a feed additive based on heterologous lactobacilli contributed to an increase in egg production per hen – by 7,8–10%, and egg-laying intensity – by 5,7–7,3%. The addition of the additive had a zootechnical effect even after a single application. The introduction of a feed additive based on lactobacilli, in general, had a positive effect on the productivity of the parent hens.

scholarly journals Оцінка безпечності кормової добавки Ліатоксил для курчат-бройлерів

2017 ◽  
Vol 19 (82) ◽  
pp. 137-140
Author(s):  
T.R. Levytskyy
Keyword(s):  
Broiler Chickens ◽  
Hematological Parameters ◽  
Total Duration ◽  
Feed Additives ◽  
Recommended Dose ◽  
Feed Additive ◽  
Control Group ◽  
Feed Supplement ◽  
Internal Organs ◽  
Experimental Group

The research was conducted on the basis of the vivarium of the State Scientific-Research Control Institute of Veterinary Preparations and Feed Additives. To carry out researches, a feed additive of Lyophthixil produced by Sanofort-P was used. Feed supplement Lytaxyl is a powder of gray color with a specific odor. The composition of the feed supplement includes zeolite, bentonite, silicon oxide, fumaric acid, citric acid, and succinic acid. Research liatoksil feed additive on the safety for the target species – to broiler chickens. Research conducted by 30 heads of broiler chickens of 8-day age of which was formed three groups of 10 goals each. The total duration of the experiment – 35 days. Scheme trials included three groups: a control group that received no feed additives; the first experimental group that received the recommended dose; second experimental group that received feed supplement at a dose which is 10 times the recommended dose. For targets were: the general state bird, bird preservation, hematological parameters. At the end of the experiment studied the internal organs. As a result of studies found that the introduction of a feed additive Liatoksil feed for broiler chickens at a dose of 20 g/kg of feed has no negative influence on the general condition, survival, growth and development, hematological indices and state of the internal organs of broiler chickens. Feed additive liatoksil has a sufficient margin of safety and is safe for broiler chickens five times the recommended dose. 

scholarly journals Бактеріальні показники продуктів забою курчат-бройлерів у разі збагачення раціону наномікроелементною кормовою добавкою «Мікростимулін» в аспекті ветеринарно-санітарної експертизи

2015 ◽  
pp. 93-99
Author(s):  
І. В. Яценко ◽  
В. М. Кириченко
Keyword(s):  
Broiler Chickens ◽  
Broiler Chicken ◽  
Bacterial Contamination ◽  
Feed Additive ◽  
Control Group ◽  
Pathogenic Microorganisms ◽  
Not Given ◽  
Number Of Microorganisms ◽  
Experimental Group

Проаналізовано бактеріальну контамінацію умовно-патогенними та патогенними мікроорганізмамипродуктів забою курчат-бройлерів (білі і червонім’язи, серце, печінка, м’язова частина шлунку) в разізбагачення раціону наномікроелементною кормовоюдобавкою (НМКД) «Мікростимулін» протягом чоти-рьох діб зберігання за температури 0–4 оС. Встанов-лено, що найменший рівень МАФАнМ, БГКП, бакте-рій роду Proteus реєструвався в 3-й дослідній групі,курчатам-бройлерам якої задавали з водою 20 см3/дм3НМКД «Мікростимулін» у всіх досліджуваних продук-тах забою. Найбільший рівень МАФАнМ, БГКП, бак-терій роду Proteus реєструвався в контрольній групі,курчатам-бройлерам якої не задавали НМКД «Мікро-стимулін» з водою. Доведено залежність між дозоюНМКД «Мікростимулін» і кількістю мікроорганізміву продуктах забою птиці. The bacterial contamination of broiler chicken slaughter products (white and red muscles, heart, liver, muscular stomach) by conditionally pathogenic and pathogenic microorganisms when the ration of the chickens was enriched by nanomicroelement feed additive (NMFA) «Microstimulin» for four days of storage at 0–4 ºC has been analyzed. It has been found out that the lowest level of MAFAnM, BGIR, bacteria Proteus in all slaughter products under investigation was registered in the 3rd experimental group the broiler chickens of which were given 20 cm³/dm³ NMFA «Microstimulin» with water. The highest level of MAFAnM, BGIR, bacteria Proteus was registered in the control group the broiler chickens which were not given NMFA «Microstimulin» with water. The dependence between the dose of NMFA «Microstimulin» and the number of microorganisms in the products of slaughtering has been proved.

