The Role of Angiogenesis Factors in the Formation of Vascular Changes in Scleroderma by Assessment of the Concentrations of VEGF and sVEGFR2 in Blood Serum and Tear Fluid

Systemic sclerosis (SSc) is a connective tissue disorder characterized by tissue hypoxia, excessive fibrosis of skin and internal organs, and angiogenesis imbalance. The aim of the study was to evaluate in SSc patients the association between the retinal microcirculation disturbances and the presence of peripheral trophic changes and to determine the role of angiogenesis factors in the formation of vascular changes in scleroderma. Twenty-five SSc patients and 25 age- and sex-matched healthy controls were included to the study. Assay of vascular endothelial growth factor (VEGF) and soluble VEGF receptor-2 (sVEGFR-2) in blood serum and tears was done for all patients and controls using enzyme-linked immunosorbent assay. Retinal blood circulation was investigated with fluorescein angiography (FA) in the SSc patients only. In our research, proportion of mainly hypertensive patients presenting with a large spectrum of retinal microvascular lesions was 72%, while proportion of patients with skin microvascular lesions within distal phalanxes of fingers and toes was 76%. We noticed that patients with pathological changes in the FA examination had finger ulcerations significantly more often than patients without changes in the eye fundus. There were no statistically significant differences in the serum concentration of VEGF and sVEGFR2 between subjects in both analyzed groups. Analysis of lower levels of VEGF (p=<0.001) and sVEGFR-2 (p=<0.001) in blood serum accompanied by simultaneous higher levels of VEGF/sVEGFR-2 ratio in tears of SSc patients, as compared with the control group, indicates the superiority of proangiogenic factors in patients’ tears.

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Prognostic role of metabolic risk factors (hyperhomocysteinemia) in the development of chronic heart failure

Patologiya krovoobrashcheniya i kardiokhirurgiya ◽

10.21688/1681-3472-2014-1-20-25 ◽

2015 ◽

Vol 18 (1) ◽

pp. 20

Author(s):

Ye. N. Berezikova ◽

M. G. Pustovetova ◽

S. N. Shilov ◽

A. V. Yefremov ◽

A. T. Teplyakov ◽

...

Keyword(s):

Heart Failure ◽

Chronic Heart Failure ◽

Blood Serum ◽

Homocysteine Level ◽

Functional Class ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Relationship Of ◽

The Relationship

The aim of the study was to assess the relationship of homocysteine levels in the blood serum with the severity and nature of chronic heart failure (CHF) in patients with coronary heart disease. 94 patients with CHF were examined. The control group included 32 patients without cardiovascular disorders. At baseline and after 12 months of observation the homocysteine levels in the blood serum were determined by enzyme-linked immunosorbent assay. Correlative relationship of hyperhomocysteinemia with ischemic myocardial remodeling and with reduced inotropic function in CHF patients was observed. The homocysteine level in the blood serum of CHF patients significantly exceeded that of the control group and moderately increased with the progression of the disease severity of functional class. In the group with an unfavorable course of CHF the baseline homocysteine level proved to have the greatest value as compared to that in the group with a favorable course. In patients with a favorable course of CHF the homocysteinemia level tended to decrease towards the end of prospective study, whereas in patients with an unfavorable course, on the contrary, hyperhomocysteinemia persisted (p<0.01). In case the hyperhomocysteinemia baseline exceeds 18.5 mkmol/L (ROC-AreaSE = 0.860.04, sensitivity 71 %, specificity 90%), the severity and characteristics of the ischemic CHF could be most likely predicted. Thus, hyperhomocysteinemia is related with the severity and nature of CHF. Determining the level of homocysteine in the blood serum can be recommended for early prediction of the severity and nature of CHF.

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P0492ICAM-1 AND VEGF AS BIOMARKERS FOR PREDICTION LUPUS NEPHRITIS FLARE

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfaa142.p0492 ◽

2020 ◽

Vol 35 (Supplement_3) ◽

Author(s):

Konstantin Chyzh ◽

Tatyana Ryabceva ◽

Nikolay Soroka ◽

Pavel Viarshynin

Keyword(s):

Lupus Nephritis ◽

Blood Serum ◽

Lupus Erythematosus ◽

Roc Analysis ◽

Systemic Disease ◽

Intercellular Adhesion Molecule ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Endothelial Growth Factor ◽

