Effects of fermented corn gluten meal on growth performance, serum parameters, intestinal morphology, and immunity performance of three-yellow broilers

2019 ◽  
Vol 99 (2) ◽  
pp. 408-417 ◽  
Author(s):  
Yan Wang ◽  
Xiaolan Liu ◽  
Long Jin ◽  
Qinan Wen ◽  
Yonggen Zhang ◽  
...  

A study was conducted to determine the effects of a diet containing solid-state fermented corn gluten meal (FCGM) with Bacillus natto and Lactobacillus on the growth performance, serum parameters, immune indices, and intestinal morphology of three-yellow broilers. A total of 450 one-d-old three-yellow male broilers were randomly allocated into three dietary treatment groups: control (without FCGM), T1, and T2 [5% and 10% replacement with corn gluten meal (CGM) and FCGM, respectively]. Ten replications per treatment and 15 birds per repeat were utilized. The results showed that the FCGM had a lower neutral detergent fiber content and higher small peptide content than the CGM. The average daily gain values, globulin content, the thymus and spleen indices of three-yellow broilers fed 5% and 10% FCGM were significantly higher (P < 0.05) than those of the control group, and the three-yellow broilers that were fed 10% FCGM increased more than those that were fed 5% FCGM (P < 0.05). The villus lengths and the ratios of the villus height to crypt depth (V:C) of the duodenum of three-yellow broilers fed 5% and 10% FCGM were significantly higher than those of the control group (P < 0.05). From this study, it can be concluded that the productivity performances of three-yellow broilers fed with 5% FCGM and 10% FCGM increased, and with the additive level increase in the diet, increases of the productivity performance were observed.

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bo Deng ◽  
Jie Wu ◽  
Xiaohui Li ◽  
Cheng Zhang ◽  
Xiaoming Men ◽  
...  

AbstractThe present study was conducted to investigate effects of Bacillus subtilis on growth performance, serum parameters, digestive enzymes, intestinal morphology, and colonic microbiota in piglets. A total of 72 piglets were weighed and randomly allotted into three treatments (four replication pens per treatment with six piglets/pen) for a 28-day experiment. The dietary treatments were as follows: basal diet (control group, CTR), basal diet supplementation with antibiotic (antibiotic group, ABT), and basal diet supplementation with 0.1% Bacillus subtilis (probiotic group, PBT). The average daily gain of body weight increased in both the ABT and PBT groups, and dietary antibiotics decreased the feed:gain ratio (F:G), as compared to the CTR group (P < 0.05). Both ABT and PBT piglets had increased serum triglycerides and lipase, amylase, maltase activities and villus height:crypt depth ratio (V/C) in ileum (P < 0.05). The PBT group also showed an increase in serum glucose and villus height in the ileum (P < 0.05). Dietary antibiotics increased Lactobacillus johnsonii, as compared to the CTR group, but decreased bacterial diversity and increased Escherichia coli, as compared to the PBT group (P < 0.05). Piglets dietary with B. subtilis modulated the microbiota by increasing the abundance of Firmicutes (L. johnsonii, L. reuteri) and decreasing the abundance of E. coli, as compared to the control group (P < 0.05). These results indicate that dietary of B. subtilis improves growth performance and intestinal health and can be a promising alternative to antibiotics in piglets diet.


2017 ◽  
Vol 15 (1) ◽  
pp. e06SC01 ◽  
Author(s):  
Saman Lashkari ◽  
Akbar Taghizadeh ◽  
Hamid Paya ◽  
Søren K. Jensen

The objective of the present study was to evaluate the effect of replacing corn with orange pulp (OP) on growth performance, rumen fermentation, nutrient digestibility and blood parameters of fattening lambs. Twenty male lambs were placed in individual pens and fed with four levels of replacement of corn by OP (0, 33, 66, 100%) during 60 days. Average daily gain (ADG) showed a quadratic effect (p<0.007) with the increasing levels of replacement. Inclusion of 33 and 66% of OP in the diet significantly increased dry matter intake (DMI) compared to control group (p<0.01). Ruminal ammonia-N concentration showed a linear decrease (p<0.002). Ruminal fluid pH increased linearly with the increasing replacement of corn by OP (p<0.001). Acetate concentration showed a linear increase (p<0.001). Plasma total protein showed a linear increase (p<0.002). Organic matter, crude protein and neutral detergent fiber showed a quadratic effect with the level of replacement. The results of the present study showed that replacement of corn by OP improves DMI of fattening lambs, leading to an enhancement in ADG at the replacement level of 40.3%. Also, total replacement of corn by OP did not have any adverse effect on growth performance, rumen fermentation, nutrient digestibility and blood parameters.


