GENETIC ANALYSIS OF NURSE DAMS SELECTED FOR SIX-WEEK BODY WEIGHT OR POSTWEANING GAIN IN MICE

1976 ◽  
Vol 18 (4) ◽  
pp. 611-623 ◽  
Author(s):  
J. Nagai ◽  
H. Bakker ◽  
E. J. Eisen
Keyword(s):  
Body Weight ◽  
Feed Intake ◽  
Selection Response ◽  
Genetic Effects ◽  
Control Group ◽  
Correlated Response ◽  
Population Differences ◽  
Correlated Responses ◽  
Maternal Performance ◽  
And Control

A modified crossfostering technique was developed to compare the performance of nurse dams in selected and control populations of mice. The H6 and M16 populations were selected for increased 6-week body weight and 3- to 6-week postweaning gain, respectively, while the C2 and ICR populations were the respective controls. Crossfostering was performed using H6, M16 and their reciprocal F1 crosses as nurse dams in the selected crossfostering group and C2, ICR and their reciprocals in the control group. Measurements recorded for nurse dams included mean body weight of 8 young within a nursed litter at birth (MWB) and 12 days of age (MW12). The latter was used as a measure of postnatal maternal performance. Other traits recorded for nurse dams were number born (NB), body weight at parturition (DWP) and 12 days postpartum (DW12), and weight gain (DWG), feed intake (FED) and efficiency (EFF = DWG/FED) for the first 12 days of lactation. The correlated response in MW12 was negative (P <.01) for M16 and essentially zero for H6. Both lines exhibited positive (P <.01) correlated responses in DWP and DW12 and no change in EFF. Only the H6 line increased significantly in DWG and FED as a result of selection. NB increased in M16 and H6, but was significant for the latter population only. Population differences in selection response [(M16-ICR)-(H6-C2)] were significant for FED only, primarily due to average direct genetic effects. Direct comparisons of M16 and H6 indicated that M16 was larger in DWP and DW12 but smaller in DWG and EFF. Average direct genetic effects favored M16 for NB, DWP, and DW12, whereas average maternal genetic effects favored H6 for NB, DWP, DW12 and FED. Percent direct heterosis in F1 crosses of the selected populations was significant for MW12 (13.7%), FED (10.8%) and NB (11.4%). Direct heterosis in F1 crosses of the controls was significant for MW12 (9.4%), NB(6.6%), DWP (3.5%), DW12 (3.3%) and FED (4.4%). The effects of MW12, DWG and metabolic body size (MBS) accounted for 47% of the variation in FED, pooled within populations. Of these variables, MW12 accounted for the highest proportion (32%) of variation in total feed intake.

scholarly journals PARTITIONING AVERAGE AND HETEROTIC COMPONENTS OF DIRECT AND MATERNAL GENETIC EFFECTS ON GROWTH IN MICE USING CROSSFOSTERING TECHNIQUES

Genetics ◽  
1976 ◽  
Vol 84 (1) ◽  
pp. 113-124
Author(s):  
J Nagai ◽  
H Bakker ◽  
E J Eisen
Keyword(s):  
Body Weight ◽  
Selection Response ◽  
Genetic Effects ◽  
Control Group ◽  
Correlated Responses ◽  
Secondary Importance ◽  
Body Weights ◽  
Weight Gains ◽  
The Individual ◽  
Linear Contrasts

