scholarly journals Antimicrobial Property of Extracts of Indian Lichen against Human Pathogenic Bacteria

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Priya Srivastava ◽  
D. K. Upreti ◽  
T. N. Dhole ◽  
Apurva K. Srivastava ◽  
Meghanand T. Nayak
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Streptococcus Faecalis ◽  
Human Pathogenic Bacteria ◽  
Usnea Ghattensis

Context.Usnea ghattensisG. Awasthi (Usneaceae) endemic fruticose lichen found growing luxuriantly in Northern Western Ghats of India, it also contains Usnic acid as a major chemical and tested against some human pathogenic bacteria.Objective. To explore antimicrobial properties ofUsnea ghattensisagainst some human pathogenic bacteria.Materials and Methods. The lichen was extracted in acetone, methanol, and ethanol.In vitroantimicrobial activity was tested initially byKirby-Bauertechnique of disc diffusion method and was confirmed by minimum inhibitory concentration using Broth microdilution method according to the NCCLS guidelines.Results. Ethanol extract was most effective againstBacillus cereusandPseudomonas aeruginosawith a zone of inhibition 29.8 ± 0.6 mm and 12.3 ± 0.5 mm diameters at a concentration of 0.2 mg/mL. Acetone and methanol extract demonstrated almost similar activity againstStaphylococcus aureusand the zone of inhibition was 24.6 ± 0.5 and 24.7 ± 0.4 mm. Only methanol extract was showing activity againstStreptococcus faecaliswith a 13.5 ± 0.8 mm zone. MIC value noted againstStaphylococcus aureusandStreptococcus faecaliswas 6.25 μg/mL and 25 μg/mL, whereas againstBacillus cereusandPseudomonas aeruginosa, MIC calculated was 3.125 μg/mL and 200 μg/mL, respectively.Conclusion. The present study demonstrates the relatively higher activity of this lichen against not only gram (+) but significantly also against gram (−) bacteria. This indicates that this lichen might be a rich source of effective antimicrobial agents.

scholarly journals Antimicrobial Efficacy of Mineral Trioxide Aggregate against Therapy Resistant Endodontic Microorganisms

2016 ◽  
Vol 33 (3) ◽  
pp. 140-145
Author(s):  
Mst Laila Akter Banu ◽  
AKM Bashar ◽  
Md Mujibur Rahman Howlader ◽  
Md Shamsul Alam ◽  
Md Ashraf Hussain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Zone Of Inhibition

Microorganisms, usually from the dental caries, are the main sources of diseases in dental pulp (root canals) and periapical region. Facultative bacteria and fungi have been identified in therapy resistant persistent endodontic infection. The objectives of this study was to evaluate the antimicrobial efficacy of Mineral Tri Oxide Aggregate (MTA) against therapy resistant endodontic microorganisms. The efficacy of MTA was also compared with that of calcium hydroxide. Six standard bacterial stains were used: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, bacillus subtilis, Candida albicans and Enterococcus faecalis. The agar diffusion method on Muller- Hilton media was employed. The plates containing media were inoculated with the specified bacterial suspensions. Two standard holes were prepared on each microorganism inoculated plate with a copper puncher and one hole was completely filled with MTA & the other with Ca (OH)2 . The plates were then kept at environmental temperature for one hour to ensure prediffusion and then incubated at 370C for 24 hours. After 24 hours, the diameters of inhibition zones were measured. Tests were replicated for thirty times for each sample and mean values were taken. Zone of inhibition as measured for MTA and Ca (OH)2 were statistically analyzed with Student’s t-Test and Post Hoc Games Howell Test and were presented as mean ± SD to compare of efficacy of MTA and calcium hydroxide on different microorganisms. Both MTA and Ca(OH)2 were found to produce zone of inhibition against Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), , Bacillus subtilis (BTCC 17 ), and Candida albicans (BTCC 493). MTA showed highest activity against S. aureus and lowest activity against P. aeruginosa which was similar to the activity range of Ca (OH)2 against the mentioned organisms. But both of them failed to produce any activity against E. coli and. E. faecalis. MTA was found to produce a lower efficacy than Ca (OH)2 while comparing the zone of inhibition between them and statistically it was significant. Mineral Tri Oxide Aggregate (MTA) showed antimicrobial efficacy against some therapy resistant microorganisms but it did not show antimicrobial efficacy against Escherichia coli and Enterococcus faecalis. MTA was found to produce a lower antimicrobial efficacy than Ca (OH)2.J Bangladesh Coll Phys Surg 2015; 33(3): 140-145

