Microbiological Aspects of Naturally Occurring Primary Endodontic Infections in Dogs

2019 ◽  
Vol 36 (2) ◽  
pp. 124-128 ◽  
Author(s):  
Špela Srečnik ◽  
Irena Zdovc ◽  
Urška Javoršek ◽  
Tina Pirš ◽  
Zlatko Pavlica ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Opportunistic Pathogen ◽  
Ionization Time ◽  
Root Canals ◽  
Naturally Occurring ◽  
Endodontic Infections ◽  
Aseptic Conditions ◽  
Endodontic Disease ◽  
Microbiological Aspects

Dental fractures are common in dogs, but data on microbiology of naturally occurring primary endodontic infections, and their relation to clinical and radiographic signs, are lacking. Samples were obtained from root canals of 32 periodontally healthy fractured teeth under aseptic conditions and immediately cultured for aerobic and anaerobic bacteria. Cultures were further identified by matrix-assisted laser desorption/ionization, time of flight technology. Sixty-one bacteria (30 bacterial species) were isolated from root canals; 54% were Gram-negative bacteria, 53% were facultative anaerobic, and 42% were anaerobic bacteria. Number of bacterial species in the root canals declined with the duration of fractures over 12 months. No statistically significant association was found between the number of bacterial species involved in the root canal infection and any of the clinical or radiographic signs of endodontic disease. Naturally occurring primary endodontic infections in dogs appear to be polymicrobial and involve only a selected number of opportunistic pathogen species.

scholarly journals A review of microbiological root canal sampling: updating an emerging picture

2013 ◽  
Vol 9 (1) ◽  
Author(s):  
Ana Moura Teles ◽  
M. Conceição Manso ◽  
Cristina Pina ◽  
José Cabeda
Keyword(s):  
Root Canal ◽  
English Language ◽  
Anaerobic Bacteria ◽  
Sample Collection ◽  
Scientific Rigor ◽  
Root Canals ◽  
Additional Information ◽  
Endodontic Infections ◽  
Language Accessibility ◽  
Positive Results

Objectives: The significance of microorganisms in root canals with regard to the aetiology of periapical infection and the need for crucial bacteria control during treatment are undeniable. In this study, we re-port and discuss a review of the literature on Microbiological Root Canal Sampling (MRS). The procedure is analyzed in detail, discussing its powers, limitations and the influence of sample collection procedures on the incidence of true and false positive results. Data sources: MEDLINE/PUBMED, B-On and library files of Oporto University were accessed. Selection: Papers were selected using the keywords: “root ca¬nal sampling”; “apical periodontitis”; “endodontic pathogens”; “root canal infection”; “Culture”; “molecular biology”. The references were selected under inclusion criteria such as English language, accessibility, rel¬evance to the theme and scientific rigor. Conclusions: This review illustrated the absolute need to adhere to strict methodology procedures if valid samples are to be obtained. A combination of Culture and molecular identification approaches have confirmed the polymicrobial nature of endodontic infections with a pre¬dominance of anaerobic bacteria. Nucleic acid-based techniques provide significant additional information particularly regarding the not-yet-cultivable species of the microbial community, but greatly increase the budget of the procedure. Thus, assessment of the endodontic microflora, in the context of a polymicro¬bial biofilm ecosystem, and its relevance to endodontic treatments must rely in the complementariness of Culture and Metagenomics approaches as they are neither mutually exclusive nor competitive, but strongly complementary.

Presence of Oral Bacterial Species in Primary Endodontic Infections of Primary Teeth

2013 ◽  
Vol 38 (2) ◽  
pp. 155-160 ◽  
Author(s):  
N Topcuoglu ◽  
E Bozdoğan ◽  
O Aktoren ◽  
G Kulekci
Keyword(s):  
Primary Teeth ◽  
Bacterial Species ◽  
Treatment Strategies ◽  
Fusobacterium Nucleatum ◽  
Primary Tooth ◽  
Microarray Technology ◽  
Microbial Composition ◽  
Root Canals ◽  
Endodontic Infections ◽  
Clinical Conditions

