scholarly journals Sulfur limitation increases duckweed starch accumulation without compromising growth

2021 ◽  
Author(s):  
Zuoliang Sun ◽  
Wenjun Guo ◽  
Xuyao Zhao ◽  
Jingjing Yang ◽  
Pengfei Duan ◽  
...  
Keyword(s):  
Nitrogen Limitation ◽  
Performance Indicator ◽  
Starch Content ◽  
Phosphorus Limitation ◽  
Biofuel Production ◽  
High Yield ◽  
Starch Accumulation ◽  
List Type ◽  
Starch Yield ◽  
Starch Production

AbstractDuckweeds contain relatively high levels of starch and are a potential biomass feedstock for biofuel production. Here, the biomass and starch yield of duckweed under three different nutrient-limited conditions were analyzed to investigate possible ways of further increasing the efficiency of starch production. The results showed that sulfur limitation resulted in the highest starch yield, which was 42% and 73% higher than in nitrogen or phosphorus limitation, respectively. The high yield of sulfur-limited duckweed is largely due to the combinations of little effects on biomass and high accumulations of starch. Although nitrogen limitation led to higher starch content (67.4%), it severely reduced biomass production. The photosynthetic performance indicator Fv/Fm was a simple and sensitive indicator of starch content in nutrient-limited duckweed. Taken together, this study demonstrates that sulfur limitation is a simple and efficient way to increase starch yield, highlighting the great potential of duckweed for biofuel production. We report that sulfur limitation is a practical approach to increase starch yields in duckweed without affecting growth or biomass.HighlightsSulfur limitation induces starch production in a duckweed specie.Nitrogen limitation triggers the highest starch content, but limits growth.Sulfur limitation results in the highest starch yield.Fv/Fm is a rapid and robust proxy of starch content in nutrient-limited duckweed.

scholarly journals Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1084
Author(s):  
Ivan N. Ivanov ◽  
Vilém Zachleder ◽  
Milada Vítová ◽  
Maria J. Barbosa ◽  
Kateřina Bišová
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Starch Content ◽  
Light Availability ◽  
Fold Increase ◽  
Pilot Scale ◽  
Temperature Treatment ◽  
Control Culture ◽  
Starch Accumulation ◽  
Cellular Division ◽  
Starch Production

An increase in temperature can have a profound effect on the cell cycle and cell division in green algae, whereas growth and the synthesis of energy storage compounds are less influenced. In Chlamydomonas reinhardtii, laboratory experiments have shown that exposure to a supraoptimal temperature (39 °C) causes a complete block of nuclear and cellular division accompanied by an increased accumulation of starch. In this work we explore the potential of supraoptimal temperature as a method to promote starch production in C. reinhardtii in a pilot-scale photobioreactor. The method was successfully applied and resulted in an almost 3-fold increase in the starch content of C. reinhardtii dry matter. Moreover, a maximum starch content at the supraoptimal temperature was reached within 1–2 days, compared with 5 days for the control culture at the optimal temperature (30 °C). Therefore, supraoptimal temperature treatment promotes rapid starch accumulation and suggests a viable alternative to other starch-inducing methods, such as nutrient depletion. Nevertheless, technical challenges, such as bioreactor design and light availability within the culture, still need to be dealt with.

scholarly journals Molecular mechanism underlying the effect of maleic hydrazide treatment on starch accumulation in S. polyrrhiza 7498 fronds

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yerong Zhu ◽  
Xiaoxue Li ◽  
Xuan Gao ◽  
Jiqi Sun ◽  
Xiaoyuan Ji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Carbon Fixation ◽  
Maleic Hydrazide ◽  
Starch Content ◽  
Vegetative Reproduction ◽  
Photosynthetic Pigment ◽  
Starch Accumulation ◽  
Chlorophyll Fluorescence Parameters ◽  
Significant Difference ◽  
Starch Production

