scholarly journals Parkia speciosaHassk.: A Potential Phytomedicine

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Yusof Kamisah ◽  
Faizah Othman ◽  
Hj Mohd Saad Qodriyah ◽  
Kamsiah Jaarin
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Carboxylic Acid ◽  
Southeast Asia ◽  
Plant Extract ◽  
Plant Extracts ◽  
Folk Medicine ◽  
Pharmacological Properties ◽  
Total Polyphenol ◽  
Ongoing Research

Parkia speciosaHassk., or stink bean, is a plant indigenous to Southeast Asia. It is consumed either raw or cooked. It has been used in folk medicine to treat diabetes, hypertension, and kidney problems. It contains minerals and vitamins. It displays many beneficial properties. Its extracts from the empty pods and seeds have a high content of total polyphenol, phytosterol, and flavonoids. It demonstrates a good antioxidant activity. Its hypoglycemic effect is reported to be attributable to the presence ofβ-sitosterol, stigmasterol, and stigmast-4-en-3-one. The cyclic polysulfide compounds exhibit antibacterial activity, while thiazolidine-4-carboxylic acid possesses anticancer property. The pharmacological properties of the plant extract are described in this review. With ongoing research conducted on the plant extracts,Parkia speciosahas a potential to be developed as a phytomedicine.

scholarly journals Isolation, Structure Elucidation, and Biological Activity of a New Alkaloid from Zanthoxylum rhetsa

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601
Author(s):  
Karsten Krohn ◽  
Stephan Cludius-Brandt ◽  
Barbara Schulz ◽  
Mambatta Sreelekha ◽  
Pottachola Mohamed Shafi
Keyword(s):  
Biological Activity ◽  
Carboxylic Acid ◽  
Medicinal Plant ◽  
Plant Extract ◽  
Structure Elucidation ◽  
Biologically Active ◽  
Reduction Product ◽  
Pharmacological Properties ◽  
Evergreen Tree

Several biologically active alkaloids (1-4, 6), including a new quinazoline-6-carboxylic acid (1), were isolated from the medicinal plant Zanthoxylum rhetsa, an evergreen tree, native to subtropical areas. Whereas the pharmacological properties of the plant extract and single constituents have been widely tested, we now show that all of the metabolites have antialgal activities, all but 6 are antibacterial, and 6 and the reduction product 5 (derived from 4) are also antifungal.

Antibacterial Activity of Some Plant Extracts Used in Folk Medicine

2008 ◽  
Vol 7 (3-4) ◽  
pp. 191-201 ◽  
Author(s):  
Rathish Nair ◽  
Tamanna Kalariya ◽  
Sumitra Chanda
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
Folk Medicine

scholarly journals Anticancer Activity, Antioxidant Activity, and Phenolic and Flavonoids Content of Wild Tragopogon porrifolius Plant Extracts

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Fuad Al-Rimawi ◽  
Suzi Rishmawi ◽  
Sharehan H. Ariqat ◽  
Mahmoud F. Khalid ◽  
Ismail Warad ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Plant Extracts ◽  
Folk Medicine ◽  
Reducing Power ◽  
Total Phenolic ◽  
Osteosarcoma Cell ◽  
Anticancer Activities ◽  
Antioxidant Power

Tragopogon porrifolius, commonly referred to as white salsify, is an edible herb used in folk medicine to treat cancer. Samples of Tragopogon porrifolius plant grown wild in Palestine were extracted with different solvents: water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (AA). Four different antioxidant assays were used to evaluate AA of the extracts: two measures the reducing power of the extracts (ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant power (CUPRAC)), while two other assays measure the scavenging ability of the extracts (2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid (ABTS)) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)). Anticancer activity of the plant extracts were also tested on HOS and KHOS osteosarcoma cell lines. The results revealed that the polarity of the extraction solvent affects the TPC, TFC, and AA. It was found that both TPC and AA are highest for plant extracted with 80% ethanol, followed by water, and finally with 100% ethanol. TFC however was the highest in the following order: 80% ethanol > 100% ethanol > water. The plant extracts showed anticancer activities against KHOS cancer cell lines; they reduced total cell count and induced cell death in a drastic manner.

scholarly journals Cyperus rotundus cyperaceae: a study of phytochemistry, total polyphenol content, flavonoid content, and antioxidant activity

2021 ◽  
Vol 332 ◽  
pp. 06003
Author(s):  
Quy Nguyen Ngoc ◽  
Tien Nguyen Minh
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Secondary Compounds ◽  
Cyperus Rotundus ◽  
Aqueous Extracts ◽  
Flavonoid Content ◽  
Total Polyphenol ◽  
Anti Cancer ◽  
Potential Applications ◽  
The Rich

