SOLUBILITY MEASUREMENTS OF TETRAGONAL LYSOZYME CRYSTALS

2006 ◽  
Vol 20 (25n27) ◽  
pp. 4117-4122 ◽  
Author(s):  
YOSHIHISA SUZUKI ◽  
ATSUTO ARAI ◽  
KATSUHIRO TAMURA
Keyword(s):  
Growth Rates ◽  
The Difference ◽  
Lysozyme Crystals ◽  
Lysozyme Crystal

Solubility of tetragonal lysozyme crystal was measured by using two different methods. The solubility measured by using growth rates of the crystal corresponded well to that measured by using change in the concentration of the supernatant of the solution. Our data also corresponded well to the data obtained by Sazaki et al. and Gray et al., while not to those obtained by Howard et al. and Rosenberger et al. The discrepancies are due to the difference in the principles of the methods. We also checked effects of different initial concentrations on the solubility. Contrary to previous reports, the solutions of the different initial concentrations did not reach equilibrium when their concentrations attained to a same value.

scholarly journals The Study of the Mechanism of Protein Crystallization in Space by Using Microchannel to Simulate Microgravity Environment

Crystals ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 400 ◽  
Author(s):  
Yong Yu ◽  
Kai Li ◽  
Hai Lin ◽  
Ji-Cheng Li
Keyword(s):  
Growth Rates ◽  
Simulated Microgravity ◽  
Crystal Surface ◽  
Protein Crystallization ◽  
Experimental Results ◽  
Protein Crystals ◽  
Microgravity Environment ◽  
Microgravity Conditions ◽  
Lysozyme Crystals ◽  
Lysozyme Crystal

Space is expected to be a convection-free, quiescent environment for the production of large-size and high-quality protein crystals. However, the mechanisms by which the diffusion environment in space improves the quality of the protein crystals are not fully understood. The interior of a microfluidic device can be used to simulate a microgravity environment to investigate the protein crystallization mechanism that occurs in space. In the present study, lysozyme crystals were grown in a prototype microchannel device with a height of 50 μm in a glass-polydimethylsiloxane (PDMS)-glass sandwich structure. Comparative experiments were also conducted in a sample pool with a height of 2 mm under the same growth conditions. We compared the crystal morphologies and growth rates of the grown crystals in the two sample pools. The experimental results showed that at very low initial supersaturation, the morphology and growth rates of lysozyme crystals under the simulated microgravity conditions is similar to that on Earth. With increasing initial supersaturation, a convection-free, quiescent environment is better for lysozyme crystal growth. When the initial supersaturation exceeded a threshold, the growth of the lysozyme crystal surface under the simulated microgravity conditions never completely transform from isotropic to anisotropic. The experimental results showed that the convection may have a dual effect on the crystal morphology. Convection can increase the roughness of the crystal surface and promote the transformation of the crystal form from circular to tetragonal during the crystallization process.

Effects of turbidity on prey consumption and growth in brook trout and implications for bioenergetics modeling

2001 ◽  
Vol 58 (2) ◽  
pp. 386-393 ◽  
Author(s):  
John A Sweka ◽  
Kyle J Hartman
Keyword(s):  
Growth Rates ◽  
Brook Trout ◽  
Specific Growth ◽  
Abiotic Factors ◽  
Foraging Strategies ◽  
Turbid Water ◽  
Daily Consumption ◽  
Consumption Rates ◽  
The Difference ◽  
Specific Growth Rates

Brook trout (Salvelinus fontinalis) were held in an artificial stream to observe the influence of turbidity on mean daily consumption and specific growth rates. Treatment turbidity levels ranged from clear (<3.0 nephelometric turbidity units (NTU)) to very turbid water (> 40 NTU). Observed mean daily specific consumption rates were standardized to the mean weight of all brook trout tested. Turbidity had no significant effect on mean daily consumption, but specific growth rates decreased significantly as turbidity increased. Brook trout in turbid water became more active and switched foraging strategies from drift feeding to active searching. This switch was energetically costly and resulted in lower specific growth rates in turbid water as compared with clear water. Bioenergetics simulations were run to compare observed growth with that predicted by the model. Observed growth values fell below those predicted by the model and the difference increased as turbidity increased. Abiotic factors, such as turbidity, which bring about changes in the activity rates of fish, can have implications for the accuracy of predicted growth by bioenergetics models.