2021 ◽  
Vol 12 ◽  
Author(s):  
Simin Peng ◽  
Xin Wang ◽  
Yuyu Wang ◽  
Tuo Lv ◽  
Haohan Zhao ◽  
...  

Given the desirable results of using probiotics and enzyme preparations as feed supplements in poultry health, here, the effects of Bacillus and Non-starch Polysaccharase (NSPase) on the growth performance, serum antioxidant profiles, and gut microbial communities of early stage ducks is investigated. A total of 400 Zhijiang ducks (of similar body weight and 1 day age) was selected and randomly divided into four groups. The feeding period was 28 days. Each group contained 10 replicates of 10 birds. Control group (I) was fed with basal diet, while treatment groups II to IV were fed, respectively, with 150 mg/kg NSPases, 25 mg/kg Bacillus probiotics, and 150 mg/kg NSPases + 25 mg/kg Bacillus probiotics in their basal diet. The results demonstrated that dietary Bacillus (25 mg/kg) increased average final weight, average daily gain (ADG), and decreased the malonaldehyde (MDA) in birds (P &lt; 0.05). Dietary Bacillus (25 mg/kg) and NSPases + Bacillus (150 mg/kg + 25 mg/kg) presented much higher glutathione (GSH) and activities of superoxide dismutase (SOD) in birds (P &lt; 0.05). Additionally, as revealed by β-diversity indices and analysis of similarities, dietary NSPases + Bacillus could affect the ileum microbial abundances and diversities at the genera level (P &lt; 0.05), but it had no effect on the caecal microbiota. Also, 16S rRNA sequencing revealed that dietary Bacillus and NSPases + Bacillus increased the populations of Ruminococcaceae genera in the cecum (P &lt; 0.05), and S24-7_group and Lactobacillus genera in the ileum (P &lt; 0.05). However, dietary NSPases and Bacillus alone and in combination could significantly decrease the content of Bacteroides in the ileum (P &lt; 0.05). According to Spearman correlation analysis, 7 ilea bacterial microbiomes (S24-7 group, Lactobacillus, Subgroup 2, Subgroup 1, Kitasatospora, Candidatus Solibacter, and Akkermansia) were positively correlated with SOD (P &lt; 0.05). In conclusion, Bacillus (25 mg/kg) and NSPases (150 mg/kg) included in the diet could efficiently enhance the growth performance by altered gut microbiota composition at the genera level and antioxidant indices of ducks.


Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2428
Author(s):  
Jiao Chen ◽  
Xiaolin Niu ◽  
Fei Li ◽  
Fadi Li ◽  
Long Guo

(1) Background: we investigated the influence of dietary soybean meal (SBM) replaced with distillers dried grains with solubles (DDGS) plus rumen-protected (RP) lysine and methionine on the growth performance, nutrients digestion, rumen fermentation, and serum parameters of Hu sheep. (2) Methods: ninety Hu sheep were allocated to five groups: the control group (CON) which received the SBM diet, the DDGS group (NSM), the DDGS diet with RP lysine group (DRPL), the DDGS diet with RP methionine group (DRPM), and the DDGS diet with a mixture of RP lysine and methionine group (DRPLM). (3) Results: Final BW and carcass weight of the DRPLM and CON groups were greater (p ≤ 0.05) compared to NSM, DRPL, and DRPM groups. The DRPLM group tended to increase the dry matter intake (DMI, p = 0.06), average daily gain (ADG, p = 0.06), dressing percentage (p = 0.07), and tail fat weight (p = 0.09). The DRPLM group had increased (p ≤ 0.05) apparent digestibility and had altered ruminal fermentation characteristics. (4) Conclusions: replacement of SBM with DDGS in a diet with adequate metabolizable protein and by-pass amino acids (lysine and methionine) could maintain the growth performance of Hu sheep.


