scholarly journals A Cytotoxic Diterpenoid from Croton Membranaceus, the Major Constituent of Anticancer Herbal Formulations Used in Ghana

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301 ◽  
Author(s):  
Marcel T. Bayor ◽  
John S. K. Ayim ◽  
Gemma Marston ◽  
Roger M. Phillips ◽  
Steven D. Shnyder ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Traditional Healers ◽  
Major Constituent ◽  
Cytotoxic Activities ◽  
Traditional Use ◽  
Bioassay Guided Fractionation ◽  
Prostate Cancers ◽  
Herbal Formulations

Croton membranaceus is used by herbalists and traditional healers in Ghana for the management of various cancers, especially prostate cancers. A methanolic extract of the roots showed cytotoxic activities against two cancer cell lines, and bioassay-guided fractionation of this extract revealed that the cytotoxic activity resided mostly in the ethyl acetate fraction. Six compounds were isolated from this fraction, including a new furano-clerodane diterpenoid (1), for which the trivial name crotomembranafuran is suggested. This compound exhibited an IC50 value of 4.1 μg/mL (10.6 μM) against human prostate (PC-3) cells, providing some support for the traditional use of C. membranaceus in the treatment of cancers.

Secondary Metabolites of Ponderosa Lemon (Citrus pyriformis) and their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities

2011 ◽  
Vol 66 (7-8) ◽  
pp. 385-393 ◽  
Author(s):  
Dalia Hamdan ◽  
Mahmoud Zaki El-Readi ◽  
Ahmad Tahrani ◽  
Florian Herrmann ◽  
Dorothea Kaufmann ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
2D Nmr ◽  
Positive Control ◽  
Cytotoxic Activities ◽  
Fruit Peel ◽  
Ether Fraction ◽  
Anti Inflammatory

Column chromatography of the dichloromethane fraction from an aqueous methanolic extract of fruit peel of Citrus pyriformis Hassk. (Rutaceae) resulted in the isolation of seven compounds including one coumarin (citropten), two limonoids (limonin and deacetylnomilin), and four sterols (stigmasterol, ergosterol, sitosteryl-3-β-D-glucoside, and sitosteryl-6ʹ- O-acyl-3-β-D-glucoside). From the ethyl acetate fraction naringin, hesperidin, and neohesperidin were isolated. The dichloromethane extract of the defatted seeds contained three additional compounds, nomilin, ichangin, and cholesterol. The isolated compounds were identified by MS (EI, CI, and ESI), 1H, 13C, and 2D-NMR spectral data. The limonoids were determined qualitatively by LC-ESI/MS resulting in the identification of 11 limonoid aglycones. The total methanolic extract of the peel and the petroleum ether, dichloromethane, and ethyl acetate fractions were screened for their antioxidant and anti-inflammatory activities. The ethyl acetate fraction exhibited a significant scavenging activity for DPPH· free radicals (IC50 = 132.3 μg/mL). The petroleum ether fraction inhibited 5-lipoxygenase with IC50 = 30.6 μg/mL indicating potential anti-inflammatory properties. Limonin has a potent cytotoxic effect against COS7 cells [IC50 = (35.0 ± 6.1) μM] compared with acteoside as a positive control [IC50 = (144.5 ± 10.96) μM]

Antioxidant and Anti-inflammatory Activities of Organic and Aqueous Extracts of Northeast Algerian Marrubium vulgare

2018 ◽  
Vol 16 (S1) ◽  
pp. S119-S129
Author(s):  
I. Namoune ◽  
B. Khettal ◽  
A.M. Assaf ◽  
S. Elhayek ◽  
L. Arrar
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Ethyl Acetate Extract ◽  
Interleukin 1 ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Traditional Use ◽  
Marrubium Vulgare ◽  
Anti Inflammatory

Marrubium vulgare (Lamiaceae) is frequently used in traditional medicine to treat many illnesses from ancient times. Its beneficial effects include antibacterial, antioedematogenic, and analgesic activities. This study was designed to evaluate the antioxidant and anti-inflammatory activities of organic and aqueous extracts of the leaves, the flowers, the stems, and the roots of Marrubium vulgare. The total phenolic and flavonoid contents as well as the antioxidant and the anti-inflammatory effects of methanol, chloroform, ethyl acetate, and aqueous extracts have been investigated by using different in-vitro methods. It was found that the ethyl acetate extract from Marrubium vulgare stems had the highest total phenolic content, while the ethyl acetate extract from the leaves yielded a high concentration of flavonoids. The ethyl acetate extract from the stems exhibited the highest activity in scavenging of 2,2-diphenyl- 1-picrylhydrazyl (DPPH), as well as in protecting erythrocytes. The leaves aqueous extract exhibited the highest ferrous chelating activity and its methanolic extract was found to be the strongest inhibitor of lipid peroxidation in β-carotene bleaching assay. The leaves chloroform extracts as well as the flowers methanol, chloroform, and ethyl acetate extracts were found to decrease the pro-inflammatory tumor necrosis factor alpha (TNF-α) cytokine levels in a dose-dependent manner. On the other hand, the flowers methanolic extract and the leaves methanol, ethyl acetate, and aqueous extracts decreased the interleukin-1 beta (IL- 1β) release. It was also found that the methanol extract from the flowers and the chloroform extract from the stems of Marrubium vulgare inhibited interleukin-8 (IL-8) release. This study provides a scientific basis for the traditional use of Marrubium vulgare as an anti-inflammatory agent and for the plant to be considered as an important resource of natural antioxidants.