Objective: Knowledge of the microbial composition of deciduous endodontic infections is limited. This study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions. Study Design: The samples were collected from 30 root canals of primary teeth with primer infection. The bacterial composition of the samples was semi-quantitatively defined using a microarray system (Parocheck®). Results: All the tested species were detected in the samples. Fusobacterium nucleatum was the most frequently isolated bacterium (96.7%), followed by Prevotella intermedia (86.7%), Parvimonas micra (83.3%), Treponema denticola (76.7%) and Tannerella forsythia (66.7%). These bacteria were also present in high levels. All pairs of bacterial species were positively associated (RR≯1), except P.intermedia and P.micra. On average, five species (range:3-8) were detected per amplified sample. Root canals of teeth with ≯5 different species were statistically associated with periapical radiolucency (P=0.049). Conclusions: Primary teeth with endodontic infections show a highly diverse variety of bacteria, in which the most prevalent specie are present in high proportions. The well-directed use of the improved microarray technology will provide additional valuable information for causative factors associated with endodontic diseases, helping to develop more successful antibacterial or anti-inflammatory treatment strategies.

Identification of Cultivable Microorganisms from Primary Teeth with Necrotic Pulps

2010 ◽  
Vol 34 (4) ◽  
pp. 329-333 ◽  
Author(s):  
Gildardo Ledezma-Rasillo ◽  
Hector Flores-Reyes ◽  
Ana Ma. Gonzalez-Amaro ◽  
Arturo Garrocho-Rangel ◽  
Ma. del Socorro Ruiz-Rodriguez ◽  
...  
Keyword(s):  
Root Canal ◽  
Primary Teeth ◽  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Sinus Tract ◽  
Operative Field ◽  
Root Canals ◽  
Carious Lesions ◽  
Oral Environment ◽  
Clostridium Spp

The objective of this study was to identify cultivable microorganisms from primary teeth with necrotic pulps. This experimental study included 21 patients of both sexes between 4 and 7 years of age with necrotic pulps in primary teeth. Twenty-one maxillary and mandibular molars containing at least 1 necrotic canal, an abscess or sinus tract, one or more radiolucent areas in the furcation or periapical region, teeth having at least two thirds of root length, and carious lesions directly exposed to the oral environment were included. After antisepsis of the oral cavity, anesthesia of the affected tooth, and isolation and disinfection of the operative field, 3 sterile absorbent paper points were sequentially placed for 30 seconds for the collection of samples. The samples were immediately processed in an anaerobic chamber, and all isolated microorganisms were identified. Anaerobic species (anaerobic facultative and moderate anaerobes) were isolated in all root canals; 68.4% of root canal samples studied showed a polymicrobial nature. Most of the isolate consisted of Bifidobacterium Spp2 and Streptococcus intermedius. Other less frequently encountered species were Actinomyces israelii, Bifidobacterium spp 1, Clostridium spp, and Candida albicans. Results indicate the existence of combinations of bacterial species in root canal infections of the primary dentition with necrotic pulps, anaerobic bacteria predominating.

scholarly journals Antibacterial activity of calcium hydroxide and chlorhexidine containing points against Fusobacterium nucleatum and Parvimonas micra

2012 ◽  
Vol 06 (04) ◽  
pp. 434-439
Author(s):  
Andreas Rathke ◽  
Dominik Meisohle ◽  
Jens Bokelmann ◽  
Bernd Haller
Keyword(s):  
Calcium Hydroxide ◽  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Fusobacterium Nucleatum ◽  
Inhibitory Effect ◽  
Anaerobic Incubation ◽  
Root Canals ◽  
Parvimonas Micra ◽  
Vitro Model