Influence of feed additive with Betulin on metabolism and productivity of broiler chickens

2021 ◽  
pp. 3-18
Author(s):  
S. A. Shpуnova ◽  
O. A. Yadrishchenskaya ◽  
T. V. Selina ◽  
E. A. Basova
Keyword(s):  
Broiler Chickens ◽  
Practical Importance ◽  
Live Weight ◽  
Biologically Active ◽  
Feed Consumption ◽  
Feed Additive ◽  
Control Group ◽  
Meat Production ◽  
Birch Bark ◽  
Experimental Group

In order to increase the meat productivity of broiler chickens, increase production volumes and reduce the prime-cost of poultry products, it is necessary not only to ensure balanced feeding and compliance with production technology but also to include in the poultry diet non-traditional additives, biologically active substances that contribute to the normalization of metabolic processes and improve the digestibility of feed nutrients. One of these dietary additives is Betulin extracted from birch bark, which has antioxidant and immunomodulatory properties. In this regard, the purpose of the research was to study the influence of compound feed with the introduction of 2 % feed additive based on a synthetic microspherical nanoporous carbon carrier containing biologically active Betulin in its composition on the productivity of broilers. The results of studies on broiler chickens using compound feed with 2 % feed additive have been presented in the article. It has been found that the feed consumption per 1 kg of live weight gain in poultry of the experimental group have been lower by 9,5 % due to better absorption of nutrients, the live weight of broiler chickens of the experimental group at the age of 42 days was 2625,6 g and it was higher than the control group by 3,68 %. The digestibility of crude protein and fat reached 92,73 and 88,03 %, which exceeded the control group by 1,03 and 3,03 abs.%, respectively. It has been found that the use of compound feed with 2 % of the feed additive with the biologically active substance is of practical importance in connection with the increase in the productivity of broilers and the level of profitability of meat production by 8,2 abs.%.

COMPLEX FEED ADDITIVE TO NORMALIZE METABOLIC PROCESSES OF COWS

2021 ◽  
pp. 30-33
Author(s):  
Н.В. БОГОЛЮБОВА ◽  
Р.А. РЫКОВ
Keyword(s):  
The Body ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Feed Additive ◽  
Feed Supplement ◽  
Metabolic Processes ◽  
Activity Of Enzymes ◽  
Experimental Group ◽  
Active Substances ◽  
Alt Activity