The kinetics of Ge lateral overgrowth on SiO2

MRS Advances ◽  
2015 ◽  
Vol 1 (23) ◽  
pp. 1703-1708 ◽  
Author(s):  
M. Yako ◽  
N. J. Kawai ◽  
Y. Mizuno ◽  
K. Wada
Keyword(s):  
Growth Kinetics ◽  
Growth Rates ◽  
Growth Process ◽  
Theoretical Calculations ◽  
Film Formation ◽  
Lateral Overgrowth ◽  
Flat Film ◽  
The Difference ◽  
Kinetics Of

ABSTRACTThe kinetics of Ge lateral overgrowth on SiO2 with line-shaped Si seeds is examined. The growth process is described by the difference between the growth rates of Ge on (100) planes (GR100) and <311> facets (GR311). The theoretical calculations well reproduce the growth kinetics. It is shown that narrowing the line-seeds helps Ge coalescence and flat film formation.

scholarly journals Quasi-biennial and quasi-triennial oscillations in the growth rates of atmospheric chlorofluorocarbons 11 and 12

MAUSAM ◽  
2022 ◽  
Vol 53 (3) ◽  
pp. 349-358
Author(s):  
R. P. KANE
Keyword(s):  
Southern Hemisphere ◽  
Growth Rates ◽  
Southern Oscillation ◽  
Spectral Characteristics ◽  
Linear Increase ◽  
Transport Processes ◽  
Emission Rates ◽  
Low Latitude ◽  
The Difference ◽  
Prominent Peak

The 12-monthly running means of CFC-11 and CFC-12 were examined for 1977-1992. As observed by earlier workers, during 1977-1988, there was a rapid, almost linear increase of these compounds, ~70% in the northern and ~77% in the southern hemisphere. From 1988 up to 1992, growth rates were slower, more so for CFC-11 in the northern hemisphere. Superposed on this pattern were QBO, QTO (Quasi-Biennial and Quasi-Triennial Oscillations). A spectral analysis of the various series indicated the following. The 50 hPa low latitude zonal wind had one prominent QBO peak at 2.58 years and much smaller peaks at 2.00 (QBO) and 5.1 years. The Southern oscillation index represented by (T-D), Tahiti minus Darwin atmospheric pressure, had a prominent peak at 4.1 years and a smaller peak at 2.31 years. CFC-11 had only one significant peak at 3.7 years in the southern hemisphere, roughly similar to the 4.1 year (T-D) peak. CFC-12 had prominent QBO (2.16-2.33 years) in both the hemispheres and a QTO (3.6 years) in the southern hemisphere. For individual locations, CFC-11 showed barely significant QBO in the range (1.95-3.07 years), while CFC 12 showed strong QBO in the range (1.86-2.38 years). The difference in the spectral characteristics of CFC-11 and CFC 12 time series is attributed to differences in their lifetimes (44 and 180 years), source emission rates and transport processes.

scholarly journals Seasonal patterns of pasture production in the Bay of Plenty and Waikato

1991 ◽  
pp. 67-72
Author(s):  
J.A. Baars ◽  
G.J. Goold ◽  
M.F. Hawke ◽  
P.J. Kilgarriff ◽  
M.D. Rolm
Keyword(s):  
Growth Rates ◽  
Early Spring ◽  
Early Summer ◽  
Growth Patterns ◽  
Climatic Data ◽  
Large Contribution ◽  
Pasture Production ◽  
Summer Peak ◽  
The Difference

