Anti-adhesion properties of  aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa

Introduction. The present study assessed Pseudomonas aeruginosa surface characteristics, motility and adhesion properties under the influence of 1-[4-(1,1,3,3-tetra methyl butyl) phenoxy]-3-(N-benzyl hexa methylene iminium)-2-propanol chloride (KVM-194). Material and methods. The clinical strain P. aeruginosa 449 was used in the study. The cell surface hydrophobicity (CSH) was evaluated by adhesion to solvent (MATS test). Swimming, swarming and twitching motility of P. aeruginosa were studied by standard methods in media with different agar contents. Cells ability to adhere to polystyrene was assessed by the Christensen method. The effect of KVM-194, meropenem and ciprofloxacin on hydrophobicity and motility was evaluated both at 0.5 or 2.0 minimal inhibitory concentrations (MIC), while on adhesion abilities – only 0.5×MIC. Results. It was shown that 0.5× MIC KVM-194 reduced CSH of P. aeruginosa (by 16%, p˂0.05), affected swimming motility, and decreased its adhesion to polystyrene. The most pronounced changes in adhesion properties were recorded after 3-5 hours of pre-treatment with this compound. Moreover, it was proven that sub-MICs of meropenem and ciprofloxacin did not alter bacterial cells hydrophobicity and had no significant influence on P. aeruginosa motility and adhesion properties. Conclusions. The present study suggested that KVM-194 affected the initial steps of P. aeruginosa biofilm formation and thus had tremendous potential for new antibiofilm agents’ development.

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Surface hydrophobicity of acidophilic heterotrophic bacterial cells in relation to their adhesion on minerals

Canadian Journal of Microbiology ◽

10.1139/m91-117 ◽

1991 ◽

Vol 37 (9) ◽

pp. 692-696 ◽

Cited By ~ 11

Author(s):

B. K. Chakrabarti ◽

P. C. Banerjee

Keyword(s):

Heterotrophic Bacteria ◽

Ph Value ◽

Manganese Nodule ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Bacterial Attachment ◽

Bacterial Cells ◽

Iron Pyrite ◽

Acidophilic Bacteria ◽

Hydrophilic Nature

The cell-surface hydrophobicity of acidophilic heterotrophic bacteria originating from mines varied with the pH of the suspending medium and with the growth temperature. Adhesion of these bacterial cells on mineral particles depended upon the hydrophobic (or hydrophilic) nature of both the cells and the minerals. A strong correlation between these properties was usually observed at different pH values of the suspending medium. At a certain pH value, bacterial attachment depended upon the particle size of the minerals. Key words: hydrophobicity, acidophilic bacteria, Acidiphilium cryptum, Acidiphilium symbioticum, adhesion, manganese nodule, chalcopyrite, iron pyrite.

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Bacterial factors influencing adhesion of Pseudomonas aeruginosa strains to a poly(ethylene oxide) brush

Microbiology ◽

10.1099/mic.0.29005-0 ◽

2006 ◽

Vol 152 (9) ◽

pp. 2673-2682 ◽

Cited By ~ 71

Author(s):

Astrid Roosjen ◽

Henk J. Busscher ◽

Willem Norde ◽

Henny C. van der Mei

Keyword(s):

Pseudomonas Aeruginosa ◽

Ethylene Oxide ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Contact Angles ◽

