scholarly journals Exposure to a “safe” dose of environmental pollutant bisphenol A elevates oxidative stress and modulates vasoactive system in hypertensive rats

2021 ◽  
pp. 096032712110532
Author(s):  
Manigandan Nagarajan ◽  
Boobalan Raja ◽  
Jeganathan Manivannan
Keyword(s):  
Adverse Effects ◽  
Bisphenol A ◽  
Thiobarbituric Acid ◽  
Environmental Pollutant ◽  
Ros Generation ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hypertensive Rats ◽  
Liver And Kidney ◽  
Ace Activity ◽  
Safe Dose

Due to the prevalence of hypertension (one of the major risk factors of CVD) in the population, it is necessary to explore the adverse effects of daily tolerable and “safe” dose of bisphenol A (BPA) under hypertensive conditions. The current study exposed the Nω-nitro-l-arginine methyl ester (L-NAME, 40 mg/kg b.w/day) induced hypertensive Wistar rats to BPA (50 μg/kg b.w/day) by oral administration along with appropriate controls for 30 days period. The results illustrate that a ‘safe’ dose of BPA does not influence the systolic blood pressure (SBP) and levels of circulatory biomarkers of tissue damage. On the other hand, BPA exposure significantly ( p < 0.05) elevates the thiobarbituric acid reactive substances (TBARS) content in plasma and tissues (heart, aorta, liver and kidney) in hypertensive rats when compared with respective control (BPA alone exposed) rats. Similarly, a significant modulation of ROS generation in RBC, plasma nitric oxide (NO) level and angiotensin-converting enzyme (ACE) activity was observed only under hypertensive milieu. In conclusion, the observed adverse effects during ‘safe’ dose of BPA exposure are specific to the hypertensive condition. Therefore, a precise investigation to explore the effects of BPA exposure in vulnerable hypertensive populations is highly suggested.

scholarly journals D-α-tocopherol reduces renal damage in hypertensive rats

2012 ◽  
Vol 48 (2) ◽  
pp. 291-298 ◽  
Author(s):  
Thaís Maria da Fonseca Pletiskaitz ◽  
Guiomar Nascimento Gomes
Keyword(s):  
Renal Damage ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Functional Changes ◽  
Beneficial Effects ◽  
Renal Vascular ◽  
Volume Retention ◽  
Normotensive Control

This study investigated the beneficial effects of D-α-tocopherol supplementation in protecting against the renal morphological and functional changes caused by hypertension. Spontaneously hypertensive (SHR) and normotensive control (WKY) rats received D-α-tocopherol (80 mg/kg by gavage) or vehicle (mineral oil) every other day for 60 days, from the age of 2 months. After this treatment period, all animals were assessed for renal morphological and functional parameters. The glomerular hypertrophy, increased interlobular wall thickness and enlarged renal vascular resistance found in SHR were reduced by D-α-tocopherol treatment. Sodium and volume retention observed in SHR were also decreased by D-α-tocopherol treatment. Moreover, D-α-tocopherol supplementation significantly reduced arterial pressure in SHR but not in WKY. D-α-tocopherol also reduced the excretion of thiobarbituric acid-reactive substances (TBARS), a marker of oxidative stress, in SHR. These results suggest that D-α-tocopherol supplementation can reduce kidney damage induced by hypertension.

scholarly journals ANTIOXIDATIVE DEFENSE RESPONSE TO APHID-INDUCED OXIDATIVE STRESS IN Glycine max (L.) Merr. cv. “Nam Dan”

2016 ◽  
Vol 54 (6) ◽  
pp. 719 ◽  
Author(s):  
Tran Ngoc Toan ◽  
Tran Thi Van Thanh Huyen ◽  
Mai Van Chung
Keyword(s):  
Oxidative Stress ◽  
Glycine Max ◽  
Oxidative Damage ◽  
Thiobarbituric Acid ◽  
Ros Generation ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Aphis Craccivora Koch ◽  
Glycine Max L ◽  
Aphid Feeding

Infestation of cowpea aphid (Aphis craccivora Koch) induced oxidative stress in leaves of soybean (Glycine max (L.) Merr. cv. “Nam Dan”) with a burst in generation of reactive oxygen species (ROS) products such as superoxide anion radical (O2.-) and hydrogen peroxide (H2O2) recorded around 24 hours after aphid feeding. An increase in content of thiobarbituric acid reactive substances (TBARS) in lipid peroxidation and a defined percentage of injury in aphid-infested leaves were resulted from the cellular oxidative damage. The enhanced activity of the antioxidant enzymes such as superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6) in leaves of soybean “Nam Dan” functions as the antioxidative response that controlled both ROS-generation to be enough levels to play as defensive element and ROS-detoxifying to reduce aphid-induced oxidative damage. The enhancement of SOD and CAT also can improve the tolerance of soybean “Nam Dan” to impact from A. craccivora.

