A single molecular marker to distinguish between species of Dioscorea

Genome ◽  
2017 ◽  
Vol 60 (3) ◽  
pp. 201-207 ◽  
Author(s):  
Natascha Techen ◽  
Iffat Parveen ◽  
Ikhlas A. Khan
Keyword(s):  
Molecular Marker ◽  
Genomic Region ◽  
Dioscorea Alata ◽  
Aerial Parts ◽  
Discrimination Rate ◽  
The World ◽  
Single Locus Analysis ◽  
Identification Of Species ◽  
Dioscorea Bulbifera ◽  
Improve Health

Yams are species of the genus Dioscorea (family Dioscoreaceae), which consists of approximately 630 species. The majority of the world production of yams occurs in Africa with 58.8 million t annually, but they are also produced in the Americas and Asia. The saponins in yams have been reported to possess various properties to improve health. The tuber and aerial parts of various species often share morphological similarities, which can cause problems in the proper identification of sample material. For example, the rootstocks and aerial parts of Dioscorea villosa L. share similarities with Dioscorea polystachia Turcz. Dioscorea bulbifera L. may be mistaken for Dioscorea alata L. owing to similar morphologies. Various molecular analyses have been published to help with the identification of species and varieties within the genus Dioscorea. The multi-loci or single-locus analysis has resulted in varying success, some with only a limited discrimination rate. In the present study, a single nuclear genomic region, biparentally inherited, was analyzed for its usefulness as a molecular marker for species identification and discrimination between D. bulbifera, D. villosa, D. nipponica, D. alata, D. caucasica, and D. deltoidea samples. The results of this study show that the LFY genomic region can be useful as a molecular marker to distinguish between samples.

HUBUNGAN ANTARA PENDIDIKAN DAN STATUS EKONOMI IBU DENGAN KEJADIAN DIARE PADA BALITA DI PUSKESMAS SEKIP PALEMBANG TAHUN 2014

2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Wulan Citra Sari Wulan Citra Sari
Keyword(s):  
Economic Status ◽  
Chi Square ◽  
Cross Sectional ◽  
Diarrhea Case ◽  
The World ◽  
Diarrhea Disease ◽  
United Nation ◽  
Research Show ◽  
Improve Health ◽  
Children Under 5

  ABSTRACT Diarrhea is cause the number one of a death in the world. The united Nation Children’s Found (UNICEF) thinks about every 30 second there one a child die because diarrhea. Diarrhea disease still be a problem for society healthy in Indonesia well reviewed of morbidity and mortality ant the mark of deaths and “Kejadian Luar Biasa (KLB)” that caused. Accrodin to WHO the mark of diarrhea illness 2010 is 411 patients per 1.000 population. The digit of death diarrhea is 14% from the digit of death over all. The purpose of this research to know a connection between education and mother economic status with toodler (children under 5 years old) diarrhea case at Puskesmas Sekip Palembang in February 2014. Desaign of this research to survey analitic by do coloser Cross Sectional in Accidental Sampling tehnic which one the variable who get research, include education and mothers economic status. The sampel in this research in 33 mothers who come by bring their toodler to MTBS Puskesmas Sekip Palembang at February 2014. Each variabel who get research, then get analysis by use analysis Chi-Square by decisios X² table (3,481). The result of this research show that the respondent by diarrhea as many is 15 respondent (45,5%) and didn’t diarrhea 18 respondent (54,5%). This of high education 13 repondent (39,4%) and less education is many as 20 respondent (60,6%), the respondent based to the high economic status as many as 13 respondent (39,4%). From statistic test Chi-Square show that, there a connection between education  with diarrhea case in X² count = 4,313, and there a connection between mothers economic status with case diarrhea at X² count= 4,917. From the results of this study are expected to further improve health education, especially regarding the incidence of diarrhea in infants to mothers who have young children understand more about the incidence of diarrhea.     ABSTRAK Diare adalah penyebab nomor satu kematian di dunia. The United Nations Children’s Fund (UNICEF) memperkirakan bahwa setiap 30 detik ada anak meninggal karena diare. Penyakit diare masih merupakan masalah kesehatan masyarakat di Indonesia baik ditinjau dari angka kesakitan dan angka kematian serta kejadian luar biasa (KLB) yang ditimbulkan. MenurutWHO angka kesakitan diare pada tahun 2010 yaitu 411 penderita per 1000 penduduk. Angka kematian diare 14% dari angka kematian balita secara keseluruhan. Tujuan penelitian ini untuk mengetahui hubungan antara pendidikan dan status ekonomi ibu dengan kejadian diare pada balita di Puskesmas SekipPalembang pada bulan Februari Tahun 2014. Desain penelitian ini adalah survey analitik dengan pendekatan Cross Sectional dalam teknik Accidental Sampling dimana variableyang diteliti meliputi pendidikan dan status ekonomi ibu. Sampel pada penelitian ini adalah 33ibu yang datang membawa anak balitanya berobat ke MTBS puskesmas Sekip Palembang pada bulan FebruariTahun 2014. Masing-masing variabel yang diteliti kemudian dianalisa dengan menggunakan analisis Chi-Square dengan ketetapan X² Tabel (3,481). Hasil penelitian ini menunjukan bahwa responden dengan diare sebanyak 15 responden (45,5%) dan tidak diare 18 responden (54,5%), responden berdasarkan pendidikan tinggisebanyak 13responden (39,4%) dan pendidikan rendah sebanyak 20 responden (60,6%), responden berdasarkan status ekonomi tinggi sebanyak 20 responden (60,6%) dan yang status ekonomi rendah sebanyak 13 responden (39,4%). Dari uji statistik Chi-Square menunjukkan ada hubungan antara pendidikan dengan kejadian diare pada X²hitung= 4,313, dan ada hubungan antara status ekonomi ibu dengan kejadian diare pada X²hitung= 4,917. Dari hasil penelitian ini diharapkan agar lebih meningkatkan penyuluhan kesehatan khususnya tentang kejadian diare pada balita agar ibu yang mempunyai balita lebih memahami tentang kejadian diare.  

