Salinity tolerance of Gyrodactylus salaris (Platyhelminthes, Monogenea): laboratory studies

1997 ◽  
Vol 54 (8) ◽  
pp. 1837-1864 ◽  
Author(s):  
A Soleng ◽  
T A Bakke
Keyword(s):  
Population Growth ◽  
Survival Time ◽  
Fresh Water ◽  
Salinity Tolerance ◽  
Sea Water ◽  
Infected Fish ◽  
Laboratory Studies ◽  
Water Dispersal ◽  
Significant Difference ◽  
Parasite Survival

The salinity tolerance of the freshwater monogenean Gyrodactylus salaris, infecting Atlantic salmon (Salmo salar) parr, was studied experimentally. Following direct transfer of infected fish from fresh water to 5.0omicron salinity, parasite population growth increased at the same rate as in fresh water and was positively correlated with temperature (1.4, 6.0, and 12.0°C). In 7.5omicron salinity the populations declined and became extinct after a maximum of 56 days, without any significant difference between 6.0 and 12.0°C. However, some infrapopulations demonstrated short periods of growth. At higher salinities (10.0, 15.0, 20.0, and 33.0omicron) the survival time decreased, and there was a negative correlation between survival time and temperature (1.4, 6.0, and 12.0°C). When transferred directly to sea water (33.0omicron) the parasites became opaque and ceased moving after a few minutes. There was no difference in parasite survival time between direct and gradual transfer from fresh water to 7.5 and 10.0omicron, except for one infrapopulation which demonstrated population growth from day 22 after some fluctuations following gradual transfer to 7.5omicron. The present findings support the hypothesis of brackish water dispersal of G. salaris with infected fish migrating between rivers in fjord systems.

Oxygen Consumption of the Euryhaline Fish Aholehole (Kuhlia sandvicensis) with Reference to Salinity, Swimming, and Food Consumption

1972 ◽  
Vol 29 (1) ◽  
pp. 67-77 ◽  
Author(s):  
B. S. Muir ◽  
A. J. Niimi
Keyword(s):  
Body Weight ◽  
Oxygen Consumption ◽  
Fresh Water ◽  
Food Consumption ◽  
Sea Water ◽  
Swimming Activity ◽  
Daily Ration ◽  
Significant Difference ◽  
Fish Weight ◽  
The Difference

Active and standard metabolism of Kuhlia sandvicensis increase with fish weight to a power of about 0.8 and active is nine times standard. No significant difference was found between experiments in fresh water and 30‰ sea water at 23 C. At low swimming speeds the fish may be unable to physically take up as much oxygen as at higher speeds. Swimming activity may be essential to circulatory adequacy.Elevated oxygen consumption lasted for 42 hr following a ration of 2.3% of body weight and for 60 hr after one of 4.5%. It amounted to about 76 mg O2/g ration, equivalent to about 16% of the energy of the ration, in both cases. For a nonswimming fish the highest oxygen consumption observed following the maximum daily ration is no more than half of the difference between active and standard rates.

scholarly journals The Development of Salinity Tolerance in the Salmon, Salmo Salar (L.) and Some Related Species

1960 ◽  
Vol 37 (2) ◽  
pp. 425-434
Author(s):  
GWYNETH PARRY
Keyword(s):  
Fresh Water ◽  
Salmo Salar ◽  
Salinity Tolerance ◽  
Related Species ◽  
Sea Water ◽  
Osmotic Regulation ◽  
Salmonid Fishes ◽  
Survival Pattern

1. A study has been made of the survival and osmotic regulation of young salmonid fishes following transfer from fresh water to various dilutions of sea water 2. Survival is in the order: Salmo salar > S. gairdnerii > S. trutta and is generally better the larger the fish. 3. The survival pattern of alevins differs from that of the older stages. 4. Hypo-osmotic regulation is first seen in parr and becomes fully effective in smolts.

