Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium InhabitingEuphorbia hirtaL. and Their Bactericidal Potential

The present investigation aims to evaluate biomimetic synthesis of silver nanoparticles using endophytic bacterium EH 419 inhabitingEuphorbia hirtaL. The synthesized nanoparticles were initially confirmed with change in color from the reaction mixture to brown indicating the synthesis of nanoparticles. Further confirmation was achieved with the characteristic absorption peak at 440 nm using UV-Visible spectroscopy. The synthesized silver nanoparticles were subjected to biophysical characterization using hyphenated techniques. The possible role of biomolecules in mediating the synthesis was depicted with FTIR analysis. Further crystalline nature of synthesized nanoparticles was confirmed using X-ray diffraction (XRD) with prominent diffraction peaks at 2θwhich can be indexed to the (111), (200), (220), and (311) reflections of face centered cubic structure (fcc) of metallic silver. Transmission electron microscopy (TEM) revealed morphological characteristics of synthesized silver nanoparticles to be polydisperse in nature with size ranging from 10 to 60 nm and different morphological characteristics such as spherical, oval, hexagonal, and cubic shapes. Further silver nanoparticles exhibited bactericidal activity against panel of significant pathogenic bacteria among whichPseudomonas aeruginosawas most sensitive compared to other pathogens. To the best of our knowledge, present study forms first report of bacterial endophyte inhabitingEuphorbia hirtaL. in mediating synthesizing silver nanoparticles.

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Trillium govanianum Wall. Ex. Royle rhizomes extract-medicated silver nanoparticles and their antimicrobial activity

Green Processing and Synthesis ◽

10.1515/gps-2020-0054 ◽

2020 ◽

Vol 9 (1) ◽

pp. 503-514 ◽

Cited By ~ 1

Author(s):

Khaleeq Uz-Zaman ◽

Jehan Bakht ◽

Bates Kudaibergenova Malikovna ◽

Eman R. Elsharkawy ◽

Anees Ahmed Khalil ◽

...

Keyword(s):

Silver Nanoparticles ◽

Cost Effective ◽

Face Centered Cubic ◽

Synthesis Of Nanoparticles ◽

E Coli ◽

Area Of Interest ◽

Cost Effective Technique ◽

Face Centered ◽

Trillium Govanianum ◽

Average Size

AbstractSynthesis of nanoparticles is a fast-growing area of interest in the current development in science and technology. Nanoparticles are also used in biomedical applications. Green synthesis of nanoparticles is an environmental friendly and cost-effective technique. Trillium govanianum Wall. Ex. Royle crude extract was used for the eco-friendly genesis of silver nanoparticles (AgNPs). Aromatic amines were the functional groups involved in the bio-fabrication and synthesis of the AgNPs. The production of AgNPs was established by the appearance of brown color. The manufactured AgNPs were characterized by UV-Vis spectrophotometer, X-ray diffractometer, and FTIR spectrophotometer. AgNPs were face-centered cubic in nature with an average size of 9.99 nm. The produced AgNPs (18 µL disc−1) showed substantial antibacterial (53.74, 52.75, 51.61, 43.00, 36.84, and 36.84%) and antifungal (54.05, 42.11, 41.10, 40.85, 30.55, and 29.73%) potential against the tested bacterial (X. campestris, P. aeruginosa, S. aureus, E. coli, B. subtilis, and K. pneumoniae) and fungal (A. alternaria, Paecilomyces, C. albicans, Curvularia, A. niger, and Rhizopus) strains, respectively.

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Review of Biosynthesis Silver Nanoparticles by Microbiology

Medical Science Journal for Advance Research ◽

10.46966/msjar.v1i01.7 ◽

2020 ◽

Vol 1 (01) ◽

pp. 7-11

Author(s):

Dr. Hawraa Natiq Kabroot Al-Fatlawy ◽

Khwla Abd Al Kadhem Rahi ◽

Diaa Sarhan Abd Al Zahra ◽

Samara Rahem Hudud ◽

Hussein Ali Hussein ◽

...