Bacterial Strains ◽

Water Contact ◽

Poly Ethylene Oxide ◽

Poly Ethylene ◽

Brush Coating

Most bacterial strains adhere poorly to poly(ethylene oxide) (PEO)-brush coatings, with the exception of a Pseudomonas aeruginosa strain. The aim of this study was to find factors determining whether P. aeruginosa strains do or do not adhere to a PEO-brush coating in a parallel plate flow chamber. On the basis of their adhesion, a distinction could be made between three adhesive and three non-adhesive strains of P. aeruginosa, while bacterial motilities and zeta potentials were comparable for all six strains. However, water contact angles indicated that the adhesive strains were much more hydrophobic than the non-adhesive strains. Furthermore, only adhesive strains released surfactive extracellular substances, which may be engaged in attractive interactions with the PEO chains. Atomic force microscopy showed that the adhesion energy, measured from the retract curves of a bacterial-coated cantilever from a brush coating, was significantly more negative for adhesive strains than for non-adhesive strains (P<0.001). Through surface thermodynamic and extended-DLVO (Derjaguin, Landau, Verwey, Overbeek) analyses, these stronger adhesion energies could be attributed to acid–base interactions. However, the energies of adhesion of all strains to a brush coating were small when compared with their energies of adhesion to a glass surface. Accordingly, even the adhesive P. aeruginosa strains could be easily removed from a PEO-brush coating by the passage of a liquid–air interface. In conclusion, cell surface hydrophobicity and surfactant release are the main factors involved in adhesion of P. aeruginosa strains to PEO-brush coatings.

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Cell surface characteristic of Asaia bogorensis – spoilage microorganism of bottled water

Czech Journal of Food Sciences ◽

10.17221/96/2011-cjfs ◽

2011 ◽

Vol 29 (No. 4) ◽

pp. 457-461 ◽

Cited By ~ 10

Author(s):

P. Sedláčková ◽

M. Čeřovský ◽

I. Horsáková ◽

M. Voldřich

Keyword(s):

Cell Surface ◽

Hydrophobic Interactions ◽

Cell Surface Hydrophobicity ◽

Surface Characteristics ◽

Surface Hydrophobicity ◽

Resistance Mechanisms ◽

Surface Characteristic ◽

Bottled Water ◽

Solid Surfaces ◽

Plastic Materials

The ability of bacteria to attach to a surface and develop a biofilm has been of considerable interest for many groups in the food industry. Biofilms may serve as a chronic source of microbial contamination and the research into biofilms and cells interactions might help to improve general understanding of the biofilm resistance mechanisms. Multitude of factors, including surface conditioning, surface charge and roughness and hydrophobicity, are thought to be involved in the initial attachment. Hydrophobic interactions have been widely suggested as responsible for much of the adherence of cells to surfaces. Cell-surface hydrophobicity is an important factor in the adherence and subsequent proliferation of microorganisms on solid surfaces and at interfaces. In the present study, we have estimated the cell-surface characteristics of Asaia bogorensis – isolated contamination of flavoured bottled water and compared its ability to colonise surfaces which are typical in the beverage production – stainless steel, glass and plastic materials.

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Adsorption of monorhamnolipid and dirhamnolipid on two Pseudomonas aeruginosa strains and the effect on cell surface hydrophobicity

Applied Microbiology and Biotechnology ◽

10.1007/s00253-008-1461-y ◽

2008 ◽

Vol 79 (4) ◽

pp. 671-677 ◽

Cited By ~ 63

Author(s):

Hua Zhong ◽

Guang Ming Zeng ◽

Jian Xiao Liu ◽

Xiang Min Xu ◽

Xing Zhong Yuan ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Cell Surface ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity

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Coral-Associated Bacteria as a Promising Antibiofilm Agent against Methicillin-Resistant and -SusceptibleStaphylococcus aureusBiofilms

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/862374 ◽

2012 ◽

Vol 2012 ◽

pp. 1-16 ◽

Cited By ~ 41

Author(s):

Shanmugaraj Gowrishankar ◽

Nyagwencha Duncun Mosioma ◽

Shunmugiah Karutha Pandian

Keyword(s):

