scholarly journals Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of Soybean

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Asmiaty Sahur ◽  
Ambo Ala ◽  
Baharuddin Patandjengi ◽  
Elkawakib Syam’un
Keyword(s):  
Nitrogen Fixation ◽  
Morphological Character ◽  
Nitrogenase Activity ◽  
Growth And Yield ◽  
Good Growth ◽  
Plant Host ◽  
Seed Inoculation ◽  
Soybean Plants ◽  
Almost All ◽  
Free Media

The present study was initiated to determine whether isolates from soil and roots of soybean plants can express nitrogenase activity when grown in the absence of plant host. The study was conducted to answer the question “can benefit gained by” the interaction between Actinomycetes and Rhizobium symbiosis with legume. Thirty-five isolates identified as Rhizobium and twenty-one Actinomycetes were isolated from the rhizosphere of soybean plants and identified by morphological character, biochemical content identified. Fifty-six isolates were tested for their capabilities of N2fixation and siderophore production. The isolated rhizobacteria were grown in N-free media, and twelve of them showed a good growth on the Burk’s N-free media. Almost all strains produced siderophores; however, the production level was very low, and only the strain AK 10 released considerable amounts of this metabolite. One strain of Actinomycetes was selected to test their interactions with Rhizobium. Coinoculation of Actinomycetes and Rhizobium produced synergic benefits on plant growth and get protection from the production of siderophore.

High-temperature-adaptedAzospirillum brasilensestrains: growth and interaction response on associative nitrogen fixation, mineral uptake and yield of cheena (Panicum miliaceumL.) genotypes in calcareous soil

1988 ◽  
Vol 110 (2) ◽  
pp. 321-329 ◽  
Author(s):  
R. Rai
Keyword(s):  
Nitrogen Fixation ◽  
High Temperature ◽  
Calcareous Soil ◽  
Nitrogenase Activity ◽  
Dry Weight ◽  
Cross Resistance ◽  
Mineral Uptake ◽  
Associative Nitrogen Fixation ◽  
Seed Inoculation ◽  
Resistance To Penicillin

SummaryHigh-temperature-adapted strains RAU 1, RAU 2 and RAU 3 ofAzospirillum brasilenseC 7 were isolated from stepwise transfer to higher temperature (30 to 42 °C). One of the strains (RAU 1) showed more growth, greater nitrogenase and hydrogenase activities at 30 and 42 °C than parental and other temperature-adapted strains. This strain also showed growth and more nitrogenase activity from pH 6·5 to 8·0. Strain RAU 1 showed cross-resistance to penicillin (300/µg/ml) but not to streptomycin, kanamycin, viomycin and polymixin B at 30 and 42 °C. It was demonstrated in field plots in calcareous soil that seed inoculation with RAU 1 enhanced mineral uptake of cheena. Inoculation with RAU 1 led to a significant increase in associative nitrogen fixation, dry weight of roots, grain and straw yield of cheena compared with the uninoculated control with or without applied N, but the effect of seed inoculation with high-temperature-adapted strains was variable with different genotypes of cheena.

scholarly journals Nitrogen-fixation and phosphate-solubilization bacteria isolated from alluvial and latosol soil paddy field

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
IKHWANI IKHWANI ◽  
NONON SARIBANON ◽  
TATANG MITRA SETIA ◽  
ERNY YUNIARTI ◽  
Jumakir Jumakir
Keyword(s):  
Nitrogen Fixation ◽  
Molecular Characterization ◽  
Paddy Field ◽  
Phosphate Solubilization ◽  
Nitrogenase Activity ◽  
Alluvial Soil ◽  
Soil Biology ◽  
Bacteria Growth ◽  
Free Media ◽  
Biology Laboratory

