scholarly journals Virgin Coconut Oil Supplementation Prevents Bone Loss in Osteoporosis Rat Model

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Zil Hayatullina ◽  
Norliza Muhammad ◽  
Norazlina Mohamed ◽  
Ima-Nirwana Soelaiman
Keyword(s):  
Bone Loss ◽  
Rat Model ◽  
Bone Structure ◽  
Bone Microarchitecture ◽  
Coconut Oil ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Antioxidant Compounds ◽  
Virgin Coconut Oil ◽  
The Right

Oxidative stress and free radicals have been implicated in the pathogenesis of osteoporosis. Therefore, antioxidant compounds have the potential to be used in the prevention and treatment of the disease. In this study, we investigated the effects of virgin coconut oil (VCO) on bone microarchitecture in a postmenopausal osteoporosis rat model. VCO is a different form of coconut oil as it is rich with antioxidants. Three-month-old female rats were randomly grouped into baseline, sham-operated, ovariectomized control (Ovx), and ovariectomized rats fed with 8% VCO in their diet for six weeks (Ovx+VCO). Bone histomorphometry of the right femora was carried out at the end of the study. Rats supplemented with VCO had a significantly greater bone volume and trabecular number while trabecular separation was lower than the Ovx group. In conclusion, VCO was effective in maintaining bone structure and preventing bone loss in estrogen-deficient rat model.

scholarly journals Two Different Isomers of Vitamin E Prevent Bone Loss in Postmenopausal Osteoporosis Rat Model

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Norliza Muhammad ◽  
Douglas Alwyn Luke ◽  
Ahmad Nazrun Shuid ◽  
Norazlina Mohamed ◽  
Ima-Nirwana Soelaiman
Keyword(s):  
Free Radicals ◽  
Vitamin E ◽  
Bone Loss ◽  
Rat Model ◽  
Bone Microarchitecture ◽  
The Body ◽  
Alpha Tocopherol ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Prevent Bone Loss

Postmenopausal osteoporotic bone loss occurs mainly due to cessation of ovarian function, a condition associated with increased free radicals. Vitamin E, a lipid-soluble vitamin, is a potent antioxidant which can scavenge free radicals in the body. In this study, we investigated the effects of alpha-tocopherol and pure tocotrienol on bone microarchitecture and cellular parameters in ovariectomized rats. Three-month-old female Wistar rats were randomly divided into ovariectomized control, sham-operated, and ovariectomized rats treated with either alpha-tocopherol or tocotrienol. Their femurs were taken at the end of the four-week study period for bone histomorphometric analysis. Ovariectomy causes bone loss in the control group as shown by reduction in both trabecular volume (BV/TV) and trabecular number (Tb.N) and an increase in trabecular separation (Tb.S). The increase in osteoclast surface (Oc.S) and osteoblast surface (Ob.S) in ovariectomy indicates an increase in bone turnover rate. Treatment with either alpha-tocopherol or tocotrienol prevents the reduction in BV/TV and Tb.N as well as the increase in Tb.S, while reducing the Oc.S and increasing the Ob.S. In conclusion, the two forms of vitamin E were able to prevent bone loss due to ovariectomy. Both tocotrienol and alpha-tocopherol exert similar effects in preserving bone microarchitecture in estrogen-deficient rat model.

scholarly journals The Effects ofCosmos caudatuson Structural Bone Histomorphometry in Ovariectomized Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Norazlina Mohamed ◽  
Sharon Gwee Sian Khee ◽  
Ahmad Nazrun Shuid ◽  
Norliza Muhammad ◽  
Farihah Suhaimi ◽  
...  
Keyword(s):  
Bone Histomorphometry ◽  
Therapeutic Agent ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Antioxidant Compounds ◽  
Protective Agent ◽  
Cosmos Caudatus ◽  
Bone Damage ◽  
Trabecular Separation ◽  
Trabecular Number

Osteoporosis is considered a serious debilitating disease.Cosmos caudatus(ulam raja), a plant containing antioxidant compounds and minerals, may be used to treat and prevent osteoporosis. This study determines the effectiveness ofC. caudatusas bone protective agent in postmenopausal osteoporosis rat model. Thirty-two female rats, aged 3 months old, were divided into 4 groups. Group one was sham operated (sham) while group two was ovariectomized. These two groups were given ionized water by forced feeding. Groups three and four were ovariectomized and given calcium 1% ad libitum and force-fed withC. caudatusat the dose of 500 mg/kg, respectively. Treatments were given six days per week for a period of eight weeks. Body weight was monitored every week and structural bone histomorphometry analyses of the femur bones were performed. Ovariectomy decreased trabecular bone volume (BV/TV), decreased trabecular number (Tb.N), and increased trabecular separation (Tb.Sp). Both calcium 1% and 500 mg/kgC. caudatusreversed the above structural bone histomorphometric parameters to normal level.C. caudatusshows better effect compared to calcium 1% on trabecular number (Tb.N) and trabecular separation (Tb.Sp). Therefore,Cosmos caudatus500 mg/kg has the potential to act as the therapeutic agent to restore bone damage in postmenopausal women.