Keyword(s):

Silver Nanoparticles ◽

Pathogenic Bacteria ◽

Endophytic Bacterium ◽

Antibacterial Activities ◽

Salmonella Typhi ◽

Biological Synthesis ◽

Klebsiella Pneumonia ◽

Ambient Conditions ◽

Replication Process ◽

Synthesis Of Nanoparticles

Nanotechnology is a multidisciplinary field that evolved within the past few decades and played a substantial role in the environment, industry, agriculture, and pharmacology. Nanoparticles are generally classified based on their dimensionality, morphology, composition, uniformity, and agglomeration. The shape, and morphology of nanoparticles play an essential role in their functionality and toxic effect on the environment and humans. In this review, we discuss the biosynthesis of nanoparticles from microbes. For the biological synthesis of nanoparticles, microbes have been exploited all over the globe. Microbes like bacteria, fungi, and yeasts are mostly preferred for nanoparticles (NPs) synthesis because of their fast growth rate, easy cultivation, and their ability to grow at ambient conditions of temperature, pH, and pressure. Applications of Nanoparticles is a field of research with tremendous prospects for the improvement of the diagnosis and treatment of human diseases. Microbial nanoparticles are found to have vigorous antibacterial activities. The nanoparticles' efficiency is probably due to their larger surface area for enhanced interaction with the micro-organisms. Nanoparticles adhere to the cell membrane and further penetrate inside by interacting with DNA, thereby interfering with the replication process or may attack the respiratory chain of pathogens. A similar bactericidal mechanism of silver nanoparticles obtained from endophytic bacterium Bacillus cereus was observed against pathogenic bacteria like Salmonella typhi, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Pseudomonas aeruginosa.

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Synthesis and Characterization of Silver Nanoparticles from Ashyranthus aspera Extract for Antimicrobial Activity Studies

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v24i7.6 ◽

2020 ◽

Vol 24 (7) ◽

pp. 1161-1167

Author(s):

V. Swetha ◽

S Lavanya ◽

G. Sabeena ◽

E. Pushpalaksmi ◽

Samraj J. Jenson ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Antimicrobial Agents ◽

Exothermic Reaction ◽

Visual Observation ◽

Metallic Silver ◽

Face Centered Cubic ◽

Leaf Extracts ◽

Synthesis Of Nanoparticles ◽

Potential Applications

Development of biologically inspired experimental processes for the synthesis of nanoparticles is evolving into an important branch of nanotechnology. Plant-mediated synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. In the present study, we were synthesized silver (Ag) nanoparticles using aqueous extracts of fresh leaves of Ashyranthus aspera medicinal plants as bio-reducing agents. UV-Vis spectrometer used to monitor the reduction of Ag ions and the formation of AgNPs in the medium. UV-Vis spectra and visual observation showed that the color of the fresh leaf extracts of Ashyranthus aspera turned into grayish-brown respectively, after treatment with Ag precursors. XRD and SEM have been used to investigate the morphology of prepared AgNPs. The peaks in the XRD pattern are associated with that of the Face-Centered-Cubic (FCC) form of metallic silver. TGA/DTA results associated with weight loss and exothermic reaction due to the desorption of chemisorbed water. FTIR was performed to identify the functional groups which form a layer covering AgNPs and stabilize the AgNPs in the medium. Moreover, silver nanoparticles using aqueous leaf extracts of Ashyranthus aspera were separately tested for their antibacterial activity against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Enterobacter). The results showed that the bacterial growth was inhibited by the extracts containing AgNPs Nanoparticles. The biosynthesized nanoparticle was prepared from Ashyranthus aspera leaf extracts exhibits potential applications as broad-spectrum antimicrobial agents Keywords: Ashyranthus aspera, Silver Nanoparticles, Plant extracts, Bacteria, Antibacterial activity.

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Fabrication of Silver Nanoparticles and its Films and their Characterization of Structure and Electrical Conductivity

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.896.341 ◽

2014 ◽

Vol 896 ◽

pp. 341-346 ◽

Cited By ~ 1

Author(s):

Markus Diantoro ◽

Alif Fitria Chasanah ◽

Nasikhudin ◽

Nandang Mufti ◽

Abdulloh Fuad

Keyword(s):

Electrical Conductivity ◽

Silver Nanoparticles ◽

Nano Particles ◽

Metallic Silver ◽

Cubic Phase ◽

Face Centered Cubic ◽

X Ray ◽

Average Grain Size ◽

Face Centered

Based on our previous work of MSA influence on silver nano particles formation we have performed a series of research related to the preparation and characterization of silver nanoparticles which synthesized through coprecipitation process. The silver nano particles was transformed into silver nanoparticles films by spin coating deposition method. The silver nanoparticles were prepared from silver acetate as precursor, reductant agent phenylhydrazine, and dodecylamine as stabilizer. X-Ray Fluorescence (XRF), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX), and Electric Four Point Probe have been used to characterize the silver nanoparticles as well as the films. The morphology and the crystal structure have been determined by means of XRD and SEM. The average grain size of silver nanoparticles is found to be 22 nm. The peaks in XRD pattern are in good agreement with that of face-centered-cubic phase of metallic silver. The electrical conductivity films were confirmed in the range of conducting material. The highest conductivity was reached at 3,88 x 109 S/cm associated to 1600 rpm of spin coater rotation.