Serum And Urine

Abstract Background and Aims Systemic lupus erythematosus (SLE) is autoimmune systemic disease of the connective tissue and characterized by the development of severe complications associated with damage to vital organs, in particular the kidneys. One of the important issues is an adequate assessment of the current activity and the objective prediction of the lupus nephritis (LN) flare. For this purpose molecular biomarkers are being searched. The work aim is the analyzing role of the intercellular adhesion molecule (ICAM-1) and vascular endothelial growth factor (VEGF) in the serum and urine of patients with SLE as a biomarker for lupus nephritis flare. Method The study group consisted of patients with SLE with LN (n = 23). The control group included patients with SLE without renal pathology (n = 13). The concentration of ICAM-1 and VEGF molecules was determined in blood serum and urine by enzyme-linked immunosorbent assay. Results The median concentration of ICAM-1in serum in patients with LN is 822.41 (580.50; 1300.95) ng/ml. That is two times higher than this indicator in the control group (378.97 (356.47; 439.52) ng/ml). The concentration of the VEGF molecule in serum in the group of LN is 91.99 (74.18; 132.58) ng/ml. That is three times higher than in the control group (31.13 (22.32; 132.58) ng/ml). An increase in the blood serum concentration of patients with SLE ICAM ≥577.7 ng/ml will indicate the presence of LN with a sensitivity of 82.61% and a specificity of 83.33%. ROC analysis showed the AUC coefficient is 0.927 (p = 0.0001). An increase in the blood serum in VEGF concentration of ≥66.43 ng/ml in patients with SLE will indicate the presence of LN with a sensitivity of 80.00% and a specificity of 83.33%. ROC analysis showed that the AUC coefficient is 0.916 (p = 0.006). Conclusion The results allow to conclude that it is possible to use the ICAM and VEGF concentration in serum and urine as early molecular markers of the LN and LN flare in patients with SLE.

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Evaluation of plasma VEGF and sVEGFR-1 levels in patients with diabetes mellitus receiving insulin treatment

Turkish Journal of Biochemistry ◽

10.1515/tjb-2017-0348 ◽

2019 ◽

Vol 44 (2) ◽

pp. 182-188

Author(s):

Ismail Erturk ◽

Erdim Sertoglu ◽

Fatih Yesildal ◽

Ramazan Acar ◽

Kenan Saglam ◽

...

Keyword(s):

Diabetes Mellitus ◽

Insulin Treatment ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Vegf Receptor ◽

Vascular Endothelial ◽

Patients With Diabetes ◽

Healthy Control ◽

Endothelial Growth Factor

Abstract Background Diabetes mellitus (DM) is a multifactorial chronic disease, in which patients need to be treated with insulin in some conditions. Capillary growth is regulated by growth factors like vascular endothelial growth factor (VEGF) and endogenous inhibitors such as the splice variant of VEGF receptor-1 (sVEGFR-1). We aimed to show the levels and the clinical significance of VEGF, sVEGFR-1 in patients with DM on insulin treatment. Materials and methods A total of 83 subjects consisting of patients with the diagnosis of DM (n=47) and healthy control (n=36) were included the study. Plasma levels of VEGF and sVEGFR-1, were measured using the enzyme-linked immunosorbent assay method. Results The average sVEGFR-1 levels of DM group was significantly higher than the control group (0.106±0.052 and 0.073±0.049, respectively; p=0.005). Significantly lower sVEGFR-1 levels were determined in patients receiving metformin vs. without metformin using (0.065±0.016 and 0.118±0.053, respectively; p=0.001). Conclusion This is the first study evaluating and demonstrating the importance of plasma VEGF and sVEGFR-1 levels together in DM patients receiving insulin. Using metformin may have positive effect on angiogenesis in DM. Further studies are required to understand these effects.

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Wen-Dan Decoction Improves Negative Emotions in Sleep-Deprived Rats by Regulating Orexin-A and Leptin Expression

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/872547 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Fengzhi Wu ◽

Yuehan Song ◽

Feng Li ◽

Xin He ◽

Jie Ma ◽

...

Keyword(s):

Prefrontal Cortex ◽

Blood Serum ◽

Leptin Receptor ◽

Negative Emotions ◽

Field Tests ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Orexin A ◽

Therapeutic Mechanisms ◽

Leptin Expression

Wen-Dan Decoction (WDD), a formula of traditional Chinese medicine, has been clinically used for treating insomnia for approximately 800 years. However, the therapeutic mechanisms of WDD remain unclear. Orexin-A plays a key role in the sleep-wake cycle, while leptin function is opposite to orexin-A. Thus, orexin-A and leptin may be important factors in sleep disorders. In this study, 48 rats were divided into control, model, WDD-treated, and diazepam-treated groups. The model of insomnia was produced by sleep deprivation (SD) for 14 days. The expressions of orexin-A, leptin, and their receptors in blood serum, prefrontal cortex, and hypothalamus were detected by enzyme-linked immunosorbent assay, immunohistochemistry, and real time PCR. Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times. Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus. WDD treatment normalized the behavior and upregulated orexin-A, leptin, orexin receptor 1 and leptin receptor in brain. The findings suggest that WDD treatment may regulate SD-induced negative emotions by regulating orexin-A and leptin expression.

