scholarly journals 1727. Sustained Antimicrobial Activity of a Novel Disinfectant Against Healthcare Pathogens

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S55-S55 ◽  
Author(s):  
William Rutala ◽  
Maria Gergen ◽  
Emily Sickbert-Bennett ◽  
Deverick J Anderson ◽  
David Weber
Keyword(s):  
Hydrogen Peroxide ◽  
Antimicrobial Activity ◽  
Stainless Steel ◽  
The Novel ◽  
Log10 Reduction ◽  
Final Time ◽  
E Coli ◽  
Environmental Surfaces ◽  
The Mean

Abstract Background Environmental contamination plays an important role in the transmission of MRSA, VRE, and C. difficile. Suboptimal compliance with hand hygiene or inappropriate glove use can result in indirect transfer of these pathogens to patients. This study evaluates a novel disinfectant that claims to kill microbes on surfaces for ≥24 hours. Methods We investigated the persistent antimicrobial activity of a novel disinfectant using an EPA protocol for sustained disinfecting activity. In brief, surfaces are inoculated, treated with the novel disinfectant, allowed to dry, and then abraded using a standardized abrasion machine under multiple alternating wet and dry wipe conditions (N = 12) interspersed with 6 re-inoculations. After 24 hours, the surface was re-inoculated a final time and ability of the disinfectant to kill ≥99.9% of 9 test microbes within 5 minutes was measured on 3 test surfaces (glass, formica, and stainless steel). Results The novel disinfectant demonstrated a 3–5 log10 reduction in 5 minutes when testing S. aureus, VRE, C. auris, CRE E. coli and antibiotic-sensitive strains of E. coli, and Enterobacter sp. (table). The disinfectant demonstrated lower killing for CRE isolates of Enterobacter sp. and K. pneumoniae, and for antibiotic-sensitive K. pneumoniae (~2 log10 reduction in 5 minutes). When the novel disinfectant was compared with 3 other commonly used disinfectants using the same methodology with S. aureus, the mean log10 reductions were: 4.4 (novel disinfectant); 0.9 (quat-alcohol); 0.2 (improved hydrogen peroxide); and 0.1 (chlorine). Conclusion Persistent disinfectants may reduce or eliminate the problem of recontamination and minimize the role of environmental surfaces in transmission of healthcare pathogens. Disclosures W. Rutala, PDI: Consultant and Speaker’s Bureau, Consulting fee and Speaker honorarium. D. Weber, PDI: Consultant, Consulting fee

scholarly journals Distribution of Lactoferrin Is Related with Dynamics of Neutrophils in Bacterial Infected Mice Intestine

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1496 ◽  
Author(s):  
Li Liang ◽  
Zhen-Jie Wang ◽  
Guang Ye ◽  
Xue-You Tang ◽  
Yuan-Yuan Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Mucosal Immunity ◽  
Mrna Levels ◽  
Iron Binding ◽  
E Coli ◽  
New Knowledge ◽  
Activated Neutrophils ◽  
Binding Glycoprotein

Lactoferrin (Lf) is a conserved iron-binding glycoprotein with antimicrobial activity, which is present in secretions that recover mucosal sites regarded as portals of invaded pathogens. Although numerous studies have focused on exogenous Lf, little is known about its expression of endogenous Lf upon bacterial infection. In this study, we investigated the distribution of Lf in mice intestine during Escherichia coli (E. coli) K88 infection. PCR and immunohistology staining showed that mRNA levels of Lf significantly increased in duodenum, ileum and colon, but extremely decreased in jejunum at 8 h and 24 h after infection. Meanwhile, endogenous Lf was mostly located in the lamina propria of intestine villi, while Lf receptor (LfR) was in the crypts. It suggested that endogenous Lf-LfR interaction might not be implicated in the antibacterial process. In addition, it was interesting to find that the infiltration of neutrophils into intestine tissues was changed similarly to Lf expression. It indicated that the variations of Lf expression were rather due to an equilibrium between the recruitment of neutrophils and degranulation of activated neutrophils. Thus, this new knowledge will pave the way to a more effective understanding of the role of Lf in intestinal mucosal immunity.

