Immobilization of polyoxometalate on aminosilane-modified silicate: Synthesis, characterization and antibacterial performance

2019 ◽  
Vol 9 (8) ◽  
pp. 970-977
Author(s):  
Kuo-Hui Wu ◽  
Yin-Chiung Chang ◽  
Je-Chuang Wang
Keyword(s):  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Sol Gel ◽  
Covalent Bond ◽  
Zone Of Inhibition ◽  
Antibacterial Effects ◽  
Antibacterial Performance ◽  
Acidic Conditions ◽  
Better Than

This paper presents studies on the immobilization of the polyoxometalate (H5PV2Mo10O40) on aminosilanemodified silicate as a new barrier of biological toxicant. The polyoxometalate-doped aminosilane-modified silicate (Ormosil/POM hybrid and Ormosil + POM blend) were synthesized by an in situ sol–gel route under acidic conditions. The physical properties of the samples were examined using NMR, SEM, XPS and TGA spectroscopy. The results indicated that POM was immobilized in channels by covalent linkages with the Ormosil of the Ormosil/POM hybrid, and that POM was incorporated into the Ormosil after impregnation from the Ormosil + POM blend. The covalent bond resulted from the reaction of Ormosil with POM, in which Si species were inserted into the vacancy of lacunary POM to form Si–O–Mo bonds. The antibacterial effects of samples were investigated with zone of inhibition testing, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The Ormosil/POM hybrid and Ormosil + POM blend possessed excellent antibacterial abilities and the antibacterial performance of the Ormosil/POM hybrid was better than that of the Ormosil + POM blend.

scholarly journals DIFFERENCE BETWEEN ANTIMICROBIAL EFFECTS OF PAPACARIE AND PAPAIN ON STREPTOCOCCUS MUTANS IN VITRO

2019 ◽  
pp. 79-83
Author(s):  
TITTY SULIANTI ◽  
NILAKESUMA DJAUHARI ◽  
BAMBANG NURSASONGKO
Keyword(s):  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Zone Of Inhibition ◽  
Antimicrobial Effects ◽  
Treatment Groups ◽  
Group Control ◽  
Caries Removal ◽  
And Diffusion ◽  
Better Than

Objective: The aim is to compare the antimicrobial effects of papain and Papacarie with dilution and diffusion tests.Methods: There were two treatment groups and one Group control. The treatment group received papain and Papacarie, and the control groupreceived chlorhexidine, in five liquids with different concentrations of 0.5%, 0.25%, 0.125%, 0.0625%, and 0.03%. The dilution and diffusion testswere used to determine the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and zone of inhibition for eachtreatment material.Results: MICs of papain and Papacarie were 12.5%, indicating that at a concentration of 12.5%, the material can inhibit the growth of Streptococcusmutans. Papain does not have an MBC value but the Papacarie has an MBC at 25%, which indicating that at a concentration of 25%, Papacarie hasbactericidal effects on S. mutans. The zone of inhibition of papain was lower than Papacarie.Conclusion: Based on chemomechanical caries removal materials, the antimicrobial effects of Papacarie were better than those of papain.

MEMBRANE-ACTIVE METHANOLIC CRUDE EXTRACT OF THERMOTOLERANT, Aspergillus fumigatus SSH01 AND ITS MODE OF ACTION AGAINST GRAM-POSITIVE PATHOGENS

2017 ◽  
Vol 80 (1) ◽  
Author(s):  
Mohamad Khairil Radzali ◽  
Akmal Hayat Abdul Karim ◽  
Syahida Ahmad ◽  
Wan Zuhainis Saad
Keyword(s):  
Aspergillus Fumigatus ◽  
Crude Extract ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Properties ◽  
Bacterial Membrane ◽  
Antibacterial Effects ◽  
Gram Positive ◽  
Bacillus Subtilis Atcc ◽  
Time Kill