scholarly journals Marine Natural Products, IX A New Halogenated Sesquiterpene Pinnatifidone from the Red Alga Laurencia pinnatifida

1988 ◽  
Vol 43 (10) ◽  
pp. 1347-1350 ◽  
Author(s):  
Shaheen Bano ◽  
Mohammad Shaiq Ali ◽  
Viqar Uddin Ahmad
Keyword(s):  
Natural Products ◽  
Ethyl Acetate ◽  
Marine Natural Products ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Spectroscopic Studies ◽  
Red Alga

Abstract From the ethyl acetate fraction of methanolic extract of red alga, L.pinnatifida, a new halogenated sesquiterpene named as pinnatifidone [1] has been isolated and the structure of this compound has been elucidated with the help of intensive spectroscopic studies.

scholarly journals The ethyl acetate fraction of a methanolic extract of unripe noni (Morinda citrifolia Linn.) fruit exhibits a biphasic effect on the dopaminergic system in mice

2017 ◽  
Vol 66 (3) ◽  
pp. 283-291 ◽  
Author(s):  
Vijayapandi Pandy ◽  
Megala Narasingam ◽  
Kamini Vijeepallam ◽  
Syam Mohan ◽  
Vasudevan Mani ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Dopaminergic System ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Morinda Citrifolia ◽  
Biphasic Effect

Exploring cytotoxic and antioxidant properties of Heliotropium calcareum in polar and non-polar extracts

2021 ◽  
Vol 02 ◽  
Author(s):  
Mohammad Uzair ◽  
Faisal Rashid ◽  
Hamid Saeed Shah ◽  
Jamshed Iqbal
Keyword(s):  
Cell Cycle ◽  
Ethyl Acetate ◽  
Hela Cells ◽  
Biological Activities ◽  
Research Work ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Cell Cycle Analysis ◽  
G1 Phase ◽  
Traditional Use

Background: Plants are a vital source of natural drugs as the traditional use of plants as therapeutic agents for a variety of ailments has been traced back to thousands of years. The utilization of Heliotropium calcareum has been evident since ancient times for treating various disease states like inflammation associated with gout and rheumatism, poisonous bites, and other skin disorders. The current research work was carried out to determine the phytochemistry and biological activities of the crude methanolic extract obtained through maceration from the aerial parts of Heliotropium calcareum. Methods: The plant was collected from district Bhakkar, Punjab, Pakistan. Maximum phenolic (74.5 µg GAE/mg) and flavonoid content (58.99 µg QE/mg) were observed in ethyl acetate fraction. Significant antioxidant potential was observed in ethyl acetate fraction with the highest free radical hunting activity of 92.6 ± 6.7 µM. Results: Cytotoxicity assay using MTT dye was performed where non-polar (n-hexane) and polar (ethyl acetate) fractions displayed excellent cytotoxicity against HeLa cells (IC50 = 79.95 ± 3.718 & 164 ± 4 µg/mL respectively). Furthermore, the above fractions showed momentous results in cell cycle analysis and promising proapoptotic effect against cervical (HeLa) cancer cell lines. An n-hexane and ethyl acetate fraction were selected for cell cycle analysis to determine the quantitative measurement of the degree of apoptosis. According to the results given below in the figure, the cervical (HeLa) cancer cells were treated with n-hexane and ethyl acetate fractions at various concentrations. An increase in the cell population at G0/G1 phase and a decrease in the S-phase population concerning untreated cells suggested the G0/G1 phase arrest in n-hexane and ethyl acetate fractions treated HeLa cells. Conclusion: Overall, , n-hexane and ethyl acetate fractions were found to be the most promising and active elements of H. calcareum and may be utilized to explore their cytotoxic effects further in the animal model.

scholarly journals Methanolic extract of Potentilla fulgens root and its ethyl-acetate fraction delays the process of carcinogenesis in mice

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Buddha Ganguly ◽  
Alka Chaudhary ◽  
Hughbert Dakhar ◽  
Inder Pal Singh ◽  
Anupam Chatterjee
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Genetic Alterations ◽  
Gastric Carcinogenesis ◽  
Check Point ◽  
Over Expression ◽  
North Eastern ◽  
Ad Libitum

