Serum levels of 9α,11β-PGF2
 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis

Abstract Chronic anemia of cancer can be corrected in approximately 50% of the cases by treatment with recombinant human erythropoietin (rHuEPO). Early prediction of responsiveness would avoid the emotional and financial burden of ineffective medical intervention. Eighty patients with chronic anemia of cancer undergoing treatment with rHuEPO (150 U/kg, 3 times per week by subcutaneous injection; after 6 weeks without response, 300 U/kg) participated in this study. Response was defined as a gain of at least 2 g/dL hemoglobin (Hb) within 12 weeks. Multivariate discriminant analysis and logistic regression analysis of response were performed on routine blood tests; serum levels of EPO, iron, ferritin, transferrin, and its receptor; World Health Organization (WHO) performance status; various cytokines; neopterin; stem cell factor; C- reactive protein; and alpha 1-antitrypsin. At baseline, none of these factors showed sufficient prognostic power. The following predictive algorithm was developed: (1) If after 2 weeks of therapy both the serum EPO level is > or = 100 mU/mL and Hb concentration has not increased by at least 0.5 g/dL, unresponsiveness of the patient is very likely (predictive power, 93%); otherwise, response may be predicted with an accuracy of 80%. (2) If both the serum level of EPO is less than 100 mU/mL and Hb concentration has increased by > or = 0.5 g/dL, response is highly probable (predictive power, 95%). (3) Alternatively, a serum ferritin level of > or = 400 ng/mL after 2 weeks of rHuEPO therapy strongly indicates unresponsiveness (predictive power, 88%), whereas a level less than 400 ng/mL suggests response in 3 of 4 patients.

Download Full-text

Predicting Liver Disease Risk Using a Combination of Common Clinical Markers: A Screening Model from Routine Health Check-Up

Disease Markers ◽

10.1155/2020/8460883 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Yi Wang ◽

Yanni Li ◽

Xiaoyi Wang ◽

Ranko Gacesa ◽

Jie Zhang ◽

...

Keyword(s):

Liver Disease ◽

Early Detection ◽

Predictive Power ◽

Disease Screening ◽

Health Check ◽

Initial Presentation ◽

Screening Tools ◽

Clinical Markers ◽

Liver Disorders ◽

High Predictive Power

Background. Early detection is crucial for the prognosis of patients with autoimmune liver disease (AILD). Due to the relatively low incidence, developing screening tools for AILD remain a challenge. Aims. To analyze clinical characteristics of AILD patients at initial presentation and identify clinical markers, which could be useful for disease screening and early detection. Methods. We performed observational retrospective study and analyzed 581 AILD patients who were hospitalized in the gastroenterology department and 1000 healthy controls who were collected from health management center. Baseline characteristics at initial presentation were used to build regression models. The model was validated on an independent cohort of 56 patients with AILD and 100 patients with other liver disorders. Results. Asymptomatic AILD individuals identified by the health check-up are increased yearly (from 31.6% to 68.0%, p<0.001). The cirrhotic rates at an initial presentation are decreased in the past 18 years (from 52.6% to 20.0%, p<0.001). Eight indicators, which are common in the health check-up, are independent risk factors of AILD. Among them, abdominal lymph node enlargement (LN) positive is the most significant different (OR 8.85, 95% CI 2.73-28.69, p<0.001). The combination of these indicators shows high predictive power (AUC=0.98, sensitivity 89.0% and specificity 96.4%) for disease screening. Except two liver or cholangetic injury makers, the combination of AGE, GENDER, GLB, LN, concomitant extrahepatic autoimmune diseases, and familial history also shows a high predictive power for AILD in other liver disorders (AUC=0.91). Conclusion. Screening for AILD with described parameters can detect AILD in routine health check-up early, effectively and economically. Eight variables in routine health check-up are associated with AILD and the combination of them shows good ability of identifying high-risk individuals.

Download Full-text

Prediction of Kindergarteners' Behavior on Metropolitan Readiness Tests from Preschool Perceptual and Perceptual-Motor Performances: A Validation Study

Perceptual and Motor Skills ◽

10.2466/pms.1981.52.3.899 ◽

1981 ◽

Vol 52 (3) ◽

pp. 899-902 ◽

Cited By ~ 1

Author(s):

David E. Belka

Keyword(s):

Multiple Regression ◽

Cognitive Performance ◽

Validation Study ◽

Auditory Perception ◽

Predictive Power ◽

Cognitive Achievement ◽

Regression Equations ◽

Motor Tasks ◽

High Predictive Power ◽

Motor Tests

Multiple regression equations were generated to predict cognitive achievement for 40 children (ages 57 to 68 mo.) 1 yr. after administration of a battery of 6 perceptual and perceptual-motor tests to determine if previous results from Toledo could be replicated. Regression equations generated from maximum R2 improvement techniques indicated that performance at prekindergarten is useful for prediction of cognitive performance (total score and total score without the copying subtest on the Metropolitan Readiness Tests) 1 yr. later at the end of kindergarten. The optimal battery included scores on auditory perception, fine perceptual-motor, and gross perceptual-motor tasks. The moderate predictive power of the equations obtained was compared with high predictive power generated in the Toledo study.

