Neuronal Differentiation Modulated by Polymeric Membrane Properties

2017 ◽  
Vol 204 (3-4) ◽  
pp. 164-178 ◽  
Author(s):  
Sabrina Morelli ◽  
Antonella Piscioneri ◽  
Enrico Drioli ◽  
Loredana De Bartolo
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Neuronal Differentiation ◽  
Membrane Properties ◽  
Polymeric Membrane ◽  
Neuronal Growth ◽  
Neuronal Structure ◽  
Elongation At Break ◽  
Neuronal Tissue ◽  
Potential Use

In this study, different collagen-blend membranes were successfully constructed by blending collagen with chitosan (CHT) or poly(lactic-co-glycolic acid) (PLGA) to enhance their properties and thus create new biofunctional materials with great potential use for neuronal tissue engineering and regeneration. Collagen blending strongly affected membrane properties in the following ways: (i) it improved the surface hydrophilicity of both pure CHT and PLGA membranes, (ii) it reduced the stiffness of CHT membranes, but (iii) it did not modify the good mechanical properties of PLGA membranes. Then, we investigated the effect of the different collagen concentrations on the neuronal behavior of the membranes developed. Morphological observations, immunocytochemistry, and morphometric measures demonstrated that the membranes developed, especially CHT/Col30, PLGA, and PLGA/Col1, provided suitable microenvironments for neuronal growth owing to their enhanced properties. The most consistent neuronal differentiation was obtained in neurons cultured on PLGA-based membranes, where a well-developed neuronal network was achieved due to their improved mechanical properties. Our findings suggest that tensile strength and elongation at break are key material parameters that have potential influence on both axonal elongation and neuronal structure and organization, which are of fundamental importance for the maintenance of efficient neuronal growth. Hence, our study has provided new insights regarding the effects of membrane mechanical properties on neuronal behavior, and thus it may help to design and improve novel instructive biomaterials for neuronal tissue engineering.

scholarly journals Development of Photocrosslinkable Urethane-Doped Polyester Elastomers for Soft Tissue Engineering

2011 ◽  
Vol 1 (1) ◽  
pp. 18-31 ◽  
Author(s):  
Yi Zhang ◽  
Richard T. Tran ◽  
Dipendra Gyawali ◽  
Jian Yang
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Soft Tissue ◽  
Hexamethylene Diisocyanate ◽  
Elongation At Break ◽  
Thermally Induced ◽  
Molar Ratios ◽  
3D Network ◽  
Soft Tissue Engineering

Finding an ideal biomaterial with the proper mechanical properties and biocompatibility has been of intense focus in the field of soft tissue engineering. This paper reports on the synthesis and characterization of a novel crosslinked urethane-doped polyester elastomer (CUPOMC), which was synthesized by reacting a previously developed photocrosslinkable poly (octamethylene maleate citrate) (POMC) prepolymers (pre-POMC) with 1,6-hexamethylene diisocyanate (HDI) followed by thermo- or photo-crosslinking polymerization. The mechanical properties of the CUPOMCs can be tuned by controlling the molar ratios of pre-POMC monomers, and the ratio between the prepolymer and HDI. CUPOMCs can be crosslinked into a 3D network through polycondensation or free radical polymerization reactions. The tensile strength and elongation at break of CUPOMC synthesized under the known conditions range from 0.73±0.12MPa to 10.91±0.64MPa and from 72.91±9.09% to 300.41±21.99% respectively. Preliminary biocompatibility tests demonstrated that CUPOMCs support cell adhesion and proliferation. Unlike the pre-polymers of other crosslinked elastomers, CUPOMC pre-polymers possess great processability demonstrated by scaffold fabrication via a thermally induced phase separation method. The dual crosslinking methods for CUPOMC pre-polymers should enhance the versatile processability of the CUPOMC used in various conditions. Development of CUPOMC should expand the choices of available biodegradable elastomers for various biomedical applications such as soft tissue engineering.