scholarly journals PENGARUH PEMANASAN, TINGKAT KEASAMAN (pH) DAN KONSENTRASI GARAM TERHADAP STABILITAS ANTI BAKTERI EKSTRAK ETANOL KULIT KAYU AKWAY (Drimys piperita Hook f.)

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba ◽  
Nitia Nilawati ◽  
Eka Syartika
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Heating Time ◽  
Diffusion Method ◽  
Ethanol Extracts ◽  
And Staphylococcus Aureus ◽  
Agar Well Diffusion Method

ABSTRACT  Akway (Drimys piperita) is a woody, evergreen and aromatic plant that belongs to family winteraceae. This plant is used by Sougb tribe lived in Sururey village, District of Anggi, to enhance the vitality of body. The objectives of the research were to determine antimicrobial stability of akway bark extracts influenced by heating time of 100OC, levels of acidity (pH) and salt contents.. Antimicrobial assays were done by using agar well diffusion method against four species of bacteria, i.e.  Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. The results showed that ethanolic extracts of akway bark only inhibited growth of B. cereus and S. aureus with minimum inhibitory concentration 0,99% and 0,89% . The levels of concentration and acidity of ethanol extracts  influenced the antimicrobial capacity of extracts.. Whereas heating time on 100OC during 25 minutes and salt contents  up to 5% of extract solution did not influence the antimicrobial stability of  akway bark extracts. Key words : akway, extracts, antimicrobe, pH, Heating, salt ABSTRAK Akway (Drimys piperita) adalah tumbuhan berkayu, aromatik dan hijau sepanjang tahun dan tergolong dalam suku winteraceae. Tumbuhan ini digunakan oleh Suku Sougb yang bermukim di desa Sururey Distrik Anggi, untuk mengobati malaria dan meningkatkan vitalitas tubuh. Tujuan dari penelitian ini adalah untuk menentukan stabilitas antimikroba ekstrak kulit kayu akway pada waktu pemanasan ekstrak pada 100OC, tingkat keasaman (pH) dan kandungan garam. Pengujian antimikroba dilakukan dengan menggunakan metode difusi agar terhadap empat spesies bakteri yaitu Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. Hasil menunjukkan bahwa ekstrak etanol kulit kayu akway hanya dapat menghambat bakteri Bacillus cereus dan Staphylococcus aureus dengan konsentrasi hambat minimum masing-masing adalah 0,99% dan 0,89%. Tingkat konsentrasi dan keasaman (pH) mempengaruhi kapasitas antimikroba ekstrak etanol kulit kayu akway. Sedangkan perlakuan pemanasan pada suhu 100OC dengan lama pemanasan sampai dengan 25 menit dan penambahan garam NaCl sampai konsentrasi 5%  tidak berpengaruh pada stabilitas antimikroba ekstrak etanol kulit kayu akway.  

scholarly journals Phytochemical and Antimicrobial Properties of Leaves ofAlchonea Cordifolia

2010 ◽  
Vol 7 (3) ◽  
pp. 1071-1079 ◽  
Author(s):  
N. J. George ◽  
I. B. Obot ◽  
A. N. Ikot ◽  
A. E. Akpan ◽  
N. O. Obi-Egbedi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Zone Of Inhibition ◽  
Bioactive Substances ◽  
Butanol Fraction ◽  
Zones Of Inhibition