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Role of acute phase proteins in diagnosis of uremic pancreatitis and destructive pancreatitis in patients receiving renal replacement therapy (programmed hemodialysis)

Perm Medical Journal ◽

10.17816/pmj385115-122 ◽

2021 ◽

Vol 38 (5) ◽

pp. 115-122

Author(s):

Kazim G. Gasanov ◽

Viktor A. Zurnadzhyants ◽

Eldar A. Kchibekov ◽

M. I. Shikhragimov

Keyword(s):

Renal Replacement Therapy ◽

Serum Concentration ◽

Blood Serum ◽

Replacement Therapy ◽

Acute Phase Proteins ◽

Control Group ◽

Serum Concentrations ◽

High Concentration ◽

Surgical Unit

Objective. To determine the blood serum 2-microglobulin and 2-macroglobulin concentration in patients undergoing renal replacement therapy (programmed hemodialysis) for the diagnosis of uremic pancreatitis and / or destructive pancreatitis. Materials and methods. The study involved 52 patients admitted to the Surgical Unit of Astrakhan "RZhD-Medicine" Hospital and City Clinical Hospital № 3. The blood serum 2-microglobulin and 2-macroglobulin concentration was analyzed in patients admitted on an emergency basis with suspicion of uremic pancreatitis and destructive pancreatitis, who receive renal replacement therapy (programmed hemodialysis). The control group included 50 outpatients undergoing renal replacement therapy (programmed hemodialysis). The study did not include patients with suspected pancreatitis who were not receiving renal replacement therapy. The period of the study is 20192021. Results. The concentration of blood serum 2-microglobulin is statistically higher than normal in all patients, who had received renal replacement therapy (programmed hemodialysis) in anamnesis. The most statistically high concentration of 2-microglobulin was revealed while studying patients with uremic pancreatitis (n = 34), and was (30.0 2.75 mg/l) compared with the blood serum concentration in patients with destructive pancreatitis (8 0.51 mg / l). The concentration of 2-macroglobulin was statistically lower in destructive pancreatitis (n = 18) and was 615 161 mg/l compared with uremic pancreatitis (980 216 mg/l). In the control group of outpatients (n = 50) receiving renal replacement therapy (programmed hemodialysis), no statistically significant blood serum concentrations of 2-microglobulin and 2-macroglobulin were found. Conclusions. A clear dependence of the concentration of 2-microglobulin and 2-macroglobulin on the severity of uremic pancreatitis and destructive pancreatitis was established. Statistically high values of 2-microglobulin concentrations were obtained in patients with uremic pancreatitis, and the 2-macroglobulin level was statistically low in destructive pancreatitis.

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The role of myokines in the development of insulin resistance in children, with type 1 diabetes mellitus

Ukrainian Journal of Pediatric Endocrinology ◽

10.30978/ujpe2021-2-19 ◽

2021 ◽

pp. 19-26

Author(s):

O. Ye. Pashkova ◽

N. I. Chudova ◽

O. S. Litvinenko

Keyword(s):

Diabetes Mellitus ◽

Insulin Resistance ◽

Type 1 Diabetes ◽

Muscle Strength ◽

Blood Serum ◽

Muscle Mass ◽

Pediatric Patients ◽

Control Group

The aim — to study the role of myokines in the development of insulin resistance in children with type 1 diabetes mellitus.Materials and methods. Observations involved 68 children with type 1 diabetes mellitus (DM 1), with the mean age 11 to 17 years. Depending on the glycemic controllevel, patients were divided into 3 research groups. The control group consisted of 20 relatively healthy children. Muscle mass, the skeletal muscles index, fat mass and the percentage of fat in the bodywere determined in all patients. The Lovett’s test was used to assess the loss of muscle strength; evaluation of insulin resistance was made based onthe triglycerideglucose index (TYG). Levels of myostatin, irisin, interleukins 6 and 13were measured in blood serum.Results and discussion. It has been established that with deterioration in the level of glycemic controlin DM 1 children, the component redistribution of body composition took place with an increased fat mass proportionand decreased muscle mass. This resulted in the reduced insulin-mediatedabsorption of glucose, that was confirmed by the significant increase in TYG level compared to control group. The analysis of cytokines in the blood serum showed a significant increase in the level of myostatin and interleukin6 compared with the control group and the tendency to increased levels of the interleukins 13 and the level of irisin in the blood serum in pediatric patients with DM 1. The increased levels of myostatin in DM 1childrenassociated with an increase in the triglycerides content (r = 0.44, p < 0.05) and raised TYG index (r = 0.33, p < 0.05), testifying theclose correlation between the high myostatin levels and the development of insulin resistance.Conclusions. In children with diabetes mellitus, the reduction of muscle strength and muscle mass take place with a deterioration in the state of glycemic control, accompanying by the development of insulin resistance. The violation of myokines synthesis,along with the chronic hyperglycemia and diabetic myopathy, plays the leading role in the formation of insulin resistance in pediatric patients with DM 1. It is manifested by the increased production of myostatin and interleukin6 in the absence of activation of irisin and interleukin13synthesis.

