A novel phage indirectly regulates diatom growth by infecting diatom-associated biofilm-forming bacterium

Author(s):  
Shailesh Nair ◽  
Chengcheng Li ◽  
Shanli Mou ◽  
Zenghu Zhang ◽  
Yongyu Zhang

Algae and heterotrophic bacteria have close and intricate interactions, which are regulated by multiple factors in the natural environment. Phages are the major factor determining bacterial mortality. However, their impacts on the algae-associated bacteria and thus on the algae-bacteria interactions are poorly understood. Here, we obtained a diatom-associated bacterium Stappia indica SNL01 that could form biofilm and had an inhibitory effect on the growth of diatom Thalassiosira pseudonana . Meanwhile, the phage SI01 with a double-stranded circular DNA genome (44,247 bp) infecting S. indica SNL01 was isolated. Phylogenetic analysis revealed that phage SI01 represents a novel member of the Podoviridae family. The phage contained multiple lysis genes encoding for cell wall lysing muramidase and spore cortex lysing SleB, as well as depolymerase-like tail spike protein. By lysing the host bacterium and inhibiting the formation of biofilm, this phage could indirectly promote the growth of the diatom. Our results shed new insights into how phages indirectly regulate algal growth by infecting bacteria closely associated with algae or in the phycosphere. IMPORTANCE The impact of phage infection on the algae-bacteria relationship in the ocean is poorly understood. Here, a novel phage infecting the diatom-associated bacterium Stappia indica SNL01 was isolated. This bacterium could form biofilm and had a negative effect on diatom growth. We revealed that this phage contained multiple lysis genes and could inhibit the formation of bacterial biofilm, thus indirectly promoting diatom growth. This study implicates that phages are not only important regulators of bacteria but also have substantial indirect effects on algae as well as the algae-bacteria relationship.

2021 ◽  
Author(s):  
Shuhong Wang ◽  
Hanxue Chen ◽  
Kedong Yin

Abstract The employment effect of enterprises embedded in global value chains has important theoretical value, but existing research has ignored the impact of cross-border pollution transfer on employment under the division of labor system within the value chain. This study constructs a global environmental value chain (GEVC) analysis framework to combine economic and environmental issues and establishes a theoretical model to discuss the impact of the degree of enterprise embeddedness in the GEVC on employment. Using 2000–2006 data from the China Industry Business Performance and China Customs databases, the study finds that an increase in the degree of enterprise embeddedness has a significant inhibitory effect on employment, especially for female laborers, lower-skilled laborers, state-owned enterprises, private enterprises, and enterprises in the eastern region. The research also shows that the cost increase effect enhances the negative effect of increased GEVC embeddedness on employment, while the innovation promotion effect and the foreign direct investment effect serve to mitigate the negative effect. The results provide a reference for developing countries seeking to effectively protect people's livelihood and employment while achieving a leap in the division of labor along the green value chain.


2021 ◽  
Vol 13 (8) ◽  
pp. 4134
Author(s):  
Fang Yang ◽  
Qinfan Gan

Based on the perspective of regional differences and decentralization, this article investigated the impact of environmental regulations on Taiwanese investment in mainland China from theoretical and empirical perspectives, and analyzed whether local governments are competing to lower environmental standards to attract Taiwanese investment so as to maintain their comparative advantages. This paper constructed a theoretical model through a two-stage game model. With the panel data of each province in Mainland China from 2006 to 2016, the theoretical propositions were empirically tested through the system GMM estimation method. The results show that the environmental regulation policies adopted by the local governments in the mainland have a significant inhibitory effect on the investment volume of Taiwan-funded enterprises, and the interaction between environmental regulations and local tax burden levels also has a negative effect on Taiwanese investment. Local governments have the motive to reduce environmental regulations to attract investment.


2018 ◽  
Vol 10 (12) ◽  
pp. 4627 ◽  
Author(s):  
Shangmei Zhao ◽  
Jiang He ◽  
Haijun Yang

Using a panel of 31 Chinese provinces from 2000 to 2016, we investigated the impact of population ageing and financial deepening on economic growth. Based on the dynamic panel system GMM estimators, the empirical results address that both population ageing and financial deepening have a significantly positive impact on economic growth, while the interactions between them have a significantly negative effect on economic growth. From the perspective of total marginal effect, we also find that population ageing does contribute to economic growth but only when financial deepening is less than a threshold level; however, on the whole, financial deepening has an inhibitory effect on economic growth which increases with population ageing.


Author(s):  
Yingying Zhou ◽  
Yuehan Du ◽  
Fengyi Lei ◽  
Ziru Su ◽  
Yifei Feng ◽  
...  