scholarly journals Key role of hydrogen peroxide in antimicrobial activity of spring,Honeydew maquisand chestnut grove Corsican honeys onPseudomonas aeruginosaDNA

2018 ◽  
Vol 66 (5) ◽  
pp. 427-433 ◽  
Author(s):  
J.-P. Poli ◽  
E. Guinoiseau ◽  
A. Luciani ◽  
Y. Yang ◽  
M.-J. Battesti ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antimicrobial Activity

scholarly journals The role of an innovative disinfection system based on silver and hydrogen peroxide in infection prevention

2021 ◽  
Vol 343 ◽  
pp. 10003
Author(s):  
Martin Heinisch ◽  
Laura Heinisch ◽  
Dan Miricescu
Keyword(s):  
Hydrogen Peroxide ◽  
Genetic Information ◽  
Infection Prevention ◽  
Surface Proteins ◽  
Transmission Route ◽  
The Novel ◽  
Disinfection Methods ◽  
Virus Integration ◽  
Virus Spreading

Due to the current pandemic situation caused by SARS-CoV-2 the need of effective precautionary methods is increasing. Besides the transmission of this virus by aerosols induced to air, it is assumed that the transmission route of SARS-CoV-2 is mainly by contaminated surfaces. It has been demonstrated that viruses can contaminate dry surfaces and can be further transmitted to the host even after extended time. The amount of disinfection and hygiene systems has increased drastically over the recent year. Although, the conventional disinfection method via spraying and wiping is labour intensive and efficacy is dependent on the application. Aim of this study was to improve conventional disinfection methods. This new disinfection system based on hydrogen peroxide and silver nanoparticles displays a quick and effective alternative. The composition which was proposed in this study shows unique features in terms of application, health risk and effectivity. The novel application by vaporization helps to disinfect the environment and even the air to reduce virus spreading. New disinfection formulation shows efficacy on the surface proteins and genetic information of the virus. Integration of the effective disinfection method shown in this study in the current precaution measurements will help to reduce the spread of SARS-CoV-2.

scholarly journals Hierarchical Porous Polyamide 6 by Solution Foaming: Synthesis, Characterization and Properties

Polymers ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1310 ◽  
Author(s):  
Liang Wang ◽  
Yu-Ke Wu ◽  
Fang-Fang Ai ◽  
Jie Fan ◽  
Zhao-Peng Xia ◽  
...  
Keyword(s):  
Large Scale ◽  
Polyamide 6 ◽  
Solution Concentration ◽  
The Novel ◽  
Foaming Agent ◽  
Hierarchical Porous ◽  
Wide Range ◽  
The Mean ◽  
Novel Strategy

Porous polym er materials have received great interest in both academic and industrial fields due to their wide range of applications. In this work, a porous polyamide 6 (PA6) material was prepared by a facile solution foaming strategy. In this approach, a sodium carbonate (SC) aqueous solution acted as the foaming agent that reacted with formic acid (FA), generating CO2 and causing phase separation of polyamide (PA). The influence of the PA/FA solution concentration and Na2CO3 concentration on the microstructures and physical properties of prepared PA foams were investigated, respectively. PA foams showed a hierarchical porous structure along the foaming direction. The mean pore dimension ranged from hundreds of nanometers to several microns. Low amounts of sodium salt generated from a neutralization reaction played an important role of heterogeneous nucleation, which increased the crystalline degree of PA foams. The porous PA materials exhibited low thermal conductivity, high crystallinity and good mechanical properties. The novel strategy in this work could produce PA foams on a large scale for potential engineering applications.

scholarly journals The Role of theExo-XisRegion in Oxidative Stress-Mediated Induction of Shiga Toxin-Converting Prophages

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Katarzyna Licznerska ◽  
Aleksandra Dydecka ◽  
Sylwia Bloch ◽  
Gracja Topka ◽  
Bożena Nejman-Faleńczyk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Shiga Toxin ◽  
Regulatory Genes ◽  
Prophage Induction ◽  
Dramatic Decrease ◽  
E Coli ◽  
Genetic Elements ◽  
Efficient Expression