Abstract Background Duckweed is considered a promising feedstock for bioethanol production due to its high biomass and starch production. The starch content can be promoted by plant growth regulators after the vegetative reproduction being inhibited. Maleic hydrazide (MH) has been reported to inhibit plant growth, meantime to increase biomass and starch content in some plants. However, the molecular explanation on the mechanism of MH action is still unclear. Results To know the effect and action mode of MH on the growth and starch accumulation in Spirodela polyrrhiza 7498, the plants were treated with different concentrations of MH. Our results showed a substantial inhibition of the growth in both fronds and roots, and increase in starch contents of plants after MH treatment. And with 75 µg/mL MH treatment and on the 8th day of the experiment, starch content was the highest, about 40 mg/g fresh weight, which is about 20-fold higher than the control. The I2-KI staining and TEM results confirmed that 75 µg/mL MH-treated fronds possessed more starch and big starch granules than that of the control. No significant difference for both in the photosynthetic pigment content and the chlorophyll fluorescence parameters of PII was found. Differentially expressed transcripts were analyzed in S. polyrrhiza 7498 after 75 µg/mL MH treatment. The results showed that the expression of some genes related to auxin response reaction was down-regulated; while, expression of some genes involved in carbon fixation, C4 pathway of photosynthesis, starch biosynthesis and ABA signal transduction pathway was up-regulated. Conclusion The results provide novel insights into the underlying mechanisms of growth inhibition and starch accumulation by MH treatment, and provide a selective way for the improvement of starch production in duckweed.

scholarly journals Seasonal Variation in Starch Accumulation and Starch Granule Size in Cassava Genotypes in a Tropical Savanna Climate

Agronomy ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 297 ◽  
Author(s):  
Anon Janket ◽  
Nimitr Vorasoot ◽  
Banyong Toomsan ◽  
Wanwipa Kaewpradit ◽  
Poramate Banterng ◽  
...  
Keyword(s):  
Seasonal Variation ◽  
Starch Granule ◽  
Amylose Content ◽  
Starch Content ◽  
Block Design ◽  
Granule Size ◽  
Starch Accumulation ◽  
Planting Dates ◽  
High Starch ◽  
Starch Yield

The information on genotypic responses to the seasonal variation in the starch content, starch yield, and starch quality of cassava is limited. The objective of this research was to investigate the seasonal variation of starch characteristics of three cassava genotypes grown under irrigation. The experiment was conducted at four planting dates (20 April, 30 June, 5 October, and 15 December 2015). Three cassava genotypes (CMR38-125-77, Kasetsart 50, and Rayong 11) were evaluated in a randomized complete block design with four replications and the plants were harvested at 12 months. The planting date contributed the largest portion of the total variation in the starch content, starch yield, and starch granule size. The amylose content variability was heavily influenced by genotype. Cassava planted on 5 October or 15 December had greater starch content, starch yield, and starch granule in most genotypes. This was likely due to a higher temperature and solar radiation during the 3–9 months post-planting. CMR38-125-77 showed a consistently high starch content, starch yield, and high amylose content for most planting dates except for the starch yield on 20 April, of which Rayong 11 was the best. These findings will be useful for choosing suitable cassava genotypes for different growing seasons and for facilitating breeding efforts for high starch-yielding and high-quality cassava starch in the future.

scholarly journals Starch Accumulation and Granule Size Distribution of Cassava cv. Rayong 9 Grown under Irrigated and Rainfed Conditions Using Different Growing Seasons

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 412 ◽  
Author(s):  
Anon Janket ◽  
Nimitr Vorasoot ◽  
Banyong Toomsan ◽  
Wanwipa Kaewpradit ◽  
Sanun Jogloy ◽  
...  
Keyword(s):  
Starch Granule ◽  
Amylose Content ◽  
Starch Content ◽  
Granule Size ◽  
Dry Season ◽  
Starch Accumulation ◽  
Planting Dates ◽  
Hot Dry Season ◽  
Rainfed Conditions ◽  
Starch Yield