scholarly journals Antioxidant Action of Solid Preparation of Xingnaojing in SHRSP

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yang Liu ◽  
Naomi Yasui ◽  
Aya Kishimoto ◽  
Jian-ning Sun ◽  
Katsumi Ikeda
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Antioxidant Effect ◽  
Antioxidant Action ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Total Antioxidant

We investigated the antioxidant action of a solid preparation of Xingnaojing (XNJ) and ascorbic acid (AA) in stroke-prone spontaneously hypertensive rats (SHRSP). The total antioxidant states in the plasma, systolic blood pressure, and heart rate were measured every 2 weeks, and lipid peroxidation, expressed as thiobarbituric acid-reactive substances in plasma, was measured in the 6th week. The results showed that AA and XNJ significantly increased the total antioxidant status in plasma and reduced malondialdehyde in the plasma. These data suggest that during 6-week administration, XNJ has antioxidant action on SHRSP, which may relate to its generalized inhibition of lipid peroxidation and promotion of the total antioxidant state. These results demonstrated that orally treated XNJ has an antioxidant effect on SHRSP plasma.

Oral supplementation with the rutin improves cardiovagal baroreflex sensitivity and vascular reactivity in hypertensive rats

2013 ◽  
Vol 38 (11) ◽  
pp. 1099-1106 ◽  
Author(s):  
Leônidas das Graças Mendes-Junior ◽  
Matheus Morais de Oliveira Monteiro ◽  
Alynne dos Santos Carvalho ◽  
Thyago Moreira de Queiroz ◽  
Valdir de Andrade Braga
Keyword(s):  
Oxidative Stress ◽  
Sodium Nitroprusside ◽  
Baroreflex Sensitivity ◽  
Vascular Reactivity ◽  
Thiobarbituric Acid ◽  
Sensitivity Test ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hypertensive Rats ◽  
Oral Supplementation

The hypothesis that oral supplementation with the flavonoid rutin improves baroreflex sensitivity and vascular reactivity in hypertensive (2-kidney-1-clip (2K1C)) rats was tested. Sixty-four rats were divided in 4 groups: sham + saline; sham + rutin; 2K1C + saline, and 2K1C + rutin. Six weeks after 2K1C surgery, the animals were treated with saline or rutin (40 mg·kg−1·day−1) by gavage for 7 days. Baroreflex sensitivity test using phenylephrine (8 μg·kg−1, iv) and sodium nitroprusside (25 μg·kg−1, iv), vascular reactivity, and thiobarbituric acid reactive substances assay were performed. Baroreflex sensitivity in hypertensive rats was impaired and compared with sham (−2.77 ± 0.15 vs. –1.53 ± 0.27 beats·min−1·mm Hg−1; n = 8; p < 0.05). Oral supplementation with rutin restored baroreflex sensitivity in 2K1C rats (−2.40 ± 0.24 vs. –2.77 ± 0.15 beats·min−1·mm Hg−1; n = 8; p > 0.05). Besides, hypertensive rats have greater contraction to phenylephrine (129.49% ± 4.46% vs. 99.50% ± 11.36%; n = 8; p < 0.05), which was restored by rutin (99.10% ± 1.77% vs. 99.50% ± 11.36%; n = 8; p > 0.05). Furthermore, vasorelaxation to acetylcholine was diminished in hypertensive rats (96.42% ± 2.80% vs. 119.35% ± 5.60%; n = 8; p < 0.05), which was also restored by rutin (117.55% ± 6.94% vs. 119.35% ± 5.60%; n = 8; p > 0.05). Finally, oxidative stress was greater in hypertensive rats (1.54 ± 0.12 vs. 0.53 ± 0.12 nmol MDA·mL−1; n = 8; p < 0.05) and rutin supplementation significantly decreased oxidative stress in those animals (0.70 ± 0.13 vs. 1.54 ± 0.12 nmol MDA·mL−1; n = 8; p < 0.05). We concluded that oral supplementation with rutin restores impaired baroreflex sensitivity and vascular reactivity in hypertensive rats by decreasing oxidative stress.

scholarly journals Codeine-mediated Haematoxicity, Hepatotoxicity and Nephrotoxicity in Male Albino Rats