Preparation and Characterization of Poly(Vinyl Alcohol)(PVA)/Hydroxyapatite (HA) Nanofibrous Scaffolds

2011 ◽  
Vol 284-286 ◽  
pp. 459-463 ◽  
Author(s):  
Yuan Yuan Qi ◽  
Bin Liu ◽  
Xing Bin Yan
Keyword(s):  
Mechanical Properties ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Tensile Strength ◽  
Drug Delivery Systems ◽  
Poly Vinyl Alcohol ◽  
Elongation At Break ◽  
Nanofibrous Scaffolds ◽  
Potential Applications

Nanofibrous scaffolds of PVA and HA were prepared by electrospinning. SEM showed the scaffolds had porous nanofibrous morphology, and the diameter of the fibers was in the range of 200-1000 nm. FTIR and XRD showed the presence of HA in the scaffolds. The mechanical properties of the scaffolds changed by the adding content of HA. For the nanoscaffolds with 2wt % HA, the ultimate tensile strength and the elongation at break was 7.5 MPa and 17%. The PVA/HA nanoscaffolds prepared by electrospinning indicated good properties, and had a potential applications in bone tissue engineering and drug delivery systems.

Preparation and properties of poly(ε-caprolactone)/bioactive glass nanofibre membranes for skin tissue engineering

2017 ◽  
Vol 33 (2) ◽  
pp. 195-209 ◽  
Author(s):  
Zefeng Lin ◽  
Wendong Gao ◽  
Limin Ma ◽  
Hong Xia ◽  
Weihan Xie ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bioactive Glass ◽  
Fibre Diameter ◽  
Skin Tissue ◽  
Glass Mass ◽  
Glass Composite ◽  
Elongation At Break ◽  
Skin Tissue Engineering ◽  
Electrospun Nanofibres

Poly(ε-caprolactone) composite nanofibres for skin tissue engineering and regeneration applications were prepared via electrospinning of poly(ε-caprolactone) nanofibres with bioactive glass nanoparticles at bioactive glass contents of 0, 2, 4, 6 and 8 wt%. The surface properties, water absorptivities, porosities, mechanical properties and biocompatibilities of the composite electrospun nanofibres were characterised in detail. Addition of bioactive glass improved the hydrophilicity and elastic modulus of membranes. The fibre diameter of the neat poly(ε-caprolactone) nanofibres was only 700 nm, but reinforcement with 2, 4, 6 and 8 wt% bioactive glass nanofibres increased the diameter to 1000, 1100, 900 and 800 nm, respectively. The minimum elongation at break of the bioactive glass–reinforced poly(ε-caprolactone) exceeded 100%, which indicated that the composite nanofibres had good mechanical properties. The porosities of the various nanofibres containing different mass loadings of bioactive glass all exceeded 90%. The best performance in terms of cell proliferation and adhesion was found when the bioactive glass mass percent reached 6 wt%. However, higher loadings were unfavourable for cell growth. These preliminary results indicate that poly(ε-caprolactone)/bioactive glass composite nanofibres have promise for skin tissue engineering applications.

Controlled Release of Quercus infectoria Extract from Jackfruit Starch-Konjac Glucomannan Film

2014 ◽  
Vol 875-877 ◽  
pp. 187-190
Author(s):  
Chutimon Satirapipathkul ◽  
Katima Jantarapun
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Controlled Release ◽  
Konjac Glucomannan ◽  
Glycerol Content ◽  
Elongation At Break ◽  
Blend Film ◽  
Blend Films ◽  
Potential Use ◽  
Quercus Infectoria

Jackfruit (JS)-konjac glucomannan (KGM) blend films were prepared using different concentrations of JS, KGM and glycerol. Their mechanical properties were determined. All the films exhibited significantly higher values of tensile strength and elongation at break than those of neat JS films. The values varied considerably depending on KGM and glycerol content.Quercus infectoriaextract was embedded into the films and its release characteristics were controlled by changing the initial KGM content. This study suggests that the blend film has a potential use for advanced controlled release of bioactive extracts.

