Anti-inflammatory Constituents from the Aerial Parts of Iris minutiaurea

Phytochemical investigation of the methanol extract of the aerial parts of Iris minutiaurea (Iridaceae) using column chromatography led to the isolation of a new xanthone glycoside, 1-hydroxy-3,5-dimethoxy-xanthone-6- O-β-D-glucoside (1), together with one known flavonoid glycoside (2). The structure of this new compound was elucidated by analysis of spectroscopic, including ID (1H, 13C), 2D NMR (COSY, HMQC, HMBC), and high resolution fast atom bombardment mass spectrometric (HR-FAB-MS) data and enzyme hydrolysis. We found that compounds 1 and 2 significantly suppressed production of NO, and pro-inflammatory cytokine in LPS-induced RAW264.7 cells. These results suggest that compound 1 and 2 have anti-inflammatory activity related with production of TNF-α, IL-6, IL-β, and NO in macrophages, and then compound 1 were more efficient than compound 2 in lowering the level of proinflammatory cytokine.

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Phytochemical Profile of the Aerial Parts of Sedum sediforme and Anti-inflammatory Activity of Myricitrin

Natural Product Communications ◽

10.1177/1934578x1501000122 ◽

2015 ◽

Vol 10 (1) ◽

pp. 1934578X1501000 ◽

Cited By ~ 2

Author(s):

Daniel Winekenstädde ◽

Apostolis Angelis ◽

Birgit Waltenberger ◽

Stefan Schwaiger ◽

Job Tchoumtchoua ◽

...

Keyword(s):

Secondary Metabolites ◽

Methanol Extract ◽

2D Nmr ◽

Prostaglandin E ◽

Arachidonic Acid Cascade ◽

Aerial Parts ◽

Phytochemical Profile ◽

Anti Inflammatory ◽

New Compounds ◽

First Time

The aim of this study was to investigate the phytochemical profile of the methanol extract of the aerial parts of Sedum sediforme and to identify its secondary metabolites. By means of chromatographic separation and enrichment of compounds, HPLC-ESI-MS, HRMS, 1D-, 2D- NMR and/or comparison with reference compounds, three triterpenes, two sterols, ten flavonoids and twelve phenolic compounds were identified, together with two new compounds, i.e. (2 R*, 3 R*)-5,7-dihydroxy-2,3-dimethyl-4-chromanone-7- O-ß-D-glucoside (27) and butan-2- O-rutinoside (28). Out of the 29 identified secondary metabolites, 18 are described as ingredients of S. sediforme herein for the first time. Furthermore, myricitrin, one of the major constituents, was tested for its ability to inhibit different enzymes within the arachidonic acid cascade in order to determine its anti-inflammatory properties. Whereas there was only either weak or no inhibition of the microsomal prostaglandin E2 synthase-1 (mPGES-1) and the soluble epoxide hydrolase (sEH), myricitrin showed strong inhibition of 5-lipoxygenase (5-LO), with an IC50 of 7.8 ± 0.2 μM.

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Four New Pterosins from Pteris cretica and Their Cytotoxic Activities

Molecules ◽

10.3390/molecules24152767 ◽

2019 ◽

Vol 24 (15) ◽

pp. 2767

Author(s):

Jian Lu ◽

Caiying Peng ◽

Shuang Cheng ◽

Jianqun Liu ◽

Qinge Ma ◽

...

Keyword(s):

Human Tumor ◽

2D Nmr ◽

Cytotoxic Activities ◽

Aerial Parts ◽

Pteris Cretica ◽

Phytochemical Investigation ◽

Hct 116 ◽

Hct 116 Cells ◽

New Compounds

Phytochemical investigation of the aerial parts of Pteris cretica led to the isolation and elucidation of nine pterosins, including four new pterosins, creticolacton A (1), 13-hydroxy-2(R),3(R)-pterosin L (2), creticoside A (3), and spelosin 3-O-β-d-glucopyranoside (4), together with five known pterosins 5–9. Their structures were identified mainly on the basis of 1D and 2D NMR spectral data, ESI-MS and literature comparisons. Compounds 1 and 3 were new type of petrosins with a six membered ring between C-14 and C-15. The new compounds were tested in vitro for their cytotoxic activities against four human tumor cell lines (SH-SY5Y, SGC-7901, HCT-116, Lovo). Results showed that compounds 1 and 2 exhibited cytotoxic activity against HCT-116 cells with IC50 value of 22.4 μM and 15.8 μM, respectively.

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Isolation and Quantification of Pinitol, a Bioactive Cyclitol, in Retama spp

Natural Product Communications ◽

10.1177/1934578x1601100321 ◽

2016 ◽

Vol 11 (3) ◽

pp. 1934578X1601100 ◽

Cited By ~ 2

Author(s):

Nuria H. González-Mauraza ◽

Antonio J. León-González ◽

José L. Espartero ◽

Juan B. Gallego-Fernández ◽

Marina Sánchez-Hidalgo ◽

...

