scholarly journals PSXII-42 The lactational response in dairy cows to supplementation of calcium gluconate embedded in a fat matrix

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 430-430
Author(s):  
John Doelman ◽  
Leslie McKnight ◽  
Pieter Winia ◽  
Gera Uittenbogaard ◽  
Michelle Carson ◽  
...  

Abstract Functional nutrients that facilitate gastrointestinal health may serve as an alternative to the use of antimicrobials in animal production systems. Gluconic acid has been used as a prebiotic health promoter in non-ruminant applications. It is poorly absorbed in the small intestine and is fermented to butyrate in the lower gastrointestinal tract. The gut health benefits of butyrate have been demonstrated in both non-ruminant and ruminant models, but effects of post-ruminal gluconic acid supplementation are not well described. The objective of this study was to determine the effects of calcium gluconate embedded in a fat matrix (CGFM; 40% CaGlu) on dry matter intake, blood metabolites, milk production and fecal short chain fatty acid (SCFA) production in early lactation dairy cows (21 ± 2 DIM) fed to 100% of energy and protein requirements. The experiment was a 3 x 3 replicated Latin square with 13 replicates (3 cows/replicate), 3 experimental periods (each 28 d) and 3 dietary treatments of control (0 g/d), 16 g/d and 25 g/d of CGFM. There was no treatment effect on DMI and milk yield. The relationship between dose of CGFM (i.e. 0, 16, 26 g/d) and milk fat and protein yield, energy corrected milk was significantly quadratic (P < 0.05) while feed efficiency tended to be quadratic (P = 0.08). The greatest response was in milk fat yield, which increased 90 g/d over control at 16 g/d of CGFM. No significant changes in blood metabolite concentrations or fecal SCFA concentrations were observed between treatments, although there was a numerical increase in non-esterified fatty acid concentration in response to the 16 g/d CGFM dose (P = 0.16). The response to supplementation of fat-embedded calcium gluconate appears to alter energy partitioning in the lactating dairy cow, as demonstrated with subtle changes in circulating lipid and increased fat yield.

2019 ◽  
Vol 102 (2) ◽  
pp. 1274-1280 ◽  
Author(s):  
John Doelman ◽  
Leslie L. McKnight ◽  
Michelle Carson ◽  
Kelly Nichols ◽  
Douglas F. Waterman ◽  
...  

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1465
Author(s):  
Jesus A. C. Osorio ◽  
João L. P. Daniel ◽  
Jakeline F. Cabral ◽  
Kleves V. Almeida ◽  
Karoline L. Guimarães ◽  
...  

This study aimed to evaluate the effects of annatto seeds, linseed oil and their combination on DMI, apparent total tract digestibility, antioxidant capacity and milk composition of dairy cows. Four lactating Holstein cows (120 ± 43 days in milk; 15.98 ± 2.02 kg of milk/day, mean ± SD) were allocated in a 4 × 4 Latin square with a 2 × 2 factorial arrangement (with or without annatto seeds at 15 g/kg of dry matter (DM); with or without linseed oil at 30 g/kg of DM) and provided four different diets: control (no annatto seeds or linseed oil); annatto seeds (15 g/kg of DM); linseed oil (30 g/kg of DM); and a combination of both annatto seeds and linseed oil. Annatto seeds reduced DM intake, and milk yield, protein and lactose, but increased content of fat, total solids and short chain fatty acid, with no effect on total antioxidant capacity of milk. Linseed oil supplementation decreased medium chain fatty acid proportion and n-6/n-3 ratio, conversely it increased long chain fatty acids and n-3 fatty acid content of milk, ether extract intake and total-tract digestibility. Thus, linseed oil supplementation in dairy cow diets improved the milk FA profile but decreased milk fat concentration, whereas annatto seeds did not influence antioxidant capacity and depressed feed intake and milk yield.


2020 ◽  
Vol 124 (4) ◽  
pp. 386-395
Author(s):  
D. E. Rico ◽  
J. E. Parales ◽  
B. A. Corl ◽  
A. Lengi ◽  
P. Y. Chouinard ◽  
...  