scholarly journals PELATIHAN PEMBUATAN VIRGIN COCONUT OIL (VCO) BAGI SISWA SISWI SMK DAN SMA MUTIARA 17 AGUSTUS

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Rizky Arcinthya Rachmania ◽  
Elly Wardani
Keyword(s):  
High School Students ◽  
Community Service ◽  
Coconut Oil ◽  
Virgin Coconut Oil ◽  
Economic Activities ◽  
School Students ◽  
The Family ◽  
Local Students ◽  
The Right ◽  
Marketing Implementation

<p>Adolescence is prone to deviations and impropriety should be grown a sense of creativity. Creativity of students to be skilled in making VCO so that will grow entrepreneurship spirit for students. Skills in the way of making VCO is expected to increase students' creativity and applied in economic activities to increase student income. The solution offered from the problems that have been mentioned above, then training the manufacture of Virgin Coconut Oil (VCO) is very necessary. Program activities offered in the form of socialization or introduction of VCO products produced both from the benefits and advantages they have compared with other similar products, and there is also a demonstration later in the making of VCO and explanation of how the right and proper marketing. Implementation of community service is specifically a promotive effort in the field of business development to create skilled and productive student students in producing a health product that can later be utilized both for themselves, the family and the surrounding community. Community service activities, also relevant to economics and business, where the product will be marketed so that this can be an income or additional income for local students. The participants' response to this counseling is quite high, indicated by the attendance rate of 80% of the number of invitations. Understanding high school students and SMK Mutiara to the benefits and content of VCO and how to manufacture the correct VCO using this simple and cheap enzymatic method was responded with great enthusiasm by the participants.</p>

scholarly journals Short- and long-term effects of calcium and exercise on bone mineral density in ovariectomized rats

2001 ◽  
Vol 86 (4) ◽  
pp. 521-527 ◽  
Author(s):  
Joseé Gala ◽  
Manuel Di´az-curiel ◽  
Concepcioó de la Piedra ◽  
Jesu´s Calero
Keyword(s):  
Bone Mineral Density ◽  
Bone Loss ◽  
Bone Mineral ◽  
Exercise Programme ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Standard Diet ◽  
Mineral Density ◽  
Left Femur ◽  
Ovx Rats

At the level of prevention of bone mineral loss produced by ovariectomy, the aim of the present study was to determine the effect produced by supplementation of Ca in the diet and a moderate exercise programme (treadmill), simultaneously or separately, in ovariectomized rats, an experimental model of postmenopausal bone loss. Female Wistar rats (n110, 15 weeks old) were divided into five groups: (1) OVX, rats ovariectomized at 15 weeks of age, fed a standard diet; (2) SHAM, rats sham operated at 15 weeks of age, fed a standard diet; (3) OVX–EX, ovariectomized rats, fed a standard diet and performing the established exercise programme; (4) OVX–Ca, ovariectomized rats fed a diet supplemented with Ca; (5) OVX–EXCa, ovariectomized rats with the exercise programme and diet supplemented with Ca. The different treatments were initiated 1 week after ovariectomy and were continued for 13 weeks for subgroup 1 and 28 weeks for subgroup 2, to look at the interaction of age and time passed from ovariectomy on the treatments. Bone mineral density (BMD) was determined, at the end of the study, in the lumbar spine (L2, L3 and L4) and in the left femur using a densitometer. Bone turnover was also estimated at the end of the study, measuring the serum formation marker total alkaline phosphatase (AP) and the resorption marker serum tartrate-resistant acid phosphatase (TRAP). As expected, OVX rats showed a significant decrease (P<0·05) in BMD, more pronounced in subgroup 2, and a significant increase in AP and TRAP with regard to their respective SHAM group. The simultaneous treatment with Ca and exercise produced the best effects on lumbar and femoral BMD of ovariectomized rats, partially avoiding bone loss produced by ovariectomy, although it was not able to fully maintain BMD levels of intact animals. This combined treatment produced a significant increase in AP, both in subgroups 1 and 2, and a decrease in TRAP in subgroup 1, with regard to OVX group. The exercise treatment alone was able to produce an increase in BMD with regard to OVX group only in subgroup 1 of rats (younger animals and less time from ovariectomy), but not in subgroup 2. In agreement with this, there was an increase of AP in both subgroups, lower than that observed in animals submitted to exercise plus Ca supplement, and a decrease of TRAP in subgroup 1, without significant changes in this marker in the older rats. Ca treatment did not produce any significant effect on BMD in OVX rats in both subgroups of animals, showing a decrease of AP and TRAP levels in the younger animals with no significant variations in markers of bone remodelling in the older female rats compared with their respective OVX group.

