How Many Bloods will a ‘HIVCHEK’ Check? Multiple Tests for HIV Antibody for a Single Screening Kit

1992 ◽  
Vol 22 (4) ◽  
pp. 151-154 ◽  
Author(s):  
G J Wannan ◽  
M J Cranefield ◽  
W A M Cutting ◽  
P R Fischer ◽  
F D Hargreaves ◽  
...  
Keyword(s):  
Blood Donors ◽  
Screening Tests ◽  
Serum Samples ◽  
Hiv Seroprevalence ◽  
Multiple Tests ◽  
Test Kit ◽  
Highly Sensitive ◽  
Multiple Sample ◽  
Sample Method ◽  
Hiv 1

Detection of HIV infection in blood donors or populations is usually by testing sera for antibodies to HIV-1 and HIV-2. Screening tests are now highly sensitive and specific, but still expensive and scarce in Africa. We tested the commercially available kits ‘HIVCHEK 1 + 2′ in two field laboratories, on specimens from blood donors and antenatal women in rural Zaire. We describe a method of using one test kit for up to five serum samples, saving money and time. In 491 antenatal mothers in Eastern Zaire, among whom the HIV seroprevalence was 3.3%, we compared ‘HIVCHEK’ results with results obtained by ELISA and Western blot. The ‘HIVCHEK’ multiple-sample method had a sensitivity of 82% and a specificity of 99.6%. In an area with an HIV seroprevalence of <4%, using ‘HIVCHEK’ by the multiple sample method would lead to a saving of about £2400 for every 1000 individuals tested.

scholarly journals Evaluation of positive serological screening test rates in blood donors with ABO and Rh type blood groups

2019 ◽  
Vol 6 (09) ◽  
pp. 4592-4595
Author(s):  
Canan Eren
Keyword(s):  
Blood Transfusion ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Blood Groups ◽  
Pathogen Transmission ◽  
Screening Tests ◽  
Serum Samples ◽  
Serological Screening ◽  
Significant Difference ◽  
Anti Hiv

Background: Blood transfusion is a life-saving method in emergencies. However, it also carries the risk of pathogen transmission, which is called transfusion transmitted infections (TTIs). All donated blood samples were screened for hepatitis B surface antigen (HBsAg), anti-hepatitis C virus antibody (anti-HCV), anti-human immunodeficiency virus antigens (anti-HIV½) and syphilis using Venereal Disease Research Laboratory (VDRL) test. This study was designed and carried out to determine the seroprevalences of TTIs among blood donors with different ABO and Rh blood groups. Identification of a relation between different blood groups and TTIs was anticipated. Methods: All blood donors data obtained from the Blood Center of Marmara University Pendik Training and Research Hospital between January 2013 and October 2018 are analyzed retrospectively. Serum samples were examined for HBsAg, anti-HCV, anti-HIV½, and VDRL. Results: HBsAg and anti-HIV½ positivity positivity rates were not statistically different according to ABO blood groups (p>0.05). Anti-HCV positivity rates are partially statistically significant (p<0.05). While the incidence of anti-HCV in patients with AB Rh (+) cases was significantly higher than that of 0 Rh (+), A Rh (+) and AB Rh (+) groups, no significant difference was found for the other analyzed blood groups (p>0.05). VDRL positivity rates did not differ significantly (p>0.05). Seropositive cases, as reported by Rh type blood group; HBsAg, anti-HCV, anti-HIV½, and VDRL positivity rates were not statistically significant (p>0.05). Conclusions: No significant relationship was found among TTIs and ABO and Rh type blood groups. For blood transfusion safety, screening tests should always and carefully be performed.

scholarly journals Prevalence of serum antibodies against bloodborne and sexually transmitted agents in selected groups in Somalia

