Serum Prolactin Levels in Psoriasis Vulgaris

Background. Psoriasis is a chronic inflammatory skin disease affecting approximately 1–3% of Caucasians. Prolactin has proliferative effects on human keratinocytes, a dominant feature of psoriasis, and it is thought that this hormone may play a role in the pathogenesis of the disease. This study was conducted to confirm or refute these findings in order to better understand the disease pathogenesis. Methods. The subjects were 90 individuals aged between 15 and 47 years. They were divided into three groups of 30 individuals each: psoriatic patients, atopic dermatitis patients, and control group. A questionnaire was filled regarding their demographic and medical history. All of the study subjects underwent venous blood sampling (5 mL), and serum TSH and prolactin levels were checked. Subjects with abnormal TSH were omitted. Results. None of the patients in the study had raised prolactin, and there was no significant difference in the serum prolactin level between patients with psoriasis and atopic dermatitis and the control group. There was no relationship between the severity of psoriasis and serum levels of prolactin. Conclusion. Prolactin does not seem to play a role in the pathogenesis of psoriasis as its serum levels are comparable with atopic dermatitis patients and that of the normal population.

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Studying the Frequency of Autoimmune Thyroid Diseases in Duhok Province

Science Journal of University of Zakho ◽

10.25271/sjuoz.2019.7.2.587 ◽

2019 ◽

Vol 7 (2) ◽

Author(s):

Rozheen I. Hasan ◽

Amir H. Raziq

Keyword(s):

Venous Blood ◽

Serum Levels ◽

Control Group ◽

Thyroid Disorder ◽

Control Groups ◽

Autoimmune Thyroid Diseases ◽

Autoimmune Thyroid ◽

Significant Difference ◽

The One ◽

And Control

Thyroid disorders are one of the most frequent disorders affecting endocrine system. The present study aimed to identify the frequency of thyroid disorder in our locality at Duhok city. To do so, a total of one hundred patients and one hundred apparently healthy control individuals were enrolled in this study. Samples of venous blood (5 ml) were withdrawn from patients and control subjects and analysed. Clinical assessment was performed by a specialist and each patient provided with a document supporting preliminary clinical diagnosis. Measurements of serum TSH, T3, T4, and anti-thyroperoxidase antibodies (anti-TPO antibodies) serum levels were performed. All assays were conducted at Duhok central health laboratory. Out of the one hundred patients and the one hundred control groups, forty-two and thirty candidates were randomly selected, respectively. The mean TSH serum levels of 25.87 (uIU/ml) for the patients significantly differ from that of the control group 3.55 (uIU/ml) (p<0.001). However, T3 and T4 serum levels indicated no significant difference between the two categories. Moreover, there was statisticaly significant difference (p< 0.0001) between the patients and control groups when their mean serum anti-TPO concentrations were compared. In conclusion, considerable number of subjects appeared to be candidates for deranged thyroid functions and is susceptible to autoimmune thyroid disorder.

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Plasma kisspeptin and ghrelin levels in puberty variant cases

Journal of Pediatric Endocrinology and Metabolism ◽

10.1515/jpem-2016-0127 ◽

2017 ◽

Vol 30 (5) ◽

Cited By ~ 2

Author(s):

Erdal Kurnaz ◽

Yaşar Şen ◽

Süleyman Aydın

Keyword(s):

Serum Levels ◽

Control Group ◽

Control Groups ◽

Premature Thelarche ◽

Premature Adrenarche ◽

Significant Difference ◽

And Gender ◽

And Control ◽

Relationship Of ◽

The Relationship

AbstractBackground:The aim of this study was to determine the serum levels of kisspeptin and ghrelin (GAH), as well as the relationship of these two peptides with each other in premature thelarche (PT) and premature adrenarche (PA) cases and to investigate the possibility of using these peptides as markers in the differentiation of puberty disorders.Methods:A PT group aged 1–8 years (n = 40), a PA group aged 1–9 years (n = 23, female/male = 20/3) and control groups consistent with each of the previous groups in terms of age and gender were created for the study. Kisspeptin and ghrelin levels were measured with ELISA methods from blood samples drawn while fasting in the morning.Results:When the PT group was compared with the controls, the plasma kisspeptin levels of the cases were significantly higher than the control group (165.47 ± 15.45 pmol/L, 96.82 ± 12.33 pmol/L, p = 0.005, respectively). Kisspeptin levels in the PA group did not show a difference with the control group (121.36 ± 17.99 pmol/L, 95.52 ± 11.54 pmol/L, p = 0.249, respectively). No significant difference could be found when GAH levels in the PT and PA groups were compared with controls. No significant correlation was found between kisspeptin and GAH levels in the PT and PA groups.Conclusions:Our results indicate that kisspeptin plays an important role in the PT, but GAH is not associated with puberty disorders.