Download Full-text

Combined markers of initial stages of coronary atherosclerosis

Biomeditsinskaya Khimiya ◽

10.18097/pbmc20176303272 ◽

2017 ◽

Vol 63 (3) ◽

pp. 272-277 ◽

Cited By ~ 3

Author(s):

N.G. Gumanova ◽

M.V. Klimushina ◽

N.E. Gavrilova ◽

V.A. Metelskaya

Keyword(s):

Heart Disease ◽

Diagnostic Tests ◽

Coronary Atherosclerosis ◽

Predictive Power ◽

Serum Levels ◽

Functional Markers ◽

Gensini Score ◽

Male And Female ◽

Male Patients ◽

Coronary Lesions

Abnormalities in energy metabolism and endothelial dysfunction contribute to signaling processes associated with atherogenesis. The goal of our study was to develop diagnostic tests based on endothelial functional markers and adiponectin to differentiate early stages of coronary lesions during atherogenesis. The cohort included male and female patients from 25 to 86 years of age. All subjects underwent coronary angiography and severity of coronary lesions was quantified by the Gensini score that assigns points depending on location and extent of the lesions. We have estimated associations between the Gensini score and some known primary and secondary diagnostic parameters and have found that the ratio of serum levels of adiponectin to endothelin strongly correlates with severity of coronary lesions and can be used for differentiation of male patients lacking coronary atherosclerosis (despite symptoms of ischemic heart disease) from patients that have severe coronary lesions. Predictive power of adiponectin to endothelin ratio did not depend on drug therapy.

Download Full-text

Exploration of candidate serum biomarkers potentially related to the chronic pain condition in Medication Overuse Headache (MOH)

10.21203/rs.2.9106/v1 ◽

2019 ◽

Author(s):

Lanfranco Pellesi ◽

Elisa Bellei ◽

Simona Guerzoni ◽

Maria Michela Cainazzo ◽

Carlo Baraldi ◽

...

Keyword(s):

Neuropathic Pain ◽

Correlation Coefficient ◽

Trigeminal Nerve ◽

Chronic Headache ◽

Medication Overuse ◽

Medication Overuse Headache ◽

Serum Levels ◽

Apolipoprotein A1 ◽

Serum Concentrations ◽

Healthy Individuals

Abstract Background Medication Overuse Headache (MOH) is a prevalent and disabling disorder resulting from the overuse of analgesic drugs, triptans or other acute headache medications. In previous proteomic studies, several proteins have been found at high concentrations in the urine of MOH patients and in the serum of rats with neuropathic pain. The aim of this study was to compare the serum levels of lipocalin-type Prostaglandin D2 synthase (L-PGDS), Vitamin D-binding protein (VDBP), apolipoprotein E (APOE) and apolipoprotein A1 (APOA1) in MOH patients and healthy individuals, further exploring their relationship with cutaneous pain thresholds (CPTs) in the territories innervated by the trigeminal nerve. Methods 69 MOH patients and 42 age- and sex-matched healthy volunteers were enrolled in the study. Von Frey-like filaments were applied to the skin territories innervated by the trigeminal nerve, to determine the CPTs. L-PGDS, VDBP, APOE and APOA1 were quantified in the serum by Enzyme-linked Immunosorbent Assay (ELISA). Clinical and laboratory data were collected. Comparisons between MOH patients and healthy individuals were performed using independent t test or χ2 test. To correlate serum proteins with CPTs, Pearson correlation coefficient or Spearman's rank correlation coefficient were used. Results CPTs were lower among MOH patients. L-PGDS, VDBP and APOE had significantly different serum concentrations between groups (p < 0.01), but no correlation was found with CPTs. APOA1 serum concentrations did not differ between patients and healthy individuals. Conclusions L-PGDS, VDBP and APOE had abnormal serum levels in MOH patients, confirming their alteration in some conditions of chronic headache and neuropathic pain. The in-depth study of target proteins represents a promising approach for a better understanding of MOH, as well as the detection of candidate biomarkers for chronic headache or the risks associated with overuse medications.