2000 ◽  
Vol 124 (1) ◽  
pp. 137-141 ◽  
Author(s):  
Y. A. NUR ◽  
J. GROEN ◽  
A. M. ELMI ◽  
A. OTT ◽  
A. D. M. E. OSTERHAUS
Keyword(s):  
Civil War ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Hiv Infections ◽  
Hospitalized Children ◽  
Serum Samples ◽  
Hbv Vaccine ◽  
Sexually Transmitted ◽  
Human T Cell ◽  
Hiv 1

Somalia has suffered from a civil war during the last 10 years. In this period the use of whole blood has increased at least twofold in Mogadishu, Somalia compared with pre-war. Screening possibilities are limited. Recent data concerning the prevalence of infections with blood-borne and sexually transmitted agents are not available from this country. To investigate the spread of human immunodeficiency virus (HIV-1/2) and other blood-borne or sexually transmitted agents we tested a total of 256 serum samples collected in the summer of 1995 from blood donors, hospitalized children and adults in Mogadishu. The hepatitis B surface antigen (HbsAg) carrier rate was 19·1%, 5·6% and 21·3% among blood donors, hospitalized children and hospitalized adults, respectively. However, no children under 2 years of age were HbsAg positive. The overall presence of antibodies against hepatitis C virus (HCV) was 2·4% (6/256). In blood donors this was 0·6% (1/157). In none of the samples tested, antibodies against HIV 1 and 2 or human T-cell lymphotropic viruses (HTLV I and II) were detected. Our results indicate that, during the civil war in Somalia, no evidence of an increase of HIV infections was found. Our findings indicate that preventive measures in Somalia should focus mainly on prevention of HBV-infections. HBV-vaccine could be administered within the framework of the expanded programme on immunization, as none of the children less than 2 years of age were HbsAg positive.

scholarly journals Seroprevalence of markers of transfusion transmissible infections among blood donors at a tertiary care hospital blood bank: a 5 year retrospective study

2019 ◽  
Vol 7 (8) ◽  
pp. 2976
Author(s):  
Irm Yasmeen ◽  
Supinder Kour ◽  
Imran Khurshid ◽  
Aasifa Malik
Keyword(s):  
Blood Donors ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Blood Bank ◽  
Care Hospital ◽  
Serum Samples ◽  
Tertiary Care Centre ◽  
Healthy Donors ◽  
Hiv 1 ◽  
Apparently Healthy

 Background: Transfusion transmitted infections (TTIs) can be caused by various microorganisms present in the blood of apparently healthy donors. The recipient may get infected after being transfused with the unsafe blood. It is mandatory to screen the blood for HIV 1 and 2, HBV, HCV, Syphilis and Malaria. This study was undertaken to investigate the seroprevalance of Transfusion transmitted infections among blood donors at our tertiary care centre and to compare our study with other studies conducted at different hospitals of the country as well as outside.Methods: A retrospective review of blood donor records was made over a period of 5 years with effect from January 2014 to December 2018 at blood bank, department of Blood Transfusion and Immunohaematology, SKIMS, Soura, Srinagar. Both voluntary and replacement blood donors were selected after taking proper history and examination were included in the study. Serum samples were screened for HIV 1 & 2, HBV (HBsAg) and HCV using ELISA with 3rd generation kits.Results: A total of 56325 blood donors were screened. Out of total 55346 (98.2%) were males and 979 (1.73%) were females. Among them 38969 (69.1%) were replacement donors and 17356 (30.8%) were voluntary donors. The seroprevalance of HBV (HBsAg), HCV and HIV 1and 2 was 0.24%, 0.17% and 0.01% respectively and overall seroprevalance of TTIs was 0.43%.Conclusions: Continuous improvement and implementation of newer techniques like NAT and chemilumenescence for testing of blood for TTIs will reduce the risk of acquiring these infections.lance

scholarly journals Seroprevalence of transfusion transmitted infections in healthy blood donors: A 5-year tertiary care hospital experience