Keyword(s):

Mediterranean Region ◽

2D Nmr ◽

Spectroscopic Techniques ◽

Aqueous Extracts ◽

Aerial Parts ◽

The Mediterranean ◽

Anti Inflammatory ◽

Identification And Quantification

The genus Retama (Fabaceae) is widely distributed in the Mediterranean region. In the present study, pinitol (3- O-methyl- chiro-inositol), an anti-inflammatory and antidiabetic molecule, was isolated from aerial parts of R. monosperma, and its structure established on the basis of spectroscopic techniques (1D/2D NMR) and MS. Identification and quantification of pinitol in R. raetam and R. sphaerocarpa were also performed. R. monosperma had the highest concentration of pinitol (2.3%). The presence of pinitol in aqueous extracts of Retama spp. may explain the adaptation of these plants to drought and salinity. Furthermore, pinitol could be considered as a mediator in the anti-inflammatory and hypoglycemic activities of Retama spp., which are traditionally used to treat diabetes.

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Analgesic and Anti-Inflammatory Activities of the Methanol Extract ofElaeagnus oldhamiiMaxim. in Mice

The American Journal of Chinese Medicine ◽

10.1142/s0192415x12500449 ◽

2012 ◽

Vol 40 (03) ◽

pp. 581-597 ◽

Cited By ~ 12

Author(s):

Chi-Ren Liao ◽

Yuan-Shiun Chang ◽

Wen-Huang Peng ◽

Shang-Chih Lai ◽

Yu-Ling Ho

Keyword(s):

Acetic Acid ◽

Formalin Test ◽

Methanol Extract ◽

Antioxidant Activities ◽

Late Phase ◽

Paw Edema ◽

Hplc Fingerprint ◽

Tnf Α ◽

Cox 2 ◽

Anti Inflammatory

We investigated possible mechanisms of analgesic and anti-inflammatory activities of the methanol extract from the leaf of Elaeagnus oldhamii Maxim. (EOMeOH). EOMeOHwas evaluated for its analgesic activity in acetic acid-induced writhing response and formalin test, and anti-inflammatory effect was examined by λ-carrageenan-induced paw edema assay. We detected the activities of GPx, GRd and SOD in the liver, and the levels of inflammatory mediators including IL-1β, IL-6, TNF-α, COX-2, MDA and NO in the edema paw to investigate the mechanism of action against inflammation. Total polyphenol, flavonoid and flavanol contents of EOMeOHwere detected to explore its antioxidant activities. Results showed that, in the analgesic test, EOMeOHdecreased acetic acid-induced writhing response and the licking time in the late phase of formalin test. In the anti-inflammatory test, EOMeOHdecreased paw edema at the 2nd, 3rd, 4th and 5th h after λ-carrageenan had been injected. EOMeOHincreased the activities of SOD and GPx in liver tissue and decreased MDA, NO, IL-1β, IL-6, TNF-α and COX-2 levels in paw edema tissue at the 3rd h after λ-carrageenan-induced inflammatory reaction. EOMeOHexhibited abundant polyphenol, flavonoid and flavanol contents. In HPLC fingerprint test of EOMeOH, two index ingredients, ursolic acid and pomolic acid, were isolated from EOMeOHand were exhibited in HPLC chromatographic analysis. The results demonstrated analgesic and anti-inflammatory effects of EOMeOH. It was indicated that the anti-inflammatory mechanism of EOMeOHmay be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx and GRd in the liver. Additionally, EOMeOHdecreased IL-1β, IL-6, TNF-α and COX-2 levels in the edema paw. The results suggested its value in future development of herbal medicine for the treatment of inflammatory diseases.

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Analgesic and Anti-Inflammatory Activities of Methanol Extract ofFicus pumilaL. in Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/340141 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Chi-Ren Liao ◽

Chun-Pin Kao ◽

Wen-Huang Peng ◽

Yuan-Shiun Chang ◽

Shang-Chih Lai ◽

...

Keyword(s):

Acetic Acid ◽

Methanol Extract ◽

Inflammatory Diseases ◽

Paw Edema ◽

Tissue Destruction ◽

Hplc Fingerprint ◽

Analgesic Effects ◽

Inflammatory Mechanism ◽

Tnf Α ◽

Anti Inflammatory

This study investigated possible analgesic and anti-inflammatory mechanisms of the methanol extract ofFicus pumila(FPMeOH). Analgesic effects were evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The results showedFPMeOHdecreased writhing response in the acetic acid assay and licking time in the formalin test. The anti-inflammatory effect was evaluated by λ-carrageenan-induced mouse paw edema and histopathological analyses.FPMeOHsignificantly decreased the volume of paw edema induced by λ-carrageenan. Histopathologically,FPMeOHabated the level of tissue destruction and swelling of the edema paws. This study indicated anti-inflammatory mechanism ofFPMeOHmay be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx, and GRd in the liver. Additionally,FPMeOHalso decreased the level of inflammatory mediators such as IL-1β, TNF-α, and COX-2. HPLC fingerprint was established and the contents of three active ingredients, rutin, luteolin, and apigenin, were quantitatively determined. This study provided evidence for the classical treatment ofFicus pumilain inflammatory diseases.