Abstract. Ikhwani, Saribanon N, Setia TM, Yuniarti E, Jumakir. 2021. Nitrogen-fixation and phosphate-solubilization bacteria isolated from alluvial and latosol soil paddy field. Biodiversitas 22: 4722-4730. This study aims to isolate, select and identify molecular characterization of bacteria from alluvial and latosol soil paddy field. This study has been conducted from February-June 2020 in the Soil Biology Laboratory, ICALRRD, Bogor. Alluvial and latosol soil samples were selected from Pusakanagara and Muara experimental gardens, Muara district. The result showed that bacteria growth in N-free media was higher from latosol soil as compared to alluvial soil. The higher nitrogenase activity isolates, could form ethylene gas concentration 1.1.E + 09 µmol/mL/hours, 1.7.E + 09 µmol/mL/hours and 1.3.E + 09 µmol/mL/hours from  alluvial 6, latosol 4, and latosol 9.  Three isolates have great PSB performances, i.e. isolates No. 4.2, 2.1 and 2 could dissolve 14.398 mg l-1, 12.648 mg l-1 and 12.145 mg l-1 of phosphate, from latosol soil. The highest N-fixing and solubilization capacity identified by molecular characterization i.e latosol 4, 4.2 and alluvial 7.1, alluvial-1.11. The phylogeny analyzed the presence of bacterial isolates in two distinct groups. Latosol 3.1 and latosol 4.2 isolates clustered in the same as the genus Lysinibacillus bacteria. The Alluvial 7.1 bacterial isolate showed similarities by Fictibacillus sp. and alluvial 1.2 isolate showed similarities by the genus Bacillus sp.

scholarly journals Effect of Sowing Depth on Nodulation, Nitrogen Fixation, Root and Hypocotyl Growth, and Yield in Groundnut (Arachis hypogaea)

1987 ◽  
Vol 23 (3) ◽  
pp. 283-291 ◽  
Author(s):  
P. T. C. Nambiar ◽  
B. Srinivasa Rao
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Field Experiments ◽  
Nitrogenase Activity ◽  
Root Nodules ◽  
Growth And Yield ◽  
Fixed Nitrogen ◽  
Hypocotyl Growth ◽  
Hypocotyl Length ◽  
Sowing Depth

SUMMARYHypocotyl length in groundnut is a function of sowing depth. In field experiments deep sowing increased the mass of hypocotyl but decreased that of roots, pods and haulm. Few nodules were formed on the hypocotyls of plants from shallow sown seeds (4–5 cm deep). More hypocotyl nodules occurred on Virginia types when deep sown but the number and activity of nodules on the roots decreased. Nodules on the hypocotyl appeared later and fixed less nitrogen than root nodules. Although hypocotyl nodules fixed nitrogen during the later stages of plant growth, this activity could not compensate for the loss in nitrogenase activity due to deeper sowing. Deeper sowing also resulted in decreased pod yields.

scholarly journals Physiological and biochemical responses of soybean plants inoculated with Arbuscular mycorrhizal fungi and Bradyrhizobium under drought stress

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mohamed S. Sheteiwy ◽  
Dina Fathi Ismail Ali ◽  
You-Cai Xiong ◽  
Marian Brestic ◽  
Milan Skalicky ◽  
...  
Keyword(s):  
Drought Stress ◽  
Arbuscular Mycorrhizal Fungi ◽  
Secondary Metabolism ◽  
Mycorrhizal Fungi ◽  
Plant Biomass ◽  
Arbuscular Mycorrhizal ◽  
Growth And Yield ◽  
Relative Expression ◽  
Reverse Trend ◽  
Soybean Plants