Although cassava can be planted throughout the year, its starch qualities may vary based on the date of planting. Seasonal variation on starch content, starch yield, starch granule size and amylose content of cassava cv. Rayong 9 grown under irrigated and rainfed conditions were studied for four planting dates in Thailand. A randomized complete block design with four replications was used in each planting. Planting dates consisted of hot-dry (20-Apr), early-rainy (30-Jun), late-rainy (5-Oct) and cool seasons (15-Dec). At final harvest, planting date accounted for the largest variations for starch yield (60.8%), starch granule size (38.2%), amylose content (50.5%) and ratio of amylose to amylopectin (53.7%), whereas starch content was affected more by water regime (52.1%). Supplemental irrigation did not significantly increase starch yield and other parameters for most planting dates, except for starch yield of the crop planted in the hot-dry season. This indicated that irrigation at the late-growth stages (during Sep to Mar) for the crop planted in the hot-dry season helped to increase starch yield; however, irrigation was unnecessary for other planting dates once cassava was established. The crops planted in the late-rainy and cool seasons had a greater starch content and starch yield than other planting dates for both irrigated and rainfed crops, whereas the crop planted in the hot-dry season had high starch yield for the irrigated crops only. In this study, the crops planted in the early-rainy season showed the worst performances for starch content and starch yield for both irrigated and rainfed crops. The data provided information on the responses of starch yield and its characteristics under irrigated and rainfed conditions at different planting dates, which can be useful for designing cultural practices with respect to water management and planting period in order to obtain optimum starch yield and qualities.

Co-expression of AGPS and AGPL genes encoded for AGPase from cassava to enhance starch accumulation in transgenic tobacco

2016 ◽  
Vol 14 (2) ◽  
pp. 287-293
Author(s):  
Nguyễn Văn Đoài ◽  
Nguyễn Minh Hồng ◽  
Lê Thu Ngọc ◽  
Nguyễn Thị Thơm ◽  
Nguyễn Đình Trọng ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Higher Plants ◽  
Starch Content ◽  
Genetically Modified Crops ◽  
Starch Accumulation ◽  
35S Promoter ◽  
Effective Strategies ◽  
Plant Expression Vector ◽  
Transgenic Lines ◽  
Cassava Varieties

The AGPase (ADP-Glucose pyrophosphorylase) is one of the ubiquitous enzymes catalyzing the first step in starch biosynthesis. It plays an important role in regulation and adjusts the speed of the entire cycle of glycogen biosynthesis in bacteria and starch in plants. In higher plants, it is a heterotetramer and tetrameric enzyme consisting two large subunits (AGPL) and two small subunits (AGPS) and encoded by two genes. In this paper, both AGPS and AGPL genes were sucessfully isolated from cassava varieties KM140 and deposited in Genbank with accession numbers KU243124 (AGPS) and KU243122 (AGPL), these two genes were fused with P2a and inserted into plant expression vector pBI121 under the control of 35S promoter. The efficient of this construct was tested in transgenic N. tabacum. The presence and expression of AGPS and AGPL in transgenic plants were confirmed by PCR and Western hybridization. The starch content was quantified by the Anthrone method. Transgenic plant analysis indicated that that two targeted genes were expressed simultaneously in several transgenic tobacco lines under the control of CaMV 35S promoter.  The starch contents in 4 analyzed tobacco transgenic lines displays the increase 13-116%  compared to WT plants. These results indicated that the co-expression of AGPS and AGPL is one of effective strategies for enhanced starch production in plant. These results can provide a foundation for developing other genetically modified crops to increase starch accumulation capacity.

Optimization of the Fermentation Conditions for 1-Deoxynojirimycin Production by Streptomyces lawendulae Applying the Response Surface Methodology

2011 ◽  
Vol 7 (3) ◽  
Author(s):  
Zhao-Jun Wei ◽  
Le-Chun Zhou ◽  
Hua Chen ◽  
Gui-Hai Chen
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Starch Content ◽  
Ultra Violet ◽  
Soluble Starch ◽  
High Yield ◽  
Yield Strain ◽  
Fermentation Time ◽  
Ethyl Sulfate ◽  
Initial Ph