This study was undertaken to investigate the antibacterial properties and the mode of actions of crude extract of Aspergillus fumigatus SSH01. Antibacterial properties was observed against Gram-positive pathogens and showed inhibition against Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538, methicillin-resistant S. aureus S547 (MRSA) and Listeria monocytogenes L10 with minimum inhibitory concentration (MIC, 0.097- 12.5 mg/ml) and minimum bactericidal concentration (MBC, 0.195 – 25 mg/ml). No surviving cells were detected after 15 h of treatment with the 2MIC of extracts for time-kill assay. Leakage of cellular contents of the treated test pathogens were identified and increased as the concentrations of the extracts increased. The study of morphological surface has shown the bacterial membrane was disrupted and caused loss of viability. This implies the antibacterial effects of A. fumigatus SSH01 extract may serve as the potential antibiotic. 

Antibacterial Activity of Surface-Modified Fabric with Ag/AgCl-Doped Quaternary Ammonium-Modified Silicate Hybrid

2019 ◽  
Vol 19 (11) ◽  
pp. 7285-7293 ◽  
Author(s):  
Kuo-Hui Wu ◽  
Yin-Chiung Chang ◽  
Ken-Fa Cheng ◽  
Je-Chuang Wang
Keyword(s):  
Colloidal Suspension ◽  
Sol Gel ◽  
Antimicrobial Properties ◽  
Woven Fabrics ◽  
Dipolar Interactions ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Antibacterial Material ◽  
Surface Modified

An organic–inorganic hybrid antibacterial material based on Ag/AgCl and quaternary ammoniummodified silicate (Ormosil (NR+4 Cl-)) was prepared by sol–gel processes and an in situ reduction method, in which silver nitrate, tetraethoxysilane (TEOS), N-trimethoxysilylpropyl-N,N,Ntrimethylammonium chloride (TMAPS) and N-[3-(trimethoxysilyl)propyl]diethylenetriamine (ATS) acted as precursor, linker and colloidal suspension stabilizer, respectively. The physical properties of the Ormosil (NR+4 Cl-) and Ormosil (NR+4 Cl-)/Ag hybrids were examined using XPS and TGA spectroscopy, the results of which indicated that Ag/AgCl was immobilized in channels by dipolar interactions with the Ormosil (NR+4 Cl-) matrix. Ormosil (NR+4 Cl-) and Ormosil (NR+4 Cl-)/Ag sol solutions were used to functionalize non-woven fabrics by simply immersing the textiles into the dispersions in order to obtain antimicrobial fabrics. The antibacterial effects of the Ormosil/Ag- and Ormosil (NR+4 Cl-)/Ag-modified fabrics against Gram-negative P. aeruginosa and E. coli, and Grampositive S. aureus and B. subtilis, were assessed using the zone of inhibition and the plate-counting method. The antibacterial efficacy was maintained even after 20 laundry cycles. The results indicated that the Ormosil/Ag and Ormosil (NR+4 Cl-)/Ag hybrids are useful as non-leaching agents imparting durable antimicrobial properties to fabrics.

Pre-magnetization for enhancing the iron-catalyzed activation of peroxymonosulfate via accelerating the corrosion of Fe0

2019 ◽  
Vol 79 (7) ◽  
pp. 1287-1296 ◽  
Author(s):  
Yunxin Liu ◽  
Peng Zhou ◽  
Xiaowei Huo ◽  
Yang Liu ◽  
Xin Cheng ◽  
...  
Keyword(s):  
Field Gradient ◽  
Oxidation Process ◽  
Zero Valent Iron ◽  
Gradient Force ◽  
Acid Orange 7 ◽  
Lower Field ◽  
Acidic Conditions ◽  
Reactive Radicals ◽  
Better Than

