scholarly journals Improved Sensitivity in Low-Input Proteomics using Micro-Pillar Array-based Chromatography

2019 ◽  
Author(s):  
Johannes Stadlmann ◽  
Otto Hudecz ◽  
Gabriela Krššáková ◽  
Gert Van Raemdonck ◽  
Jeff Op De Beeck ◽  
...  
Keyword(s):  
Spherical Particles ◽  
Pillar Array ◽  
Low Input ◽  
Support Materials ◽  
Current State ◽  
Complex Protein ◽  
Protein Group ◽  
Technological Improvements ◽  
Made In

ABSTRACTCapitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently one of the most sensitive analytical technologies for the comprehensive characterization of complex protein samples.However, despite tremendous technological improvements made in the production and the packing of monodisperse spherical particles for nano-flow HPLC, current state-of-the-art systems still suffer from limits in operation at the maximum potential of the technology.With the recent introduction of the µPAC system, which provides perfectly ordered micro-pillar array based chromatographic support materials, completely new chromatographic concepts for optimization towards the needs of ultra-sensitive proteomics become available.Here we report on a series of benchmarking experiments comparing the performance of a commercially available 50 cm micro-pillar array column to a widely used nano-flow HPLC column for the proteomics analysis of 10 ng tryptic HeLa cell digest.Comparative analysis of LC-MS/MS-data corroborated that micro-pillar array cartridges provide outstanding chromatographic performance, excellent retention time stability, increase sensitivity in the analysis of low-input proteomics samples, and thus repeatedly yielded almost twice as many unique peptide and unique protein group identifications when compared to conventional nano-flow HPLC columns.

scholarly journals Metabolite transport across the peroxisomal membrane

2006 ◽  
Vol 401 (2) ◽  
pp. 365-375 ◽  
Author(s):  
Wouter F. Visser ◽  
Carlo W. T. van Roermund ◽  
Lodewijk Ijlst ◽  
Hans R. Waterham ◽  
Ronald J. A. Wanders
Keyword(s):  
Fatty Acid ◽  
Metabolic Pathways ◽  
Adenine Nucleotides ◽  
Transport Systems ◽  
Peroxisomal Membrane ◽  
Metabolite Transport ◽  
Metabolic Functions ◽  
Current State ◽  
Made In

In recent years, much progress has been made with respect to the unravelling of the functions of peroxisomes in metabolism, and it is now well established that peroxisomes are indispensable organelles, especially in higher eukaryotes. Peroxisomes catalyse a number of essential metabolic functions including fatty acid β-oxidation, ether phospholipid biosynthesis, fatty acid α-oxidation and glyoxylate detoxification. The involvement of peroxisomes in these metabolic pathways necessitates the transport of metabolites in and out of peroxisomes. Recently, considerable progress has been made in the characterization of metabolite transport across the peroxisomal membrane. Peroxisomes posses several specialized transport systems to transport metabolites. This is exemplified by the identification of a specific transporter for adenine nucleotides and several half-ABC (ATP-binding cassette) transporters which may be present as hetero- and homo-dimers. The nature of the substrates handled by the different ABC transporters is less clear. In this review we will describe the current state of knowledge of the permeability properties of the peroxisomal membrane.

scholarly journals Revision of some species of the genus Cryptosporidium (Tyzzer, 1907) (Eucoccidiorida, cryptosporidiidae) in cattle in Iraq

2021 ◽  
Vol 14 (1) ◽  
pp. 116-120
Author(s):  
Zainab A. Makawi ◽  
Hind D Hadi ◽  
Azhar Ali
Keyword(s):  
Virulence Factors ◽  
Protozoan Parasite ◽  
Molecular Techniques ◽  
Host Susceptibility ◽  
Susceptibility To Infection ◽  
Current State ◽  
Vertebrate Hosts ◽  
Important Progress ◽  
Made In

Cryptosporidium is a protozoan parasite of medical and veterinary significance that causes gastroenteritis in a number of vertebrate hosts. Several studies have recorded different degrees of pathogenicity and virulence among Cryptosporidium species and isolates of the same species as well as evidence of variation in host susceptibility to infection. Nevertheless, important progress has been made in determining Cryptosporidium's putative virulence factors. Since the publication of C parvum and C. Hominis this development has been accelerated genomes, identified by a range of immunological and molecular techniques with the characterization of over 25 putative virulence factors, which are proposed to be involved in aspects of host-pathogen interactions from adhesion and locomotion to invasion and proliferation. There has also been improvement in the contribution of host variables correlated with differences in both the severity and risk of infection. In view of our current understanding of microbial virulence, we present a summary of the current state of information on Cryptosporidium infectivity, pathogenesis, and transmissibility here.