Patterns of pasture growth were measured on 3 farms in the Bay of Plenty (BOP) and at No2 Dairy (Ruakura Agricultural Centre) in the Waikato from 1989 to 199 1. A standardised trim technique with cages and 4-weekly cutting under grazing was used. Long-term seasonal growth patterns, using a predictive pasture model, were also simulated. Simulated pasture growth from long-term climatic data shows that pasture growth rates are higher in winter, early spring and late autumn in the BOP than the Waikato. However, the actual measurements over the 2 years show that pasture growth over the latter periods is lower at the BOP sites than at the Waikato site. In the BOP the spring peak is much later than in the Waikato while an early summer peak, with higher growth rates than in the Waikato, occurred in the BOP. No such summer peak was evident in the Waikato. The difference between the two regions is caused by the large contribution of subtropical grasses to sward production in summer and autumn, The prolific summer growth of subtropical grasses may explain the low ryegrass content and low pasture production in winter. The lower than expected autumn, winter, spring production may also becaused by low clover content, possibly a result of competition from subtropical grasses and a sulphur deficiency. The apparent low amount of nitrogen fixed by clover may explain the low rates of pasture production over the cooler season. Applications of nitrogen fertiliser may substantially increase dry matter production from April to September. Keywords pasture,simulation,subtropical grasses, Paspalum, Digitaria sanguinalis, growth rates

The Relative Growth of Parts in Palaemon Carcinus

1930 ◽  
Vol 7 (2) ◽  
pp. 165-174
Author(s):  
M. A. TAZELAAR
Keyword(s):  
Growth Rate ◽  
Growth Rates ◽  
Linear Measurements ◽  
Relative Growth ◽  
Male And Female ◽  
Relative Growth Rates ◽  
Rate Of Growth ◽  
The Difference ◽  
Rates Of Growth

Linear measurements of certain appendages and the carapace of P. carcinus were made and plotted in various ways. The following conclusions were drawn: 1. The cheliped shows heterogonic growth in both male and female, but more markedly in the male, the values of k being: male 1.8 and female 1.48 2. The pereiopods in both male and female are slightly heterogonic. The relative growth rates are graded from p3 to p5, that of p3 being slightly greater than that of p5 3. Of the ordinary pereiopods the rate of growth of p1 is the smallest in the male, but the largest in the female. 4. The difference between the rates of growth of p1 and p3 in male and female is greatest where the rate of growth in the heterogonic organ, the cheliped, is most excessive in the male. 5. The growth of the 3rd maxilliped is slightly negatively heterogonic, the value of k in the male being 0.93 and in the female 0.95. Hence there seems to be a correlation between the marked heterogony in the cheliped on the growth rate of neighbouring appendages. In those immediately posterior to the cheliped the growth rate is increased and in those anterior decreased.

Group I Clostridium botulinum Strains Show Significant Variation in Growth at Low and High Temperatures

2009 ◽  
Vol 72 (2) ◽  
pp. 375-383 ◽  
Author(s):  
KATJA HINDERINK ◽  
MIIA LINDSTRÖM ◽  
HANNU KORKEALA
Keyword(s):  
Growth Rates ◽  
Significant Variation ◽  
Clostridium Botulinum ◽  
Maximum Growth ◽  
Type B ◽  
Strain Variation ◽  
Optimum Growth Temperature ◽  
Group I ◽  
Botulinum Type ◽  
The Difference

The minimum and maximum growth temperatures of 23 group I Clostridium botulinum strains of the toxin types A, AB, B, and F were determined. Moreover, the maximum growth rates at 20, 37, and 42°C of the same strains were recorded. The minimum growth temperatures varied from 12.8 to 16.5°C, whereas the maximum growth temperatures showed even wider variation, from 40.9 to 48.0°C. At 20 and 37°C, a twofold difference in maximum growth rates between the slowest and the fastest growing strains was found; at 42°C the difference was more than 30-fold. As expected, all strains grew significantly slower at 20°C than at 37°C. However, eight type B strains grew substantially faster at 42°C than they did at 37°C. These findings indicate that the optimum growth temperature for some group I C. botulinum type B strains is higher than the temperature of 37°C that is generally accepted. A significant correlation between maximum growth rates at 42°C and maximum growth temperatures was found for type B and F strains, whereas for type A strains no such correlation could be found. Strain variation was particularly high for the type B strains, reflecting the wide genetic diversity of this toxin type. The significant variation between strains of group I C. botulinum may have an impact on inoculation studies and predictive modeling when assessing the safety of foods.