In the wake of the acceleration of China’s industrialization and rapid economic growth, environmental pollution has also attracted great attention. The technological innovation of heavily polluting enterprises is conducive to reducing pollution emissions and promoting environmental health. The financial investment tendency and behavior of real enterprises have a significant impact on the technological innovation decision-making of enterprises. A panel model is used in this paper in order to empirically test the impact of financialization of Chinese heavily polluting enterprises on technological innovation based on the data of Listed Companies in Chinese heavily polluting industries from 2008 to 2019. The + results show that the financialization of heavily polluting enterprises has a significant crowding out effect on technological innovation. After introducing arbitrage motivation as the regulating variable, further research finds that arbitrage motivation weakens the inhibitory effect of enterprise financialization on technological innovation, that is, the stronger the arbitrage motivation, the smaller the negative effect of financialization on enterprise technological innovation, which weakens this crowding out effect. Finally, the listed enterprises in heavily polluting industries are divided into state-owned enterprises and non-state-owned enterprises according to their corporate attributes. Compared with state-owned enterprises, the financialization of non-state-owned enterprises has a greater squeeze out of technological innovation; and arbitrage motivation has a more significant regulatory effect on the impact of enterprise financialization on technological innovation.


2021 ◽  
Vol 937 (2) ◽  
pp. 022010
Author(s):  
M Klindukh ◽  
E Dobychina ◽  
M Makarov ◽  
I Ryzhik

Abstract Arctic ecosystems are the most sensitive and vulnerable to anthropogenic impact, namely, the influence of petroleum products. The aim is to identify the effect of diesel fuel (DF) on the composition of free amino acids (FAA) in Acrosiphonia arcta in Barents Sea. The impact of DF concentrations of 20 - 3000 maximal permitted concentration (MPC) was analyzed. It was found that A. arcta contains 20 amino acids, 16 of which are proteinogenic and 8 are essential. The composition of the FAA pool did not change under the influence of DF, but the content of individual FAA and their total amount differed. The ratio of the dominant amino acids changes: at concentrations of DF up to 1000 MPC content of proline increased, and decreased at higher concentrations of DF. Under the influence of DF in concentration of 3000 MPC, taurine content significant increased. The least negative effect was exerted under the influence of DF concentration of 20 MPC, more significant - at the concentration more than 1000 MPC. Changes in the content of FAA are probably associated with the redirection of the synthesis pathways of amino acid groups, as well as the inhibitory effect of the toxicant on protein synthesis.


2021 ◽  
Vol 13 (23) ◽  
pp. 13478
Author(s):  
Ping Wang ◽  
Hua Bu ◽  
Huaping Sun

Under certain circumstances, on-the-job consumption is conducive to improving the resilience of the supply chain and the sustainable development ability of enterprises. Using China’s A-share listed companies from 2008 to 2019 as sample data in conjunction with the deconstructive analysis of on-the-job consumption, we used the multiple linear regression model of econometrics to deeply analyze the impact of on-the-job consumption on the sustainable development of enterprises, test the regulatory effect of innovation efficiency, and explore the governance effect of internal control and anti-corruption. Research shows that reasonable and excessive on-the-job consumption have positive and inhibitory effects, respectively, on the sustainable development of enterprises and the relationship between innovation efficiency and the sustainable development of enterprises. Compared to private enterprises, the positive effect of reasonable on-the-job consumption is smaller in state-owned enterprises, while the negative effect of excessive on-the-job consumption is larger in state-owned enterprises. Further research suggests that internal control can weaken the inhibitory effect of excessive on-the-job consumption on the positive relationship between the innovation efficiency and the sustainable development of enterprises, and the weakening effect is even greater after the implementation of anti-corruption policies. Anti-corruption policies and internal control can form a complementary force, effectively restrain the agency effect of excessive on-the-job consumption, and promote the sustainable development of enterprises. This research not only expands the research perspective of on-the-job consumption but also adds new theoretical explanations and empirical evidence for how to achieve the sustainable development of enterprises.


Energies ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 3526 ◽  
Author(s):  
Linda Mezule ◽  
Ieva Berzina ◽  
Martins Strods

Fuel alcohol production yields can be influenced by lignocellulosic biomass loading. High solid loadings (>20 wt%) are suggested to have the potential to produce more products. However, most often, low substrate loadings (<5% solids, w/w) are used to ensure good wetting and enzyme accessibility, and to minimize any inhibitory effect on the hydrolysis products. Here, we analyzed the effect of substrate loading on the enzymatic hydrolysis of hay with non-commercial enzyme products obtained from white-rot fungi. A significant negative effect on hydrolysis was observed when 10 wt% hay loading was used with the commercial enzyme, however, non-commercial enzyme products from white-rot fungi had no impact on hydrolysis in biomass loading rates from 1 to 10 wt%. Moreover, it was estimated that enzymes extracted from white-rot fungi could be used at a concentration of 0.2 FPU/mL at a biomass loading from 1–10 wt%, resulting in 0.17–0.24 g of released reducing carbohydrates per gram of biomass. Higher concentrations did not result in any significant conversion increase. A mixing impact was only observed in test runs at a substrate loading of 10 wt%. The apparently positive features of the non-commercial enzyme mixes give rise to their future use. The combination and upgrade of existing technologies, e.g., efficient pre-treatment, membrane purification, and concentration and efficient product recovery, should result in even higher conversion yields.


