Clinical Pattern of Inflammatory bowel disease in central reigon, Saudi Arabia

Abstract Background The identification of semen stain is one of the most common human stains that can provide crucial information for crime scene reconstruction and forensic investigation. In sexual assault cases semen identification helps to prove victim’s allegations it also provides a material for DNA analysis that generate the genetic profile of the alleged suspect. The rapid Stain Identification of Human Semen (RSIDTM-Semen) bioassay is designed to detect specifically the presence of human semenogelin. Test development is completed within 10 minutes and can detect as little as 2.5 nL of human semen and it does not cross-react with other human or nonhuman tissues. The detection protocol can be completely integrated into the procedures for DNA extraction and analysis. Aim To assess the efficacy of RSIDTM – Semen strip test for the detection of human semen under some different variables (different fabrics, different time intervals and mixed with vaginal secretions) in order to be used in the routine forensic work of sexual assault cases and for further personal identification. Methodology: Semen samples were collected from four male participants. Vaginal specimens were collected from four female participants on cotton, linen or nylon-tipped swabs (2 swabs from each female). Each semen sample was divided into two portions; one used for semen only test group and the other mixed with vaginal secretions for the mixed test group. One of the fabrics tipped vaginal swab was mixed with semen for the mixed test group and the other used as a positive control group to test the sensitivity and specificity of the RSIDTM – Semen strip. The semen samples were deposited over different fabrics at the same time. All the samples were left to dry for 15 minutes at room temperature then extracted and analyzed according to the protocol designed for The Rapid Stain Identification Test (RSIDTM – Semen). The collected samples were studied as two test groups and one control group. Each of the previous groups, was categorized into 4 subgroups (a, b, c and d) according to the time interval of semen extraction (zero (on the spot), 2, 4, 6 and 10 days respectively). Results Semen could be identified in 100% of tested samples of the semen only group as well as of the combined semen and vagina group over cotton and linen fabrics at all the different tested time intervals. However, semen extracted from nylon fabric was identified in tested samples of the semen only group and of the combined semen and vagina group only at zero time only and couldn’t be identified at the rest of tested time intervals. Conclusion the current study evidenced that the new RSIDTM-semen kit is a reliable method for semen identification over different types of fabrics even in the presence of vaginal secretions. It also resists environmental factors up to 10 days. Background: Inflammatory bowel disease (IBD) is A chronic inflammatory disorder of the gastrointestinal tract (GIT). IBD comprises Crohn’s disease (CD) and Ulcerative Colitis (UC). Patients with CD and UC may present with variety of clinical presentations according to the activity of the disease. The treatment of IBD varies according to degree of activity. The demographics and clinical patterns of IBD have not been adequately studies in Saudi Arabia. Thus, the current study investigated the clinical presentation, course and outcome of IBD in a hospital in the Central region, Saudi Arabia. Methodology This prospective study included 92 patients from December 1, 2017, to August 31, 2018. The study constituted clinical history, screening through surveys that consisted of open ended and closed ended questions, and physical and laboratory assessment. Result There was no significant gender difference presents by male to female (M: F) 20:21 in UC and 23:28 in CD. The study showed that the most affected age group was the 4th decade in age group 31 to 40 years old represent that 56%. The most common presenting symptoms in active ulcerative colitis and Crohn’s disease were diarrhea, abdominal pain , weight loss, headache and fever. The study patients presented in different stages according to Montreal classification with the highest prevalence in Montreal stage two by 16.30% in UC and 22.82% in CD. Among patients with diarrhea, the vast majority due to infectious cause followed by UC the CD. Arthralgia’s, arthritis and ophthalmic manifestations were the most frequent extra-intestinal manifestations. Conclusion As progression of IBD in Saudi Arabia the health organization should research more in IBD and the cause of increase it’s incidence

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Rapid stain biomarker: A novel tool for forensic detection of semen (effect of time, different fabrics and vaginal secretions on its reliability)

QJM ◽

10.1093/qjmed/hcaa049 ◽

2020 ◽

Vol 113 (Supplement_1) ◽

Author(s):

A A Abdelrahman ◽

S A Mahmoud ◽

E A Attia

Keyword(s):

Sexual Assault ◽

Test Group ◽

Personal Identification ◽

The Other ◽

Control Group ◽

Time Interval ◽

Genetic Profile ◽

Human Semen ◽

Time Intervals ◽

Vaginal Secretions

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Do mouthwashes with and without bleaching agents degrade the force of elastomeric chains?