Abstract Background The present study aims to study the effects of biofertilizers potential of Arbuscular Mycorrhizal Fungi (AMF) and Bradyrhizobium japonicum (B. japonicum) strains on yield and growth of drought stressed soybean (Giza 111) plants at early pod stage (50 days from sowing, R3) and seed development stage (90 days from sowing, R5). Results Highest plant biomass, leaf chlorophyll content, nodulation, and grain yield were observed in the unstressed plants as compared with water stressed-plants at R3 and R5 stages. At soil rhizosphere level, AMF and B. japonicum treatments improved bacterial counts and the activities of the enzymes (dehydrogenase and phosphatase) under well-watered and drought stress conditions. Irrespective of the drought effects, AMF and B. japonicum treatments improved the growth and yield of soybean under both drought (restrained irrigation) and adequately-watered conditions as compared with untreated plants. The current study revealed that AMF and B. japonicum improved catalase (CAT) and peroxidase (POD) in the seeds, and a reverse trend was observed in case of malonaldehyde (MDA) and proline under drought stress. The relative expression of the CAT and POD genes was up-regulated by the application of biofertilizers treatments under drought stress condition. Interestingly a reverse trend was observed in the case of the relative expression of the genes involved in the proline metabolism such as P5CS, P5CR, PDH, and P5CDH under the same conditions. The present study suggests that biofertilizers diminished the inhibitory effect of drought stress on cell development and resulted in a shorter time for DNA accumulation and the cycle of cell division. There were notable changes in the activities of enzymes involved in the secondary metabolism and expression levels of GmSPS1, GmSuSy, and GmC-INV in the plants treated with biofertilizers and exposed to the drought stress at both R3 and R5 stages. These changes in the activities of secondary metabolism and their transcriptional levels caused by biofertilizers may contribute to increasing soybean tolerance to drought stress. Conclusions The results of this study suggest that application of biofertilizers to soybean plants is a promising approach to alleviate drought stress effects on growth performance of soybean plants. The integrated application of biofertilizers may help to obtain improved resilience of the agro ecosystems to adverse impacts of climate change and help to improve soil fertility and plant growth under drought stress.

scholarly journals Effects of Biofertilizer Produced from Bradyrhizobium and Streptomyces griseoflavus on Plant Growth, Nodulation, Nitrogen Fixation, Nutrient Uptake, and Seed Yield of Mung Bean, Cowpea, and Soybean

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 77 ◽  
Author(s):  
Aung Zaw Htwe ◽  
Seinn Moh Moh ◽  
Khin Myat Soe ◽  
Kyi Moe ◽  
Takeo Yamakawa
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Seed Yield ◽  
Mung Bean ◽  
Growth And Yield ◽  
Vegetable Crops ◽  
N Application ◽  
Npk Uptake ◽  
Phosphorus And Potassium ◽  
Leguminous Crops

The use of biofertilizers is important for sustainable agriculture, and the use of nodule bacteria and endophytic actinomycetes is an attractive way to enhance plant growth and yield. This study tested the effects of a biofertilizer produced from Bradyrhizobium strains and Streptomyces griseoflavus on leguminous, cereal, and vegetable crops. Nitrogen fixation was measured using the acetylene reduction assay. Under N-limited or N-supplemented conditions, the biofertilizer significantly promoted the shoot and root growth of mung bean, cowpea, and soybean compared with the control. Therefore, the biofertilizer used in this study was effective in mung bean, cowpea, and soybean regardless of N application. In this study, significant increments in plant growth, nodulation, nitrogen fixation, nitrogen, phosphorus, and potassium (NPK) uptake, and seed yield were found in mung beans and soybeans. Therefore, Bradyrhizobium japonicum SAY3-7 plus Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus are effective bacteria that can be used together as biofertilizer for the production of economically important leguminous crops, especially soybean and mung bean. The biofertilizer produced from Bradyrhizobium and S. griseoflavus P4 will be useful for both soybean and mung bean production.

scholarly journals Paraburkholderia phymatum Homocitrate Synthase NifV Plays a Key Role for Nitrogenase Activity during Symbiosis with Papilionoids and in Free-Living Growth Conditions

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 952
Author(s):  
Paula Bellés-Sancho ◽  
Martina Lardi ◽  
Yilei Liu ◽  
Sebastian Hug ◽  
Marta Adriana Pinto-Carbó ◽  
...  
Keyword(s):  
Nitrogenase Activity ◽  
Root Nodules ◽  
Growth Conditions ◽  
Lysine Biosynthesis ◽  
Metabolome Analysis ◽  
Plant Host ◽  
Free Living ◽  
Bacterial Enzyme ◽  
Homocitrate Synthase ◽  
Iron Molybdenum Cofactor