LUMINESCENCE OF THE BLACK SEA MICROSCOPIC FUNGI CULTURES

2017 ◽  
Author(s):  
Olga Mashukova ◽  
Olga Mashukova ◽  
Yuriy Tokarev ◽  
Yuriy Tokarev ◽  
Nadejda Kopytina ◽  
...  
Keyword(s):  
Fresh Water ◽  
Black Sea ◽  
Ethyl Alcohol ◽  
Light Emission ◽  
Sea Water ◽  
Control Culture ◽  
Chemical Stimulation ◽  
The Black Sea ◽  
The Given ◽  
First Time

We studied for the first time luminescence characteristics of the some micromycetes, isolated from the bottom sediments of the Black sea from the 27 m depth. Luminescence parameters were registered at laboratory complex “Svet” using mechanical and chemical stimulations. Fungi cultures of genera Acremonium, Aspergillus, Penicillium were isolated on ChDA medium which served as control. Culture of Penicillium commune gave no light emission with any kind of stimulation. Culture of Acremonium sp. has shown luminescence in the blue – green field of spectrum. Using chemical stimulation by fresh water we registered signals with luminescence energy (to 3.24 ± 0.11)•108 quantum•cm2 and duration up to 4.42 s, which 3 times exceeded analogous magnitudes in a group, stimulated by sea water (p < 0.05). Under chemical stimulation by ethyl alcohol fungi culture luminescence was not observed. Culture of Aspergillus fumigatus possessed the most expressed properties of luminescence. Stimulation by fresh water culture emission with energy of (3.35 ± 0.11)•108 quantum•cm2 and duration up to 4.96 s. Action of ethyl alcohol to culture also stimulated signals, but intensity of light emission was 3–4 times lower than under mechanical stimulation. For sure the given studies will permit not only to evaluate contribution of marine fungi into general bioluminescence of the sea, but as well to determine places of accumulation of opportunistic species in the sea.

Reosquido Desalinasi Metode Evaporasi dengan Ultraviolet Berbasis Mikrokontroller

2018 ◽  
Vol 3 (2) ◽  
pp. 38-47
Author(s):  
Muhammad Abdul Azis ◽  
Nuryake Fajaryati
Keyword(s):  
Temperature Measurement ◽  
Fresh Water ◽  
Sea Water ◽  
Evaporation Process ◽  
Heater Element ◽  
The Third ◽  
Turn On ◽  
Speed Up ◽  
Arduino Uno

This research aims to create a Reosquido desalination tool for evaporation methods using a microcontroller. This tool can control the temperature to speed up the evaporation process in producing fresh water. The method applied to Reosquido desalination uses Evaporation. The first process before evaporation is the detection of temperature in sea water that will be heated using an element heater. The second process of temperature measurement is to turn off and turn on the Arduino Uno controlled heater, when the temperature is less than 80 ° then the heater is on. The third process is evaporation during temperatures between 80 ° to 100 °, evaporation water sticks to the glass roof which is designed by pyramid. Evaporated water that flows into the reservoir is detected by its solubility TDS value. The fourth process is heater off when the temperature is more than 100 °. Based on the results of the testing, the desalination process using a microcontroller controlled heater can speed up the time up to 55% of the previous desalination process tool, namely manual desalination prsoes without using the heater element controlled by the temperature and controlled by a microcontroller which takes 9 hours. Produces fresh water as much as 30ml from 3000ml of sea water, so that it can be compared to 1: 100.

scholarly journals The Effectiveness of Candidate Probiotic Bacteria to Control Vibriosis Disease

2017 ◽  
Vol 5 (2) ◽  
pp. 1
Author(s):  
Mulyati Mulyati ◽  
Suryati Suryati ◽  
Irfani Baga
Keyword(s):  
Survival Rate ◽  
Sea Water ◽  
Challenge Test ◽  
Probiotic Bacteria ◽  
Pathogenicity Test ◽  
Inhibitory Ability ◽  
Significant Difference ◽  
Portunid Crab

