scholarly journals Pathogenic Bacterial Species in Locally Prepared Fresh Fruit Juices Sold in Juice Houses of Eastern Ethiopia

2021 ◽  
Vol 14 ◽  
pp. 117863612110607
Author(s):  
Dechasa Adare Mengistu ◽  
Negga Baraki ◽  
Tesfaye Gobena Tesema
Keyword(s):  
Pathogenic Bacteria ◽  
Fruit Juice ◽  
Statistical Tests ◽  
Statistical Significance ◽  
Bacterial Species ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Plate Count ◽  
Eastern Ethiopia ◽  
Food Borne

Fruit juices are important components of a healthy diet and a dietary source of nutrients, vitamins, and fiber and vital for human health. However, unless it is handled with safety and hygienic conditions, food can be a vehicle for the transmission of various agents of diseases resulting in food borne outbreaks. Thus, this study aimed to determine common pathogenic bacterial species in locally prepared fresh fruit juices sold in juice houses of Eastern Ethiopia. A cross-sectional study design was conducted from 1 January to 27 March 2020, in eastern Ethiopia. Seventy-eight juice samples were collected aseptically using a sterilized collecting jar from each juice house. Pour plate count method was used to determine Staphylococcus, Salmonella, and Shigella species. Finally, the data were analyzed using descriptive statistical tests such as Chi-square and Fisher’s exact tests. P-value of .05 was considered as a cut point for statistical significance. The study found Staphylococcus count ranged from 1.68 log CFU/mL with the mean value of 4.204 log CFU/mL. Overall, 58 (74.4%) of the fruit juice samples had Staphylococcus count, 19 (24.4%) had Salmonella and 12 (15.4%) had Shigella higher than the maximum permitted limit of Gulf standard, 2000 set for any type of fruit juice. In general, the study found more than two-thirds of fruit juice samples had at least 1 pathogenic bacteria species higher than the maximum permitted limit and potentially hazardous to consumer health. Thus, regular supervision and application of food hygiene and safety are essential to improve the quality of fruit juice and to prevent the consumption of contaminated fruit juices, which leads to food borne illness.

scholarly journals Estimation of Microbial Contamination in Fresh and Commercial Fruit Juices: A Comparative Study

2021 ◽  
pp. 145-153
Author(s):  
Vanessa James ◽  
Hiral Panchal
Keyword(s):  
Staphylococcus Aureus ◽  
Microbial Contamination ◽  
Fruit Juice ◽  
Aloe Vera ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Plate Count ◽  
Human Consumption ◽  
Coliform Count ◽  
Total Plate Count

Aim and Objective: The objective of the present study is to determine microbial contamination in fresh and packaged commercial fruit juices (including a combination of Aloe vera with fruit juices) available in the Ahmedabad city of Gujarat, India. Materials and Methods: Seventeen samples were collected from various parts of the city which includes 9 commercial fruit juice samples and 8 street vended fresh fruit juice samples. Samples were examined for Total plate count, Yeast and mould count, coliform count, Escherichia coli, Salmonella, Staphylococcus aureus, Shigella, Enterobacteriaceae, Listeria monocytogens and Vibrio Cholerae. Results: Commercial fruit juices do not exceed the FSSAI standards for fruit juices and are free of harmful pathogens making themsafe for human consumption. Street vended fresh fruit juice samples exceedthe FSSAI limit for Total Plate count, Yeast and mould count and Coliform count. Street vended Fresh fruit juices demonstratethepresence of Ecoli, Salmonella and Staphylococcus aureus in 75% (6/8) samples. Enterobacteriaceae were identified in street vended fruit juices which exceeds the FSSAI standard limit. Conclusion: The study demonstrates that commercial fruit juices were safe for human consumption but fresh juices showed significant microbial growth and harmful pathogens which must be controlled to ensure consumer’s safety and health. However regular monitoring of commercial and fresh fruit juices is recommended to avoid food borne illness resulting from pathogens encountered in the study.

scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRESH AND FREEZE-DRIED SELECTED FRUIT JUICES

2017 ◽  
Vol 10 (9) ◽  
pp. 156 ◽  
Author(s):  
Naritsara Toobpeng ◽  
Pannapa Powthong ◽  
Pattra Suntornthiticharoen
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Total Phenolic ◽  
Zone Of Inhibition ◽  
Schleichera Oleosa ◽  
Freeze Dried