The Angle Orthodontist ◽

10.2319/081012-646.1 ◽

2013 ◽

Vol 83 (4) ◽

pp. 712-717 ◽

Cited By ~ 7

Author(s):

Matheus Melo Pithon ◽

Ailana Cardoso Rodrigues ◽

Érica Luiza Santana Moreira Sousa ◽

Lília Paula de Souza Santos ◽

Natália dos Santos Soares

Keyword(s):

Artificial Saliva ◽

Initial Period ◽

Test Group ◽

Experimental Period ◽

The Other ◽

Control Group ◽

Distilled Water ◽

Bleaching Agent ◽

Time Intervals ◽

Elastomeric Chains

ABSTRACT Objective: To evaluate, in vitro, the effect of mouthwashes with and without bleaching agents on the force of elastomeric chains. Materials and Methods: A total of 108 elastomeric chain specimens was divided into six groups (n = 18 in each group). Two test groups were exposed to two types of commonly used mouthwashes (Plax and Listerine), and two groups were exposed to mouthwashes containing bleaching agent (Plax Whitening and Listerine Whitening). Immersion in the solutions was performed twice a day for 60 seconds. One group of control specimens remained immersed in artificial saliva throughout the entire experimental period, and the other control specimens were exposed to distilled water. Force measurements were performed at six time intervals (initial, 1 day, 7 days, 14 days, 21 days, and 28 days). Results: No statistically significant differences were found between the groups in the initial period (P > .05). Statistically significant differences were found between the control group and the Plax, Plax Whitening, and Listerine groups at the time intervals of 7, 14, and 21 days. In the initial period, the force was statistically significantly higher than it was in any of the other experimental periods (P < .05). The control group with distilled water and the test group with Plax Whitening maintained the most force during the experimental period. Conclusion: The presence of bleaching agent has no influence on the force degradation of elastomeric chains.

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Use of Platelet-Rich Fibrin Associated with Xenograft in Critical Bone Defects: Histomorphometric Study in Rabbits

Symmetry ◽

10.3390/sym11101293 ◽

2019 ◽

Vol 11 (10) ◽

pp. 1293

Author(s):

Paulo Wilson Maia ◽

Marcelo Lucchesi Teixeira ◽

Luís Guilherme Scavone de Macedo ◽

Antonio Carlos Aloise ◽

Celio Amaral Passos Junior ◽

...

Keyword(s):

Bone Formation ◽

Saline Solution ◽

Healing Process ◽

Test Group ◽

The Other ◽

Control Group ◽

Collagen Membrane ◽

Platelet Rich Fibrin ◽

Histomorphometric Study ◽

Significant Difference

Platelet-rich fibrin (PRF) is an autologous material used to improve bone regeneration when associated with bone grafts. It affects tissue angiogenesis, increasing the healing process and, theoretically, presenting potential to increase bone neoformation. The aim of this study was to verify, histomorphometrically, the effects of the association of PRF to a xenograft. Twelve adult white New Zealand rabbits were randomly assigned into two groups containing six animals each. After general anesthesia of the animals, two critical defects of 12 mm were created in the rabbit calvaria, one on each side of the sagittal line. Each defect was filled with the following biomaterials: in the control group (CG), xenograft hydrated with saline solution filling one defect and xenograft hydrated with saline solution covered with collagen membrane on the other side; in the test group (TG), xenograft associated with PRF filling the defect of one side and xenograft associated with PRF covered with collagen membrane on the other side. After eight weeks the animals were euthanized and a histomorphometric analysis was performed. The results showed that in the sites that were covered with collagen membrane, there was no statistically significant difference for all the analyzed parameters. However, when comparing the groups without membrane coverage, a statistically significant difference could be observed for the vital mineralized tissue (VMT) and nonmineralized tissue (NMT) parameters, with more VMT in the test group and more NMT in the control group. Regarding the intragroup comparison, the use of the membrane coverage presented significant outcomes in both groups. Therefore, in this experimental model, PRF did not affect the levels of bone formation when a membrane coverage technique was used. However, higher levels of bone formation were observed in the test group when membrane coverage was not used.