AbstractThe aim of the present study was to compare the effects of post-ruminally infused fat supplements, varying in fatty acid (FA) chain length, on animal performance, metabolism and milk FA. Eleven multiparous Holstein dairy cows were used in a replicated incomplete 3 × 3 Latin square design with 7-d periods, separated by 7-d washouts. Treatments were administered as abomasal infusions of enrichments providing 280 g/d of FA: (1) palmitic acid (98·4 % 16 : 0; PA), (2) caprylic and capric acids (56·2 % 8 : 0, 43·8 % 10 : 0; medium-chain TAG (MCT)) and (3) stearic acid (99·0 % 18 : 0; SA). Relative to PA, SA decreased the efficiency of fat-corrected milk production, which was associated with a tendency for higher DM intake and lower FA absorption with SA, whereas MCT was not different from PA for these variables. Milk fat concentration and yield were increased by PA relative to SA, but only fat yield tended to be greater relative to MCT. Relative to PA, MCT increased milk fat concentration of FA < 16 C, whereas SA increased FA > 16 C. Expression of mammary stearoyl-coA desaturase 1 was lower with SA than with PA. Relative to PA, liver expression of adenosine monophosphate-activated protein kinase-1 and pyruvate kinase was increased with MCT, whereas expression of these genes tended to be increased by SA. The mechanism of increased fat secretion with PA does not seem to be related to a modulation of the expression of lipogenesis-related genes, but rather to increased substrate availability as reflected by milk FA profile.


2013 ◽  
Vol 111 (6) ◽  
pp. 1011-1020 ◽  
Author(s):  
Marie-France Palin ◽  
Cristiano Côrtes ◽  
Chaouki Benchaar ◽  
Pierre Lacasse ◽  
Hélène V. Petit

In the present study, the effect of flax hulls with or without flax oil bypassing the rumen on the expression of lipogenic genes in the mammary tissue of dairy cows was investigated. A total of eight dairy cows were used in a replicated 4 × 4 Latin square design. There were four periods of 21 d each and four treatments: control diet with no flax hulls (CONT); diet with 9·88 % flax hulls in the DM (HULL); control diet with 500 g flax oil/d infused in the abomasum (COFO); diet with 9·88 % flax hulls in the DM and 500 g flax oil/d infused in the abomasum (HUFO). A higher mRNA abundance of sterol regulatory element binding transcription factor, fatty acid (FA) synthase, lipoprotein lipase (LPL), PPARγ1, stearoyl-CoA desaturase (SCD) and acetyl-coenzyme A carboxylase-α was observed in cows fed HULL than in those fed CONT, and HUFO had the opposite effect. Compared with CONT, COFO and HUFO lowered the mRNA abundance of SCD, which may explain the lower proportions of MUFA in milk fat with flax oil infusion. The mRNA abundance of LPL in mammary tissue and proportions of long-chain FA in milk fat were higher in cows fed COFO than in those fed CONT. The highest proportions of trans FA were observed when cows were fed HULL. The present study demonstrates that flax hulls with or without flax oil infusion in the abomasum can affect the expression of lipogenic genes in the mammary tissue of dairy cows, which may contribute to the improvement of milk FA profile.


2014 ◽  
Vol 81 (2) ◽  
pp. 156-163 ◽  
Author(s):  
Ghazal Akbaridoust ◽  
Tim Plozza ◽  
Victor C Trenerry ◽  
William J Wales ◽  
Martin J Auldist ◽  
...  

This study investigated the effects of different strategies for feeding supplements to grazing dairy cows on the proportions of fatty acids in milk. Two hundred and sixteen cows were fed supplementary grain and forage according to one of 3 different strategies; (1) Control: cows grazed perennial ryegrass pasture (14 kg dry matter/d) supplemented with milled barley grain fed in the milking parlour and pasture silage offered in the paddock; (2) Partial mixed ration 1 (PMR1): same pasture allotment and supplement as Control strategy, but the supplements presented as a mixed ration after each milking in feedpad, and; (3) Partial mixed ration 2 (PMR2): same pasture allotment, supplemented with a mixed ration of milled barley grain, alfalfa hay, corn silage and crushed corn grain fed in a feedpad. Within each strategy, cows were assigned to receive either 6, 8, 10 or 12 kg dry matter supplement/cow per d. Milk fatty acid proportions from cows fed Control and PMR1 strategies were similar and different from those fed PMR2, particularly at 10 to 12 kg dry matter supplement/cow per d. The reduction in milk fat yield and concentration in cows fed high amounts of supplement as Control and PMR1 was coincident with 4 × increase in 10t-18:1 proportion. The composition of the partial mixed ration (PMR) and the amount offered affected milk fatty acid proportions and milk fat content, however, the method of supplementation did not.


Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 919
Author(s):  
Verónica M. Merino ◽  
Lorena Leichtle ◽  
Oscar A. Balocchi ◽  
Francisco Lanuza ◽  
Julián Parga ◽  
...  