Muhammad Nejatullah Siddiqui. Issues in Islamic Banking: Selected Papers, Leicester: The Islamic Foundation. 1983 , I 52pp.Author and Subject Indexes.

1983 ◽  
Vol 22 (1) ◽  
pp. 57-61
Author(s):  
Shahrukh Rafi Khan
Keyword(s):  
Analytical Model ◽  
Significant Contribution ◽  
Profit Sharing ◽  
Islamic Banking ◽  
Current State ◽  
The Subject ◽  
Made In

The book under review is a compilation of the author's articles and lectures that highlight the prominent developments in the literature on the subject of Islamic banking and inform the reader of the current state of debate on it. One of the earliest and main contributors to this topic is the author himself. The focus of this review will mainly be on "Economics of Profit-Sharing", which is the title of the fourth chapter of the book and is among his latest contributions. This chapter is a significant contribution as it is the first attempt to formalise the concept of profit sharing into an analytical model and, therefore, demands closer scrutiny. However, in the remaining chapters of the book, the author has drawn attention to some of the fine points made in the literature on this topic. Since some of these points appear to be controversial to me, I will briefly discuss them before moving on to the analytical chapter of the book.

Bioactivity of Marine Natural Product Xyloketals

2020 ◽  
Vol 17 ◽  
Author(s):  
Biswajit Panda ◽  
Amal Kumar Gooyee
Keyword(s):  
Natural Products ◽  
Marine Natural Product ◽  
Ischemic Brain Injury ◽  
Ischemic Brain ◽  
Activity Inhibition ◽  
Life Saving ◽  
Behavior Suppression ◽  
Major Emphasis ◽  
Made In

: Oceans can play a major role in supplying life-saving medicines in the world in future. Although considerable progress has been made in finding new medicines from marine sources, large efforts are still necessary to examine such molecules for clinical applications. Xyloketals are an important group of natural products with various powerful and prominent bioactivities such as inhibition of acetylcholine esterase, antioxidant activity, inhibition of L-calcium channels, radicalscavenging behavior, suppression of cell proliferation, reduction of neonatal hypoxic-ischemic brain injury, etc. This review describes the isolation and structural characterization of all xyloketal natural products giving major emphasis on their bioactivity.

Characterization of Auditory Disability and Its Relation to the Resilience

2018 ◽  
Vol 5 (2) ◽  
pp. 15
Author(s):  
Bibian Bibeca Bumbila García ◽  
Hernán Andrés Cedeño Cedeño ◽  
Tatiana Moreira Chica ◽  
Yaritza Rossana Parrales Ríos
Keyword(s):  
Social Integration ◽  
Conceptual Analysis ◽  
Disabled People ◽  
Disabled Students ◽  
Hearing Disability ◽  
Technical University ◽  
The Relationship ◽  
Made In

The objective of the work is to establish the characterization of the auditory disability and its relationship with resilience at the Technical University of Manabí. The article shows a conceptual analysis related to the inclusion and social integration of disabled students. Based on the fact that the person with disabilities grows and develops in the same way as that of people without disabilities and what usually happens is that disabled people are rejected and discriminated against based on a prefabricated and erroneous conceptualization of these people. The results associated with the application of the SV-RES test prepared by the researchers are shown (Saavedra & Villalta, 2008b). Characterization of the auditory deficit is made in the students, and the limitations that derive from it are pointed out. We analyze the particularities related to communication with students who have a hearing disability and resilience in this type of student, where some personal highlights that in this sense constitute an example of resilience. Finally, the results related to the study of the relationship between students' hearing disability and the level of resilience dimensions are shown.

scholarly journals Deep Learning Based Instance Segmentation of Titanium Dioxide Particles in the Form of Agglomerates in Scanning Electron Microscopy

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 968
Author(s):  
Paul Monchot ◽  
Loïc Coquelin ◽  
Khaled Guerroudj ◽  
Nicolas Feltin ◽  
Alexandra Delvallée ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Titanium Dioxide ◽  
Learning Community ◽  
Spherical Particles ◽  
Average Value ◽  
Size Characterization ◽  
Scanning Electron