Cyperus rotundus is a folk medicine with many benefits such as anti-inflammatory, anti-cancer, antioxidant, etc. It is widely used in many parts of the world. This study provided data on the phytochemical composition of Cyperus rotundus rhizome, including the active groups of alkaloids, flavonoids, phenols, tannins, and triterpenoids. The polyphenols content in the ethanolic and aqueous extracts of Cyperus rotundus were 26.89 ± 4.33 (mgGAE/g) and 51.84 ± 6.46 (mgGAE/g), respectively. While, the flavonoids content in the ethanolic and aqueous extracts of Cyperus rotundus were 78.03 ± 3.11 (mgQE/g) and 20.28 ± 2.25 (mgQE/g), respectively. According to the ABTS assay, the IC50 values of the aqueous and ethanolic extracts were 117.8 ± 19.85 (μg/ml) and 162.02 ± 11.43 (μg/ml), respectively. According to the DPPH assay, the IC50 values of Cyperus rotundus extracted by water and alcohol were 337.42 ± 22.84 (μg/ml) and 447.53 ± 33.8 (μg/ml), respectively. With the rich presence of secondary compounds, antioxidant activity confirmed in many studies, Cyperus rotundus is evaluated as having potential applications in many different fields such as pharmaceuticals, cosmetics, and functional foods.

scholarly journals Antimicrobial and Antioxidant Activities of Resins and Essential Oil From Pine (Pinus merkusii, Pinuso ocarpa, Pinus insularis) and Agathis (Agathis loranthifolia)

2017 ◽  
Vol 9 (1) ◽  
pp. 134
Author(s):  
Mardho Tillah ◽  
Irmanida Batubara ◽  
Rita Kartika Sari
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Positive Control ◽  
Diffusion Method ◽  
Pinus Merkusii

<p class="Abstract">The most common human pathogen that colonizes in a third of healthy people around the world are <em>Staphylococcus aureus</em>, and one of the materials allegedly able to overcome the pathogen is resin. Resin has been used in folk medicine for thousands of years to treat diseases. The antimicrobial activity of natural resins can be associated with a variety of organic compounds contained in them such as diterpenoids and triterpenoids. This research aimed to explore the antibacterial and antioxidant activities of <em>Pinus merkusii, P. oocarpa, P. insularis, Agathis loranthifolia</em> resins and essential oil. Resin was separated by distillation process to get essential oil and the residue was extracted using <em>n</em>-hexane, ethyl acetate (EtOAc), and methanol (MeOH). Antioxidant activity was performed by DPPH (1,1diphenyl-2-picryl hydrazyl) radical scavenging method. The antibacterial activity of resins and essential oil of the samples determined using the disc diffusion method against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. The results showed that the yield of resin extract was ranging from 8.44 % to 95.56%. All extracts and essential oil could not inhibit <em>E coli </em>growth but inhibit the <em>S. aureus </em>growth. This experiment concluded that resin <em>n</em>-hexane extract from <em>P.oocarpa</em> was the most potent as antibacterial activity against <em>S. aureus</em>. All of the samples used had less potential antioxidant activity compared to positive control ascorbic acid. Result of this study show that pine resin from Indonesia has potential as an antibacterial agent. </p>

scholarly journals Antibacterial and Antioxidant Activities of Centeurea damascena Methanolic Extract

2019 ◽  
Vol 5 (1) ◽  
pp. 55-63
Author(s):  
Mohammad Jaafreh ◽  
Khaled Khleifat ◽  
Haitham Qaralleh ◽  
Muhamad Al-limoun
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Plant Extracts ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Micrococcus Luteus ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
The Family

The family Asteraceae include large number of Centaurea species which have been applied in folk medicine. One of the family Asteraceae members is the Centaurea damascena which authentically been tested for its antibacterial and antioxidant activity as well as its toxicity. The aims of the study were to determine the antimicrobial and antioxidant activities and toxicity of methanolic plant extracts of Centaurea damascene. The methanolic extracts were screened for their antibacterial activity against nine bacteria (Staphylococcus aureus ATCC 43300, Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 10240, and Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 11293, Pseudomonas aerugino and Klebsiella pneumoniae, Enterobacter aerogenes ATCC 13048 and Salmonella typhi ATCC19430). The antibacterial activity was assessed by using the disc diffusion methods and the minimum inhibition concentrations (MIC) using microdilution method. The extracts from Centaurea damascene possessed antibacterial activity against several of the tested microorganisms. The MIC of methanol extract of C. damascene ranged from 60–1100 µg/mL. Free radical scavenging capacity of the C. damascena methanol extract was calculated by DPPH and FRAP test. DPPH radicals were scavenged with an IC50 value of 17.08 µg /ml. Antioxidant capacities obtained by the  FRAP was 51.9 and expressed in mg Trolox g-1 dry weight. The total phenolic compounds of the methanol extracts of aerial parts, as estimated by Folin–Ciocalteu reagent method, was about 460 mg GAE/ g. The phenolic contents in the extracts highly correlate with their antioxidant activity, (R2 = 0.976) confirming that the antioxidant activity of this plant extracts is considerably phenolic contents-dependent

scholarly journals In vitro investigation of antioxidant and antihemolytic activities of three Lamiaceae species from Morocco