2020 ◽  
Vol 6 (2) ◽  
pp. 145-173 ◽  
Author(s):  
Heidi E. Rademacher

Promoting the ratification of the Convention on the Elimination of All Forms of Discrimination against Women (CEDAW) was a key objective of the transnational women's movement of the 1980s and 1990s. Yet, few studies examine what factors contribute to ratification. The small body of literature on this topic comes from a world-society perspective, which suggests that CEDAW represented a global shift toward women's rights and that ratification increased as international NGOs proliferated. However, this framing fails to consider whether diffusion varies in a stratified world-system. I combine world-society and world-systems approaches, adding to the literature by examining the impact of women's and human rights transnational social movement organizations on CEDAW ratification at varied world-system positions. The findings illustrate the complex strengths and limitations of a global movement, with such organizations having a negative effect on ratification among core nations, a positive effect in the semiperiphery, and no effect among periphery nations. This suggests that the impact of mobilization was neither a universal application of global scripts nor simply representative of the broad domination of core nations, but a complex and diverse result of civil society actors embedded in a politically stratified world.


GIS Business ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. 85-98
Author(s):  
Idoko Peter

This research the impact of competitive quasi market on service delivery in Benue State University, Makurdi Nigeria. Both primary and secondary source of data and information were used for the study and questionnaire was used to extract information from the purposively selected respondents. The population for this study is one hundred and seventy three (173) administrative staff of Benue State University selected at random. The statistical tools employed was the classical ordinary least square (OLS) and the probability value of the estimates was used to tests hypotheses of the study. The result of the study indicates that a positive relationship exist between Competitive quasi marketing in Benue State University, Makurdi Nigeria (CQM) and Transparency in the service delivery (TRSP) and the relationship is statistically significant (p<0.05). Competitive quasi marketing (CQM) has a negative effect on Observe Competence in Benue State University, Makurdi Nigeria (OBCP) and the relationship is not statistically significant (p>0.05). Competitive quasi marketing (CQM) has a positive effect on Innovation in Benue State University, Makurdi Nigeria (INVO) and the relationship is statistically significant (p<0.05) and in line with a priori expectation. This means that a unit increases in Competitive quasi marketing (CQM) will result to a corresponding increase in innovation in Benue State University, Makurdi Nigeria (INVO) by a margin of 22.5%. It was concluded that government monopoly in the provision of certain types of services has greatly affected the quality of service experience in the institution. It was recommended among others that the stakeholders in the market has to be transparent so that the system will be productive to serve the society effectively


2019 ◽  
pp. 50-57
Author(s):  
Yu. V. Vertakova ◽  
A. S. Evtyukhin

The presented study addresses the issues of implementation of an import substitution policy in the Russian oil industry. Support of domestic production is a key objective specified in the message of the President of the Russian Federation in 2019. In light of the current economic situation and considering the importance of the oil industry for the Russian economy, an import substitution policy could help achieve the objective set by the President.Aim. The study aims to identify the problems and ways of implementation of an import substitution policy in the Russian oil industry.Tasks. The authors analyze scientific publications on import substitution and the state of the Russian oil industry at the current stage of development; determine the directions and fundamental trends in the development of the oil industry; identify obstacles that hinder the implementation of an import substitution policy in the oil industry; describe conditions that facilitate the development of the  investment process in the oil industry; propose comprehensive methods and ways to intensify investments and optimize costs for enterprises in the oil industry.Methods. This study uses general scientific methods of cognition, comparative and statistical analysis to identify problems in the implementation of an import substitution policy in the oil industry.Results. Examination of the current state and development prospects of the oil industry allows the authors to identify obstacles to the development of import substitution. The study shows their impact on the potential of import substitution, which has a negative effect on the prospects of intensification of investment processes, and proposes comprehensive methods and mechanisms that could neutralize the impact of the identified obstacles, thus facilitating the formation of a positive investment climate. Conclusions. Investment climate is shown to have a decisive impact on the potential of import substitution in the Russian oil industry, and ways of overcoming negative trends in the development of the oil industry are proposed.


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