ABSTRACT Crossfostering was performed using lines selected for increased 6-week body weight (H6) and increased 3-to 6-week postweaning gain (M16) and their reciprocal F1 crosses as nurse dams in the selected crossfostering group, and base population controls (C2, ICR) and their reciprocal F1 crosses in the control group. The offspring suckled were H6, M16 and F2 crosses in the selected group, and C2, ICR and their F2 crosses in the control group. Measurements taken on the individual offspring were body weights at birth (WB) and at 12, 21, 31, 42, and 63 days (W12, W21, W31, W42 and W63, respectively) and weight gains between adjacent ages (GB-12, G12–21, G21–31, G31–42 and G42–63, respectively). Least squares constants fitted to populations of genetic and nurse dams were used to calculate specific linear contrasts. Correlated responses to selection in average direct genetic effects were significant and positive for all traits examined in both H6 and M16, while the correlated responses in average maternal genetic effects were negative in M16 and negligible in H6. Selection response was primarily due to average direct genetic effects while the contribution of average maternal genetic effects was of secondary importance. The response in average direct genetic effects was smaller in M16 than in H6 through weaning (WB, W12 and W21), but was larger in M16 for postweaning weights (W31, W42 and W63). The correlated responses in average maternal genetic effects were consistently smaller in M16 than in H6. Direct heterosis was significant for all traits except for G12–21 and G42–63 in the control group, whereas maternal heterosis was significant for weight gains at early ages and for body weights. Direct heterosis tended to be larger than maternal heterosis in both selected and control crosses. Percent direct heterosis for body weight was larger in the selected crosses relative to the control crosses through 31 days of age, but the trend was reversed by 63 days. Percent maternal heterosis was consistently larger in the selected crosses.

DIRECT AND MATERNAL GENETIC RESPONSES RESULTING FROM SELECTION FOR 12-DAY LITTER WEIGHT IN MICE

1973 ◽  
Vol 15 (3) ◽  
pp. 483-490 ◽  
Author(s):  
E. J. Elsen
Keyword(s):  
Body Weight ◽  
Genetic Variance ◽  
Selection Response ◽  
Genetic Effects ◽  
Direct Effects ◽  
Litter Weight ◽  
Environmental Component ◽  
Selection For ◽  
Genetic Responses ◽  
And Control

Response to selection for 12-day litter weight in mice was evaluated in terms of average direct and average maternal genetic responses. Comparisons were made among selected and control parental lines, reciprocal F1 crosses, F2's and backcrosses. Selection response in 12-day body weight was primarily due to average direct genetic effects. Correlated responses in body weight at 21, 31, 42 and 70 days also were due to average direct effects. Average maternal genetic effects contributed little to direct or correlated weight responses. Heterosis for direct genetic effects was not significant for 12- and 21-day body weights but was important for postweaning weights. In contrast, heterosis for maternal genetic effects was important at 12 and 21 days but declined after weaning. These results suggest that the maternal genetic influence on neonatal mammalian growth may play a key role in fitness of the offspring. Thus, natural selection would tend to reduce additive maternal genetic variance, leaving mainly non-additive maternal genetic variance. This, coupled with the large maternal environmental component influencing neonatal growth, suggests that selection for additive direct effects influencing preweaning growth in livestock would be most efficiently carried out by standardizing or eliminating the postnatal maternal environment.

Impact of an Infant Formula Containing a Novel Fat Blend (Cow’s Milk Fat, Fish and Vegetable Oil) and Prebiotics on Stool Fatty Acid Soaps and Erythrocyte Fatty Acid Profiles in Full-Term Healthy Newborns

2021 ◽  
pp. 1-8
Author(s):  
Maroula Lambidou ◽  
Birgit Alteheld ◽  
Rolf Fimmers ◽  
Frank Jochum ◽  
Antonia Nomayo ◽  
...  
Keyword(s):  
Body Weight ◽  
Fatty Acid ◽  
Infant Formula ◽  
Milk Fat ◽  
Control Group ◽  
Human Breast Milk ◽  
Infant Formulas ◽  
Fa Composition ◽  
And Control ◽  
Breast Fed