The study aims to isolate, characterize, and examine probiotic bacteria's inhibitory ability against Vibrio harveyi bacteria, both in-vitro and in vivo. Methods used in the study consist of 1) An Isolation of Candidate Probiotic Bacteria, 2) An Antagonistic Test of Candidate Probiotic Bacteria in vitro, 3) An Identification of Bacteria, 4) A Pathogenicity Test of Candidate Probiotic Bacteria, 5) An Antagonistic Test of Candidate Probiotic Bacteria against V. harveyi in vivo. According to the isolation of candidate probiotic bacteria, there are 18 isolated candidate probiotic. After being tested for its inhibitory ability in vitro, there are 8 isolates with zone of inhibition as follows: isolate MM 7 from intestine (22 mm), isolate MM 6 from intestine (12 mm), isolate MM 10 from sea water (10 mm), isolate MM 5 from intestine (9 mm), isolate MM 4 from intestine (8 mm), isolate MM 3 from intestine (7 mm), isolate MM 2.2 from intestine (7 mm), isolate MM 2.1 from intestine (7 mm). Eight genera of the candidate probiotic bacteria is derived from Portunid crab, they are Staphylococcus, Streptococcus, bacillus, vibrio, Alcaligenes, Lactobacillus, micrococcus. Before proceeding the V. harveyi bacterial challenge test in vivo, three potential isolates consisting of MM6, MM7 and MM10 as the probiotic bacteria are pathogenicity-tested against V. harveyi. The survival rate of Portunid crab on pathogenicity test using MM6, MM7 and MM10 generates 91.11-100%, while the control generates 100% survival rate. Variance analysis result through post-hoc Tukey's Honest Significant Difference (HSD) test at 95% confidence interval indicates that isolate MM7 and MM10 are significantly able to increase hatchling Portunid crab's survival rate.

scholarly journals Cohort study of the overall survival of patients with pancreatic cancer in a hospital of specialties of Quito-Ecuador in the period 2007–2017

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Andrés Moreno Roca ◽  
Luciana Armijos Acurio ◽  
Ruth Jimbo Sotomayor ◽  
Carlos Céspedes Rivadeneira ◽  
Carlos Rosero Reyes ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cohort Study ◽  
Survival Time ◽  
Univariate Analysis ◽  
Rank Test ◽  
Kaplan Meier ◽  
Significant Difference ◽  
Icd 10 ◽  
The Difference ◽  
Clinical Records

Abstract Objectives Pancreatic cancers in most patients in Ecuador are diagnosed at an advanced stage of the disease, which is associated with lower survival. To determine the characteristics and global survival of pancreatic cancer patients in a social security hospital in Ecuador between 2007 and 2017. Methods A retrospective cohort study and a survival analysis were performed using all the available data in the electronic clinical records of patients with a diagnosis of pancreatic cancer in a Hospital of Specialties of Quito-Ecuador between 2007 and 2017. The included patients were those coded according to the ICD 10 between C25.0 and C25.9. Our univariate analysis calculated frequencies, measures of central tendency and dispersion. Through the Kaplan-Meier method we estimated the median time of survival and analyzed the difference in survival time among the different categories of our included variables. These differences were shown through the log rank test. Results A total of 357 patients diagnosed with pancreatic cancer between 2007 and 2017 were included in the study. More than two-thirds (69.9%) of the patients were diagnosed in late stages of the disease. The median survival time for all patients was of 4 months (P25: 2, P75: 8). Conclusions The statistically significant difference of survival time between types of treatment is the most relevant finding in this study, when comparing to all other types of treatments.

Long-Term Follow-up of Patients with Recurrent Malignant Gliomas Treated with Adjuvant Adoptive Immunotherapy

Neurosurgery ◽  
1991 ◽  
Vol 28 (1) ◽  
pp. 16-23 ◽  
Author(s):  
Kevin O. Lillehei ◽  
Dawn H. Mitchell ◽  
Stephen D. Johnson ◽  
Larry E. McCleary ◽  
Carol A. Kruse
Keyword(s):  
Survival Time ◽  
Adoptive Immunotherapy ◽  
Peripheral Blood ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Lak Cells ◽  
Prior Chemotherapy ◽  
Blood Lymphocytes ◽  
Significant Difference