Abstract Our findings proved that micron-scale zero-valent iron (mZVI) particles with pre-magnetization combined with peroxymonosulfate (PMS) can markedly enhance the removal of acid orange 7 (AO7). Investigation into the mechanism showed that PMS accelerated the corrosion of ZVI to release Fe2+ under acidic conditions, and the in-situ generated Fe2+ further activated PMS to produce SO4•− and •OH, resulting in AO7 removal. Further, the Lorentz force strengthened the convection in the solution and the field gradient force tended to move Fe2+ from a higher to a lower field gradient at the pre-magnetized ZVI (Pre-ZVI) particle surfaces, thus indicating that pre-magnetization promoted the corrosion of ZVI to release Fe2+, which resulted in the enhancement of PMS activation. Nano-scale ZVI (nZVI) was more effective than mZVI in activating PMS to degrade AO7, but the pre-magnetization effect on mZVI was better than on nZVI. AO7 removal increased with higher ZVI and PMS dosage, lower AO7 concentration, and acidic conditions (pH = 2, 3). This study helps to understand the reactive radicals-based oxidation process with application of pre-magnetized ZVI in activating PMS.

In-vitro Antibacterial Effects of Aqueous, Ethanolic, Methanolic, Acetone and Hydro Ethanolic Extracts of Achillea Millefolium on Standard Klebsiella, S. pyogenes, and Oral Bacterias Strains

2020 ◽  
Author(s):  
Negar Fallah ◽  
Sanaz Namazi ◽  
Negar Balmeh ◽  
Samira Mahmoudi ◽  
Fereshteh Mirzaei Poor
Keyword(s):  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Methanolic Extract ◽  
Growth Zone ◽  
Achillea Millefolium ◽  
Antibacterial Effects ◽  
Millipore Filter ◽  
Antimicrobial Effects ◽  
Aerial Parts

Introduction: So far, a lot of attempts have been carried out to find antimicrobial compounds. In this study, it was also tried to investigate the antibacterial effects of Achillea millefolium on standard Klebsiella, S. pyogenes, and oral bacterias strain. Material and Methods: The aerial parts of Achillea millefolium were used and the aqueous, ethanolic, methanolic, acetone and hydroethanolic extracts were prepared. After the preparation of standard strains of Klebsiella, S. pyogenes, and oral bacterias and sterilization of extracts by the Millipore filter, the antibacterial effects of these extracts on the mentioned microorganisms were assessed by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and well diffusion at the concentration 50 mg/ml. The test was repeated three times for each bacterium. Results: It was exposed that aqueous extract of Achillea millefolium had the most distinguished antimicrobial effects against all studied strains and methanolic extract had antimicrobial effects only on S. pyogenes. MIC and MBC of effective extracts were the basic concentration (50mg/ml), and non-growth zone was not observed in other serial dilution in case of all bacteria. Conclusion: The Achillea millefolium can be admitted as an antibacterial medicinal herb. Thus, it can be concluded that after evaluating their effects in vitro, Achillea millefolium can be utilized as an alternative to the routine chemical drugs.

scholarly journals Antimicrobial, Phytochemical Analysis and Molecular Docking (In-silico Approach) of Tithonia diversifolia (Hemsl.) A. Gray and Jatropha gossypiifolia L on Selected Clinical and Multi-Drug Resistant Isolates

2020 ◽  
pp. 1-18
Author(s):  
A. F. Okiti ◽  
O. T. Osuntokun
Keyword(s):  
Escherichia Coli ◽  
Molecular Docking ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Phytochemical Analysis ◽  
Drug Resistant ◽  
Tithonia Diversifolia ◽  
Zone Of Inhibition ◽  
Zones Of Inhibition ◽  
Jatropha Gossypiifolia