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The Effect of Balloon Inflating Distraction on Pain Intensity During Venous Blood Sampling in Children: A Randomized Controlled Trial

10.21203/rs.3.rs-711305/v1 ◽

2021 ◽

Author(s):

Somaeh. Salehi Sarbijan ◽

Roghiah Mehdipoor Rabori ◽

Esmat Nouhi

Keyword(s):

Venous Blood ◽

Blood Sampling ◽

Control Group ◽

Negative Consequences ◽

Randomized Controlled ◽

Pain Scores ◽

Significant Difference ◽

Average Pain ◽

And Control ◽

Venous Blood Sampling

Abstract Background: A large number of children are subject to prevention, diagnosis, and treatment procedures that mostly require venous blood sampling. Painful procedures such as blood sampling in children have annoying physical and emotional effects and can lead to wider negative consequences such as physical, behavioral, and social disorders in children. This study aimed to determine the effect of distraction by balloon inflating on the amount of pain during taking blood samples from children aged 4 to 7. Method: This study had a randomized controlled clinical trial that was conducted on 80 children admitted to the pediatric department of Imam Khomeini Hospital in Jiroft-Kerman province who met the inclusion criteria after obtaining their parent’s consent. The children were randomly grouped into the intervention (n = 40) and control (n = 40) groups. The pain level in children in the intervention group was measured during the venous blood sampling while the children were watching inflating a balloon. However, the pain in children in the control group was measured without any intervention during blood sampling. Wong–Baker Faces Pain Rating Scale questionnaire was used for pain assessment. Results: The average pain scores for the children in the two groups showed no significant difference before the intervention but the average pain scores after the intervention in the intervention and control groups were 1.38±2.56 and 4.2±1.58; showing a significant difference between the two groups after the intervention (p-value < 0.05). Conclusion: Using balloon inflation distraction during venous blood sampling reduces pain in children.

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Associations of SNP rs_7927894 of FLG gene and TARC/CCL17 with atopic dermatitis in children

Modern pediatrics. Ukraine ◽

10.15574/sp.2021.118.12 ◽

2021 ◽

pp. 12-18

Author(s):

V.O. Dityatkovsky ◽

◽

O.E. Abaturov ◽

N.V. Naumenko ◽

O.O. Alifirenko ◽

...

Keyword(s):

Atopic Dermatitis ◽

Statistical Significance ◽

Venous Blood ◽

Clinical Signs ◽

Main Group ◽

Control Group ◽

Serum Concentrations ◽

Control Groups ◽

And Control ◽

Severity Degree

One of the main genetic factors of the development of atopic dermatitis (AD) in children are single nucleotide polymorphisms (SNP) of the filagrin gene (FLG), particularly rs_7927894 FLG. One of the mostly studied and promising AD marker chemokines (CK) is the thymusE and activation regulated chemokine (TARC/CCL17). Purpose – to detect the associations and role of different variants of SNP rs_7927894 FLG gene and TARC/CCL17 in children suffering different AD clinical proE files (CP) – isolated or combined with comorbid atopic disorders (AtD). Materials and methods. The main group comprised 39 patients aged 3 to 18 years, suffering the isolated AD or combined with comorbid AtD. The control group comprised 47 patients aged 3 to 18 years, suffering the pathology of gastrointestinal tract without clinical signs of atopy. All the patients of the main and control groups had undergone detection of the genotype variants of SNP rs_7927894 FLG gene by real-time polymerase chain reaction and detection of TARC/CCL17 serum concentrations in venous blood. The cutEoff value of statistical significance was set as p<0.05. Results. The incidence and association of genotype variants C/C, C/T and T/T SNP rs_7927894 FLG gene in patients of cohorts of the studied groups were detected as follows: C/T rs_7927894 FLG was significantly the most common in the general main group (56.4%, p<0.05), within the cohort of CP AD isolated (61.1%, p<0.05) and CP of AD combined with comorbid AtD (52.4%, p<0.05). There were detected the associations of studied SNP with AD: C/T rs_7927894 FLG is significantly directly associated with AD (r=0.291, p<0.05), C/C rs_7927894 FLG has a reverse association with a trend to significance (r=-0.194, p=0.07). Mean serum concentrations of TARC/CCL17 did not differ significantly among patients cohorts of the main and control groups, respectively: general main group — 615.8 pg/ml, main with a CP AD isolated — 651.3 pg/ml, main with a CP of AD combined with comorbid AtD — 585.4 pg/ml, control — 608.4 pg/ml (p>0.05). Associations of serum TARC/CCL17 concentrations were determined as follows: elevation trending to significance within increasing AD severity degree (r=0.290, p=0.07) and significant elevation within the AD exhacerbation period (r=0.426, p<0.05). No significant association of TARC/CCL17 as to AD patients compared to the control group was detected in our study (r=-0.027, p>0.05). Conclusions. The genotype heterozygote variant C/T rs_7927894 FLG is significantly the most common and associated with all AD CP in children — isolated and combined with comorbid AtD. Variant C/C rs_7927894 of FLG gene is significantly reversely associated with AD in children. Serum concentrations of TARC/CCL17 did not reveal any significant differences between the AD patients and nonEatopic ones. However, they significantly elevate within AD exacerbation phase and trending to significance within AD severity degree increase in children. The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local Ethics Committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the authors. Key words: atopic dermatitis, children, associations, polymorphism, filaggrin, thymus- and activation regulated chemokine.