Download Full-text

The identification of a potential prognostic model of colon cancer using integrated bioinformatics analysis

10.21203/rs.3.rs-39410/v1 ◽

2020 ◽

Author(s):

Zhengyu Fang ◽

Sumei Xu ◽

Yiwen Xie ◽

Wenxi Yan

Keyword(s):

Gene Expression ◽

Colon Cancer ◽

Prognostic Model ◽

Predictive Power ◽

Gene Signature ◽

Gene Expression Omnibus ◽

The Cancer Genome Atlas ◽

Cancer Prognosis ◽

Training Dataset ◽

High Predictive Power

Abstract Background This study aimed to construct prognostic model by screening prognostic gene signature of colon cancer. Methods The gene expression profile data of colon cancer were obtained from The Cancer Genome Atlas (TCGA) and gene expression omnibus (GEO) and differently expressed genes (DEGs) between tumor and control samples were identified. Prognosis-associated genes were then identified and used for the construction of prognostic model. The independent factors that associated with the prognosis of colon in the TCGA cohort was identified. Results Totally, 1153 consistent DEGs were screened out between tumor and normal tissues in the TCGA cohort, GSE44861 and GSE44076 datasets. Among these genes, 12 DEGs were related to the prognosis of colon cancer and were used for constructing the prognostic model. This model presented a high predictive power for the prognosis of colon cancer both in the training dataset and in the validation datasets (AUC > 0.8). Statistical analysis showed that age, pathological T, tumor recurrence, and model status were the independent factors for prognosis of patients with colon cancer in TCGA. Conclusions The 12-gene signature prognostic model had a high predictive power for colon cancer prognosis.

Download Full-text

CILP1 as a biomarker for right ventricular maladaptation in pulmonary hypertension

European Respiratory Journal ◽

10.1183/13993003.01192-2019 ◽

2020 ◽

pp. 1901192

Author(s):

Stanislav Keranov ◽

Oliver Dörr ◽

Leili Jafari ◽

Christian Troidl ◽

Christoph Liebetrau ◽

...

Keyword(s):

Pulmonary Hypertension ◽

Protein Expression ◽

Right Ventricular ◽

Predictive Power ◽

Pressure Overload ◽

Serum Levels ◽

Left Ventricular ◽

Dilative Cardiomyopathy ◽

Significant Difference ◽

Rv Function

The aim of our study was to analyse the protein expression of cartilage intermediate layer protein 1 (CILP1) in a mouse model of right ventricular (RV) pressure overload and to evaluate CILP1 as a biomarker of cardiac remodelling and maladaptive RV function in patients with pulmonary hypertension (PH).Pulmonary artery banding was performed in 14 mice; another 9 mice underwent sham surgery. CILP1 protein expression was analysed in all hearts by western blotting and immunostaining. CILP1 serum concentrations were measured in 161 patients (97 with adaptive and maladaptive RV pressure overload caused by PH; 25 with left ventricular (LV) hypertrophy; 20 with dilative cardiomyopathy (DCM); 19 controls without LV or RV abnormalities)In mice, the amount of RV CILP1 was markedly higher after banding than after sham. Control patients had lower CILP1 serum levels than all other groups (p<0.001). CILP1 concentrations were higher in PH patients with maladaptive RV function than those with adaptive RV function (p<0.001), LV pressure overload (p<0.001), and DCM (p=0.003). CILP1 showed good predictive power for maladaptive RV in ROC analysis (AUC 0.79). There was no significant difference between the AUCs of CILP1 and NT-pro-BNP (AUC 0.82). High CILP1 (≥cut-off value for maladaptive RV of 4373 pg·mL−1) was associated with lower TAPSE/PASP ratios (p<0.001) and higher NT-pro-BNP levels (p<0.001).CILP1 is a novel biomarker of RV and LV pathological remodelling that is associated with RV maladaptation and ventriculoarterial uncoupling in patients with PH.

Download Full-text

Proprotein convertase subtilisin/kexin type 9 in the dyslipidaemia of patients with axial spondyloarthritis is related to disease activity

Rheumatology ◽

10.1093/rheumatology/keaa590 ◽

2020 ◽

Author(s):

Laura de Armas-Rillo ◽

Juan C Quevedo-Abeledo ◽

Antonia de Vera-González ◽

Alejandra González-Delgado ◽

José A García-Dopico ◽

...

Keyword(s):