scholarly journals Effects of Long-Wave UV Light on Monilinia Growth and Identification of Species

Plant Disease ◽  
1999 ◽  
Vol 83 (1) ◽  
pp. 62-65 ◽  
Author(s):  
A. De Cal ◽  
P. Melgarejo
Keyword(s):  
Short Distance ◽  
Mycelial Growth ◽  
Uv Light ◽  
Dark Period ◽  
Morphological Characteristics ◽  
Potato Dextrose Agar ◽  
Long Wave ◽  
The World ◽  
Identification Of Species ◽  
Respective Species

The effect of long-wave UV/dark period on mycelial growth of 46 isolates of Monilinia sp. collected in Spain and 16 isolates collected from other parts of the world was investigated. Typical isolates of M. laxa, M. fructigena, and M. fructicola were grown in the dark and identified by morphological characteristics. Long-wave UV/dark conditions reduced the growth rates of M. laxa, M. fructigena, and M. fructicola on potato dextrose agar. All isolates of M. fructigena grew more slowly than those of M. fructicola. Typical and atypical isolates of M. fructigena and M. fructicola were placed in their respective species based on long-wave UV/dark growth rate data. M. laxa isolates were readily distinguished by the short distance from their conidium to the first germ tube branch. The involvement of different photoreceptors in photoresponses by M. fructicola and M. fructigena is discussed. Differences in mycelial growth under long-wave UV may be a useful tool to identify Monilinia spp.

scholarly journals 2014 Geneva Declaration on Person- and People-centered Integrated Health Care for All

2015 ◽  
Vol 4 (2) ◽  
pp. 66-68
Author(s):  
Juan Mezzich
Keyword(s):  
Health Care ◽  
Cost Effective ◽  
World Health ◽  
Universal Health Care ◽  
The World ◽  
International College ◽  
Commission Report ◽  
Geneva Conference ◽  
Universal Health ◽  
Improve Health

We, participants in the 7th Geneva Conference on Person-centered Medicine, call on everyone to join together to promote person-centered and people-centered health care in order to improve health for all in ways that are equitable, sustainable, and cost-effective. Equity and integration in person-centered health care are crucial foundations for targeting opportunities for effective action. The International College of Person-Centered Medicine (ICPCM) adheres to the new perspectives on universal health care endorsed by the World Health Assembly since 2009 and reads with interest the 2013 Lancet Commission Report on “Global Health 2035:  World converging within a generation”, both of which outline objectives, research, and strategies for developing Person- and People-centered Integrated Care (PPCIC) for all people

scholarly journals MIRACLE AYURVEDIC HERB - ASHWAGANDHA (WITHANIA SOMNIFERA DUNAL)