<b><i>Introduction:</i></b> Recently, new commercial infant formulas have been composed considering novel fat blends and oligosaccharides to better resemble the fatty acid (FA) composition and stereospecific distribution (e.g., increased amount of ß-palmitate) as well as probiotics content of human breast milk. We hypothesized that these newly composed infant formulas may decrease fecal FA soap excretion and may positively affect erythrocyte FA profiles compared with regular formulas. <b><i>Methods:</i></b> Healthy infants were randomly assigned to receive a high-sn-2-palmitate formula (&#x3e;25% of the PA is esterified to the sn-2 position of the glycerol backbone, verum: <i>n</i> = 30) or a “standard” formula containing &#x3c;10% of PA in sn-2 position and no oligosaccharides (control: <i>n</i> = 27); a non-randomized group of breast-fed infants served as control. Anthropometric data of the infants (body weight, recumbent length, and head circumference) were recorded at inclusion (visit 1) and 6 and 12 weeks after onset of intervention (visits 2 and 3). Blood samples for erythrocyte FA analysis (gas chromatography) were taken at visits 1 and 2; stool samples were collected at visit 2. <b><i>Results:</i></b> Quantitative formula intake (mL/kg body weight × day) at visit 2 (verum: 155 ± 30, control: 164 ± 30) and visit 3 (verum: 134 ± 26, control: 134 ± 21) was comparable. Six weeks after onset of intervention, stool total FA soaps, palmitate soaps, and total FAs were similar in both formula-fed groups but significantly higher than in breast-fed infants. During the 6-week intervention, erythrocyte palmitate decreased significantly from baseline in all 3 groups with no group differences (verum: 29.20 ± 1.17 to 27.12 ± 0.66, control: 29.88 ± 2.00 to 27.01 ± 0.94, breast-fed: 30.20 ± 0.86 to 26.84 ± 0.98). For selected FAs, significant changes over time in verum and control group were obvious but without formula effects. Some variations in the FA profile of breast-fed infants compared to both verum and control groups were observed. <b><i>Conclusions:</i></b> In contrast to our hypothesis, feeding a newly composed infant formula based on a fat blend with 25% of PA in the sn-2 position of triacylglycerols and supplemented with a prebiotic could not decrease insoluble FA soap excretion compared with a standard product; in this respect, breastfeeding is obviously the best choice. Surprisingly, erythrocyte FA profiles were comparable in formula-fed and breast-fed infants; obvious alterations in FA composition of the respective fat sources and structure did not affect FA incorporation into membranes. Caution should be, however, exercised in drawing robust conclusions in the absence of larger, adequately powered intervention studies.

Significance of graded doses of aqueous seed extract of Moringa oleifera (L) on live body weight, gonadal, extragonadal dimensions and sperm reserves of Yankasa rams

2021 ◽  
pp. 33-40
Keyword(s):  
Body Weight ◽  
Moringa Oleifera ◽  
Seed Extract ◽  
The Body ◽  
Control Group ◽  
Live Body Weight ◽  
And Control ◽  
Standard Techniques ◽  
Different Doses ◽  
Apparently Healthy

The aim of the study was to investigate the effects of Moringa oleifera aqueous seed extract on live body weight, gonadal and extragonadal dimensions and sperm reserves of Yankasa rams. Twenty five apparently healthy Yankasa rams aged 1-2 years and weighing 19.0 ± 2.1 Kg were used for the study. The rams were randomly selected into five groups: A, B, C, D and E with five rams in each group as treatment and control groups respectively. Groups A - D were given oral dose of Moringa oleifera aqueous seed extract at a dose rate of 1000, 2000, 3000, 4000 (mg/kg), respectively while group E was given 10 ml/kg water orally, daily for five months. Live body weight, gonadal and extragonadal reserves were determined according to standard techniques. The results showed a significant increase in live body weight in the months of April to June among rams treated with different doses of Moringa oleifera aqueous seed extract compared with the control group. The control group showed no significant differences in the body weight, gonadal and extragonadal dimensions and sperm reserves. In conclusion, the treatment of Yankasa rams with Moringa oleifera aqueous seed extract increased live body weight, but had no significant effects on gonadal and extragonadal dimensions and sperm reserves in Yankasa rams. Therefore, it is recommended that M. oleifera aqueous seed extract can be used at doses of 2000mg/kg to 3000mg/kg in Yankasa rams for optimum gain in live body weight.

DIRECT GENETIC AND POSTNATAL MATERNAL GENETIC EFFECTS ON BODY COMPOSITION IN MICE SELECTED FOR BODY WEIGHT

1979 ◽  
Vol 21 (1) ◽  
pp. 43-56 ◽  
Author(s):  
J. F. Hayes ◽  
E. J. Eisen
Keyword(s):  
Body Weight ◽  
Genetic Effects ◽  
The Body ◽  
Control Line ◽  
Ether Extract ◽  
Correlated Responses ◽  
Relative Growth Rates ◽  
Order Of Magnitude ◽  
Body Components ◽  
Water Percent