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Multidrug Resistant ◽

Significant Inhibition ◽

Antibiofilm Activity ◽

Adhesion Properties ◽

Methicillin Resistant ◽

Cellular Components ◽

Promising Source

The current study deals with the evaluation of two coral-associated bacterial (CAB) extracts to inhibit the biofilm synthesisin vitroas well as the virulence production like hemolysin and exopolysaccharide (EPS), and also to assess their ability to modify the adhesion properties, that is cell surface hydrophobicity (CSH) of methicillin-resistant (MRSA) and -susceptibleStaphylococcus aureus(MSSA). Out of nine CAB screened, the ethyl acetate extract of CAB-E2 (Bacillus firmus) and CAB-E4 (Vibrio parahemolyticus) have shown excellent antibiofilm activity againstS. aureus. CAB-E2 reduced the production of EPS (57–79%) and hemolysin (43–70%), which ultimately resulted in the significant inhibition of biofilms (80–87%) formed by both MRSA and MSSA. Similarly, CAB-E4 was also found to decrease the production of EPS (43–57%), hemolysin (43–57%) and biofilms (80–85%) of test pathogens. CLSM analysis also proved the antibiofilm efficacy of CAB extracts. Furthermore, the CAB extracts strongly decreased the CSH ofS. aureus. Additionally, FT-IR analysis ofS. aureustreated with CAB extracts evidenced the reduction in cellular components compared to their respective controls. Thus, the present study reports for the first time,B. firmus—a coral-associated bacterium, as a promising source of antibiofilm agent against the recalcitrant biofilms formed by multidrug resistantS. aureus.

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Autoinducer Analogs Can Provide Bactericidal Activity to Macrolides in Pseudomonas aeruginosa through Antibiotic Tolerance Reduction

Antibiotics ◽

10.3390/antibiotics11010010 ◽

2021 ◽

Vol 11 (1) ◽

pp. 10

Author(s):

Mizuki Abe ◽

Keiji Murakami ◽

Yuka Hiroshima ◽

Takashi Amoh ◽

Mayu Sebe ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Membrane Permeability ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Cell Membrane Permeability ◽

New Combination ◽

Antibiotic Tolerance ◽

Bacterial Quorum Sensing ◽

Bacterial Quorum

Macrolide antibiotics are used in treating Pseudomonas aeruginosa chronic biofilm infections despite their unsatisfactory antibacterial activity, because they display several special activities, such as modulation of the bacterial quorum sensing and immunomodulatory effects on the host. In this study, we investigated the effects of the newly synthesized P. aeruginosa quorum-sensing autoinducer analogs (AIA-1, -2) on the activity of azithromycin and clarithromycin against P. aeruginosa. In the killing assay of planktonic cells, AIA-1 and -2 enhanced the bactericidal ability of macrolides against P. aeruginosa PAO1; however, they did not affect the minimum inhibitory concentrations of macrolides. In addition, AIA-1 and -2 considerably improved the killing activity of azithromycin and clarithromycin in biofilm cells. The results indicated that AIA-1 and -2 could affect antibiotic tolerance. Moreover, the results of hydrocarbon adherence and cell membrane permeability assays suggested that AIA-1 and -2 changed bacterial cell surface hydrophobicity and accelerated the outer membrane permeability of the hydrophobic antibiotics such as azithromycin and clarithromycin. Our study demonstrated that the new combination therapy of macrolides and AIA-1 and -2 may improve the therapeutic efficacy of macrolides in the treatment of chronic P. aeruginosa biofilm infections.

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Heterologous Inducible Expression ofEnterococcus faecalis pCF10 Aggregation Substance Asc10 inLactococcus lactis and Streptococcus gordonii Contributes to Cell Hydrophobicity and Adhesion to Fibrin

Journal of Bacteriology ◽

10.1128/jb.182.8.2299-2306.2000 ◽

2000 ◽

Vol 182 (8) ◽

pp. 2299-2306 ◽

Cited By ~ 54

Author(s):

Helmut Hirt ◽

Stanley L. Erlandsen ◽

Gary M. Dunny

Keyword(s):