Previous studies indicated that these genetic elements could be involved in the regulation of lysogenization and prophage induction processes. The effects were dramatic in Shiga toxin-converting phageΦ24Bafter treatment with oxidative stress-inducing agent, hydrogen peroxide, while they were less pronounced in bacteriophageλand in both phages irradiated with UV. The hydrogen peroxide-caused prophage induction was found to be RecA-dependent. Importantly, in hydrogen peroxide-treatedE. colicells lysogenic for eitherλorΦ24B, deletion of theexo-xisregion resulted in a significant decrease in the levels of expression of the S.O.S. regulon genes. Moreover, under these conditions, a dramatic decrease in the levels of expression of phage genes crucial for lytic development (particularlyxis, exo, N, cro, O, Q, andR) could be observed inΦ24B-, but not inλ-bearing cells. We conclude that genes located in theexo-xisregion are necessary for efficient expression of both host S.O.S regulon in lysogenic bacteria and regulatory genes of Shiga toxin-converting bacteriophageΦ24B.

THERMODYNAMICS OF THE FERMI-LINEARIZED EXTENDED HUBBARD MODEL

1992 ◽  
Vol 06 (22) ◽  
pp. 3529-3541 ◽  
Author(s):  
ANDREA DANANI ◽  
ARIANNA MONTORSI ◽  
MARIO RASETTI
Keyword(s):  
Hubbard Model ◽  
Coulomb Interaction ◽  
Mean Field ◽  
Local Magnetic Moment ◽  
The Novel ◽  
Extended Hubbard Model ◽  
Site Cluster ◽  
The Mean ◽  
Two Phases

The fermi linearization scheme for a two-site cluster (dimer) is applied to the extended Hubbard model, including Coulomb interaction between nearest neighbour sites. The partition function and the order parameter, as well as the average double-occupation and local magnetic moment are evaluated. The theory is checked by extended numerical analysis, performed here in the homogeneous case, when pairing is zero, and deriving the explicit temperature and filling dependence of the above quantities. An interesting feature that emerges from such calculations is that the mean-field parameter characteristic of fermi-linearization is capable of describing two different phases, one of which is not present in the conventional Hubbard model. The order parameters corresponding to the two phases are explicitly derived. The novel phase can be ascribed to the n.n. Coulomb interaction, the role of whose strength V is analysed.

scholarly journals Study on gamma-irradiation degradation of chitosan swollen in H₂O₂ solution and its antimicrobial activity for E. coli

2014 ◽  
Vol 4 (4) ◽  
pp. 1-8
Author(s):  
Xuan Du Dang ◽  
Phuoc Phuc Bui ◽  
Thi Thuy Tran ◽  
Anh Quoc Le ◽  
Van Phu Dang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antimicrobial Activity ◽  
Gel Permeation Chromatography ◽  
Food Preservation ◽  
Hydrogen Peroxide Solution ◽  
Γ Irradiation ◽  
E Coli ◽  
Structure Changes ◽  
Radiation Degradation ◽  
Ft Ir

Degradation of chitosan in swollen state with hydrogen peroxide solution (5% w/v) by γ-irradiation was investigated. Molecular weight (Mw) of irradiated chitosan was determined by gel permeation chromatography (GPC). Fourier transform infrared (FT-IR) and ultraviolet-visible (UV-vis) spectrawere analyzed to study the structure changes of degraded chitosan. The results showed that the chitosan of low Mw ~30-45 kDawas efficiently prepared by γ-irradiation of chitosan swollen in hydrogen peroxide solution at low dose less than 20kGy. The main structure as well as the degree of deacetylation of the degraded chitosan was almost no significant change. Furthermore, the radiation degradation yield (Gs) was remarkably enhanced by the presence of H2O2. The obtained low Mw chitosan revealed high antimicrobial activity for E. colithat can be used for food preservation and other purposes as well.