2017 ◽  
Vol 9 (04) ◽  
pp. 283-287 ◽  
Author(s):  
Sushama A. Chandekar ◽  
Gaythri P. Amonkar ◽  
Heena M. Desai ◽  
Nitin Valvi ◽  
Gururaj V. Puranik
Keyword(s):  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Venereal Disease ◽  
Tertiary Care ◽  
Screening Tests ◽  
Care Hospital ◽  
Serum Samples ◽  
Healthy Donors ◽  
Immunodeficiency Virus ◽  
Testing Technology

Abstract INTRODUCTION: Transfusion transmitted infections (TTIs) can cause threat to bloody safety as blood transfusion is an important mode of transmission of TTI to the recipient, hence, to prevent transmission of these diseases, screening tests on blood bags is an important step for blood safety. AIM: This study was undertaken with the aim of determining the seroprevalence of TTI in healthy blood donors in a tertiary care blood bank. MATERIALS AND METHODS: A retrospective study was carried out over a period of 5 years from January 2007 to December 2011. Serum samples were screened for hepatitis B surface antigen (HBsAg), antibodies to human immunodeficiency virus (HIV) Type 1 and 2, hepatitis c virus (HCV) and syphilis using enzyme-linked immunosorbent assays with the third generation kits and venereal disease research laboratory test, respectively. RESULTS: A total of 76,653 healthy donors were included out of which majority of donors were male (91.79%). The overall seroprevalence of HIV, HBsAg, HCV, and syphilis were 0.26%, 1.30%, 0.25%, and 0.28%, respectively. CONCLUSION: Methods to ensure a safety blood supply should be encouraged. For that, screening with a better selection of donors and use of sensitive screening tests including nucleic acid testing technology should be implemented.

scholarly journals Blood Borne Viral Infections in Transplantation: Hepatitis Viruses and Retroviruses

1993 ◽  
Vol 4 (suppl c) ◽  
pp. 20-25
Author(s):  
RP Bryce Larke
Keyword(s):  
Blood Donors ◽  
Hcv Infection ◽  
Residual Risk ◽  
Screening Tests ◽  
Hcv Rna ◽  
Type I ◽  
Hepatitis Viruses ◽  
Serological Screening ◽  
Window Period ◽  
Hiv 1

Hepatitis viruses B (HBV), C (HCV) and D (HDV) and the retroviruses human immunodeficiency virus (HIV-1) and human T celllymphotropic virus type I (HTLV-1) and type ll (HTLV-11) have been transmitted from infected organ and tissue donors to allograft recipients. Ascertainment of personal risk factors by health questionnaire may exclude volunteer blood donors recently exposed to transmissible diseases who could be in the 'window period' of the infection, when routine serological screening tests are negative. Difficulty in obtaining historical evidence of possible recent exposure from a critically ill prospective organ donor may make the residual risk of infection slightly higher than the risk estimated per unit of transfused products from serologically screened volunteer blood donors. Current estimates of residual risk from transfusion based on United States data are: one in 200,000 units for HBV; one in 2000 to one in 6000 units for HCV; one in 40.000 lo one in 60,000 units for HIV-1; and one in 69,272 units for HTLV-1/11. Despite recent improvements in anti-HCV testing, current screening assays underestimate the incidence of transmission and prevalence of HCV infection among immunosuppressed organ recipients: evidence of ongoing HCV infection depends on detection of HCV RNA by polymerase chain reaction. Determination of I-IIV-1 p24 antigen may facilitate identification of prospective organ donors in ll1e window period of early infection and may enhance serological follow-up of allograft recipients al risk of transplantation-associated HIV-1 infection. Highly sensitive assays that can be completed very rapidly are needed to ensure greater safely for the recipient of an emergency organ transplant, where time to screen a prospective donor for infectious diseases may be extremely limited.