2019 ◽  
pp. 1-10
Author(s):  
Abiodun Olusoji Owoade ◽  
Abdulrahman Abdullateef ◽  
Adewale Adetutu ◽  
Adewale Adetutu ◽  
Olubukola Sinbad Olorunnisola
Keyword(s):  
White Blood Cells ◽  
Thiobarbituric Acid ◽  
Albino Rats ◽  
High Dose ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Levels ◽  
Liver And Kidney ◽  
Mean Corpuscular Haemoglobin ◽  
Endogenous Antioxidant ◽  
Biochemical Indices

Objectives: This study aimed to investigate the effects of codeine administration on some haematological and biochemical indices in rats. Materials and Methods: Therapeutic dose (5 mg/kg/day), high dose (25 mg/kg/day) and extreme dose (50 mg/kg/day) of codeine were administered orally to rats for 28 days. Twenty-four hours after the last codeine administration, blood, liver and kidney were removed from the animals after an overnight fast and analysed for their haematological and biochemical parameters. Results: Results obtained revealed that codeine administration significantly reduced the levels of white blood cells (WBC), red blood cell (RBC) and platelet count (PLT) and increased the levels of mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV)while it resulted in non-significant changes in other haematological parameters examined when compared with control rats. Codeine intake significantly increased plasma levels of alkaline phosphatase (ALP), aspartate aminotransferase (AST), creatinine and urea while its reduced total protein levels. Hepatic and renal thiobarbituric acid reactive substances (TBARS) levels were significantly increased by codeine administration while levels of endogenous antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) were reduced. Conclusions: This study confirmed the risk of increased oxidative stress, haematoxicity, hepatotoxicity and nephrotoxicity due to codeine administration. Although codeine is reported to be effective in pain management, its toxicity should be kept in mind.

The ameliorating effect of Centella asiatica ethanolic extract on albino rats treated with isoniazid

2017 ◽  
Vol 28 (1) ◽  
Author(s):  
Kavisa Ghosh ◽  
N. Indra ◽  
G. Jagadeesan
Keyword(s):  
Reduced Glutathione ◽  
Ethanolic Extract ◽  
Centella Asiatica ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Effective Concentration ◽  
Albino Rats ◽  
Thiobarbituric Acid Reactive Substances ◽  
Histological Studies ◽  
Liver And Kidney

AbstractBackground:Isoniazid, also called isonicotinyl hydrazine (INH), is a commonly used drug for treating tuberculosis. The main drawback is its toxic side effects.Methods:The adverse effects induced by INH (50 mg/kg bw) administration on haematological parameters, oxidative status (thiobarbituric acid-reactive substances, superoxide dismutase, catalase, and reduced glutathione), liver and kidney function markers, and their amelioration by various concentrations of CA (20, 40, 60, and 100 mg/kg bw) or silymarin (SIL) (50 mg/kg bw, administered before 1 h of INH treatment for 30 days to rats) were studied. Moreover, histological studies were carried out in liver and kidney tissues of rats treated with the most effective concentration to further support the possible effectiveness of CA on INH-intoxicated rats.Results:All the affected parameters returned to near-normal levels, and the effective concentration of extract was found to be 100 mg/kg bw. The histology of both the liver and the kidneys subsequently supported the effectiveness of CA (100 mg/kg bw).Conclusions:Altogether, the results suggest that CA at 100 mg/kg bw can substantially reduce the toxic effects of INH.

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

Melatonin protects against cadmium-induced oxidative damage in different tissues of rat: a mechanistic insight

2019 ◽  
Vol 2 (2) ◽  
pp. 1-21 ◽  
Author(s):  
Elina Mitra ◽  
Bharati Bhattacharjee ◽  
Palash Kumar Pal ◽  
Arnab Kumar Ghosh ◽  
Sanatan Mishra ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Oxidative Damage ◽  
Protein Carbonyl ◽  
Environmental Pollutant ◽  
Glutathione S Transferase ◽  
Protein Carbonyl Content ◽  
Wide Range ◽  
Liver And Kidney ◽  
Nadh Cytochrome ◽  
Cd Exposure

