scholarly journals Wireless Pressure Sensor Assisted Orthopedic Nursing Effectiveness Evaluation

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Qiufang Wang ◽  
Yumei Shen
Keyword(s):  
Real Time ◽  
Pressure Sensor ◽  
Bone Matrix ◽  
Noninvasive Monitoring ◽  
Peak Time ◽  
Regression Equations ◽  
Stress Difference ◽  
Sensor Unit ◽  
Osteoblast Activity ◽  
Upper Computer

This paper combines flexible pressure sensing technology, wireless sensor network, and cloud platform technology to design and manufacture a medical miniature pressure sensor and its supporting system. The problem of noninvasive monitoring of the syndrome encountered in the clinic is used for real-time monitoring and auxiliary diagnosis of the disease. Different from the current clinical use of “puncture” to measure intrafascial pressure, this system focuses on the noninvasive monitoring of compartment syndrome, using medical tape to paste a flexible microsensing unit on the injured area. The flexible sensor unit can measure the pressure here in real time and then can know the pressure in the fascia chamber. The flexible pressure sensor unit combines with the subsequent flexible circuit to send the measured data to the data in real time through wireless communication. The data aggregation node transmits the collected data to the upper computer through serial communication, and the upper computer software processes and stores the data and uploads it to the cloud server. In this experiment, it was observed that the concentrations of Ca and P showed the same fluctuating trend. With the gradual progress of the stretch, the concentrations of Ca and P increased with the increase in time, reaching approximately at the end of the extension. The peak value indicates that the osteoclast activity is enhanced at this time, the bone matrix is largely destroyed, and the Ca and P in the matrix are released into the serum in a large amount, thereby increasing the serum concentration. After the distraction ceases, it enters the healing period of the callus. At this time, the concentrations of Ca and P decrease with the increase in time and gradually reach a stable level, indicating that the osteoblast activity is enhanced at this time, the bone matrix begins to rebuild, and the Ca and P gradually increase. The deposited bone matrix gradually forms new bone and finally reaches a balance. Since the speed of extension in each experimental group is inconsistent, the time required to reach the same extension length is also inconsistent, so that the peak time is also inconsistent. After plotting the stress difference ( △ F ) before and after stretching against time and speed, it is found that the relationship is linear. However, these two variables affect △ F at the same time, so they cannot be isolated. Based on this, this subject uses multiple regression equations to fit the three relationships of stress difference ( △ F ), time, and speed. In the process of distraction osteogenesis, with each distraction, the bone stress presents a trend from high to low. And as the stretch progresses, the measured stress value increases linearly at the same time point every day.

Osteoblast iron genes: real time PCR and microarray hybridization approach under hyperoxia

2021 ◽  
Vol 32 (4) ◽  
pp. 491-496
Author(s):  
Prihartini Widiyanti ◽  
Hartmut Kuehn ◽  
Soetjipto Soetjipto
Keyword(s):  
Real Time ◽  
Transferrin Receptor ◽  
Bone Growth ◽  
Receptor Gene ◽  
Bone Matrix ◽  
Expression Level ◽  
Receptor Mrna ◽  
Osteoblast Activity ◽  
Gapdh Mrna ◽  
Iron Regulated Transporter

Abstract Objectives Iron is essential for cell growth, differentiation, electron transfer, and oxygen transport. Hyperoxia may increase the turnover of bone matrix components with a net effect of accelerated bone growth. Although hyperoxia was claimed could increase osteoblast activity, but expression level in possible genes which play role in proliferation is still unclear. This research aims to prove the differences of expression level of transferrin receptor gene and iron regulated transporter and other genes of 7F2 under 24 h normoxia, 24 h hyperoxia, and 48 h hyperoxia and the effect of hyperoxia by using osteoblast cell culture 7F2. Methods Reverse transcriptase, real time Polymerase Chain Reaction (PCR), and microarray is used to qualitatively detect gene expression. The computer softwares such as National Center for Biotechnology Information (NCBI) data base, Software Affymetrix, DNA Strider program, Genomatix – DiAlign program, Oligo 5.0 program (Software primer design) from Wojciech & Piotr Rychlik, and Genetyx-Mac version 8.0 have been used to analyze the PCR result. Results Under 24 h hyperoxia, there were 3,884 copies of transferrin receptor mRNA per 1,000,000 copies of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA. After 24 h hyperoxia, 8,325 copies of transferrin receptor mRNA per 1,000,000 GAPDH mRNA copies were found showing 2.1-fold up regulation. After 48 h hyperoxia, there was no significant increase at the level of expression of transferrin receptor mRNA, 8,079 mRNA copies per 1,000,000 copies of mRNA were found (2.0-fold up regulation compared with 24 h normoxia). Conclusions It can be concluded that hyperoxia might have an effect on upregulating the expression of some osteoblast genes which might have an impact on osteoblast activity.