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Abdelbassat Hmidani ◽  
Eimad dine Tariq Bouhlali ◽  
Mohammed Ajebli ◽  
Tarik Khouya ◽  
Mohamed Benlyas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protective Effect ◽  
Plant Extracts ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Ocimum Basilicum ◽  
Antioxidant Power ◽  
In Vitro Investigation ◽  
Origanum Majorana

Abstract Background Lkhzama (Lavandula officinalis), Mard-doch (Origanum majorana), and Lahbak (Ocimum basilicum) are aromatic and medicinal plants widely used in Moroccan folk medicine as a treatment for numerous diseases including liver diseases, rheumatism, and diabetes. This study was undertaken to examine the antioxidant and antihemolytic activities of the aqueous extracts of these plants. The antioxidant activity was evaluated using three in vitro tests: DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity, FRAP (ferric reducing antioxidant power assay), and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay. The antihemolytic activity of plant extracts was evaluated against AAPH (2,2′-azobis(2-amidino-propane) dihydrochloride)-induced erythrocyte hemolysis. Results Our findings showed that all plant extracts displayed significant antioxidant and antihemolytic effects. In fact, among the studied plant extracts, the highest antioxidant power was recorded in Origanum majorana, based on DPPH (IC50 = 12.29 μg/mL), ABTS (226.13 μmol TE/g DW), and FRAP (477.82 μmol TE/g DW) assays. Moreover, the same plant also showed the best membrane protective effect (269.55%). Whereas, Ocimum basilicum exhibited the lowest antioxidant activity using DPPH (IC50 = 42.85 μg/mL), ABTS (IC50 = 226.13 μmol TE/g DW), and FRAP (IC50 = 172.84 μmol TE/g DW) and, thus, the lowest membrane protective effect (182.70%). Conclusion This result supports the use of these plants in folk medicine for preventing and treating many diseases, especially those related to oxidative stress.

scholarly journals Phytochemical screening of extracts from Spiranthera odoratissima A. St.-Hil. (Rutaceae) leaves and their in vitro antioxidant and anti-Listeria monocytogenes activities

2020 ◽  
Vol 42 ◽  
pp. e51881
Author(s):  
Amanda de Oliveira Souza ◽  
Dâmaris Hadassa Rangel Fonseca Bessa ◽  
Cassia Cristina Fernandes ◽  
Paulo Sérgio Pereira ◽  
Carlos Henrique Gomes Martins ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Sesquiterpene Lactones ◽  
Phytochemical Screening ◽  
Antioxidant Power ◽  
Broth Microdilution Method

Spiranthera odoratissima A. St.-Hil (Rutaceae), a shrub whose common name is manacá do Cerrado in Brazilian Portuguese, is about 1-m high and has been used by folk medicine to treat stomachache, kidney and liver infections, headache, rheumatism and as a blood purifier. This study aimed at preparing hexane, ethyl acetate, methanolic, hydroethanolic and aqueous extracts from S. odoratissima leaves, at carrying out preliminary phytochemical screening and at evaluating their in vitro antioxidant and anti-Listeria monocytogenes activities. Antioxidant activity was evaluated by the DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azinobis-3-ethybenzothiazoline-6-sulfonate) and FRAP (ferric reducing antioxidant power) methods. Antibacterial activity was investigated against L. monocytogenes and Minimum Inhibitory Concentration (MIC) values of plant extracts were calculated by the broth microdilution method with the use of 96-well plates. In aqueous, methanolic, hydroethanolic, ethyl acetate and hexane extracts from S. odoratissima leaves, the following classes of compounds were investigated: organic acids, reducing sugars, flavonoids, saponin compounds, coumarin compounds, phenolics, tannins, purine compounds, catechins, flavonol derivatives, sesquiterpene lactones and anthraquinones. All plant extracts, except the hexane one, exhibited high antioxidant activity. Regarding antibacterial activity, the most polar extracts showed high activity against L. monocytogenes; their MIC values ranged between 12.5 and 62.5 µg mL-1, while the hexane one exhibited low activity (MIC = 1000 µg mL-1). In short, extracts from S. odoratissima leaves may be considered promising sources of secondary metabolites with relevant antioxidant and antibacterial activities.

scholarly journals Comparative Analysis of Phytochemical Constituents and Antibacterial Activity of Crude and Purified Ethanol and Ethyl-acetate Extracts of Euphorbia hirta L. Whole Plant