ABSTRACTObjective: Up to 90% of the flora of an infected root canal consists of obligate anaerobic bacteria. However, most studies have centred on microaerophiles. This quantitative assay investigated the antibacterial effects against Fusobacterium nucleatum and Parvimonas micra of gutta-percha (GP) points containing either calcium hydroxide (Ca(OH)2) or chlorhexidine (CHX) compared to those of conventional GP points.Methods: Standardized root canals were prepared in 192 bovine incisors. The canals were sterilized and then inoculated with one of two bacterial species (n=96 teeth per bacterium). After anaerobic incubation at 37ºC, the inoculum was removed and the canals were filled with one of the three points. Control teeth were filled with a saline/serum mixture. After 0, 1, 4 and 8 days of anaerobic incubation, the numbers of viable bacteria (CFU/ml) were determined on both the points and the canal wall dentin. Six replicates were carried out for each experiment.Results: None of the points completely eradicated the bacteria in the canal. GP+CHX was significantly more effective than GP+Ca(OH)2 and GP (P<.05 in each case). The inhibitory effect of GP+Ca(OH)2 did not significantly differ from that of GP (P>.05). Compared to the controls, the antibacterial effect of the medicated points was reached initially.Conclusion: Within the limitations of the in vitro model, the incorporation of commonly used medicaments in GP does not offer a long-lasting antibacterial advantage over non-medicated GP. (Eur J Dent 2012;6:434-439)

scholarly journals No Change, No Life? What We Know about Phase Variation in Staphylococcus aureus

2021 ◽  
Vol 9 (2) ◽  
pp. 244
Author(s):  
Vishal Gor ◽  
Ryosuke L. Ohniwa ◽  
Kazuya Morikawa
Keyword(s):  
Gene Expression ◽  
Staphylococcus Aureus ◽  
High Frequency ◽  
Direct Evidence ◽  
Bacterial Species ◽  
Phase Variation ◽  
Opportunistic Pathogen ◽  
Adaptation Strategy ◽  
Innate Immune ◽  
Epigenetic Mechanisms

Phase variation (PV) is a well-known phenomenon of high-frequency reversible gene-expression switching. PV arises from genetic and epigenetic mechanisms and confers a range of benefits to bacteria, constituting both an innate immune strategy to infection from bacteriophages as well as an adaptation strategy within an infected host. PV has been well-characterized in numerous bacterial species; however, there is limited direct evidence of PV in the human opportunistic pathogen Staphylococcus aureus. This review provides an overview of the mechanisms that generate PV and focuses on earlier and recent findings of PV in S. aureus, with a brief look at the future of the field.

scholarly journals Pneumonia due to Pandoraea Apista after evacuation of traumatic intracranial hematomas:a case report and literature review

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Chuanzhong Lin ◽  
Ning Luo ◽  
Qiang Xu ◽  
Jianjun Zhang ◽  
Mengting Cai ◽  
...  
Keyword(s):  
Opportunistic Pathogen ◽  
Multidrug Resistant ◽  
Multi Drug Resistance ◽  
Emerging Pathogen ◽  
Case Presentation ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Antibiotic Susceptibility Test ◽  
Chinese Physicians ◽  
Level Identification

Abstract Background Pandoraea species is a newly described genus, which is multidrug resistant and difficult to identify. Clinical isolates are mostly cultured from cystic fibrosis (CF) patients. CF is a rare disease in China, which makes Pandoraea a total stranger to Chinese physicians. Pandoraea genus is reported as an emerging pathogen in CF patients in most cases. However, there are few pieces of evidence that confirm Pandoraea can be more virulent in non-CF patients. The pathogenicity of Pandoraea genus is poorly understood, as well as its treatment. The incidence of Pandoraea induced infection in non-CF patients may be underestimated and it’s important to identify and understand these organisms. Case presentation We report a 44-years-old man who suffered from pneumonia and died eventually. Before his condition deteriorated, a Gram-negative bacilli was cultured from his sputum and identified as Pandoraea Apista by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS). Conclusion Pandoraea spp. is an emerging opportunistic pathogen. The incidences of Pandoraea related infection in non-CF patients may be underestimated due to the difficulty of identification. All strains of Pandoraea show multi-drug resistance and highly variable susceptibility. To better treatment, species-level identification and antibiotic susceptibility test are necessary.

scholarly journals Bifidobacterium adolescentis shows potential to strengthen host defence against gastrointestinal infection via inhibition of the opportunistic pathogen Candida albicans and stimulation of human-isolated macrophages killing capacity in vitro