The phytochemistry ofAchornea cordifolialeaf extract using different solvents was studied using standard methods. The effects of the leaf extract on some pathogenic bacteria and fungi were also examined. The Phytochemical screening of the leaves shows the presence of useful ethno-botanical bioactive substances such as tannin, saponin, flavonoid, cardiac glycoside and anthraquinone, while alkaloid, phlobatanin and terpene also tested for were absent. The butanol fraction of the extract gives the highest zone of inhibition (13.0 mm) againstStaphylococcus aureus, Escherichia coliandpseudomonas aeruginosa, which are in parity. The control, gentamycin injection gives 12.5 mm, 11.0 mm and 12.0 mm respective zones of inhibition against theStaphylococcus aureus, Eschariclia coliandPseudomonas aeruginosa. Also, the butanol fraction of the extract shows highest zone of inhibition of 17.0 mm againstCandida albican, while ethanolic extract gives 13.0 mm zone of inhibition againstTrichophyton violaceum.

scholarly journals Isolasi dan Karakterisasi Bakteri Endofit dari Tanaman Binahong dan Katepeng China

2014 ◽  
Vol 3 (2) ◽  
Author(s):  
Desriani Desriani ◽  
Ukhradia Maharaniq Safira ◽  
Maria Bintang ◽  
Akhmad Rivai ◽  
Puspita Lisdiyanti
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Clear Zone ◽  
Antibacterial Compounds ◽  
Cassia Alata ◽  
Potential Activity ◽  
Medical Effect

AbstrakBakteri endofit merupakan mikroorganisme menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman.Beberapa studi menunjukkan bahwa bakteri endofit tertentu dapat memproduksi senyawa kimia yang memiliki efek bagi kesehatan, terutama bakteri endofit yang diisolasi dari tanaman obat.Binahong (Anredera cordifolia) dan Ketepeng Cina (Cassia alata) merupakan tanaman obat yang telah diketahui memiliki beberapa khasiat.Tujuan dari penelitian ini ialah mengisolasi dan mengkarakterisasi bakteri endofit dari kedua tanaman tersebut melalui uji antibakteriterhadap tiga jenis bakteri patogen manusia (Pseudomonas aeruginosa, Staphylococcus aureus, dan Bacillus cereus). Metode: Jumlah bakteri endofit yang berhasil diisolasi dari dua tanaman tersebut berjumlah 73 isolat (37 isolat dari Binahong dan 36 isolat dari Ketepeng Cina). Hasil: Berdasarkan uji antibakteri terhadap tiga bakteri patogen diperoleh 16 isolat bakteri endofit yang memiliki aktivitas potensial (ditandai dengan terbentuknya zona hambat), yaitu 1 isolat dari Ketepeng Cina (terhadap Pseudomonas aeruginosa) dan 15 isolat dari Binahong. Pembahasan: Terbentuknya zona hambat mengindikasikan kemungkinan adanya senyawa kimia yang memiliki efek antibakteri.Kata kunci: bakteri endofit, Anredera cordifolia, Cassia alata, antibakteri.AbstractEndophytic bacteria is a beneficial microorganism that interacts with plant without causing any harm to the host. Most studies showed that certain endophytic bacteria can produce chemical compound which have medical effect, especially endophytic bacteria isolated from medicinal plant. Binahong (Anrederacordifolia) and KetepengCina (Cassia alata) are several of medicinal plants potential to be used for medical purpose. Methods: The objective of this study was to isolate and to characterize endophytic bacteria from both plants through antibacterial activity test against to three kinds of human pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus). Results: total isolated endophytic bacteria from both plants are 73 isolates (37 isolates from Binahong and 36 isolates from KetepengCina). There were 16 isolates have potential activity (indicated by clear zone), one isolate from KetepengCina and 15 isolates from Binahong. Discussion: The formed clear zone indicates antibacterial compounds produced by endophyticbacteriatested. Further research is needed to identify the type of antibacterial compounds produced.Keywords:endophytic bacteria, Anrederacordifolia, Cassia alata, antibacterial.

scholarly journals SYNTHESIS AND ANTIMICROBIAL PROFILE OF SOME NEWER HETEROCYCLES BEARING THIAZOLE MOIETY

2012 ◽  
Vol 20 (20) ◽  
pp. 61-67
Author(s):  
RAJUL GUPTA ◽  
Neeraj Kumar FULORIA ◽  
Shivkanya FULORIA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Acetic Anhydride ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method ◽  
Antibacterial And Antifungal