2019 ◽  
pp. 1-11
Author(s):  
A. T. Oseni ◽  
O. O. Olusola-Makinde ◽  
M. K. Oladunmoye
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Otitis Media ◽  
Plant Extract ◽  
Plant Extracts ◽  
Chemistry Laboratory ◽  
Euphorbia Hirta ◽  
Agar Diffusion ◽  
Whole Plant ◽  
Phytochemical Constituents

Aims: To study the phytochemical constituents and antibacterial efficacy of crude and purified Euphorbia hirta whole plant extract on Gram-positive bacteria isolated from otitis media. Study Design: Experimental Research design. Place and Duration of Study: Sample: Department of Microbiology (Mtech Laboratory) and Department of Chemistry (Organic Chemistry Laboratory), School of Sciences, Federal University of Technology, Akure, Ondo State, Nigeria. Between November 2018 and March 2019. Methods: The streak plate method was used for bacterial isolation, maceration method for Euphorbia hirta whole plant extraction using ethanol and ethyl-acetate as solvents. The E. hirta whole plant extracts were purified using column chromatography method. The extracts were assayed on the test bacterial isolates by agar diffusion technique. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts were carried out by agar dilution and agar diffusion techniques, respectively. Results: The ethanolic extract had the highest extraction yield (19%). The Staphylococcus aureus was resistant to multiple antibiotics: amoxicillin (30 µg), gentamycin (10 µg), and streptomycin (30 µg). The phytochemical screening of crude plant extracts showed presence of flavonoids, glycosides, saponins, tannins and terpenoids. At 100 mg/ml, crude and purified ethanol extracts showed antibacterial activity with 18±0.57 mm and 14±0.57 mm respectively on Streptococcus pyogenes. The MIC and MBC of purified ethanol extract ranged between 6.25-50 mg/ml and 25 mg/ml-100 mg/ml respectively. Conclusion: This research showed that E. hirta whole plant extract possesses antibacterial activity. The purified E. hirta whole plant extract showed higher inhibitory effect compared to crude extracts. This is an indication that purified E. hirta whole plant extract can be used in the development of novel therapeutic drugs in the treatment of otitis media.

scholarly journals To Develop the Method for UHPLC-HRMS to Determine the Antibacterial Potential of a Central American Medicinal Plant

Separations ◽  
2019 ◽  
Vol 6 (3) ◽  
pp. 37 ◽  
Author(s):  
Gaganpreet Kaur Monga ◽  
Anima Ghosal ◽  
Dil Ramanathan
Keyword(s):  
Antibacterial Activity ◽  
Plant Extract ◽  
Bacterial Growth ◽  
Plant Extracts ◽  
Mass Difference ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Liquid Chromatograph ◽  
Aechmea Magdalenae ◽  
Antibacterial Potential

The development of antibiotic resistance by microbials has long been acknowledged. The major challenge worldwide is to develop novel, natural, and potent antibiotics against the multidrug resistant bacteria. In this study, our aim was to develop the method for a highly sensitive instrument, ultra-high performance liquid chromatograph-high resolution mass spectrometer (UHPLC-HRMS), to evaluate the antibacterial property of a natural product. Aechmea magdalenae (Andre) Andre ex Baker, a plant belonging to the family Bromeliaceae, a native of Central America was used in this study. Based on the available literature, it was hypothesized that Aechmea magdalenae has antibacterial activity. In addition, the profiling done on A. magdalenae using gas chromatography-mass spectrometry (GC-MS) also revealed the presence of medicinally important chemical compounds, such as acetic acid. Minimum inhibitory concentration (MIC) of dried Aechmea plant extract was determined for the first time using 96-well plate assay, followed by determination of antibacterial potential using LC-MS. The reason being that other dried methanolic plant extracts, such as Vismia macrophylla, lined up for antibacterial testing have dark extracts, for which determining the antibacterial potential and reading the results with the naked eye would be challenging. To overcome the situation of dark plant extracts, a generalized novel LC-MS method was developed that was used for the plant A. magdalenae, and would be used further for other plants. A blue indicator called resazurin was added to the wells; resazurin, upon incubation with the living cells, got reduced to resorufin (which was pink), while it remained blue with bacterial growth inhibition. The mass difference created due to reduction of resazurin to resorufin was detected by using LTQ Orbitrap Discovery in positive ion mode to determine the antibacterial activity of the plant extract. The sample preparation for LC-MS assay included centrifugation of the samples taken from 96-well plate, followed by filtration of the supernatant, before exposing them to C-18 column. The results obtained from full scan LC-MS spectrum consistently demonstrated the presence of resorufin from wells with bacterial growth, and resazurin from wells with inhibition through peaks of relevant masses.

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