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A green approach for the biosynthesis of powdered silver nanoparticles using leaf extract of Trigonella foenum-graecum L.: Characterization and antibacterial evaluation

Bangladesh Journal of Botany ◽

10.3329/bjb.v48i3.47722 ◽

2019 ◽

Vol 48 (3) ◽

pp. 427-436

Author(s):

T Rajkumar ◽

Jayanta Kumar Patra

Keyword(s):

Silver Nanoparticles ◽

Food Packaging ◽

Pathogenic Bacteria ◽

Leaf Extract ◽

Visible Spectrum ◽

Face Centered Cubic ◽

Green Approach ◽

Zones Of Inhibition ◽

Face Centered ◽

Trigonella Foenum Graecum L

Silver nanoparticles (AgNPs) were synthesized using aqueous leaf extract of Trigonella foenumgraecum as reducing and stabilizing agent. The ultraviolet-visible spectrum showed absorption peak at 480 nm. XRD pattern indicates the formation of face-centered cubic structure of silver nanoparticles. FESEM images indicate the presence of spherical silver nanoparticles with the particle size of ~90 nm. FTIR indicates that the participation of different functional groups present in the biomolecules is responsible for both reducing and stabilizing the formation of nanoparticles. The synthesized AgNPs demonstrated positive antibacterial activity against two different foodborne pathogenic bacteria (Escherichia coli O157:H7 ATCC 35150, Staphylococcus aureus ATCC 13565) with zones of inhibition of 9.20 ± 0.18 and 9.34 ± 0.11, respectively and MIC and MBC of 100 and >100 μg/ml, respectively. The synthesized AgNPs could serve as a candidate for development of antibacterial drugs or additive in the food packaging system for its application in medicine, cosmetics and food sector industries.

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Green Synthesis of Silver Nanoparticles by Allamanda cathartica L. Leaf Extract and Evaluation for Antimicrobial Activity

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2013.6.4.9 ◽

2013 ◽

Vol 6 (4) ◽

pp. 2260-2268

Author(s):

M. Linga Rao ◽

Bhumi G ◽

Savithramma N

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Fusarium Species ◽

Inhibition Zone ◽

Fungal Species ◽

Green Technology ◽

Metallic Silver ◽

Synthesis Of Nanoparticles ◽

Aqueous Leaf Extract

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent. The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals. In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica. The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM). The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size. These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species. These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

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Green Synthesis and Characterization of Pullulan Mediated Silver Nanoparticles through Ultraviolet Irradiation

Materials ◽

10.3390/ma12152382 ◽

2019 ◽

Vol 12 (15) ◽

pp. 2382 ◽

Cited By ~ 7

Author(s):

Muhammad Jamshed Khan ◽

Suriya Kumari ◽

Kamyar Shameli ◽

Jinap Selamat ◽

Awis Qurni Sazili

Keyword(s):