2021 ◽  
Vol 9 (8) ◽  
pp. 1778-1788
Author(s):  
Bargale Sushant Sukumar
Keyword(s):  
Withania Somnifera ◽  
Chemical Constituents ◽  
Immunomodulatory Activity ◽  
Indian Ginseng ◽  
Aerial Parts ◽  
Wide Range ◽  
The World ◽  
Anti Inflammatory ◽  
Ayurvedic Herb ◽  
Present World

Ayurveda is successfully implemented as a primary healthcare system in India, and it is flourishing in the promotion of health around the world. Ayurveda aims to create a society happily, healthy, and peaceful. In general, the simple regimes mentioned in Ayurveda texts are of immense use faced by the present world today regarding health pro- motion. Ashwagandha (Withania Somnifera) is usually referred to as 'Indian winter cherry' or 'Indian Ginseng'. It is a shrub grown in India and North America whose roots have been used by Ayurvedic practitioners for thousands of years. Ashwagandha is one of Ayurveda's most essential herbs, used as Rasayana for its wide range of health benefits for centuries. Chemicals constitute of Ashwagandha (Withania Somnifera)- The chemistry of Ashwagan- dha (Withania Somnifera) has been widely studied and numerous categories of chemical constituents have been described, extracted, and isolated, such as steroidal lactones, alkaloids, flavonoids, tannin, etc. More than 12 alka- loids, 40 withanolides and several sitoindosides have currently been isolated from the aerial parts, roots, and berries of the Withania species and have been recorded. The principal biochemical element of Ashwagandha (WS) root is withanolides, which are steroidal alkaloids and steroidal lactones. The pharmacological activity of Ashwagan- dha- It has been shown to have pharmacological importance as an Adaptogen, Antibiotic, abortifacient, aphrodisiac, Astringent, Anti-inflammatory, deobstructive, diuretic, narcotic, sedative, and tonic through centuries of Ayurvedic medicinal practice using Ashwagandha (Withania Somnifera). Along with these Ashwagandha acts as Anti-stress,Anticarcinogenic activity, Anti-inflammatory activity, Anti-aging activity, Cardioprotective activity, hypothyroid activity and Immunomodulatory activity. Keywords: Ashwagandha, Withania Somnifera, Alkaloids, Withanoids, sitoindosides

Visioning the future of health promotion

2021 ◽  
pp. 175797592110357
Author(s):  
Ilona Kickbusch
Keyword(s):  
Health Promotion ◽  
Human Impact ◽  
The Public ◽  
Health And Wellbeing ◽  
Public Purpose ◽  
The World ◽  
The Future ◽  
Sustainable Societies ◽  
Transformative Approach ◽  
Improve Health

COVID-19 has shown us clearly that the world must commit to a transformative approach that promotes health and wellbeing. Living in the Anthropocene – an epoch defined by human impact on our ecosystems – moves us into unknown territory. The challenge is to find a way of living that aims to meet the needs of all people within the means of the living planet. We will require foresight, agility and resilience to be well prepared. The global risks we face are enormous and they are interconnected – yet the opportunity to accelerate change for the better is extraordinary as well. We have models, knowledge and technologies at our disposal that could significantly improve health and wellbeing and create fairer and more sustainable societies – yet they have not been used widely to serve the public purpose and to address inequities.

scholarly journals Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Marina Carrara Dias ◽  
Bruna Stuqui ◽  
Paola Jocelan Scarin Provazzi ◽  
Cíntia Bittar ◽  
Natália Maria Candido ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Amino Acid ◽  
Hpv Infection ◽  
Clinical Findings ◽  
Genomic Region ◽  
Geographical Region ◽  
Specific Primers ◽  
The World ◽  
Hpv Types ◽  
Viral Sequences

