scholarly journals Characterization of Components of Resistance to Corn Stunt Disease

2020 ◽  
Author(s):  
José D. Oleszczuk ◽  
María I. Catalano ◽  
Lucía Dalaisón ◽  
Julio A. Di Rienzo ◽  
María P. Giménez Pecci ◽  
...  

AbstractConsidering the occasional but increasing presence of corn stunt disease in the subtropical region of Argentina, the objective of this research was to devise an effective strategy to screen disease-resistant genotypes in the absence of high and constant natural pressures. To do so, the presence of antixenosis and antibiosis as components of resistance to vector Dalbulus maidis (DeLong 1923) as well as resistance to the pathogen Spiroplasma kunkelii (Whitcomb et al. 1986) under artificial inoculation conditions were investigated in four widely-distributed maize hybrids in Argentina. The hybrids shown differences in the levels of resistance and target organisms (either the insect vector or the pathogen). Antixenosis and antibiosis to D. maidis were observed in DK72-10. Resistance of DK79-10 to S. kunkelii was evidenced by a delayed onset of symptoms, and DKB390 was antixenotic to D. maidis and highly resistant to S. kunkelii. A good association was found between symptom severity and yield, but not between symptom severity and accumulation of pathogen S. kunkelii. In conclusion, the proposed methodology was efficacious and can aid the screening of resistant genotypes in breeding programs to reduce the impact of corn stunt disease, ensuring that hybrids with good resistance level will be planted by farmers whenever disease occurs.

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256449
Author(s):  
Muhammad Younas ◽  
Huasong Zou ◽  
Tasmia Laraib ◽  
Nasir Ahmad Rajpoot ◽  
Nasir Ahmad Khan ◽  
...  

Mungbean yellow mosaic virus (MYMV) is an important constraint in successful production of mungbean (Vigna radiata L.) in many countries, including Pakistan. The MYMV spreads by insect vector whitefly (Bemisia tabaci Gennadius). The use of resistant cultivars is the most effective management tactics for MYMV. Twenty mungbean varieties/lines were screened against insect vector of MYMV under field condition in the current study. Resistance levels for varieties/lines were assessed through visual scoring of typical disease symptoms. Furthermore, the impacts of two insecticides ‘Imidacloprid’ and ‘Thiamethoxam’ and two plant extracts, i.e., neem (Azadirachta indica), and Eucalyptus (Eucalyptus camaldulensis) were tested on the suppression of whitefly. Field screening indicated that none of the tested varieties/lines proved immune/highly resistant, while significant variations were recorded among varieties/lines for resistance level. All varieties/lines were systemically infected with MYMV. The varieties ‘AARI-2006’ and ‘Mung-14043’ were considered as resistant to MYMV based on visual symptoms and the lowest vector population. These varieties were followed by ‘NM-2006’ and ‘NL-31’, which proved as moderately resistant to MYMV. All remaining varieties/lines were grouped as moderately to highly susceptible to MYMV based on visual symptoms’ scoring. These results revealed that existing mungbean germplasm do not possess high resistance level MYMV. However, the lines showing higher resistance in the current study must be exploited in breeding programs for the development of resistant mungbean varieties/lines against MYMV. Imidacloprid proved as the most effective insecticide at all concentrations to manage whitefly population. Therefore, use of the varieties with higher resistance level and spraying Imidacloprid could lower the incidence of MYMV.


PLoS ONE ◽  
2020 ◽  
Vol 15 (10) ◽  
pp. e0234454
Author(s):  
José Darío Oleszczuk ◽  
María Inés Catalano ◽  
Lucía Dalaisón ◽  
Julio Alejandro Di Rienzo ◽  
María de la Paz Giménez Pecci ◽  
...  