  Objective: The objective of this investigation was to assess the biological activity of fresh fruit juices and freeze dried fruit juices including antioxidant and antibacterial activity.Methods: The fruits used in this study were Schleichera oleosa (Lour.) Oken, Carissa carandas L., and Sandoricum koetjape (Burm.f.) Merr. The fresh juice sample (FJS) was extracted and also prepared for freeze drying sample (FDS) by freeze dryer. The antioxidant capacity of FJS and FDS was assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays and determination for total phenolic contents (TPCs) by Folin–Ciocalteu’s reagent. Antimicrobial tests were carried out by agar diffusion methods and evaluated by measuring the zone of inhibition against 10 isolates of pathogenic bacteria.Results: The highest antioxidant activity of FJS and FDS was found in S. oleosa and related to the scavenging effect on DPPH radical of FJS (100% concentration) and FDS (200 mg/ml) was found in the value of 93.05±0.31% and 82.18±0.33%, respectively. TPC of FJS and FDS from S. oleosa at the same concentration as in the DPPH assay was 1,003.53±1.96 ug GAE/300 g of matter and 827.77±23.15 ug GAE/100 ml of matter, respectively. Both FJS and FDS revealed antibacterial activity. The zone of inhibition of FJS was ranging from 8.3±0.6 to 24.0±1.0 mm and FDS values of 7.3±0.5-21.0±0.9 mm. The most potent sample against bacteria was FJS and FDS from S. oleosa which FJSs against Escherichia coli ESBL and FDS against Pseudomonas aeruginosa MDR2.Conclusion: Fruit juices of S. oleosa, C. carandas, and S. koetjape associated with antioxidant activity in the form of both fresh fruit juices and FDS. The result of this study showed that the value of DPPH and TPCs were slightly decreased in FDS compare with fresh juice. Screening of FJS and FDS of C. carandas revealed broad spectrum antibacterial activity. However, FJS of S. oleosa and S. koetjape showed inhibition of growth of bacteria, but few of FDS from these fruits could inhibit limited bacterial isolates. For antibacterial activity against pathogenic bacteria, the samples of fresh and freeze dried exhibited a different degree of activity.

scholarly journals Copper-Silver Alloy Coated Door Handles as a Potential Antibacterial Strategy in Clinical Settings

Coatings ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 790
Author(s):  
Nicole Ciacotich ◽  
Lasse Kvich ◽  
Nicholas Sanford ◽  
Joseph Wolcott ◽  
Thomas Bjarnsholt ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Wound Care ◽  
Care Center ◽  
Bacterial Species ◽  
Alloy Coating ◽  
Field Trials ◽  
Plate Count ◽  
Clinical Settings ◽  
Antibacterial Efficacy ◽  
Silver Alloy

Coating surfaces with a copper-silver alloy in clinical settings can be an alternative or complementary antibacterial strategy to other existing technologies and disinfection interventions. A newly developed copper-silver alloy coating has a high antibacterial efficacy against common pathogenic bacteria in laboratory setups, and the purpose of this study is to determine the antibacterial efficacy of this copper-silvery alloy in real-world clinical settings. Two field trials were carried out at a private clinic and a wound care center. Door handles coated with the copper-silver alloy had a lower total aerobic plate count (1.3 ± 0.4 Log CFU/cm2 and 0.8 ± 0.3 Log CFU/cm2, CFU stands for Colony Forming Units) than the reference uncoated material on-site (2.4 ± 0.4 Log CFU/cm2 for the stainless steel and 1.7 ± 0.4 Log CFU/cm2 for the satin brass). The copper-silver alloy did not selectively reduce specific bacterial species. This study points to the possibility of a successful long-term implementation of the copper-silver alloy coating as an antibacterial strategy.