scholarly journals Preventive Effects of Collagen Peptide from Deer Sinew on Bone Loss in Ovariectomized Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
He Zhang ◽  
Ying Dong ◽  
Bin Qi ◽  
Li Liu ◽  
Guangxin Zhou ◽  
...  
Keyword(s):  
Body Weight ◽  
Bone Loss ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Uterine Weight ◽  
Mineral Density ◽  
Active Constituent ◽  
Collagen Peptide ◽  
Calcium Phosphorus ◽  
Serum Biochemical

Deer sinew (DS) has been used traditionally for various illnesses, and the major active constituent is collagen. In this study, we assessed the effects of collagen peptide from DS on bone loss in the ovariectomized rats. Wister female rats were randomly divided into six groups as follows: sham-operated (SHAM), ovariectomized control (OVX), OVX given 1.0 mg/kg/week nylestriol (OVX + N), OVX given 0.4 g/kg/day collagen peptide (OVX + H), OVX given 0.2 g/kg/day collagen peptide (OXV + M), and OVX given 0.1 g/kg/day collagen peptide (OXV + L), respectively. After 13 weeks of treatment, the rats were euthanized, and the effects of collagen peptide on body weight, uterine weight, bone mineral density (BMD), serum biochemical indicators, bone histomorphometry, and bone mechanics were observed. The data showed that BMD and concentration of serum hydroxyproline were significantly increased and the levels of serum calcium, phosphorus, and alkaline phosphatase were decreased. Besides, histomorphometric parameters and mechanical indicators were improved. However, collagen peptide of DS has no effect on estradiol level, body weight, and uterine weight. Therefore, these results suggest that the collagen peptide supplementation may also prevent and treat bone loss.

scholarly journals The Effects of Virgin Coconut Oil on Bone Oxidative Status in Ovariectomised Rat

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Mouna Abdelrahman Abujazia ◽  
Norliza Muhammad ◽  
Ahmad Nazrun Shuid ◽  
Ima Nirwana Soelaiman
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Rat Model ◽  
Coconut Oil ◽  
Antioxidant Property ◽  
Oxidative Status ◽  
Control Group ◽  
Normal Female ◽  
Sprague Dawley ◽  
Virgin Coconut Oil

Virgin coconut oil (VCO) was found to have antioxidant property due to its high polyphenol content. The aim of this study was to investigate the effect of the virgin coconut oil on lipid peroxidation in the bone of an osteoporotic rat model. Normal female Sprague-Dawley rats aged 3 months old were randomly divided into 4 groups, with 8 rats in each group: baseline, sham, ovariectomised (OVX) control group, and OVX given 8% VCO in the diet for six weeks. The oxidative status of the bone was assessed by measuring the index of lipid peroxidation, which is malondialdehyde (MDA) concentration, as well as the endogenous antioxidant enzymes glutathione peroxidase (GPX) and superoxide dismutase (SOD) in the tibia at the end of the study. The results showed that there was a significant decrease in MDA levels in the OVX-VCO group compared to control group. Ovariectomised rats treated with VCO also had significantly higher GPX concentration. The SOD level seemed to be increased in the OVX-VCO group compared to OVX-control group. In conclusion, VCO prevented lipid peroxidation and increased the antioxidant enzymes in the osteoporotic rat model.

Long-Term Therapy with a New Chemically Modified Tetracycline (CMT-8) Inhibits Bone Loss in Femurs of Ovariectomized Rats

1998 ◽  
Vol 12 (1) ◽  
pp. 76-81 ◽  
Author(s):  
T. Sasaki ◽  
N.S. Ramamurthy ◽  
L.M. Golub
Keyword(s):  
Bone Loss ◽  
Bone Formation ◽  
Trabecular Bone ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Term Therapy ◽  
Ovx Rats ◽  
Trabecular Bone Loss ◽  
Bone Trabeculae