Moranoline (1-Deoxynojirimycin, DNJ) is a piperidine alkaloid, and shows high inhibit activities to glucoamylase and ?-glucosidase. One DNJ high-yield strain of Streptomyces lawendulae was obtained after isolated form soil and mutated with the ultra violet (UV) and ethyl sulfate (DES), which named as TB-412, and can produce DNJ with 35.925 mg/L. Response surface methodology (RSM) was applied to optimize the parameters of DNJ yield from S. lawendulae TB-412. The effects of independent variables of fermentation, including time, temperature, initial pH and the soluble starch content were investigated. The statistical analysis showed that the fermentation time, pH and the soluble starch content, and the quadratics of time, temperature, pH and the soluble starch content, as well as the interactions between fermentation time and pH, and time and the soluble starch content, showed significant effects on DNJ yield. The optimal process parameters for DNJ production within the experimental range of the variables researched was at 11d, 27 °C, pH 7.5, and 8% soluble starch content. At this condition, the DNJ yield was predicted to be 42.875 mg/L.

scholarly journals POTENCIAL DE CLONES EXPERIMENTAIS DE BATATA-DOCE PARA PRODUÇÃO DE ETANOL

Nativa ◽  
2018 ◽  
Vol 6 (4) ◽  
pp. 352
Author(s):  
Adriano Mendes Lourenço ◽  
Aline Torquato Tavares ◽  
Tiago Alves Ferreira ◽  
Danilo Alves da Silva Porto Lopes ◽  
João Victor Gonçalves Carline ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Ethanol Yield ◽  
Starch Content ◽  
Great Ability ◽  
Ethanol Yields ◽  
Root Starch ◽  
Breeding Selection ◽  
Starch Yield

A batata-doce (Ipomoea batatas (L.) Lam.) tem sido reportada como uma das espécies de planta com grande capacidade de converter biomassa em matéria prima para produção de etanol. O objetivo do trabalho foi avaliar o potencial de clones de batata-doce para produção de etanol. Foram avaliados 60 clones de batata-doce para produtividade de raízes, teor de amido nas raízes, produtividade de amido, coloração da casca e da polpa e o rendimento de etanol. O clone BDTO#122,32 e as cultivares Ana Clara e Carolina Vitória com média de 46,77; 42,75 e 41,25 t ha-¹, respectivamente, foram os que mais conseguiram acumular biomassa na forma de raiz. Os clones que apresentam as maiores médias de produtividade de amido por hectare foram BDTO#144.22 e BDTO#100.23, com valores de 15,46 e 14,16% t ha-1, com rendimentos de etanol de 8,33 e 7,63 m³ ha-¹. Os clones BDTO#144.22 e BDTO#100.23 apresentaram as maiores médias de produtividade de amido por hectare e rendimento de etanol, sendo, portanto, os mais promissores para a produção de etanol.Palavras-chave: Ipomoea batatas (L.) Lam, melhoramento genético, seleção, biocombustível. POTENTIAL OF EXPERIMENTAL CLONES OF SWEET POTATO FOR ETHANOL PRODUCTION ABSTRACT:Sweet potato (Ipomoea batatas (L.) Lam.) Has been reported as one of the plant species with great ability to convert biomass into feedstock for ethanol production. The objective of this work was to evaluate the potential of sweet potato clones for ethanol production. Twenty-six sweet potato clones were evaluated for root productivity, root starch content, starch yield, bark and pulp color, and ethanol yield. Clone BDTO # 122.32 and cultivars Ana Clara and Carolina Vitória averaging 46.77; 42.75 and 41.25 t ha-1, respectively, were the ones that were able to accumulate biomass in the root form. The clones presenting the highest starch productivity per hectare were BDTO # 144.22 and BDTO # 100.23, with values of 15.46 and 14.16% t ha-1, with ethanol yields of 8.33 and 7.63 m³ ha-¹. The clones BDTO # 144.22 and BDTO # 100.23 showed the highest averages of starch productivity per hectare and yield of ethanol, thus being the most promising for the production of ethanol.Keywords: Ipomoea potatoes (L.) Lam, breeding, selection, biofuel.

