Effects of some salts and sodicity on the growth of a Rhizobium leguminosarum bv. viceae strain isolated from a salt-affected soil

2001 ◽  
Vol 47 (9) ◽  
pp. 807-812 ◽  
Author(s):  
M Y Faituri ◽  
Y E El-Mahi ◽  
G A El-Hassan

The effects of sodium (Na+), calcium (Ca2+), magnesium (Mg2+), and boron (B) concentrations and sodicity, as measured by the sodium adsorption ratio (SAR), on the growth of a Rhizobium leguminosarum bv. viceae strain isolated from a salt-affected soil were studied. The rate of growth was measured in a yeast extract - mannitol broth, amended with salts having electrical conductivity (EC) of 4, 8, and 16 dS·m-1. Each salinity level was prepared to achieve SAR values of 10, 20, and 30 with or without graded B concentrations of 0.5, 1, 3, and 5 mg·L-1. We found that salinity levels equal to or more than 8 dS·m-1 had negative effects on Rhizobium growth during the first days of incubation, but the effects became less pronounced after 1 week. Na+ concentrations of more than 1.1 g·L-1 retarded growth, especially at high SAR values (i.e., at low Ca2+ concentrations). The retardation of growth increased with increases in EC up to 16 dS·m-1, at all sodicity levels. Mg2+ added together with Na+ or with Ca2+ + Na+ affected growth more negatively than Ca2+ + Na+ alone. The effect of Mg2+ became more pronounced with increased salinities and sodicities. It was concluded that EC of more than 4 dS·m-1 retarded growth of Rhizobium, but only at high sodicity levels. The relative specific ion effect on growth was in the order Na+ < Ca2+ < Mg2+. The harmful effect of Mg2+ on this strain was accentuated by adding Ca2+ to the cultural medium. When SAR increased from 10 to 30, Na+ had no clear effect on growth, irrespective of the accompanied cations, i.e, Ca2+, Mg2+, or Ca2+ + Mg2+. Growth was reduced by B concentrations as low as 0.5 mg·L-1, and the B effect was enhanced by increased salinity.Key words: Rhizobium leguminosarum bv. viceae, salinity, sodicity, boron.

1970 ◽  
Vol 17 ◽  
pp. 17-22 ◽  
Author(s):  
Kamal Singh ◽  
A. A. Khan ◽  
Iram Khan ◽  
Rose Rizvi ◽  
M. Saquib

Plant growth, yield, pigment and protein content of cow-pea were increased significantly at lower levels (20 and 40%) of fly ash but reverse was true at higher levels (80 and 100%). Soil amended by 60% fly ash could cause suppression in growth and yield in respect to 40% fly ash treated cow-pea plants but former was found at par with control (fly ash untreated plants). Maximum growth occurred in plants grown in soil amended with 40% fly ash. Nitrogen content of cow-pea was suppressed progressively in increasing levels of fly ash. Moreover,  Rhizobium leguminosarum  influenced the growth and yield positively but Meloidogyne javanica caused opposite effects particularly at 20 and 40% fly ash levels. The positive effects of R. leguminosarum were marked by M. javanica at initial levels. However, at 80 and 100% fly ash levels, the positive and negative effects of R. leguminosarum and/or M. javanica did not appear as insignificant difference persist among such treatments.Key words:  Meloidogyne javanica; Rhizobium leguminosarum; Fly ash; Growth; YieldDOI: 10.3126/eco.v17i0.4098Ecoprint An International Journal of Ecology Vol. 17, 2010 Page: 17-22 Uploaded date: 28 December, 2010  


Author(s):  
Bjorn Lous ◽  
Johan Graafland

AbstractLiterature has established that, on a macroeconomic level, income inequality has a negative effect on average life satisfaction. An unresolved question is, however, which income groups are harmed by income inequality. In this paper we investigate this relationship at the microeconomic level combining national indicators of income inequality with individual data of life satisfaction from the World Values Survey for 39 countries over a period of 25 years. Tests on moderation by income category show that the Gini coefficient is most negatively related to life satisfaction of the lowest income groups, but the negative effects also extends to other income groups. For the income share of the top 1% we find a similar result. These findings show that income inequality is especially a concern for the lower income groups, but that the harmful effect of income inequality also spillovers to the life satisfaction of other income groups.