Cell Surface ◽

Cell Surface Hydrophobicity ◽

Regulatory System ◽

Surface Hydrophobicity ◽

Bacterial Cells ◽

Streptococcus Gordonii ◽

Cell Surfaces ◽

Gene Encoding ◽

Aggregation Substance

ABSTRACT Aggregation substance proteins encoded by the sex pheromone plasmid family of Enterococcus faecalis have been shown previously to contribute to the formation of a stable mating complex between donor and recipient cells and have been implicated in the virulence of this increasingly important nosocomial pathogen. In an effort to characterize the protein further, prgB, the gene encoding the aggregation substance Asc10 on pCF10, was cloned in a vector containing the nisin-inducible nisA promoter and its two-component regulatory system. Expression of aggregation substance after nisin addition to cultures of E. faecalis and the heterologous bacteria Lactococcus lactis andStreptococcus gordonii was demonstrated. Electron microscopy revealed that Asc10 was presented on the cell surfaces ofE. faecalis and L. lactis but not on that ofS. gordonii. The protein was also found in the cell culture supernatants of all three species. Characterization of Asc10 on the cell surfaces of E. faecalis and L. lactisrevealed a significant increase in cell surface hydrophobicity upon expression of the protein. Heterologous expression of Asc10 on L. lactis also allowed the recognition of its binding ligand (EBS) on the enterococcal cell surface, as indicated by increased transfer of a conjugative transposon. We also found that adhesion of Asc10-expressing bacterial cells to fibrin was elevated, consistent with a role for the protein in the pathogenesis of enterococcal endocarditis. The data demonstrate that Asc10 expressed under the control of the nisA promoter in heterologous species will be an useful tool in the detailed characterization of this important enterococcal conjugation protein and virulence factor.

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Nitrofurantoin—Microbial Degradation and Interactions with Environmental Bacterial Strains

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16091526 ◽

2019 ◽

Vol 16 (9) ◽

pp. 1526 ◽

Cited By ~ 5

Author(s):

Amanda Pacholak ◽

Wojciech Smułek ◽

Agnieszka Zgoła-Grześkowiak ◽

Ewa Kaczorek

Keyword(s):

Environmental Impact ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Microbial Cell ◽

Continuous Exposure ◽

Bacterial Cells ◽

Bacterial Strains ◽

Nitrofuran Derivatives ◽

Living Organisms ◽

The Impact

The continuous exposure of living organisms and microorganisms to antibiotics that have increasingly been found in various environmental compartments may be perilous. One group of antibacterial agents that have an environmental impact that has been very scarcely studied is nitrofuran derivatives. Their representative is nitrofurantoin (NFT)—a synthetic, broad-spectrum antibiotic that is often overdosed. The main aims of the study were to: (a) isolate and characterize new microbial strains that are able to grow in the presence of NFT, (b) investigate the ability of isolates to decompose NFT, and (c) study the impact of NFT on microbial cell properties. As a result, five microbial species were isolated. A 24-h contact of bacteria with NFT provoked modifications in microbial cell properties. The greatest differences were observed in Sphingobacterium thalpophilum P3d, in which a decrease in both total and inner membrane permeability (from 86.7% to 48.3% and from 0.49 to 0.42 µM min−1) as well as an increase in cell surface hydrophobicity (from 28.3% to 39.7%) were observed. Nitrofurantoin removal by selected microbial cultures ranged from 50% to 90% in 28 days, depending on the bacterial strain. Although the isolates were able to decompose the pharmaceutical, its presence significantly affected the bacterial cells. Hence, the environmental impact of NFT should be investigated to a greater extent.