Various substituted acetophenones on treatment. with iodine and thiourea yielded 2-amino-4-( substituted-phenyl)-thiazole, which on further treatment with acetic anhydride generated N-(4-(substituted phenyl)thiazol-2-ylacetamide(1-5). All the synthesized compounds were characterized by their respective FTIR, 1H NMR, and mass data. Synthesized compounds (l, 2, 3, 4, 5) when subjected to investigation for their antimicrobial activities i.e. antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus flavus, and Aspergillus fumigatus by disk diffusion method, revealed that compound 2 deemed to be most potent with the largest zone of inhibition.

scholarly journals Antibacterial and Antifungal Activity Analysis of Essential Oil of Pogostemon cablin (Blanco) Benth

2016 ◽  
Vol 30 (1-2) ◽  
pp. 7-10 ◽  
Author(s):  
Puspa Das ◽  
Shoma Dutta ◽  
Jaripa Begum ◽  
Md Nural Anwar
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Pogostemon Cablin ◽  
Antibacterial And Antifungal

The essential oil of Pogostemon cablin (Blanco) Benth, also known as Patchouli oil was subjected for its antimicrobial investigation against a panel of ten human pathogenic bacteria and six human pathogenic fungi by Agar well diffusion method and Macrobroth dilution technique using Ampicillin (20ìg/well) and Nystatin (20ìg/well) as control. Antibacterial activity revealed that, the essential oil was more active against Gram positive bacteria than Gram negative bacteria. The largest zone of inhibition was 35 mm (against Bacillus cereus) with 20 ìl of oil. Ditermination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) showed that, Bacillus cereus exhibited the lowest MIC (250 ìg/ml) and MBC (750 ìg/ml). The oil showed moderate antifungal activity against all tested organisms. Candida albicans showed greater zone of inhibition (16 mm) than Saccharomyces cerevisiae (14 mm) with 20 ìl and Candida albicans showed lowest MIC and MFC (both were 750 ìg/ml). The zone of inhibition was 25 mm for each filamentous fungal strain with 20 ìl, except for Rhizopus oligosporus (15 mm) and the lowest MIC (250 ìg/ml) and MFC (500 ìg/ml) were reported for Aspergillus fumigatus.Bangladesh J Microbiol, Volume 30, Number 1-2,June-Dec 2013, pp 7-10

scholarly journals Effect of AZADIRACHTA INDICA (Neem) leave extract on Some Selected Bacteria

2021 ◽  
Vol 3 (Number 2) ◽  
pp. 3-6
Author(s):  
Tuhin Jahan ◽  
Zinnat Ara Begum ◽  
Sayeeda Sultana ◽  
Rokhsana Dil Afroz ◽  
Mosa Shaheli Binty Hossain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Azadirachta Indica ◽  
Average Diameter ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Dilution Method ◽  
Zone Of Inhibition ◽  
Agar Disc

Azadirachta indica (Neem) is an herbal plant widely distributed in our subcontinent during all seasons. This study was carried out to detect the effect of Azadirachta indica (Neem) leave extract on some selected bacteria. The prospective study was carried out during the period of July 2005 to June 2006. Micro-organisms which are commonly responsible for infection in our country such as Staphylococcus aureus, Salmonella typhi, Escherichia coli and Pseudomonas aeruginosa were considered. Extract of Neem leave that is Aqueous Neem leave extract (ANLE) was prepared by filtration and evaporation. Effect against bacteria was examined by detection of minimum inhibitory concentration (MIC) by using ‘Broth dilution method’ and by detection of bacterial susceptibility by ‘Agar disc diffusion method’ and compare with cefepime. For ANLE the MICs against Staphylococcus aureus, Salmonella typhi, Escherichia Coli and Pseudomonas aeruginosa was 800 μg/ml, 1200 μg/ml, 1400 μg/ml and 1400 μg/ml respectively. With those MICs the average diameter of zone of inhibition against staphylococcus aureus with ANLE and cefepime was 20mm and 30mm respectively. Salmonella typhi exhibit sensitivity with zone of inhibition of 19mm, 26.5mm against ANLE and cefepime. Escherichia coli were sensitive against ANLE and cefepime with 17.5mm, 23.5mm zone of inhibition respectively. For Pseudomonas aeruginosa zone of inhibition was 16mm, and 21mm by ANLE, and cefepime respectively. Aqueous Leave extract of Azadirachta indica (Neem) have antibacterial activity against ‘staphylococcus aureus, salmonella typhi, Escherichia coli and Pseudomonas aeruginosa’ compared with Cefepime. Among all test bacteria staphylococcus aureus has lowest MICs.