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Phytochemical Investigation of Tradescantia Albiflora and Anti-Inflammatory Butenolide Derivatives

Molecules ◽

10.3390/molecules24183336 ◽

2019 ◽

Vol 24 (18) ◽

pp. 3336 ◽

Cited By ~ 2

Author(s):

Ping-Chen Tu ◽

Han-Chun Tseng ◽

Yu-Chia Liang ◽

Guan-Jhong Huang ◽

Te-Ling Lu ◽

...

Keyword(s):

2D Nmr ◽

Raw 264.7 Cells ◽

No Production ◽

Isolation And Characterization ◽

Whole Plant ◽

Phytochemical Investigation ◽

Ic50 Value ◽

Anti Inflammatory ◽

New Compounds ◽

First Time

Phytochemical investigation of the whole plant of Tradescantia albiflora Kunth led to the isolation and characterization of a butanolide, rosmarinosin B (1), that was isolated from natural sources for the first time, a new butenolide, 5-O-acetyl bracteanolide A (2), and a new apocarotenoid, 2β-hydroxyisololiolide (11), together with 25 known compounds (compounds 3–10 and 12–28). The structures of the new compounds were elucidated by analysis of their spectroscopic data, including MS, 1D, and 2D NMR experiments, and comparison with literature data of known compounds. Furthermore, four butenolides 4a–4d were synthesized as novel derivatives of bracteanolide A. The isolates and the synthesized derivatives were evaluated for their preliminary anti-inflammatory activity against lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in RAW 264.7 cells. Among them, the synthesized butenolide derivative n-butyl bracteanolide A (4d) showed enhanced NO inhibitory activity compared to the original compound, with an IC50 value of 4.32 ± 0.09 μg/mL.

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The Flavonoid Quercetin Inhibits Proinflammatory Cytokine (Tumor Necrosis Factor Alpha) Gene Expression in Normal Peripheral Blood Mononuclear Cells via Modulation of the NF-κβ System

Clinical and Vaccine Immunology ◽

10.1128/cvi.13.3.319-328.2006 ◽

2006 ◽

Vol 13 (3) ◽

pp. 319-328 ◽

Cited By ~ 201

Author(s):

Madhavan P. Nair ◽

Supriya Mahajan ◽

Jessica L. Reynolds ◽

Ravikumar Aalinkeel ◽

Harikrishnan Nair ◽

...

Keyword(s):

Gene Expression ◽

Proinflammatory Cytokine ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Cytokine Tumor Necrosis Factor ◽

Factor Alpha ◽

Anti Inflammatory ◽

Necrosis Factor

ABSTRACT The flavonoids comprise a large class of low-molecular-weight plant metabolites ubiquitously distributed in food plants. These dietary antioxidants exert significant antitumor, antiallergic, and anti-inflammatory effects. The molecular mechanisms of their biological effects remain to be clearly understood. We investigated the anti-inflammatory potentials of a safe, common dietary flavonoid component, quercetin, for its ability to modulate the production and gene expression of the proinflammatory cytokine tumor necrosis factor alpha (TNF-α) by human peripheral blood mononuclear cells (PBMC). Our results showed that quercetin significantly inhibited TNF-α production and gene expression in a dose-dependent manner. Our results provide direct evidence of the anti-inflammatory effects of quercetin by PBMC, which are mediated by the inhibition of the proinflammatory cytokine TNF-α via modulation of NF-κβ1 and Iκβ.

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NEW AROMATIC ALCOHOL, BENZOYL GLYCOSIDIC ESTER AND ANTHRAQUINONE DERIVATIVE FROM THE AERIAL PARTS OF PHYLLANTHUS FRATERNUS WEBSTER

INDIAN DRUGS ◽

10.53879/id.55.11.11503 ◽

2018 ◽

Vol 55 (11) ◽

pp. 40-44

Author(s):

A Abuzer ◽

◽

M. Jameel ◽

M. Ali

Keyword(s):