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 747
Author(s):  
Tara-kay L. Jones ◽  
Raul F. Medina

Over 700 plant diseases identified as vector-borne negatively impact plant health and food security globally. The pest control of vector-borne diseases in agricultural settings is in urgent need of more effective tools. Ongoing research in genetics, molecular biology, physiology, and vector behavior has begun to unravel new insights into the transmission of phytopathogens by their insect vectors. However, the intricate mechanisms involved in phytopathogen transmission for certain pathosystems warrant further investigation. In this review, we propose the corn stunt pathosystem (Zea mays–Spiroplasma kunkelii–Dalbulus maidis) as an ideal model for dissecting the molecular determinants and mechanisms underpinning the persistent transmission of a mollicute by its specialist insect vector to an economically important monocotyledonous crop. Corn stunt is the most important disease of corn in the Americas and the Caribbean, where it causes the severe stunting of corn plants and can result in up to 100% yield loss. A comprehensive study of the corn stunt disease system will pave the way for the discovery of novel molecular targets for genetic pest control targeting either the insect vector or the phytopathogen.


1986 ◽  
Vol 36 (2) ◽  
pp. 170-178 ◽  
Author(s):  
R. F. Whitcomb ◽  
T. A. Chen ◽  
D. L. Williamson ◽  
C. Liao ◽  
J. G. Tully ◽  
...  

2020 ◽  
Vol 4 (2) ◽  
pp. 118-129
Author(s):  
Asti Gumartifa ◽  
◽  
Indah Windra Dwie Agustiani

Gaining English language learning effectively has been discussed all years long. Similarly, Learners have various troubles outcomes in the learning process. Creating a joyful and comfortable situation must be considered by learners. Thus, the implementation of effective learning strategies is certainly necessary for English learners. This descriptive study has two purposes: first, to introduce the classification and characterization of learning strategies such as; memory, cognitive, metacognitive, compensation, social, and affective strategies that are used by learners in the classroom and second, it provides some questionnaires item based on Strategy of Inventory for Language Learning (SILL) version 5.0 that can be used to examine the frequency of students’ learning strategies in the learning process. The summary of this study explains and discusses the researchers’ point of view on the impact of learning outcomes by learning strategies used. Finally, utilizing appropriate learning strategies are certainly beneficial for both teachers and learners to achieve the learning target effectively.


2020 ◽  
Vol 7 ◽  
Author(s):  
Xupeng Yuan ◽  
Jiahao Yan ◽  
Ruizhi Hu ◽  
Yanli Li ◽  
Ying Wang ◽  
...  

Recent evidences suggest that gut microbiota plays an important role in regulating physiological and metabolic activities of pregnant sows, and β-carotene has a potentially positive effect on reproduction, but the impact of β-carotene on gut microbiota in pregnant sows remains unknown. This study aimed to explore the effect and mechanisms of β-carotene on the reproductive performance of sows from the aspect of gut microbiota. A total of 48 hybrid pregnant sows (Landrace × Yorkshire) with similar parity were randomly allocated into three groups (n = 16) and fed with a basal diet or a diet containing 30 or 90 mg/kg of β-carotene from day 90 of gestation until parturition. Dietary supplementation of 30 or 90 mg/kg β-carotene increased the number of live birth to 11.82 ± 1.54 and 12.29 ± 2.09, respectively, while the control group was 11.00 ± 1.41 (P = 0.201). Moreover, β-carotene increased significantly the serum nitric oxide (NO) level and glutathione peroxidase (GSH-Px) activity (P < 0.05). Characterization of fecal microbiota revealed that 90 mg/kg β-carotene increased the diversity of the gut flora (P < 0.05). In particular, β-carotene decreased the relative abundance of Firmicutes including Lachnospiraceae AC2044 group, Lachnospiraceae NK4B4 group and Ruminococcaceae UCG-008, but enriched Proteobacteria including Bilophila and Sutterella, and Actinobacteria including Corynebacterium and Corynebacterium 1 which are related to NO synthesis. These data demonstrated that dietary supplementation of β-carotene may increase antioxidant enzyme activity and NO, an important vasodilator to promote the neonatal blood circulation, through regulating gut microbiota in sows.