2019 ◽  
Vol 70 (1) ◽  
pp. 59-67
Author(s):  
Anna Lenart-Boroń ◽  
Tadeusz Zając ◽  
Piotr Mateusz Boroń ◽  
Agnieszka Klimek-Kopyra

SummaryThe bacterial nodulation (nod) genes are essential in the formation process of root nodules. This study was aimed to verify the occurrence of nodule-associated bacteria in two pea varieties (“Tarchalska” and “Klif ”) inoculated withRhizobiuminoculants – Nitragine™ and a noncommercial one produced by the Polish Institute of Soil Science and Plant Cultivation (IUNG). The number of colonies isolated on yeast extract mannitol (YEM) agar from the nodules of “Klif ” inoculated with IUNG inoculants was significantly higher than the number of colonies isolated from other variants. Species identification was based on sequencing of 16S rDNA, which revealed that despite careful sterilization of nodules, sequences of other bacterial species were detected. Among them, one sequence belonged toRhizobium leguminosarum(isolated from IUNG inoculant). To assess the presence of nodulation-capableRhizobium, amplification of thenodCgene was performed, which revealed that of 29 samples, 19 were positive. The remaining isolates, including reference strain and bacteria isolated from Nitragine™, lacked this gene. The results show that pea nodules harbor a very diverse community of bacteria. The lack ofnodCgene in some strains isolated from plants inoculated with Nitragine™ and with IUNG inoculant proves that even ifR. leguminosarumare abundant, they may not be efficient in nodulation.


2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Siamak Shirani Bidabadi 1 ◽  
Majid Masoumian 2

To investigate the possible role of arbuscular mycrrhizal fungi (AMF) in alleviating the negative effects of salinity on Stevia rebaudiana (Bert.), the regenerated plantlets in tissue culture was transferred to pots in greenhouse and inoculated with Glomus intraradices. Salinity caused a significant decrease in chlorophyll content, photosynthesis efficiency and enhanced the electrolyte leakage. The use of AMF in salt –affected plants resulted in improved all above mentioned characteristics. Hydrogen peroxide and malondialdehyde (MDA) contents increased in salt stressed plants while a reduction was observed due to AMF inoculation. CAT activity showed a significant increase up to 2 g/l and then followed by decline at 5 g/l NaCl in both AMF and non-AMF treated stevia, however, AMF inoculated plants maintained lower CAT activity at all salinity levels (2 and 5 g/l). Enhanced POX activities in salt- treated stevia plants were decreased by inoculation of plants with AMF. The addition of NaCl to stevia plants also resulted in an enhanced activity of SOD whilst, AMF plants maintained higher SOD activity at all salinity levels than those of non-AMF inoculated plants. AMF inoculation was capable of alleviating the damage caused by salinity on stevia plants by reducing oxidative stress and improving photosynthesis efficiency. 


2019 ◽  
pp. 1959-1966
Author(s):  
Francisco Romário Andrade Figueiredo ◽  
Anderson Carlos de Melo Gonçalves ◽  
João Everthon da Silva Ribeiro ◽  
Toshik Iarley da Silva ◽  
Jackson Silva Nóbrega ◽  
...  

Salinity is one of the modern agriculture major obstacles, causing several physiological disturbances in plants, adversely affecting its growth and development. Therefore, some techniques are required in order to alleviate the negative effects of salinity stress on plants, as for example an adequate nitrogen fertilization. The aim of this study was to assess the ecophysiological responses of sugar apple plants (Annona squamosa L.) submitted to different salinity levels and nitrogen fertilization doses. The experimental design was a randomized block in an incomplete factorial scheme, with five electrical conductivities of the irrigation water (ECw: 0.5, 1.01, 2.25, 3.49 and 4.0 dS m-1) and five nitrogen doses (0, 101, 350, 598.2 and 700 mg dm-3), with four replicates, generated from the Box Central Composite experimental design matrix. Through a daily course, it was observed variations in gas exchange, chlorophyll content and fluorescence parameters. The gas exchange, chlorophyll content and fluorescence parameters were measured on photosynthetically active leaves. There was significant interaction between the salinity levels (ECw) and nitrogen doses for the chlorophyll indexes. The electrical conductivity of the irrigation water caused significant negative effects on chlorophyll fluorescence. Therefore, it can be stated that the sugar apple plants ecophysiology varies through the day and that salinity affects its chlorophyll content and fluorescence.


Author(s):  
Ahmet Ali Koç ◽  
Oznur Ozdamar ◽  
Peyman Uysal

This paper aims to analyse the determinants of the food insecurity examining the relationship of important economic,social and natural factors with an overall food insecurity index that derived by dividing the food imports value to the sum of total exports and the net remittance inflows. Overall analysis is made by employing panel-data methods using a dataset that covers 18MENA countries and Turkey for the period of 1990-2014.Emprical results support the evidence of the harmful effect of price increases on food security. Furthermore, significance of education related variables and fresh water withdrawals indicate that the region has had benefit from nearly 30 years investment on education and intensive water exploitation, although poor water management and great waste of irrigation will most likely have negative effects on food security in the near future.