Urinary Tract ◽

Kidney Stones ◽

Methanolic Extract ◽

2D Nmr ◽

Anthraquinone Derivative ◽

Aromatic Alcohol ◽

Aerial Parts ◽

Phytochemical Investigation ◽

Ft Ir ◽

Phyllanthus Fraternus

Phyllanthus fraternus Webster (Euphorbiaceae) is used in Indian traditional systems of medicine to treat diabetes, influenza, dyspepsia, indigestion, dysentery, jaundice, kidney stones, urinary tract diseases, vaginitis and skin eruptions. Phytochemical investigation of a methanolic extract of aerial parts of P. fraternus led to the isolation of a new aromatic alcohol characterized as 3'-methyl-3'-(3,4- dimethoxyphenyl)-1'-propanol (1), a benzoyl glycosidic ester identified as 2-n-dodecanoyl-salicyloyl-O- β-D-arabinopyranoside (2) and a new anthraquinone derivative 1-methoxy-2,4,8,9,10,11-hexahydroxy- 3-methyl-6,13-anthracenedione (3) along with a known β-D-diglucoside (4). The structures of isolated phytoconstituents were established on the basis of 1D and 2D NMR, FT-IR, UV and MS data.

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Anti-inflammatory effect of the methanol extract from Anthocephalus cadamba stem bark in animal models

International Journal of Plant Biology ◽

10.4081/pb.2010.e6 ◽

2010 ◽

Vol 1 (1) ◽

pp. 6 ◽

Cited By ~ 6

Author(s):

Kodangala Subraya Chandrashekar ◽

Kodangala Subraya Prasanna ◽

Borthakur Abinash

Keyword(s):

Methanol Extract ◽

Stem Bark ◽

Cellular Migration ◽

Inflammatory Activity ◽

Traditional Use ◽

Aerial Parts ◽

Anti Inflammatory ◽

Design And Methods ◽

Anthocephalus Cadamba ◽

Inflammatory Effect

Background: Anthocephalus cadamba (Rebox)Miq. (Rubiaceae) is widely distributed throughout the greater part of India, especially at low levels in wet place. Traditionally the bark is used as tonic, febrifuge and to reduce the pain and inflammation. The anti-inflammatory effect of methanol extract obtained from Anthocephalus cadamba aerial parts, MEAC, were investigated in this study. Design and methods: The effects of MEAC on the acute and chronic phases of inflammation were studied in carrageenan, dextran and mediators (histamine and serotonin) induced paw edema and cotton pallet-induced granuloma, respectively. The anti-edema effect of MEAC was compared with 10 mg/kg of indomethacin orally. Results: The results suggested that MEAC possess potent anti-inflammatory activity. The acute inflammatory model showed that all the doses of MEAC effectively suppressed the edema produced by histamine, so it may be suggested that its anti-inflammatory activity is possibly backed by its antihistaminic activity. In chronic inflammatory model the effect may be due to the cellular migration to injured sites and accumulation of collagen and mucopolysaccharide. Conclusions: On the basis of these findings, it may be inferred that Anthocephalus cadamba is an anti-inflammatory agent and the results are in agreement with its traditional use.

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Phytochemical Investigation and Anti-Inflammatory Activity of the Leaves of Machilus japonica var. kusanoi

Molecules ◽

10.3390/molecules25184149 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4149

Author(s):

Shiou-Ling Li ◽

Ho-Cheng Wu ◽

Tsong-Long Hwang ◽

Chu-Hung Lin ◽

Shuen-Shin Yang ◽

...

Keyword(s):

Superoxide Anion ◽

2D Nmr ◽

Human Neutrophils ◽

Superoxide Anion Generation ◽

Phytochemical Investigation ◽

Potent Inhibition ◽

Bioassay Guided Fractionation ◽

Ic50 Values ◽

Anti Inflammatory ◽

New Compounds

In a series of anti-inflammatory screenings of lauraceous plants, the methanolic extract of the leaves of Machilus japonica var. kusanoi (Hayata) J.C. Liao showed potent inhibition on both superoxide anion generation and elastase release in human neutrophils. Bioassay-guided fractionation of the leaves of M. japonica var. kusanoi led to the isolation of twenty compounds, including six new butanolides, machinolides A–F (1–6), and fourteen known compounds (7–20). Their structures were characterized by 1D and 2D NMR, UV, IR, CD, and MS data. The absolute configuration of the new compounds were unambiguously confirmed by single-crystal X-ray diffraction analyses (1, 2, and 3) and Mosher’s method (4, 5, and 6). In addition, lignans, (+)-eudesmin (11), (+)-methylpiperitol (12), (+)-pinoresinol (13), and (+)-galbelgin (16) exhibited inhibitory effects on N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-induced superoxide anion generation in human neutrophils with IC50 values of 8.71 ± 0.74 μM, 2.23 ± 0.92 μM, 6.81 ± 1.07 μM, and 7.15 ± 2.26 μM, respectively. The results revealed the anti-inflammatory potentials of Formosan Machilus japonica var. kusanoi.

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