A Wireless Power-Free Pressure Sensor for Real-Time In Vivo Gastrointestinal Pressure Monitoring

2014 ◽  
Vol 609-610 ◽  
pp. 993-996
Author(s):  
Qiang Shi ◽  
De Yong Chen ◽  
Jun Bo Wang ◽  
Kai Kai Bao ◽  
Li Juan Liu
Keyword(s):  
Real Time ◽  
Pressure Sensor ◽  
Low Cost ◽  
Wireless Power ◽  
Pressure Monitoring ◽  
Device Structure ◽  
Detection Mechanism ◽  
Sensor Unit ◽  
Detection Unit

A wireless and power-free pressure sensor system capable of real time in vivo gastrointestinal pressure monitoring has been developed. This system contains a sensor unit and a detection unit. Based on mutual inductance detection mechanism, the sensor is featured with simple device structure and therefore low cost. The packaged sensor unit was tested. Results obtained from experiment demonstrated that this sensor has a sensitivity of 0.2115 kHz / kPa within a pressure range-10~30 kPa. The in vivo testing result not only indicates a period of 2 contractions per minute peristalsis of rabbit stomach but also validates the feasibility of this real time wireless gastrointestinal pressure monitoring system.

Galactocerebrosides, Essential for Hematopoietic Progenitor Mobilization, Regulate SDF-1 (CXCL12)-Mediated Attraction to Bone.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 665-665 ◽  
Author(s):  
Yoshio Katayama ◽  
Andres Hidalgo ◽  
Paul S. Frenette
Keyword(s):  
Bone Marrow ◽  
Real Time ◽  
Matrix Protein ◽  
Bone Matrix ◽  
Extracellular Fluid ◽  
Hematopoietic Progenitor Cell ◽  
Rt Pcr ◽  
Hematopoietic Progenitor ◽  
Osteoblastic Activity ◽  
Osteoblast Activity

Abstract The exact mechanisms mediating G-CSF-induced hematopoietic progenitor cell (HPC) egress from the bone marrow (BM) are incompletely understood. Recent studies have suggested that the degradation of SDF-1 in the BM by G-CSF-induced proteolysis may play an important role. We previously hypothesized that endogenous galactocerebrosides (GCs) might be involved in HPC trafficking since certain sulfogalactolipids share biological properties with fucoidan, a sulfated fucose polymer endowed with mobilization activity, and showed that G-CSF fails to induce HPC mobilization in mice lacking UDP-galactose:ceramide galactosyl transferase (CGT) (Blood 2001 98:811a), the enzyme necessary for GC synthesis. To gain further mechanistic insights, we assessed protease activity and found no difference in elastase release from BM cells and in the degradation of exogenous SDF-1 in BM extracellular fluid (BMEF) between CGT−/− and +/+ littermates. Furthermore, endogenous SDF-1 levels in BMEF of CGT−/− and +/+ mice showed a similar reduction after G-CSF stimulation (>50% in CGT−/− mice, n=7–9, p<0.05) despite a virtual absence of mobilization. These data suggest that the reduction of SDF-1 in bone marrow is not essential for G-CSF-induced mobilization. To evaluate the spacial distribution of SDF-1 expression in mouse BM, we stained SDF-1 using the tyramide amplification system. We found that SDF-1 staining was sparsely distributed in the BM but, surprisingly, strong homogenous staining was observed in the surrounding bone. Staining specificity was confirmed by ELISA (2.6±0.5 vs 5.8±1.0 ng SDF-1 per femur for BMEF and bone protein extracts, respectively, n=8, p<0.05). Following G-CSF stimulation, SDF-1 protein levels were significantly decreased in bone extracts from CGT+/+ littermates (53% reduction, n=4–5, p<0.05), but were virtually unchanged in CGT−/− mice. Quantitative real-time RT-PCR analyses revealed that SDF-1 was transcriptionally downregulated by G-CSF in both BM and bone in CGT+/+ mice but there was no significant reduction in CGT−/− bone. Since osteoblasts may represent a major source of SDF-1, we suspected that osteoblast activity might be altered in CGT−/− mice. We thus measured plasma osteocalcin levels by ELISA and found a significant reduction in CGT−/− mice compared to CGT+/+ littermates (39% reduction, n=6–9, p<0.001). Immunofluorescence experiments revealed that bone lining osteoblasts in CGT−/− mice were flattened and small while in CGT+/+ littermates, these cells displayed a healthy cobblestone-like appearance. Furthermore, there was a trend toward reduction of gene expression in Runx2, a critical transcription factor in osteoblasts, and α1(I) collagen, an osteoblast-specific bone matrix protein, in CGT−/− BM by real-time RT-PCR. These data suggest that dysregulation of bone SDF-1 in CGT−/− mice may be due to constitutive downregulation of osteoblastic activity. Strikingly, Runx2 and α1(I) collagen were dramatically downregulated by G-CSF in CGT+/+ BM (Runx2; 65% reduction, n=4, p<0.001, α1(I) collagen; 92% reduction, n=4, p<0.05 by real-time RT-PCR). G-CSF does not appear to act directly on osteoblasts since G-CSF receptor mRNA was not detectable in primary osteoblast and 4 different osteoblast lineage cell lines. In conclusion, bone SDF-1, rather than that of BM, may regulate HPC mobilization. The abnormal regulation of bone SDF-1 and reduced osteoblastic activity in CGT−/− mice strongly suggest that bone SDF-1 originates from osteoblasts and that a rapid downregulation of osteoblastic activity may play a key role in the egress of stem cells from BM.