Blood ◽  
2019 ◽  
Vol 133 (13) ◽  
pp. 1436-1445 ◽  
Author(s):  
Jyoti Nangalia ◽  
Emily Mitchell ◽  
Anthony R. Green

Abstract Interrogation of hematopoietic tissue at the clonal level has a rich history spanning over 50 years, and has provided critical insights into both normal and malignant hematopoiesis. Characterization of chromosomes identified some of the first genetic links to cancer with the discovery of chromosomal translocations in association with many hematological neoplasms. The unique accessibility of hematopoietic tissue and the ability to clonally expand hematopoietic progenitors in vitro has provided fundamental insights into the cellular hierarchy of normal hematopoiesis, as well as the functional impact of driver mutations in disease. Transplantation assays in murine models have enabled cellular assessment of the functional consequences of somatic mutations in vivo. Most recently, next-generation sequencing–based assays have shown great promise in allowing multi-“omic” characterization of single cells. Here, we review how clonal approaches have advanced our understanding of disease development, focusing on the acquisition of somatic mutations, clonal selection, driver mutation cooperation, and tumor evolution.


Author(s):  
Barbara Ludwig Navarro ◽  
Lucia Ramos Romero ◽  
María Belén Kistner ◽  
Juliana Iglesias ◽  
Andreas von Tiedemann

AbstractNorthern corn leaf blight (NCLB) is one of the most important diseases in maize worldwide. It is caused by the fungus Exserohilum turcicum, which exhibits a high genetic variability for virulence, and hence physiological races have been reported. Disease control is based mainly on fungicide application and host resistance. Qualitative resistance has been widely used to control NCLB through the deployment of Ht genes. Known pathogen races are designated according to their virulence to the corresponding Ht gene. Knowledge about of E. turcicum race distribution in maize-producing areas is essential to develop and exploit resistant genotypes. Maize leaves showing distinct elliptical grey-green lesions were collected from maize-producing areas of Argentina and Brazil, and 184 monosporic E. turcicum isolates were obtained. A total of 66 isolates were collected from Argentina during 2015, 2018 and 2019, while 118 isolates from Brazil were collected during 2017, 2018 and 2019. All isolates were screened on maize differential lines containing Ht1, Ht2, Ht3 and Htn1 resistance genes. In greenhouse experiments, inoculated maize plants were evaluated at 14 days after inoculation. Resistance reaction was characterized by chlorosis, and susceptibility was defined by necrosis in the absence of chlorosis. The most frequent race was 0 in both Argentina (83%) and Brazil (65%). Frequencies of race 1 (6% and 24%) and race 23N (5% and 10%) were very low in Argentina and Brazil, respectively. The high frequency of race 0 isolates provides evidence that qualitative resistance based on the tested Ht genes is not being used extensively in Argentina and Brazil to control NCLB. This information may be relevant for growers and breeding programs as the incidence of NCLB is increasing in both countries.


2021 ◽  
Vol 13 (8) ◽  
pp. 4105
Author(s):  
Yupei Jiang ◽  
Honghu Sun

Leisure walking has been an important topic in space-time behavior and public health research. However, prior studies pay little attention to the integration and the characterization of diverse and multilevel demands of leisure walking. This study constructs a theoretical framework of leisure walking behavior demands from three different dimensions and levels of activity participation, space-time opportunity, and health benefit. On this basis, through a face-to-face survey in Nanjing, China (N = 1168, 2017–2018 data), this study quantitatively analyzes the characteristics of leisure walking demands, as well as the impact of the built environment and individual factors on it. The results show that residents have a high demand for participation and health benefits of leisure walking. The residential neighborhood provides more space opportunities for leisure walking, but there is a certain constraint on the choice of walking time. Residential neighborhood with medium or large parks is more likely to satisfy residents’ demands for engaging in leisure walking and obtaining high health benefits, while neighborhood with a high density of walking paths tends to limit the satisfaction of demands for space opportunity and health benefit. For residents aged 36 and above, married, or retired, their diverse demands for leisure walking are more likely to be fulfilled, while those with high education, medium-high individual income, general and above health status, or children (<18 years) are less likely to be fulfilled. These finding that can have important implications for the healthy neighborhood by fully considering diverse and multilevel demands of leisure walking behavior.


Author(s):  
Weiqi Xu ◽  
Chun Chen ◽  
Yanmei Qiu ◽  
Conghui Xie ◽  
Yunle Chen ◽  
...  

Organic aerosol (OA), a large fraction of fine particles, has a large impact on climate radiative forcing and human health, and the impact depends strongly on size distributions. Here we...


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