Condyloma acuminata (CA), or genital warts, are benign proliferative epidermal or mucous lesions that are caused by infection with human papillomavirus (HPV), mainly the low-risk types 6 and 11. HPV variants are defined as viral sequences that share identity in the nucleotide sequence of the L1 gene greater than 98%. Based on this criterion, HPV6 and 11 variant lineages have been studied, and there are ongoing attempts to correlate these genetic variants with different clinical findings of infection. Therefore, the aims of this study were to detect variants and nucleotide alterations present in the E6 regions of HPV types 6 and 11 found in CA samples, to correlate the HPV presence with the clinical-pathological data of the patients, and to determine phylogenetic relationships with variants from other places in the world. The E6 regions of 25 HPV6 samples and 7 HPV11 samples from CA were amplified using PCR with specific primers. The products were ligated to a cloning vector and five colonies of each sample were sequenced to observe the nucleotide alterations. Twelve samples were identified as the HPV6B3 variant, presenting the mutation (guanine) G474A (adenine), and one of them also showed the mutation (thymine) T369G. The other 13 patients were positive for HPV6B1 without nucleotide alterations. In the analysis of the HPV11 samples, all patients showed the mutations T137C and (cytosine) C380T. One patient also presented the nucleotide alteration T410C. None of the mutations found in the 32 analyzed samples resulted in amino acid changes. Patient age, local occurrence, and HIV infection did not show significant association with HPV infection. Besides, the data found in this study did not show a relationship with the geographical region of isolation when compared to other data from different regions of the world. In this way, despite the nucleotide alterations found, it was not possible to observe amino acid changes and variants grouping according to geographical region.

Taxonomic notes and new synonyms on Brazilian Macromitrium Bridel (Bryophyta, Orthotrichaceae)

Phytotaxa ◽  
2020 ◽  
Vol 454 (3) ◽  
pp. 213-225
Author(s):  
DAIANE VALENTE VALENTE ◽  
DENILSON FERNANDES PERALTA ◽  
RENATO XAVIER ARAÚJO PRUDÊNCIO ◽  
PAULO EDUARDO AGUIAR SARAIVA CÂMARA
Keyword(s):  
Optical Microscopy ◽  
Correct Identification ◽  
Type Specimens ◽  
Important Data ◽  
Flora Of Brazil ◽  
The Third ◽  
New Synonyms ◽  
The World ◽  
Identification Of Species ◽  
Brazilian Flora

Brazil is a megadiverse country that intends to catalog all its flora by 2020. Therefore, knowledge about taxonomy and the correct identification of species is essential for accessing the real species biodiversity. Macromitrium (Orthotrichaceae) is considered the third largest moss genus in the world and with the majority of its species distributed in tropical and subtropical regions. For Brazil 64 species have been cited, but 44 remain as unknown to the Brazilian flora. The aim of this work was to check all 64 names of Brazilian Macromitrium helping to clarify its validity and correct identification. Type specimens from 14 herbaria were studied using optical microscopy. After this analysis 22% are good species, 54% are synonymous of others species; 13% are excluded from the Brazilian flora and 11% were not possible to check. This work contributed to clarify the diversity of Macromitrium in Brazil, contributing to the knowledge of bryophytes and important data for the flora of Brazil 2020.

scholarly journals Junipers of Various Origins as Potential Sources of the Anticancer Drug Precursor Podophyllotoxin

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5179
Author(s):  
Diana I. Ivanova ◽  
Paraskev T. Nedialkov ◽  
Alexander N. Tashev ◽  
Marta Olech ◽  
Renata Nowak ◽  
...  
Keyword(s):  
Comparative Investigation ◽  
Bioactive Metabolites ◽  
Leaf Extracts ◽  
Natural Sources ◽  
The World ◽  
Identification Of Species ◽  
Ancient Times ◽  
Folk Remedies ◽  
Sinopodophyllum Hexandrum ◽  
Antiproliferative Activities

Juniper representatives are natural sources of plenty of bioactive metabolites and have been used since ancient times as folk remedies against tapeworms, warts, cancer, etc. The antiproliferative activities of junipers are attributed to podophyllotoxin (PPT), which is a precursor for the synthesis of efficient anticancer drugs. However, the natural sources of PPT, Sinopodophyllum hexandrum (Royle) T. S. Ying and Podophyllum peltatum L., are already endangered species because of their intensive industrial exploitation. Therefore, identification of other sources of PPT is necessary. This study is a broad comparative investigation of junipers, for which original sources have been accessed from different continents of the world. The present research is aimed at the identification of species, producing PPT and other lignans at concentrations that are sufficient for the high antiproliferative activity of the corresponding extracts. Cytotoxic juniper leaf extracts demonstrated a broad spectrum of activity on a panel of cancer cell lines. The antiproliferative properties of junipers were attributed to the combined activity of great diversity of lignans (podophyllotoxin, deoxypodophyllotoxin, β-peltatin, yatein, matairesinol, anhydropodorhizol, etc.), detected by UHPLC-HRMS and LC-ESI-MS/MS in the corresponding extracts. Several species of the genus Juniperus L. were outlined as perspective sources of drug precursors with potential pharmaceutical applications.

Sign in / Sign up

Export Citation Format

Share Document