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The Multifaceted Role of Natural Agents in Colitis-Associated Cancer Prevention and Therapy

Diagnostic and Treatment Methods for Ulcerative Colitis and Colitis-Associated Cancer - Advances in Medical Diagnosis, Treatment, and Care ◽

10.4018/978-1-7998-3580-6.ch010 ◽

2021 ◽

pp. 220-239

Author(s):

Ashok Kumar Kumar Pandurangan ◽

Suresh Kumar Anandasadagopan ◽

Neesar Ahmed

Keyword(s):

Oxidative Stress ◽

Ulcerative Colitis ◽

Inflammatory Bowel Disease ◽

Colon Cancer ◽

The Other ◽

Preclinical Model ◽

Natural Agents ◽

Inflammatory Bowel ◽

Prevention And Therapy

Inflammatory bowel disease (IBD) is comprised of ulcerative colitis (UC) and Crohn's disease (CD) that was recognized by the inflammation in the colon. There are no proper medications are available to control the IBD in patients. NASIDs such as Aspirin, diclofenac, and ibuprofen are widely used to control the inflammation. On the other hand, the untreated prolonged inflammation leads to the development of cancer in the colon termed as colitis-associated cancer or inflammation-driven colon cancer. Oxidative stress and inflammation play key roles in the pathogenesis of colitis-associated cancer. Single dose of azozymethane (AOM) and three cycles of 2% dextran sodium sulfate (DSS) induces colitis-associated cancer (CAC) in mouse. Hence, many natural products were tested in the preclinical model of colitis-associated cancer. Each of these natural agents modulate important signaling pathway to control the colitis-associated cancer (CAC). In this review, the authors tabulated all the natural agents that culminate the colitis-associated cancer in the preclinical models.

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Spontaneous and experimental poisoning of cattle by Palicourea aeneofusca in the region of Pernambuco and induction of conditioned food aversion

Ciência Rural ◽

10.1590/0103-8478cr20150079 ◽

2015 ◽

Vol 46 (1) ◽

pp. 138-143 ◽

Cited By ~ 7

Author(s):

Luiz Bezerra de Brito ◽

Raquel Feitosa Albuquerque ◽

Brena Pessoa Rocha ◽

Samuel Salgado Albuquerque ◽

Stephen Tomas Lee ◽

...

Keyword(s):

Body Weight ◽

Single Dose ◽

Lithium Chloride ◽

Test Group ◽

Aversive Conditioning ◽

Feeding Tube ◽

The Other ◽

Control Group ◽

Food Aversion ◽

Conditioned Food Aversion

ABSTRACT: The objective of this study was to describe the epidemiological, clinical, and pathological aspects of Palicourea aeneofusca poisoning in cattle in the region of Pernambuco, Brazil and to determine if it is possible to induce food aversion by P. aeneofusca poisoning in cattle raised under extensive management conditions. To determine the occurrence of poisoning, 30 properties were visited in five municipalities of the region of Pernambuco. Three outbreaks of poisoning of cattle were monitored. To induce conditioned food aversion by the consumption of P. aeneofusca, 12 animals were randomly distributed into two groups of six animals each. Cattle were weighed and received green P. aeneofusca leaves in their trough at a dose of 35mg kg-1 body weight for spontaneous consumption. The control group (CG) animals received water (1ml kg-1 body weight) via a feeding tube after the first ingestion of the plant, while the other animals, constituting the aversion test group (ATG), underwent induced aversion with lithium chloride (LiCl - 175mg kg-1 body weight) via a feeding tube. For the ATG cattle, the aversion to P. aeneofusca induced by a single dose of LiCl persisted for 12 months. In contrast, the CG animals continued to consume the plant in all tests performed, indicating the absence of aversion. This study showed that aversive conditioning using LiCl was effective in preventing poisoning by P. aeneofusca for a period of at least 12 months.

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Two Cases of Pericarditis Associated with Inflammatory Bowel Disease

Acta Medica (Hradec Kralove Czech Republic) ◽

10.14712/18059694.2018.28 ◽

2005 ◽

Vol 48 (1) ◽

pp. 43-44 ◽

Cited By ~ 2

Author(s):

Bilge Tunc ◽

Levent Filik ◽

Aysel Ulker ◽

Erkan Parlak

Keyword(s):

Ulcerative Colitis ◽

Inflammatory Bowel Disease ◽

Cardiac Disease ◽

Bowel Disease ◽

The Other ◽

Intestinal Disease ◽

Extraintestinal Manifestations ◽

Extraintestinal Manifestation ◽

Inflammatory Bowel ◽

Cardiac Manifestations

Extraintestinal manifestations are common complications of inflammatory bowel disease (IBD) whereas the association of cardiac disease with IBD is rarely reported. Cardiac manifestations may be diagnosed before, concomitantly or after the diagnosis of the specific type of inflammatory bowel disease. Pericarditis and myocarditis are potentially serious complications. This extraintestinal manifestation developed in one patient concomitantly with onset of intestinal disease. One patient had ulcerative colitis (UC), while other had Crohn’s disease (CD). Indomethacin was effective in one and the other patient required prednisone in addition. Chest symptoms in patients with inflammatory bowel disease should be evaluated to exclude myopericardial disease.