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Rhamnolipid-Induced Removal of Lipopolysaccharide from Pseudomonas aeruginosa: Effect on Cell Surface Properties and Interaction with Hydrophobic Substrates

Applied and Environmental Microbiology ◽

10.1128/aem.66.8.3262-3268.2000 ◽

2000 ◽

Vol 66 (8) ◽

pp. 3262-3268 ◽

Cited By ~ 293

Author(s):

Ragheb A. Al-Tahhan ◽

Todd R. Sandrin ◽

Adria A. Bodour ◽

Raina M. Maier

Keyword(s):

Pseudomonas Aeruginosa ◽

Cell Surface ◽

Structural Changes ◽

Fatty Acid Content ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Cell Surface Hydrophobicity ◽

Bacterial Cells ◽

Low Levels ◽

Rhamnolipid Biosurfactant

ABSTRACT Little is known about the interaction of biosurfactants with bacterial cells. Recent work in the area of biodegradation suggests that there are two mechanisms by which biosurfactants enhance the biodegradation of slightly soluble organic compounds. First, biosurfactants can solubilize hydrophobic compounds within micelle structures, effectively increasing the apparent aqueous solubility of the organic compound and its availability for uptake by a cell. Second, biosurfactants can cause the cell surface to become more hydrophobic, thereby increasing the association of the cell with the slightly soluble substrate. Since the second mechanism requires very low levels of added biosurfactant, it is the more intriguing of the two mechanisms from the perspective of enhancing the biodegradation process. This is because, in practical terms, addition of low levels of biosurfactants will be more cost-effective for bioremediation. To successfully optimize the use of biosurfactants in the bioremediation process, their effect on cell surfaces must be understood. We report here that rhamnolipid biosurfactant causes the cell surface ofPseudomonas spp. to become hydrophobic through release of lipopolysaccharide (LPS). In this study, two Pseudomonas aeruginosa strains were grown on glucose and hexadecane to investigate the chemical and structural changes that occur in the presence of a rhamnolipid biosurfactant. Results showed that rhamnolipids caused an overall loss in cellular fatty acid content. Loss of fatty acids was due to release of LPS from the outer membrane, as demonstrated by 2-keto-3-deoxyoctonic acid and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and further confirmed by scanning electron microscopy. The amount of LPS loss was found to be dependent on rhamnolipid concentration, but significant loss occurred even at concentrations less than the critical micelle concentration. We conclude that rhamnolipid-induced LPS release is the probable mechanism of enhanced cell surface hydrophobicity.

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Histological assessment, anti-quorum sensing, and anti-biofilm activities of Dioon spinulosum extract: in vitro and in vivo approach

Scientific Reports ◽

10.1038/s41598-021-03953-x ◽

2022 ◽

Vol 12 (1) ◽

Author(s):

Engy Elekhnawy ◽

Walaa A. Negm ◽

Mona El-Aasr ◽

Amal Abo Kamer ◽

Mohammed Alqarni ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Biofilm Formation ◽

Cell Surface Hydrophobicity ◽

Fibroblast Cell ◽

Surface Hydrophobicity ◽

Human Skin Fibroblast ◽

Electron Microscopes

AbstractPseudomonas aeruginosa is an opportunistic bacterium causing several health problems and having many virulence factors like biofilm formation on different surfaces. There is a significant need to develop new antimicrobials due to the spreading resistance to the commonly used antibiotics, partly attributed to biofilm formation. Consequently, this study aimed to investigate the anti-biofilm and anti-quorum sensing activities of Dioon spinulosum, Dyer Ex Eichler extract (DSE), against Pseudomonas aeruginosa clinical isolates. DSE exhibited a reduction in the biofilm formation by P. aeruginosa isolates both in vitro and in vivo rat models. It also resulted in a decrease in cell surface hydrophobicity and exopolysaccharide quantity of P. aeruginosa isolates. Both bright field and scanning electron microscopes provided evidence for the inhibiting ability of DSE on biofilm formation. Moreover, it reduced violacein production by Chromobacterium violaceum (ATCC 12,472). It decreased the relative expression of 4 quorum sensing genes (lasI, lasR, rhlI, rhlR) and the biofilm gene (ndvB) using qRT-PCR. Furthermore, DSE presented a cytotoxic activity with IC50 of 4.36 ± 0.52 µg/ml against human skin fibroblast cell lines. For the first time, this study reports that DSE is a promising resource of anti-biofilm and anti-quorum sensing agents.

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