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Viral and cellular cytokines in AIDS-related malignant lymphomatous effusions

Blood ◽

10.1182/blood.v96.4.1599 ◽

2000 ◽

Vol 96 (4) ◽

pp. 1599-1601 ◽

Cited By ~ 69

Author(s):

Yoshiyasu Aoki ◽

Robert Yarchoan ◽

James Braun ◽

Aikichi Iwamoto ◽

Giovanna Tosato

Keyword(s):

Vascular Endothelial Growth Factor ◽

Human Immunodeficiency Virus ◽

Potential Role ◽

Kaposi Sarcoma ◽

Enzyme Linked Immunosorbent Assay ◽

Vascular Endothelial ◽

Immunodeficiency Virus ◽

Endothelial Growth Factor ◽

Primary Effusion Lymphomas

Abstract Kaposi sarcoma-associated herpesvirus encodes viral IL-6 (vIL-6). To investigate the potential role of vIL-6 in the pathogenesis of human immunodeficiency virus (HIV)- related primary effusion lymphomas (PEL), a sensitive enzyme-linked immunosorbent assay was developed for vIL-6 and applied to the study of PEL. Whereas vIL-6 was detectable in 6 of 8 PEL effusions (range, 1390-66 630 pg/mL), it was not detectable in any of the control effusions. As expected, all PEL effusions contained human IL-6 (range, 957-37 494 pg/mL), and 7 of 8 contained detectable human IL-10 (range, 66-2,521,297 pg/mL). Human and vIL-6 have previously been shown to induce vascular endothelial growth factor, which in turn can increase vascular permeability. The results of the current study suggest that these cytokines play a central role in the pathogenesis and manifestations of PEL.

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A study of vascular endothelial growth factor in the cord blood of pre-eclamptics and healthy pregnant women

Asian Journal of Medical Sciences ◽

10.3126/ajms.v8i1.15711 ◽

2017 ◽

Vol 8 (1) ◽

pp. 21-25

Author(s):

Anita Rawat ◽

Anil Kumar Gangwar ◽

Archana Ghildiyal ◽

Neena Srivastava ◽

Sunita Tiwari ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Cord Blood ◽

Pregnant Women ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Vascular Endothelial ◽

Cord Serum ◽

Endothelial Growth Factor

Background: Pre-eclampsia(PE) is the most frequently encountered medical complication during pregnancy. In developing countries PE is a principal cause of maternal mortality. A disturbance in the angiogenic/antiangiogenic factors and in the hypoxia/placental re-oxygenation process, seems to activate a maternal endothelial dysfunction.Aims and Objective: To estimate Vascular Endothelial Growth Factor ( VEGF ) level in the cord blood of healthy and Preeclamptic ( PEc ) pregnant women and to associate this with Preeclamptic pregnancy.Material and Methods: A case-control study ofUmbilical cord serum VEGF levels from women with uncomplicated pregnancies (control group, n=60) and pregnancies complicated by Pre-eclampsia (n=40). VEGF in the cord serum was estimated by SANDWICH Enzyme Linked Immunosorbent Assay method by using ELISA Kit and then compared between the two groups.Results: The mean VEGF concentrations in the women who had pre-eclampsia (578.62±468.3) were lower than in the control group( 625.75±533.1) , but the difference was not statistically significant ( p= 0.8548). Conclusion VEGF plays a key role in the instability between endothelial dysfunction and angiogenesis that occurs during Preeclampsia. VEGF levels might be a useful tool for the early diagnosis of Pre-eclampsia.Asian Journal of Medical Sciences Vol.8(1) 2017 21-25

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Nesfatin-1 and Vaspin as Potential Novel Biomarkers for the Prediction and Early Diagnosis of Gestational Diabetes Mellitus

International Journal of Molecular Sciences ◽

10.3390/ijms20010159 ◽

2019 ◽

Vol 20 (1) ◽

pp. 159 ◽

Cited By ~ 11

Author(s):

Radzisław Mierzyński ◽

Elżbieta Poniedziałek-Czajkowska ◽

Dominik Dłuski ◽

Jolanta Patro-Małysza ◽

Żaneta Kimber-Trojnar ◽

...