scholarly journals Preliminary Phytochemical and Pharmacological Investigations of Alpinia conchigera Griff. and Plumbago indica L.

2012 ◽  
Vol 15 (2) ◽  
pp. 153-157 ◽  
Author(s):  
Mohammed Ibrahim ◽  
Joy Baura ◽  
Torequl Islam ◽  
Zilly Homa ◽  
M Mohi Uddin Chowdhury ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Pharmaceutical Journal ◽  
Salmonella Typhi ◽  
Shigella Dysenteriae ◽  
Blastomyces Dermatitidis ◽  
Zone Of Inhibition ◽  
Antibacterial And Antifungal Activities ◽  
Plumbago Indica

Preliminary phytochemical screenings with the crude extractives of Alpinia conchigera Griff. and Plumbago indica L. demonstrated the presence of alkaloids, steroids, saponins and reducing sugars. The antibacterial and antifungal activities of methanol extracts of A. conchigera and P. indica have been evaluated against 4 Gram positive and 7 Gram negative pathogenic bacteria and 7 fungi using ciprofloxacin and fluconazole as standards, respectively, where the extract showed varying degrees of antimicrobial activity with zone of inhibition ranging from 15.0 to 27.0 mm. A. conchigera demonstrated significant zone of inhibition against Aspergillus niger, Blastomyces dermatitidis, Staphylococcus aureus, Salmonella typhi, Shigella dysenteriae, Bacillus cereus Pseudomonas aeruginosa, Shigella sonnei, Vibrio cholerae while P. indica revealed strong inhibitory activity against Candida albicans, Blastomyces dermatitidis, Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella paratyphi. The MICs of the both plant extractives were found to be 31.25 ?g/ml against B. cereus, S. paratyphi, V. cholerae, A. niger and B. dermatitidis. In the brine shrimp lethality bioassay, the LC50 and LC90 of A. conchigera and P. indica were found to be 6.1 & 12.2 ?g/ml and 5.0 &12.0 ?g/ml, respectively. A significant dose dependent antidiarrhoeal, antimotility and analgesic activities were observed during screening in mice. DOI: http://dx.doi.org/10.3329/bpj.v15i2.12581 Bangladesh Pharmaceutical Journal 15(2): 153-157, 2012

scholarly journals Aktivitas Antibakteri Ekstrak Etilasetat Kulit Kayu Akway (Drimys piperita Hook f.) Pada Bakso Daging Sapi Selama Penyimpanan

2020 ◽  
Vol 9 (2) ◽  
pp. 50-56 ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba ◽  
Nitia Nilawati ◽  
Eka Syartika
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Cold Storage ◽  
Room Temperature ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Growth Of Bacteria