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Serum changes of PYD, C2C and OC among male brucellosis patients at early period

10.21203/rs.3.rs-28072/v1 ◽

2020 ◽

Author(s):

Qiang Li ◽

Lansheng Hu ◽

Zhijun Zhao ◽

Li Ma ◽

Jiquan Li ◽

...

Keyword(s):

Early Stage ◽

Venous Blood ◽

Early Period ◽

Serum Levels ◽

Control Group ◽

Human Brucellosis ◽

Male Patients ◽

And Control ◽

Control Study ◽

Elisa Data

Abstract Objective Musculoskeletal changes are the most common clinic manifestation of brucellosis,and these musculoskeletal changes are irreversible, so, it is very important to prevention musculoskeletal changes at early stage of human brucellosis . Method This was a case-control study. According to diagnostic criteria of human brucellosis in China(WS269-2007),41 male patients were diagnosed as brucellosis patients at early period (within 6 months) and they were not therapied with drug, 44 \ persons were divided into control group with randomly matching. Venous blood samples were collected from all study subjects, and serum PYD ༌C2C and OC were quantitative measured with method of ELISA. Data were analyzed using SPSS 17.0 software. A p value < 0.05 was considered to be statistically significance. Result The median levels of serum PYD, C2C and OC in the patients group were 278.53 ug/l, 82.23 ug/l, and 8.41ug/l, respectively, while the median levels of serum PYD, C2C and OC in the control group were 210.54 ug/l, 72.74 ug/l and 7.43 ug/l, respectively, there were existing significant differences between patients group and control group (Z = 5.686, 3.997, 3.579, all P = 0.000). Conclusion Serum levels of PYD, C2C and OC were increased among male brucellosis patients at early period, which might be the indicator biomarkers for osteoarticular changes of human brucellosis at early stage..

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Association among Thyroid Dysfunction, Asthma, Allergic Rhinitis and Eczema in Children with Alopecia Areata

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2017.050 ◽

2017 ◽

Vol 5 (3) ◽

pp. 305-309 ◽

Cited By ~ 1

Author(s):

Javad Ghaffari ◽

Ghasem Rahmatpour Rokni ◽

Armaghan Kazeminejad ◽

Hosein Abedi

Keyword(s):

Allergic Rhinitis ◽

Atopic Dermatitis ◽

Alopecia Areata ◽

Allergic Diseases ◽

Control Group ◽

Case Group ◽

Control Groups ◽

Significant Difference ◽

History Of ◽

And Control

BACKGROUND: Alopecia areata is a non-scarring hair loss, which typically starts quickly. Atopy is one of the possible predisposing risk factors for this condition.AIM: This study aimed to evaluate the prevalence of thyroid disease, atopic dermatitis and allergic diseases in children with alopecia areata and compare the results with healthy individuals.METHODS: This case-control study was conducted on 50 patients with alopecia areata, diagnosed by a dermatologist, and 150 healthy individuals as the control group. Participants filled the questionnaires, and necessary tests were performed.RESULTS: In this study, the mean age of the participants was 2.55 Â± 14.26 and 3.19 Â± 11.92 in the case and control groups, respectively. Prevalence of asthma was 22% in the case group and 12.5% in control group (P = 0.109). Also, allergic rhinitis and eczema were observed in 20% and 22% of the subjects of the case group, whereas they were reported to be 8% and 10% in the control group (PV = 0.03 and 0.175, respectively). Moreover, 28% and 8% of the participants in the case and control groups had a family history of atopy and allergic disorders, respectively (P = 0.046). A significant difference was observed between the two groups regarding gender, type of delivery and contact with animals.CONCLUSIONS: According to the results of this study, a significant association was observed between the prevalence of alopecia areata and atopic conditions, such as allergic rhinitis and history of atopic dermatitis.