Cadmium (Cd) is a notorious environmental pollutant known for its wide range of toxicities to organisms. Thus, the present study is designed to examine whether melatonin, a potent antioxidant, protects against Cd-induced oxidative damage in the heart, liver and kidney of rats. Cd treatment at a dose of 0.44 mg/kg for 15 days caused severe damage in all these organs. These included significantly increased activities of SGPT, SGOT, lactate dehydrogenase- 1 and 5 and ALP and levels of total lactate, creatinine, lipid peroxidation, protein carbonyl content and reduced glutathione while the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase along with mitochondrial pyruvate dehydrogenase, isocitrate dehydrogenase, α-keto glutarate dehydrogenase, succinate dehydrogenase, NADH-cytochrome-c-oxidoreductase and cytochrome-c-oxidase were significantly reduced by Cd. However, if melatonin was given orally 30 min before Cd injection, all these alterations induced by Cd were significantly preserved by melatonin. Histological observations also demonstrated that Cd exposure caused cellular lesions, promoting necrotic or apoptotic changes. Notably, all these changes were significantly protected by melatonin. The results suggest that melatonin is a beneficial molecule to ameliorate Cd-induced oxidative damage in the heart, liver and kidney tissues of rats with its powerful antioxidant capacity, heavy metal chelating activity and competition of binding sites with Cd to the GSH and catalase.

Association Between Magnesium and Oxidative Stress in Patients with Obesity

2020 ◽  
Vol 16 (5) ◽  
pp. 743-748
Author(s):  
Ana R.S. de Oliveira ◽  
Kyria J.C. Cruz ◽  
Jennifer B.S. Morais ◽  
Juliana S. Severo ◽  
Jéssica B. Beserra ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Magnesium Concentration ◽  
Control Group ◽  
Compensatory Mechanism ◽  
Thiobarbituric Acid Reactive Substances ◽  
Magnesium Intake ◽  
Significant Difference ◽  
Dietary Magnesium ◽  
And Control

Background: The role of minerals in preventing the generation of oxidative stress in obese individuals has been evaluated. Magnesium is an antioxidant nutrient and a cofactor of enzymes involved in the cell membrane stabilization, attenuating the effects of oxidative stress. Objective: To evaluate the association between magnesium and concentrations of thiobarbituric acid reactive substances (TBARS) in patients with obesity and eutrophic women. Methods: A cross-sectional study was conducted with 73 women, divided into two groups: case group (patients with obesity, n=27) and control group (eutrophic women, n=46). Measurements of body mass index and waist circumference were performed. Dietary magnesium intake was assessed by the three-day food record using the NutWin software. Urinary magnesium concentration was measured by atomic absorption spectrophotometry method. Plasma concentrations of thiobarbituric acid reactive substances (TBARS) were also determined. Results: Mean values of dietary magnesium intake were 161.59 ± 60.04 and 158.73 ± 31.96 for patients with obesity and control group, respectively, with no significant difference between the groups studied (p >0.05). The value of urinary excretion of magnesium was lower than the reference values in both groups, with no significant difference between the groups studied (p >0.05). The plasma concentration of thiobarbituric acid reactive substances was significantly higher in patients with obesity compared to the control group (p <0.001). There was no correlation between levels of magnesium biomarkers and the concentration of TBARS (p >0.05). Conclusion: Patients with obesity showed a reduced dietary magnesium intake which seems to induce hypomagnesuria as a compensatory mechanism. The marker of oxidative stress evaluated in this study was not influenced by magnesium.

scholarly journals Antioxidant Activity of the Isoflavonoids from the Roots of Maackia amurensis

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Nadezda I. Kulesh ◽  
Sergey A. Fedoreyev ◽  
Marina V. Veselova ◽  
Natalia P. Mischenko ◽  
Vladimir A. Denisenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Glutathione Peroxidase ◽  
Column Chromatography ◽  
Thiobarbituric Acid ◽  
Formalin Injection ◽  
Thiobarbituric Acid Reactive Substances ◽  
H Nmr ◽  
Maackia Amurensis

Seven isoflavonoids, including a new glycoside, (6a R,11a R)-medicarpin-3- O-gentiobioside (6), were isolated from the roots of Maackia amurensis using repeated column chromatography on a Toyopearl HW-50F sorbent and identified by HPLC–PDA–MS, 1H NMR, 13C, 1H–1H COSY, HSQC NMR and HMBC NMR analyses as daidzin (1), genistein-7- O-gentiobioside (2), pseudobaptigenin-7- O-gentiobioside (3), formononetin-7- O-gentiobioside (4), (6a R,11a R)-maackiain-3- O-gentiobioside (5), and 5- O-methylgenistein-7- O-gentiobioside (7). In the model of oxidative stress induced by formalin injection, the isolated isoflavone and pterocarpan glucosides 1-7 were shown to reduce the formation of malondialdehyde (MDA) and other thiobarbituric acid reactive substances (TBARS), as well as glutathione peroxidase (GPO) activity in rats.

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