Homocitrate is an essential component of the iron-molybdenum cofactor of nitrogenase, the bacterial enzyme that catalyzes the reduction of dinitrogen (N2) to ammonia. In nitrogen-fixing and nodulating alpha-rhizobia, homocitrate is usually provided to bacteroids in root nodules by their plant host. In contrast, non-nodulating free-living diazotrophs encode the homocitrate synthase (NifV) and reduce N2 in nitrogen-limiting free-living conditions. Paraburkholderia phymatum STM815 is a beta-rhizobial strain, which can enter symbiosis with a broad range of legumes, including papilionoids and mimosoids. In contrast to most alpha-rhizobia, which lack nifV, P. phymatum harbors a copy of nifV on its symbiotic plasmid. We show here that P. phymatum nifV is essential for nitrogenase activity both in root nodules of papilionoid plants and in free-living growth conditions. Notably, nifV was dispensable in nodules of Mimosa pudica despite the fact that the gene was highly expressed during symbiosis with all tested papilionoid and mimosoid plants. A metabolome analysis of papilionoid and mimosoid root nodules infected with the P. phymatum wild-type strain revealed that among the approximately 400 measured metabolites, homocitrate and other metabolites involved in lysine biosynthesis and degradation have accumulated in all plant nodules compared to uninfected roots, suggesting an important role of these metabolites during symbiosis.

scholarly journals Growth and yield performance of soybean with the application of Bradyrhyzobium inoculant via furrow and seed

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2387
Author(s):  
Santiel Alves Vieira Neto ◽  
Fábio Ribeiro Pires ◽  
João Carlos Madalão ◽  
Douglas Gomes Viana ◽  
Carlos César Evangelista de Menezes ◽  
...  
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
Agricultural Production ◽  
Aerial Part ◽  
Dry Matter ◽  
Growth And Yield ◽  
Recommended Dose ◽  
Yield Performance ◽  
Seed Inoculation ◽  
Dry Matter Weight

Given the high costs of agricultural production, especially due to the price of fertilisers, particularly nitrogen, the use of inoculants to supply nitrogen to soybean crops is a widely recommended practice. The objective of this study was to evaluate the feasibility of applying inoculants through seed and planting furrow in soil previously cultivated with soybean and Brazilian native “cerrado” biome soil under greenhouse conditions. Seven treatments were tested: 1) inoculation via seed (inoculant + fungicide + micronutrient), 2) treatment via seed (fungicide + micronutrient), 3) control (only seed), 4) inoculation via furrow-dose 1 (recommended dose), 5) inoculation via furrow-dose 2 (twice the recommended dose), 6) inoculation via furrow-dose 3 (three times the recommended dose) and 7) inoculation via furrow-dose 1 + seed inoculation. We evaluated plant height, fresh and dry matter weight of the aerial part and nodules, number of total, viable and non-viable nodules, number of pods per plant and grain yield. Inoculation was more effective when used in cerrado soil, but soybean performance in treatments without inoculation was higher in previously cultivated soil. Application through furrow proved to be a viable practice due to the similarity of the results obtained with the traditional application by seed.

scholarly journals Effects of selenium (Se) application and arbuscular mycorrhizal (AMF) inoculation on soybean (Glycine max) and forage grass (Urochloa decumbens) development in oxisol

2019 ◽  
Vol 13 ((03) 2019) ◽  
pp. 380-385 ◽  
Author(s):  
Soraya Marx Bamberg ◽  
Silvio Junio Ramos ◽  
Marco Aurelio Carbone Carneiro ◽  
José Oswaldo Siqueira
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Fold Increase ◽  
Tropical Soils ◽  
Fertilizer Application ◽  
Growth And Yield ◽  
Forage Grass ◽  
Urochloa Decumbens ◽  
Soybean Plants ◽  
Amf Inoculation