Bactericidal Activity of 0.5% Bupivacaine With Preservatives on Microorganisms in the Human Skin Flora

1997 ◽  
Vol 22 (2) ◽  
pp. 178-184
Author(s):  
Tadakazu Sakuragi ◽  
Hiroyuki Ishino ◽  
Kenjiro Dan
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Body Temperature ◽  
Bactericidal Activity ◽  
Staphylococcus Epidermidis ◽  
Room Temperature ◽  
Physiological Saline ◽  
E Coli ◽  
Skin Flora ◽  
The Mean

Background and ObjectivesThe bactericidal activity of 0.5% bupivacaine with preservatives at body temperature and at room temperature is not known. We studied the bactericidal activity of 0.5% bupivacaine with 0.08% methyl para-oxybenzoate and 0.02% propyl para-aminobenzoate as preservatives and of the preservatives alone at 37°C and at room temperature on two strains of methicillin-resistant Staphylococcus aureus, two strains of methicillin-susceptible S. aureus, and one strain each of Staphylococcus epidermidis and Escherichia coli. Methods. The pathogen was exposed to 0.5% bupivacaine with preservatives or to the preservatives alone for 1, 3, 6, 12, and 24 hours at 37°C and at room temperature. The inocula from these suspensions were cultured for 48 hours at 37°C after the antimicrobial activity of bupivacaine was inactivated by 1:1,000 dilution with physiological saline.ResultsThe 1- through 12-hour exposures of four strains of S. aureus to 0.5% bupivacaine with preservatives at room temperature reduced the mean colony count by 24.2%, 49.2%, 71.3%, and 89.6%, respectively, and the exposure at 37°C reduced the count by 74.1%, 95.2%, 99.9%, and 99.8%, respectively. The differences for 1- through 12-hour exposures were significant (P < .001). The percentage kill in the strains of E. coli and S. epidermidis was significantly higher than that in the strains of S. aureus at all exposure times at room temperature (E. coli, P < .001; S. epidermidis, P < .0001) and at 1- and 3-hour exposures at 37°C (E. coli, P < .001; S. epidermidis, P < .0001). The bactericidal activity of the preservatives was markedly lower that that of 0.5% bupivacaine with preservatives (P < .0001).ConclusionsThe bactericidal activity of 0.5% bupivacaine with preservatives is stronger at body temperature than at room temperature; the bactericidal activity may be due, to a large extent, to bupivacaine rather than to the preservatives; and S. aureus is more resistant to the bactericidal activity of bupivacaine than are S. epidermidis and E. coli.

scholarly journals Comparison of Antibacterial Activity ofLactobacillus plantarumStrains Isolated from Two Different Kinds of Regional Cheeses from Poland: Oscypek and Korycinski Cheese

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Aleksandra Ołdak ◽  
Dorota Zielińska ◽  
Anna Rzepkowska ◽  
Danuta Kołożyn-Krajewska
Keyword(s):  
Hydrogen Peroxide ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Broad Spectrum ◽  
Antimicrobial Properties ◽  
Antagonistic Activity ◽  
Antimicrobial Compounds ◽  
E Coli ◽  
Unpasteurized Milk ◽  
Protective Cultures

Oscypek and korycinski are traditional Polish cheeses, exclusively produced in Tatra and in Podlasie region, respectively, produced from raw, unpasteurized milk. The 29Lactobacillus plantarumstrains were isolated on MRS agar from 12 cheese samples and used as a material for study. The main purpose of the work was to assess the antimicrobial properties and recognition of selected strains for the unique antagonistic activity and preservation role in food. It has been found that the highest antimicrobial activity was observed in the case ofL. monocytogenesstrains; however, the level of that activity was different depending on theLb. plantarumstrain. Strains from oscypek produced broad spectrum, and a few strains isolated from korycinski cheese produced a narrow spectrum of antimicrobial compounds, other than organic acids and hydrogen peroxide. Moreover, the antagonistic activity shown byLb. plantarumstrains is connected with the source from which a given strain was isolated. Strains isolated from oscypek cheese represented stronger activity againstL. monocytogenes, whereas strains isolated from korycinski cheese were more active againstE. coli. StrainsLb. plantarumOs13 and Kor14 could be considered as good candidates for protective cultures to extend durability of food products.

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