Line crossfostering techniques were used to study differences among selected and control lines of mice in direct genetic and postnatal maternal genetic influences on preweaning (day 12) body weight and composition. The lines were selected for high (H6) and low (L6) 6-week body weight and the control line (C2) was maintained by random selection. There were positive correlated responses to selection in both direct genetic and postnatal maternal genetic effects on body weight and weights of all body components (P < 0.01) except for water and ash weight in H6. The correlated responses in postnatal maternal genetic effects were of the same order of magnitude as those in direct genetic effects. Correlated responses were greater in L6 than in H6. Correlated responses in direct genetic effects were positive (P < 0.01) for water percent in H6 and ether extract percent in L6, and negative (P < 0.01) for water percent and lean percent in L6. Correlated responses in postnatal maternal genetic effects were positive for ether extract percent and negative for water percent (P < 0.01). Correlated responses were far greater in L6 than in H6 and were greater for postnatal maternal genetic effects than for direct genetic effects. Analyses of covariance results indicated line differences in the relative growth rates of the body components.

scholarly journals Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

2020 ◽  
Vol 20 (07) ◽  
pp. 16984-16996
Author(s):  
MMC Anyakudo ◽  
◽  
DO Adeniji ◽  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Morphological Features ◽  
High Protein ◽  
Control Group ◽  
Formulated Diet ◽  
Low Carbohydrate ◽  
Male Wistar Rats ◽  
And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

scholarly journals Tempe Nuggets Provision Improves Energy Adequacy and Protein Intake in Underweight Underfive Children

2018 ◽  
Vol 6 (1) ◽  
pp. 89-96 ◽  
Author(s):  
Oktavina Permatasari ◽  
Retno Murwani ◽  
M. Zen Rahfiludin
Keyword(s):  
Body Weight ◽  
Energy Intake ◽  
Protein Intake ◽  
Local Community ◽  
Intervention Group ◽  
Control Group ◽  
Post Intervention ◽  
Control Group Design ◽  
Soybean Food ◽  
And Control

Tempe is a well known fermented soybean food, inexpensive, and a good source of dietary protein and energy. To improve fresh Tempe as an attractive food to children, Tempe is processed into nugget. A study using pre and post control group design was conducted to determine the effect of the Tempe nugget provision on energy and protein intake, and body weight/age (W/A) of 24-59 months old children. Forty six subjects were selected according to inclusion criteria from local Community Health Center (Puskesmas) and grouped into intervention (provision of Tempe nugget) and control group (no provision). Data of energy and protein intake of subjects before intervention were obtained by 2x24 food recall to determine deficiency in intake for the respective age. The amount of the nugget given to each underweight child in intervention group was calculated on the basis of deficiency in energy and protein intake of each child (W/A) per day. Body weight was recorded pre and post intervention. The deep-fried nuggets contained 276.53 calories per 100 g, 8.60% protein, 28.41% carbohydrate, 13.28% lipid, and 44.28% fiber. The mean age of the subjects in intervention and control group were homogeneous i.e. 40.52±10.88 months and 42.39±12.35 months respectively. Tempe nugget provision for 30 days improved significantly energy intake (p 0.001) in intervention compared to control group. Protein intake and W/A in intervention group were higher compared to control although not significant and further study with higher amount of Tempe nugget provision is needed. This study provides evidence that deep fried Tempe nugget can be used as inexpensive and nutritious food to improve protein and energy intake for underweight underfive children.

scholarly journals LONG-TERM SELECTION RESPONSE FOR 12-DAY LITTER WEIGHT IN MICE

Genetics ◽  
1972 ◽  
Vol 72 (1) ◽  
pp. 129-142
Author(s):  
E J Eisen
Keyword(s):  
Body Weight ◽  
Genetic Correlation ◽  
Half Life ◽  
Selection Process ◽  
Selection Response ◽  
Exponential Model ◽  
Correlated Response ◽  
Term Selection ◽  
Litter Weight