Plaque and salivary pH changes after consumption of fresh fruit juices

2006 ◽  
Vol 30 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Lata Kiran Banan ◽  
Amitha Hegde
Keyword(s):  
Healthy Eating ◽  
Room Temperature ◽  
Fruit Juice ◽  
Dietary Habits ◽  
Grape Juice ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Pineapple Juice ◽  
Concept Of Health ◽  
Salivary Ph

The concept of health has prevailed for centuries and the dietary habits are apparently changing with modernization. "Healthy eating" is now perceived to be important. The desirability of a healthful lifestyle has led to an increased consumption of juices. Drinking large amount of fruit juice is frequently practiced these days and the consumption of these juices is further modified with behavioral habits such as swishing and frothing the drinks around the mouth, sucking frozen fruit juices, use of feeder cups at bed time etc. Hence this study was conducted to find the acidogenic potential of the commonly consumed fresh fruit juices (Grapes, orange, and pineapple) and the juices stored at various temperatures (room temperature, refrigerator and freezer) on the plaque and saliva at various intervals. It was observed that grape juice was more acidogenic compared to orange and pineapple juice. Frozen fruit juices caused a greater drop in plaque and salivary pH followed by the refrigerated juice.

scholarly journals Microbial contamination of raw meat and its environment in retail shops in Karachi, Pakistan

2010 ◽  
Vol 4 (06) ◽  
pp. 382-388 ◽  
Author(s):  
Nafisa Hassan Ali ◽  
Amber Farooqui ◽  
Adnan Khan ◽  
Ameera Yahya Khan ◽  
Shahana Urooj Kazmi
Keyword(s):  
Staphylococcus Aureus ◽  
Environmental Samples ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Raw Meat ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Shigella Species ◽  
Wide Range ◽  
Meat Samples

Background: This study was conducted to examine the frequency of contamination in retail meat available in Karachi, Pakistan. Methodology: Raw meat samples (250) and surface swabs (90) from meat processing equipment and the surrounding environment were analyzed for microbiological contamination. Results: Out of 340 samples, 84% were found to be contaminated with bacterial species, including Klebsiella, Enterobacter, Staphylococcus aureus and Bacillus subtilis. A total of 550 (66%) of the bacterial isolates were potential pathogens. Of these, 342 and 208 isolates were from meat and environmental samples respectively. Food-borne pathogens isolated from meat samples included Escherichia coli O157:H7, Listeria, Salmonella Enteritidis and Shigella species whereas environmental samples yielded Staphylococcus aureus and Shigella species. Four strains of Brucella species were also isolated from meat samples. Total aerobic counts ranged between 108 -1010 CFU/g or cm2. Resistance to a wide range of antibiotics was observed. Resistance rates to ampicillin, amoxicillin, novobiocin and cefaclor were from 62 to 75% in general. Thirty-three percent of Salmonella isolates were resistant to ampicillin.  No quinolone resistance was observed. Biofilm formation was observed among 88 (16%) pathogenic bacteria including E. coli, Klebsiella, Enterobacter species and Staphylococcus aureus. Conclusions: Food-borne pathogens found in retail shops could be sources for horizontal contamination of meat. Our data confirm the circulation of antibiotic resistant and biofilm forming pathogens in raw meat and its environment in retail shops in Pakistan, which could play a role in the spread of antimicrobial resistance amongst food-borne bacteria.

scholarly journals Microbiological quality assessment of locally vended and commercially packed fruit juices in Chittagong city of Bangladesh

2019 ◽  
Vol 27 ◽  
pp. 43-58 ◽  
Author(s):  
F Sultana ◽  
LW Marzan ◽  
SA Mina
Keyword(s):  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Fruit Juice ◽  
Sugar Content ◽  
Fruit Juices ◽  
Coliform Count ◽  
Pathogenic Vibrio ◽  
And Staphylococcus Aureus

Microbiological investigation has been undertaken for freshly prepared and commercially packed fruit juices of Chittagong city, Bangladesh. Physicochemical analysis as pH, sugar content, total soluble solid (TSS), total dissolved solid (TDS), acidity, moisture content and microbiological parameters i.e. total viable count (TVC), total coliform count (TCC), fecal coliform count (FCC), total staphylococcal count (TSC), Vibrio spp., Salmonella spp., Shigella spp. were examined for all samples. No microorganisms were found positive in packed fruit juice samples, whereas the range of TVC, TCC, and TSC were found to be 2.0 × 103 - 1.0 × 105, 0.15 × 102 - 11 × 102 and 1.0 × 102 - 2.0 × 104 cfu/ml respectively, though there was no detectable FCC and Salmonella, Shigella for vended samples. Human pathogenic Vibrio cholerae, Klebsiella pneumoniae and Staphylococcus aureus were identified by biochemical tests and further confirmed by 16S rDNA sequence analysis. Antibiotic sensitivity of Klebsiella pneumoniae and Staphylococcus aureus were found against ciprofloxacin, imipenem, gentamicin, levofloxacin except amoxicillin; whereas Vibrio cholerae showed resistant to all above antibiotics. Hence, proper awareness and regular monitoring are required during vended fruit juice handling as antibiotic resistant pathogenic bacteria can easily spread through the contaminated fruit juices to the consumers. J. bio-sci. 27: 43-58, 2019

scholarly journals Multiplexed Bead-Based Mesofluidic System for Detection of Food-Borne Pathogenic Bacteria