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Serum Interleukin-15 in Alopecia Areata and its correlation with dermoscopic findings

QJM ◽

10.1093/qjmed/hcab093.011 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Naziha Hafez Khafagy ◽

Marwa Salah El Din Zaki ◽

Aya Mahmoud Hussein

Keyword(s):

Hair Loss ◽

Alopecia Areata ◽

Serum Levels ◽

Common Finding ◽

Control Group ◽

University Hospitals ◽

Exclamation Mark ◽

Significant Difference ◽

Initial Onset ◽

And Control

Abstract Background Alopecia areata is an autoimmune hair loss which frequently starts in childhood. Its presentation had an extreme variability not only in the time of initial onset but also in the duration, extent, and pattern of hair loss during any given episode of active loss. Moreover, the course of disease is unpredictable, with spontaneous regrowth of hair occurring in 80% of patients within the first year and sudden relapse at any given time. Due to the clinical variability and unpredictable nature of spontaneous regrowth, diagnosis and management may be difficult and challenging. Objective The aim of this study is to evaluate the serum levels of IL-15 in active alopecia areata and correlate them with disease severity and activity according to dermoscopic findings. Methods This case-control study were conducted in Dermatology, Venereology and Andrology department, Ain Shams University Hospitals included 30 patients with different clinical variants of AA, the diagnosis was made via clinical examination and dermoscopic findings. In addition, 30 apparently healthy individuals of matched age and sex as a control group were included in the study. Results Dermoscopic examination among cases showed that the most common dermoscopic findings in patients were vellus hair and yellow dots, while the least common finding was exclamation mark hairs. On comparing serum IL-15 in patients and control groups, it was found that serum levels of IL-15 in patients were significantly higher than those in the control group. There was no statistically significant difference in serum IL-15 levels between patients with negative and positive pull test, nail involvement, or body involvement. Similarly, no statistically significant difference in serum IL-15 levels in patients with various subjective disease activity was detected. However, there was a highly significant difference between serum IL-15 levels in different SALT score groups, with the highest levels being in the S3 group. There was a highly significant difference between IL-15 levels in patients with and without black dots. Also, there was significant difference between IL-15 in patients with and without broken hair, and exclamation mark hair. There was no significant difference in level of IL-15 among patients with and without yellow dots, and with and without vellus hair. Conclusion On the basis of the current study, we can conclude that IL-15 is significantly elevated in AA patients when compared to the control subjects. It is also a possible marker of AA severity. It is positively correlated with dermoscopic findings in AA patients, so dermoscopic findings can be useful in evaluating severity of alopecia areata.

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The comparison of total capacity antioxidant in the serum of people with pterygium and control subjects

International Eye Research ◽

10.18240/ier.2020.01.02 ◽

2020 ◽

Vol 1 (1) ◽

pp. 7-12

Author(s):

Heydari Behrooz ◽

◽

Zarban Asghar ◽

Hosseini Rad Abbas ◽

Feizmohammadi Akram ◽

...

Keyword(s):

Antioxidant Capacity ◽

Clinical Symptoms ◽

Venous Blood ◽

Power Measurement ◽

Control Group ◽

Antioxidant Power ◽

Control Subjects ◽

Significant Difference ◽

The Mean ◽

And Control

AIM: To investigate the comparison of total antioxidant capacity in the serum of patients with pterygium and control subjects. METHODS: This case-control study was conducted on all persons referred to Ophthalmology Clinic of teaching Hospital of Vali-Asr (peace upon to him) with clinical symptoms of pterygium during the year 2016. The control group was selected among patients referred to the Ophthalmology Clinic of Vali-Asr (peace without pterygium) that the two groups were matched in terms of age, gender and place of residence. Sixty-six persons [31 people (47%) in patient group and 35 people (53%) in the control group] were enrolled by convenience sampling. Venous blood sample was taken from all patients after the sampling using ferric reducing antioxidant power (FRAP); FRAP- as a quick 10min measurement, the antioxidant power measurement of samples according to the conversion of ferric iron (Fe3+) to ferrous iron (Fe2+) was checked. The collected data ware entered to software SPSS 21 and were analyzed by chi-square and Mann-Whitney tests at the level of α =0.05. RESULTS: The mean of antioxidant capacity in patients was 842/55±161/46 μmol/L and antioxidant capacity in healthy controls was 856/77±209/41 μmol/L (P=0.8). In the comparison of mean serum antioxidant capacity in healthy individuals and in the serum of people with pterygium based on gender the results showed that the antioxidant capacity mean in male control subjects has been 894/05± 176/82 μmol/L and in females control 780/01±118/33 μmol/L that the observed difference have been reported statistically significant (P=0.008) but the other comparison according the gender between cases and control does not show any significant difference. CONCLUSION: The results of this study showed that the full level of serum antioxidant capacity in patients has been less than the mean of antioxidant capacity in control subjects; however, the observed difference has not been significant. The results of this study were consistent with basic results carried out on the damaging effects of oxidative stress in the pterygium pathogenesis. Recommending diet with minerals and vitamins containing antioxidants may be preventing the onset and progression of pterygium.