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Liviana Ricci ◽  
Joanna Mackie ◽  
Megan D. Lenardon ◽  
Caitlin Jukes ◽  
Ahmed N. Hegazy ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Bacterial Species ◽  
Opportunistic Pathogen ◽  
Limited Information ◽  
Human Gut ◽  
Bifidobacterium Adolescentis ◽  
Host Immune Responses ◽  
Opportunistic Fungal Pathogen ◽  
Antimicrobial Metabolites

The human gut microbiota enhances the host’s resistance to enteric pathogens via colonisation resistance, a phenomenon that is driven by multiple mechanisms, such as production of antimicrobial metabolites and activation of host immune responses. However, there is limited information on how individual gut bacterial species, particularly many of the dominant anaerobes, might impact the host’s defence. This study investigated the potential of specific human gut isolates to bolster the host’s resistance to infection. First, by antagonising the opportunistic fungal pathogen Candida albicans, and secondly, by modulating the killing capacity of human-isolated macrophages in vitro. Co-culturing C. albicans with faecal microbiota from different healthy individuals revealed varying levels of fungal inhibition. In vitro assays with a panel of representative human gut anaerobes confirmed that culture supernatants from certain bacterial isolates, in particular of Bifidobacterium adolescentis, significantly inhibited C. albicans growth. Mechanistic studies revealed that microbial fermentation acids including acetate and lactate, in combination with the associated decrease in pH, were strong drivers of this inhibitory activity. In the second in vitro assay, human-isolated macrophages were exposed to bacterial supernatants, and subsequently tested for their capacity to eliminate adherent-invasive Escherichia coli. Among the gut anaerobes tested, B. adolescentis was revealed to exert the strongest immunostimulatory and killing effect when compared to the unstimulated macrophages control. B. adolescentis is known to be stimulated by dietary consumption of resistant starch andmay therefore represent an attractive target for the development of probiotic and prebiotic interventions tailored to enhancethe host’s natural defences against infection.

scholarly journals Identification and Quantification by Targeted Metabolomics of Antibiotic-Responsive Urinary Small Phenolic Molecules Derived from the Intestinal Microbiota in Mice

2019 ◽  
Vol 4 (1) ◽  
pp. 85
Author(s):  
Mark E. Obrenovich ◽  
George Eugene Jaskiw ◽  
Renliang Zhang ◽  
Belinda Willard ◽  
Curtis J. Donskey
Keyword(s):  
High Performance ◽  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Liquid Chromatography Mass Spectrometry ◽  
Targeted Metabolomics ◽  
Mouse Urine ◽  
Urinary Levels ◽  
Low Levels ◽  
Pathogen Colonization ◽  
The Relationship

Background: Urinary levels of small molecules generated by the gut microbiota (GMB) constitute potential biomarkers for the state of the GMB. Such metabolites include numerous small phenolic molecules linked to anaerobic bacteria, particularly Clostridium species. Due to multiple technical challenges, however, the relationship between these chemicals and the GMB remains poorly characterized. Improved, high-performance liquid chromatography-mass spectrometry (LC-MS)-based metabolomic analysis can now reliably separate and quantify low levels of multiple small phenolic molecules and their structural isomers.Methods: CF-1 (female mice) were treated over 2 consecutive days with either i) vehicle, ii) one of 2 different antibiotic regimens (clindamycin or piperacillin/tazobactam) known to inhibit intestinal anaerobes and promote colonization by Clostridium difficile and other pathogens or iii) an antibiotic (aztreonam) that suppresses facultative Gram-negative bacteria but not enterococci or anaerobes and does not promote pathogen colonization Urine collected 24 hours after the last treatment was analyzed by LC-MS.Results: We identified over 25 compounds, many of which had not been previously reported in mouse urine. Eleven small phenolic molecules showed significant antibiotic-related changes. Urinary levels of the hydroxyphenylpropionic acids were suppressed by clindamycin and piperacillin/tazobactam treatment, but were elevated in aztreonam-treated mice. In addition, aztreonam treatment was associated with elevated levels of the dihydroxyhydrocinnamic acids.Conclusions: Profiles of differential changes in urinary small phenolic molecules may provide an index of anaerobic bacterial species in the GMB and could prove useful in monitoring susceptibility to overgrowth of pathogens such as C. difficile.

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