The size characterization of particles present in the form of agglomerates in images measured by scanning electron microscopy (SEM) requires a powerful image segmentation tool in order to properly define the boundaries of each particle. In this work, we propose to use an algorithm from the deep statistical learning community, the Mask-RCNN, coupled with transfer learning to overcome the problem of generalization of the commonly used image processing methods such as watershed or active contour. Indeed, the adjustment of the parameters of these algorithms is almost systematically necessary and slows down the automation of the processing chain. The Mask-RCNN is adapted here to the case study and we present results obtained on titanium dioxide samples (non-spherical particles) with a level of performance evaluated by different metrics such as the DICE coefficient, which reaches an average value of 0.95 on the test images.

scholarly journals Drug delivery applications of poly-γ-glutamic acid

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Olalekan A. Balogun-Agbaje ◽  
Olubusola A. Odeniyi ◽  
Michael A. Odeniyi
Keyword(s):  
Drug Delivery ◽  
Glutamic Acid ◽  
Site Characterization ◽  
Fermentation Medium ◽  
Production Optimization ◽  
Main Body ◽  
Crucial Step ◽  
Drug Delivery Application ◽  
Made In

Abstract Background Poly-γ-glutamic acid (γ-PGA) is a biopolymer of microbial origin, consisting of repeating units of l-glutamic acid and/or D-glutamic acid. The biopolymer has found use in the fields of agriculture, food, wastewater, and medicine, owing to its non-toxic, biodegradable, and biocompatible properties. Due to its biodegradability, γ-PGA is being tipped to dislodge synthetic plastics in drug delivery application. High cost of production, relative to plastics, is however a clog in the wheel of achieving this. Main body of abstract This review looked at the production, nanoparticles fabrication, and drug delivery application of γ-PGA. γ-PGA production optimization by modifying the fermentation medium to tailor towards the production of desirable polymer at reduced cost and techniques for the formulation of γ-PGA nanoparticle as well as its characterization were discussed. This review also evaluated the application of γ-PGA and its nanoparticles in the delivery of drugs to action site. Characterization of γ-PGA and its nanoparticles is a crucial step towards determining the applicability of the biopolymer. γ-PGA has been used in the delivery of active agents to action sites. Conclusion This review highlights some of the efforts that have been made in the appraisal of γ-PGA and its nanoparticles for drug delivery. γ-PGA is a candidate for future extensive use in drug delivery.

Characterization of Beldi chicken and turkeys in rural poultry flocks of Morocco. Current state and future outlook

2001 ◽  
Vol 31 ◽  
pp. 87-95 ◽  
Author(s):  
K. Benabdeljelil ◽  
T. Arfaoui
Keyword(s):  
Rural Areas ◽  
Sexual Maturity ◽  
Phenotypic Variability ◽  
Future Outlook ◽  
Current State ◽  
Production Improvement ◽  
Rural Poultry

SummaryThe main characteristics of Beldi (meaning “native” in Arabic) poultry raised in rural areas were studied in the Khenifra Region through extensive surveys.Beldi chickens have large phenotypic variability. Black, brown, grey and white colours, pure or mixed, are frequently observed in chickens' featherings while turkeys are predominantly bronze. Plumage types were fairly homogeneous in both species. Single combs and dented combs were respectively predominant in chicken hens and roosters.Sexual maturity was reached at 154 days for roosters, 168 for hens in chicken, around 217 days for toms and 231 for turkey hens. The age at first egg averaged 5.8 months for hens and 8.4 months for turkey hens. The number of eggs laid per hen per year was 78 for chicken and 69 for turkey. Hatchability rate was 78 and 80 percent for chicken and turkeys, respectively. Diseases were the main cause of mortality, which could affect up to 77 percent of chicken flocks.Few preliminary urgent steps for the conservation of Beldi poultry are discussed with other production improvement proposals.

Developments in Rechargeable Mno2Electrodes for Lithium Batteries

1988 ◽  
Vol 135 ◽  
Author(s):  
Michael M Thackeray
Keyword(s):  
Lithium Batteries ◽  
Lithium Battery ◽  
Electrode Materials ◽  
State Of The Art ◽  
Structural Features ◽  
Rechargeable Batteries ◽  
Current State ◽  
Alternative Systems ◽  
Battery Technology ◽  
Made In

AbstractConsiderable efforts are in progress to develop rechargeable batteries as alternative systems to the nickel-cadmium battery. In this regard, several advances have been made in ambient-temperature lithium battery technology, and specifically in the engineering of rechargeable lithium/manganese dioxide cells. This paper reviews the current state of the art in rechargeable Li/MnO2battery technology; particular attention is paid to the structural features of various MnO2electrode materials which influence their electrochemical and cycling behaviour in lithium cells.

Sign in / Sign up

Export Citation Format

Share Document