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USE OF WIDAL TEST IN DIAGNOSIS OF TYPHOID FEVER IN NORTH INDIAN POPULATION BY ESTIMATING BASELINE TITER IN CONTROL GROUP

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i7.18789 ◽

2017 ◽

Vol 10 (7) ◽

pp. 251

Author(s):

Shetty Jeevan

Keyword(s):

Typhoid Fever ◽

Healthy Volunteers ◽

Enteric Fever ◽

Early Recognition ◽

Test Group ◽

Salmonella Typhi ◽

Control Group ◽

Age Group ◽

Widal Test ◽

Medical College

Objective: This study was undertaken to establish a cutoff significant titer for Widal test using healthy volunteers as control group. Utilizing the baseline titer obtained from the control group, a diagnosis of typhoid fever was made in the test group comprising outpatients and inpatients. Methods: Blood samples were collected from healthy volunteers and patients attending G.S Medical College and Hospital, Pilkhuwa, over a period of 6 months from September 2016 to March 2017. Antibodies to Salmonella typhi (TO, TH) and Paratyphi A (AH) and Paratyphi B (BH) are determined by this tube agglutination test. A total of 124 healthy controls and 303 patients having clinical suspicion of typhoid fever were subjected to Widal test. Results: In healthy control group, titer TO ≥20 was observed in 43 (34.7%), TO ≥40 in 48 (38.7%), TO≥80 in 25 (20.2%), and titer TO ≥160, TO ≥320 was observed in none of the control group. Titer TH ≥40 in 58 (46.8%), AH ≥40 in 7 (5.6%), and BH ≥40 in 13 (10.5%) were observed in the control group. Among the test group, 96 (31.7%) sera were positive out of 303 clinically suspected enteric fever by the Widal test. Among different age group studied, 34 (46.6%) patients belonged to the age group of 11-20 years which formed the highest followed by the age group of 21-30 years (33.3%). Conclusion: Based on the study, a cutoff titer of ≥160 for anti-O and anti-H antibodies and titer of ≥80 for anti-AH and anti-BH antibodies be considered as significant titer in diagnosis of enteric fever in this region. The baseline titer helps in early recognition and treatment of this serious health problem.

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Assessment of Bacterial Profile of Ocular Infections among Subjects Undergoing Ivermectin Therapy in Onchocerciasis Endemic Area in Nigeria

Ophthalmology Research An International Journal ◽

10.9734/or/2018/v9i430094 ◽

2019 ◽

pp. 1-9

Author(s):

Okeke-Nwolisa, Benedictta Chinweoke ◽

Enweani, Ifeoma Bessie ◽

Oshim, Ifeanyi Onyema ◽

Urama, Evelyn Ukamaka ◽

Olise, Augustina Nkechi ◽

...

Keyword(s):