The aim was to determine the effect of the herbage allowance (HA) and supplement type (ST) on dry matter intake (DMI), milk production and composition, grazing behavior, rumen function, and blood metabolites of grazing dairy cows in the spring season. Experiment I: 64 Holstein Friesian dairy cows were distributed in a factorial design that tested two levels of daily HA (20 and 30 kg of dry matter (DM) per cow) and two ST (high moisture maize (HMM) and cracked wheat (CW)) distributed in two daily rations (3.5 kg DM/cow/day). Experiment II: four mid-lactation rumen cannulated cows, supplemented with either HMM or CW and managed with the two HAs, were distributed in a Latin square design of 4 × 4, for four 14-d periods to assess ruminal fermentation parameters. HA had no effect on milk production (averaging 23.6 kg/day) or milk fat and protein production (823 g/day and 800 g/day, respectively). Cows supplemented with CW had greater protein concentration (+1.2 g/kg). Herbage DMI averaged 14.17 kg DM/cow.day and total DMI averaged 17.67 kg DM/cow.day and did not differ between treatments. Grazing behavior activities (grazing, rumination, and idling times) and body condition score (BCS) were not affected by HA or ST. Milk and plasma urea concentration increased under the high HA (+0.68 mmol/L and +0.90 mmol/L, respectively). Cows supplemented with HMM had lower milk and plasma urea concentrations (0.72 mmol/L and 0.76 mmol/L less, respectively) and tended (p = 0.054) to have higher plasma β-hydroxybutyrate. Ruminal parameters did not differ between treatments.


Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3122
Author(s):  
Jalil Ghassemi Nejad ◽  
Bae-Hun Lee ◽  
Ji-Yung Kim ◽  
Kyung-Il Sung ◽  
Hong-Gu Lee

The effects of grazing lactating cows in mountainous areas for 12 and 24 h compared with the confined indoor system were evaluated by examining the overall milk fatty acid and cortisol. Twenty-one dairy cows were allocated to three treatment groups: (1) control (confined management system in a free-stall barn; TMR based), (2) grazing for 12 h (12hG; TMR plus grazing pasture), and (3) grazing for 24 h (24hG; pasture-based feeding system). Dry matter intake was higher in the control and 12hG groups than in the 24hG group. The yields of total milk and the 3.5% fat-corrected milk were the lowest in the 24hG group. Milk fat was the highest in the 24hG group and higher in 12hG compared with the control group. Milk protein and lactose levels were the highest in the 12hG group. The highest somatic cell count was observed in the 24hG group. The saturated fatty acid levels were higher in the control group compared with the 12hG and 24hG groups. There was no difference in overall mono-unsaturated fatty acids between 12hG and 24hG groups. Poly-unsaturated fatty acids were higher in the 12hG group compared with the control and 24hG groups. There was no difference in omega-6 (ω-6) fatty acids among the groups, and omega-3 fatty acids were higher in the 12hG group than in the control group. Milk cortisol was the highest in the 24hG group and higher in the control group compared with the 12hG group. Taken together, grazing for 12 h is advisable for farms that have access to mountainous areas to improve the milk fatty acid profile and decrease the stress levels in high-yielding Holstein lactating cows.


1980 ◽  
Vol 31 (4) ◽  
pp. 368-374 ◽  
Author(s):  
William Banks ◽  
John L. Clapperton ◽  
Morag E. Kelly ◽  
Agnes G. Wilson ◽  
Robert J. M. Crawford

Animals ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 57 ◽  
Author(s):  
Frank Dunshea ◽  
Kehinde Oluboyede ◽  
Kristy DiGiacomo ◽  
Brian Leury ◽  
Jeremy Cottrell

Betaine is an organic osmolyte sourced from sugar beet that accumulates in plant cells undergoing osmotic stress. Since the accumulation of betaine lowers the energy requirements of animals and, therefore, metabolic heat production, the aim of this experiment was to investigate if betaine supplementation improved milk yield in grazing dairy cows in summer. One hundred and eighteen Friesian × Holstein cows were paired on days in milk and, within each pair, randomly allocated to a containing treatment of either 0 or 2 g/kg natural betaine in their concentrate ration for approximately 3 weeks during February/March 2015 (summer in Australia). The mean maximum February temperature was 30 °C. Cows were allocated approximately 14 kg dry matter pasture and 7.5 kg of concentrate pellets (fed in the milking shed) per cow per day and were milked through an automatic milking system three times per day. Betaine supplementation increased average daily milk yield by over 6% (22.0 vs. 23.4 kg/day, p < 0.001) with the response increasing as the study progressed as indicated by the interaction (p < 0.001) between betaine and day. Milk fat % (p = 0.87), milk protein % (p = 0.90), and milk somatic cell count (p = 0.81) were unchanged by dietary betaine. However, betaine supplementation increased milk protein yield (677 vs. 719 g/day, p < 0.001) and fat yield (874 vs. 922 g/day, p < 0.001) with responses again being more pronounced as the study progressed. In conclusion, dietary betaine supplementation increased milk and component yield during summer in grazing dairy cows.


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