Fertilizer application can enhance the nutritional value of plants, such effects being influenced by the presence of arbuscular mycorrhizal fungi (AMF). Nutrients × AMF interactions are well-known for variety of elements but very little has been addressed on biofortification of selenium (Se) in plants grown in tropical soils. The purpose of this study was to evaluate the effect of Se application and AMF inoculation on growth and micronutrient contents on soybean plants as forage grass. The experiments were conducted in a completely randomized factorial design with five Se doses (0.0, 0.5, 1.0, 2.0 and 3.0 mg kg-1 for soybean plants, and 0.0, 0.5, 1.0, 3.0 and 6.0 mg kg-1 for forage plants), with and without AMF inoculation in three replicates. The results showed that soil Se had only slight effect on soybean growth but it caused a two-fold increase on grain yield. However, the growth of forage grass was enhanced by Se application when AMF was present. The AMF inoculation reduced benefit for soybean growth and yield but marked positive effect on forage grass at high doses of Se. Selenium contents in both plants were increased by its application in soil, being such effect proportional to soil applied doses. Selenium application and AMF inoculation had marked effects on micronutrients contents in both soybean plants and forage grass and they may contribute to Se and micronutrient biofortification.

Studies on compatibility of nitrogen fixation by high-temperature-adapted Rhizobium strains and Vigna radiata genotypes at two moisture levels in calcareous soil

1983 ◽  
Vol 101 (2) ◽  
pp. 377-381 ◽  
Author(s):  
R. Rai ◽  
V. Prasad
Keyword(s):  
Nitrogen Fixation ◽  
High Temperature ◽  
Grain Yield ◽  
Vigna Radiata ◽  
Calcareous Soil ◽  
Nitrogenase Activity ◽  
Summer Season ◽  
Green Gram ◽  
Rhizobium Strains

SUMMARYRhizobium strains adapted to high temperature, and genotypes of green gram, were used to study the symbiotic N2-fixation in a summer season at two moisture levels in calcareous soil. Different interactions between strains and genotypes were observedatthe two moisture levels. At both moisture levels, strain S4 with the green gram genotype S8 showed the greatest grain yield, nitrogenase activity, leghaemoglobin and ethanolsoluble carbohydrate of nodules.

scholarly journals Effect of Sulphur and GA3 on the Growth and Yield of Onion

2013 ◽  
Vol 21 (1-2) ◽  
pp. 57-63 ◽  
Author(s):  
MHA Rashid
Keyword(s):  
Dry Matter ◽  
Matter Content ◽  
Growth And Yield ◽  
Control Treatment ◽  
Dry Matter Content ◽  
Number Of Leaves ◽  
Four Levels ◽  
Experimental Findings ◽  
Almost All ◽  
Bulb Yield

An experiment was conducted at the Horticulture Farm of the Bangladesh Agricultural University, Mymensingh to evaluate the effects of sulphur and GA3 on the growth and yield performance of onion cv. BARI Peaj-1. The experiment included four levels of sulphur viz., 0 (control), 15, 30 and 45 kg/ha and four concentrations of GA3 viz., 0 (control), 50, 75, 100 ppm. The experimental findings revealed that sulphur and GA3 had significant influence on plant height, number of leaves per plant, bulb diameter and length, individual bulb weight, splitted and rotten bulb, bulb dry matter content and bulb yield. The highest bulb yield (13.85 t/ha) was recorded from 30 kg S/ha, while the lowest bulb yield (11.20 t/ha) was obtained from control. Most of the parameters showed increasing trend with the higher concentration of GA3. Application of GA3 @ 100 ppm gave the maximum bulb yield (15.23 t/ha), while the minimum value (10.10 t/ha) was observed from control. Almost all the parameters were significantly influenced by combined treatments of sulphur and GA3 except bulb length of onion. The maximum bulb dry matter content (13.50%) and bulb yield (17.10 t/ha) were produced from the application of sulphur @ 30 kg/ha with 100ppm GA3, while the minimum bulb dry matter content (9.23%) and bulb yield (9.33 t/ha) were recorded from control treatment of sulphur with GA3.DOI: http://dx.doi.org/10.3329/pa.v21i1-2.16749 Progress. Agric. 21(1 & 2): 57 - 63, 2010

Sign in / Sign up

Export Citation Format

Share Document