The aim of this study is to determine the zones of inhibition, phytochemical screening and molecular docking (In-silico Approach) of Tithonia diversifolia (Hemsl.) A. Gray and Jatropha gossypiifolia L against selected clinical and multi drug resistant isolates. Crude extraction of air dried leaves were carried out by soaking the plant in ethanol and ethyl acetate, standard agar diffusion method was used for sensitivity testing, minimum inhibitory concentration and minimum bactericidal concentration values were obtained by agar dilution method. The antimicrobial activity of the leaf extracts of T. diversifolia (Hemsl.) A. Gray and J. gossypiifolia L was assayed against Bacillus subtilis, Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysentriae, Staphylococcus aureus, Streptococcus pyogenes, Candida albican and against multi drug resistant bacteria which are Acinetobacter baumannii, Enterobacter agglomerans, Proteus mirabilis, Providencia stuartii, Salmonella subsp 3b. Levofloxacin and fluconazole were the standard antibiotics used. Sensitivity test revealed the highest zone of inhibition observed for J. gossypiifolia L and T. diversifolia (Hemsl.) A. Gray against Candida albican with mean and standard deviation of 29±1.414 and 19.5±0.707 at 100 mg/ml respectively, while the least zone of inhibition was observed from the extracts of J. gossypiifolia L against Escherichia coli with 11.75±0.354 at 100 mg/ml. Both plant extracts showed antimicrobial activity against multi drug resistant isolates having zones of inhibition ranging from 0 to 15±1.414. The Minimum Inhibitory Concentration of the extracts ranges between 6.25 and 100 mg/ml as well as the Minimum Bactericidal Concentration. The qualitative and quantitative phytochemical analysis showed the presence of alkaloids, anthraquinone, cardiac glycosides, flavonoids, phlobotannins, reducing sugars saponins, steroids and tannins. Molecular docking of the phytochemicals of T. diversifolia (Hemsl.) A. Gray only was carried out using levofloxacin as template, which revealed the presence of compounds more effective in inhibiting DNA gyrase enzyme. Thus, the use of both plants as traditional medicine is justifiable and should be encouraged in the formulation and production of new antibiotics.

Edible Thai rice film incorporated with ginger extract by microwave extraction: optimization of bioactive compounds and functional properties for antimicrobials

2021 ◽  
Author(s):  
Niramon Utama-ang ◽  
Sirinapa Sida ◽  
Phenphichar Wanachantararak ◽  
Arthitaya Kawee-ai
Keyword(s):  
Bioactive Compounds ◽  
Microwave Power ◽  
Inhibitory Concentration ◽  
Extraction Time ◽  
Edible Film ◽  
Zone Of Inhibition ◽  
Ginger Extract ◽  
Assisted Extraction ◽  
Full Factorial ◽  
Better Than

Abstract This study aimed to integrate microwave-assisted extraction (MAE) on the dry ginger extract and to develop rice-based edible film incorporated with ginger extract. An efficient MAE was developed to extract the dried ginger using a 32 full factorial design. The optimal condition was a microwave power of 400W and an extraction time of 1 min. The extraction time was a significantly effective factor than microwave power, whist power was not a significant factor for yield, 6-gingerol, 6-shogaol, and paradol. A crude extract of dried-ginger has antimicrobial activity against S. mutans DMST 18777 with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 0.49 mg/mL and 31.25 mg/mL, respectively. The rice-based edible film incorporated with 32 mg/mL of ginger extract against S. mutans DMST 18777 with a mean zone of inhibition of 12.69 ± 0.07 mm. The functional property of rice film was remarkably better than the original. Significant increases in TPC, antioxidant and bioactive compounds were associated with increase ginger extract contents in rice film. The main phenolic compounds including 6- gingerol 6-shogaol, paradol, and zingerone, and essential oils including α-curcumene, α-zingiberene, γ-muurolene, α-farnesene, β-bisabolene, and β-sesquiphellandrene were found in rice film strip fortify with crude ginger extract.