Author(s):  
Wen-Chao Liu ◽  
Yan Guo ◽  
Zhi-Hui Zhao ◽  
Rajesh Jha ◽  
Balamuralikrishnan Balasubramanian

This study aimed to determine the efficacy of dietary algae-derived polysaccharides (ADPs) from Enteromorpha on growth performance, intestinal morphology, intestinal permeability, and antioxidant capacity in serum, liver, and intestinal mucosa of broilers. Three hundred and ninety six day-old male chicks were randomly assigned to six dietary treatments containing 0 (Control), 1,000, 2,500, 4,000, 5,500, and 7,000 mg ADP/kg basal diet in a 35 day feeding trial. During day 1–21, compared with the control group, dietary 1,000–7,000 mg/kg ADP supplementation improved the average daily gain (ADG) and feed conversion ratio (p &lt; 0.05). Overall (day 1–35), dietary inclusion of 1,000 mg/kg ADP increased the final body weight and ADG (p &lt; 0.05). Besides, on day 21, dietary 2,500 mg/kg ADP supplementation increased the serum catalase (CAT) and liver total superoxide dismutase (T-SOD) activities (p &lt; 0.05), whereas dietary 1,000–5,500 mg/kg ADP supplementation decreased malondialdehyde (MDA) contents in serum and liver (p &lt; 0.05). On day 35, supplementation of 1,000 mg/kg ADP increased the serum glutathione peroxidase and CAT activities and liver T-SOD activities (p &lt; 0.05). It decreased the MDA level of serum and liver (p &lt; 0.05). Also, dietary 2,500 mg/kg ADP increased the villus height of jejunum and ileum on day 21 (p &lt; 0.05), and dietary 4,000 mg/kg ADP increased the villus height of duodenum and ileum on day 35 (p &lt; 0.05). On day 21, dietary 4,000 mg/kg ADP increased the CAT activities of the duodenum and T-SOD activities of jejunum and ileum and decreased the MDA contents in the duodenum, jejunum, and ileum (p &lt; 0.05). On day 35, dietary inclusion of 1,000–7,000 mg/kg ADP reduced MDA contents of duodenum and jejunum (p &lt; 0.05). Furthermore, dietary inclusion of ADP at 1,000–7,000 mg/kg decreased serum DAO activities at day 21 and day 35 (p &lt; 0.05), and the serum D-lactic acid concentration was reduced by dietary supplementation of 1,000, 2,500, and 7,000 mg/kg ADP on day 21. In conclusion, dietary ADP exerted beneficial effects on growth performance, antioxidant capacity, and gut health in broilers; based on the studied parameters, the appropriate recommended dose is 1,000–4,000 mg/kg. These findings provided new insights into the potential application of ADP as natural growth promoters in broilers.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 170-171
Author(s):  
Laura A Merriman ◽  
Gustavo Cordero ◽  
Pete Wilcock ◽  
Joaquin Morales

Abstract As an alternative to antibiotic growth promoters, live yeast supplementation has proven useful in reducing weaning stress and improving health and performance of piglets. The objective of the study was to evaluate the effect of a high concentrated live yeast product (20 billion CFU/g) supplemented in post-weaning piglet diets without antibiotics. A total of 144 pigs Pietrain × [LW×Landrace] (average initial BW = 8.55 ± 1.5 kg) weaned at 28 days of life were used for a 6-week growth study to determine post-weaning performance. Pigs were assigned to 2 treatments; 0 (Control group; CT) and 1 g of live yeast/kg (Yeast Supplemented group; YS) feed to 12 replicate pens of 6 pigs (3 barrows; 3 gilts). Average daily gain (ADG), feed intake (ADFI) and feed conversion ratio (FCR) were evaluated in pre-starter (days 0–14), starter (days 14–42) and in the whole nursery (days 0–42) periods. Data were analysed with ANOVA using PROC MIXED of SAS according to the completely randomized design. The model included initial BW as a covariant. Differences were deemed statistically significant when P ≤ 0.050 using a one tailed t-test. Live yeast supplementation improved ADG in starter (5.8%; P = 0.034) and in the whole nursery periods (7.0%; P = 0.045) compared with the CT group. Consequently, final BW was also higher in supplemented animals than in the CT (4.5%; P = 0.034). Differences in ADG were associated with differences in ADFI, higher in YS than in the CT group, both in starter (6.4%; P = 0.023) and in the whole nursery period (6.5%; P = 0.030). No differences were observed for FCR, mortality rate, and health incidences between dietary treatment groups. Therefore, supplementation of a live yeast to post-weaned piglets improved growth performance, confirming this product as an alternative to reduce antibiotic usage in nursery piglets.