Neuronal Outgrowth and Differentiation on Poly(glycerol sebacate)

2012 ◽  
Vol 1417 ◽  
Author(s):  
Meghan E. Casey ◽  
Sabrina S. Jedlicka
Keyword(s):  
Mechanical Properties ◽  
Cell Differentiation ◽  
Neurite Outgrowth ◽  
Neuronal Differentiation ◽  
Matrix Effects ◽  
Neuronal Cell ◽  
Stem Cell Differentiation ◽  
Neuronal Tissue ◽  
Neuronal Cell Differentiation ◽  
The Impact

ABSTRACTThe importance of the extracellular mechanical environment in stem cell differentiation has been extensively studied over the last decade. In neuronal cell differentiation, matrix stiffness and neurite outgrowth have been correlated, highlighting the impact of matrix effects on neuronal cell morphology. In addition, on materials that approach the physiological mechanical properties of brain tissue, neurons from mixed phenotype primary cultures will prevail. However, if the same mixed culture is grown on polystyrene, glial populations are more prevalent. Enhancing the understanding of these differentiation processes will further expand the ability to design materials for neuronal implants that are conducive to neuronal survival, resist glial scarring and promote neurite outgrowth and cell connectivity. Specifically, elastomers such as poly(glycerol sebacate) (PGS) hold promise in neuronal tissue engineering, due to their mechanical tunability. PGS is biocompatible, biodegradable and possesses mechanical properties similar to that of living tissue. Neuronal cell differentiation was studied on PGS, using P19 embryonic carcinoma cells, which can be differentiated into a neuronal phenotype using retinoic acid. Varying cure temperatures of PGS including 120°, 140° and 165°C were selected, which equate to an elastic modulus of 0.07, 0.43 and 2.30 MPa respectively. Cells were characterized via immunocytochemistry. A primarily astrocytic population, with limited neuronal differentiation and neurite outgrowth were observed on the PGS 120°C. Cells grown on PGS 140°C demonstrated marked neurite outgrowth, with an increase in neuronal cells. Cells grown on the PGS 165°C exhibited the largest population of neurons, with significant neurite outgrowth. These results indicate that substrate mechanical properties do impact neuronal differentiation, but that a material with a Young’s modulus similar to that of neuronal tissue (PGS 120°C) may not necessarily be the most conducive to in vitro differentiation.

scholarly journals Effect of Polymer Concentration on the Morphology and Mechanical Properties of Asymmetric Polysulfone (PSf) Membrane

2017 ◽  
Vol 21 (1) ◽  
Author(s):  
N. M. Ismail ◽  
N. R. Jakariah ◽  
N. Bolong ◽  
S. M. Anissuzaman ◽  
N. A. H. M. Nordin ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Mechanical Strength ◽  
Polymer Concentration ◽  
Membrane Properties ◽  
Morphological Properties ◽  
Elongation At Break ◽  
Membrane Morphology ◽  
Tensile Tester ◽  
Morphology And Mechanical Properties

Polymer concentration had been identified as one of the important parameters tailoring the membrane properties. In this work, the effects of polymer concentration on the morphological and mechanical properties of membrane were investigated at three different polymer concentrations, i.e., 20, 25 and 30 wt%. The viscosity of dope solutions were determined to estimate the optimum polymer concentration.  The morphological properties of the fabricated membrane were determined using SEM whereas the mechanical properties of the membrane were investigated using tensile tester. Results show that an increase in the polymer concentration could lead to the improvement of the morphological and mechanical properties of the membrane. The tensile strength of the membrane determined for PSf-20, PSf-25 and PSf-30 are 5.73, 6.59 and 7.03 MPa, respectively whereas the elongation at break measured for the membranes are 46.99%, 69.18% and 36.27%, respectively. As shown in this work, the polymer concentration played a significant role to alter on membrane morphology and mechanical strength. 

Hydrogels as Antibacterial Biomaterials

2018 ◽  
Vol 24 (8) ◽  
pp. 843-854 ◽  
Author(s):  
Weiguo Xu ◽  
Shujun Dong ◽  
Yuping Han ◽  
Shuqiang Li ◽  
Yang Liu
Keyword(s):  
Mechanical Properties ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Water Content ◽  
Oxygen Permeability ◽  
Structural Diversity ◽  
High Water ◽  
Clinical Applications ◽  
High Water Content ◽  
Biomedical Field

Hydrogels, as a class of materials for tissue engineering and drug delivery, have high water content and solid-like mechanical properties. Currently, hydrogels with an antibacterial function are a research hotspot in biomedical field. Many advanced antibacterial hydrogels have been developed, each possessing unique qualities, namely high water swellability, high oxygen permeability, improved biocompatibility, ease of loading and releasing drugs and structural diversity. In this article, an overview is provided on the preparation and applications of various antibacterial hydrogels. Furthermore, the prospects in biomedical researches and clinical applications are predicted.