Abstract Between August 1986 and October 1987, the Denver Brain Tumor Research Group conducted a clinical trial using autologous human recombinant interleukin-2 (rIL-2)-activated lymphocytes to treat 20 patients with recurrent high-grade gliomas. The trial involved surgical resection and/or decompression followed by intracavitary implantation of lymphokine-activated killer (LAK) cells and autologous stimulated lymphocytes (ASL) along with rIL-2 in a plasma clot. One month later, stimulated lymphocytes and rIL-2 were infused through a Rickham reservoir attached to a catheter directed into the tumor bed. The LAK cells were rIL-2-activated peripheral blood lymphocytes cultured for 4 days; the ASL were lectin- and rIL-2-activated peripheral blood lymphocytes cultured for 10 days. Of the 20 patients treated, 11 were evaluated as a group (mean age, 44 years, range, 15-61 years; mean Karnofsky rating, 69, range, 50-100; mean Decadron dose at entry, 14 mg/d. range, 0-32). The average number of lymphocytes implanted was 7.6 × 109 (range, 1.9-27.5 × 109), together with 1 to 4 × 106 U of rIL-2. To date, 10 of the 11 patients died, all from recurrent tumor growth. The median overall survival time was 63 weeks (range, 36-201; mean, 86). The median survival time after immunotherapy was 18 weeks (range, 11-151; mean, 39). No significant difference in survival after immunotherapy was found between those patients who had received previous chemotherapy and those who had not. The use of steroids or prior chemotherapy did not influence the in vitro generation of ASL or LAK cells. Prior chemotherapy did correlate, however, with diminished in vitro cytotoxicity against the natural killer-sensitive (K562) target cell by LAK cells (P &lt; 0.05) but not that by ASL. There were no major adverse side effects. Although adoptive immunotherapy was safe and well tolerated, its therapeutic potential remains in question.

Role of Apolipoprotein A-I in Protecting against Endotoxin Toxicity

2004 ◽  
Vol 36 (6) ◽  
pp. 419-424 ◽  
Author(s):  
Juan Ma ◽  
Xue-Ling Liao ◽  
Bin Lou ◽  
Man-Ping Wu
Keyword(s):  
Survival Time ◽  
Binding Capacity ◽  
Density Lipoprotein ◽  
Mtt Method ◽  
Apolipoprotein A ◽  
Plasma Fraction ◽  
Significant Difference ◽  
In Vivo Effects

Abstract High density lipoprotein (HDL) binds lipopolysaccharide (LPS or endotoxin) and neutralizes its toxicity. We investigated the function of Apolipoprotein A-I (ApoA-I), a major apolipoprotein in HDL, in this process. Mouse macrophages were incubated with LPS, LPS+ApoA-I, LPS+ApoA-I+LFF (lipoprotein-free plasma fraction d>1.210 g/ml), LPS+HDL, LPS+HDL+LFF, respectively. MTT method was used to detect the mortality of L-929 cells which were attacked by the release-out cytokines in LPS-activated macrophages. It was found that ApoA-I significantly decreased L-929 cells mortality caused by LPS treatment (LPS vs. LPS+ApoA-I, P<0.05) and this effect became even more significant when LFF was utilized (LPS vs. LPS+ApoA-I+LFF, P<0.01; LPS vs. LPS+HDL+LFF, P<0.01). There was no significant difference between LPS+ApoA-I+LFF and LPS+HDL+LFF treatment, indicating that ApoA-I was the main factor. We also investigated in vivo effects of ApoA-I on mouse mortality rate and survival time after LPS administration. We found that the mortality in LPS+ApoA-I group (20%) and in LPS+ApoA-I+LFF group (10%) was significantly lower than that in LPS group (80%) (P<0.05, P<0.01, respectively); the survival time was (43.20 ± 10.13) h in LPS+ApoA-I group and (46.80 ± 3.79) h in LPS+ApoA-I+LFF group, which were significantly longer than that in LPS group (16.25 ± 17.28) h (P<0.01). We also carried out in vitro binding study to investigate the binding capacity of ApoA-I and ApoA-I+LFF to fluorescence labeled LPS (FITC-LPS). It was shown that both ApoA-I and ApoA-I+LFF could bind with FITC-LPS, however, the binding capacity of ApoA-I+LFF to FITC-LPS (64.47 ± 8.06) was significantly higher than that of ApoA-I alone (24.35 ± 3.70) (P<0.01). The results suggest that: (1) ApoA-I has the ability to bind with and protect against LPS; (2) LFF enhances the effect of ApoA-I; (3) ApoA-I is the major contributor for HDL anti-endotoxin function.