2021 ◽  
Vol 99 (2) ◽  
Author(s):  
Mariano C Parra ◽  
Diogo F A Costa ◽  
Andre S V Palma ◽  
Karine D V Camargo ◽  
Lais O Lima ◽  
...  

Abstract The objective was to evaluate the effects of a specific strain of live yeast (LY) on growth performance, fermentation parameters, feed efficiency, and bacterial communities in the rumen of growing cattle fed low-quality hay. In experiment (exp.) 1, 12 Droughtmaster bull calves (270 ± 7.6 kg initial body weight [BW]) were blocked by BW into two groups, allocated individually in pens, and fed ad libitum Rhodes grass hay (8.4% of crude protein [CP]) and 300 g/bull of supplement (52% CP) without (Control) or with LY (8 × 109 colony-forming unit [CFU]/d Saccharomyces cerevisiae CNCM I-1077; Lallemand Inc., Montreal, Canada) for 28 d, followed by 7 d in metabolism crates. Blood and rumen fluid were collected before feeding and 4 h after feeding. In exp. 2, for assessment of growth performance, 48 Charbray steers (329 ± 20.2 kg initial BW) were separated into two blocks by initial BW and randomly allocated into 12 pens. The steers were fed Rhodes grass hay (7.3% CP) and 220 g/steer of supplement (60% CP) without or with LY (8 × 109 CFU/d) for 42 d, after a 2-wk adaptation period. In exp. 1, fiber digestibility was calculated from total fecal collection, and, in exp 2, indigestible neutral detergent fiber (NDF) was used as a marker. Inclusion of LY increased (P = 0.03) NDF intake by 8.3% in exp. 1, without affecting total tract digestibility. No changes were observed in microbial yield or in the efficiency of microbial production. There was a Treatment × Time interaction (P &lt; 0.01) for the molar proportion of short-chain fatty acids, with LY increasing propionate before feeding. Inclusion of LY decreased rumen ammonia 4 h after feeding (P = 0.03). The addition of LY reduced rumen bacterial diversity and the intraday variation in bacterial populations. Relative populations of Firmicutes and Verrucomicrobia varied over time (P &lt; 0.05) only within the Control group. At the genus level, the relative abundance of an unclassified bacterial genus within the order Clostridiales, a group of cellulolytic bacteria, was reduced from 0 to 4 h after feeding in the Control group (P = 0.02) but not in the LY group (P = 1.00). During exp. 2, LY tended to increase average daily gain (ADG) (P = 0.08) and feed efficiency (P = 0.10), with no effect on NDF intake or digestibility. In conclusion, S. cerevisiae CNCM I-1077 reduced the intraday variation of rumen bacteria and increased the amount of NDF digested per day. These observations could be associated with the tendency of increased ADG and feed efficiency in growing cattle fed a low-quality forage.


2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Simeng Liao ◽  
Shengguo Tang ◽  
Bie Tan ◽  
Jianjun Li ◽  
Ming Qi ◽  
...  