scholarly journals Prevalence of HIV in Blood Donors

1970 ◽  
Vol 6 (2) ◽  
pp. 93-97 ◽  
Author(s):  
B R Tiwari ◽  
S Karki ◽  
P. Ghimire ◽  
P. Yadav ◽  
M Rajkarnikar
Keyword(s):  
Blood Transfusion ◽  
Blood Supply ◽  
Blood Donors ◽  
Blood Donation ◽  
Cumulative Effect ◽  
Serum Samples ◽  
Total Blood ◽  
Hiv Seroprevalence ◽  
Blood Transfusion Service ◽  
Safe Blood

Background: Nepal is categorized as a country with ‘concentrated' epidemic of HIV Nepal Red Cross Society, Blood Transfusion Service has implemented considerable efforts to supply the safe blood and blood products according to the increasing demand. A trend analysis might prove valuable to understand the outcome and appropriately plan for further  improvements in providing safe blood supply. The study aimed to assess the trends of HIV seroprevalence over the six years in nationwide and in urbanized setting of Kathmandu Valley.Methods: This is a retrospective study conducted among Nepalese blood donors through the years 2001-2007. Serum samples were tested using third generation ELISA tests, strictly following the instructions of manufacturers. The donors' information was collected through the donor record register through the respective Blood Transfusion Services.Results: The overall seroprevalence of HIV among the total blood donors in nation wide and in Central Blood Transfusion Service (CBTS), Kathmandu through the six years of review (from 2001-2007) was 0.33% and 0.4% respectively. A significant decreasing trend in HIV seroprevalence was observed both in nationwide and in Central Blood Transfusion Service, Kathmandu (P< 0.001).Conclusion: The analysis of trends in HIV seroprevalence among blood donors through the year 2001- 2007 showed a significantly decreasing trend, probably due to the cumulative effect of increasing awareness of HIV and improved screening system for safe blood donation. However, an abrupt increase in demand of blood supply was associated with significant increase in seroprevalence in the year 2005/2006. Key words: Nepalese blood donors; Seroprevalence; Trends.doi: 10.3126/jnhrc.v6i2.2203Journal of  Nepal Health Research Council 2008 Oct;6(13): Page: 93-97 

scholarly journals Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina

2001 ◽  
Vol 43 (5) ◽  
pp. 277-282 ◽  
Author(s):  
René GASTALDELLO ◽  
Sandra GALLEGO ◽  
María Beatriz ISA ◽  
Eduardo MATURANO ◽  
Santos SILEONI ◽  
...  
Keyword(s):  
High Risk ◽  
Blood Donors ◽  
Immunofluorescence Assay ◽  
High Rate ◽  
Infection Status ◽  
Serum Samples ◽  
The Status ◽  
High Risk Populations ◽  
Retrovirus Infection ◽  
Hiv 1

Serum samples (n: 110) from blood donors and high risk individuals from Cordoba, Argentina with indeterminate HIV-1 and HTLV-I/II Wb profiles were studied for specific antibodies to HTLV-I/II and HIV-1 by indirect immunofluorescence assay (IFA) and for the presence or absence of HIV-1 and HTLV-I/II specific bands by Wb. This study was carried out in order to characterize their putative reactions with HIV-1 and HTLV-I/II proteins and to resolve the retrovirus infection status of these individuals. Results indicated that blood donors sera displaying indeterminate HIV-1 or HTLV-I/II Wb patterns were not immunoreactive to HTLV-I/II and HIV-1 on IFA. However, a high rate of indeterminate HIV-1 and HTLV-I/II Wb samples from high risk individuals had positive HTLV-I/II and HIV-1 IFA results respectively. Our study supports the growing evidence that HTLV-HIV indeterminate seroreactivity in low risk population is due to a cross reaction against nonviral antigens, and in high risk populations the indeterminate samples show serological cross-recognition between HIV-1 proteins and HTLV-I/II proteins on Wb. These results point out the necessity to investigate the HTLV-I/II reactivity in indeterminate HIV-1 samples and viceversa in order to confirm the diagnosis. Finally, this study shows the potential usefulness of IFA in elucidating the status of HIV-1 and HTLV-I/II infection of individuals with indeterminate Wb profiles, thus enabling resolution of retrovirus infection status.