Disease Activity ◽

Lipid Profile ◽

Cholesterol Metabolism ◽

Multivariable Analysis ◽

Density Lipoprotein ◽

Intima Media Thickness ◽

Serum Levels ◽

Apolipoprotein A1 ◽

Proprotein Convertase ◽

Cross Sectional

Abstract Objective Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a serine protease that regulates cholesterol metabolism and has been linked to cardiovascular (CV) risk. The purpose of the present study was to examine whether PCSK9 levels are related to abnormalities in the lipid profile and the development of atherosclerosis that occurs in patients with axial SpA (axSpA). Methods We performed a cross-sectional study that encompassed 545 individuals; 299 patients with axSpA and 246 statin use–matched controls. PCSK9 and standard lipid profiles were analysed in patients and controls. Carotid intima-media thickness (cIMT) and carotid plaques were assessed in patients. A multivariable analysis, adjusted for standard CV risk factors, was performed to evaluate the influence of PCSK9 on axSpA-related dyslipidaemia and subclinical carotid atherosclerosis. Results Total cholesterol, high-density lipoprotein and low density lipoprotein cholesterol, lipoprotein (a) and apolipoprotein A1 were significantly lower in axSpA patients than controls. PCSK9 serum levels [β coefficient −44 ng/dl (95% CI −60, −27), P = 0.000] were also downregulated in axSpA patients after fully multivariable adjustment. ASDAS-CRP was found to be independently and significantly related to PCSK9 [β coefficient 10 ng/dl (95% CI 1, 18), P = 0.023] after analysing fully adjusted models that took age, sex and the rest of the lipid profile molecules into account. Whereas patients taking prednisone showed higher serum levels of PCSK9 [55 ng/ml (95% CI 24, 8), P = 0.001], those under anti-TNF-α therapies exhibited lower levels [β coefficient −26 ng/ml (95% CI −43, −9], P = 0.003]. Conclusion PCSK9 is downregulated in patients with axSpA. Disease activity is positive and significantly related to PSCK9. Anti-TNF-therapy yields a reduction in PCSK9 serum levels.

Download Full-text

ASSOCIATION OF LIPID FRACTIONS LEVELS WITH CARDIOVASCULAR DISEASE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i3.15984 ◽

2017 ◽

Vol 10 (3) ◽

pp. 180

Author(s):

Feryal H. Rada

Keyword(s):

Cardiovascular Disease ◽

Angina Pectoris ◽

Serum Lipids ◽

Case Control Study ◽

Serum Levels ◽

Apolipoprotein A1 ◽

Lipoprotein A ◽

Lipid Fractions ◽

Lipids And Lipoproteins ◽

Control Study

ABSTRACTObjective: The aim of this study is to analyze the association of serum lipids and lipoproteins in patients with angina pectoris.Methods: A total of 110 patients (60 males and 50 females) with angina pectoris aged (55±5) years and 80 healthy controls (43 males and37 females) aged (45±4) years were enrolled in this case-control study from the clinic of Al Yarmouk Hospital. Serum lipids, lipoprotein(a) [Lp(a)],apolipoprotein-A1, and apolipoprotein-B levels were measured and studied.Results: The results of this study showed that increased odds of angina pectoris were associated with increased serum levels of Lp(a) more thanserum levels of apolipoprotein-B.Conclusions: Analysis of Lp(a) may be an important determinant of cardiovascular disease diagnosis.Keywords: Lipoprotein(a), Apolipoprotein-A1, Apo lipoprotein-B, Angina pectoris.

Download Full-text

Comparison of Serum Apolipoprotein Levels of Diabetic Children and Healthy Children with or without Diabetic Parents

Cholesterol ◽

10.1155/2012/490381 ◽

2012 ◽

Vol 2012 ◽

pp. 1-4 ◽

Cited By ~ 3

Author(s):

Mohammad Hashemi ◽

Mohammad Saadat ◽

Mohaddeseh Behjati ◽

Roya Kelishadi

Keyword(s):

Type I Diabetes ◽

Serum Levels ◽

High Plasma ◽

Apolipoprotein A1 ◽

Type I ◽

Healthy Children ◽

Diabetic Children ◽

The Mean ◽

The Difference ◽

Lipids And Lipoproteins

Introduction. The association of diabetes and atherosclerosis with disorders of lipids and lipoproteins, notably high apolipoprotein B (apoB) and low apolipoprotein A1(apoA1) is well established. Because of the beginning of the atherosclerosis' process from early life, in this study, the plasma levels of apoA1 and apoB were compared in diabetic children with type I diabetes mellitus(DM), healthy children with diabetic parents (HDPs),and healthy children with nondiabetic parents (HNDPs). Methods. This case-control study was conducted among 90 children aged 9–18 years. Serum levels of apoA and apoB were compared among 30 diabetic children (DM), 30 healthy children with diabetic parents (HDPs), and 30 healthy children with nondiabetic parents (HNDP). Results. The mean serum apoA1 was higher in DM (153±69 mg/dL) followed by HNDPs (138±58 mg/dL) and HDPs (128±56 mg/dl), but the difference was not statistically significant. The mean apoB value in HNDPs was significantly lower than DM and HDPs (90±21 mg/dL versus 127±47 and 128±38 mg/dL, P<0.05, respectively). The mean apoB levels in DM (127±47 mg/dl) and HDP (128±38 mg/dL) were not statistically significantly different (P>0.05). Conclusions. Diabetic children and healthy children with diabetic parent(s) are at higher risk of dyslipidemia and atherosclerosis. Thus for primordial and primary prevention of atherosclerosis, we suggest screening these children for low plasma apoA1 and high plasma apoB levels.

Download Full-text