The effect of a new non-antimicrobial analog of tetracycline (CMT-8) on bone loss in ovariectomized (OVX) rats was examined. Three-month-old female rats were ovariectomized, and one week later, were distributed into 3 groups: sham-operated non-OVX controls, vehicle-treated OVX controls, and CMT-8-treated OVX rats. After 145 days of daily CMT-8 administration, the intact femurs were dissected and examined by several histological and histomorphometric techniques. OVX significantly (p < 0.01) decreased trabecular bone volume by 53.4% in the metaphyses compared with sham-operated controls. CMT-8 therapy produced a significant (p < 0.05) inhibition of trabecular bone loss and also induced bone formation in the OVX rats. Of interest, the newly synthesized bone in the CMT-treated OVX rats was found to increase the "connectivity" of the trabecular "struts" by bridging the adjacent longitudinal bone trabeculae, forming dense, platelike bone trabeculae. These results strongly suggest that long-term CMT-8 therapy effectively inhibits bone loss after OVX, not only by inhibiting bone resorption but also by inducing new bone formation in the trabecular areas of long bones.

High-impact exercise strengthens bone in osteopenic ovariectomized rats with the same outcome as Sham rats

2003 ◽  
Vol 95 (3) ◽  
pp. 1032-1037 ◽  
Author(s):  
Akiko Honda ◽  
Naota Sogo ◽  
Seigo Nagasawa ◽  
Takuya Shimizu ◽  
Yoshihisa Umemura
Keyword(s):  
Bone Mass ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Middle Aged ◽  
Mineral Density ◽  
Beneficial Effects ◽  
Ovx Rats ◽  
Jump Exercise ◽  
Body Weights ◽  
The Right

The effect of jump exercise on middle-aged osteopenic rats was investigated. Forty-two 9-mo-old female rats were either sham-operated (Sham) or ovariectomized (OVX). Three months after surgery, the rats were divided into the following groups: Sham sedentary, Sham exercised, OVX sedentary, and OVX exercised. Rats in the exercise groups jumped 10 times/day, 5 days/wk, for 8 wk, with a jumping height of 40 cm. Less than 1 min was required for the jump training. After the experiment, the right tibia and femur were dissected, and blood was obtained from each rat. OVX rats were observed to have increased body weights and decreased bone mass in their tibiae and femurs. Jump-exercised rats, on the other hand, had significantly increased tibial bone mass, strength, and cortical areas. The bone mass and strength of OVX exercised rats increased to approximately the same extent as Sham exercised rats, despite estrogen deficiency or osteopenia. Our data suggest that jump exercise has beneficial effects on lower limb bone mass, strength, bone mineral density, and morphometry in middle-aged osteopenic rats, as well as in Sham rats.

scholarly journals Red wine polyphenols modulate bone loss in the ovariectomized rat model of postmenopausal osteoporosis

2019 ◽  
Vol 70 (2) ◽  
pp. 1541
Author(s):  
C. PASSALI ◽  
A. PATSAKI ◽  
P. LELOVAS ◽  
N. ALIGIANNIS ◽  
M. MAKROPOULOU ◽  
...  
Keyword(s):  
Bone Loss ◽  
Rat Model ◽  
Red Wine ◽  
Bending Test ◽  
Ovariectomized Rats ◽  
Mineral Density ◽  
Ovx Rats ◽  
Trabecular Bone Loss ◽  
Wine Polyphenols ◽  
Red Wine Polyphenols

The aim of this study was to examine the effect of Red Wine Polyphenols (RWPs) extract on bone mineral density (BMD) in the ovariectomized (OVX) rat model. Thirty-five 10-month-old Wistar rats were separated into controls (CTRL), OVX and OVX plus RWPs in their drinking water (dose, 50 mg/kg body weight per day), starting immediately after OVX for 6 months. Βody and uterine weight, BMD of the tibia at baseline, 3 and 6 months post-OVX, and 3-pointing bending of the femur, were examined. Statistical comparison of the total tibia BMD within groups during the study period showed a significant reduction in the OVX and OVX+RWPs groups both from baseline to 3 and 6 months and from 3 to 6 months, whereas in the CTRL group, there was no significant change. For the proximal tibial metaphysis, comparison of BMD percentage changes from baseline to 3 months and 6 months and from 3 to 6 months revealed highly statistical differences between OVX and OVX+RWPs groups (P = 0.000). OVX induced a significant reduction of biomechanical parameters as expected; the 3-point bending test showed that the maximum force before fracture, energy absorption and fracture stress significantly increased in the OVX group treated with RWPs compared with the nontreated OVX rats (P = 0.048, P = 0.002 and P = 0.003, respectively). Dietary intake of RWPs for 6 months significantly prevented trabecular bone loss and improved bone strength in estrogen-deficient ovariectomized rats.

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