Изучен способ нормализации обменных процессов и повышения продуктивности молочных коров при использовании комплекса биологически активных веществ. Эксперимент проведен на коровах голштинской породы (n=12) с продуктивностью за предыдущую лактацию 9000 кг молока. За 20 дней до отела и в течение 65 дней после коровы опытной группы получали комплексную кормовую добавку (ККД), включающую минерал шунгит, холин в «защищенной» форме, пробиотик «Целлобактерин+» и жмых льняной, в количестве 200 г на голову в сутки в смеси с концентратами. Для изучения обменных процессов в организме коров (n=5) в конце опыта отобраны пробы крови и определены основные биохимические показатели. Для оценки продуктивных качеств проводили контрольные дойки. Отмечено повышение в крови коров опытной группы концентрации общего белка на 2,8%, глюкозы — на 26,8% (P≤0,01), фосфолипидов — на 15,2% (P≤0,05), снижение мочевины на 23,6% (P≤0,05), билирубина — на 23,8% (P≤0,01), активности АЛТ на 13,11 (P≤0,05), АСТ — на 14,6% (P≤0,05). Использование ККД позволило снизить и привести в норму активность ферментов: лактатдегидрогеназы (ЛДГ), гаммаглутаминтрансферазы (ГГТ), креатинкиназы. Повышение интенсивности обменных процессов и нормализация показателей, характеризующих работу печени с использованием в питании комплекса биологически активных веществ ферментно-пробиотического, антиоксидантного и липотропно-гепатопротекторного действия, привело к повышению молочной продуктивности. A method of normalizing metabolic processes and increasing the productivity of dairy cows using a complex of biologically active substances has been studied. The experiment was carried out on Holstein cows (n = 12) with a productivity for the previous lactation of 9000 kg of milk. 20 days before calving and within 65 days after the cows of the experimental group received a complex feed supplement (CFS), including the mineral shungite, choline in a "protected" form, the probiotic "Cellobacterin +" and flaxseed cake, in an amount of 200 g per head per day mixed with concentrates. To study metabolic processes in the body of cows (n = 5), at the end of the experiment, blood samples were taken and the main biochemical parameters were determined. To assess the productive qualities, control milking was carried out. An increase in the blood of cows of the experimental group was noted in the concentration of total protein by 2.8%, glucose - by 26.8% (P≤0.01), phospholipids — by 15.2% (P≤0.05), a decrease in urea by 23,6% (P≤0.05), bilirubin - by 23.8% (P≤0.01), ALT activity by 13.11 (P≤0.05), AST — by 14.6% (P≤ 0.05). The use of CFS made it possible to reduce and normalize the activity of enzymes: lactate dehydrogenase (LDH), gamma glutamine transferase (GGT), creatine kinase. An increase in the intensity of metabolic processes and the normalization of indicators characterizing the work of the liver with the use of a complex of biologically active substances of enzymatic-probiotic, antioxidant and lipotropic-hepatoprotective action in the diet led to an increase in milk productivity.

scholarly journals Features of protein metabolism in the body of broiler chickens when using the feed additive Peak-Antistress in the diet

2020 ◽  
pp. 103-110
Author(s):  
A. V. Mitfakhutdinov ◽  
E. R. Saifulmuliukov
Keyword(s):  
Broiler Chickens ◽  
Protein Composition ◽  
Feed Additives ◽  
The Body ◽  
Feed Additive ◽  
Control Group ◽  
Protein Accumulation ◽  
White Meat ◽  
Scientific Publications ◽  
Experimental Group

Scientific publications describe the positive effect of feed additives and pharmacological complexes on metabolic processes in the body of broiler chickens, in particular, on the protein composition of the blood, meat productivity and protein accumulation in meat. The Peak-Antistress feed additive developed at the Department of Morphology, Physiology and Pharmacology of the South Ural State Agrarian University allows to reduce the technological load on the poultry body by stimulating the general metabolism and complex antioxidant action. Experiments on the use of the feed additive Peak-Antistress were carried out on broiler chickens of the final hybrid of the Arbor Acres cross in an industrial-type poultry farm with floor technology. The poultry was divided into three groups of 6,000 chickens each and kept in one workshop in separate sections. The control group received the main diet, the 1st experimental group received the main diet and feed additive at a dose of 1269 g / t of feed 5 days before slaughter, the 2nd experimental group received the main diet and feed additive at a dose of 1693 g / t of feed 5 days before slaughter. Slaughter of broiler chickens was carried out according to the technological instructions of the enterprise on the 38th day. Against the background of the use of the feed additive in the experimental groups of broiler chickens, stimulation of protein metabolism was observed, as evidenced by an increase in total protein in the blood by 7.9–20.1%, an increase in the level of protein in white meat by 0.1–0.3%, and a tendency to an increase in amino acids in white meat protein by 0.2–12.3%.

scholarly journals Efficiency of energy feed supplement Cobiotic Energy in diets for cows

2019 ◽  
pp. 81-92
Author(s):  
V. Radchicov ◽  
V. Tzai ◽  
A. Kot ◽  
T. Sapsaleva ◽  
G. Besarab ◽  
...  
Keyword(s):  
Dry Matter ◽  
Feed Additives ◽  
Metabolizable Energy ◽  
Feed Additive ◽  
Control Group ◽  
Feed Supplement ◽  
Protein Ratio ◽  
Supplement Use ◽  
Group Ii ◽  
Experimental Group