Keyword(s):

Diabetes Mellitus ◽

Early Diagnosis ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Medical Complication ◽

Uncomplicated Pregnancy ◽

Novel Biomarkers

Gestational diabetes mellitus (GDM) is considered to be one of the most frequent medical complication observed among pregnant women. The role of adipokines in the pathogenesis of GDM remains strictly unknown. Different adipokines have been studied throughout gestation, and they have been proposed as biomarkers of GDM and other pregnancy-related complications; however, there is no biomarker reported for GDM screening at present. The aim of this study was to evaluate serum nesfatin-1 and vaspin levels in GDM and non-GDM women, to characterize the correlation between these adipokines, and to assess the potential role of circulating adipokines in the prediction of risk of gestational diabetes mellitus. Serum concentrations of nesfatin-1 and vaspin were measured in 153 women with GDM, and in 84 patients with uncomplicated pregnancy by enzyme-linked immunosorbent assay (ELISA) kits, according to the manufacturer’s instructions. Circulating levels of nesfatin-1 and vaspin were significantly lower in the GDM group than in the control group. Nesfatin-1 levels were negatively correlated with vaspin levels. The results of this study point out the possible role of nesfatin-1 and vaspin as potential novel biomarkers for the prediction and early diagnosis of GDM. Further studies are necessary to evaluate the influence of nesfatin-1 and vaspin on glucose metabolism in the early stages of GDM.

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Evaluation of Parvovirus B19 and Herpesvirus-6 Serology in Brazilian Children with Common B Cell Precursor Acute Lymphoblastic Leukemia

Blood ◽

10.1182/blood.v112.11.3944.3944 ◽

2008 ◽

Vol 112 (11) ◽

pp. 3944-3944

Author(s):

Fernanda A Silva ◽

Gisele M Vasconcelos ◽

Synara NS Cordeiro ◽

Mariana Sant’Ana ◽

Silvia Maia Farias de Carvalho ◽

...

Keyword(s):

B Cell ◽

Parvovirus B19 ◽

Lymphoblastic Leukemia ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Diagnostic Purpose ◽

Cell Precursor ◽

Blood Samples ◽

Brazilian Children

Abstract Background. Indirect epidemiologic evidences have suggested that viral infections may represent an important risk factor in the etiological mechanisms of B cell precursor lymphoblastic leukemia (BpALL), specially common-ALL. Parvovirus B19 (PvB19) is associated with aplastic crisis, thrombocytopenia, and congenital anemia. Five studies have reported the concurrent PvB19 infection in pediatric ALL. In a view of the increasing contest about the direct or indirect role of viral infection in ALL, we evaluated the possible associations of PvB19 and herpes virus 6 (HHV-6) in samples from children suffering from hematological disorders in order to estimate the magnitude of risk of association with common-BpALL. Material and Methods. A total of 659 blood samples were randomly selected to perform the serological tests. They were part of diagnostic samples referred to CPq-INCA-Rio de Janeiro, Brazil, from 2002–2006, for diagnostic purpose. Blood samples were exclusively selected from patients in whom samples were taken at the time of the onset of sickness. The diagnosis of BpALL was based on immunophenotyping methods according to EGIL criteria. The presence of serum antibodies for PvB19 and HHV-6 were determinate by enzyme-linked immunosorbent assay. Statistical analyses were conducted using SPSS for Windows 11.0. All p values were two sided; p<0.05 was considered statistically significant. Results. Common-ALL (n=176) and non-leukemic samples (n=107) considered as control group were stratified according to age-groups at diagnosis. Age distribution was: less than 2 years (15.5%); 2–6 years-old (46.0%), and 7–12 years-old (17.7%). The estimation of associated risk PvB19 IgG+ antibodies with common-BpALL was OR 0.53 95% CI (0.52–0.54); whereas HHV-6 IgG was OR 2.36, 95% CI (1.08–5.17). Conclusions. We find out a higher prevalence of HHV-6 antibodies in patients with common-BpALL, suggesting an association between HHV-6 infection e common-BpALL in Brazilian children. There is a long history in developed countries regarding the role of infections in leukemogenesis process. Further tests are necessary to confirm these results.

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