AbstractPeople of north-eastern states of India consume raw areca-nut (RAN) and lime which could lead to oral, esophageal and gastric cancers. However, the incidence of these cancers are significantly lesser in those who consume pieces of Potentilla fulgens root along with RAN. Since evaluation of anticancer role, if any, of P. fulgens on RAN-mediated genetic alterations in human is difficult because of other compounding factors, this study was undertaken in mice to focus on gastric carcinogenesis since ad libitum administration of RAN extract with lime in drinking water induced stomach cancer due to greater exposure of its lining. A total of 160 mice were used at different time points and either methanol extract of P. fulgens roots (PRE) or mixture of four compounds of ethyl-acetate fraction (EA-mixture) was mixed with mice feed. Histological studies revealed that RAN + lime induced cancer in all the mice and interestingly only 20% developed cancer when PRE/EA-mixture was provided along with RAN + lime. Higher frequency of precocious anaphase and over expression of p53 and Securin genes were significantly reduced by PRE/EA-mixture. Thus PRE/EA-mixture mitigates the RAN-induced tumor-initiating process in stomach by maintaining expression of tumor suppressor and check-point genes under control.

scholarly journals Cytotoxic Alkaloids from Leaves and Twigs of Dasymaschalon sootepense

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Sakchai Hongthong ◽  
Chutima Kuhakarn ◽  
Vichai Reutrakul ◽  
Surawat Jariyawat ◽  
Pawinee Piyachaturawat ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Cancer Cell Lines ◽  
Spectroscopic Methods ◽  
Aporphine Alkaloid ◽  
First Report ◽  
Bioassay Guided Fractionation

Bioassay-guided fractionation of the cytotoxic ethyl acetate fraction of the sequential methanol extract from the leaves and twigs of Dasymaschalon sootepense led to the isolation of a new 7-hydroxy aporphine alkaloid, 6a,7-dehydrodasymachaline (1) along with the five known compounds (-)-nordicentrine (2), dicentrinone (3), (-)-sinactine (4), aristolactam AII (5) and epiberberine (6). Their structures were elucidated by spectroscopic methods. This is the first report of alkaloids 1–2 and 5–6 from the genus Dasymaschalon. Compounds 1 and 5 showed cytotoxicity against a panel of cancer cell lines.

scholarly journals In VitroandIn VivoAntimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Farzaneh Naghibi ◽  
Somayeh Esmaeili ◽  
Noor Rain Abdullah ◽  
Mehdi Nateghpour ◽  
Mahdieh Taghvai ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Methanolic Extract ◽  
Cytotoxic Activities ◽  
Traditional Use ◽  
Aerial Parts ◽  
Mammalian Cell Lines ◽  
Phytochemical Investigation

Based on the collected ethnobotanical data from the Traditional Medicine and Materia Medica Research Center (TMRC), Iran,Myrtus communisL. (myrtle) was selected for the assessment ofin vitroandin vivoantimalarial and cytotoxic activities. Methanolic extract of myrtle was prepared from the aerial parts and assessed for antiplasmodial activity, using the parasite lactate dehydrogenase (pLDH) assay against chloroquine-resistant (K1) and chloroquine-sensitive (3D7) strains ofPlasmodium falciparum. The 4-day suppressive test was employed to determine the parasitemia suppression of the myrtle extract againstP. berghei  in vivo. The IC50values of myrtle extract were 35.44 µg/ml against K1 and 0.87 µg/ml against 3D7. Myrtle extract showed a significant suppression of parasitaemia (84.8 ± 1.1% at 10 mg/kg/day) in mice infected withP. bergheiafter 4 days of treatment. Cytotoxic activity was carried out against mammalian cell lines using methyl thiazol tetrazolium (MTT) assay. No cytotoxic effect on mammalian cell lines up to 100 µg/mL was shown. The results support the traditional use of myrtle in malaria. Phytochemical investigation and understanding the mechanism of action would be in our upcoming project.

Evaluation of Cytotoxic Compounds from Calligonum comosum L. Growing in Egypt

2007 ◽  
Vol 62 (9-10) ◽  
pp. 656-660 ◽  
Author(s):  
Farid A. Badria ◽  
Madiha Ameen ◽  
Mohamed R. Akl
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methylene Chloride ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Desert Plant ◽  
Ehrlich Ascites ◽  
Cytotoxic Compounds ◽  
Egyptian Desert

Calligonum comosum (Polygonaceae), an Egyptian desert plant, was extracted and fractionated using petroleum ether, methylene chloride, and ethyl acetate. The total methanolic extract and other fractions were tested for their anticancer activity using Ehrlich ascites, brine shrimp and antioxidant assays. Ethyl acetate fraction proved to be the most active in all assays. Eight compounds were isolated, purified, and identified from this fraction as (+)- catechin (1), dehydrodicatechin A (2), kaempferol-3-O-rhamnopyranoside (3), quercitrin (quercetin-3-O-rhamnopyranoside) (4), β-sitosterol-3-O-glucoside (5), isoquercitrin (quercetin- 3-O-glucopyranoside) (6), kaempferol-3-O-glucuronide (7), and mequilianin (quercetin-3- O-glucuronide) (8). All isolated compounds were tested for their cytotoxicity and antioxidant activity. Compound 2 showed the best cytotoxic and antioxidant activity.

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