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Genetic polymorphisms affecting antioxidant enzymes are present in tympanosclerosis patients

The Journal of Laryngology & Otology ◽

10.1017/s0022215116008732 ◽

2016 ◽

Vol 130 (10) ◽

pp. 928-933 ◽

Cited By ~ 1

Author(s):

A Akyigit ◽

Ş Yalcin ◽

E Ö Etem ◽

İ Kaygusuz ◽

T Karlidag ◽

...

Keyword(s):

Genetic Polymorphisms ◽

Otitis Media ◽

Venous Blood ◽

Chronic Otitis Media ◽

Study Groups ◽

Control Group ◽

Genotype Distribution ◽

Control Groups ◽

Significant Difference ◽

And Control

AbstractBackground:This study investigated genetic polymorphisms affecting the inducible nitric oxide synthase, superoxide dismutase and catalase enzymes in chronic otitis media patients with and without tympanosclerosis, and the role of genetic susceptibility in the disease aetiology.Methods:A total of 162 patients who underwent surgery for chronic otitis media were divided into two study groups: a tympanosclerosis group and a chronic otitis media group. A third, the control, group comprised 188 healthy volunteers. Venous blood samples were evaluated using reverse transcriptase polymerase chain reaction.Results:There was a significant difference in GG genotype distribution of the −277A>G polymorphism in the NOS2 gene between the tympanosclerosis and control groups (p < 0.05). However, there were no significant between-group differences in the CC genotype distribution of the p.Ala16Val (C>T) polymorphism in the SOD2 gene (p > 0.05). There were significant differences in the TT genotype distribution of the −21A>T polymorphism in the CAT gene between the tympanosclerosis and control groups, and between the chronic otitis media and control groups (p < 0.05).Conclusion:These results suggest that genetic predisposition may play a role in the aetiopathogenesis of tympanosclerosis.

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The Impact of Vitamin D Supplementation as an Adjuvant Therapy on the Abundance of Treg Cells in Patients with Atopic Dermatitis

10.21203/rs.3.rs-473562/v1 ◽

2021 ◽

Author(s):

Payam Payandeh ◽

Maryam Rastin ◽

Farzaneh Iravani ◽

Maryam Khoshkhui ◽

pouran Layegh ◽

...

Keyword(s):

Vitamin D ◽

Atopic Dermatitis ◽

Immune System ◽

Treg Cells ◽

Control Group ◽

Vitamin D Levels ◽

Significant Difference ◽

And Control ◽

The Impact ◽

Experimental Group

Abstract BackgroundAtopic dermatitis (AD) is a chronic, inflammatory, and severely Pruritus cutaneous disease that has an immunologic base. The most common treatments are topical steroids, which bring many adverse side effects. Vitamin D can adjust the immune system; therefore, in this study, we investigated the effect of vitamin D on the abundance of Treg cells in AD patients. MethodsIn this interventional study, 40 subjects who completed the intervention were enrolled, including 20 AD patients (as the experimental group) and 20 healthy subjects (as the control group). Accordingly, the subjects whose vitamin D levels was less than 30 ng/ml were assigned to receive vitamin D (1000 IU) daily for a 2-month duration. The severity of AD was evaluated based on SCORAD (Scoring Atopic Dermatitis) and the amount of CD4+CD25+Foxp3+Treg cells was also assessed using Flow cytometry. ResultsAverage serum level of vitamin D before performing the treatment in the experimental (14/90±4/5) and control groups (15/95±5/0) was lower than that of after the treatment (experimental group 24/60±5/2 and control group 23/60±7/3) (P<0.001). As well, there was a significant difference between the average scores of SCORAD after the vitamin D intervention (P<0.001). The amount of Treg cells significantly increased in the experimental group (P=0.002, Diff=0.35) after performing the intervention.Conclusionit was found that besides conventional medicines, Vitamin D can be adjusted as an effective complementary therapy by modulating the immune system.Trial registration: IRCT20150716023235N13, Registered 11 Feb 2018, https://www.irct.ir

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