scholarly journals Prognostic Value of Rapid Test for Diagnosis of Dengue in Nepalese Patients during 2010 Epidemic

2012 ◽  
Vol 10 (1) ◽  
pp. 3-6 ◽  
Author(s):  
R Pun ◽  
Y Shah ◽  
GP Gupta ◽  
SP Sherchand ◽  
BD Pandey
Keyword(s):  
Early Diagnosis ◽  
Diagnostic Accuracy ◽  
Rapid Test ◽  
Immunochromatographic Test ◽  
Sample Collection ◽  
Serum Samples ◽  
Test Kit ◽  
Highly Sensitive ◽  
Vector Borne ◽  
Low K

Background Dengue is an emerging vector borne disease in Nepal and rapid diagnostic test is important for early diagnosis of the disease. Objectives The aim of the study was to evaluate the diagnostic accuracy of commonly used rapid immunochromatographic test kit in Nepal during 2010 dengue epidemic and to assess disease burden of dengue. Methods A total of 131 acute and nonacute serum samples were collected during recent epidemic of dengue in 2010 from clinically suspected Nepalese patients of different hospitals. Rapid immunochromatographic test kit was used for early diagnosis and enzyme immunosorbent was chosen as a reference assay. Results The sensitivity and specificity of rapid test was 70% and 76.54% respectively whereas the prevalence of the disease was 38.17%. The odds ratio for males was 1.8 however; the association with the disease was statistically not significant. Conclusion The diagnostic accuracy of rapid immunochromatographic test for dengue diagnosis was low (k=0.46). So, it should be substituted by highly sensitive test device for prompt diagnosis and health personnel should consider appropriate timing of sample collection for better performance of rapid test. KATHMANDU UNIVERSITY MEDICAL JOURNAL  VOL.10 | NO. 1 | ISSUE 37 | JAN - MAR 2012 | 7-10DOI: http://dx.doi.org/10.3126/kumj.v10i1.6905

Anticardiolipin Antibodies Are Elevated in HIV-1 Infected Haemophiliacs but Do Not Predict for Disease Progression

1989 ◽  
Vol 61 (01) ◽  
pp. 081-085 ◽  
Author(s):  
Simon Panzer ◽  
Christoph Stain ◽  
Hubert Hartl ◽  
Robert Dudczak ◽  
Klaus Lechner
Keyword(s):  
Normal Values ◽  
Anticardiolipin Antibodies ◽  
Haemophilia A ◽  
Serum Samples ◽  
Factor Viii Concentrates ◽  
Patient Groups ◽  
Anti Hiv ◽  
Hiv 1 ◽  
Cd4 Lymphocytes

SummaryLevels of anticardiolipin antibodies (ACA) were measured in 55 patients with haemophilia A in serum samples obtained in 1983 and in 1987. Twenty-one patients were negative for anti HIV-1 antibodies in 1983 and remained negative in 1987; 34 patients had anti HIV-1 antibodies in 1983; 17 of these latter patients remained asymptomatic, whereas 17 patients developed ARC or AIDS during the 4 years follow-up. Thirteen anti HIV-1 negative patients had elevated ACA levels in 1983; subsequently, a significant decrease was observed in all these subjects (p <0.001). All anti HIV-1 positive patients had elevated ACA levels in 1983; normal values were found in 9 patients in 1987. Yet, these changes were not significant (p >0.05). ACA levels were significantly higher in HIV-1 infected patients than in those without anti HIV-1 antibodies (p <0.05). There was no difference of ACA levels between the two anti HIV-1 positive patient groups, be it in 1983 or be it in 1987 (p >0.05). There was no correlation of ACA levels with serum IgG concentrations, CD4+ lymphocytes, or the consumption of factor VIII concentrates.

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