Akway (Drimys piperita Hook.f.) adalah tumbuhan obat yang digunakan oleh Suku Sougb Kabupaten Pegunungan Arfak Papua Barat untuk mengobati malaria. Penelitian-penelitian yang telah dilakukan menunjukkan bahwa tumbuhan obat bersifat antibakteri yang kuat. Penelitian ini bertujuan untuk mengevaluasi potensi antibakteri ekstrak etilasetat kulit kayu akway secara in vitro dan aplikasinya sebagai pengawet bakso daging sapi dalam penyimpanan suhu ruang dan refrigerator. Pengujian aktivitas antibakteri ekstrak secara in vitro dilakukan pada konsentrasi 0-25% (b/v) dengan menggunakan metode difusi sumur dalam medium agar sedangkan pengujian aktivitas antibakteri dalam model pangan bakso daging sapi dilakukan pada konsentrasi 0-0,75% (b/v) dengan menggunakan metode angka lempeng total. Hasil menunjukkan bahwa ekstrak etilasetat dapat menghambat pertumbuhan Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 dan Staphylococcus aureus ATCC25923 secara in vitro dengan konsentrasi penghambatan minimum sebesar 0,27-0,77% (b/v). Konsentrasi ekstrak berpengaruh nyata terhadap diameter zona hambat pertumbuhan bakteri. Semakin tinggi konsentrasi ekstrak yang digunakan menyebabkan semakin besar pula zona hambat pertumbuhannya. Pengujian aktivitas antibakteri ekstrak terhadap pertumbuhan bakteri dalam bakso daging sapi menunjukkan bahwa pencelupan bakso dalam ekstrak dengan konsentrasi 0,25-0,75% (b/v) dapat menghambat pertumbuhan bakteri selama 3-8 hari untuk penyimpanan pada suhu ruang sedangkan pada penyimpanan refrigerator adalah 15-33 hari. Kesimpulannya, ekstrak etilasetat kulit kayu akway sangat berpotensi digunakan sebagai pengawet model pangan bakso daging sapi.Antibacterial Activity of Ethyl Acetate Extracts of Akway (Drimys piperita Hook.f.) Barks on Meatballs during Storage AbstractAkway (Drimys piperita Hook.f.) is a medical plant used by Sougb Tribe, Arfak Mountains Regency, West Papua Province to heal malaria. Previous researches indicated that medical plants had strong antibacterial properties. The objectives of the research were to evaluate in vitro antibacterial potency of ethylacetate extracts of akway barks and its applications as preservative agent on meatballs during room temperature and cold storage. In vitro antibacterial assays were done on concentration of 0-25% (w/v) using agar well diffusion method while antibacterial assays on meatballs on concentration of 0-0.75% (w/v) were performed using total plate counts methods. Results showed that ethylacetate extracts inhibited in vitro growth of Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923 with MIC of 0.27-0.77% (w/v). Concentration of extracts significantly affected the growth of bacteria. The increasing of extract concentrations result in increasing diameters of growth inhibition zones. Meatballs soaked in extract solutions with concentrations of 0.25-0.75% (w/v) inhibited growth of bacteria during 3-8 days in room temperature storage and 15-33 days during cold storage in refrigerator. As conclusion, ethylacetate extracts of akway barks had high potent use as preservative agent in food model of meatballs.

scholarly journals Antimicrobial Activity of Benzimidazoles Derivatives

2020 ◽  
Vol 4 (11) ◽  
pp. 23-27
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Diffusion Method ◽  
Pathogenic Microorganisms ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Electron Withdrawal ◽  
Microbial Action

The anti-microbial action of benzimidazole of ampicillin, was examined in Vitro beneath a specific conditions, utilizing the disc diffusion method, against different gram-positive and gram-negative pathogenic microorganisms such as Bacillus cereus, Staphylococcus aureus Pseudomonas aeruginosa and Escherichia Coli. An arrangement of these compounds were arranged and have appeared to hinder phthogenic development, the range of the zone of restraint. The zone of Obstacles of compounds founds from 7 mm2 to 46 mm2 Among the synthesized compounds in common. 6-Methoxy-N-phenyl- 1H-benzo [d]imidazol-2-amine(1c, zone of inhibition 9 mm2 at 40 µg/ml against Escherichia coli ) and 6-Chloro-N-phenyl-1H-benzo [d]imidazol-2-amine (1d, zone of inhibition 18 mm2 at 40 µg/ml against Bacillus cereus) have appeared great movement. While 1j, zone of inhibition 20 mm2 at 40 µg/ml), and (1i, zone of inhibition 20 mm2 at 40 µg/ml) were found to be displayed directly to great action against Bacillus cereus and Escherichia coli. 1p did not appear any movement against Staphylococcus aureus .1e did not appear any movement against Escherichia Coli and Staphylococcus aureus.The results have shown clearly that the contribution of el ectron -donation and electron- withdrawal to the aromatic ring i ncreases antibacterial activity. Target benzimidazolessamples sho wed antibacterial & reference antibiotic ampicillin in vitro.

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