Age, Growth, and Sexual Maturity of the Shorthorn Sculpin, Myoxocephalus scorpius, in Newfoundland Waters

1970 ◽  
Vol 27 (12) ◽  
pp. 2155-2158 ◽  
Author(s):  
G. P. Ennis
Keyword(s):  
Growth Rates ◽  
Sexual Maturity ◽  
Average Length ◽  
Maximum Size ◽  
Age Group ◽  
Younger Age ◽  
Shorthorn Sculpin ◽  
Males And Females ◽  
The Difference ◽  
Myoxocephalus Scorpius

In Newfoundland waters, shorthorn sculpins, Myoxocephalus scorpius (L.), live to age 15 and attain a maximum size of just over 50 cm. The growth rates of males and females are little different below age 4, but above age 4 the females grow faster than the males, and the difference between average length-at-age for males and females gets progressively larger with age. Males mature at a younger age and at a smaller size than females. In any age-group where there are mature and immature individuals the mature ones are larger.

scholarly journals Growth and metabolism in the Antarctic brachiopod Liothyrella uva

1997 ◽  
Vol 352 (1355) ◽  
pp. 851-858 ◽  
Author(s):  
Lloyd S. Peck ◽  
Simon Brockington ◽  
Thomas Brey
Keyword(s):  
Growth Rates ◽  
Marine Invertebrates ◽  
Shell Growth ◽  
Growth Study ◽  
Tissue Mass ◽  
Phytoplankton Productivity ◽  
Main Period ◽  
The Difference ◽  
The Antarctic ◽  
Mass Increase

Summer and winter growth rates were assessed separately for a population of the Antarctic brachiopod Liothyrella uva between early January 1992 and December 1993. Annual shell growth rates (1.6–2.3 mm yr −1 for a 5 mm individual; 0.96–1.44 mm −1 for a 20 mm specimen) were two to six times slower than those reported for temperate species. Growth in specimens less than 20 mm in length was faster in 1992 than in 1993, although differences between years over the whole size range were not significant. Surprisingly, growth was much faster in winter periods than during the summers. A 5 mm long individual grew five times faster in winter than in summer, and for a 20 mm long specimen the difference was 13 times. This runs contrary to current ideas on the effects of seasonality on the biology of polar marine invertebrates, but may be an effect of maximizing the efficiency of resource utilization. Comparisons with previous work showed shell growth to be decoupled from periods of tissue mass increase, and also from the main period of phytoplankton productivity. Oxygen consumption of 75 of the specimens used in the growth study was measured to test the hypothesis that basal metabolic rates should be inversely correlated with growth rates. Unexpectedly, an analysis of residuals produced no significant relationship, positive or negative, between growth rate and basal metabolism ( F = 1.37, p =0.25, n = 75).

Age, growth and sex determination in a population of smooth snakes, Coronella austriaca in southern England

2004 ◽  
Vol 25 (2) ◽  
pp. 137-150 ◽  
Author(s):  
Keyword(s):  
Sex Determination ◽  
Age Structure ◽  
Growth Rates ◽  
Growth Curves ◽  
Tail Length ◽  
Daily Growth ◽  
Southern England ◽  
The Difference ◽  
The Relationship ◽  
Coronella Austriaca

AbstractBetween 1992-2001 a study of individually marked smooth snakes Coronella austriaca was done in Wareham Forest, southern England. The sex of individual snakes was determined using the relationship between tail length and snout-vent length, and the presence/absence of a hemi-penes swelling at the base of the tail. Males had longer tails than females at all ages/sizes and the difference between the sexes increased with increasing snout-vent length. Using multiple captures of known individuals over many years, size related daily growth rates were determined for each sex that enabled growth curves to be produced. The age structure of the population was determined for each complete year of the study. Very few young small snakes were captured each year. Of all the females who were potentially able to breed, only the oldest, and therefore largest, did so.

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