The influence of feeding high-performance cows of the energy feed additive Cobiotic Energy on the physiological state and productivity have been studied. Experiments have been carried out on with groups of dairy cows with 10 animals each during 55 days. The diet of cows of the first control group consisted of haylage of cereal and leguminous plants, corn silage, molasses, cereal hay, and feed of own production. 0.3 kg of feed additive has been introduced into the diet of animals of the experimental group II 15 days before calving, in the first 10 days after calving, as compensation for energy losses, 0.75 kg 3 times a day. In the next 30 days of the milking season with the ration of animals of the experimental group II, 0.25 kg of Cobiotic Energy has been fed, sprinkling it on top of the feed mixture. It has been established that the use of feed additives in the diets of cows has a positive effect on feed intake, physiological condition and animal productivity. It was determined that concentration of metabolizable energy in 1 kg of dry matter of the diet made 9–16– 9.2 MJ. The diet contained 12.8–13.0 % of crude protein per 1 kg of dry matter, the concentration of fiber in dry matter made 22.3– 22.0 %. The sugar-protein ratio made 1.04:1; 1.0. In terms of energy nutrition, the diet of the experimental group due to the inclusion of the feed supplement was 6 MJ higher, however, it slightly affected concentration of metabolizable energy of the diet. The energy-protein ratio corresponded to 0.2– 0.21. The nitrogen balance in the rumen was positive in all the animals and was at the level of 0.13–0.4 g. Feeding animals with supplement in the diet in the last 15 days of the dry period, during the first 10 days after calving and the next 30 days of the milking period promoted activation of protein biosynthesis and energy metabolism, as evidenced by an increase in serum total protein and its albumin fraction and ultimately increased animal productivity. The studied supplement use in the diets for cows makes it possible to increase performance of cows by 8.0 %, fat content by 0.1 %, protein by 0.24 %, lactose by 0.11 %, reduce the cost of feed for obtaining natural milk by 6.1%. Key words: feed supplement Cobiotic Energy, cows, diets, blood, milk yield, feed costs, milk quality.

scholarly journals Organic Poultry Farming and Meat Productivity Stimulation of Broiler Chickens

2020 ◽  
pp. 57-60
Author(s):  
Е.А. Капитонова ◽  
П.В. Арефьев ◽  
Л.П. Мищенко
Keyword(s):  
Organic Acids ◽  
Broiler Chickens ◽  
Research Work ◽  
Live Weight ◽  
Poultry Meat ◽  
Feed Additive ◽  
Veterinary Drug ◽  
Poultry House ◽  
Meat Productivity ◽  
Experimental Group

Цель научно-исследовательской работы – стимуляция продуктивности цыплят-бройлеров кросса «Росс-308» органическими кислотами без снижения питательности рациона птицы. Научно-производственный опыт проводился в условиях ОАО «Витебская бройлерная птицефабрика» Витебской области. Цыплятам-бройлерам кросса «Росс-308» в систему поения, через дозатор ветеринарных препаратов, с водой вводилась жидкая кормовая добавка на основе фульвокислоты (концентрация – 1 г/л ДВ). Исследования показали, что использование органических кислот при выращивании молодняка птицы способствовало активизации неспецифического иммунитета, увеличению сохранности поголовья и повышению мясных качеств цыплят-бройлеров кросса «Росс-308». Так, наблюдалось увеличение средней живой массы птицы к убойному возрасту в опытной группе по сравнению с контролем на 0,8%. От опытной группы птицы было получено на 0,6% (+416 шт.) товарных тушек больше, чем от птицы, выращиваемой в контрольном птичнике. В опытном птичнике было получено тушек I сорта на 0,7 процентных пунктов больше, чем в контрольном птичнике. Соответственно, тушек II сорта было в опытном птичнике получено меньше, по сравнению с контролем. Получение несортовых тушек отмечено не было, так как согласно технологическим правилам предприятия некондиционная птица еженедельно выбраковывается. В совокупности все достигнутые результаты позволили получить весомое увеличение валового производства мяса птицы и дополнительную прибыль для предприятия. The purpose of the research work is to stimulate the productivity of Ross-308 cross broiler chickens with organic acids without reducing the nutritional value of the poultry diet. Scientific and production trial was carried out in the conditions of OAO Vitebsk Broiler Poultry Farm in the Vitebsk Region. Broiler chickens of the Ross-308 cross were introduced into the drinking system through a veterinary drug dispenser, with water a liquid feed additive based on fulvic acid (concentration – 1 g/l AD). Researches have shown that the use of organic acids in the growing of young poultry contributed to the activation of non-specific immunity, an increase in the safety of the livestock and an increase in the meat qualities of Ross-308 cross broiler chickens. Thus, there was an increase in the average live weight gain of poultry by slaughter age in the experimental group compared with the control by 0.8%. From the experimental group of poultry 0.6% (+416 pcs.) more marketable birds were received than from poultry grown in the control poultry house. In the experimental poultry house, birds of 1st class were obtained 0.7 percentage points more than in the control poultry house. Accordingly, birds of 2ndclass were obtained in the experimental poultry house less than in the control. The receiving of grade-out birds was not noted, since according to the technological rules of the enterprise, an off-grade poultry is rejected weekly. For a total all the results achieved made it possible to get a significant increase in the gross production of poultry meat and additional profit for the enterprise.