scholarly journals Effect of Thermal Pretreatments on Phosphorylation of Corypha umbraculifera L. Stem Pith Starch: A Comparative Study Using Dry-Heat, Heat-Moisture and Autoclave Treatments

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3855
Author(s):  
Basheer Aaliya ◽  
Kappat Valiyapeediyekkal Sunooj ◽  
Chillapalli Babu Sri Rajkumar ◽  
Muhammed Navaf ◽  
Plachikkattu Parambil Akhila ◽  
...  
Keyword(s):  
Amylose Content ◽  
Starch Content ◽  
Light Transmittance ◽  
High Yield ◽  
Thermal Treatments ◽  
Relative Crystallinity ◽  
Dry Heat ◽  
Modified Starches ◽  
Starch Gel ◽  
Phosphorous Content

Talipot starch, a non-conventional starch source with a high yield (76%) from the stem pith of talipot palm (Corypha umbraculifera L.) was subjected to three different thermal treatments (dry-heat, heat-moisture and autoclave treatments) prior to phosphorylation. Upon dual modification of starch with thermal treatments and phosphorylation, the phosphorous content and degree of crosslinking significantly increased (p ≤ 0.05) and was confirmed by the increased peak intensity of P=O and P–O–C stretching vibrations compared to phosphorylated talipot starch in the FT-IR spectrum. The highest degree of crosslinking (0.00418) was observed in the autoclave pretreated phosphorylated talipot starch sample. Thermal pretreatment remarkably changed the granule morphology by creating fissures and grooves. The amylose content and relative crystallinity of all phosphorylated talipot starches significantly decreased (p ≤ 0.05) due to crosslinking by the formation of phosphodiester bonds, reducing the swelling power of dual-modified starches. Among all modified starches, dry-heat pretreated phosphorylated starch gel showed an improved light transmittance value of 28.4%, indicating reduced retrogradation tendency. Pasting and rheological properties represented that the thermal pretreated phosphorylated starch formed stronger gels that improved thermal and shear resistance. Autoclave treatment before phosphorylation of talipot starch showed the highest resistant starch content of 48.08%.

scholarly journals Antioxidant gene expression, chlorophyll, and starch content in Persian lime (Citrus latifolia Tanaka Ex Q. Jiménez) trees with HLB by application of elicitors of plant resistance

2021 ◽  
Vol 6 (2) ◽  
pp. 86-102
Author(s):  
Felipe Roberto Flores-de la Rosa ◽  
◽  
Ricardo Santillán-Mendoza ◽  
Cynthia Guadalupe Rodríguez-Quibrera ◽  
Antonio Martínez-Ruiz ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Starch Content ◽  
Starch Accumulation ◽  
Economic Losses ◽  
Total Chlorophyll ◽  
Gamma Aminobutyric Acid ◽  
Antioxidant Genes ◽  
Spectrophotometric Methods ◽  
Total Chlorophyll Content ◽  
Randomized Design

Persian lime is economically important for Mexico. However, the disease known as Huanglongbing (HLB) causes chlorosis symptoms in the foliage that affect the growth and decreases the yield of the trees. The expression of chlorosis symptoms is associated with starch accumulation and inhibition of antioxidant activity. The objective of the present work was to determine the effect of the application of three resistance elicitors on the expression of three antioxidant genes and on starch and chlorophyll content in Persian lime trees with HLB. Salicylic acid (SA), jasmonic acid (JA) or gamma-aminobutyric acid (GABA) were applied every eight days for nine weeks using a completely randomized design and a one-way ANOVA was performed for the analysis. Expression of APX, CAT and SOD genes was quantified at week nine at four different times. Starch and total chlorophyll content was estimated every three weeks by spectrophotometric methods. The application of the elicitors significantly increased the expression of the three genes, with SA and GABA generating the greatest increase at different times of application. No difference was found in starch and total chlorophyll content at most of the times evaluated. The use of resistance elicitors is promising in the management of HLB in plots already infected, seeking to extend the productive life of the orchards and thus counteract the economic losses caused by HLB.

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