1986 ◽  
Vol 4 (2) ◽  
pp. 101-106 ◽  
Author(s):  
David J. H. Smith

Although gifted students might be expected to welcome school tests and examinations as a means of attaining positive rewards, it is argued that the characteristics of gifted students such as a preference for self-initiated, independent learning make them particularly vulnerable to negative effects associated with assessment. Features of school assessment which are likely to have a harmful effect upon the gifted student's attitudes and intrinsic motivation are identified and approaches are suggested for improving assessment procedures.


2021 ◽  
Vol 22 (1) ◽  
pp. 6-13
Author(s):  
Rini Andriani

The gadget utilization among preschoolers in connection with technological advances is known to have positive and negative effects on growth and development. This study aims to identify the overview of gadget utilization and its effect on growth and development in preschool children. An analytic survey with a cross-sectional design was conducted by filling out questionnaires carried out by parents. The anthropometric measurements and developmental assessments were carried out using Indonesia Developmental Pre-screening Questionnaire (IDPQ). A hundred children and their parents participated in this study. It was found that 82% of parents lent their gadgets, and 89% taught their children to use gadgets. There were similarities in gadget utilization between parents and children, namely watching videos (73% and 90%) and communicating (92% and 40.4%). The duration of the gadget utilization by children was high, 3.7±2.2 hours/day. However, no significant correlation in the analysis between the duration of smartphone usage and nutritional status (p: 0.599). In children with different IDPQ values, there was no significant difference in the duration of device usage (p: 0.991). It can concluded the duration of the gadget utilization in children carried out by their parents did not affect the growth and development of the children.


2020 ◽  
Vol 12 (20) ◽  
pp. 8389
Author(s):  
Hassan-Sibroe A. Daanaa ◽  
Mennatullah Abdou ◽  
Hanan A. Goda ◽  
Mohamed T. Abbas ◽  
Mervat A. Hamza ◽  
...  

Although plant-based culture media enhances in vitro cultivation of rhizobacteria, studies assessing their biomass potential for large-scale applications are lacking. Here, we advance plant pellets (PPs) as a novel technology to unlock the potential of such vegan culture media for biomass production of Rhizobium leguminosarum. PP formulations were based on mixtures of Egyptian clover powder and the agro-byproducts glycerol and molasses. These mixtures were either contained or not contained in teabags during culture media preparation. Metrics of biomass included colony forming units, optical density (OD600nm), and cell dry weight (DW). Biomass comparisons between culture media based on PPs and standard yeast extract mannitol (YEM) revealed that the following PPs composition, contained in teabags, cultivated rhizobia at levels comparable to YEM: 16 g clover powder, 5% molasses, and 0.8% glycerol. This PPs composition enabled shorter generation times of rhizobia (PP: 3.83 h, YEM: 4.28 h). Strikingly, PPs mixtures supplemented with 10% molasses and not contained in teabags promoted rhizobia without apparent lag phases and produced 25% greater DW than YEM. PPs potentiate the use of dehydrated vegan feedstocks for both plant microbiota cultivation and biomass production and appear as cost- and labor-effective tools, easy to handle and store for plant-based culture media preparation.


1972 ◽  
Vol 35 (5) ◽  
pp. 269-275
Author(s):  
M. C. Goel ◽  
E. H. Marth

Cobalt chloride, manganous chloride, ferric citrate, and ferrous sulfate were tested for their effects on growth of and chain formation by four strains of Leuconostoc citrovorum. Chemicals were added to sterile skimmilk before inoculation with test cultures. Numbers of L. citrovorum were determined with plate count procedures at periodic intervals during 30 hr of incubation at 22 or 30 C. Smears made concurrently were stained and examined microscopically to determine numbers of cells in chains. Cobalt chloride at a concentration of 0.1% completely inhibited growth of all strains of L. citrovorum, whereas 0.01% retarded growth of three strains, and 0.001% was essentially without effect. Chains of L. citrovorum strains exposed to 0.1% cobalt chloride remained fairly constant in length throughout the incubation, probably because the organism failed to grow. Lower concentrations of cobalt chloride had no marked effects on chain length of L. citrovorum. Manganous chloride (0.00001–0.001%), ferric citrate (0.001–0.1%), and ferrous sulfate (0.001–0.1%) were essentially without effect on growth of most L. citrovorum strains at 22 or 30 C. One strain produced markedly longer chains in the presence of manganous chloride, whereas others behaved similarly but less dramatically. Ferric citrate at the higher concentrations caused somewhat longer chains to be formed when L. citrovorum grew at either 22 or 30 C. The organism also tended to form longer chains when skimmilk contained 0.01 or 0.1% ferrous sulfate. This chemical also markedly reduced the generation time of L. citrovorum when incubation was at 22 C.


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