Solar Water Distillation Using Two Different Phase Change Materials

2014 ◽  
Vol 592-594 ◽  
pp. 2409-2415 ◽  
Author(s):  
S. Naga Sarada ◽  
Banoth Hima Bindu ◽  
Sri Rama R. Devi ◽  
Ravi Gugulothu
Keyword(s):  
Solar Energy ◽  
Phase Change ◽  
Fresh Water ◽  
Alternative Energy ◽  
Potable Water ◽  
Sea Water ◽  
Underground Water ◽  
Solar Still ◽  
Water Distillation ◽  
The World

In recent years with the exacerbation of energy shortage, water crisis increases around the world. With the continuous increase in the level of greenhouse gas emissions, the use of various sources of renewable energy is increasingly becoming important for sustainable development. Due to the rising oil price and environmental regulations, the demand of utilizing alternative power sources increased dramatically. Alternative energy and its applications have been heavily studied for the last decade. Energy and water are essential for mankind that influences the socioeconomic development of any nation. Pure water resources become more and more scarce every day as rivers, lakes wells and even seawater pollution rapidly increases. Solar energy is one promising solution to secure power and potable water to future generation. The process of distillation can be used to obtain fresh water from salty, brackish or contaminated water. Water is available in different forms such as sea water, underground water, surface water and atmospheric water. Clean water is essential for good health. The search for sustainable energy resources has emerged as one of the most significant and universal concerns in the 21st century. Solar energy conversion offers a cost effective alternative to our traditional usages. Solar energy is a promising candidate in many applications. Among the alternative energy sources used for electricity production, wind and solar energy systems have become more attractive in recent years. For areas where electricity was not available, stand alone wind and solar systems have been increasingly used. The shortage of drinking water in many countries throughout the world is a serious problem. Humankind has depended for ages on river, sea water and underground water reservoirs for its fresh water needs. But these sources do not always prove to be useful due to the presence of excessive salinity in the water. To resolve this crisis, different methods of solar desalination have been used in many countries. Distillation is a well known thermal process for water purification, most importantly, water desalination. Most of the conventional water distillation processes are highly energy consuming and require fossil fuels as well as electric power for their operation. Single basin solar still is a popular solar device used for converting available brackish or waste water into potable water. Because of its lower productivity, it is not popularly used. Numbers of works are under taken to improve the productivity and efficiency of the solar still. There are large numbers of PCMs that melt and solidify at wide range of temperatures, making them attractive in a number of applications. PCMs have been widely used in latent heat thermal storage systems for heat pumps, solar engineering and spacecraft thermal control applications. The use of PCMs for heating and cooling applications for buildings has been investigated within the past decade. The experimental results computed in the field of water distillation process using solar energy in the presence of energy storage materials sodium sulphate and sodium acetate are discussed in this paper. Keywords: solar energy, saline water, distillation, phase change material.

The Effects of Salinity on Herring After Metamorphosis

1961 ◽  
Vol 41 (1) ◽  
pp. 37-48 ◽  
Author(s):  
F. G. T. Holliday ◽  
J. H. S. Blaxter
Keyword(s):  
Salinity Tolerance ◽  
Blood Concentration ◽  
Sea Water ◽  
Freezing Point ◽  
Tolerance Range ◽  
Blood Concentrations

The salinity tolerance of herring 9-ca 24 cm in length was found to lie between 6‰0 and 40–45‰0.Determinations of changes in weight and blood concentration (by measurement of the freezing-point), when herring were transferred from one salinity to another, demonstrated that extensive changes occurred in the blood. Under these conditions the herring experienced and survived blood concentrations equivalent to salinites of 13–22·5‰. A recovery to near normal (δ0·95 ≡ 15·8‰) took place in all the salinities within the tolerance range.Badly descaled herring in sea water showed large increases in blood concentration before death.A study of the kidney of the herring indicated that the ability to withstand the low salinities for long periods probably rested in the high glomerular count of the kidney.The importance of damage to the skin for survival is discussed in relation to tagging experiments.The results are also discussed in relation to the evolution of the herring.

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