We investigated the effects of rapamycin (RAPA) and chloroquine (CQ) in supporting growth performance and the intestinal mucosal barrier in response to deoxynivalenol (DON) in piglets. A total of 32 healthy weaned piglets (bodyweight 7.10±0.58 kg) were divided into four groups and treated daily with RAPA (1 mg/kg BW), CQ (10 mg/kg BW), or a control volume of normal saline (two groups) until the end of the experiment. After feeding a basal diet for seven days, three groups were then switched to mildewed feed containing 1 mg kg/DON for a further seven days. In contrast to the control group, DON-treated piglets showed decreased average daily gain (ADG) and daily feed intake (ADFI), as well as negatively affected intestinal morphology as indicated by villus height, crypt depth, and tight junction protein expression. A group treated with RAPA and DON showed increased intestinal autophagy, aggravated inflammatory responses, and damage to the intestinal mucosa and permeability, leading to reduced growth performance. Meanwhile, a group treated with CQ and DON showed indices comparable to the non-DON control group, with alleviated inflammatory cytokines and healthy intestinal morphology and structure. They also showed better growth performance compared to DON treatment alone. These findings have important implications for mediating autophagy against DON in vivo, as well as the potential for CQ in improving growth performance and maintaining intestinal barrier integrity in weanling piglets.


Author(s):  
Xiaoping Zhu ◽  
Xiuguo Shang ◽  
Guozhao Lin ◽  
Haojie Li ◽  
Xin Feng ◽  
...  

Abstract The purpose of this study was to investigate the effects of zinc glycinate (Gly-Zn) on growth performance, serum biochemical index, intestinal morphology, and hepatic metallothionein (MT) mRNA expression in the liver of yellow feather broilers. A total of 540 18-day-old yellow feather broilers were randomly divided into three groups: control group (basal diet), ZnSO4 group (basal diet plus 60 mg Zn/kg from ZnSO4), and Gly-Zn group (basal diet plus 60 mg Zn/kg from zinc glycinate). Each treatment group had 6 replicates with 30 birds in each replicate. The experiment lasted for 42 days (18 to 59 days of age). The results showed that Gly-Zn supplementation significantly improved the average daily gain (ADG) and average daily feed intake (ADFI) of broilers during 18 to 39 days of age compared with that in the control group (P < 0.05) but not different from the ZnSO4 group. The Gly-Zn group had higher glutathione peroxidase (GSH-Px) (P < 0.05) and lower malondialdehyde (MDA) concentrations than the broilers in the control and ZnSO4 group. It was also observed that zinc content in the tibia of Gly-Zn group broilers was higher than the control and ZnSO4 group (P < 0.05). The results of intestinal morphology parameters showed that the Gly-Zn group significantly increased the villus height in duodenum and jejunum (P < 0.05) and decreased crypt depth in duodenum and ileum compared to the control group. However, there were no significant differences between the Gly-Zn group and ZnSO4 group in duodenum and ileum regarding intestinal morphology parameters. The Gly-Zn group significantly increased mRNA expression of MT in the liver than both control and ZnSO4 groups (P < 0.05). Collectively, the results indicated that supplementing 60 mg Zn/kg through zinc glycinate improved growth performance and serum indexes as well as intestinal morphology of yellow feather broilers. It also regulates MT gene expression more effectively than the ZnSO4 group at the transcriptional level.


Author(s):  
Recep Gümüş ◽  
Nazlı Ercan ◽  
Halit İmik

The aim of this study was to determine the effects on some serum parameters of wheat and corn gluten diet, which is also a high protein source. A total of 24 male rats aged 20 days and divided into 3 groups, each containing 8 rats were used in the study. The majority of the protein content in the diet of soybean meal as a Control group; in the diet of wheat gluten as a Wheat group; in the diet of corn gluten as a Corn group were composed. At the end of the study, blood samples taken from all animals were analysed. In the study, serum glucose, TP, urea, creatinine and BUN levels were found to be significantly decreased in Corn group compared to Wheat group. Serum ALT levels were similar with the Control and Wheat groups, but it was found to be significantly decreased in the Corn group. Serum AST levels were significantly lower in the Corn group. Serum GGT levels were significantly lower in both Corn and Wheat groups compared to Control group. Serum Ca and P levels were similar with all groups, whereas Mg levels were significantly decreased in Wheat and Corn groups compared to the Control group. As a result, it can be said that corn gluten added to the diet has more positive effects than wheat gluten in terms of both liver enzyme activities (AST, ALT and GGT) and renal parameters (Urea and BUN).


Sign in / Sign up

Export Citation Format

Share Document