ABSTRACT Long-term selection for increased 12-day litter weight in two replicate lines (W2, W3) of mice resulted in an apparent selection limit at about 17 generations. Quadratic polynomial and exponential models were fitted to the data in order to estimate the plateaued response and half-life of the selection process. Using the polynomial results, the half-life estimates were 4.5 and 8.6 generations for W2 and W3, respectively. The plateaued responses were 5.1 and 5.8 g which, when expressed in phenotypic standard deviation units, became 1.1 and 1.3. The exponential model provides similar estimates. A negative association between 12-day litter weight and fitness was not considered to be an adequate explanation for the plateau since there was no decrease in fertility of the selected lines. Evidence that exhaustion of genetic variability was not the cause of the plateau came from the immediate response to reverse selection. It was proposed that the plateau may be due to a negative genetic correlation between direct and maternal genetic effects, which would be expected to occur after many generations of selection. There were positive correlated responses in both replicates for adult body weight, which was in agreement with the positive genetic correlation between preweaning and postweaning body weight. The expected positive correlated response for number born was realized in only one of the replicates.

scholarly journals Toxicity Evaluation of a Traditional Polyherbal Unani Formulation Jawarish Shahi in Rats

2018 ◽  
Vol 7 (5) ◽  
pp. 412-418
Author(s):  
Mohd Urooj ◽  
◽  
Mohammad Ahmed Khan ◽  
G. Thejaswini ◽  
Munawwar Husain Kazmi ◽  
...  
Keyword(s):  
Body Weight ◽  
Feed Intake ◽  
Clinical Signs ◽  
Female Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Male And Female ◽  
Salix Caprea ◽  
Male And Female Rats ◽  
Dose Levels

Jawarish Shahi (JS) is a compound polyherbal Unani pharmacopoeial formulation indicated for Khafqan (Palpitation), Nafkh-e-Shikam (Flatulence) and Waswas (Insanity; false perception and hallucinations). Jawarish Shahi contains herbs like Halela (Terminalia chebula), Amla (Emblica officinalis), Kishneez (Coriandrum sativum), Elaichi Khurd, (Elettaria cardamomum), and Bed Mushk (Salix caprea). The present study was carried out as per OECD 408 guidance to evaluate 90 days repeated oral dose toxicity in male and female Sprague Dawley rats. The study was performed at dose levels 1028 and 2000 mg/kg bw. No adverse effects were reported with respect to body weight, feed intake, behavior and clinical signs indicative of systemic toxicity. The expected growth pattern was observed in body weight and feed intake as compared to control group at both dose levels in male and female rats. There were few significant alterations with respect to hematology, and clinical biochemistry, however the results were within normal range thus considered toxicologically insignificant. The microscopic examination of different organ/tissue showed that no histopathological changes were observed. The findings of the study showed that No Observed Adverse Effect Level (NOAEL) for JS is greater than 2000 mg/kg body weight

Effect of exercise duration on feed intake and body composition of Swiss albino mice

1985 ◽  
Vol 58 (2) ◽  
pp. 500-505 ◽  
Author(s):  
R. Bulbulian ◽  
K. K. Grunewald ◽  
R. R. Haack
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Protein Content ◽  
Feed Intake ◽  
Exercise Duration ◽  
The Body ◽  
Control Group ◽  
Swiss Albino Mice ◽  
Daily Exercise ◽  
Albino Mice

The purpose of this study was to examine the effects of daily exercise of varying duration on the body composition, weight, and feed intake of mature Swiss albino mice. Fifty-four male mice were equally divided into a control group and five exercise groups (n = 9) performing 20, 40, 60, 120, and 240 min of daily exercise on a treadmill (7.2 m/min). Feed intake and body weight were measured weekly for 10 wk. At the completion of the study the mice were killed and the animal carcasses were chemically analyzed for fat, dry matter, and protein content. The results of this study demonstrate no differences in the body weight among groups (P less than 0.97) with all groups gaining 4.5–5.8 g during the 10-wk period. However, fat content decreased significantly from 15.7% in the control to 12.0% in the 120- and 240-min exercise groups (P less than 0.05). In contrast, protein content showed an insignificant rising trend from 13.0 to 14.6% with increasing duration of exercise. Feed intake showed a nonsignificant drop during the 20-min exercise treatment and remained unchanged among groups. These data show a slight but variable appetite-suppressing effect of light exercise in mice accompanied by favorable body composition changes even in the absence of differences in body weight. These findings suggest the mouse to be an acceptable experimental model for body composition and exercise studies.

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