Biocompatiblibility analysis of the decellularized bovine pericardium

2020 ◽  
Vol 8 (2) ◽  
Author(s):  
A. Sokol ◽  
◽  
D. Grekov ◽  
G. Yemets ◽  
O. Galkin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sodium Dodecyl ◽  
Bovine Pericardium ◽  
Surrounding Tissue ◽  
Tissue Samples ◽  
Group I ◽  
Alternative Material ◽  
Decellularized Scaffolds ◽  
Group 2 ◽  
Potential Use

The decellularized bovine pericardium and its potential use as a natural scaffold is a promising approach in the field of tissue engineering and regenerative medicine. The reaction of the host toward decellularized scaffolds depends on their biocompatibility, which should be satisfied being before applied in clinical use. Purpose: to evaluate the biocompatibility of the extracellular matrices, which were decellularized by trypsin enzyme and anionic sodium dodecyl sulfate (SDS) detergent. Material and methods. Pericardial sacs were acquired from 12-18 months’ age bulls. Tissue decellularization was performed by using 0.25 % Trypsin solution and 1 % ionic SDS for group I and 0.1 % SDS for group II samples. The implantation was performed on Wistar rats. The tissue samples were stained with hematoxylin & eosin, Congo red and Masson's Trichrome for histological analysis. Results. In group 1 in 3 months after subcutaneous implantation in rats we noticed the inflammation in surrounding tissue and degradation of the implant. Under the same conditions in animals of group 2 implant replacement with growing immature connective tissue was noted. Bio-implant of this group did not degrade, moreover it's integrated to the tissues of experimental rats. Conclusion. Our results showed that decellularized bovine pericardium by 0.1 % SDS can become an alternative material for tissue engineering and has the potential for further use in human surgery.

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

scholarly journals Effect of Organic Modifier Types on the Physical–Mechanical Properties and Overall Migration of Post-Consumer Polypropylene/Clay Nanocomposites for Food Packaging

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1502
Author(s):  
Eliezer Velásquez ◽  
Sebastián Espinoza ◽  
Ximena Valenzuela ◽  
Luan Garrido ◽  
María José Galotto ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Food Packaging ◽  
Clay Nanocomposites ◽  
Organic Modifier ◽  
Thermal And Mechanical Properties ◽  
Chemical Safety ◽  
Elongation At Break ◽  
Fatty Food ◽  
Recycled Plastics ◽  
Thermal Stability Of

The deterioration of the physical–mechanical properties and loss of the chemical safety of plastics after consumption are topics of concern for food packaging applications. Incorporating nanoclays is an alternative to improve the performance of recycled plastics. However, properties and overall migration from polymer/clay nanocomposites to food require to be evaluated case-by-case. This work aimed to investigate the effect of organic modifier types of clays on the structural, thermal and mechanical properties and the overall migration of nanocomposites based on 50/50 virgin and recycled post-consumer polypropylene blend (VPP/RPP) and organoclays for food packaging applications. The clay with the most hydrophobic organic modifier caused higher thermal stability of the nanocomposites and greater intercalation of polypropylene between clay mineral layers but increased the overall migration to a fatty food simulant. This migration value was higher from the 50/50 VPP/RPP film than from VPP. Nonetheless, clays reduced the migration and even more when the clay had greater hydrophilicity because of lower interactions between the nanocomposite and the fatty simulant. Conversely, nanocomposites and VPP/RPP control films exhibited low migration values in the acid and non-acid food simulants. Regarding tensile parameters, elongation at break values of PP film significantly increased with RPP addition, but the incorporation of organoclays reduced its ductility to values closer to the VPP.

Sign in / Sign up

Export Citation Format

Share Document