Visual Impairment ◽

Endemic Area ◽

Test Group ◽

Signs And Symptoms ◽

Control Group ◽

Age Group ◽

Normal Vision ◽

Bacterial Conjunctivitis ◽

Anambra State ◽

Ocular Infections

Bacteria are the major contributor of ocular infections worldwide. This can damage the structures of the eye with possible blindness and visual impairments, if left untreated. This study was undertaken to determine bacterial agents of conjunctivitis among individuals from onchocerciasis endemic area in Anambra State, Nigeria. This was a cross-sectional study involving ninety-two (92) randomly recruited test subjects undergoing ivermectin therapy aged between 11-80 years with signs and symptoms of onchocerciasis infection and bacterial conjunctivitis at Isu-Anaocha community in Anambra state between October and November, 2016. Ninety-two (92) apparently healthy subjects without signs and symptoms of onchocerciasis and bacterial conjunctivitis with normal vision, within the age of 11-80 years from the same community were used as control subjects, making a total of 184 participants. Visual acuity test was conducted on the participants using Snellen’s alphabet chart and illiterate E chart. Phenotypic and antibiotic susceptibility tests of these isolates were carried out according to Kirby - Bauer disc diffusion method guidelines. In the test group, 22 out of 92 conjunctival samples yielded bacterial growth, giving percentage prevalence of 23.9%, with age group 41-50 years having the highest frequency 7(31.8%) among individuals co-infected with onchocerciasis infection whereas 72 out of 92 conjunctival samples in the control group yielded bacterial growth giving percentage prevalence of 78.3%, with age group 41-50 years having the highest frequency 35(37.2%) among individuals that had no signs and symptoms of onchocerciasis infection. The predominant bacteria isolated was Staphylococcus aureus 8(36%) in the test group and 20 (28%) in the control group, giving a total of 28 (30%). Other bacterial isolates were Streptococcus pneumoniae 17 (18%), Staph. epidermidis 15 (16%), Escherichia coli 13(14%), Pseudomonas aeruginosa 12(13%) and Salmonella spp. 9(9%). Antibiotic susceptibility testing showed that Gentamicin and fluoroquinolones are most active against Gram positive bacteria in conjunctivitis while Augmentin was found to be the most active against Gram negative organisms, therefore, recommended as first line drug. Visual acuity (VA) test done on the test group showed that 52 (57%) persons had normal vision, 20 (22%) had mild visual impairment, 15 (16%) had moderate visual impairment, 5(5%) had severe visual impairment but none was blind. All forms of visual impairment are not wholly attributable to bacterial conjunctivitis as some could be due to ageing and uncorrected refractive errors.

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Diagnostic and Treatment Methods for Ulcerative Colitis and Colitis-Associated Cancer

Diagnostic and Treatment Methods for Ulcerative Colitis and Colitis-Associated Cancer - Advances in Medical Diagnosis, Treatment, and Care ◽

10.4018/978-1-7998-3580-6.ch004 ◽

2021 ◽

pp. 67-95

Author(s):

Syed Nasar Rahaman ◽

Prathiba Sivaprakasam ◽

Ashok Kumar Pandurangan ◽

Suresh Kumar Anandasadagopan

Keyword(s):

Ulcerative Colitis ◽

Inflammatory Bowel Disease ◽

Health Problem ◽

Bowel Disease ◽

The Other ◽

Diallyl Trisulfide ◽

Beneficial Effects ◽

Natural Agents ◽

The World ◽

Inflammatory Bowel

Ulcerative colitis (UC) is a serious health problem around the world. Inflammatory bowel disease (IBD) is comprised of both Crohn's disease (CD) and UC. IBD is a clinical condition referred as inflammation in the colon. So far there is no proper medication available to treat IBD. On the other hand, untreated UC can be developed as colitis associated cancer. Natural agents are diverse molecules possess many beneficial effects. Many researchers have proven that natural agents can be better option to treat UC. Natural agents such as chrysin, chelidonic acid, euphol, fish oil, diallyl trisulfide, embelin, isatin, and rutin were already reported to have anti-colitic activity. In this chapter, the authors documented the natural agents that were used as treatment for UC.

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Specific quality of life factors in patients with inflammatory bowel disease

Orvosi Hetilap ◽

10.1556/oh.2012.29369 ◽

2012 ◽

Vol 153 (38) ◽

pp. 1511-1519 ◽

Cited By ~ 5

Author(s):

Beáta Nagy ◽

Réka Laczkóné Majer

Keyword(s):

Quality Of Life ◽

Ulcerative Colitis ◽

Inflammatory Bowel Disease ◽

Content Analysis ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Bowel Disease ◽

Control Group ◽

Inflammatory Bowel

Introduction: Inflammatory bowel disease is a chronic disease with a fluctuating course and unknown origin. Its two major forms are Crohn’s disease and ulcerative colitis. Objective: The authors attempted to analyse the quality of life of patients with inflammatory bowel disease as compared to healthy persons and patients with asthma in order to identify specific factors which most significantly affect the quality of life of these patients. Method: 269 subjects participated in the study (control group, 115 subjects; Crohn’s disease, 67 patients; ulcerative colitis, 25 patients; asthma, 62 patients). The following methods were used: WHO-5, IIRS, SIBDQ and AQLQ(S) questionnaires and content analysis. Results: Patients with inflammatory bowel disease had the worst quality of life with respect to both subjective well-being and burden of disease. The results obtained from questionnaires developed for the analysis of quality of life indicated that extraintestinal symptoms and emotional problems are the major factors which interfere with the quality of life of patients with inflammatory bowel disease. These findings were confirmed by content analysis of interviews with patients. Conclusions: The authors recommend that more attention should be paid to improving the quality of life of patients with inflammatory bowel disease and helping them to live with the illness, especially at the level of interpersonal relations. Orv. Hetil., 2012, 153, 1511–1519.

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