2009 ◽  
Vol 75 (21) ◽  
pp. 6647-6654 ◽  
Author(s):  
Sheng-Quan Jin ◽  
Bin-Cheng Yin ◽  
Bang-Ce Ye
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Microarray Scanner ◽  
Food Borne ◽  
Pcr Products ◽  
Meat Samples ◽  
Multiple Pathogens ◽  
Modified Oligonucleotide

ABSTRACT In the present study, a simple and rapid multiplexed bead-based mesofluidic system (BMS) was developed for simultaneous detection of food-borne pathogenic bacteria, including Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella, Enterobacter sakazakii, Shigella, Escherichia coli O157:H7, and Campylobacter jejuni. This system is based on utilization of isothiocyanate-modified microbeads that are 250 μm in diameter, which were immobilized with specific amino-modified oligonucleotide probes and placed in polydimethylsiloxane microchannels. PCR products from the pathogens studied were pumped into microchannels to hybridize with the oligonucleotide-modified beads, and hybridization signals were detected using a conventional microarray scanner. The short sequences of nucleic acids (21 bases) and PCR products characteristic of bacterial pathogens could be detected at concentrations of 1 pM and 10 nM, respectively. The detection procedure could be performed in less than 30 min with high sensitivity and specificity. The assay was simple and fast, and the limits of quantification were in the range from 500 to 6,000 CFU/ml for the bacterial species studied. The feasibility of identification of food-borne bacteria was investigated with samples contaminated with bacteria, including milk, egg, and meat samples. The results demonstrated that the BMS method can be used for effective detection of multiple pathogens in different foodstuffs.

scholarly journals Bioavailability of carotenoids and α-tocopherol from fruit juices in the presence of absorption modifiers:in vitroandin vivoassessment

2008 ◽  
Vol 101 (4) ◽  
pp. 576-582 ◽  
Author(s):  
F. Granado-Lorencio ◽  
C. Herrero-Barbudo ◽  
I. Blanco-Navarro ◽  
B. Pérez-Sacristán ◽  
B. Olmedilla-Alonso
Keyword(s):  
Fruit Juice ◽  
Statistical Significance ◽  
Fruit Juices ◽  
Related Factors ◽  
Micellar Phase ◽  
Factors Affecting ◽  
Supplementation Period ◽  
Before And After

The food industry is playing an increasing role in the development and marketing of new products although little is known regarding the bioavailability of the phytochemicals they contain. Our aim was to assess the effect of the presence of absorption modifiers (milk and iron) on thein vitrobioaccessibility and the serum responsein vivoof carotenoids and α-tocopherol from fruit juices. Thirty-two young women participated in a three-period (21 d each) supplementation study with a 2-week wash-out in between. Subjects consumed consecutively 2 × 250 ml/d vitamin C-fortified juices supplied as fruit juice, fruit juice containing milk and fruit juice containing milk and iron. Fasting blood samples were collected before and after each supplementation period.In vitrobioaccessibility of carotenoids and α-tocopherol was assessed by a static digestion model. Vitamin E and carotenoids from both studies were determined by HPLC.In vitro, xanthophyll ester hydrolysis and transference of free xanthophylls and α-tocopherol into the micellar phase were higher in the presence of absorption modifiers.In vivo, consumption of the fruit juices provoked significant increments (within-subject) of α-tocopherol and some carotenoids in serum. Dose-adjusted increments in serum of some carotenoids were higher when subjects consumed juices with milk and milk plus iron, although differences did not reach statistical significance. In conclusion, the presence of milk and milk plus iron do not influence the bioavailability of carotenoids and α-tocopherol from fruit juicesin vivo. Our results support the use ofin vitromodels to assess food-related factors affecting bioavailability of carotenoids and tocopherols from foods.