Silver Nanoparticles ◽

Uv Irradiation ◽

Irradiation Time ◽

Edible Films ◽

Crystallographic Structure ◽

Face Centered Cubic ◽

Selected Area Electron Diffraction ◽

Vis Spectroscopy ◽

Face Centered ◽

Average Lattice

Nanoparticles (NPs) are, frequently, being utilized in multi-dimensional enterprises. Silver nanoparticles (AgNPs) have attracted researchers in the last decade due to their exceptional efficacy at very low volume and stability at higher temperatures. Due to certain limitations of the chemical method of synthesis, AgNPs can be obtained by physical methods including sun rays, microwaves and ultraviolet (UV) radiation. In the current study, the synthesis of pullulan mediated silver nanoparticles (P-AgNPs) was achieved through ultraviolet (UV) irradiation, with a wavelength of 365 nm, for 96 h. P-AgNPs were formed after 24 h of UV-irradiation time and expressed spectra maxima as 415 nm, after 96 h, in UV-vis spectroscopy. The crystallographic structure was “face centered cubic (fcc)” as confirmed by powder X-ray diffraction (PXRD). Furthermore, high resolution transmission electron microscopy (HRTEM) proved that P-AgNPs were covered with a thin layer of pullulan, with a mean crystalline size of 6.02 ± 2.37. The average lattice fringe spacing of nanoparticles was confirmed as 0.235 nm with quasi-spherical characteristics, by selected area electron diffraction (SAED) analysis. These green synthesized P-AgNPs can be utilized efficiently, as an active food and meat preservative, when incorporated into the edible films.

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Green Synthesis and Catalytic Activity of Silver Nanoparticles Based on Piper chaba Stem Extracts

Nanomaterials ◽

10.3390/nano10091777 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1777 ◽

Cited By ~ 2

Author(s):

Md. Mahiuddin ◽

Prianka Saha ◽

Bungo Ochiai

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Green Synthesis ◽

Ftir Spectroscopy ◽

Visual Observation ◽

Face Centered Cubic ◽

X Ray ◽

Vis Spectroscopy ◽

Face Centered ◽

Average Size

A green synthesis of silver nanoparticles (AgNPs) was conducted using the stem extract of Piper chaba, which is a plant abundantly growing in South and Southeast Asia. The synthesis was carried out at different reaction conditions, i.e., reaction temperature, concentrations of the extract and silver nitrate, reaction time, and pH. The synthesized AgNPs were characterized by visual observation, ultraviolet–visible (UV-vis) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), energy dispersive x-ray (EDX), and Fourier transform infrared (FTIR) spectroscopy. The characterization results revealed that AgNPs were uniformly dispersed and exhibited a moderate size distribution. They were mostly spherical crystals with face-centered cubic structures and an average size of 19 nm. The FTIR spectroscopy and DLS analysis indicated that the phytochemicals capping the surface of AgNPs stabilize the dispersion through anionic repulsion. The synthesized AgNPs effectively catalyzed the reduction of 4-nitrophenol (4-NP) and degradation of methylene blue (MB) in the presence of sodium borohydride.

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Green Synthesis of Silver Nanoparticles using Salacca zalacca Extract as Reducing Agent and It’s Antibacterial Activity

Asian Journal of Chemistry ◽

10.14233/ajchem.2019.22238 ◽

2019 ◽

Vol 31 (12) ◽

pp. 2804-2810

Author(s):

Anti Kolonial Prodjosantoso ◽

Oktanio Sigit Prawoko ◽

Maximus Pranjoto Utomo ◽

Lis Permana Sari

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Staphylococcus Epidermidis ◽

Silver Ions ◽

Reduction Reaction ◽

Face Centered Cubic ◽

Gram Positive Bacteria ◽

Transmission Electron ◽

Face Centered

In this article, the synthesis of silver nanoparticles through a reduction reaction process using Salacca zalacca extract is reported. The AgNPs were characterized using X-ray diffraction, transmission electron microscopy, Fourier transform infrared and UV-visible spectrophotometry methods. The AgNPs antibacterial activity was determined against of Gram-positive bacteria (Staphylococcus epidermidis) and Gram-negative bacteria (Escherichia coli). The main functional groups contained in Salacca zalacca extract are carbonyl, hydroxyl and nitrile groups, which are believed to reduce the silver ions to metal. The surface plasmon resonance values of brownish red AgNPs are in the range of 410 nm to 460 nm. The structure of AgNPs is face centered cubic (FCC). The diameter of silver nanoparticles crystallite is 14.2 ± 2.6 nm. The AgNPs growth inhibition zones of Escherichia coli and Staphylococcus epidermidis are 9.6 mm and 9.2 mm, respectively.

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Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

Scientific Reports ◽

10.1038/s41598-019-50906-6 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 13

Author(s):

Sudip Some ◽

Onur Bulut ◽

Kinkar Biswas ◽

Anoop Kumar ◽

Anupam Roy ◽

...

Keyword(s):

Silver Nanoparticles ◽

Bombyx Mori ◽

Leaf Extract ◽

Silver Ions ◽

Average Diameter ◽

Face Centered Cubic ◽

Tem Analysis ◽

Beneficial Effects ◽

Selected Area Electron Diffraction ◽

Face Centered

Abstract Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

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