Noninvasive Monitoring of Arterial Viscoelastic Indices Using a Foil-type Pressure Sensor

2011 ◽  
Vol 131 (9) ◽  
pp. 1518-1527
Author(s):  
Hiromi Maruyama ◽  
Harutoyo Hirano ◽  
Abdugheni Kutluk ◽  
Toshio Tsuji ◽  
Osamu Fukuda ◽  
...  
Keyword(s):  
Pressure Sensor ◽  
Noninvasive Monitoring ◽  
Type Pressure

A Real Time Vehicle Collision Sensor and Reporting System Using Load Sensor, GSM and GPS Technology

2013 ◽  
Vol 718-720 ◽  
pp. 1740-1745
Author(s):  
Tulu Muluneh Mekonnen ◽  
De Ning Jiang ◽  
Yong Xin Feng
Keyword(s):  
Real Time ◽  
Pressure Sensor ◽  
Reporting System ◽  
Electronic Device ◽  
Text Message ◽  
Sensor System ◽  
Gps Receiver ◽  
Gps Technology ◽  
Vehicle Collision ◽  
Load Sensor

Vehicle collision sensor system and reporting accident to police is an electronic device installed in a vehicle to inform police man in case of accident to track the vehicles location. This system works using pressure sensor, GPS and GSM technology. These technology embedded together to sense the vehicle collision and indicate the position of the vehicle or locate the place of accident in order to solve the problem immediately (as soon as possible).For doing so AT89S52 microcontroller is interfaced serially to a GSM modem, GPS receiver, and pressure sensor. A GSM modem is used to send the position (Latitude and Longitude) of the vehicle, the plate of the vehicle and the SMS text from the accident place. The GPS modem will continuously give the data (longitude and latitude) and Load sensor senses the collision of the vehicle against obstacles and input to microcontroller. As load sensor senses the collision, the GSM start to send the plate of the vehicle, text message and the position of the vehicle in terms of latitude and longitude in real time.

scholarly journals Osteoblastic Response to the Defective Matrix in the Osteogenesis Imperfecta Murine (oim) Mouse

Endocrinology ◽  
2002 ◽  
Vol 143 (5) ◽  
pp. 1594-1601 ◽  
Author(s):  
I. Kalajzic ◽  
J. Terzic ◽  
Z. Rumboldt ◽  
K. Mack ◽  
A. Naprta ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Osteogenesis Imperfecta ◽  
Bone Matrix ◽  
High Rate ◽  
Transferase Activity ◽  
Osteoblast Activity ◽  
Chloramphenicol Acetyl Transferase Activity ◽  
High Bone ◽  
Normal Quantity ◽  
And Control