Antibacterial Activity Studies of Ni and SnO2 Loaded Chitosan Beads

2015 ◽  
Vol 832 ◽  
pp. 110-122 ◽  
Author(s):  
A. Ayeshamariam ◽  
G.V. Sankaracharyulu ◽  
M. Kashif ◽  
Shahid Hussain ◽  
Mohamed Bououdina ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Chitosan Nanoparticles ◽  
Chitosan Solution ◽  
Cross Linking ◽  
Chitosan Beads ◽  
Better Than

In this work, Ni and SnO2loaded Chitosan nanomaterials are prepared by neutralization method, obtained by drop wise addition of Chitosan solution to a solution of NaOH, followed by cross-linking. The Structural, morphological and Raman analyses are carried out. The antibacterial activity is analysed against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa and evaluated by the calculation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The obtained results show that, Ni-loaded nanoparticles could inhibit the growth of various bacteria better than SnO2. These results revealed that, the exposure of Staphylococcus aureus to the Chitosan nanoparticles led to the disruption of cell membranes and the leakage of cytoplasm.

scholarly journals Microbicidal activity of crude extracts from Sargassum wightii against Bacillus cereus

2014 ◽  
Vol 3 (10) ◽  
pp. 326-327 ◽  
Author(s):  
Amritha Krishna Bhuvaneshwari Venugopal ◽  
Vinoth Kumar Thirumalairaj ◽  
Geetharamani Durairaj ◽  
Mahitha Puthanpurayal Vijayan ◽  
Navya Lazar ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Brown Seaweed ◽  
Pharmaceutical Journal ◽  
Human Pathogen ◽  
Zone Of Inhibition ◽  
Microbicidal Activity ◽  
Crude Extracts

The present study was investigated to explore the antibacterial activity of four different solvent (petroleum ether, chloroform, acetone and ethanol) crude extracts of marine brown seaweed Sargassum wightti. Crude extracts were screened against human pathogen Bacillus cereus. The antibacterial efficiency was performed by agar well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. The results revealed that the crude extract of petroleum ether showed prominent inhibiting activity against B. cereus and other crude extracts showed below detectable level. The highest microbicidal activity (zone of inhibition) 9.0 ± 0.32 mm was obtained at the concentration of 250 ?g /ml and the lowest activity was 3 ± 0.20 mm at 31.25 ?g /ml concentration. The MIC and MBC values were found to be 125 and 250 ?g /ml respectively. Results of this study suggested that the compounds present in the crude extracts of petroleum ether showed high activity against B. cereus and further studies are required to purify the active principles.DOI: http://dx.doi.org/10.3329/icpj.v3i10.20338 International Current Pharmaceutical Journal, September 2014, 3(10): 326-327

scholarly journals Antibacterial Effects of Natural Herbal Extracts on Streptococcus mutans: Can They Be Potential Additives in Dentifrices?

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Spoorthi Banavar Ravi ◽  
Sudarshini Nirupad ◽  
Prashanthi Chippagiri ◽  
Rohit Pandurangappa
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Herbal Extracts ◽  
Zone Of Inhibition ◽  
Antibacterial Effects ◽  
Specific Test ◽  
Methanolic Extracts ◽  
Test Concentration

Background. Many plants or herbs exhibit potent antimicrobial activity against various microorganisms. They have no side effects and presumably act against and modulate the factors that are crucial for microbial survival or their activity. Streptococcus mutans is a pioneer bacteria implicated in dental caries. This study aims to evaluate the antimicrobial activity of garlic bulbs, pudina leaves, and mango and eucalyptus twig extracts on Streptococcus mutans by evaluating their zone of inhibition and determining their minimum inhibitory concentration (MIC). Methods. Microbiological assay (well diffusion method) to determine zone of inhibition against pure forms of Streptococcus mutans was performed. The antibacterial effects of methanolic extracts of mango twigs, eucalyptus twigs, pudina leaves, and garlic bulbs were studied. Test compounds were further evaluated for their MIC. Results. Extracts derived from mango and eucalyptus twigs showed significant antibacterial effects at test concentrations. Pudina and garlic extracts did not show any significant antibacterial effects at similar concentrations. Upon further evaluation of the 2 positive compounds for their MIC, mango twigs demonstrated more antimicrobial potential than eucalyptus twigs at a lower concentration. Conclusion. Our observations indicated that the mango twig extracts possess higher antibacterial effects against Streptococcus mutans than other compounds at specific test concentration.

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