scholarly journals Biopreservative Potential of the Spices; Piper Guineense (Uziza), Xylopia Aethiopica (Uda) and Tetrapleura Tetraptera (Oshorisho) in Fresh Fruit Juices

2020 ◽  
pp. 1-6
Author(s):  
Anumudu C K ◽  
◽  
Okolo C A ◽  
Ezembu E N ◽  
◽  
...  
Keyword(s):  
Fruit Juice ◽  
Bacterial Load ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Food Preservatives ◽  
Piper Guineense ◽  
Pineapple Juice ◽  
Tetrapleura Tetraptera ◽  
Fungal Load ◽  
Xylopia Aethiopica

This study demonstrates the antimicrobial activity of three spices, Piper guineense (Uziza), Xylopia aethiopica (Uda), Tetrapleura tetraptera (oshorisho) and their potential use as food biopreservatives. Essential oils of the spices seed were obtained and used in the agar-diffusion and tube dilution assays to determine their minimum inhibition (MIC) and bactericidal concentrations (MBC) against a common fruit juice spoilage organism (Bacillus cereus). MIC values of 50mg/ ml (Oshorisho) and 25mg/ml (Uziza and Uda) were obtained. Similarly, all spice extracts were bactericidal at a concentration of 100mg/ml. The spice extracts were utilized in the fortification of fresh fruit juice. Fortification of orange, pineapple and watermelon juices with extracts of the spices caused a reduction in the bacterial and fungi load of the juices over a period of 7 days in comparison to unpreserved controls. Orange juice preserved with uda had a fungal load of 1.9 x106 and bacterial load of 8.0 x106 compared to control with loads of 2.3 x107 and 1.1 x107 , respectively. Watermelon juice preserved with oshorisho had a fungal load of 1.2 x106 and bacterial load of 1.1 x107 compared to control with loads of 2.1 x107 and 8.8 x107 , respectively while pineapple juice preserved with uziza had a fungal load of 1.2 x107 and bacterial load of 1.6 x107 compared to control with loads of 2.7 x107 and 2.5 x108 , respectively. These results show that the spice extracts have potential for use as food preservatives for extending the shelf life of freshly prepared fruit juices. This is important as the demand for fresh foods free from chemical preservatives but microbiologically safe is on the increase.

scholarly journals Isolation of potential bacterial pathogens from the phylloplane of some selected medicinal plants

2016 ◽  
Vol 4 (2) ◽  
pp. 37
Author(s):  
Haruna Yahaya ismail ◽  
Hauwa S. Bello ◽  
Ibrahim A. Allamin ◽  
Elizabeth Danjuma
Keyword(s):  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Mangifera Indica ◽  
Statistical Significance ◽  
Bacterial Species ◽  
Salmonella Typhi ◽  
Hot Water ◽  
Mango Leaves ◽  
Guava Leaves ◽  
Prevalent Species

Microorganisms are ubiquitous and their impact could be appreciated directly or indirectly. This study was aimed at determining the occurrence of pathogenic bacteria on the phylloplane of some medicinal plants commonly used to treat diseases by oral administration. Leaves samples were collected aseptically from Cassia fistula (Cassia) Mangifera indica (Mango) and Psidium guajavum (Guava) and their bacterial contents were assessed. Higher bacterial counts were observed in raw samples followed by those washed with sterile water. Samples washed with sterile warm water (blanched) were shown to have lower counts although without statistical significance (p ≤ 0.05). Guava leaves had higher counts (3.4×106 cfu/g) in the raw samples and cassia in blanched (6.4×105 cfu/g). However, mango leaves had the lowest (8.4×105 cfu/g and 2.0×105 cfu/g) in both the treatments respectively. Twenty bacterial species were isolated comprising Gram negative and Gram positive species. Bacterial identification revealed that Proteus mirabilis (35%), Proteus vulgaris (15%), Escherichia coli (15%), Klebsiella pneumoniae (5%), Morganella morganii (5%), Salmonella typhi (10%), Enterobacter sp. (5%), Staphylococcus aureus (5%) and Vibrio cholarae (5%) were the prevalent species. These organisms could be potential pathogens and proper washing with hot water may serve a better means of reducing the microbial contents and thus, it is recommended.

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