Abstract This work examines the cellular pathophysiology associated with the weakened bone matrix found in a murine model of osteogenesis imperfecta murine (oim). Histomorphometric analysis of oim/oim bone showed significantly diminished bone mass, and the osteoblast and osteoclast histomorphometric parameters were increased in the oim/oim mice, compared with wild-type (+/+) mice. To assess osteoblast activity, a rat Col1a1 promoter linked to the chloramphenicol acetyltransferase reporter transgene was bred into the oim model. At 8 d and 1 month of age, no difference in transgene activity between oim and control mice was observed. However, at 3 months of age, chloramphenicol acetyl transferase activity was elevated in oim/oim;Tg/Tg, compared with +/+;Tg/Tg and oim/+;Tg/Tg. High levels of urinary pyridinoline crosslinks in the oim/oim;Tg/Tg mice were present at all ages, reflecting continuing high bone resorption. Our data portray a state of ineffective osteogenesis in which the mutant mouse never accumulates a normal quantity of bone matrix. However, it is only after the completion of the rapid growth phase that the high activity of the oim/oim osteoblast can compensate for the high rate of bone resorption. This relationship between bone formation and resorption may explain why the severity of osteogenesis imperfecta decreases after puberty is completed. The ability to quantify high bone turnover and advantages of using a transgene that reflects osteoblast lineage activity make this a useful model for studying interventions designed to improve the bone strength in osteogenesis imperfecta.

IGF-binding protein-5 stimulates osteoblast activity and bone accretion in ovariectomized mice

2001 ◽  
Vol 281 (2) ◽  
pp. E283-E288 ◽  
Author(s):  
Dennis L. Andress
Keyword(s):  
Bone Formation ◽  
Binding Protein ◽  
Formation Rate ◽  
Bone Matrix ◽  
Secretory Protein ◽  
Mineral Density ◽  
Bone Formation Rate ◽  
Ovariectomized Mice ◽  
Osteoblast Activity

Insulin-like growth factor binding protein-5 (IGFBP-5) is an osteoblast secretory protein that becomes incorporated into the mineralized bone matrix. In osteoblast cultures, IGFBP-5 stimulates cell proliferation by an IGF-independent mechanism. To evaluate whether IGFBP-5 can stimulate osteoblast activity and enhance bone accretion in a mouse model of osteoblast insufficiency, daily subcutaneous injections of either intact [IGFBP-5 (intact)] or carboxy-truncated IGFBP-5 [IGFBP-5-(1–169)] were given to ovariectomized (OVX) mice for 8 wk. Femur and spine bone mineral density (BMD), measured every 2 wk, showed early and sustained increases in response to IGFBP-5. Bone histomorphometry of cancellous bone showed significant elevations in the bone formation rate in both the femur metaphysis [IGFBP-5- (1)] only) and spine compared with OVX controls. IGFBP-5 also stimulated osteoblast number in the femur IGFBP-5-(1–169) only) and spine. These data indicate that IGFBP-5 effectively enhances bone formation and bone accretion in OVX mice by stimulating osteoblast activity. The finding that IGFBP-5-(1–169) is bioactive in vivo indicates that the carboxy-terminal portion is not required for this bone anabolic effect.

Realization of Miniature Air Data System Based on Silicon Micro Pressure Sensor

2012 ◽  
Vol 503-504 ◽  
pp. 1445-1449
Author(s):  
Li Min Chang ◽  
Xiang Bin Yu ◽  
Li Jing Zhang
Keyword(s):  
Mach Number ◽  
High Resolution ◽  
Real Time ◽  
Pressure Sensor ◽  
Pressure Measurement ◽  
High Stability ◽  
Computer Calculation ◽  
High Accuracy ◽  
Data System ◽  
Embedded Computer

In this paper, miniature air data system is designed based on thermally excited resonant silicon micro structural pressure sensor. The system employs thermally excited resonant silicon micro structural pressure sensor for the pressure measurement. Using miniature embedded computer, calculation of the parameters such as height, airspeed and mach number and real-time display by LCD are realized. The volume and weight of this system is only one-twelfth of the original. In addition, it has the characteristics of high accuracy, high resolution, high stability and repeatability.

Design of Electromagnetic Smart Car System

2011 ◽  
Vol 130-134 ◽  
pp. 343-346
Author(s):  
Meng Jun Ye ◽  
Chang Hui Hu
Keyword(s):  
Real Time ◽  
Control Unit ◽  
The Self ◽  
Position Sensor ◽  
Sensor Unit ◽  
The Core ◽  
Electromagnetic Sensor ◽  
Forward Looking

The document introduces the self-tracing smart car system based on Fressscale MC9S12XS128 as the core control unit. Focus on the design of electromagnetic sensor unit, describe in detail differential electromagnetic sensor unit and position electromagnetic sensor unit, and then describe the speed adjusting system. Test shows that smart car based on position sensor unit have good forward-looking, real-time and accurate response.

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