scholarly journals Administration of Clinoptilolite to Broiler Chickens During Growth and Its Effect on the Growth Rate and Bone Metabolism Indicators

2008 ◽  
Vol 77 (2) ◽  
pp. 199-207 ◽  
Author(s):  
E. Straková ◽  
R. Pospíšil ◽  
P. Suchý ◽  
L. Steinhauser ◽  
I. Herzig
Keyword(s):  
Growth Rate ◽  
Bone Metabolism ◽  
Broiler Chickens ◽  
Dry Matter ◽  
Live Weight ◽  
Ash Content ◽  
Feed Additive ◽  
Feed Mixture ◽  
Calcium Phosphorus ◽  
Experimental Group

The growth rate and bone metabolism indicators were monitored in broiler chickens receiving the feed supplemented with clinoptilolite. One-day-old broiler chickens ROSS 308 were divided into control (C) and experimental (E) groups with 100 males and 100 females per group. The chickens received the complete feed mixture BR1 from 1 to 10 days of age, followed by the feed mixture BR2 until the age of 30 days, and the feed mixture BR3 until the end of the experiment (40 days). The feed mixtures of the experimental group were supplemented with clinoptilolite (commercial additive ZeoFeed) at a level of 0.5% (BR1), 1.5% (BR2) and 2.5% (BR3), replacing the corresponding portion of wheat. Feed mixtures and drinking water were provided ad libitum. The live weight of broiler chickens in both the control and experimental group increased steadily during the experiment. At the end of the experiment, live weights of experimental females (2,416 g) and males (2,829 g) were higher than those of control females (2,345 g) and males (2,694 g) by 3% and 5%, respectively. Significant differences in the live weight between groups were found from the age of 30 days (P ⪬ 0.05 and P ⪬ 0.01). At the age of 40 days, the chickens were slaughtered and the femur and tibiotarsus of the right leg were analysed for the content of dry matter, ash, calcium, phosphorus and magnesium. The ash content in dry matter ranged from 53.0 to 54.1% in group C and from 51.7 to 53.2% in group E. The Ca and P contents in dry matter in group E were lower than those in group C, except for Ca and P in the male tibiotarsus. In both groups, regardless of sex, the ash content was higher in the tibiotarsus than in the femur. Since fat levels in bones of the experimental group were increased (females by 19.5% in the femur and 21.3% in the tibiotarsus; males by 22.0% in the femur and 26.3% in the tibiotarsus), which could affect the values obtained, ash, calcium, phosphorus and magnesium were determined in the fat-free dry matter. The trends found in the originally determined dry matter were maintained, i.e. lower levels of ash, Ca, and P in the experimental group, except for male Ca in both kinds of bones. The corresponding levels in the fat-free dry matter were relatively higher as compared to the original dry matter. The results of the study showed that up to 2.5% of clinoptilolite in the diet had no adverse effect on performance and bone metabolism indicators of growing broiler chickens. Thus, it can be used as a suitable feed additive to broiler diets due to its positive effect on nutrient utilization, mechanism of digestion and pollutant elimination in connection with food safety issues.

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